Campylobacter jejuni subsp. jejuni NCTC 11168


Alias: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819, Campylobacter jejuni subsp. jejuni ATCC 700819, Campylobacter jejuni subsp. jejuni ATCC 700819 = NCTC 11168

Top Publications

  1. Bolla J, Loret E, Zalewski M, Pages J. Conformational analysis of the Campylobacter jejuni porin. J Bacteriol. 1995;177:4266-71 pubmed
    ..coli porins. This study clearly reveals that the C. jejuni MOMP is related to the family of trimeric bacterial porins. ..
  2. Wang Y, Huang W, Taylor D. Cloning and nucleotide sequence of the Campylobacter jejuni gyrA gene and characterization of quinolone resistance mutations. Antimicrob Agents Chemother. 1993;37:457-63 pubmed
    ..The mutation at Thr-86, which is homologous to Ser-83 in Escherichia coli, was associated with high-level resistance to ciprofloxacin in C. jejuni. ..
  3. Garvis S, Tipton S, Konkel M. Identification of a functional homolog of the Escherichia coli and Salmonella typhimurium cysM gene encoding O-acetylserine sulfhydrylase B in Campylobacter jejuni. Gene. 1997;185:63-7 pubmed
    ..These data indicate that the cloned C. jejuni gene is a functional homolog of the cysM gene that codes for O-acetylserine sulfhydrylase B in E. coli and S. typhimurium. ..
  4. Gilbert M, Karwaski M, Bernatchez S, Young N, Taboada E, Michniewicz J, et al. The genetic bases for the variation in the lipo-oligosaccharide of the mucosal pathogen, Campylobacter jejuni. Biosynthesis of sialylated ganglioside mimics in the core oligosaccharide. J Biol Chem. 2002;277:327-37 pubmed
    ..The differences in the LOS outer core structures expressed by the 11 C. jejuni strains examined can be explained by one or more of the five mechanisms described in this work. ..
  5. Hall S, Hitchcock A, Butler C, Kelly D. A Multicopper oxidase (Cj1516) and a CopA homologue (Cj1161) are major components of the copper homeostasis system of Campylobacter jejuni. J Bacteriol. 2008;190:8075-85 pubmed publisher
    ..These observations and the apparent lack of alternative copper tolerance systems suggest that Cj1516 (CueO) and Cj1161 (CopA) are major proteins involved in copper homeostasis in C. jejuni. ..
  6. Hitchen P, Brzostek J, Panico M, Butler J, Morris H, Dell A, et al. Modification of the Campylobacter jejuni flagellin glycan by the product of the Cj1295 homopolymeric-tract-containing gene. Microbiology. 2010;156:1953-62 pubmed publisher
    ..Mass spectrometry demonstrated that the Cj1295 gene is required for glycosylation with the di-O-methylglyceroyl-modified version of pseudaminic acid. ..
  7. Baek K, Vegge C, Skorko Glonek J, Brøndsted L. Different contributions of HtrA protease and chaperone activities to Campylobacter jejuni stress tolerance and physiology. Appl Environ Microbiol. 2011;77:57-66 pubmed publisher
  8. Burucoa C, Fremaux C, Pei Z, Tummuru M, Blaser M, Cenatiempo Y, et al. Nucleotide sequence and characterization of peb4A encoding an antigenic protein in Campylobacter jejuni. Res Microbiol. 1995;146:467-76 pubmed
    ..ORF-B begins 2 bp after the last codon of peb4A and encodes a putative protein of 353 residues with 63.4% identity with E. coli fructose 1,6-biphosphate aldolase. The sequence arrangement suggests that these two genes form an operon. ..
  9. Pickett C, Pesci E, Cottle D, Russell G, Erdem A, Zeytin H. Prevalence of cytolethal distending toxin production in Campylobacter jejuni and relatedness of Campylobacter sp. cdtB gene. Infect Immun. 1996;64:2070-8 pubmed
    ..quot;upsaliensis," and C. hyointestinalis strains, although the HeLa cell assay indicated that these strains make CDT. PCR experiments indicated the probable presence of cdtB sequences in all of these Campylobacter species. ..

More Information

Publications111 found, 100 shown here

  1. Schroder W, Moser I. Primary structure analysis and adhesion studies on the major outer membrane protein of Campylobacter jejuni. FEMS Microbiol Lett. 1997;150:141-7 pubmed
  2. Park S, Purdy D, Leach S. Localized reversible frameshift mutation in the flhA gene confers phase variability to flagellin gene expression in Campylobacter coli. J Bacteriol. 2000;182:207-10 pubmed
    ..Mutation-based phase variation in flhA may generate functional diversity in the host and environment. ..
  3. Goon S, Kelly J, Logan S, Ewing C, Guerry P. Pseudaminic acid, the major modification on Campylobacter flagellin, is synthesized via the Cj1293 gene. Mol Microbiol. 2003;50:659-71 pubmed
    ..Collectively, the data indicate that Cj1293 is essential for Pse5Ac7Ac biosynthesis from UDP-GlcNAc, and that glycosylation is required for flagella biogenesis in campylobacters. ..
  4. Raphael B, Pereira S, Flom G, Zhang Q, Ketley J, Konkel M. The Campylobacter jejuni response regulator, CbrR, modulates sodium deoxycholate resistance and chicken colonization. J Bacteriol. 2005;187:3662-70 pubmed
    ..These data support previous findings that bile resistance contributes to colonization of chickens and establish that the response regulator, CbrR, modulates resistance to bile salts in C. jejuni. ..
  5. Chou W, Dick S, Wakarchuk W, Tanner M. Identification and characterization of NeuB3 from Campylobacter jejuni as a pseudaminic acid synthase. J Biol Chem. 2005;280:35922-8 pubmed
    ..5 +/- 0.7 microM. A mechanistic study on pseudaminic acid synthase, using [2-18O]PEP, shows that catalysis proceeds through a C-O bond cleavage mechanism similar to other PEP condensing synthases such as sialic acid synthase. ..
  6. Guerry P, Ewing C, Schirm M, Lorenzo M, Kelly J, Pattarini D, et al. Changes in flagellin glycosylation affect Campylobacter autoagglutination and virulence. Mol Microbiol. 2006;60:299-311 pubmed
    ..However, there was a qualitative difference in binding patterns to INT407 cells using GFP-labelled 81-176 and mutants lacking PseAm. A mutant lacking PseAm was attenuated in the ferret diarrhoeal disease model. ..
  7. Pittman M, Elvers K, Lee L, Jones M, Poole R, Park S, et al. Growth of Campylobacter jejuni on nitrate and nitrite: electron transport to NapA and NrfA via NrfH and distinct roles for NrfA and the globin Cgb in protection against nitrosative stress. Mol Microbiol. 2007;63:575-90 pubmed
    ..Thus, NrfA and Cgb together provide C. jejuni with constitutive and inducible components of a robust defence against nitrosative stress. ..
  8. Muller A, León Kempis M, Dodson E, Wilson K, Wilkinson A, Kelly D. A bacterial virulence factor with a dual role as an adhesin and a solute-binding protein: the crystal structure at 1.5 A resolution of the PEB1a protein from the food-borne human pathogen Campylobacter jejuni. J Mol Biol. 2007;372:160-71 pubmed
    ..Our results provide a structural basis for understanding both the solute transport and adhesin/virulence functions of PEB1a. ..
  9. Thomas M, Shepherd M, Poole R, van Vliet A, Kelly D, Pearson B. Two respiratory enzyme systems in Campylobacter jejuni NCTC 11168 contribute to growth on L-lactate. Environ Microbiol. 2011;13:48-61 pubmed publisher
    ..The avian and mammalian gut environment contains dense populations of obligate anaerobes that excrete lactate; our data indicate that C. jejuni is well equipped to use L- and D-lactate as both electron-donor and carbon source. ..
  10. Hitchcock A, Hall S, Myers J, Mulholland F, Jones M, Kelly D. Roles of the twin-arginine translocase and associated chaperones in the biogenesis of the electron transport chains of the human pathogen Campylobacter jejuni. Microbiology. 2010;156:2994-3010 pubmed publisher
    ..Surprisingly, despite homology to TorD, Cj1514 was shown to be specifically required for the activity of formate dehydrogenase, not trimethylamine N-oxide reductase, and was designated FdhM. ..
  11. Yao R, Burr D, Doig P, Trust T, Niu H, Guerry P. Isolation of motile and non-motile insertional mutants of Campylobacter jejuni: the role of motility in adherence and invasion of eukaryotic cells. Mol Microbiol. 1994;14:883-93 pubmed
    ..Characterization of the mutants at the molecular level and in animal models should further contribute to our understanding of the pathogenicity of these organisms. ..
  12. Guerry P, Doig P, Alm R, Burr D, Kinsella N, Trust T. Identification and characterization of genes required for post-translational modification of Campylobacter coli VC167 flagellin. Mol Microbiol. 1996;19:369-78 pubmed
    ..This suggests that the surface-exposed post-translational modifications may play a significant role in the protective immune response. ..
  13. Konkel M, Kim B, Klena J, Young C, Ziprin R. Characterization of the thermal stress response of Campylobacter jejuni. Infect Immun. 1998;66:3666-72 pubmed
    ..jejuni thermotolerance. Experiments revealed that a C. jejuni DnaJ mutant was unable to colonize newly hatched Leghorn chickens, suggesting that heat shock proteins play a role in vivo. ..
  14. van Vliet A, Wooldridge K, Ketley J. Iron-responsive gene regulation in a campylobacter jejuni fur mutant. J Bacteriol. 1998;180:5291-8 pubmed
    ..Further analysis of the C. jejuni iron and Fur regulons by using two-dimensional gel electrophoresis demonstrated the total number of iron- and Fur-regulated proteins to be lower than for other bacterial pathogens. ..
  15. Thies F, Karch H, Hartung H, Giegerich G. The ClpB protein from Campylobacter jejuni: molecular characterization of the encoding gene and antigenicity of the recombinant protein. Gene. 1999;230:61-7 pubmed
    ..In ELISA studies, IgA levels reactive to recombinant ClpB were significantly higher in sera of patients with prior C. jejuni infections than in sera obtained from healthy control persons. ..
  16. Konkel M, Kim B, Rivera Amill V, Garvis S. Bacterial secreted proteins are required for the internaliztion of Campylobacter jejuni into cultured mammalian cells. Mol Microbiol. 1999;32:691-701 pubmed
    ..The identification of the C. jejuni ciaB gene represents a significant advance in understanding the molecular mechanism of C. jejuni internalization and the pathogenesis of C. jejuni-mediated enteritis. ..
  17. Baillon M, van Vliet A, Ketley J, Constantinidou C, Penn C. An iron-regulated alkyl hydroperoxide reductase (AhpC) confers aerotolerance and oxidative stress resistance to the microaerophilic pathogen Campylobacter jejuni. J Bacteriol. 1999;181:4798-804 pubmed
    ..The C. jejuni AhpC protein is an important determinant of the ability of this microaerophilic pathogen to survive oxidative and aerobic stress. ..
  18. Jin S, Joe A, Lynett J, Hani E, Sherman P, Chan V. JlpA, a novel surface-exposed lipoprotein specific to Campylobacter jejuni, mediates adherence to host epithelial cells. Mol Microbiol. 2001;39:1225-36 pubmed
    ..A ligand-binding immunoblotting assay showed that JlpA binds to HEp-2 cells, which suggests that JlpA is C. jejuni adhesin. ..
  19. Thibault P, Logan S, Kelly J, Brisson J, Ewing C, Trust T, et al. Identification of the carbohydrate moieties and glycosylation motifs in Campylobacter jejuni flagellin. J Biol Chem. 2001;276:34862-70 pubmed
    ..The mechanism of attachment appears unrelated to a consensus peptide sequence but is rather based on surface accessibility of Ser/Thr residues in the folded protein. ..
  20. Logan S, Kelly J, Thibault P, Ewing C, Guerry P. Structural heterogeneity of carbohydrate modifications affects serospecificity of Campylobacter flagellins. Mol Microbiol. 2002;46:587-97 pubmed
  21. Asakura H, Yamasaki M, Yamamoto S, Igimi S. Deletion of peb4 gene impairs cell adhesion and biofilm formation in Campylobacter jejuni. FEMS Microbiol Lett. 2007;275:278-85 pubmed
    ..A Peb4 homolog has prolyl cis/trans-isomerase activity, suggesting that the loss of this activity in the mutant strain may be responsible for the repression of these proteins. ..
  22. Howard S, Jagannathan A, Soo E, Hui J, Aubry A, Ahmed I, et al. Campylobacter jejuni glycosylation island important in cell charge, legionaminic acid biosynthesis, and colonization of chickens. Infect Immun. 2009;77:2544-56 pubmed publisher
    ..The discovery of molecular mechanisms influencing the persistence of C. jejuni in poultry aids the rational design of approaches to control this problematic pathogen in the food chain. ..
  23. Thies F, Karch H, Hartung H, Giegerich G. Cloning and expression of the dnaK gene of Campylobacter jejuni and antigenicity of heat shock protein 70. Infect Immun. 1999;67:1194-200 pubmed
    ..Antibody responses to rCjDnaK-His were significantly elevated, compared to those of healthy individuals, in about one-third of the serum specimens obtained from C. jejuni enteritis patients. ..
  24. Brás A, Chatterjee S, Wren B, Newell D, Ketley J. A novel Campylobacter jejuni two-component regulatory system important for temperature-dependent growth and colonization. J Bacteriol. 1999;181:3298-302 pubmed
    ..A mutation of the response regulator gene racR reduced the organism's ability to colonize the chicken intestinal tract and resulted in temperature-dependent changes in its protein profile and growth characteristics. ..
  25. Zhang Q, Meitzler J, Huang S, Morishita T. Sequence polymorphism, predicted secondary structures, and surface-exposed conformational epitopes of Campylobacter major outer membrane protein. Infect Immun. 2000;68:5679-89 pubmed
    ..These findings are instrumental in the design of MOMP-based diagnostic tools and vaccines. ..
  26. Brøndsted L, Andersen M, Parker M, Jørgensen K, Ingmer H. The HtrA protease of Campylobacter jejuni is required for heat and oxygen tolerance and for optimal interaction with human epithelial cells. Appl Environ Microbiol. 2005;71:3205-12 pubmed
    ..This defect may be a consequence of the observed altered morphology of the htrA mutant. Thus, our results suggest that in C. jejuni, HtrA is important for growth during stressful conditions and has an impact on virulence. ..
  27. Garenaux A, Guillou S, Ermel G, Wren B, Federighi M, Ritz M. Role of the Cj1371 periplasmic protein and the Cj0355c two-component regulator in the Campylobacter jejuni NCTC 11168 response to oxidative stress caused by paraquat. Res Microbiol. 2008;159:718-26 pubmed publisher
    ..jejuni oxidative stress resistance. ..
  28. Min T, Vedadi M, Watson D, Wasney G, Munger C, Cygler M, et al. Specificity of Campylobacter jejuni adhesin PEB3 for phosphates and structural differences among its ligand complexes. Biochemistry. 2009;48:3057-67 pubmed publisher
    ..On the basis of our findings, we suggest that PEB3 is a transport protein that may function in utilization of 3-PG or other phosphate-containing molecules from the host. ..
  29. Weingarten R, Taveirne M, Olson J. The dual-functioning fumarate reductase is the sole succinate:quinone reductase in Campylobacter jejuni and is required for full host colonization. J Bacteriol. 2009;191:5293-300 pubmed publisher
  30. Mortensen N, Schiellerup P, Boisen N, Klein B, Locht H, Abuoun M, et al. The role of Campylobacter jejuni cytolethal distending toxin in gastroenteritis: toxin detection, antibody production, and clinical outcome. APMIS. 2011;119:626-34 pubmed publisher
    ..These results suggest that CDT does not solely determine severity of infection and clinical outcome. ..
  31. Howlett R, Hughes B, Hitchcock A, Kelly D. Hydrogenase activity in the foodborne pathogen Campylobacter jejuni depends upon a novel ABC-type nickel transporter (NikZYXWV) and is SlyD-independent. Microbiology. 2012;158:1645-55 pubmed publisher
    ..Our data reveal a strict dependence of hydrogenase activity in C. jejuni on high-affinity nickel uptake through an ABC transporter that has distinct properties compared with the E. coli Nik system...
  32. Xie L, Zhang S, Yao W. [Construction of cheA insertion mutant of Campylobacter jejuni and the effect of its adhesion on mice jejunum]. Wei Sheng Wu Xue Bao. 2011;51:208-13 pubmed
    ..The cheA mutant and its complementation were successfully constructed. The cheA gene may play an important role in colonization of C. jejuni on jejunal mucosa of mice. ..
  33. Griffiths P, Park R, Connerton I. The gene for Campylobacter trigger factor: evidence for multiple transcription start sites and protein products. Microbiology. 1995;141 ( Pt 6):1359-67 pubmed
    ..jejuni. The gene has two potential initiation codons, giving rise to two possible nested protein products. Complex differential growth-phase-dependent transcripts give rise to these products. ..
  34. Huang J, Yin Y, Pan Z, Zhang G, Zhu A, Liu X, et al. Intranasal immunization with chitosan/pCAGGS-flaA nanoparticles inhibits Campylobacter jejuni in a White Leghorn model. J Biomed Biotechnol. 2010;2010: pubmed publisher
    ..jejuni strain. This study demonstrated that intranasal delivery of chitosan-DNA vaccine successfully induced effective immune response and might be a promising vaccine candidate against C. jejuni infection. ..
  35. Paek S, Kawai F, Choi K, Yeo H. Crystal structure of the Campylobacter jejuni Cj0090 protein reveals a novel variant of the immunoglobulin fold among bacterial lipoproteins. Proteins. 2012;80:2804-9 pubmed publisher
    ..The structure suggests that Cj0090 may be involved in protein-protein interactions, consistent with a possible role for bacterial lipoproteins. ..
  36. Mavri A, Smole Mozina S. Effects of efflux-pump inducers and genetic variation of the multidrug transporter cmeB in biocide resistance of Campylobacter jejuni and Campylobacter coli. J Med Microbiol. 2013;62:400-11 pubmed publisher
    ..These results indicate that the cmeB gene polymorphism identified is not associated with biocide and erythromycin resistance in Campylobacter. ..
  37. Dolg P, Yao R, Burr D, Guerry P, Trust T. An environmentally regulated pilus-like appendage involved in Campylobacter pathogenesis. Mol Microbiol. 1996;20:885-94 pubmed
    ..However, this mutant, while still possessing the ability to colonize ferrets, caused significantly reduced disease symptoms in this animal model. ..
  38. Bernatchez S, Szymanski C, Ishiyama N, Li J, Jarrell H, Lau P, et al. A single bifunctional UDP-GlcNAc/Glc 4-epimerase supports the synthesis of three cell surface glycoconjugates in Campylobacter jejuni. J Biol Chem. 2005;280:4792-802 pubmed publisher
    ..These data indicate that Gne is the enzyme providing Gal and GalNAc residues with the synthesis of all three cell-surface carbohydrates in C. jejuni NCTC 11168...
  39. Tolkatchev D, Shaykhutdinov R, Xu P, Plamondon J, Watson D, Young N, et al. Three-dimensional structure and ligand interactions of the low molecular weight protein tyrosine phosphatase from Campylobacter jejuni. Protein Sci. 2006;15:2381-94 pubmed
    ..The three-dimensional structure along with mapping of small-molecule binding will be discussed in the context of developing high-affinity inhibitors of this novel LMW-PTP. ..
  40. Rathbun K, Hall J, Thompson S. Cj0596 is a periplasmic peptidyl prolyl cis-trans isomerase involved in Campylobacter jejuni motility, invasion, and colonization. BMC Microbiol. 2009;9:160 pubmed publisher
    ..The cj0596 mutant also expressed altered levels of several proteins. Mutation of cj0596 has an effect on phenotypes related to C. jejuni pathogenesis, probably due to its role in the proper folding of critical outer membrane proteins...
  41. Shaw F, Mulholland F, Le Gall G, Porcelli I, Hart D, Pearson B, et al. Selenium-dependent biogenesis of formate dehydrogenase in Campylobacter jejuni is controlled by the fdhTU accessory genes. J Bacteriol. 2012;194:3814-23 pubmed publisher
    ..The fdhTU genes encode accessory proteins required for FDH expression and activity in C. jejuni, possibly by contributing to acquisition or utilization of selenium...
  42. Yamasaki M, Igimi S, Katayama Y, Yamamoto S, Amano F. Identification of an oxidative stress-sensitive protein from Campylobacter jejuni, homologous to rubredoxin oxidoreductase/rubrerythrin. FEMS Microbiol Lett. 2004;235:57-63 pubmed publisher
  43. Tareen A, Dasti J, Zautner A, Gross U, Lugert R. Campylobacter jejuni proteins Cj0952c and Cj0951c affect chemotactic behaviour towards formic acid and are important for invasion of host cells. Microbiology. 2010;156:3123-35 pubmed publisher
    ..jejuni, (iv) alter the chemotactic behaviour of the pathogen towards formic acid, and (v) are not related to the utilization of formic acid by formate dehydrogenase...
  44. Lei H, Shen Z, Surana P, Routh M, Su C, Zhang Q, et al. Crystal structures of CmeR-bile acid complexes from Campylobacter jejuni. Protein Sci. 2011;20:712-23 pubmed publisher
    ..The results revealed that the regulator binds these acids with dissociation constants in the micromolar region. ..
  45. Holt J, Grant A, Coward C, Maskell D, Quinlan J. Identification of Cj1051c as a major determinant for the restriction barrier of Campylobacter jejuni strain NCTC11168. Appl Environ Microbiol. 2012;78:7841-8 pubmed publisher
    ..The identification of this determinant provides a greater understanding of the molecular biology of C. jejuni as well as a tool for plasmid work with strain NCTC11168. ..
  46. Aliouane S, Pagès J, Bolla J. Cloning, Expression, Purification, Regulation, and Subcellular Localization of a Mini-protein from Campylobacter jejuni. Curr Microbiol. 2016;72:511-7 pubmed publisher
    ..The cysteine residues present in the sequence are probably involved in disulfide bridges, which may promote covalent interactions with other proteins of the Campylobacter envelope. ..
  47. Guerry P, Pope P, Burr D, Leifer J, Joseph S, Bourgeois A. Development and characterization of recA mutants of Campylobacter jejuni for inclusion in attenuated vaccines. Infect Immun. 1994;62:426-32 pubmed
  48. Pawelec D, Jagusztyn Krynicka E. Complete nucleotide sequence of the Campylobacter jejuni 72Dz asd gene. Acta Microbiol Pol. 1996;45:299-304 pubmed
    ..The amino acid sequence of the Asd protein exhibits significant homology to asd gene products from other microorganisms. ..
  49. Korolik V, Fry B, Alderton M, van der Zeijst B, Coloe P. Expression of Campylobacter hyoilei lipo-oligosaccharide (LOS) antigens in Escherichia coli. Microbiology. 1997;143 ( Pt 11):3481-9 pubmed
    ..Both genes are involved in LPS synthesis. The region also contained a sequence homologous to the rfaC gene of E. coli and Sal. typhimurium which is involved in core oligosaccharide synthesis. ..
  50. Klena J, Gray S, Konkel M. Cloning, sequencing, and characterization of the lipopolysaccharide biosynthetic enzyme heptosyltransferase I gene (waaC) from Campylobacter jejuni and Campylobacter coli. Gene. 1998;222:177-85 pubmed
    ..Unlike Escherichia coli and S. typhimurium isolates, the waaC gene in C. jejuni and C. coli isolates does not appear to be linked to the waaF (rfaF) gene. ..
  51. Konkel M, Gray S, Kim B, Garvis S, Yoon J. Identification of the enteropathogens Campylobacter jejuni and Campylobacter coli based on the cadF virulence gene and its product. J Clin Microbiol. 1999;37:510-7 pubmed
    ..These findings indicate that the cadF gene and its product are conserved among C. jejuni and C. coli isolates and that a PCR assay based on the cadF gene may be useful for the detection of Campylobacter organisms in food products...
  52. Korolik V. Aspartate chemosensory receptor signalling in Campylobacter jejuni. Virulence. 2010;1:414-7 pubmed publisher
    ..jejuni chemotactic signalling pathway to be postulated. ..
  53. Grinnage Pulley T, Zhang Q. Genetic Basis and Functional Consequences of Differential Expression of the CmeABC Efflux Pump in Campylobacter jejuni Isolates. PLoS ONE. 2015;10:e0131534 pubmed publisher
    ..Thus, differential expression of CmeABC may facilitate Campylobacter adaptation to antibiotic treatments. ..
  54. Wu Z, Periaswamy B, Sahin O, Yaeger M, PLUMMER P, Zhai W, et al. Point mutations in the major outer membrane protein drive hypervirulence of a rapidly expanding clone of Campylobacter jejuni. Proc Natl Acad Sci U S A. 2016;113:10690-5 pubmed publisher
    ..Furthermore, this study provides general, unbiased experimental and computational approaches that are broadly applicable for efficient elucidation of disease-causing mutations in bacterial pathogens. ..
  55. Miller S, Pesci E, Pickett C. Genetic organization of the region upstream from the Campylobacter jejuni flagellar gene flhA. Gene. 1994;146:31-8 pubmed
    ..subtilis protein contained a nt-binding domain and potential transmembrane (TM) regions. All three ORFs were expressed in E. coli minicells, apparently from their own promoters.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  56. Chan A, Doukov T, Scofield M, Tom Yew S, Ramin A, MacKichan J, et al. Structure and function of P19, a high-affinity iron transporter of the human pathogen Campylobacter jejuni. J Mol Biol. 2010;401:590-604 pubmed publisher
    ..Dimerization is shown to be metal dependent in vitro and is detected in vivo by cross-linking...
  57. Buelow D, Christensen J, Neal McKinney J, Konkel M. Campylobacter jejuni survival within human epithelial cells is enhanced by the secreted protein CiaI. Mol Microbiol. 2011;80:1296-312 pubmed publisher
    ..Based on the data, we conclude that CiaI contributes to the ability of C. jejuni to survive within epithelial cells. ..
  58. Grant K, Park S. Molecular characterization of katA from Campylobacter jejuni and generation of a catalase-deficient mutant of Campylobacter coli by interspecific allelic exchange. Microbiology. 1995;141 ( Pt 6):1369-76 pubmed publisher
    ..coli transformant confirmed that catalase-deficient mutants had arisen via interspecific allelic exchange. Compared to the isogenic parental strain the mutant was more sensitive to killing by H2O2...
  59. Guerry P, Perez Casal J, Yao R, McVeigh A, Trust T. A genetic locus involved in iron utilization unique to some Campylobacter strains. J Bacteriol. 1997;179:3997-4002 pubmed
    ..jejuni examined but did not hybridize to several other strains of C. jejuni, suggesting that the thermophilic campylobacters can be separated into two categories based on the presence of these two iron utilization genes...
  60. Gabrielsen M, Rohdich F, Eisenreich W, Gr wert T, Hecht S, Bacher A, et al. Biosynthesis of isoprenoids: a bifunctional IspDF enzyme from Campylobacter jejuni. Eur J Biochem. 2004;271:3028-35 pubmed publisher
    ..These bifunctional proteins are potential drug targets in several human pathogens (e.g. Helicobacter pylori, C. jejuni and Treponema pallidum)...
  61. Moroz O, Harkiolaki M, Galperin M, Vagin A, Gonz lez Pacanowska D, Wilson K. The crystal structure of a complex of Campylobacter jejuni dUTPase with substrate analogue sheds light on the mechanism and suggests the "basic module" for dimeric d(C/U)TPases. J Mol Biol. 2004;342:1583-97 pubmed publisher
    ..These results in concert with a number of conserved residues responsible for interdomain cross-talk provide valuable insight towards rational drug design...
  62. Luo N, Pereira S, Sahin O, Lin J, Huang S, Michel L, et al. Enhanced in vivo fitness of fluoroquinolone-resistant Campylobacter jejuni in the absence of antibiotic selection pressure. Proc Natl Acad Sci U S A. 2005;102:541-6 pubmed publisher
  63. Jeon B, Zhang Q. Cj0011c, a periplasmic single- and double-stranded DNA-binding protein, contributes to natural transformation in Campylobacter jejuni. J Bacteriol. 2007;189:7399-407 pubmed publisher
    ..jejuni. These results indicate that Cj0011c functions as a periplasmic DNA receptor contributing to the natural transformation of C. jejuni...
  64. Holmes C, Penn C, Lund P. The hrcA and hspR regulons of Campylobacter jejuni. Microbiology. 2010;156:158-66 pubmed publisher
    ..Strains mutated in hspR, but not those mutated in hrcA, showed enhanced thermotolerance. Some genes previously identified as being downregulated in a strain lacking hspR showed no change in expression in our experiments...
  65. Zampronio C, Blackwell G, Penn C, Cooper H. Novel glycosylation sites localized in Campylobacter jejuni flagellin FlaA by liquid chromatography electron capture dissociation tandem mass spectrometry. J Proteome Res. 2011;10:1238-45 pubmed publisher
    ..For comparison, on-line liquid chromatography collision-induced dissociation of the tryptic digests was performed, but it was not possible to assign sites of glycosylation by that method...
  66. Pei Z, Blaser M. PEB1, the major cell-binding factor of Campylobacter jejuni, is a homolog of the binding component in gram-negative nutrient transport systems. J Biol Chem. 1993;268:18717-25 pubmed
    ..8%) and hisJ (28.9%), whereas ORF C has nearly 50% identity to glnQ and hisP. Thus, PEB1 could be involved both in binding to intestinal cells and in amino acid transport...
  67. Thies F, Weishaupt A, Karch H, Hartung H, Giegerich G. Cloning, sequencing and molecular analysis of the Campylobacter jejuni groESL bicistronic operon. Microbiology. 1999;145 ( Pt 1):89-98 pubmed publisher
  68. Gilbert M, Brisson J, Karwaski M, Michniewicz J, Cunningham A, Wu Y, et al. Biosynthesis of ganglioside mimics in Campylobacter jejuni OH4384. Identification of the glycosyltransferase genes, enzymatic synthesis of model compounds, and characterization of nanomole amounts by 600-mhz (1)h and (13)c NMR analysis. J Biol Chem. 2000;275:3896-906 pubmed
    ..jejuni OH4384 were confirmed by comparing their sequence and activity with corresponding homologues in two related C. jejuni strains that express shorter ganglioside mimics in their LOS...
  69. Holmes K, Mulholland F, Pearson B, Pin C, McNicholl Kennedy J, Ketley J, et al. Campylobacter jejuni gene expression in response to iron limitation and the role of Fur. Microbiology. 2005;151:243-57 pubmed publisher
    ..These results provide new insights into the effects of iron on metabolism and oxidative stress response as well as the regulatory role of Fur...
  70. Mileni M, MacMillan F, Tziatzios C, Zwicker K, Haas A, M ntele W, et al. Heterologous production in Wolinella succinogenes and characterization of the quinol:fumarate reductase enzymes from Helicobacter pylori and Campylobacter jejuni. Biochem J. 2006;395:191-201 pubmed publisher
    ..pylori and C. jejuni. This characterization allows, for the first time, a detailed comparison of the QFR enzymes from C. jejuni and H. pylori with that of W. succinogenes...
  71. Vijayakumar S, Merkx Jacques A, Ratnayake D, Gryski I, Obhi R, Houle S, et al. Cj1121c, a novel UDP-4-keto-6-deoxy-GlcNAc C-4 aminotransferase essential for protein glycosylation and virulence in Campylobacter jejuni. J Biol Chem. 2006;281:27733-43 pubmed publisher
    ..Finally, we show that cj1121c is necessary for protein glycosylation by lectin Western blotting. Collectively, these results validate Cj1121c as a promising drug target and provide the means to assay for inhibitors...
  72. Cagliero C, Cloix L, Cloeckaert A, Payot S. High genetic variation in the multidrug transporter cmeB gene in Campylobacter jejuni and Campylobacter coli. J Antimicrob Chemother. 2006;58:168-72 pubmed publisher
    ..The sequence data obtained in this study will help to design suitable tools to study the presence or the expression of the gene cmeB...
  73. Pawelec D, Rozynek E, Popowski J, Jagusztyn Krynicka E. Cloning and characterization of a Campylobacter jejuni 72Dz/92 gene encoding a 30 kDa immunopositive protein, component of the ABC transport system; expression of the gene in avirulent Salmonella typhimurium. FEMS Immunol Med Microbiol. 1997;19:137-50 pubmed
    ..The gene was introduced into avirulent Salmonella typhimurium vaccine strain where it is expressed at a reasonably high level...
  74. Karlyshev A, Linton D, Gregson N, Lastovica A, Wren B. Genetic and biochemical evidence of a Campylobacter jejuni capsular polysaccharide that accounts for Penner serotype specificity. Mol Microbiol. 2000;35:529-41 pubmed
    ..We demonstrate for the first time that the previously described O-antigen of C. jejuni is a capsular polysaccharide and a common component of the thermostable antigen used for serotyping of C. jejuni...
  75. Pawelec D, Korsak D, Wyszyńska A, Rozynek E, Popowski J, Jagusztyn Krynicka E. Genetic diversity of the Campylobacter genes coding immunodominant proteins. FEMS Microbiol Lett. 2000;185:43-9 pubmed
    ..Most of the observed nucleotide substitutions occurred at the third base of the codons. This observation is consistent with the results of Western blot experiments...
  76. Chance M, Fiser A, Sali A, Pieper U, Eswar N, Xu G, et al. High-throughput computational and experimental techniques in structural genomics. Genome Res. 2004;14:2145-54 pubmed publisher
    ..This projected resource will provide structural biology information important to understanding the function of most proteins of the cell...
  77. Muller A, Thomas G, Horler R, Brannigan J, Blagova E, Levdikov V, et al. An ATP-binding cassette-type cysteine transporter in Campylobacter jejuni inferred from the structure of an extracytoplasmic solute receptor protein. Mol Microbiol. 2005;57:143-55 pubmed
    ..These data imply that Cj0982 is the binding protein component of an ABC-type cysteine transporter system and that cysteine uptake is important in the physiology of C. jejuni...
  78. Leon Kempis M, Guccione E, Mulholland F, Williamson M, Kelly D. The Campylobacter jejuni PEB1a adhesin is an aspartate/glutamate-binding protein of an ABC transporter essential for microaerobic growth on dicarboxylic amino acids. Mol Microbiol. 2006;60:1262-75 pubmed
    ..It is concluded that in addition to the established role of PEB1a as an adhesin, the PEB1 transport system plays a key role in the utilization of aspartate and glutamate, which may be important in vivo carbon sources for this pathogen...
  79. Fry B, Korolik V, ten Brinke J, Pennings M, Zalm R, Teunis B, et al. The lipopolysaccharide biosynthesis locus of Campylobacter jejuni 81116. Microbiology. 1998;144 ( Pt 8):2049-61 pubmed
    ..They consisted of three homologues of sugar biosynthesis genes, two homologues of transport genes and six homologues of sugar transferases...
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    ..Affinity-purified FLAG-tagged Cj0143c binds zinc in vitro. Based on our findings and on its homology to E. coli ZnuA, we conclude that Cj0143c encodes the C. jejuni orthologue of ZnuA...
  81. Bustamante V, Puente J, Sánchez López F, Bobadilla M, Calva E. Identification of Campylobacter jejuni and C. coli using the rpoB gene and a cryptic DNA fragment from C. jejuni. Gene. 1995;165:1-8 pubmed
    ..The most conserved segments correspond to putative functional domains. ..
  82. Pesci E, Cottle D, Pickett C. Genetic, enzymatic, and pathogenic studies of the iron superoxide dismutase of Campylobacter jejuni. Infect Immun. 1994;62:2687-94 pubmed
    ..Finally, the ability of the 81-176 sodB strain to survive INT407 cell invasion was found to be significantly decreased (12-fold) compared with that of the parent, suggesting a potential role for SodB in C. jejuni intracellular survival...
  83. Konkel M, Marconi R, Mead D, Cieplak W. Cloning and expression of the hup encoding a histone-like protein of Campylobacter jejuni. Gene. 1994;146:83-6 pubmed
    ..coli and Bacillus promoters. Southern hybridization analyses indicate that C. jejuni has a single copy of hup...
  84. Wai S, Nakayama K, Umene K, Moriya T, Amako K. Construction of a ferritin-deficient mutant of Campylobacter jejuni: contribution of ferritin to iron storage and protection against oxidative stress. Mol Microbiol. 1996;20:1127-34 pubmed
    ..These findings demonstrate that ferritin in C. jejuni makes a significant contribution to both iron storage and protection from intracellular iron overload, and resulting iron-mediated oxidative stress...
  85. W sten M, Boeve M, Gaastra W, van der Zeijst B. Cloning and characterization of the gene encoding the primary sigma-factor of Campylobacter jejuni. FEMS Microbiol Lett. 1998;162:97-103 pubmed
    ..A C. jejuni sigma 70 promoter, not recognized by the E. coli sigma 70 factor, could be activated in this bacterium in the presence of the cloned C. jejuni RpoD protein...
  86. Linton D, Karlyshev A, Wren B. Deciphering Campylobacter jejuni cell surface interactions from the genome sequence. Curr Opin Microbiol. 2001;4:35-40 pubmed
    ..jejuni, a C. jejuni protein that is translocated into eukaryotic cells, and plasmid-encoded components of a putative type IV secretion system are likely to be significant in terms of the host-pathogen interaction...
  87. Misawa N, Kawashima K, Kondo F, Allos B, Blaser M. DNA diversity of the wla gene cluster among serotype HS:19 and non-HS:19 Campylobacter jejuni strains. J Endotoxin Res. 2001;7:349-58 pubmed
    ..RFLP analysis of wla genes also may be useful for epidemiological studies. ..
  88. Han J, Sahin O, Barton Y, Zhang Q. Key role of Mfd in the development of fluoroquinolone resistance in Campylobacter jejuni. PLoS Pathog. 2008;4:e1000083 pubmed publisher
  89. Carswell C, Rigden M, Baenziger J. Expression, purification, and structural characterization of CfrA, a putative iron transporter from Campylobacter jejuni. J Bacteriol. 2008;190:5650-62 pubmed publisher
    ..The sequence variations suggest that there are differences in the mechanisms used by CfrA and FepA to interact with bacterial siderophores. It may be possible to exploit these structural differences to develop CfrA-specific therapeutics...
  90. Tourigny D, Elliott P, Edgell L, Hudson G, Moody P. Expression, purification, crystallization and preliminary X-ray analysis of wild-type and of an active-site mutant of glyceraldehyde-3-phosphate dehydrogenase from Campylobacter jejuni. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011;67:72-5 pubmed publisher
    ..9?Å resolution. The space group is shown to be I4(1)22, with unit-cell parameters a=90.75, b=90.75, c=225.48?Å, ?=90.46, ?=90.46, ?=222.79°; each asymmetric unit contains only one subunit of the tetrameric enzyme. ..
  91. Kim M, Hwang S, Ryu S, Jeon B. Regulation of perR expression by iron and PerR in Campylobacter jejuni. J Bacteriol. 2011;193:6171-8 pubmed publisher
    ..The results of this study demonstrated that PerR directly interacts with the perR promoter and regulates perR transcription and that perR autoregulation is responsible for the repression of perR transcription by iron in C. jejuni...
  92. Flint A, Sun Y, Stintzi A. Cj1386 is an ankyrin-containing protein involved in heme trafficking to catalase in Campylobacter jejuni. J Bacteriol. 2012;194:334-45 pubmed publisher
    ..These results indicate an important role for Cj1386 in Campylobacter colonization and pathogenesis...