Genomes and Genes
Candida albicans SC5314
- Lopes da Rosa J, Boyartchuk V, Zhu L, Kaufman P. Histone acetyltransferase Rtt109 is required for Candida albicans pathogenesis. Proc Natl Acad Sci U S A. 2010;107:1594-9 pubmed publisher..We conclude that Rtt109 is particularly important for fungal pathogenicity, suggesting a unique target for therapeutic antifungal compounds. ..
- Lavoie H, Sellam A, Askew C, Nantel A, Whiteway M. A toolbox for epitope-tagging and genome-wide location analysis in Candida albicans. BMC Genomics. 2008;9:578 pubmed publisher..albicans. This toolbox provides a basic setup to perform purification of protein complexes and increase the number of annotated transcriptional regulators and genetic circuits in C. albicans. ..
- Nicholls S, Leach M, Priest C, Brown A. Role of the heat shock transcription factor, Hsf1, in a major fungal pathogen that is obligately associated with warm-blooded animals. Mol Microbiol. 2009;74:844-61 pubmed publisher..albicans. Therefore, the main role of Hsf1 in this pathogen might be the homeostatic modulation of chaperone levels in response to growth temperature, rather than the activation of acute responses to sudden thermal transitions. ..
- Chen Y, Montedonico A, Kauffman S, Dunlap J, Menn F, Reynolds T. Phosphatidylserine synthase and phosphatidylserine decarboxylase are essential for cell wall integrity and virulence in Candida albicans. Mol Microbiol. 2010;75:1112-32 pubmed publisher..Cho1p is conserved in fungi, but not mammals, so fungal PS synthase is a potential novel antifungal drug target. ..
- Alonso Monge R, Roman E, Arana D, Prieto D, Urrialde V, Nombela C, et al. The Sko1 protein represses the yeast-to-hypha transition and regulates the oxidative stress response in Candida albicans. Fungal Genet Biol. 2010;47:587-601 pubmed publisher..Collectively, these results implicate Sko1 as an important mediator of the oxidative stress response in C. albicans. ..
- Chen C, Pande K, French S, Tuch B, Noble S. An iron homeostasis regulatory circuit with reciprocal roles in Candida albicans commensalism and pathogenesis. Cell Host Microbe. 2011;10:118-35 pubmed publisher..albicans can alternate between genetic programs conferring resistance to iron depletion in the bloodstream versus iron toxicity in the gut, and this may represent a fundamental attribute of gastrointestinal commensal-pathogens. ..
- Cheetham J, MacCallum D, Doris K, da Silva Dantas A, Scorfield S, Odds F, et al. MAPKKK-independent regulation of the Hog1 stress-activated protein kinase in Candida albicans. J Biol Chem. 2011;286:42002-16 pubmed publisher..This indicates that Hog1 mediates C. albicans virulence by conferring stress resistance rather than regulating morphogenesis. ..
- Leach M, Budge S, Walker L, Munro C, Cowen L, Brown A. Hsp90 orchestrates transcriptional regulation by Hsf1 and cell wall remodelling by MAPK signalling during thermal adaptation in a pathogenic yeast. PLoS Pathog. 2012;8:e1003069 pubmed publisher..Therefore Hsp90 modulates the short term Hsf1-mediated activation of the classic heat shock response, coordinating this response with long term thermal adaptation via Mkc1- Hog1- and Cek1-mediated cell wall remodelling. ..
- Rauceo J, Blankenship J, Fanning S, Hamaker J, Deneault J, Smith F, et al. Regulation of the Candida albicans cell wall damage response by transcription factor Sko1 and PAS kinase Psk1. Mol Biol Cell. 2008;19:2741-51 pubmed publisher..Our results show that protein kinase Psk1 is required for expression of SKO1 and of Sko1-dependent genes in response to caspofungin. Thus Psk1 and Sko1 lie in a newly described signal transduction pathway. ..
- Kaneko A, Umeyama T, Hanaoka N, Monk B, Uehara Y, Niimi M. Tandem affinity purification of the Candida albicans septin protein complex. Yeast. 2004;21:1025-33 pubmed..The successful application of TAP to the purification and analysis of the C. albicans septin complex indicates that this technology will have much wider application to proteomic studies of this pathogenic fungus. ..
- Luo S, Poltermann S, Kunert A, Rupp S, Zipfel P. Immune evasion of the human pathogenic yeast Candida albicans: Pra1 is a Factor H, FHL-1 and plasminogen binding surface protein. Mol Immunol. 2009;47:541-50 pubmed publisher..As a released protein, Pra1 enhances complement control in direct vicinity of the yeast and thus generates an additional protective layer which controls host complement attack. ..
- Cheetham J, Smith D, da Silva Dantas A, Doris K, Patterson M, Bruce C, et al. A single MAPKKK regulates the Hog1 MAPK pathway in the pathogenic fungus Candida albicans. Mol Biol Cell. 2007;18:4603-14 pubmed..Collectively, our data show that Ssk2 is the sole MAPKKK to relay stress signals to Hog1 in C. albicans and that the MAPK signaling network in C. albicans has diverged significantly from the corresponding network in S. cerevisiae. ..
- Stynen B, Van Dijck P, Tournu H. A CUG codon adapted two-hybrid system for the pathogenic fungus Candida albicans. Nucleic Acids Res. 2010;38:e184 pubmed publisher..We conclude that this system can be used to enhance our knowledge of protein-protein interactions in C. albicans. ..
- Sellam A, Tebbji F, Nantel A. Role of Ndt80p in sterol metabolism regulation and azole resistance in Candida albicans. Eukaryot Cell. 2009;8:1174-83 pubmed publisher..albicans. ..
- Gómez Raja J, Davis D. The ?-arrestin-like protein Rim8 is hyperphosphorylated and complexes with Rim21 and Rim101 to promote adaptation to neutral-alkaline pH. Eukaryot Cell. 2012;11:683-93 pubmed publisher..Our analyses provide some insights into potential roles. ..
- Boysen J, Fanning S, Newberg J, Murphy R, Mitchell A. Detection of protein-protein interactions through vesicle targeting. Genetics. 2009;182:33-9 pubmed publisher..cerevisiae and the fungal pathogen Candida albicans. We use computational analysis of microscopic images to provide a quantitative and automated assessment of confidence. ..
- Sellam A, Askew C, Epp E, Lavoie H, Whiteway M, Nantel A. Genome-wide mapping of the coactivator Ada2p yields insight into the functional roles of SAGA/ADA complex in Candida albicans. Mol Biol Cell. 2009;20:2389-400 pubmed publisher..Furthermore, ADA2 deletion causes a decrease in H3K9 acetylation levels of target genes, thus illustrating its importance for histone acetyl transferase activity. ..
- Kumamoto C. A contact-activated kinase signals Candida albicans invasive growth and biofilm development. Proc Natl Acad Sci U S A. 2005;102:5576-81 pubmed..Because responding to contact appropriately allows coordinated cellular behavior in a metazoan, commensal C. albicans cells behave like a part of the host, using contact-activated signaling to regulate fungal behavior. ..
- Schild L, Heyken A, De Groot P, Hiller E, Mock M, de Koster C, et al. Proteolytic cleavage of covalently linked cell wall proteins by Candida albicans Sap9 and Sap10. Eukaryot Cell. 2011;10:98-109 pubmed publisher..albicans by human macrophages. We propose that Sap9 and Sap10 influence distinct cell wall functions by proteolytic cleavage of covalently linked cell wall proteins. ..
- da Silva Dantas A, Patterson M, Smith D, MacCallum D, Erwig L, Morgan B, et al. Thioredoxin regulates multiple hydrogen peroxide-induced signaling pathways in Candida albicans. Mol Cell Biol. 2010;30:4550-63 pubmed publisher..albicans systemic infection. Collectively, our data indicate that Trx1 has a multifaceted role in H(2)O(2) signaling and promotes C. albicans survival in the host. ..
- Andaluz E, Bellido A, Gomez Raja J, Selmecki A, Bouchonville K, Calderone R, et al. Rad52 function prevents chromosome loss and truncation in Candida albicans. Mol Microbiol. 2011;79:1462-82 pubmed publisher..We took advantage of the heterozygosity of HIS4 in the Candida albicans SC5314 lineage to study the role of Rad52 in the genomic stability of this important fungal pathogen...
- Bonhomme J, Chauvel M, Goyard S, Roux P, Rossignol T, d Enfert C. Contribution of the glycolytic flux and hypoxia adaptation to efficient biofilm formation by Candida albicans. Mol Microbiol. 2011;80:995-1013 pubmed publisher..Thus, adaptation to hypoxia is an integral component of biofilm formation in C. albicans. ..
- Aoki W, Kitahara N, Miura N, Morisaka H, Yamamoto Y, Kuroda K, et al. Comprehensive characterization of secreted aspartic proteases encoded by a virulence gene family in Candida albicans. J Biochem. 2011;150:431-8 pubmed publisher..Interestingly, Sap4-6, which are coproduced in the hyphal form, were clustered as the same group, indicating that they may target similar host proteins. These results will lead to further understanding of C. albicans pathogenicity. ..
- Zucchi P, Davis T, Kumamoto C. A Candida albicans cell wall-linked protein promotes invasive filamentation into semi-solid medium. Mol Microbiol. 2010;76:733-48 pubmed publisher..Some Dfi1p molecules become cross-linked to the carbohydrate polymers of the cell wall. Thus, Dfi1p is capable of linking the cell wall to the plasma membrane and cytoplasm. ..
- Adám C, Erdei E, Casado C, Kovacs L, González A, Majoros L, et al. Protein phosphatase CaPpz1 is involved in cation homeostasis, cell wall integrity and virulence of Candida albicans. Microbiology. 2012;158:1258-67 pubmed publisher..albicans. In addition, the germ tube growth rate, and virulence in the BALB/c mouse model, were reduced in the null mutant, suggesting a novel function for CaPpz1 in the yeast to hypha transition that may have medical relevance. ..
- Reedy J, Filler S, Heitman J. Elucidating the Candida albicans calcineurin signaling cascade controlling stress response and virulence. Fungal Genet Biol. 2010;47:107-16 pubmed publisher..These studies extend our understanding of the C. albicans calcineurin signaling cascade and its host-niche specific role in virulence. ..
- Roman E, Cottier F, Ernst J, Pla J. Msb2 signaling mucin controls activation of Cek1 mitogen-activated protein kinase in Candida albicans. Eukaryot Cell. 2009;8:1235-49 pubmed publisher..albicans and suggest the divergence and specialization of this signaling pathway in filamentous fungi. ..
- Song Y, Cheon S, Lee K, Lee S, Lee B, Oh D, et al. Role of the RAM network in cell polarity and hyphal morphogenesis in Candida albicans. Mol Biol Cell. 2008;19:5456-77 pubmed publisher..Collectively, these results demonstrate that the RAM network is critically required for hyphal growth as well as normal vegetative growth in C. albicans. ..
- Kunze D, Melzer I, Bennett D, Sanglard D, MacCallum D, Nörskau J, et al. Functional analysis of the phospholipase C gene CaPLC1 and two unusual phospholipase C genes, CaPLC2 and CaPLC3, of Candida albicans. Microbiology. 2005;151:3381-94 pubmed..These data suggest that C. albicans contains two different classes of phospholipases C which are involved in cellular processes but which have no specific functions in pathogenicity. ..
- Corvey C, Koetter P, Beckhaus T, Hack J, Hofmann S, Hampel M, et al. Carbon Source-dependent assembly of the Snf1p kinase complex in Candida albicans. J Biol Chem. 2005;280:25323-30 pubmed..Yeast two-hybrid analysis confirmed the interaction partners, and these results showed an activator domain for the CaKis2 protein that has not been reported for S. cerevisiae scaffold subunits. ..
- Roman E, Nombela C, Pla J. The Sho1 adaptor protein links oxidative stress to morphogenesis and cell wall biosynthesis in the fungal pathogen Candida albicans. Mol Cell Biol. 2005;25:10611-27 pubmed..These results reveal a role for the Sho1 protein in linking oxidative stress, cell wall biogenesis, and morphogenesis in this important human fungal pathogen. ..
- Stichternoth C, Fraund A, Setiadi E, Giasson L, Vecchiarelli A, Ernst J. Sch9 kinase integrates hypoxia and CO2 sensing to suppress hyphal morphogenesis in Candida albicans. Eukaryot Cell. 2011;10:502-11 pubmed publisher..albicans, which suppresses hypha formation but also allows efficient nutrient uptake, metabolism, and virulence. ..
- Roy B, Burrack L, Lone M, Berman J, Sanyal K. CaMtw1, a member of the evolutionarily conserved Mis12 kinetochore protein family, is required for efficient inner kinetochore assembly in the pathogenic yeast Candida albicans. Mol Microbiol. 2011;80:14-32 pubmed publisher..We propose that Mis12/Mtw1 proteins also have important co-dependent interactions with inner kinetochore proteins and that these interactions may increase the fidelity of kinetochore formation. ..
- Aoki W, Kitahara N, Miura N, Morisaka H, Yamamoto Y, Kuroda K, et al. Candida albicans possesses Sap7 as a pepstatin A-insensitive secreted aspartic protease. PLoS ONE. 2012;7:e32513 pubmed publisher..This finding will lead to the development of a novel protease inhibitor beyond pepstatin A. Such a novel inhibitor will be an important research tool as well as pharmaceutical agent for patients suffering from candidiasis. ..
- Oh C, Martin C. Candida albicans Spt23p controls the expression of the Ole1p Delta9 fatty acid desaturase and regulates unsaturated fatty acid biosynthesis. J Biol Chem. 2006;281:7030-9 pubmed..In addition we show caSPT23 is essential for the expression of one of the two Delta9 desaturase homologues in Candida and potentially other functions associated with fatty acid metabolism. ..
- Uppuluri P, Pierce C, Thomas D, Bubeck S, Saville S, Lopez Ribot J. The transcriptional regulator Nrg1p controls Candida albicans biofilm formation and dispersion. Eukaryot Cell. 2010;9:1531-7 pubmed publisher..Our results demonstrate that manipulation of NRG1 gene expression has a profound influence on biofilm formation and biofilm dispersal, thus identifying Nrg1p as a key regulator of the C. albicans biofilm life cycle. ..
- Klippel N, Cui S, Groebe L, Bilitewski U. Deletion of the Candida albicans histidine kinase gene CHK1 improves recognition by phagocytes through an increased exposure of cell wall beta-1,3-glucans. Microbiology. 2010;156:3432-44 pubmed publisher..As there are no homologues of fungal histidine kinases in mammals, Chk1p has to be considered as a promising target for new antifungal agents. ..
- Luo G, Ibrahim A, Spellberg B, Nobile C, Mitchell A, Fu Y. Candida albicans Hyr1p confers resistance to neutrophil killing and is a potential vaccine target. J Infect Dis. 2010;201:1718-28 pubmed publisher..albicans, mediating resistance to phagocyte killing. Hyr1p is a promising target for vaccine or other immunological or small molecule intervention to improve the outcomes of disseminated candidiasis. ..
- Nobile C, Andes D, Nett J, Smith F, Yue F, Phan Q, et al. Critical role of Bcr1-dependent adhesins in C. albicans biofilm formation in vitro and in vivo. PLoS Pathog. 2006;2:e63 pubmed..Known adhesins Als1 and Hwp1 also contribute to biofilm formation, as does the novel protein Ece1. ..
- Hsu M, Yu E, Singh S, Lue N. Mutual dependence of Candida albicans Est1p and Est3p in telomerase assembly and activation. Eukaryot Cell. 2007;6:1330-8 pubmed..We also developed an affinity isolation and an in vitro reconstitution protocol for the telomerase complex that will facilitate future mechanistic studies. ..
- Hoppen J, Dietz M, Warsow G, Rohde R, Schüller H. Ribosomal protein genes in the yeast Candida albicans may be activated by a heterodimeric transcription factor related to Ino2 and Ino4 from S. cerevisiae. Mol Genet Genomics. 2007;278:317-30 pubmed..Since we failed to obtain homozygous deletion mutations for CaINO2 and CaINO4, we conclude that CaIno2 and CaIno4 acquired new essential target genes among which may be ribosomal protein genes. ..
- Wimalasena T, Enjalbert B, Guillemette T, Plumridge A, Budge S, Yin Z, et al. Impact of the unfolded protein response upon genome-wide expression patterns, and the role of Hac1 in the polarized growth, of Candida albicans. Fungal Genet Biol. 2008;45:1235-47 pubmed publisher..albicans and in the expression of genes encoding cell surface proteins during ER stress, factors that are important in virulence of this fungal pathogen. ..
- Zacchi L, Gomez Raja J, Davis D. Mds3 regulates morphogenesis in Candida albicans through the TOR pathway. Mol Cell Biol. 2010;30:3695-710 pubmed publisher..Finally, we found that Mds3 and Sit4 coimmunoprecipitate. Thus, Mds3 is a new member of the TOR pathway that contributes to morphogenesis in C. albicans as a regulator of this key morphogenetic pathway. ..
- Zheng X, Wang Y, Wang Y. Hgc1, a novel hypha-specific G1 cyclin-related protein regulates Candida albicans hyphal morphogenesis. EMBO J. 2004;23:1845-56 pubmed..C. albicans seems to have adapted a conserved strategy to control specifically hyphal morphogenesis. ..
- Walker L, Munro C, de Bruijn I, Lenardon M, McKinnon A, Gow N. Stimulation of chitin synthesis rescues Candida albicans from echinocandins. PLoS Pathog. 2008;4:e1000040 pubmed publisher..However, echinocandins and chitin synthase inhibitors synergized strongly, highlighting the potential for combination therapies with greatly enhanced cidal activity. ..
- Dunkel N, Blass J, Rogers P, Morschhäuser J. Mutations in the multi-drug resistance regulator MRR1, followed by loss of heterozygosity, are the main cause of MDR1 overexpression in fluconazole-resistant Candida albicans strains. Mol Microbiol. 2008;69:827-40 pubmed publisher..albicans. ..
- Jeeves R, Mason R, Woodacre A, Cashmore A. Ferric reductase genes involved in high-affinity iron uptake are differentially regulated in yeast and hyphae of Candida albicans. Yeast. 2011;28:629-44 pubmed publisher..This demonstrates that the regulation of two virulence determinants, the reductive iron uptake system and the morphological form of C. albicans, are linked...
- Ganguly S, Bishop A, Xu W, Ghosh S, Nickerson K, Lanni F, et al. Zap1 control of cell-cell signaling in Candida albicans biofilms. Eukaryot Cell. 2011;10:1448-54 pubmed publisher..In addition, our results indicate that Zap1 is a positive regulator of farnesol accumulation...
- Kumar R, Chadha S, Saraswat D, Bajwa J, Li R, Conti H, et al. Histatin 5 uptake by Candida albicans utilizes polyamine transporters Dur3 and Dur31 proteins. J Biol Chem. 2011;286:43748-58 pubmed publisher..Understanding of polyamine transporter-mediated internalization of Hst 5 provides new insights into the uptake mechanism for C. albicans toxicity, and further suggests design for targeted fungal therapeutic agents...