Clostridium acetobutylicum ATCC 824

Summary

Alias: Clostridium acetobutylicum DSM 792, Clostridium acetobutylicum str. ATCC 824, Clostridium acetobutylicum strain ATCC 824

Top Publications

  1. pmc Cloning, sequencing, and expression of genes encoding phosphotransacetylase and acetate kinase from Clostridium acetobutylicum ATCC 824
    Z L Boynton
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005, USA
    Appl Environ Microbiol 62:2758-66. 1996
  2. ncbi Biochemical characterization of trinitrotoluene transforming oxygen-insensitive nitroreductases from Clostridium acetobutylicum ATCC 824
    Razia Kutty
    Department of Biochemistry and Cell Biology MS 140, Rice University, Houston, TX 77005 1892, USA
    Arch Microbiol 184:158-67. 2005
  3. ncbi Characterization of thermostable Xyn10A enzyme from mesophilic Clostridium acetobutylicum ATCC 824
    Mursheda K Ali
    Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005, USA
    J Ind Microbiol Biotechnol 32:12-8. 2005
  4. ncbi Analysis of the elements of catabolite repression in Clostridium acetobutylicum ATCC 824
    Martin Tangney
    School of Life Sciences, Napier University, Edinburgh, UK
    J Mol Microbiol Biotechnol 6:6-11. 2003
  5. ncbi Genes malh and pagl of Clostridium acetobutylicum ATCC 824 encode NAD+- and Mn2+-dependent phospho-alpha-glucosidase(s)
    John Thompson
    Microbial Biochemistry and Genetics Unit, Oral Infection and Immunity Branch, NIDCR, NIH, Bethesda, MD 20892, USA
    J Biol Chem 279:1553-61. 2004
  6. ncbi amyP, a reporter gene to study strain degeneration in Clostridium acetobutylicum ATCC 824
    Fabrice Sabathé
    Centre de Bioingenierie Gilbert Durand, UMR CNRS 5504, Lab Ass INRA, Institut National des Sciences Appliquees, 135 Avenue de Rangueil, 31077 Toulouse, France
    FEMS Microbiol Lett 210:93-8. 2002
  7. pmc Molecular characterization and transcriptional analysis of adhE2, the gene encoding the NADH-dependent aldehyde/alcohol dehydrogenase responsible for butanol production in alcohologenic cultures of Clostridium acetobutylicum ATCC 824
    Lisa Fontaine
    Centre de Bioingenierie Gilbert Durand, Laboratoire de Biotechnologies Bioprocédés, UMR CNRS 5504, UR INRA 792, INSA, 31077 Toulouse Cedex 4, France
    J Bacteriol 184:821-30. 2002
  8. pmc Regulation of the sol locus genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 by a putative transcriptional repressor
    R V Nair
    Department of Chemical Engineering, Northwestern University, Evanston, Illinois 60208, USA
    J Bacteriol 181:319-30. 1999
  9. ncbi Cloning, sequence, and expression of the phosphofructokinase gene of Clostridium acetobutylicum ATCC 824 in Escherichia coli
    E Belouski
    Department of Biochemistry and Cell Biology MS 140, 6100 Main St, Rice University, Houston, TX 77005 1892, USA
    Curr Microbiol 37:17-22. 1998
  10. pmc Molecular characterization and transcriptional analysis of the putative hydrogenase gene of Clostridium acetobutylicum ATCC 824
    M F Gorwa
    Institut National des Sciences Appliquees, Centre de Bioingénierie G Durand, Toulouse, France
    J Bacteriol 178:2668-75. 1996

Patents

  1. Plants with increased yield (lt)
  2. Process for the production of a fine chemical
  3. Proteins associated with abiotic stress response and homologs
  4. Process for producing peptide
  5. RECOMBINANT HOST CELLS COMPRISING PHOSPHOKETOLASES
  6. HIGHLY CONSERVED GENES AND THEIR USE TO GENERATE SPECIES-SPECIFIC, GENUS-SPECIFIC, FAMILY-SPECIFIC, GROUP-SPECIFIC AND UNIVERSAL NUCLEIC ACID PROBES AND AMPLIFICATION PRIMERS TO RAPIDLY DETECT AND IDENTIFY ALGAL, ARCHAEAL, BACTERIAL, FUNGAL AND PARASITICAL MICROOR-GANISMS FROM CLINICAL SPECIMENS FOR DIAGNOSIS
  7. Proteins associated with abiotic stress response and homologs
  8. Process for the control of production of fine chemicals
  9. Process for the production of fine chemicals
  10. Plants with increased yield (nue)

Scientific Experts

  • M A Valvano
  • John Thompson
  • Klaus Winzer
  • M Tangney
  • R Flengsrud
  • Lei Zhu
  • Simone Morra
  • Shawn W Jones
  • Elisabeth Steiner
  • Frédérick Faucher
  • Katherine L Germane
  • Christian J Sund
  • G N Bennett
  • Eleftherios T Papoutsakis
  • Philippe Soucaille
  • Hubert Bahl
  • Susan S Wallace
  • P Dürre
  • Susan M Robey-Bond
  • Marco Girhard
  • Olivier Guerrini
  • Matthew D Servinsky
  • Elliot S Gerlach
  • Margaret M Hurley
  • Mohab A Al-Hinai
  • Laurence Girbal
  • Gianfranco Gilardi
  • Francesca Valetti
  • Kuan Hu
  • George N Bennett
  • Neelambari Joshi
  • Mandy Wietzke
  • Nigel P Minton
  • Changhao Bi
  • Bryan P Tracy
  • Sumire Honda Malca
  • Uthaiwan Suttisansanee
  • Jan Reith
  • SYLVIE DOUBLIE
  • Peter Dürre
  • Vlada B Urlacher
  • Stefanie Schuster
  • Tomas Fiedler
  • Ralf Jörg Fischer
  • Oliver Riebe
  • Marie Demuez
  • Michael DiDonato
  • E Belouski
  • Mursheda K Ali
  • Razia Kutty
  • Miles C Scotcher
  • R V Nair
  • S Behrens
  • Alessandro Cordara
  • Sanchao Liu
  • F B Rudolph
  • Lisa Fontaine
  • Christian Croux
  • Fabrice Sabathé
  • Tianlong Zhang
  • Meng Zhao
  • Chen Zhong
  • Manwu Zha
  • Yu Jiang
  • W Schreiber
  • U Sauer
  • Jianping Ding
  • Giovanna Di Nardo
  • Amanda S Byer
  • Kevin D Swanson
  • Joan B Broderick
  • Paul W King
  • E Guedon
  • Jamie Scott
  • John W Peters
  • A Treuner-Lange
  • Eric M Shepard
  • Alberto Giraudo
  • Christoph Mayer
  • Stephen K Burley
  • Michael Young
  • Stefan M Gaida
  • J Michael Sauder
  • James A Hoch
  • John T Heap
  • Krishnamurthy N Rao
  • John F Honek
  • Daniel R Hess
  • Kelvin Lau
  • Anne Berking

Detail Information

Publications70

  1. pmc Cloning, sequencing, and expression of genes encoding phosphotransacetylase and acetate kinase from Clostridium acetobutylicum ATCC 824
    Z L Boynton
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005, USA
    Appl Environ Microbiol 62:2758-66. 1996
    ..The results from overexpression of ack and pta in C. acetobutylicum showed that the final ratios of acetate to other major products were higher and that there was a greater proportion of two- versus four-carbon-derived products...
  2. ncbi Biochemical characterization of trinitrotoluene transforming oxygen-insensitive nitroreductases from Clostridium acetobutylicum ATCC 824
    Razia Kutty
    Department of Biochemistry and Cell Biology MS 140, Rice University, Houston, TX 77005 1892, USA
    Arch Microbiol 184:158-67. 2005
    ..Both the nitroreductases were inhibited by dicoumarol and salicyl hydroxamate. The nitroreductases showed higher relative expression on induction with TNT, nitrofurazone and nitrofurantoin compared to the uninduced control...
  3. ncbi Characterization of thermostable Xyn10A enzyme from mesophilic Clostridium acetobutylicum ATCC 824
    Mursheda K Ali
    Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005, USA
    J Ind Microbiol Biotechnol 32:12-8. 2005
    A thermostable xylanase gene, xyn10A (CAP0053), was cloned from Clostridium acetobutylicum ATCC 824. The nucleotide sequence of the C...
  4. ncbi Analysis of the elements of catabolite repression in Clostridium acetobutylicum ATCC 824
    Martin Tangney
    School of Life Sciences, Napier University, Edinburgh, UK
    J Mol Microbiol Biotechnol 6:6-11. 2003
    The PTSH gene, encoding the phosphotransferase protein HPr, from Clostridium acetobutylicum ATCC 824 was identified from the genome sequence, cloned and shown to complement a PTSH mutant of Escherichia coli...
  5. ncbi Genes malh and pagl of Clostridium acetobutylicum ATCC 824 encode NAD+- and Mn2+-dependent phospho-alpha-glucosidase(s)
    John Thompson
    Microbial Biochemistry and Genetics Unit, Oral Infection and Immunity Branch, NIDCR, NIH, Bethesda, MD 20892, USA
    J Biol Chem 279:1553-61. 2004
    ..Of the two putative operons, our findings suggest that only proteins encoded by the mal operon participate in the dissimilation of maltose and related O-alpha-linked glucosides by C. acetobutylicum 824...
  6. ncbi amyP, a reporter gene to study strain degeneration in Clostridium acetobutylicum ATCC 824
    Fabrice Sabathé
    Centre de Bioingenierie Gilbert Durand, UMR CNRS 5504, Lab Ass INRA, Institut National des Sciences Appliquees, 135 Avenue de Rangueil, 31077 Toulouse, France
    FEMS Microbiol Lett 210:93-8. 2002
    ..Degeneration of C. acetobutylicum has been associated to the loss of pSOL1. We demonstrate here that amyP can be used as a reporter system to quantitatively follow this phenomenon...
  7. pmc Molecular characterization and transcriptional analysis of adhE2, the gene encoding the NADH-dependent aldehyde/alcohol dehydrogenase responsible for butanol production in alcohologenic cultures of Clostridium acetobutylicum ATCC 824
    Lisa Fontaine
    Centre de Bioingenierie Gilbert Durand, Laboratoire de Biotechnologies Bioprocédés, UMR CNRS 5504, UR INRA 792, INSA, 31077 Toulouse Cedex 4, France
    J Bacteriol 184:821-30. 2002
    The adhE2 gene of Clostridium acetobutylicum ATCC 824, coding for an aldehyde/alcohol dehydrogenase (AADH), was characterized from molecular and biochemical points of view. The 2,577-bp adhE2 codes for a 94.4-kDa protein...
  8. pmc Regulation of the sol locus genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 by a putative transcriptional repressor
    R V Nair
    Department of Chemical Engineering, Northwestern University, Evanston, Illinois 60208, USA
    J Bacteriol 181:319-30. 1999
    ..a repressor of the sol locus (aad, ctfA, ctfB and adc) genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824. Primer extension analysis identified a transcriptional start site 35 bp upstream of the solR start ..
  9. ncbi Cloning, sequence, and expression of the phosphofructokinase gene of Clostridium acetobutylicum ATCC 824 in Escherichia coli
    E Belouski
    Department of Biochemistry and Cell Biology MS 140, 6100 Main St, Rice University, Houston, TX 77005 1892, USA
    Curr Microbiol 37:17-22. 1998
    The pfk gene encoding phosphofructokinase (Pfk) from the anaerobic bacterium Clostridium acetobutylicum ATCC 824 was cloned and sequenced. The gene was identified in a plasmid library by complementation of an E...
  10. pmc Molecular characterization and transcriptional analysis of the putative hydrogenase gene of Clostridium acetobutylicum ATCC 824
    M F Gorwa
    Institut National des Sciences Appliquees, Centre de Bioingénierie G Durand, Toulouse, France
    J Bacteriol 178:2668-75. 1996
    A 2.8-kbp DNA region of Clostridium acetobutylicum ATCC 824 containing the putative hydrogenase gene (hydA) was cloned and sequenced...
  11. pmc Inactivation of σF in Clostridium acetobutylicum ATCC 824 blocks sporulation prior to asymmetric division and abolishes σE and σG protein expression but does not block solvent formation
    Shawn W Jones
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, USA
    J Bacteriol 193:2429-40. 2011
    ..acetobutylicum chromosome--with the aim of altering cell metabolism and differentiation...
  12. pmc Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824
    R V Nair
    Department of Chemical Engineering, Northwestern University, Evanston, Illinois 60208
    J Bacteriol 176:871-85. 1994
    ..Petersen, E. T. Papoutsakis, and G. N. Bennett, Appl. Environ. Microbiol. 56:1576-1583, 1990) of Clostridium acetobutylicum ATCC 824 and sequenced. The 2,619-bp aad codes for a 96,517-Da protein...
  13. pmc SpoIIE is necessary for asymmetric division, sporulation, and expression of sigmaF, sigmaE, and sigmaG but does not control solvent production in Clostridium acetobutylicum ATCC 824
    Changhao Bi
    Molecular Biotechnology Laboratory, Department of Chemical Engineering, Delaware Biotechnology Institute, University of Delaware, 15 Innovation Way, Newark, DE 19711, USA
    J Bacteriol 193:5130-7. 2011
    ..Thus, we concluded that SpoIIE does not control solvent formation...
  14. pmc Molecular characterization of two Clostridium acetobutylicum ATCC 824 butanol dehydrogenase isozyme genes
    K A Walter
    Department of Chemical Engineering, Northwestern University, Evanston, Illinois 60208
    J Bacteriol 174:7149-58. 1992
    ..The -10 and -35 promoter regions for these genes were almost identical. bdhA and bdhB were found to be induced or derepressed immediately prior to significant butanol production in controlled pH 5.0 batch fermentations...
  15. pmc Cloning and expression of Clostridium acetobutylicum ATCC 824 acetoacetyl-coenzyme A:acetate/butyrate:coenzyme A-transferase in Escherichia coli
    J W Cary
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77251
    Appl Environ Microbiol 56:1576-83. 1990
    ..8.3.9]) of Clostridium acetobutylicum ATCC 824 is an important enzyme in the metabolic shift between the acid-producing and solvent-forming states ..
  16. ncbi Characterization of an acetyl-CoA C-acetyltransferase (thiolase) gene from Clostridium acetobutylicum ATCC 824
    K P Stim-Herndon
    Department of Biochemistry and Cell Biology MS140, Rice University, Houston, TX 77005 1892
    Gene 154:81-5. 1995
    ..either acids (acetate or butyrate) or solvents (acetone, butanol or ethanol) during the growth of Clostridium acetobutylicum ATCC 824. Cloning and expression of the Thl-encoding gene (thl) has been described [Petersen and Bennett, ..
  17. ncbi Sequence and arrangement of two genes of the butyrate-synthesis pathway of Clostridium acetobutylicum ATCC 824
    K A Walter
    Department of Chemical Engineering, Northwestern University, Evanston, IL 60208
    Gene 134:107-11. 1993
    The genes encoding both Clostridium acetobutylicum ATCC 824 butyrate synthesis pathway enzymes, phosphotransbutyrylase (ptb) and butyrate kinase (buk), were sequenced...
  18. pmc SpoIIE regulates sporulation but does not directly affect solventogenesis in Clostridium acetobutylicum ATCC 824
    Miles C Scotcher
    Department of Biochemistry and Cell Biology, Rice University, 6100 Main St, Houston, TX 77005, USA
    J Bacteriol 187:1930-6. 2005
    ..vectors, we examined the activity of the spoIIE promoter in wild-type and spo0A-deleted strains of Clostridium acetobutylicum ATCC 824. In wild-type cells, the spoIIE promoter is active in a transient manner during late ..
  19. ncbi Analysis of a catabolic operon for sucrose transport and metabolism in Clostridium acetobutylicum ATCC 824
    M Tangney
    Department of Biological Sciences, Heriot Watt University, Riccarton, Edinburgh, UK
    J Mol Microbiol Biotechnol 2:71-80. 2000
    The utilization of sucrose by Clostridium acetobutylicum ATCC 824 was investigated...
  20. ncbi Identification and characterization of a second butyrate kinase from Clostridium acetobutylicum ATCC 824
    K X Huang
    Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005 1892, USA
    J Mol Microbiol Biotechnol 2:33-8. 2000
    ..The expression of the BKII in Clostridium acetobutylicum ATCC 824 was further examined by Western blot analysis using a polyclonal antibody prepared against ..
  21. pmc Clostridium acetobutylicum 8-oxoguanine DNA glycosylase (Ogg) differs from eukaryotic Oggs with respect to opposite base discrimination
    Susan M Robey-Bond
    Department of Microbiology and Molecular Genetics, The Markey Center for Molecular Genetics, University of Vermont, Stafford Hall, 95 Carrigan Drive, Burlington, Vermont 05405 0068, USA
    Biochemistry 47:7626-36. 2008
    ....
  22. pmc Atypical effect of temperature tuning on the insertion of the catalytic iron-sulfur center in a recombinant [FeFe]-hydrogenase
    Simone Morra
    Department of Life Sciences and Systems Biology, University of Torino, Via Accademia Albertina 13, Torino, 10123, Italy
    Protein Sci 24:2090-4. 2015
    ....
  23. pmc Structural analysis of Clostridium acetobutylicum ATCC 824 glycoside hydrolase from CAZy family GH105
    Katherine L Germane
    Oak Ridge Associated Universities, 4692 Millennium Drive, Suite 101, Belcamp, MD 21017, USA
    Acta Crystallogr F Struct Biol Commun 71:1100-8. 2015
    b>Clostridium acetobutylicum ATCC 824 gene CA_C0359 encodes a putative unsaturated rhamnogalacturonyl hydrolase (URH) with distant amino-acid sequence homology to YteR of Bacillus subtilis strain 168...
  24. doi Phosphoketolase flux in Clostridium acetobutylicum during growth on L-arabinose
    Christian J Sund
    US Army Research Laboratory, Sensors and Electron Devices Directorate, 2800 Powder Mill Road, Adelphi, MD 20783, USA
    Microbiology 161:430-40. 2015
    ..Greater flux through the PKP during growth on arabinose when compared with xylose indicated the pathway's primary role in C. acetobutylicum is arabinose metabolism. ..
  25. ncbi Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides
    Miguel A Valvano
    Department of Microbiology and Immunology and Medicine, University of Western Ontario, London, Ontario, N6A 5C1, Canada
    Microbiology 148:1979-89. 2002
  26. pmc σK of Clostridium acetobutylicum is the first known sporulation-specific sigma factor with two developmentally separated roles, one early and one late in sporulation
    Mohab A Al-Hinai
    Department of Biological Sciences, University of Delaware, Newark, Delaware, USA
    J Bacteriol 196:287-99. 2014
    ..This is the first sporulation-specific sigma factor shown to have two developmentally separated roles. ..
  27. ncbi Complete activity profile of Clostridium acetobutylicum [FeFe]-hydrogenase and kinetic parameters for endogenous redox partners
    Marie Demuez
    Laboratoire d Ingénierie des Systèmes Biologiques et des Procédés, UMR CNRS 5504, UMR INRA 792, INSA, Toulouse, France
    FEMS Microbiol Lett 275:113-21. 2007
    ..The kinetic parameters of C. acetobutylicum [FeFe]-hydrogenase for both physiological partners, ferredoxin CAC0303 and flavodoxin CAC0587, are reported for hydrogen uptake and hydrogen evolution activities...
  28. ncbi Cytochrome P450 monooxygenase from Clostridium acetobutylicum: a new alpha-fatty acid hydroxylase
    Marco Girhard
    Institute of Technical Biochemistry, University of Stuttgart, 70569 Stuttgart, Germany
    Biochem Biophys Res Commun 362:114-9. 2007
    ..coli and even 30-40 times more substrate with the CYP102A1-reductase from Bacillus megaterium. According to the clear preference for hydroxylation at alpha-position, CYP152A2 can be referred to as fatty acid alpha-hydroxylase...
  29. ncbi Crystal structure of 2-phosphosulfolactate phosphatase (ComB) from Clostridium acetobutylicum at 2.6 A resolution reveals a new fold with a novel active site
    Michael DiDonato
    The Joint Center for Structural Genomics
    Proteins 65:771-6. 2006
  30. pmc Characterization of an N-acetylmuramic acid/N-acetylglucosamine kinase of Clostridium acetobutylicum
    Jan Reith
    Fachbereich Biologie, Molekulare Mikrobiologie, Universitat Konstanz, 78457 Constance, Germany
    J Bacteriol 193:5386-92. 2011
    ..We propose a role of MurK in the recovery of muropeptides during cell wall rescue in C. acetobutylicum. The kinase was applied for high-sensitive detection of the amino sugars in cell wall preparations by radioactive phosphorylation...
  31. pmc Structural variation in bacterial glyoxalase I enzymes: investigation of the metalloenzyme glyoxalase I from Clostridium acetobutylicum
    Uthaiwan Suttisansanee
    Department of Chemistry, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada
    J Biol Chem 286:38367-74. 2011
    ..These findings indicate that metal activation profiles in this class of enzyme hold true across diverse quaternary structure arrangements...
  32. pmc An agr quorum sensing system that regulates granulose formation and sporulation in Clostridium acetobutylicum
    Elisabeth Steiner
    Address correspondence to Klaus Winzer, or Nigel P Minton
    Appl Environ Microbiol 78:1113-22. 2012
    ..Together, these findings support the hypothesis that agr-dependent quorum sensing is involved in the regulation of sporulation and granulose formation in C. acetobutylicum...
  33. doi The redox-sensing protein Rex, a transcriptional regulator of solventogenesis in Clostridium acetobutylicum
    Mandy Wietzke
    Abteilung Mikrobiologie, Institut für Biowissenschaften, Universitat Rostock, Albert Einstein Str 3, 18051, Rostock, Germany
    Appl Microbiol Biotechnol 96:749-61. 2012
    ..The results presented here clearly indicated that Rex is involved in the redox-dependent solventogenic shift of C. acetobutylicum...
  34. pmc Multiple orphan histidine kinases interact directly with Spo0A to control the initiation of endospore formation in Clostridium acetobutylicum
    Elisabeth Steiner
    Institute of Biological Environmental and Rural Sciences, Aberystwyth University, Ceredigion SY23 3DD, UK
    Mol Microbiol 80:641-54. 2011
    ..4 billion years ago, after which additional phosphorelay proteins were recruited in the evolutionary lineage that led to the bacilli...
  35. doi Expression, purification and characterization of two Clostridium acetobutylicum flavodoxins: potential electron transfer partners for CYP152A2
    Sumire Honda Malca
    Institute of Technical Biochemistry, Universitat Stuttgart, 70569 Stuttgart, Germany
    Biochim Biophys Acta 1814:257-64. 2011
    ..acetobutylicum. Flavodoxin CacFld2 did not accept electrons from NADPH-reduced FdR, though it cannot be excluded as a candidate redox partner for CYP152A2 in the presence of an appropriate physiological reductase...
  36. ncbi Identification of the gene encoding NADH-rubredoxin oxidoreductase in Clostridium acetobutylicum
    E Guedon
    Laboratoire de Biochimie des Bactéries Gram, Universite Henri Poincare, Faculte des Sciences, BP 239, 54506 Vandoeuvre les Nancy Cedex, France
    Biochem Biophys Res Commun 285:496-502. 2001
    ....
  37. pmc Site saturation mutagenesis demonstrates a central role for cysteine 298 as proton donor to the catalytic site in CaHydA [FeFe]-hydrogenase
    Simone Morra
    Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy
    PLoS ONE 7:e48400. 2012
    ....
  38. doi Structures of trans-2-enoyl-CoA reductases from Clostridium acetobutylicum and Treponema denticola: insights into the substrate specificity and the catalytic mechanism
    Kuan Hu
    State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China
    Biochem J 449:79-89. 2013
    ..A modelling study shows that the hydrophobic channel is optimal in both width and length for the binding of crotonyl-CoA. These results provide molecular bases for the high substrate specificity and the catalytic mechanism of TERs...
  39. pmc Structural basis for the lack of opposite base specificity of Clostridium acetobutylicum 8-oxoguanine DNA glycosylase
    Frédérick Faucher
    Department of Microbiology and Molecular Genetics, The Markey Center for Molecular Genetics, University of Vermont, Stafford Hall, 95 Carrigan Drive, Burlington, VT 05405 0068, USA
    DNA Repair (Amst) 8:1283-9. 2009
    ..A structural comparison with human OGG1 provides a rationale for the lack of opposite base specificity displayed by the bacterial Ogg...
  40. pmc Functions of the Clostridium acetobutylicium FabF and FabZ proteins in unsaturated fatty acid biosynthesis
    Lei Zhu
    College of Life Science, South China Agricultural University, Guangzhou 510642, PR China
    BMC Microbiol 9:119. 2009
    ..acetobutylicium, FabZ and FabF might also function in the unsaturated arm of the pathway (a FabZ homologue is known to be an unsaturated fatty acid synthetic enzyme in enterococci)...
  41. ncbi Desulfoferrodoxin of Clostridium acetobutylicum functions as a superoxide reductase
    Oliver Riebe
    University of Rostock, Institute of Biological Sciences, Division of Microbiology, Albert Einstein Strasse 3, D 18051, Rostock, Germany
    FEBS Lett 581:5605-10. 2007
    ..Thus, we assume that desulfoferrodoxin is the key factor in the superoxide reductase dependent part of an alternative pathway for detoxification of reactive oxygen species in this obligate anaerobic bacterium...
  42. pmc Structural characterization of Clostridium acetobutylicum 8-oxoguanine DNA glycosylase in its apo form and in complex with 8-oxodeoxyguanosine
    Frédérick Faucher
    Department of Microbiology and Molecular Genetics, The Markey Center for Molecular Genetics, University of Vermont, Stafford Hall, 95 Carrigan Drive, Burlington, VT 05405 0068, USA
    J Mol Biol 387:669-79. 2009
    ..A structural comparison of CacOgg with human OGG1, in complex with 8-oxoG containing DNA, provides a structural rationale for the lack of opposite base specificity displayed by CacOgg...
  43. doi Iron-sulfur cluster coordination in the [FeFe]-hydrogenase H cluster biosynthetic factor HydF
    Neelambari Joshi
    Department of Chemistry and Biochemistry, The Astrobiology Biogeocatalysis Research Center, Montana State University, Bozeman, MT 59717, United States
    FEBS Lett 586:3939-43. 2012
    ..The results implicate a potential role for these residues in biosynthesis specifically and potential in bridging the [4Fe-4S] cluster to 2Fe subcluster biosynthetic intermediates...
  44. ncbi Characterization of two 2[4Fe4S] ferredoxins from Clostridium acetobutylicum
    Olivier Guerrini
    UMR5504, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, CNRS, INRA, INSA, Toulouse F 31400, France
    Curr Microbiol 56:261-7. 2008
    ..The physiological function of CAC3527 is discussed...
  45. pmc The two-component system PhoPR of Clostridium acetobutylicum is involved in phosphate-dependent gene regulation
    Tomas Fiedler
    University of Rostock, Institute of Biological Sciences, Division of Microbiology, Albert Einstein Strasse 3, D 18051 Rostock, Germany
    J Bacteriol 190:6559-67. 2008
    The phoPR gene locus of Clostridium acetobutylicum ATCC 824 comprises two genes, phoP and phoR. Deduced proteins are predicted to represent a response regulator and sensor kinase of a phosphate-dependent two-component regulatory system...
  46. ncbi Sequence and arrangement of genes encoding enzymes of the acetone-production pathway of Clostridium acetobutylicum ATCC824
    D J Petersen
    F A Janssens Laboratorium voor Genetica, K V Leuven, Heverlee, Belgium
    Gene 123:93-7. 1993
    ..The intervening DNA region separating the two transcripts is characterized by an inverted repeat which appears capable of forming a stem-loop structure functioning as a Rho-independent transcription terminator in both directions...
  47. pmc Cloning, sequencing, and molecular analysis of the groESL operon of Clostridium acetobutylicum
    F Narberhaus
    Institut fur Mikrobiologie, Georg August Universitat Gottingen, Germany
    J Bacteriol 174:3282-9. 1992
    ....
  48. pmc mRNA analysis of the adc gene region of Clostridium acetobutylicum during the shift to solventogenesis
    U Gerischer
    Institut fur Mikrobiologie, Universitat Gottingen, Germany
    J Bacteriol 174:426-33. 1992
    ..Gerischer and P. Dürre, J. Bacteriol. 172:6907-6918, 1990), probably is indeed a coding region. ORF2, however, does not seem to have a coding function...
  49. pmc Molecular characterization of the dnaK gene region of Clostridium acetobutylicum, including grpE, dnaJ, and a new heat shock gene
    F Narberhaus
    Institut fur Mikrobiologie, Georg August Universitat Gottingen, Germany
    J Bacteriol 174:3290-9. 1992
    ..Maximum mRNA levels of this heat shock operon were found about 15 min after a heat shock from 30 to 42 degrees C. Our results indicate that orfA codes for an unknown heat shock protein...
  50. pmc Cloning of the Clostridium acetobutylicum ATCC 824 acetyl coenzyme A acetyltransferase (thiolase; EC 2.3.1.9) gene
    D J Petersen
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77251
    Appl Environ Microbiol 57:2735-41. 1991
    ....
  51. ncbi Cloning of an NADH-dependent butanol dehydrogenase gene from Clostridium acetobutylicum
    D J Petersen
    Rice University, Department of Biochemistry and Cell Biology, Houston, Texas 77251
    Ann N Y Acad Sci 646:94-8. 1991
    ..Maxicell analysis of [35S]-radiolabeled plasmid-encoded proteins identified a species encoded by the clostridial insert with the expected Mr of 42 kD...
  52. ncbi Sequence of the lyc gene encoding the autolytic lysozyme of Clostridium acetobutylicum ATCC824: comparison with other lytic enzymes
    C Croux
    Unidad de Genetica Bacteriana, Centro de Investigaciones Biologicas, Madrid, Spain
    Gene 104:25-31. 1991
    ..The existence of five putative repeated motifs in the C-terminal region of the autolytic lysozyme suggests that this region could play a role in the recognition of the polymeric substrate...
  53. pmc Cloning, sequencing, and molecular analysis of the acetoacetate decarboxylase gene region from Clostridium acetobutylicum
    U Gerischer
    Institut fur Mikrobiologie, Universitat Gottingen, Federal Republic of Germany
    J Bacteriol 172:6907-18. 1990
    Acetoacetate decarboxylase (ADC) (EC4.1.1.4) of Clostridium acetobutylicum DSM 792 was purified to homogeneity, and its first 25 N-terminal amino acids were determined...
  54. ncbi The active site of cetoacetate decarboxylase
    R A Laursen
    J Am Chem Soc 88:3426-30. 1966
  55. ncbi Cloning, nucleotide sequence and structural analysis of the Clostridium acetobutylicum dnaJ gene
    S Behrens
    Institut für Mikrobiologie der Georg August Universität, Gottingen, FRG
    FEMS Microbiol Lett 114:53-60. 1993
    ..acetobutylicum. The 5' end (901 bp) of another gene (orfB), downstream of dnaJ and not heat-inducible, showed no significant similarity to other sequences available in EMBL and GenBank databases...
  56. ncbi Solventogenic enzymes of Clostridium acetobutylicum: catalytic properties, genetic organization, and transcriptional regulation
    P Dürre
    Institut fur Mikrobiologie, Georg August Universitat Gottingen, Germany
    FEMS Microbiol Rev 17:251-62. 1995
    ..Although the signal that triggers the shift to solventogenesis has not yet been elucidated, recent investigations suggest a possible function of DNA supercoiling as a transcriptional sensor of the respective environmental stimuli...
  57. ncbi Sequence and arrangement of genes encoding sigma factors in Clostridium acetobutylicum ATCC 824
    J Wong
    Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77251
    Gene 153:89-92. 1995
    The nucleotide sequence of a 2.7-kb region of Clostridium acetobutylicum ATCC 824 DNA containing three open reading frames was determined...
  58. pmc Sporulation and primary sigma factor homologous genes in Clostridium acetobutylicum
    U Sauer
    Institut fur Mikrobiologie, Georg August Universitat Gottingen, Germany
    J Bacteriol 176:6572-82. 1994
    Using a PCR-based approach, we have cloned various sigma factor homologous genes from Clostridium acetobutylicum DSM 792. The nucleotide sequence of the dnaE-sigA operon has been determined and predicts two genes encoding 69- and 43-kDa ..
  59. ncbi Characterization of a maltose transport system in Clostridium acetobutylicum ATCC 824
    M Tangney
    Department of Biological Sciences, Heriot Watt University, Riccarton, Edinburgh EH14 4AS, Scotland, UK
    J Ind Microbiol Biotechnol 27:298-306. 2001
    The utilization of maltose by Clostridium acetobutylicum ATCC 824 was investigated. Glucose was used preferentially to maltose, when both substrates were present in the medium...
  60. pmc Sequence and molecular characterization of a DNA region encoding a small heat shock protein of Clostridium acetobutylicum
    U Sauer
    Institut fur Mikrobiologie, Georg August Universitat Gottingen, Germany
    J Bacteriol 175:3394-400. 1993
    ..A putative rho-independent transcription termination structure was identified at the 3' end of hsp18...
  61. ncbi Mechanism of the reaction catalyzed by acetoacetate decarboxylase. Importance of lysine 116 in determining the pKa of active-site lysine 115
    L A Highbarger
    Department of Chemistry and Biochemistry, University of Maryland, College Park 20742, USA
    Biochemistry 35:41-6. 1996
    ..9. These data support Westheimer's hypothesis that the pKa of the Schiff base-forming Lys 115 is decreased by its spatial proximity to the epsilon-ammonium group of Lys 116...
  62. pmc The kdp system of Clostridium acetobutylicum: cloning, sequencing, and transcriptional regulation in response to potassium concentration
    A Treuner-Lange
    Angewandte Mikrobiologie und Mykologie, Universitat Ulm, Germany
    J Bacteriol 179:4501-12. 1997
    ..Except for the two small peptides, all gene products could be detected in in vitro transcription-translation experiments...
  63. ncbi Complementation of an Escherichia coli polypeptide deformylase mutant with a gene from Clostridium acetobutylicum ATCC 824
    E Belouski
    Rice University, Department of Biochemistry and Cell Biology, 6100 Main Street, Houston, TX 77005 1892, USA
    Curr Microbiol 36:248-9. 1998
    The Clostridium acetobutylicum ATCC 824 DNA containing the 3' end of a PriA homolog, deformylase (def), and the 5' end of formyltransferase (fmt) has been cloned, sequenced, and used to complement an Escherichia coli mutant...
  64. ncbi Sporulation and time course expression of sigma-factor homologous genes in Clostridium acetobutylicum
    J D Santangelo
    Institut fur Mikrobiologie, Georg August Universitat, Gottingen, Germany
    FEMS Microbiol Lett 161:157-64. 1998
    ..From primer extension experiments promoter structures could be deduced with high homology to the Bacillus consensus. Upstream of the spoIIGA-sigE operon a gene with significant similarity to ftsZ could be detected...
  65. ncbi Two-dimensional gel electrophoresis separation and N-terminal sequence analysis of proteins from Clostridium pasteurianum W5
    R Flengsrud
    Department of Biotechnological Sciences, Agricultural University of Norway, As
    Electrophoresis 19:802-6. 1998
    ..Amino-terminal sequence determination and sequence analysis allowed the identification of 20 proteins, while 11 protein sequences remained unidentified and one protein appeared to have a blocked amino terminus...
  66. ncbi The glyceraldehyde-3-phosphate dehydrogenase of Clostridium acetobutylicum: isolation and purification of the enzyme, and sequencing and localization of the gap gene within a cluster of other glycolytic genes
    W Schreiber
    Universitat Ulm, Germany
    Microbiology 145:1839-47. 1999
    ..No sequences resembling rho-independent transcription terminators were found in the intergenic regions. A plasmid carrying the clostridial gap gene complemented an Escherichia coli gap mutant...
  67. ncbi Sequence analysis of the atp operon of Clostridium acetobutylicum DSM 792 encoding the F0F1 ATP synthase
    T Externbrink
    Mikrobiologie und Biotechnologie, Universitat Ulm, Germany
    DNA Seq 11:109-18. 2000
    The atp gene region of Clostridium acetobutylicum DSM 792 has been fully sequenced...
  68. ncbi Differential regulation of two thiolase genes from Clostridium acetobutylicum DSM 792
    K Winzer
    Mikrobiologie und Biotechnologie, Universitat Ulm, Germany
    J Mol Microbiol Biotechnol 2:531-41. 2000
    ..Two thiolase genes (thlA and thIB) have been cloned and sequenced from Clostridium acetobutylicum DSM 792, showing high homology to each other and to thiolases of PHA-synthesizing bacteria...
  69. ncbi Molecular analysis of the mannitol operon of Clostridium acetobutylicum encoding a phosphotransferase system and a putative PTS-modulated regulator
    S Behrens
    Institut fur Mikrobiologie, Georg August Universitat Gottingen, Grisebachstrasse 8, D 37077 Gottingen, Germany
    Microbiology 147:75-86. 2001
    b>Clostridium acetobutylicum DSM 792 accumulates and phosphorylates mannitol via a phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS)...
  70. ncbi Possible function of tRNA(Thr)ACG in regulation of solvent formation in Clostridium acetobutylicum
    U Sauer
    Institut fur Mikrobiologie, Universitat Gottingen, FRG
    FEMS Microbiol Lett 100:147-53. 1992
    ..Evolutionary aspects of this codon selection and a possible translational regulation mechanism are discussed...

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