Clostridium acetobutylicum ATCC 824

Summary

Alias: Clostridium acetobutylicum DSM 792, Clostridium acetobutylicum str. ATCC 824, Clostridium acetobutylicum strain ATCC 824

Top Publications

  1. Thompson J, Hess S, Pikis A. Genes malh and pagl of Clostridium acetobutylicum ATCC 824 encode NAD+- and Mn2+-dependent phospho-alpha-glucosidase(s). J Biol Chem. 2004;279:1553-61 pubmed publisher
    ..Of the two putative operons, our findings suggest that only proteins encoded by the mal operon participate in the dissimilation of maltose and related O-alpha-linked glucosides by C. acetobutylicum 824...
  2. Boynton Z, Bennett G, Rudolph F. Cloning, sequencing, and expression of genes encoding phosphotransacetylase and acetate kinase from Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol. 1996;62:2758-66 pubmed
    ..The results from overexpression of ack and pta in C. acetobutylicum showed that the final ratios of acetate to other major products were higher and that there was a greater proportion of two- versus four-carbon-derived products...
  3. Gorwa M, Croux C, Soucaille P. Molecular characterization and transcriptional analysis of the putative hydrogenase gene of Clostridium acetobutylicum ATCC 824. J Bacteriol. 1996;178:2668-75 pubmed
    A 2.8-kbp DNA region of Clostridium acetobutylicum ATCC 824 containing the putative hydrogenase gene (hydA) was cloned and sequenced...
  4. Kutty R, Bennett G. Biochemical characterization of trinitrotoluene transforming oxygen-insensitive nitroreductases from Clostridium acetobutylicum ATCC 824. Arch Microbiol. 2005;184:158-67 pubmed publisher
    ..Both the nitroreductases were inhibited by dicoumarol and salicyl hydroxamate. The nitroreductases showed higher relative expression on induction with TNT, nitrofurazone and nitrofurantoin compared to the uninduced control...
  5. Belouski E, Watson D, Bennett G. Cloning, sequence, and expression of the phosphofructokinase gene of Clostridium acetobutylicum ATCC 824 in Escherichia coli. Curr Microbiol. 1998;37:17-22 pubmed
    The pfk gene encoding phosphofructokinase (Pfk) from the anaerobic bacterium Clostridium acetobutylicum ATCC 824 was cloned and sequenced. The gene was identified in a plasmid library by complementation of an E...
  6. Jones S, Tracy B, Gaida S, Papoutsakis E. Inactivation of ?F in Clostridium acetobutylicum ATCC 824 blocks sporulation prior to asymmetric division and abolishes ?E and ?G protein expression but does not block solvent formation. J Bacteriol. 2011;193:2429-40 pubmed publisher
    ..acetobutylicum chromosome--with the aim of altering cell metabolism and differentiation...
  7. Nair R, Bennett G, Papoutsakis E. Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824. J Bacteriol. 1994;176:871-85 pubmed
    ..Petersen, E. T. Papoutsakis, and G. N. Bennett, Appl. Environ. Microbiol. 56:1576-1583, 1990) of Clostridium acetobutylicum ATCC 824 and sequenced. The 2,619-bp aad codes for a 96,517-Da protein...
  8. Walter K, Nair R, Cary J, Bennett G, Papoutsakis E. Sequence and arrangement of two genes of the butyrate-synthesis pathway of Clostridium acetobutylicum ATCC 824. Gene. 1993;134:107-11 pubmed
    The genes encoding both Clostridium acetobutylicum ATCC 824 butyrate synthesis pathway enzymes, phosphotransbutyrylase (ptb) and butyrate kinase (buk), were sequenced...
  9. Bi C, Jones S, Hess D, Tracy B, Papoutsakis E. SpoIIE is necessary for asymmetric division, sporulation, and expression of sigmaF, sigmaE, and sigmaG but does not control solvent production in Clostridium acetobutylicum ATCC 824. J Bacteriol. 2011;193:5130-7 pubmed publisher
    ..Thus, we concluded that SpoIIE does not control solvent formation...
  10. Nair R, Green E, Watson D, Bennett G, Papoutsakis E. Regulation of the sol locus genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 by a putative transcriptional repressor. J Bacteriol. 1999;181:319-30 pubmed
    ..a repressor of the sol locus (aad, ctfA, ctfB and adc) genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824. Primer extension analysis identified a transcriptional start site 35 bp upstream of the solR start ..

Detail Information

Publications67

  1. Thompson J, Hess S, Pikis A. Genes malh and pagl of Clostridium acetobutylicum ATCC 824 encode NAD+- and Mn2+-dependent phospho-alpha-glucosidase(s). J Biol Chem. 2004;279:1553-61 pubmed publisher
    ..Of the two putative operons, our findings suggest that only proteins encoded by the mal operon participate in the dissimilation of maltose and related O-alpha-linked glucosides by C. acetobutylicum 824...
  2. Boynton Z, Bennett G, Rudolph F. Cloning, sequencing, and expression of genes encoding phosphotransacetylase and acetate kinase from Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol. 1996;62:2758-66 pubmed
    ..The results from overexpression of ack and pta in C. acetobutylicum showed that the final ratios of acetate to other major products were higher and that there was a greater proportion of two- versus four-carbon-derived products...
  3. Gorwa M, Croux C, Soucaille P. Molecular characterization and transcriptional analysis of the putative hydrogenase gene of Clostridium acetobutylicum ATCC 824. J Bacteriol. 1996;178:2668-75 pubmed
    A 2.8-kbp DNA region of Clostridium acetobutylicum ATCC 824 containing the putative hydrogenase gene (hydA) was cloned and sequenced...
  4. Kutty R, Bennett G. Biochemical characterization of trinitrotoluene transforming oxygen-insensitive nitroreductases from Clostridium acetobutylicum ATCC 824. Arch Microbiol. 2005;184:158-67 pubmed publisher
    ..Both the nitroreductases were inhibited by dicoumarol and salicyl hydroxamate. The nitroreductases showed higher relative expression on induction with TNT, nitrofurazone and nitrofurantoin compared to the uninduced control...
  5. Belouski E, Watson D, Bennett G. Cloning, sequence, and expression of the phosphofructokinase gene of Clostridium acetobutylicum ATCC 824 in Escherichia coli. Curr Microbiol. 1998;37:17-22 pubmed
    The pfk gene encoding phosphofructokinase (Pfk) from the anaerobic bacterium Clostridium acetobutylicum ATCC 824 was cloned and sequenced. The gene was identified in a plasmid library by complementation of an E...
  6. Jones S, Tracy B, Gaida S, Papoutsakis E. Inactivation of ?F in Clostridium acetobutylicum ATCC 824 blocks sporulation prior to asymmetric division and abolishes ?E and ?G protein expression but does not block solvent formation. J Bacteriol. 2011;193:2429-40 pubmed publisher
    ..acetobutylicum chromosome--with the aim of altering cell metabolism and differentiation...
  7. Nair R, Bennett G, Papoutsakis E. Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824. J Bacteriol. 1994;176:871-85 pubmed
    ..Petersen, E. T. Papoutsakis, and G. N. Bennett, Appl. Environ. Microbiol. 56:1576-1583, 1990) of Clostridium acetobutylicum ATCC 824 and sequenced. The 2,619-bp aad codes for a 96,517-Da protein...
  8. Walter K, Nair R, Cary J, Bennett G, Papoutsakis E. Sequence and arrangement of two genes of the butyrate-synthesis pathway of Clostridium acetobutylicum ATCC 824. Gene. 1993;134:107-11 pubmed
    The genes encoding both Clostridium acetobutylicum ATCC 824 butyrate synthesis pathway enzymes, phosphotransbutyrylase (ptb) and butyrate kinase (buk), were sequenced...
  9. Bi C, Jones S, Hess D, Tracy B, Papoutsakis E. SpoIIE is necessary for asymmetric division, sporulation, and expression of sigmaF, sigmaE, and sigmaG but does not control solvent production in Clostridium acetobutylicum ATCC 824. J Bacteriol. 2011;193:5130-7 pubmed publisher
    ..Thus, we concluded that SpoIIE does not control solvent formation...
  10. Nair R, Green E, Watson D, Bennett G, Papoutsakis E. Regulation of the sol locus genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 by a putative transcriptional repressor. J Bacteriol. 1999;181:319-30 pubmed
    ..a repressor of the sol locus (aad, ctfA, ctfB and adc) genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824. Primer extension analysis identified a transcriptional start site 35 bp upstream of the solR start ..
  11. Stim Herndon K, Petersen D, Bennett G. Characterization of an acetyl-CoA C-acetyltransferase (thiolase) gene from Clostridium acetobutylicum ATCC 824. Gene. 1995;154:81-5 pubmed
    ..either acids (acetate or butyrate) or solvents (acetone, butanol or ethanol) during the growth of Clostridium acetobutylicum ATCC 824. Cloning and expression of the Thl-encoding gene (thl) has been described [Petersen and Bennett, ..
  12. Huang K, Huang S, Rudolph F, Bennett G. Identification and characterization of a second butyrate kinase from Clostridium acetobutylicum ATCC 824. J Mol Microbiol Biotechnol. 2000;2:33-8 pubmed
    ..The expression of the BKII in Clostridium acetobutylicum ATCC 824 was further examined by Western blot analysis using a polyclonal antibody prepared against ..
  13. Cary J, Petersen D, Papoutsakis E, Bennett G. Cloning and expression of Clostridium acetobutylicum ATCC 824 acetoacetyl-coenzyme A:acetate/butyrate:coenzyme A-transferase in Escherichia coli. Appl Environ Microbiol. 1990;56:1576-83 pubmed
    ..8.3.9]) of Clostridium acetobutylicum ATCC 824 is an important enzyme in the metabolic shift between the acid-producing and solvent-forming states ..
  14. Tangney M, Mitchell W. Analysis of a catabolic operon for sucrose transport and metabolism in Clostridium acetobutylicum ATCC 824. J Mol Microbiol Biotechnol. 2000;2:71-80 pubmed
    The utilization of sucrose by Clostridium acetobutylicum ATCC 824 was investigated...
  15. Scotcher M, Bennett G. SpoIIE regulates sporulation but does not directly affect solventogenesis in Clostridium acetobutylicum ATCC 824. J Bacteriol. 2005;187:1930-6 pubmed publisher
    ..vectors, we examined the activity of the spoIIE promoter in wild-type and spo0A-deleted strains of Clostridium acetobutylicum ATCC 824. In wild-type cells, the spoIIE promoter is active in a transient manner during late ..
  16. Fontaine L, Meynial Salles I, Girbal L, Yang X, Croux C, Soucaille P. Molecular characterization and transcriptional analysis of adhE2, the gene encoding the NADH-dependent aldehyde/alcohol dehydrogenase responsible for butanol production in alcohologenic cultures of Clostridium acetobutylicum ATCC 824. J Bacteriol. 2002;184:821-30 pubmed
    The adhE2 gene of Clostridium acetobutylicum ATCC 824, coding for an aldehyde/alcohol dehydrogenase (AADH), was characterized from molecular and biochemical points of view. The 2,577-bp adhE2 codes for a 94.4-kDa protein...
  17. Sabath F, Croux C, Cornillot E, Soucaille P. amyP, a reporter gene to study strain degeneration in Clostridium acetobutylicum ATCC 824. FEMS Microbiol Lett. 2002;210:93-8 pubmed
    ..Degeneration of C. acetobutylicum has been associated to the loss of pSOL1. We demonstrate here that amyP can be used as a reporter system to quantitatively follow this phenomenon...
  18. Walter K, Bennett G, Papoutsakis E. Molecular characterization of two Clostridium acetobutylicum ATCC 824 butanol dehydrogenase isozyme genes. J Bacteriol. 1992;174:7149-58 pubmed
    ..The -10 and -35 promoter regions for these genes were almost identical. bdhA and bdhB were found to be induced or derepressed immediately prior to significant butanol production in controlled pH 5.0 batch fermentations...
  19. Tangney M, Galinier A, Deutscher J, Mitchell W. Analysis of the elements of catabolite repression in Clostridium acetobutylicum ATCC 824. J Mol Microbiol Biotechnol. 2003;6:6-11 pubmed publisher
    The PTSH gene, encoding the phosphotransferase protein HPr, from Clostridium acetobutylicum ATCC 824 was identified from the genome sequence, cloned and shown to complement a PTSH mutant of Escherichia coli...
  20. Ali M, Rudolph F, Bennett G. Characterization of thermostable Xyn10A enzyme from mesophilic Clostridium acetobutylicum ATCC 824. J Ind Microbiol Biotechnol. 2005;32:12-8 pubmed publisher
    A thermostable xylanase gene, xyn10A (CAP0053), was cloned from Clostridium acetobutylicum ATCC 824. The nucleotide sequence of the C...
  21. Schreiber W, Dürre P. The glyceraldehyde-3-phosphate dehydrogenase of Clostridium acetobutylicum: isolation and purification of the enzyme, and sequencing and localization of the gap gene within a cluster of other glycolytic genes. Microbiology. 1999;145 ( Pt 8):1839-47 pubmed
    ..No sequences resembling rho-independent transcription terminators were found in the intergenic regions. A plasmid carrying the clostridial gap gene complemented an Escherichia coli gap mutant. ..
  22. Highbarger L, Gerlt J, Kenyon G. Mechanism of the reaction catalyzed by acetoacetate decarboxylase. Importance of lysine 116 in determining the pKa of active-site lysine 115. Biochemistry. 1996;35:41-6 pubmed
    ..9. These data support Westheimer's hypothesis that the pKa of the Schiff base-forming Lys 115 is decreased by its spatial proximity to the epsilon-ammonium group of Lys 116. ..
  23. DiDonato M, Krishna S, Schwarzenbacher R, McMullan D, Agarwalla S, Brittain S, et al. Crystal structure of 2-phosphosulfolactate phosphatase (ComB) from Clostridium acetobutylicum at 2.6 A resolution reveals a new fold with a novel active site. Proteins. 2006;65:771-6 pubmed
  24. Sauer U, Dürre P. Sequence and molecular characterization of a DNA region encoding a small heat shock protein of Clostridium acetobutylicum. J Bacteriol. 1993;175:3394-400 pubmed
    ..A putative rho-independent transcription termination structure was identified at the 3' end of hsp18. ..
  25. Petersen D, Welch R, Walter K, Mermelstein L, Papoutsakis E, Rudolph F, et al. Cloning of an NADH-dependent butanol dehydrogenase gene from Clostridium acetobutylicum. Ann N Y Acad Sci. 1991;646:94-8 pubmed
    ..Maxicell analysis of [35S]-radiolabeled plasmid-encoded proteins identified a species encoded by the clostridial insert with the expected Mr of 42 kD. ..
  26. Sauer U, Dürre P. Possible function of tRNA(Thr)ACG in regulation of solvent formation in Clostridium acetobutylicum. FEMS Microbiol Lett. 1992;100:147-53 pubmed
    ..Evolutionary aspects of this codon selection and a possible translational regulation mechanism are discussed. ..
  27. Wong J, Sass C, Bennett G. Sequence and arrangement of genes encoding sigma factors in Clostridium acetobutylicum ATCC 824. Gene. 1995;153:89-92 pubmed
    The nucleotide sequence of a 2.7-kb region of Clostridium acetobutylicum ATCC 824 DNA containing three open reading frames was determined...
  28. Guedon E, Petitdemange H. Identification of the gene encoding NADH-rubredoxin oxidoreductase in Clostridium acetobutylicum. Biochem Biophys Res Commun. 2001;285:496-502 pubmed
  29. Germane K, Servinsky M, Gerlach E, Sund C, Hurley M. Structural analysis of Clostridium acetobutylicum ATCC 824 glycoside hydrolase from CAZy family GH105. Acta Crystallogr F Struct Biol Commun. 2015;71:1100-8 pubmed publisher
    b>Clostridium acetobutylicum ATCC 824 gene CA_C0359 encodes a putative unsaturated rhamnogalacturonyl hydrolase (URH) with distant amino-acid sequence homology to YteR of Bacillus subtilis strain 168...
  30. Morra S, Cordara A, Gilardi G, Valetti F. Atypical effect of temperature tuning on the insertion of the catalytic iron-sulfur center in a recombinant [FeFe]-hydrogenase. Protein Sci. 2015;24:2090-4 pubmed publisher
  31. Flengsrud R, Skjeldal L. Two-dimensional gel electrophoresis separation and N-terminal sequence analysis of proteins from Clostridium pasteurianum W5. Electrophoresis. 1998;19:802-6 pubmed
    ..Amino-terminal sequence determination and sequence analysis allowed the identification of 20 proteins, while 11 protein sequences remained unidentified and one protein appeared to have a blocked amino terminus. ..
  32. Externbrink T, Hujer S, Winzer K, Dürre P. Sequence analysis of the atp operon of Clostridium acetobutylicum DSM 792 encoding the F0F1 ATP synthase. DNA Seq. 2000;11:109-18 pubmed
    The atp gene region of Clostridium acetobutylicum DSM 792 has been fully sequenced...
  33. Laursen R, Westheimer F. The active site of cetoacetate decarboxylase. J Am Chem Soc. 1966;88:3426-30 pubmed
  34. Hu K, Zhao M, Zhang T, Zha M, Zhong C, Jiang Y, et al. Structures of trans-2-enoyl-CoA reductases from Clostridium acetobutylicum and Treponema denticola: insights into the substrate specificity and the catalytic mechanism. Biochem J. 2013;449:79-89 pubmed publisher
    ..A modelling study shows that the hydrophobic channel is optimal in both width and length for the binding of crotonyl-CoA. These results provide molecular bases for the high substrate specificity and the catalytic mechanism of TERs. ..
  35. Petersen D, Bennett G. Cloning of the Clostridium acetobutylicum ATCC 824 acetyl coenzyme A acetyltransferase (thiolase; EC 2.3.1.9) gene. Appl Environ Microbiol. 1991;57:2735-41 pubmed
  36. Nguyen N, Linder S, Flitsch S, Schiel Bengelsdorf B, Dürre P, Soucaille P. Cap0037, a Novel Global Regulator of Clostridium acetobutylicum Metabolism. MBio. 2016;7: pubmed publisher
    ..This is pioneering work for further determining the regulatory mechanism of Cap0037 in C. acetobutylicum and studying the role of proteins homologous to Cap0037 in other members of the phylum Firmicutes. ..
  37. Reith J, Berking A, Mayer C. Characterization of an N-acetylmuramic acid/N-acetylglucosamine kinase of Clostridium acetobutylicum. J Bacteriol. 2011;193:5386-92 pubmed publisher
    ..We propose a role of MurK in the recovery of muropeptides during cell wall rescue in C. acetobutylicum. The kinase was applied for high-sensitive detection of the amino sugars in cell wall preparations by radioactive phosphorylation. ..
  38. Sund C, Liu S, Germane K, Servinsky M, Gerlach E, Hurley M. Phosphoketolase flux in Clostridium acetobutylicum during growth on L-arabinose. Microbiology. 2015;161:430-40 pubmed publisher
    ..Greater flux through the PKP during growth on arabinose when compared with xylose indicated the pathway's primary role in C. acetobutylicum is arabinose metabolism. ..
  39. Narberhaus F, Bahl H. Cloning, sequencing, and molecular analysis of the groESL operon of Clostridium acetobutylicum. J Bacteriol. 1992;174:3282-9 pubmed
  40. Joshi N, Shepard E, Byer A, Swanson K, Broderick J, Peters J. Iron-sulfur cluster coordination in the [FeFe]-hydrogenase H cluster biosynthetic factor HydF. FEBS Lett. 2012;586:3939-43 pubmed publisher
    ..The results implicate a potential role for these residues in biosynthesis specifically and potential in bridging the [4Fe-4S] cluster to 2Fe subcluster biosynthetic intermediates. ..
  41. Malca S, Girhard M, Schuster S, D rre P, Urlacher V. Expression, purification and characterization of two Clostridium acetobutylicum flavodoxins: potential electron transfer partners for CYP152A2. Biochim Biophys Acta. 2011;1814:257-64 pubmed publisher
    ..acetobutylicum. Flavodoxin CacFld2 did not accept electrons from NADPH-reduced FdR, though it cannot be excluded as a candidate redox partner for CYP152A2 in the presence of an appropriate physiological reductase...
  42. Steiner E, Scott J, Minton N, Winzer K. An agr quorum sensing system that regulates granulose formation and sporulation in Clostridium acetobutylicum. Appl Environ Microbiol. 2012;78:1113-22 pubmed publisher
    ..Together, these findings support the hypothesis that agr-dependent quorum sensing is involved in the regulation of sporulation and granulose formation in C. acetobutylicum...
  43. D rre P, Fischer R, Kuhn A, Lorenz K, Schreiber W, St rzenhofecker B, et al. Solventogenic enzymes of Clostridium acetobutylicum: catalytic properties, genetic organization, and transcriptional regulation. FEMS Microbiol Rev. 1995;17:251-62 pubmed
    ..Although the signal that triggers the shift to solventogenesis has not yet been elucidated, recent investigations suggest a possible function of DNA supercoiling as a transcriptional sensor of the respective environmental stimuli...
  44. Sauer U, Treuner A, Buchholz M, Santangelo J, D rre P. Sporulation and primary sigma factor homologous genes in Clostridium acetobutylicum. J Bacteriol. 1994;176:6572-82 pubmed
    Using a PCR-based approach, we have cloned various sigma factor homologous genes from Clostridium acetobutylicum DSM 792. The nucleotide sequence of the dnaE-sigA operon has been determined and predicts two genes encoding 69- and 43-kDa ..
  45. Fiedler T, Mix M, Meyer U, Mikkat S, Glocker M, Bahl H, et al. The two-component system PhoPR of Clostridium acetobutylicum is involved in phosphate-dependent gene regulation. J Bacteriol. 2008;190:6559-67 pubmed publisher
    The phoPR gene locus of Clostridium acetobutylicum ATCC 824 comprises two genes, phoP and phoR. Deduced proteins are predicted to represent a response regulator and sensor kinase of a phosphate-dependent two-component regulatory system...
  46. Demuez M, Cournac L, Guerrini O, Soucaille P, Girbal L. Complete activity profile of Clostridium acetobutylicum [FeFe]-hydrogenase and kinetic parameters for endogenous redox partners. FEMS Microbiol Lett. 2007;275:113-21 pubmed publisher
    ..The kinetic parameters of C. acetobutylicum [FeFe]-hydrogenase for both physiological partners, ferredoxin CAC0303 and flavodoxin CAC0587, are reported for hydrogen uptake and hydrogen evolution activities...
  47. Petersen D, Cary J, Vanderleyden J, Bennett G. Sequence and arrangement of genes encoding enzymes of the acetone-production pathway of Clostridium acetobutylicum ATCC824. Gene. 1993;123:93-7 pubmed
    ..The intervening DNA region separating the two transcripts is characterized by an inverted repeat which appears capable of forming a stem-loop structure functioning as a Rho-independent transcription terminator in both directions...
  48. Croux C, Garc a J. Sequence of the lyc gene encoding the autolytic lysozyme of Clostridium acetobutylicum ATCC824: comparison with other lytic enzymes. Gene. 1991;104:25-31 pubmed
    ..The existence of five putative repeated motifs in the C-terminal region of the autolytic lysozyme suggests that this region could play a role in the recognition of the polymeric substrate...
  49. Al Hinai M, Jones S, Papoutsakis E. ?K of Clostridium acetobutylicum is the first known sporulation-specific sigma factor with two developmentally separated roles, one early and one late in sporulation. J Bacteriol. 2014;196:287-99 pubmed publisher
    ..This is the first sporulation-specific sigma factor shown to have two developmentally separated roles...
  50. Gerischer U, D rre P. Cloning, sequencing, and molecular analysis of the acetoacetate decarboxylase gene region from Clostridium acetobutylicum. J Bacteriol. 1990;172:6907-18 pubmed
    Acetoacetate decarboxylase (ADC) (EC4.1.1.4) of Clostridium acetobutylicum DSM 792 was purified to homogeneity, and its first 25 N-terminal amino acids were determined...
  51. Winzer K, Lorenz K, Zickner B, D rre P. Differential regulation of two thiolase genes from Clostridium acetobutylicum DSM 792. J Mol Microbiol Biotechnol. 2000;2:531-41 pubmed
    ..Two thiolase genes (thlA and thIB) have been cloned and sequenced from Clostridium acetobutylicum DSM 792, showing high homology to each other and to thiolases of PHA-synthesizing bacteria...
  52. Narberhaus F, Giebeler K, Bahl H. Molecular characterization of the dnaK gene region of Clostridium acetobutylicum, including grpE, dnaJ, and a new heat shock gene. J Bacteriol. 1992;174:3290-9 pubmed
    ..Maximum mRNA levels of this heat shock operon were found about 15 min after a heat shock from 30 to 42 degrees C. Our results indicate that orfA codes for an unknown heat shock protein...
  53. Santangelo J, Kuhn A, Treuner Lange A, D rre P. Sporulation and time course expression of sigma-factor homologous genes in Clostridium acetobutylicum. FEMS Microbiol Lett. 1998;161:157-64 pubmed
    ..From primer extension experiments promoter structures could be deduced with high homology to the Bacillus consensus. Upstream of the spoIIGA-sigE operon a gene with significant similarity to ftsZ could be detected...
  54. Suttisansanee U, Lau K, Lagishetty S, Rao K, Swaminathan S, Sauder J, et al. Structural variation in bacterial glyoxalase I enzymes: investigation of the metalloenzyme glyoxalase I from Clostridium acetobutylicum. J Biol Chem. 2011;286:38367-74 pubmed publisher
    ..These findings indicate that metal activation profiles in this class of enzyme hold true across diverse quaternary structure arrangements...
  55. Riebe O, Fischer R, Bahl H. Desulfoferrodoxin of Clostridium acetobutylicum functions as a superoxide reductase. FEBS Lett. 2007;581:5605-10 pubmed publisher
    ..Thus, we assume that desulfoferrodoxin is the key factor in the superoxide reductase dependent part of an alternative pathway for detoxification of reactive oxygen species in this obligate anaerobic bacterium...
  56. Steiner E, Dago A, Young D, Heap J, Minton N, Hoch J, et al. Multiple orphan histidine kinases interact directly with Spo0A to control the initiation of endospore formation in Clostridium acetobutylicum. Mol Microbiol. 2011;80:641-54 pubmed publisher
    ..4 billion years ago, after which additional phosphorelay proteins were recruited in the evolutionary lineage that led to the bacilli...
  57. Gerischer U, D rre P. mRNA analysis of the adc gene region of Clostridium acetobutylicum during the shift to solventogenesis. J Bacteriol. 1992;174:426-33 pubmed
    ..Gerischer and P. Dürre, J. Bacteriol. 172:6907-6918, 1990), probably is indeed a coding region. ORF2, however, does not seem to have a coding function...
  58. Faucher F, Wallace S, Doubli S. Structural basis for the lack of opposite base specificity of Clostridium acetobutylicum 8-oxoguanine DNA glycosylase. DNA Repair (Amst). 2009;8:1283-9 pubmed publisher
    ..A structural comparison with human OGG1 provides a rationale for the lack of opposite base specificity displayed by the bacterial Ogg...
  59. Zhu L, Cheng J, Luo B, Feng S, Lin J, Wang S, et al. Functions of the Clostridium acetobutylicium FabF and FabZ proteins in unsaturated fatty acid biosynthesis. BMC Microbiol. 2009;9:119 pubmed publisher
    ..coli unsaturated fatty acid biosynthesis in vivo and lacked isomerase activity in vitro. Thus, C. acetobutylicium introduces the double of unsaturated fatty acids by use of a novel and unknown enzyme...
  60. Faucher F, Robey Bond S, Wallace S, Doubli S. Structural characterization of Clostridium acetobutylicum 8-oxoguanine DNA glycosylase in its apo form and in complex with 8-oxodeoxyguanosine. J Mol Biol. 2009;387:669-79 pubmed publisher
    ..A structural comparison of CacOgg with human OGG1, in complex with 8-oxoG containing DNA, provides a structural rationale for the lack of opposite base specificity displayed by CacOgg...
  61. Girhard M, Schuster S, Dietrich M, D rre P, Urlacher V. Cytochrome P450 monooxygenase from Clostridium acetobutylicum: a new alpha-fatty acid hydroxylase. Biochem Biophys Res Commun. 2007;362:114-9 pubmed publisher
    ..coli and even 30-40 times more substrate with the CYP102A1-reductase from Bacillus megaterium. According to the clear preference for hydroxylation at alpha-position, CYP152A2 can be referred to as fatty acid alpha-hydroxylase...
  62. Robey Bond S, Barrantes Reynolds R, Bond J, Wallace S, Bandaru V. Clostridium acetobutylicum 8-oxoguanine DNA glycosylase (Ogg) differs from eukaryotic Oggs with respect to opposite base discrimination. Biochemistry. 2008;47:7626-36 pubmed publisher
  63. Behrens S, Mitchell W, Bahl H. Molecular analysis of the mannitol operon of Clostridium acetobutylicum encoding a phosphotransferase system and a putative PTS-modulated regulator. Microbiology. 2001;147:75-86 pubmed publisher
    b>Clostridium acetobutylicum DSM 792 accumulates and phosphorylates mannitol via a phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS)...
  64. Treuner Lange A, Kuhn A, D rre P. The kdp system of Clostridium acetobutylicum: cloning, sequencing, and transcriptional regulation in response to potassium concentration. J Bacteriol. 1997;179:4501-12 pubmed
    ..Except for the two small peptides, all gene products could be detected in in vitro transcription-translation experiments...
  65. Wietzke M, Bahl H. The redox-sensing protein Rex, a transcriptional regulator of solventogenesis in Clostridium acetobutylicum. Appl Microbiol Biotechnol. 2012;96:749-61 pubmed publisher
    ..The results presented here clearly indicated that Rex is involved in the redox-dependent solventogenic shift of C. acetobutylicum...
  66. Guerrini O, Burlat B, L ger C, Guigliarelli B, Soucaille P, Girbal L. Characterization of two 2[4Fe4S] ferredoxins from Clostridium acetobutylicum. Curr Microbiol. 2008;56:261-7 pubmed publisher
    ..The physiological function of CAC3527 is discussed...
  67. Valvano M, Messner P, Kosma P. Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides. Microbiology. 2002;148:1979-89 pubmed publisher