Aspergillus fumigatus Af293

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Top Publications

  1. Reyes G, Romans A, Nguyen C, May G. Novel mitogen-activated protein kinase MpkC of Aspergillus fumigatus is required for utilization of polyalcohol sugars. Eukaryot Cell. 2006;5:1934-40 pubmed
    ..These studies demonstrated that SakA- and MpkC-dependent patterns of change in mRNA levels are distinct and have minimal overlap in response to these environmental stresses. ..
  2. Cramer R, Perfect B, Pinchai N, Park S, Perlin D, Asfaw Y, et al. Calcineurin target CrzA regulates conidial germination, hyphal growth, and pathogenesis of Aspergillus fumigatus. Eukaryot Cell. 2008;7:1085-97 pubmed publisher
    ..In addition, the importance of CrzA to the production of disease is critical, and thus CrzA is an attractive fungus-specific antifungal target for the treatment of invasive aspergillosis. ..
  3. Henry C, Latge J, Beauvais A. ?1,3 glucans are dispensable in Aspergillus fumigatus. Eukaryot Cell. 2012;11:26-9 pubmed publisher
    ..The lack of ?1,3 glucans affects neither conidial germination nor mycelial vegetative growth and is compensated by an increase in ?1,3 glucan and/or chitin content. ..
  4. Dhingra S, Andes D, Calvo A. VeA regulates conidiation, gliotoxin production, and protease activity in the opportunistic human pathogen Aspergillus fumigatus. Eukaryot Cell. 2012;11:1531-43 pubmed publisher
    ..Although veA affects several cellular processes in A. fumigatus, pathogenicity studies in a neutropenic mouse infection model indicated that veA is dispensable for virulence. ..
  5. Maubon D, Park S, Tanguy M, Huerre M, Schmitt C, Prevost M, et al. AGS3, an alpha(1-3)glucan synthase gene family member of Aspergillus fumigatus, modulates mycelium growth in the lung of experimentally infected mice. Fungal Genet Biol. 2006;43:366-75 pubmed
    ..These results suggest an indirect role for AGS3 in virulence through an adaptive mechanism. ..
  6. Cramer R, Gamcsik M, Brooking R, Najvar L, Kirkpatrick W, Patterson T, et al. Disruption of a nonribosomal peptide synthetase in Aspergillus fumigatus eliminates gliotoxin production. Eukaryot Cell. 2006;5:972-80 pubmed
    ..This result suggests that gliotoxin is not required for virulence in an immunosuppressed host with an invasive pulmonary infection. ..
  7. Zhao W, Panepinto J, Fortwendel J, Fox L, Oliver B, Askew D, et al. Deletion of the regulatory subunit of protein kinase A in Aspergillus fumigatus alters morphology, sensitivity to oxidative damage, and virulence. Infect Immun. 2006;74:4865-74 pubmed
    ..Unregulated PKA signaling is detrimental to the virulence of A. fumigatus, perhaps through the reduced susceptibility of the mutant to damage by oxidizing agents and reduced growth kinetics. ..
  8. Bok J, Chung D, Balajee S, Marr K, Andes D, Nielsen K, et al. GliZ, a transcriptional regulator of gliotoxin biosynthesis, contributes to Aspergillus fumigatus virulence. Infect Immun. 2006;74:6761-8 pubmed
    ..This may indicate that several secondary metabolites are involved in A. fumigatus virulence. ..
  9. Mellado E, Garcia Effron G, Alcazar Fuoli L, Melchers W, Verweij P, Cuenca Estrella M, et al. A new Aspergillus fumigatus resistance mechanism conferring in vitro cross-resistance to azole antifungals involves a combination of cyp51A alterations. Antimicrob Agents Chemother. 2007;51:1897-904 pubmed

More Information

Publications111 found, 100 shown here

  1. Lessing F, Kniemeyer O, Wozniok I, Loeffler J, Kurzai O, Haertl A, et al. The Aspergillus fumigatus transcriptional regulator AfYap1 represents the major regulator for defense against reactive oxygen intermediates but is dispensable for pathogenicity in an intranasal mouse infection model. Eukaryot Cell. 2007;6:2290-302 pubmed
    ..fumigatus wild-type and DeltaAfyap1 mutant germlings by human neutrophilic granulocytes worked equally well irrespective of whether the ROI scavenger glutathione or an NADPH-oxidase inhibitor was added to the cells. ..
  2. Juvvadi P, Fortwendel J, Pinchai N, Perfect B, Heitman J, Steinbach W. Calcineurin localizes to the hyphal septum in Aspergillus fumigatus: implications for septum formation and conidiophore development. Eukaryot Cell. 2008;7:1606-10 pubmed publisher
    ..This is the first study to implicate calcineurin in septum formation and conidiophore development of a filamentous fungus. ..
  3. Qiao J, Liu W, Li R. Truncated Afyap1 attenuates antifungal susceptibility of Aspergillus fumigatus to voriconazole and confers adaptation of the fungus to oxidative stress. Mycopathologia. 2010;170:155-60 pubmed publisher
    ..fumigatus strain. Our study demonstrates that the truncated Afyap1 may involve in antifungal resistance to voriconazole in A. fumigatus and that the truncated Afyap1 sufficiently confers tolerance to oxidative stress in A. fumigatus. ..
  4. Chocklett S, Sobrado P. Aspergillus fumigatus SidA is a highly specific ornithine hydroxylase with bound flavin cofactor. Biochemistry. 2010;49:6777-83 pubmed publisher
    ..NADP(+) is a competitive inhibitor with respect to NADPH, demonstrating that Af SidA forms a ternary complex with NADP(+) and l-ornithine during catalysis. The data suggest that Af SidA likely proceeds by a sequential kinetic mechanism. ..
  5. Steinbach W, Cramer R, Perfect B, Asfaw Y, Sauer T, Najvar L, et al. Calcineurin controls growth, morphology, and pathogenicity in Aspergillus fumigatus. Eukaryot Cell. 2006;5:1091-103 pubmed
    ..Our findings reveal that calcineurin appears to play a globally conserved role in the virulence of several pathogenic fungi and yet plays specialized roles in each and can be an excellent target for therapeutic intervention...
  6. Juvvadi P, Fortwendel J, Rogg L, Burns K, Randell S, Steinbach W. Localization and activity of the calcineurin catalytic and regulatory subunit complex at the septum is essential for hyphal elongation and proper septation in Aspergillus fumigatus. Mol Microbiol. 2011;82:1235-59 pubmed publisher
    ..These findings confirm a cooperative role for the calcineurin complex in regulating hyphal growth and septation...
  7. Franceschini S, Fedkenheuer M, Vogelaar N, Robinson H, Sobrado P, Mattevi A. Structural insight into the mechanism of oxygen activation and substrate selectivity of flavin-dependent N-hydroxylating monooxygenases. Biochemistry. 2012;51:7043-5 pubmed
    ..The structural elucidation of the enzyme in complex with arginine provides insight into the role of electrostatics and hydrogen bonding in the mechanism of oxygen activation in this family of enzymes...
  8. Magnani T, Soriani F, Martins V, Nascimento A, Tudella V, Curti C, et al. Cloning and functional expression of the mitochondrial alternative oxidase of Aspergillus fumigatus and its induction by oxidative stress. FEMS Microbiol Lett. 2007;271:230-8 pubmed publisher
    ..Therefore, positive transformants showed a cyanide-resistant and salicylhydroxamic acid-sensitive respiration, whereas in control cells the oxygen uptake was completely inhibited after KCN addition...
  9. Gastebois A, Blanc Potard A, Gribaldo S, Beau R, Latge J, Mouyna I. Phylogenetic and functional analysis of Aspergillus fumigatus MGTC, a fungal protein homologous to a bacterial virulence factor. Appl Environ Microbiol. 2011;77:4700-3 pubmed publisher
    ..Among eukaryotes, a gene homologous to mgtC was found only in the pathogenic fungus Aspergillus fumigatus. Our data show that the A. fumigatus MgtC (AfuMgtC) protein does not have the same function as the bacterial MgtC proteins. ..
  10. Romero E, Fedkenheuer M, Chocklett S, Qi J, Oppenheimer M, Sobrado P. Dual role of NADP(H) in the reaction of a flavin dependent N-hydroxylating monooxygenase. Biochim Biophys Acta. 2012;1824:850-7 pubmed publisher
    ..Together, these results are consistent with NADPH having a role in flavin reduction and in the modulation of conformational changes, which positions NADP(+) to also play a role in stabilization of the C4a-hydroperoxyflavin. ..
  11. Soukup A, Farnoodian M, Berthier E, Keller N. NosA, a transcription factor important in Aspergillus fumigatus stress and developmental response, rescues the germination defect of a laeA deletion. Fungal Genet Biol. 2012;49:857-65 pubmed publisher
    ..nidulans ?nosA background. Likewise, the A. nidulans nosA allele was able to restore the menadione sensitivity defect of the A. fumigatus ?nosA strain, suggesting conservation of function of the NosA protein in these two species. ..
  12. Zhao W, Lu Y, Ouyang H, Zhou H, Yan J, Du T, et al. N-Glycosylation of Gel1 or Gel2 is vital for cell wall ?-glucan synthesis in Aspergillus fumigatus. Glycobiology. 2013;23:955-68 pubmed publisher
    ..Biochemical analysis revealed that N-glycosylation was essential for the function of Gel1 or Gel2 and thus required for ?-glucan synthesis in A. fumigatus. ..
  13. Wang D, Gricourt M, Arné P, Thierry S, Seguin D, Chermette R, et al. Mutations in the Cyp51A gene and susceptibility to itraconazole in Aspergillus fumigatus isolated from avian farms in France and China. Poult Sci. 2014;93:12-5 pubmed publisher
    ..For 61 randomly selected isolates, the full coding sequence of the Cyp51A gene was determined to detect mutations. Nine amino acid alterations were found in the target enzyme, 3 of which were new. ..
  14. Özmerdiven G, Ak S, Ener B, Ağca H, Cilo B, Tunca B, et al. First determination of azole resistance in Aspergillus fumigatus strains carrying the TR34/L98H mutations in Turkey. J Infect Chemother. 2015;21:581-6 pubmed publisher
    ..8% (n = 66) of isolates. This initial analysis of the resistance mechanism of A. fumigatus from Turkey revealed a common TR34/L98H mutation in the cyp51A gene. ..
  15. Rogg L, Fortwendel J, Juvvadi P, Lilley A, Steinbach W. The chitin synthase genes chsA and chsC are not required for cell wall stress responses in the human pathogen Aspergillus fumigatus. Biochem Biophys Res Commun. 2011;411:549-54 pubmed publisher
    ..Our findings support the existence of complex post-transcriptional regulatory mechanisms controlling chitin biosynthetic machinery in response to cell wall damage. ..
  16. Sakuma M, Imada K, Okumura Y, Uchiya K, Yamashita N, Ogawa K, et al. X-ray structure analysis and characterization of AFUEI, an elastase inhibitor from Aspergillus fumigatus. J Biol Chem. 2013;288:17451-9 pubmed publisher
  17. Shirey C, Badieyan S, Sobrado P. Role of Ser-257 in the sliding mechanism of NADP(H) in the reaction catalyzed by the Aspergillus fumigatus flavin-dependent ornithine N5-monooxygenase SidA. J Biol Chem. 2013;288:32440-8 pubmed publisher
    ..Molecular dynamics simulation results suggest that Ser-257 functions as a pivot point, allowing the nicotinamide of NADP(+) to slide into position for stabilization of the C4a-hydroperoxyflavin. ..
  18. Hargrove T, Wawrzak Z, Lamb D, Guengerich F, Lepesheva G. Structure-Functional Characterization of Cytochrome P450 Sterol 14α-Demethylase (CYP51B) from Aspergillus fumigatus and Molecular Basis for the Development of Antifungal Drugs. J Biol Chem. 2015;290:23916-34 pubmed publisher
  19. Leonardelli F, Macedo D, Dudiuk C, Cabeza M, Gamarra S, Garcia Effron G. Aspergillus fumigatus Intrinsic Fluconazole Resistance Is Due to the Naturally Occurring T301I Substitution in Cyp51Ap. Antimicrob Agents Chemother. 2016;60:5420-6 pubmed publisher
    ..These data strongly implicate the A. fumigatus Cyp51Ap I301 residue in the intrinsic resistance to fluconazole. ..
  20. Wei X, Chen P, Gao R, Li Y, Zhang A, Liu F, et al. Screening and Characterization of a Non-cyp51A Mutation in an Aspergillus fumigatus cox10 Strain Conferring Azole Resistance. Antimicrob Agents Chemother. 2017;61: pubmed publisher
    ..In addition, our study suggests that genome-wide sequencing combined with alignment comparison analysis is an efficient approach to identify the contribution of single nucleotide polymorphism (SNP) diversity to drug resistance. ..
  21. Ma Y, Qiao J, Liu W, Li R. [Effect of sho1 gene of Aspergillus fumigatus on adaptation to osmotic pressure and on sensitivity of antifungal drugs]. Beijing Da Xue Xue Bao Yi Xue Ban. 2009;41:162-7 pubmed
    ..The sho1 gene plays a role in response to osmotic pressure in A. fumigatus, but sho1 has a minor role in the susceptibility to the antifungal drugs despite the slow growth. ..
  22. Winkelströter L, Bom V, de Castro P, Ramalho L, Goldman M, Brown N, et al. High osmolarity glycerol response PtcB phosphatase is important for Aspergillus fumigatus virulence. Mol Microbiol. 2015;96:42-54 pubmed publisher
    ..The ΔptcB strain was avirulent in a murine model of invasive pulmonary aspergillosis. These results stress the importance of the HOG pathway in the regulation of pathogenicity determinants and virulence in A. fumigatus. ..
  23. Moroz O, Maranta M, Shaghasi T, Harris P, Wilson K, Davies G. The three-dimensional structure of the cellobiohydrolase Cel7A from Aspergillus fumigatus at 1.5 Å resolution. Acta Crystallogr F Struct Biol Commun. 2015;71:114-20 pubmed publisher
    ..8 and 1.5 Å, respectively. The product complex with a disaccharide in the +1 and +2 subsites adds to the growing three-dimensional insight into this family of industrially relevant biocatalysts. ..
  24. Shimada T, Suzuki M, Katakura S. Structure of N-myristoyltransferase from Aspergillus fumigatus. Acta Crystallogr D Biol Crystallogr. 2015;71:754-61 pubmed publisher
    ..1 Å. The results advance the understanding of the specificity of NMT inhibitors and provide valuable information for structure-based drug design. ..
  25. Canessa P, Schumacher J, Hevia M, Tudzynski P, Larrondo L. Assessing the effects of light on differentiation and virulence of the plant pathogen Botrytis cinerea: characterization of the White Collar Complex. PLoS ONE. 2013;8:e84223 pubmed publisher
    ..Though overlaps with well-studied fungal systems exist, the light-associated machinery of B. cinerea appears more complex than those of Neurospora crassa and Aspergillus nidulans. ..
  26. Losada L, Sugui J, Eckhaus M, Chang Y, Mounaud S, Figat A, et al. Genetic Analysis Using an Isogenic Mating Pair of Aspergillus fumigatus Identifies Azole Resistance Genes and Lack of MAT Locus's Role in Virulence. PLoS Pathog. 2015;11:e1004834 pubmed publisher
    ..This approach can be applied to multiple areas of interest in A. fumigatus or other heterothallic pathogens, especially for virulence associated traits. ..
  27. El Sayed A, Abdel Azeim S, Ibrahim H, Yassin M, Abdel Ghany S, ESENER S, et al. Biochemical stability and molecular dynamic characterization of Aspergillus fumigatus cystathionine γ-lyase in response to various reaction effectors. Enzyme Microb Technol. 2015;81:31-46 pubmed publisher
    ..Furthermore, electrostatic calculations of selected snapshots of CGL 3D structure under different experimental conditions showed a remarkable differences on the polarity of the enzyme surface. ..
  28. Shwab E, Juvvadi P, Waitt G, Soderblom E, Moseley M, Nicely N, et al. A Novel Phosphoregulatory Switch Controls the Activity and Function of the Major Catalytic Subunit of Protein Kinase A in Aspergillus fumigatus. MBio. 2017;8: pubmed publisher
    ..Our findings represent a divergence in the regulation of PKA signaling in A. fumigatus, which could potentially be exploited as a target and also open the avenue for discovery of fungus-specific downstream effectors of PKA. ..
  29. Fornarotto M, Xiao L, Hou Y, Koch K, Chang E, O Malley R, et al. Sphingolipid biosynthesis in pathogenic fungi: identification and characterization of the 3-ketosphinganine reductase activity of Candida albicans and Aspergillus fumigatus. Biochim Biophys Acta. 2006;1761:52-63 pubmed
    ..Guided by this molecular model, we developed shorter, water-soluble derivatives of 3-ketosphinganine that are substrates for 3-ketosphinganine reductase. ..
  30. Gu W, Yang P, Wang Y, Luo H, Meng K, Wu N, et al. [Mutation research on Q23L and Q23LG272E in phytase derivated from Aspergillus fumigatus]. Sheng Wu Gong Cheng Xue Bao. 2007;23:273-7 pubmed
    ..Analysis of 3-D structure and sequence similarity were used to reveal the presumable factors influencing the enzymatic properties of Q23LG272E, and discussion for the relationship between structure and function of phytase was given. ..
  31. Jiang H, Ouyang H, Zhou H, Jin C. GDP-mannose pyrophosphorylase is essential for cell wall integrity, morphogenesis and viability of Aspergillus fumigatus. Microbiology. 2008;154:2730-9 pubmed publisher
    ..Our results showed that the Afsrb1 gene is essential for cell wall integrity, morphogenesis and viability of Aspergillus fumigatus...
  32. Fuller K, Richie D, Feng X, Krishnan K, Stephens T, Wikenheiser Brokamp K, et al. Divergent Protein Kinase A isoforms co-ordinately regulate conidial germination, carbohydrate metabolism and virulence in Aspergillus fumigatus. Mol Microbiol. 2011;79:1045-62 pubmed publisher
    ..Taken together, these data demonstrate the functional capacity of pkaC2 and emphasize the importance of PKA-mediated metabolic control in the pathogenic potential of A. fumigatus...
  33. Yang F, Ma D, Wan Z, Liu W, Ji Y, Li R. The role of sho1 in polarized growth of Aspergillus fumigatus. Mycopathologia. 2011;172:347-55 pubmed publisher
    ..18-, 0.18- and 0.33-fold of that in the wild type. The sho1 gene affected the polarized growth through affecting the expression of Rho GTPases, the distribution of actin cytoskeleton, vesicle quantity and distribution. ..
  34. Wong Sak Hoi J, Beau R, Latge J. A novel dehydrin-like protein from Aspergillus fumigatus regulates freezing tolerance. Fungal Genet Biol. 2012;49:210-6 pubmed publisher
    ..The DprC-mediated response was dependent on the SakA MAP kinase pathway, and accordingly, SakA phosphorylation was induced by cold shock. When fused to eGFP, the protein was associated with the vacuoles. ..
  35. Magnani Dinamarco T, Brown N, Couto de Almeida R, Alves de Castro P, Savoldi M, de Souza Goldman M, et al. Aspergillus fumigatus calcineurin interacts with a nucleoside diphosphate kinase. Microbes Infect. 2012;14:922-9 pubmed publisher
    ..The SwoH(V83F) mutation did not cause a loss in virulence in the Galleria mellonella infection model. Taken together these results imply that CalA interacts with SwoH. ..
  36. Robinson R, Franceschini S, Fedkenheuer M, Rodríguez P, Ellerbrock J, Romero E, et al. Arg279 is the key regulator of coenzyme selectivity in the flavin-dependent ornithine monooxygenase SidA. Biochim Biophys Acta. 2014;1844:778-84 pubmed publisher
    ..The results indicate that the positive charge at position 279 is critical for tight binding of NADPH and efficient hydroxylation. ..
  37. Herrero S, Takeshita N, Fischer R. F-box protein RcyA controls turnover of the kinesin-7 motor KipA in Aspergillus nidulans. Eukaryot Cell. 2014;13:1085-94 pubmed publisher
    ..These results suggest that RcyA probably labels KipA for degradation and thereby controls the protein amount of KipA. ..
  38. Juvvadi P, Pemble C, Ma Y, Steinbach W. Novel motif in calcineurin catalytic subunit is required for septal localization of calcineurin in Aspergillus fumigatus. FEBS Lett. 2016;590:501-8 pubmed publisher
  39. Jöhnk B, Bayram Ö, Abelmann A, Heinekamp T, Mattern D, Brakhage A, et al. SCF Ubiquitin Ligase F-box Protein Fbx15 Controls Nuclear Co-repressor Localization, Stress Response and Virulence of the Human Pathogen Aspergillus fumigatus. PLoS Pathog. 2016;12:e1005899 pubmed publisher
    ..fumigatus. ..
  40. Bruns S, Kniemeyer O, Hasenberg M, Aimanianda V, Nietzsche S, Thywissen A, et al. Production of extracellular traps against Aspergillus fumigatus in vitro and in infected lung tissue is dependent on invading neutrophils and influenced by hydrophobin RodA. PLoS Pathog. 2010;6:e1000873 pubmed publisher
    ..fumigatus, but suggest that it does not play a major role in killing this fungus. Instead, NETs may have a fungistatic effect and may prevent further spreading...
  41. Robinson R, Badieyan S, Sobrado P. C4a-hydroperoxyflavin formation in N-hydroxylating flavin monooxygenases is mediated by the 2'-OH of the nicotinamide ribose of NADP?. Biochemistry. 2013;52:9089-91 pubmed publisher
    ..This represents a novel role for NADP? in the reaction of flavin-dependent enzymes. ..
  42. Frederick R, Ojha S, LAMB A, Dubois J. How pH modulates the reactivity and selectivity of a siderophore-associated flavin monooxygenase. Biochemistry. 2014;53:2007-16 pubmed publisher
    ..A model is proposed in which L-Lys accelerates H2O2 release via an acid-base mechanism and where side-chain position determines whether H2O2 or the hydroxylation product is observed. ..
  43. Shin K, Kim Y, Yu J. Proteomic analyses reveal the key roles of BrlA and AbaA in biogenesis of gliotoxin in Aspergillus fumigatus. Biochem Biophys Res Commun. 2015;463:428-33 pubmed publisher
    ..fumigatus. ..
  44. Wang P, Choera T, Wiemann P, Pisithkul T, Amador Noguez D, Keller N. TrpE feedback mutants reveal roadblocks and conduits toward increasing secondary metabolism in Aspergillus fumigatus. Fungal Genet Biol. 2016;89:102-113 pubmed publisher
    ..Although icsA had no impact on A. fumigatus Fq production, deletion and over-expression of icsA increased and decreased respectively aromatic amino acid levels suggesting that IcsA can draw from the cellular chorismate pool. ..
  45. Bruder Nascimento A, Dos Reis T, de Castro P, Hori J, Bom V, de Assis L, et al. Mitogen activated protein kinases SakA(HOG1) and MpkC collaborate for Aspergillus fumigatus virulence. Mol Microbiol. 2016;100:841-59 pubmed publisher
    ..We propose that both cell wall integrity (CWI) and HOG pathways collaborate, and that MpkC could act by modulating SakA activity upon exposure to several types of stresses and during CW biosynthesis. ..
  46. Sheppard D, Doedt T, Chiang L, Kim H, Chen D, Nierman W, et al. The Aspergillus fumigatus StuA protein governs the up-regulation of a discrete transcriptional program during the acquisition of developmental competence. Mol Biol Cell. 2005;16:5866-79 pubmed publisher
    ..Additionally, hyphae of the DeltastuA mutant displayed reduced expression of the catalase gene CAT1 and were hypersusceptible to hydrogen peroxide...
  47. Rocha E, Gardiner R, Park S, Martinez Rossi N, Perlin D. A Phe389Leu substitution in ergA confers terbinafine resistance in Aspergillus fumigatus. Antimicrob Agents Chemother. 2006;50:2533-6 pubmed publisher
    ..The equivalent mutation was engineered into the ergA gene of Aspergillus fumigatus, resulting in an F389L substitution that also conferred resistance to this pathogenic mold...
  48. Zhou H, Hu H, Zhang L, Li R, Ouyang H, Ming J, et al. O-Mannosyltransferase 1 in Aspergillus fumigatus (AfPmt1p) is crucial for cell wall integrity and conidium morphology, especially at an elevated temperature. Eukaryot Cell. 2007;6:2260-8 pubmed publisher
    ..In a mouse model, Afpmt1 was not required for the virulence of A. fumigatus under the experimental conditions used...
  49. Lee I, Oh J, Shwab E, Dagenais T, Andes D, Keller N. HdaA, a class 2 histone deacetylase of Aspergillus fumigatus, affects germination and secondary metabolite production. Fungal Genet Biol. 2009;46:782-90 pubmed publisher
    ..A mammalian cell toxicity assay indicated increased activity in the over-expression strain. Neither mutant affected virulence of the fungus as measured by macrophage engulfment of conidia or virulence in a neutropenic mouse model...
  50. Juvvadi P, Gehrke C, Fortwendel J, Lamoth F, Soderblom E, Cook E, et al. Phosphorylation of Calcineurin at a novel serine-proline rich region orchestrates hyphal growth and virulence in Aspergillus fumigatus. PLoS Pathog. 2013;9:e1003564 pubmed publisher
    ..These findings suggest the possibility of harnessing this unique SPRR for innovative antifungal drug design to combat invasive aspergillosis...
  51. Reeves E, Reiber K, Neville C, Scheibner O, Kavanagh K, Doyle S. A nonribosomal peptide synthetase (Pes1) confers protection against oxidative stress in Aspergillus fumigatus. FEBS J. 2006;273:3038-53 pubmed publisher
    ..1. It is concluded that pes1 contributes to improved fungal tolerance against oxidative stress, mediated by the conidial phenotype, during the infection process...
  52. Wong Sak Hoi J, Lamarre C, Beau R, Meneau I, Berepiki A, Barre A, et al. A novel family of dehydrin-like proteins is involved in stress response in the human fungal pathogen Aspergillus fumigatus. Mol Biol Cell. 2011;22:1896-906 pubmed publisher
    ..Accordingly, DprA and DprB were important for peroxisome function. Our findings reveal a novel family of stress-protective proteins in A. fumigatus and, potentially, in filamentous ascomycetes...
  53. O Hanlon K, Cairns T, Stack D, Schrettl M, Bignell E, Kavanagh K, et al. Targeted disruption of nonribosomal peptide synthetase pes3 augments the virulence of Aspergillus fumigatus. Infect Immun. 2011;79:3978-92 pubmed publisher
    ..Proteome alterations in A. fumigatus ?pes3 strongly suggest impaired germination capacity. Uniquely, our data strongly indicate a structural role for the Pes3-encoded NRP, a finding that appears to be novel for an NRP synthetase...
  54. Fortwendel J, Juvvadi P, Rogg L, Asfaw Y, Burns K, Randell S, et al. Plasma membrane localization is required for RasA-mediated polarized morphogenesis and virulence of Aspergillus fumigatus. Eukaryot Cell. 2012;11:966-77 pubmed publisher
    ..Our results demonstrate that plasma membrane-associated RasA is critical for polarized morphogenesis, cell wall stability, and virulence in A. fumigatus...
  55. O Keeffe G, J chl C, Kavanagh K, Doyle S. Extensive proteomic remodeling is induced by eukaryotic translation elongation factor 1B? deletion in Aspergillus fumigatus. Protein Sci. 2013;22:1612-22 pubmed publisher
    ..In addition to this, the strategy of combining targeted gene deletion with comparative proteomics for elucidating the role of proteins of unknown function is further revealed...
  56. Rocha M, Fabri J, Franco de Godoy K, Alves de Castro P, Hori J, Ferreira da Cunha A, et al. Aspergillus fumigatus MADS-Box Transcription Factor rlmA Is Required for Regulation of the Cell Wall Integrity and Virulence. G3 (Bethesda). 2016;6:2983-3002 pubmed publisher
    ..Our results suggest that RlmA functions as a transcription factor in the A. fumigatus CWI pathway, acting downstream of PkcA-MpkA signaling and contributing to the virulence of this fungus. ..
  57. Weber I, Assmann D, Thines E, Steinberg G. Polar localizing class V myosin chitin synthases are essential during early plant infection in the plant pathogenic fungus Ustilago maydis. Plant Cell. 2006;18:225-42 pubmed publisher
    ..Our data show that the polar class IV CHSs are essential for morphogenesis ex planta, whereas the class V myosin-CHS is essential during plant infection...
  58. Tsuchiyama M, Sakamoto T, Fujita T, Murata S, Kawasaki H. Esterification of ferulic acid with polyols using a ferulic acid esterase from Aspergillus niger. Biochim Biophys Acta. 2006;1760:1071-9 pubmed publisher
    ..The ability of 1-glyceryl ferulate to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals was higher than that of the anti-oxidant butyl hydroxytoluene...
  59. Sch bel F, Ibrahim Granet O, Av P, Latg J, Brakhage A, Brock M. Aspergillus fumigatus does not require fatty acid metabolism via isocitrate lyase for development of invasive aspergillosis. Infect Immun. 2007;75:1237-44 pubmed publisher
    ..Therefore, an antifungal drug inhibiting fungal isocitrate lyases, postulated to act against Candida infections, is assumed to be ineffective against A. fumigatus...
  60. Osmani A, Oakley B, Osmani S. Identification and analysis of essential Aspergillus nidulans genes using the heterokaryon rescue technique. Nat Protoc. 2006;1:2517-26 pubmed publisher
    ..Diagnostic PCR can be used to confirm deletions at the molecular level. This technique is suitable for large-scale gene-deletion programs and can be completed within 3 weeks...
  61. Balajee S, Tay S, Lasker B, Hurst S, Rooney A. Characterization of a novel gene for strain typing reveals substructuring of Aspergillus fumigatus across North America. Eukaryot Cell. 2007;6:1392-9 pubmed publisher
    ..fumigatus isolate Af293, whose genome has been sequenced, possesses a CSP gene structure that is substantially different from those of the other A. fumigatus strains studied here, highlighting the need for further taxonomic study...
  62. Sugui J, Pardo J, Chang Y, Zarember K, Nardone G, Galvez E, et al. Gliotoxin is a virulence factor of Aspergillus fumigatus: gliP deletion attenuates virulence in mice immunosuppressed with hydrocortisone. Eukaryot Cell. 2007;6:1562-9 pubmed publisher
    ..Our study confirms that gliotoxin is an important virulence determinant of A. fumigatus and that the type of immunosuppression regimen used is important to reveal the pathogenic potential of gliotoxin...
  63. Sasse C, Bignell E, Hasenberg M, Haynes K, Gunzer M, Braus G, et al. Basal expression of the Aspergillus fumigatus transcriptional activator CpcA is sufficient to support pulmonary aspergillosis. Fungal Genet Biol. 2008;45:693-704 pubmed publisher
    ..Accordingly, basal expression of the Cross-Pathway Control transcriptional activator appears sufficient to support aspergillosis in this disease model...
  64. de Gouv a P, Soriani F, Malavazi I, Savoldi M, Goldman M, Loss O, et al. Functional characterization of the Aspergillus fumigatusPHO80 homologue. Fungal Genet Biol. 2008;45:1135-46 pubmed publisher
    ..mRNA analyses suggest that protein kinase A absence supports the expression of PHO genes in A. fumigatus. Furthermore, DeltaphoB(PHO80) and DeltaphoD(PHO84) mutant are fully virulent in a murine low dose model for invasive aspergillosis...
  65. Schrettl M, Kim H, Eisendle M, Kragl C, Nierman W, Heinekamp T, et al. SreA-mediated iron regulation in Aspergillus fumigatus. Mol Microbiol. 2008;70:27-43 pubmed publisher
    ..As all detrimental effects of sreA disruption are restricted to iron-replete conditions these data underscore that A. fumigatus faces iron-depleted conditions during infection...
  66. Twumasi Boateng K, Yu Y, Chen D, Gravelat F, Nierman W, Sheppard D. Transcriptional profiling identifies a role for BrlA in the response to nitrogen depletion and for StuA in the regulation of secondary metabolite clusters in Aspergillus fumigatus. Eukaryot Cell. 2009;8:104-15 pubmed publisher
    ..Therefore, BrlAp-mediated repression of ribosomal protein gene expression is not downstream of the TOR pathway. Furthermore, inhibition of ribosomal protein gene expression is not sufficient to induce conidiation in the absence of BrlAp...
  67. Levdansky E, Kashi O, Sharon H, Shadkchan Y, Osherov N. The Aspergillus fumigatus cspA gene encoding a repeat-rich cell wall protein is important for normal conidial cell wall architecture and interaction with host cells. Eukaryot Cell. 2010;9:1403-15 pubmed publisher
    ..However, these strains did not exhibit altered virulence in mice with infected lungs. Collectively, these results suggest a role for cspA in maintaining the strength and integrity of the cell wall...
  68. Kotz A, Wagener J, Engel J, Routier F, Echtenacher B, Jacobsen I, et al. Approaching the secrets of N-glycosylation in Aspergillus fumigatus: characterization of the AfOch1 protein. PLoS ONE. 2010;5:e15729 pubmed publisher
    ..Infection experiments provided no evidence for attenuation in virulence; in fact, according to our data the ?afoch1 mutant may even be slightly more virulent than the control strains...
  69. Robbins N, Uppuluri P, Nett J, Rajendran R, Ramage G, Lopez Ribot J, et al. Hsp90 governs dispersion and drug resistance of fungal biofilms. PLoS Pathog. 2011;7:e1002257 pubmed publisher
    ..Thus, we establish a novel mechanism regulating biofilm drug resistance and dispersion and that targeting Hsp90 provides a much-needed strategy for improving clinical outcome in the treatment of biofilm infections...
  70. Dinamarco T, Almeida R, de Castro P, Brown N, Dos Reis T, Ramalho L, et al. Molecular characterization of the putative transcription factor SebA involved in virulence in Aspergillus fumigatus. Eukaryot Cell. 2012;11:518-31 pubmed publisher
    ..A. fumigatus SebA plays a complex role, contributing to several stress tolerance pathways and growth under poor nutritional conditions, and seems to be integrated into different stress responses...
  71. Raymond Bouchard I, Carroll C, Nesbitt J, Henry K, Pinto L, Moinzadeh M, et al. Structural requirements for the activity of the MirB ferrisiderophore transporter of Aspergillus fumigatus. Eukaryot Cell. 2012;11:1333-44 pubmed publisher
    ..The location of MirB was not influenced by the presence of the siderophore TAFC but was sensitive to internal iron stores...
  72. Wang D, Toyotome T, Muraosa Y, Watanabe A, Wuren T, Bunsupa S, et al. GliA in Aspergillus fumigatus is required for its tolerance to gliotoxin and affects the amount of extracellular and intracellular gliotoxin. Med Mycol. 2014;52:506-18 pubmed publisher
    ..fumigatus by exporting the toxin. This also allows the fungus to evade the harmful effect of its own gliotoxin production. Moreover, GliA contributes to the virulence of A. fumigatus through gliotoxin secretion...
  73. Chabane S, Sarfati J, Ibrahim Granet O, Du C, Schmidt C, Mouyna I, et al. Glycosylphosphatidylinositol-anchored Ecm33p influences conidial cell wall biosynthesis in Aspergillus fumigatus. Appl Environ Microbiol. 2006;72:3259-67 pubmed publisher
  74. Bergmann S, Sch mann J, Scherlach K, Lange C, Brakhage A, Hertweck C. Genomics-driven discovery of PKS-NRPS hybrid metabolites from Aspergillus nidulans. Nat Chem Biol. 2007;3:213-7 pubmed publisher
    ..Here we report a new strategy for the successful induction of a silent metabolic pathway in the important model organism Aspergillus nidulans, which led to the discovery of novel PKS-NRPS hybrid metabolites...
  75. Kupfahl C, Michalka A, Lass Fl rl C, Fischer G, Haase G, Ruppert T, et al. Gliotoxin production by clinical and environmental Aspergillus fumigatus strains. Int J Med Microbiol. 2008;298:319-27 pubmed publisher
    ..In contrast, lack of gliotoxin production in the other Aspergillus species tested had no significant influence on the cytotoxic effect of culture supernatant on these immune cells...
  76. Sugui J, Pardo J, Chang Y, M llbacher A, Zarember K, Galvez E, et al. Role of laeA in the Regulation of alb1, gliP, Conidial Morphology, and Virulence in Aspergillus fumigatus. Eukaryot Cell. 2007;6:1552-61 pubmed publisher
    ..Our results suggest that while laeA is not involved in the regulation of alb1 function in conidial morphology, it regulates the synthesis of gliotoxin and the virulence of A. fumigatus...
  77. Herv s Aguilar A, Rodr guez J, Tilburn J, Arst H, Pe alva M. Evidence for the direct involvement of the proteasome in the proteolytic processing of the Aspergillus nidulans zinc finger transcription factor PacC. J Biol Chem. 2007;282:34735-47 pubmed publisher
    ..Wild-type PacC(53) becomes multiply phosphorylated upon alkaline pH exposure. Processing-impaired 4K-->R PacC(53) becomes excessively phosphorylated...
  78. Krahulec S, Armao G, Weber H, Klimacek M, Nidetzky B. Characterization of recombinant Aspergillus fumigatus mannitol-1-phosphate 5-dehydrogenase and its application for the stereoselective synthesis of protio and deuterio forms of D-mannitol 1-phosphate. Carbohydr Res. 2008;343:1414-23 pubmed publisher
    ..1 and 25 degrees C revealed a primary kinetic isotope effect of 2.9+/-0.2, suggesting that the hydride transfer was strongly rate-determining for the overall enzymatic reaction under these conditions...
  79. Qiao J, Kontoyiannis D, Calderone R, Li D, Ma Y, Wan Z, et al. Afyap1, encoding a bZip transcriptional factor of Aspergillus fumigatus, contributes to oxidative stress response but is not essential to the virulence of this pathogen in mice immunosuppressed by cyclophosphamide and triamcinolone. Med Mycol. 2008;46:773-82 pubmed publisher
    ..fumigatus strain in a murine model of invasive pulmonary aspergillosis. Taken together, these results demonstrate that Afyap1 is involved in oxidative stress response but is not an essential virulence factor for A. fumigatus...
  80. L Y, Yang H, Hu H, Wang Y, Rao Z, Jin C. Mutation of Trp137 to glutamate completely removes transglycosyl activity associated with the Aspergillus fumigatus AfChiB1. Glycoconj J. 2009;26:525-34 pubmed publisher
    ..And the M243E showed a remarkable reduction of chitinolytic activity and complete loss of transglycosyl activity. These results suggested that the transglycosyl reaction catalyzed by the AfChiB1 is due to lacking of nucleophile...
  81. Pinchai N, Perfect B, Juvvadi P, Fortwendel J, Cramer R, Asfaw Y, et al. Aspergillus fumigatus calcipressin CbpA is involved in hyphal growth and calcium homeostasis. Eukaryot Cell. 2009;8:511-9 pubmed publisher
    ..fumigatus and provides evidence that CbpA may serve as a feedback inhibitor in some aspects of calcineurin functions...
  82. Metzger U, Schall C, Zocher G, Uns ld I, Stec E, Li S, et al. The structure of dimethylallyl tryptophan synthase reveals a common architecture of aromatic prenyltransferases in fungi and bacteria. Proc Natl Acad Sci U S A. 2009;106:14309-14 pubmed publisher
    ..These bacterial enzymes catalyze the prenylation of aromatic substrates in the biosynthesis of secondary metabolites (i.e., a reaction similar to that of DMATS)...
  83. Valiante V, Jain R, Heinekamp T, Brakhage A. The MpkA MAP kinase module regulates cell wall integrity signaling and pyomelanin formation in Aspergillus fumigatus. Fungal Genet Biol. 2009;46:909-18 pubmed publisher
    ..Interestingly, this MAP kinase module affects the formation of pyomelanin derived from tyrosine degradation...
  84. Shin K, Kwon N, Yu J. Gbetagamma-mediated growth and developmental control in Aspergillus fumigatus. Curr Genet. 2009;55:631-41 pubmed publisher
    ..Northern blot analyses revealed that, while AfulaeA mRNA levels unchanged, accumulation of gliZ mRNA was delayed by DeltaAfusfaD or DeltaAfugpgA. A model summarizing the roles of AfusfaD and AfugpgA in A. fumigatus is presented...
  85. Snelders E, Karawajczyk A, Schaftenaar G, Verweij P, Melchers W. Azole resistance profile of amino acid changes in Aspergillus fumigatus CYP51A based on protein homology modeling. Antimicrob Agents Chemother. 2010;54:2425-30 pubmed publisher
    ..fumigatus isolates is not correlated with azole resistance. Based on the Cyp51A protein homology model, the potential correlation of a mutation to azole resistance can be predicted...
  86. Tao L, Yu J. AbaA and WetA govern distinct stages of Aspergillus fumigatus development. Microbiology. 2011;157:313-26 pubmed publisher
    ..A genetic model depicting the regulation of conidiation in A. fumigatus is proposed...
  87. Li H, Barker B, Grahl N, Puttikamonkul S, Bell J, Craven K, et al. The small GTPase RacA mediates intracellular reactive oxygen species production, polarized growth, and virulence in the human fungal pathogen Aspergillus fumigatus. Eukaryot Cell. 2011;10:174-86 pubmed publisher
    ..These results demonstrate that in vitro growth phenotypes do not always correlate with in vivo virulence and raise intriguing questions about the role of RacA in Aspergillus virulence...
  88. Dinamarco T, Freitas F, Almeida R, Brown N, Dos Reis T, Ramalho L, et al. Functional characterization of an Aspergillus fumigatus calcium transporter (PmcA) that is essential for fungal infection. PLoS ONE. 2012;7:e37591 pubmed publisher
    ..The pmcA-C genes have decreased mRNA abundance into the alveoli in the ?calA and ?crzA mutant strains. However, only the A. fumigatus ?pmcA was avirulent in the murine model of invasive pulmonary aspergillosis...
  89. Camps S, Dutilh B, Arendrup M, Rijs A, Snelders E, Huynen M, et al. Discovery of a HapE mutation that causes azole resistance in Aspergillus fumigatus through whole genome sequencing and sexual crossing. PLoS ONE. 2012;7:e50034 pubmed publisher
    ..fumigatus. The discovery may help understand alternate pathways for azole resistance in A. fumigatus with implications for the molecular diagnosis of resistance and drug discovery...
  90. Chowdhary A, Kathuria S, Xu J, Sharma C, Sundar G, Singh P, et al. Clonal expansion and emergence of environmental multiple-triazole-resistant Aspergillus fumigatus strains carrying the TR??/L98H mutations in the cyp51A gene in India. PLoS ONE. 2012;7:e52871 pubmed publisher
    ..fumigatus to azole fungicides in the environment causes cross-resistance to medical triazoles. The study emphasises the need of continued surveillance of resistance in environmental and clinical A. fumigatus strains...
  91. Shin K, Park H, Kim Y, Yu J. Comparative proteomic analyses reveal that FlbA down-regulates gliT expression and SOD activity in Aspergillus fumigatus. J Proteomics. 2013;87:40-52 pubmed publisher
    ..Our results proposed that FlbA-mediated signaling control negatively affects cellular responses associated with detoxification of reactive oxygen species and exogenous gliotoxin in A. fumigatus...
  92. Fern ndez D, Russi S, Vendrell J, Monod M, Pallar s I. A functional and structural study of the major metalloprotease secreted by the pathogenic fungus Aspergillus fumigatus. Acta Crystallogr D Biol Crystallogr. 2013;69:1946-57 pubmed publisher
    ..fumigatus fungalysin, serving as a framework for the as yet poorly known metallopeptidases from pathogenic fungi...