Genomes and Genes
Candida albicans SC5314
- Zucchi P, Davis T, Kumamoto C. A Candida albicans cell wall-linked protein promotes invasive filamentation into semi-solid medium. Mol Microbiol. 2010;76:733-48 pubmed publisher..Some Dfi1p molecules become cross-linked to the carbohydrate polymers of the cell wall. Thus, Dfi1p is capable of linking the cell wall to the plasma membrane and cytoplasm. ..
- Alonso Monge R, Roman E, Arana D, Prieto D, Urrialde V, Nombela C, et al. The Sko1 protein represses the yeast-to-hypha transition and regulates the oxidative stress response in Candida albicans. Fungal Genet Biol. 2010;47:587-601 pubmed publisher..Collectively, these results implicate Sko1 as an important mediator of the oxidative stress response in C. albicans. ..
- Wimalasena T, Enjalbert B, Guillemette T, Plumridge A, Budge S, Yin Z, et al. Impact of the unfolded protein response upon genome-wide expression patterns, and the role of Hac1 in the polarized growth, of Candida albicans. Fungal Genet Biol. 2008;45:1235-47 pubmed publisher..albicans and in the expression of genes encoding cell surface proteins during ER stress, factors that are important in virulence of this fungal pathogen. ..
- Zacchi L, Gomez Raja J, Davis D. Mds3 regulates morphogenesis in Candida albicans through the TOR pathway. Mol Cell Biol. 2010;30:3695-710 pubmed publisher..Finally, we found that Mds3 and Sit4 coimmunoprecipitate. Thus, Mds3 is a new member of the TOR pathway that contributes to morphogenesis in C. albicans as a regulator of this key morphogenetic pathway. ..
- Aoki W, Kitahara N, Miura N, Morisaka H, Yamamoto Y, Kuroda K, et al. Candida albicans possesses Sap7 as a pepstatin A-insensitive secreted aspartic protease. PLoS ONE. 2012;7:e32513 pubmed publisher..This finding will lead to the development of a novel protease inhibitor beyond pepstatin A. Such a novel inhibitor will be an important research tool as well as pharmaceutical agent for patients suffering from candidiasis. ..
- Oh C, Martin C. Candida albicans Spt23p controls the expression of the Ole1p Delta9 fatty acid desaturase and regulates unsaturated fatty acid biosynthesis. J Biol Chem. 2006;281:7030-9 pubmed..In addition we show caSPT23 is essential for the expression of one of the two Delta9 desaturase homologues in Candida and potentially other functions associated with fatty acid metabolism. ..
- Roman E, Cottier F, Ernst J, Pla J. Msb2 signaling mucin controls activation of Cek1 mitogen-activated protein kinase in Candida albicans. Eukaryot Cell. 2009;8:1235-49 pubmed publisher..albicans and suggest the divergence and specialization of this signaling pathway in filamentous fungi. ..
- Luo S, Poltermann S, Kunert A, Rupp S, Zipfel P. Immune evasion of the human pathogenic yeast Candida albicans: Pra1 is a Factor H, FHL-1 and plasminogen binding surface protein. Mol Immunol. 2009;47:541-50 pubmed publisher..As a released protein, Pra1 enhances complement control in direct vicinity of the yeast and thus generates an additional protective layer which controls host complement attack. ..
- Walker L, Munro C, de Bruijn I, Lenardon M, McKinnon A, Gow N. Stimulation of chitin synthesis rescues Candida albicans from echinocandins. PLoS Pathog. 2008;4:e1000040 pubmed publisher..However, echinocandins and chitin synthase inhibitors synergized strongly, highlighting the potential for combination therapies with greatly enhanced cidal activity. ..
- Adám C, Erdei E, Casado C, Kovacs L, González A, Majoros L, et al. Protein phosphatase CaPpz1 is involved in cation homeostasis, cell wall integrity and virulence of Candida albicans. Microbiology. 2012;158:1258-67 pubmed publisher..albicans. In addition, the germ tube growth rate, and virulence in the BALB/c mouse model, were reduced in the null mutant, suggesting a novel function for CaPpz1 in the yeast to hypha transition that may have medical relevance. ..
- Bonhomme J, Chauvel M, Goyard S, Roux P, Rossignol T, d Enfert C. Contribution of the glycolytic flux and hypoxia adaptation to efficient biofilm formation by Candida albicans. Mol Microbiol. 2011;80:995-1013 pubmed publisher..Thus, adaptation to hypoxia is an integral component of biofilm formation in C. albicans. ..
- Zheng X, Wang Y, Wang Y. Hgc1, a novel hypha-specific G1 cyclin-related protein regulates Candida albicans hyphal morphogenesis. EMBO J. 2004;23:1845-56 pubmed..C. albicans seems to have adapted a conserved strategy to control specifically hyphal morphogenesis. ..
- Cantero P, Ernst J. Damage to the glycoshield activates PMT-directed O-mannosylation via the Msb2-Cek1 pathway in Candida albicans. Mol Microbiol. 2011;80:715-25 pubmed publisher..We propose that a Msb2, Cek1 and Ace2 signalling pathway addresses PMT genes as downstream targets and that different modes of regulation have evolved for PMT1 and PMT2/PMT4 genes. ..
- Sellam A, Tebbji F, Nantel A. Role of Ndt80p in sterol metabolism regulation and azole resistance in Candida albicans. Eukaryot Cell. 2009;8:1174-83 pubmed publisher..albicans. ..
- Boysen J, Fanning S, Newberg J, Murphy R, Mitchell A. Detection of protein-protein interactions through vesicle targeting. Genetics. 2009;182:33-9 pubmed publisher..cerevisiae and the fungal pathogen Candida albicans. We use computational analysis of microscopic images to provide a quantitative and automated assessment of confidence. ..
- Luo G, Ibrahim A, Spellberg B, Nobile C, Mitchell A, Fu Y. Candida albicans Hyr1p confers resistance to neutrophil killing and is a potential vaccine target. J Infect Dis. 2010;201:1718-28 pubmed publisher..albicans, mediating resistance to phagocyte killing. Hyr1p is a promising target for vaccine or other immunological or small molecule intervention to improve the outcomes of disseminated candidiasis. ..
- Gómez Raja J, Davis D. The ?-arrestin-like protein Rim8 is hyperphosphorylated and complexes with Rim21 and Rim101 to promote adaptation to neutral-alkaline pH. Eukaryot Cell. 2012;11:683-93 pubmed publisher..Our analyses provide some insights into potential roles. ..
- Zhang A, Petrov K, Hyun E, Liu Z, Gerber S, Myers L. The Tlo proteins are stoichiometric components of Candida albicans mediator anchored via the Med3 subunit. Eukaryot Cell. 2012;11:874-84 pubmed publisher..This study of caMediator structure provides initial clues to the mechanism of action of the Tlo genes and a platform for further mechanistic studies of caMediator's involvement in gene regulatory patterns that underlie pathogenesis. ..
- Kumamoto C. A contact-activated kinase signals Candida albicans invasive growth and biofilm development. Proc Natl Acad Sci U S A. 2005;102:5576-81 pubmed..Because responding to contact appropriately allows coordinated cellular behavior in a metazoan, commensal C. albicans cells behave like a part of the host, using contact-activated signaling to regulate fungal behavior. ..
- Leach M, Budge S, Walker L, Munro C, Cowen L, Brown A. Hsp90 orchestrates transcriptional regulation by Hsf1 and cell wall remodelling by MAPK signalling during thermal adaptation in a pathogenic yeast. PLoS Pathog. 2012;8:e1003069 pubmed publisher..Therefore Hsp90 modulates the short term Hsf1-mediated activation of the classic heat shock response, coordinating this response with long term thermal adaptation via Mkc1- Hog1- and Cek1-mediated cell wall remodelling. ..
- Uppuluri P, Pierce C, Thomas D, Bubeck S, Saville S, Lopez Ribot J. The transcriptional regulator Nrg1p controls Candida albicans biofilm formation and dispersion. Eukaryot Cell. 2010;9:1531-7 pubmed publisher..Our results demonstrate that manipulation of NRG1 gene expression has a profound influence on biofilm formation and biofilm dispersal, thus identifying Nrg1p as a key regulator of the C. albicans biofilm life cycle. ..
- Sellam A, Askew C, Epp E, Lavoie H, Whiteway M, Nantel A. Genome-wide mapping of the coactivator Ada2p yields insight into the functional roles of SAGA/ADA complex in Candida albicans. Mol Biol Cell. 2009;20:2389-400 pubmed publisher..Furthermore, ADA2 deletion causes a decrease in H3K9 acetylation levels of target genes, thus illustrating its importance for histone acetyl transferase activity. ..
- Nobile C, Andes D, Nett J, Smith F, Yue F, Phan Q, et al. Critical role of Bcr1-dependent adhesins in C. albicans biofilm formation in vitro and in vivo. PLoS Pathog. 2006;2:e63 pubmed..Known adhesins Als1 and Hwp1 also contribute to biofilm formation, as does the novel protein Ece1. ..
- Lopes da Rosa J, Boyartchuk V, Zhu L, Kaufman P. Histone acetyltransferase Rtt109 is required for Candida albicans pathogenesis. Proc Natl Acad Sci U S A. 2010;107:1594-9 pubmed publisher..We conclude that Rtt109 is particularly important for fungal pathogenicity, suggesting a unique target for therapeutic antifungal compounds. ..
- Roy B, Burrack L, Lone M, Berman J, Sanyal K. CaMtw1, a member of the evolutionarily conserved Mis12 kinetochore protein family, is required for efficient inner kinetochore assembly in the pathogenic yeast Candida albicans. Mol Microbiol. 2011;80:14-32 pubmed publisher..We propose that Mis12/Mtw1 proteins also have important co-dependent interactions with inner kinetochore proteins and that these interactions may increase the fidelity of kinetochore formation. ..
- Andaluz E, Bellido A, Gomez Raja J, Selmecki A, Bouchonville K, Calderone R, et al. Rad52 function prevents chromosome loss and truncation in Candida albicans. Mol Microbiol. 2011;79:1462-82 pubmed publisher..We took advantage of the heterozygosity of HIS4 in the Candida albicans SC5314 lineage to study the role of Rad52 in the genomic stability of this important fungal pathogen...
- Aoki W, Kitahara N, Miura N, Morisaka H, Yamamoto Y, Kuroda K, et al. Comprehensive characterization of secreted aspartic proteases encoded by a virulence gene family in Candida albicans. J Biochem. 2011;150:431-8 pubmed publisher..Interestingly, Sap4-6, which are coproduced in the hyphal form, were clustered as the same group, indicating that they may target similar host proteins. These results will lead to further understanding of C. albicans pathogenicity. ..
- Jeeves R, Mason R, Woodacre A, Cashmore A. Ferric reductase genes involved in high-affinity iron uptake are differentially regulated in yeast and hyphae of Candida albicans. Yeast. 2011;28:629-44 pubmed publisher..This demonstrates that the regulation of two virulence determinants, the reductive iron uptake system and the morphological form of C. albicans, are linked...
- Chen C, Pande K, French S, Tuch B, Noble S. An iron homeostasis regulatory circuit with reciprocal roles in Candida albicans commensalism and pathogenesis. Cell Host Microbe. 2011;10:118-35 pubmed publisher..albicans can alternate between genetic programs conferring resistance to iron depletion in the bloodstream versus iron toxicity in the gut, and this may represent a fundamental attribute of gastrointestinal commensal-pathogens. ..
- Ganguly S, Bishop A, Xu W, Ghosh S, Nickerson K, Lanni F, et al. Zap1 control of cell-cell signaling in Candida albicans biofilms. Eukaryot Cell. 2011;10:1448-54 pubmed publisher..In addition, our results indicate that Zap1 is a positive regulator of farnesol accumulation...
- Schild L, Heyken A, De Groot P, Hiller E, Mock M, de Koster C, et al. Proteolytic cleavage of covalently linked cell wall proteins by Candida albicans Sap9 and Sap10. Eukaryot Cell. 2011;10:98-109 pubmed publisher..albicans by human macrophages. We propose that Sap9 and Sap10 influence distinct cell wall functions by proteolytic cleavage of covalently linked cell wall proteins. ..
- Cheetham J, MacCallum D, Doris K, da Silva Dantas A, Scorfield S, Odds F, et al. MAPKKK-independent regulation of the Hog1 stress-activated protein kinase in Candida albicans. J Biol Chem. 2011;286:42002-16 pubmed publisher..This indicates that Hog1 mediates C. albicans virulence by conferring stress resistance rather than regulating morphogenesis. ..
- Kumar R, Chadha S, Saraswat D, Bajwa J, Li R, Conti H, et al. Histatin 5 uptake by Candida albicans utilizes polyamine transporters Dur3 and Dur31 proteins. J Biol Chem. 2011;286:43748-58 pubmed publisher..Understanding of polyamine transporter-mediated internalization of Hst 5 provides new insights into the uptake mechanism for C. albicans toxicity, and further suggests design for targeted fungal therapeutic agents...
- Kaneko A, Umeyama T, Hanaoka N, Monk B, Uehara Y, Niimi M. Tandem affinity purification of the Candida albicans septin protein complex. Yeast. 2004;21:1025-33 pubmed..The successful application of TAP to the purification and analysis of the C. albicans septin complex indicates that this technology will have much wider application to proteomic studies of this pathogenic fungus. ..
- Corvey C, Koetter P, Beckhaus T, Hack J, Hofmann S, Hampel M, et al. Carbon Source-dependent assembly of the Snf1p kinase complex in Candida albicans. J Biol Chem. 2005;280:25323-30 pubmed..Yeast two-hybrid analysis confirmed the interaction partners, and these results showed an activator domain for the CaKis2 protein that has not been reported for S. cerevisiae scaffold subunits. ..
- Kunze D, Melzer I, Bennett D, Sanglard D, MacCallum D, Nörskau J, et al. Functional analysis of the phospholipase C gene CaPLC1 and two unusual phospholipase C genes, CaPLC2 and CaPLC3, of Candida albicans. Microbiology. 2005;151:3381-94 pubmed..These data suggest that C. albicans contains two different classes of phospholipases C which are involved in cellular processes but which have no specific functions in pathogenicity. ..
- Court H, Sudbery P. Regulation of Cdc42 GTPase activity in the formation of hyphae in Candida albicans. Mol Biol Cell. 2007;18:265-81 pubmed..These results suggest the development of hyphal-specific characteristics is promoted by Cdc42-GTP in a process that also requires the intrinsic GTPase activity of Cdc42. ..
- Hsu M, Yu E, Singh S, Lue N. Mutual dependence of Candida albicans Est1p and Est3p in telomerase assembly and activation. Eukaryot Cell. 2007;6:1330-8 pubmed..We also developed an affinity isolation and an in vitro reconstitution protocol for the telomerase complex that will facilitate future mechanistic studies. ..
- Cheetham J, Smith D, da Silva Dantas A, Doris K, Patterson M, Bruce C, et al. A single MAPKKK regulates the Hog1 MAPK pathway in the pathogenic fungus Candida albicans. Mol Biol Cell. 2007;18:4603-14 pubmed..Collectively, our data show that Ssk2 is the sole MAPKKK to relay stress signals to Hog1 in C. albicans and that the MAPK signaling network in C. albicans has diverged significantly from the corresponding network in S. cerevisiae. ..
- Cote P, Whiteway M. The role of Candida albicans FAR1 in regulation of pheromone-mediated mating, gene expression and cell cycle arrest. Mol Microbiol. 2008;68:392-404 pubmed publisher..Thus the C. albicans FAR1 gene is a dosage-dependent regulator of the pheromone response, and is critically required for all aspects of mating process of this pathogenic fungus. ..
- Dunkel N, Blass J, Rogers P, Morschhäuser J. Mutations in the multi-drug resistance regulator MRR1, followed by loss of heterozygosity, are the main cause of MDR1 overexpression in fluconazole-resistant Candida albicans strains. Mol Microbiol. 2008;69:827-40 pubmed publisher..albicans. ..
- Stynen B, Van Dijck P, Tournu H. A CUG codon adapted two-hybrid system for the pathogenic fungus Candida albicans. Nucleic Acids Res. 2010;38:e184 pubmed publisher..We conclude that this system can be used to enhance our knowledge of protein-protein interactions in C. albicans. ..
- Makio T, Nishikawa S, Nakayama T, Nagai H, Endo T. Identification and characterization of a Jem1p ortholog of Candida albicans: dissection of Jem1p functions in karyogamy and protein quality control in Saccharomyces cerevisiae. Genes Cells. 2008;13:1015-26 pubmed publisher..Since the interaction of CaJem1p with Nep98p is weaker than that of ScJem1p, the Nep98p-ScJem1p interaction is likely important for promoting karyogamy in S. cerevisiae. ..
- Song Y, Cheon S, Lee K, Lee S, Lee B, Oh D, et al. Role of the RAM network in cell polarity and hyphal morphogenesis in Candida albicans. Mol Biol Cell. 2008;19:5456-77 pubmed publisher..Collectively, these results demonstrate that the RAM network is critically required for hyphal growth as well as normal vegetative growth in C. albicans. ..
- Sun J, Li W, Jang W, Nayyar N, Sutton M, Edgerton M. Uptake of the antifungal cationic peptide Histatin 5 by Candida albicans Ssa2p requires binding to non-conventional sites within the ATPase domain. Mol Microbiol. 2008;70:1246-60 pubmed..Thus, C. albicans Ssa2p binds Hst 5 at a surface-localized epitope in a subunit of the ATPase domain; and this region is required for intracellular translocation and killing functions of Hst 5. ..
- Wang A, Raniga P, Lane S, Lu Y, Liu H. Hyphal chain formation in Candida albicans: Cdc28-Hgc1 phosphorylation of Efg1 represses cell separation genes. Mol Cell Biol. 2009;29:4406-16 pubmed publisher..Efg1 also displays preferential association with Ace2 target gene promoters during hyphal growth. We show that convergent regulation of Ace2 and Efg1 defines the transcriptional program of cell chain formation. ..
- Chen Y, Montedonico A, Kauffman S, Dunlap J, Menn F, Reynolds T. Phosphatidylserine synthase and phosphatidylserine decarboxylase are essential for cell wall integrity and virulence in Candida albicans. Mol Microbiol. 2010;75:1112-32 pubmed publisher..Cho1p is conserved in fungi, but not mammals, so fungal PS synthase is a potential novel antifungal drug target. ..
- García Prieto F, Gómez Raja J, Andaluz E, Calderone R, Larriba G. Role of the homologous recombination genes RAD51 and RAD59 in the resistance of Candida albicans to UV light, radiomimetic and anti-tumor compounds and oxidizing agents. Fungal Genet Biol. 2010;47:433-45 pubmed publisher..cerevisiae, RAD52 plays a more prominent role than RAD51 in the repair of DSBs in C. albicans and suggest the existence of at least two Rad52-dependent HR pathways, one dependent and one independent of Rad51. ..
- Hnisz D, Majer O, Frohner I, Komnenovic V, Kuchler K. The Set3/Hos2 histone deacetylase complex attenuates cAMP/PKA signaling to regulate morphogenesis and virulence of Candida albicans. PLoS Pathog. 2010;6:e1000889 pubmed publisher..Hence, our work supports a paradigm for manipulating morphogenesis in C. albicans through alternative antifungal therapeutic strategies. ..