Aspergillus oryzae RIB40



Top Publications

  1. Maruyama J, Escaño C, Kitamoto K. AoSO protein accumulates at the septal pore in response to various stresses in the filamentous fungus Aspergillus oryzae. Biochem Biophys Res Commun. 2010;391:868-73 pubmed publisher
    ..This study presents a novel behavior in which a filamentous fungal protein relocalizes to the septal pore in response to various stresses. ..
  2. Escaño C, Juvvadi P, Jin F, Takahashi T, Koyama Y, Yamashita S, et al. Disruption of the Aopex11-1 gene involved in peroxisome proliferation leads to impaired Woronin body formation in Aspergillus oryzae. Eukaryot Cell. 2009;8:296-305 pubmed publisher
    ..In conclusion, the present study describes the role of Pex11 in Woronin body differentiation for the first time...
  3. Mizuno T, Shiono Y, Koseki T. Biochemical characterization of Aspergillus oryzae native tannase and the recombinant enzyme expressed in Pichia pastoris. J Biosci Bioeng. 2014;118:392-5 pubmed publisher
  4. Vera C, Guerrero C, Illanes A, Conejeros R. A pseudo steady-state model for galacto-oligosaccharides synthesis with ?-galactosidase from Aspergillus oryzae. Biotechnol Bioeng. 2011;108:2270-9 pubmed publisher
    ..It also suggests that the affinity for sugars of the galactosyl-enzyme complex is higher than that of the free enzyme. ..
  5. Wang N, Seko A, Takeda Y, Kikuma T, Ito Y. Cooperative role of calnexin and TigA in Aspergillus oryzae glycoprotein folding. Glycobiology. 2015;25:1090-9 pubmed publisher
    ..The results suggested that in glycoprotein folding process of A. oryzae, TigA plays a similar role as ERp57 in mammalian cells, as a partner protein of AoCNX. ..
  6. Yuasa H, Ball H. Molecular evolution and characterization of fungal indoleamine 2,3-dioxygenases. J Mol Evol. 2011;72:160-8 pubmed publisher
    ..oryzae. The levo-1-methyl tryptophan (L-1MT) is a good inhibitor of both IDO1 and IDO2. However, the activity of fungal IDOs tested was not inhibited at all by L-1MT. ..
  7. Eugster P, Salamin K, Grouzmann E, Monod M. Production and characterization of two major Aspergillus oryzae secreted prolyl endopeptidases able to efficiently digest proline-rich peptides of gliadin. Microbiology. 2015;161:2277-88 pubmed publisher
    ..Digestion of the gliadin peptide in short peptides with both enzymes was found to occur from its N terminus. ..
  8. Nakamura H, Kikuma T, Jin F, Maruyama J, Kitamoto K. AoRim15 is involved in conidial stress tolerance, conidiation and sclerotia formation in the filamentous fungus Aspergillus oryzae. J Biosci Bioeng. 2016;121:365-71 pubmed publisher
    ..Collectively, we demonstrated that AoRim15 is involved in the stress tolerance of conidia and differentially regulates between the two developmental fates of conidiation and sclerotia formation. ..
  9. Tanaka M, Yoshimura M, Ogawa M, Koyama Y, Shintani T, Gomi K. The C2H2-type transcription factor, FlbC, is involved in the transcriptional regulation of Aspergillus oryzae glucoamylase and protease genes specifically expressed in solid-state culture. Appl Microbiol Biotechnol. 2016;100:5859-68 pubmed publisher
    ..These results suggested that FlbC is involved in the transcriptional regulation of genes specifically expressed under solid-state cultivation conditions, possibly independent of the conidiation regulatory network. ..

More Information


  1. Abdel Naby M, El Tanash A, Sherief A. Structural characterization, catalytic, kinetic and thermodynamic properties of Aspergillus oryzae tannase. Int J Biol Macromol. 2016;92:803-811 pubmed publisher
    ..Hydrolysis of tannic acid by the pure enzyme indicated that gallic acid was the end-product. ..
  2. Miyazawa K, Yoshimi A, Zhang S, Sano M, Nakayama M, Gomi K, et al. Increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae by disruption of the genes encoding cell wall ?-1,3-glucan synthase. Biosci Biotechnol Biochem. 2016;80:1853-63 pubmed publisher
  3. Kawaguchi K, Kikuma T, Higuchi Y, Takegawa K, Kitamoto K. Subcellular localization of acyl-CoA binding protein in Aspergillus oryzae is regulated by autophagy machinery. Biochem Biophys Res Commun. 2016;480:8-12 pubmed publisher
    ..Collectively, these results suggest the existence of a regulatory mechanism between AoACBP localization and autophagy. ..
  4. Zhou B, Xie J, Liu X, Wang B, Pan L. Functional and transcriptomic analysis of the key unfolded protein response transcription factor HacA in Aspergillus oryzae. Gene. 2016;593:143-153 pubmed publisher
    ..Collectively, our results suggest that the function of HacA is important not only for UPR induction, but also for growth and fungal physiology, as it serves to reduce secretion stress in A. oryzae. ..
  5. Tadokoro T, Kikuma T, Kitamoto K. Functional analysis of AoAtg11 in selective autophagy in the filamentous fungus Aspergillus oryzae. Fungal Biol. 2015;119:560-7 pubmed publisher
    ..Taken together, these results suggest that AoAtg11 is a selective autophagy-specific protein in A. oryzae, and has distinct molecular functions from that of S. cerevisiae Atg11. ..
  6. Yokota J, Shiro D, Tanaka M, Onozaki Y, Mizutani O, Kakizono D, et al. Cellular responses to the expression of unstable secretory proteins in the filamentous fungus Aspergillus oryzae. Appl Microbiol Biotechnol. 2017;101:2437-2446 pubmed publisher
    ..oryzae) alleviated the UPR induction by mutant MsdS overexpression. These findings suggest that misfolded MsdS and unfolded species of ?-amylase might act synergistically for UPR induction. ..
  7. Suzuki K, Tanaka M, Konno Y, Ichikawa T, Ichinose S, Hasegawa Shiro S, et al. Distinct mechanism of activation of two transcription factors, AmyR and MalR, involved in amylolytic enzyme production in Aspergillus oryzae. Appl Microbiol Biotechnol. 2015;99:1805-15 pubmed publisher
    ..These results suggested that activation of AmyR and MalR is regulated in a different manner, and the preceding activation of MalR is essential for the utilization of maltose as an inducer for AmyR activation. ..
  8. Hunter A, Morris T, Jin B, Saint C, Kelly J. Deletion of creB in Aspergillus oryzae increases secreted hydrolytic enzyme activity. Appl Environ Microbiol. 2013;79:5480-7 pubmed publisher
    ..Collectively, the results for Aspergillus oryzae, Aspergillus nidulans, Trichoderma reesei, and Penicillium decumbens show that deletion of creB may be broadly useful in diverse fungi for increasing production of a variety of enzymes. ..
  9. Hakulinen N, Gasparetti C, Kaljunen H, Kruus K, Rouvinen J. The crystal structure of an extracellular catechol oxidase from the ascomycete fungus Aspergillus oryzae. J Biol Inorg Chem. 2013;18:917-29 pubmed publisher
    ..2-4.3 Å. The UV/vis absorption spectrum of AoCO4 exhibits a distinct maximum of absorbance at 350 nm, which has been reported to be typical of the oxy form of type 3 copper enzymes. ..
  10. Huang H, Maruyama J, Kitamoto K. Aspergillus oryzae AoSO is a novel component of stress granules upon heat stress in filamentous fungi. PLoS ONE. 2013;8:e72209 pubmed publisher
    ..Our results suggest that AoSO is a novel component of stress granules specific to filamentous fungi. The authors would specially like to thank Hiroyuki Nakano and Kei Saeki for generously providing experimental and insightful opinions...
  11. Kuratsu M, Taura A, Shoji J, Kikuchi S, Arioka M, Kitamoto K. Systematic analysis of SNARE localization in the filamentous fungus Aspergillus oryzae. Fungal Genet Biol. 2007;44:1310-23 pubmed publisher
    ..Overall, this analysis allowed us to provide an overview of vesicular trafficking and organelle distribution in A. oryzae...
  12. Jin F, Takahashi T, Matsushima K, Hara S, Shinohara Y, Maruyama J, et al. SclR, a basic helix-loop-helix transcription factor, regulates hyphal morphology and promotes sclerotial formation in Aspergillus oryzae. Eukaryot Cell. 2011;10:945-55 pubmed publisher
  13. Kikuma T, Kitamoto K. Analysis of autophagy in Aspergillus oryzae by disruption of Aoatg13, Aoatg4, and Aoatg15 genes. FEMS Microbiol Lett. 2011;316:61-9 pubmed publisher
    ..In the ?Aoatg15 mutant, autophagic bodies accumulated in vacuoles, suggesting that the uptake process proceeded. We therefore propose that the level of autophagy is closely correlated with the degree of differentiation in A. oryzae...
  14. Zhou S, Fushinobu S, Kim S, Nakanishi Y, Maruyama J, Kitamoto K, et al. Functional analysis and subcellular location of two flavohemoglobins from Aspergillus oryzae. Fungal Genet Biol. 2011;48:200-7 pubmed publisher
    ..These results suggest that FHb1 and FHb2 may play a role in protecting cells from external and internal NO stress, respectively...
  15. Nakahama T, Nakanishi Y, Viscomi A, Takaya K, Kitamoto K, Ottonello S, et al. Distinct enzymatic and cellular characteristics of two secretory phospholipases A2 in the filamentous fungus Aspergillus oryzae. Fungal Genet Biol. 2010;47:318-31 pubmed publisher
    ..These data indicate that two A. oryzae sPLA(2) enzymes display distinct, presumably non-redundant, physiological functions...
  16. Yanagisawa S, Kikuma T, Kitamoto K. Functional analysis of Aoatg1 and detection of the Cvt pathway in Aspergillus oryzae. FEMS Microbiol Lett. 2013;338:168-76 pubmed publisher
    ..Taken together, our findings provide evidence for the existence of the Cvt pathway in A. oryzae...
  17. Koseki T, Mihara K, Murayama T, Shiono Y. A novel Aspergillus oryzae esterase that hydrolyzes 4-hydroxybenzoic acid esters. FEBS Lett. 2010;584:4032-6 pubmed publisher
    ..Finally, the enzyme decreased the antimicrobial activity of parabens against A. oryzae via hydrolysis of the ester bond present in butyl 4-hydroxybenzoic acid...
  18. Yoon J, Aishan T, Maruyama J, Kitamoto K. Enhanced production and secretion of heterologous proteins by the filamentous fungus Aspergillus oryzae via disruption of vacuolar protein sorting receptor gene Aovps10. Appl Environ Microbiol. 2010;76:5718-27 pubmed publisher
    ..These results suggest that AoVps10 plays a role in the regulation of heterologous protein secretion in A. oryzae and may be involved in the vacuolar protein degradation through the Golgi apparatus...
  19. Jin F, Takahashi T, Machida M, Koyama Y. Identification of a basic helix-loop-helix-type transcription regulator gene in Aspergillus oryzae by systematically deleting large chromosomal segments. Appl Environ Microbiol. 2009;75:5943-51 pubmed publisher
    ..The AO090011000215 gene was predicted to encode a helix-loop-helix binding protein belonging to the bHLH family of transcription factors. These results illustrate the potential of the approach for identifying novel functional genes...
  20. Zhou S, Fushinobu S, Nakanishi Y, Kim S, Wakagi T, Shoun H. Cloning and characterization of two flavohemoglobins from Aspergillus oryzae. Biochem Biophys Res Commun. 2009;381:7-11 pubmed publisher
    ..This study is the first to show that a microorganism contains two isozymes of FHb and that intracellular localization of the isozymes could differ...
  21. Koseki T, Hori A, Seki S, Murayama T, Shiono Y. Characterization of two distinct feruloyl esterases, AoFaeB and AoFaeC, from Aspergillus oryzae. Appl Microbiol Biotechnol. 2009;83:689-96 pubmed publisher
    ..Ferulic acid was efficiently released from wheat arabinoxylan when both esterases were applied with xylanase from Thermomyces lanuginosus. Both recombinant proteins also exhibited hydrolytic activity toward chlorogenic acid...
  22. Liu X, Walsh C. Characterization of cyclo-acetoacetyl-L-tryptophan dimethylallyltransferase in cyclopiazonic acid biosynthesis: substrate promiscuity and site directed mutagenesis studies. Biochemistry. 2009;48:11032-44 pubmed publisher
    ..Subsequent site-directed mutagenesis studies identified five polar/charged residues and five tyrosine residues within these motifs that are critical for CpaD activity...
  23. Tanaka M, Hiramoto T, Tada H, Shintani T, Gomi K. Improved α-Amylase Production by Dephosphorylation Mutation of CreD, an Arrestin-Like Protein Required for Glucose-Induced Endocytosis of Maltose Permease and Carbon Catabolite Derepression in Aspergillus oryzae. Appl Environ Microbiol. 2017;83: pubmed publisher
    ..This study shows the dual function of an arrestin-like protein and provides a novel approach for improving the production of amylolytic enzymes in A. oryzae. ..