Tetrahymena thermophila SB210

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Top Publications

  1. Pearson C, Osborn D, Giddings T, Beales P, Winey M. Basal body stability and ciliogenesis requires the conserved component Poc1. J Cell Biol. 2009;187:905-20 pubmed publisher
    ..A second dynamic population assembles throughout the cell cycle. Our experiments identify novel roles for Poc1 in centriole stability and ciliogenesis. ..
  2. Wloga D, Webster D, Rogowski K, Bré M, Levilliers N, Jerka Dziadosz M, et al. TTLL3 Is a tubulin glycine ligase that regulates the assembly of cilia. Dev Cell. 2009;16:867-76 pubmed publisher
    ..We propose that tubulin glycylation regulates the assembly and dynamics of axonemal microtubules and acts either directly or indirectly by inhibiting tubulin glutamylation. ..
  3. Zweifel E, Smith J, Romero D, Giddings T, Winey M, Honts J, et al. Nested genes CDA12 and CDA13 encode proteins associated with membrane trafficking in the ciliate Tetrahymena thermophila. Eukaryot Cell. 2009;8:899-912 pubmed publisher
    ..thermophila. We also highlight an unusual aspect of genome organization in Tetrahymena, namely, the existence of nested, antisense genes...
  4. Eisen J, Coyne R, Wu M, Wu D, Thiagarajan M, Wortman J, et al. Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote. PLoS Biol. 2006;4:e286 pubmed
    ..thermophila an ideal model for functional genomic studies to address biological, biomedical, and biotechnological questions of fundamental importance...
  5. Cervantes M, Xi X, Vermaak D, Yao M, Malik H. The CNA1 histone of the ciliate Tetrahymena thermophila is essential for chromosome segregation in the germline micronucleus. Mol Biol Cell. 2006;17:485-97 pubmed
    ..This study reveals a strong dependence of the germline micronucleus on centromeric histones for proper chromosome segregation...
  6. Glover C, Gorovsky M. Amino-acid sequence of Tetrahymena histone H4 differs from that of higher eukaryotes. Proc Natl Acad Sci U S A. 1979;76:585-9 pubmed
  7. Davie J, Lin R, Allis C. Timing of the appearance of ubiquitinated histones in developing new macronuclei of Tetrahymena thermophila. Biochem Cell Biol. 1991;69:66-71 pubmed
  8. Galati D, Bonney S, Kronenberg Z, Clarissa C, Yandell M, Elde N, et al. DisAp-dependent striated fiber elongation is required to organize ciliary arrays. J Cell Biol. 2014;207:705-15 pubmed publisher
    ..Therefore, DisAp is a novel KF component that is essential for force-dependent KF elongation and BB orientation in multiciliary arrays. ..
  9. Ren Q, Gorovsky M. The nonessential H2A N-terminal tail can function as an essential charge patch on the H2A.Z variant N-terminal tail. Mol Cell Biol. 2003;23:2778-89 pubmed

More Information

Publications59

  1. Bannon G, Bowen J, Yao M, Gorovsky M. Tetrahymena H4 genes: structure, evolution and organization in macro- and micronuclei. Nucleic Acids Res. 1984;12:1961-75 pubmed
  2. Lukaszewicz A, Shodhan A, Loidl J. Exo1 and Mre11 execute meiotic DSB end resection in the protist Tetrahymena. DNA Repair (Amst). 2015;35:137-43 pubmed publisher
    ..Consistent with the absence of the ZMM crossover pathway in Tetrahymena, crossover formation is independent of Exo1. ..
  3. Seixas C, Cruto T, Tavares A, Gaertig J, Soares H. CCTalpha and CCTdelta chaperonin subunits are essential and required for cilia assembly and maintenance in Tetrahymena. PLoS ONE. 2010;5:e10704 pubmed publisher
    ..Our results demonstrate that the CCT subunits are essential and required for ciliary assembly and maintenance of axoneme structure, especially at the tips of cilia. ..
  4. Kilpatrick A, Honts J, Sleister H, Fowler C. Solution NMR structures of the C-domain of Tetrahymena cytoskeletal protein Tcb2 reveal distinct calcium-induced structural rearrangements. Proteins. 2016;84:1748-1756 pubmed publisher
    ..Proteins 2016; 84:1748-1756. © 2016 Wiley Periodicals, Inc. ..
  5. Nomoto M, Imai N, Saiga H, Matsui T, Mita T. Characterization of two types of histone H2B genes from macronuclei of Tetrahymena thermophila. Nucleic Acids Res. 1987;15:5681-97 pubmed
    ..The results clearly indicated that two cloned histone H2B genes were transcribed, giving rise to the major histone H2B mRNAs with a UAA triplet sequence in frame. The tentative 5'- and 3'-ends of histone H2B mRNAs were determined. ..
  6. Tomazic M, Najle S, Nusblat A, Uttaro A, Nudel C. A novel sterol desaturase-like protein promoting dealkylation of phytosterols in Tetrahymena thermophila. Eukaryot Cell. 2011;10:423-34 pubmed publisher
    ..Sterol analysis upon culture with various substrates and inhibitors indicate that the removal of the C-24 ethyl group in Tetrahymena may proceed by a mechanism different from the one currently known. ..
  7. Zhang C, Molascon A, Gao S, Liu Y, Andrews P. Quantitative proteomics reveals that the specific methyltransferases Txr1p and Ezl2p differentially affect the mono-, di- and trimethylation states of histone H3 lysine 27 (H3K27). Mol Cell Proteomics. 2013;12:1678-88 pubmed publisher
    ..Our studies also delineate some of the interdependences between various H3 modifications, as compensatory increases in monomethylation at H3K4, H3K23, and H3K56 were also observed for both TXR1 and ELZ2 mutants. ..
  8. Zhang C, Gao S, Molascon A, Liu Y, Andrews P. Quantitative proteomics reveals histone modifications in crosstalk with H3 lysine 27 methylation. Mol Cell Proteomics. 2014;13:749-59 pubmed publisher
    ..We also provide additional evidence implicating hyper-acetylation in the DNA damage response pathway in replication-defective ?TXR1 cells, in apparent contrast to the transcriptional role of hyper-acetylation in ?EZL2 cells. ..
  9. Vavra K, Allis C, Gorovsky M. Regulation of histone acetylation in Tetrahymena macro- and micronuclei. J Biol Chem. 1982;257:2591-8 pubmed
  10. Garg J, Lambert J, Karsou A, Marquez S, Nabeel Shah S, Bertucci V, et al. Conserved Asf1-importin ? physical interaction in growth and sexual development in the ciliate Tetrahymena thermophila. J Proteomics. 2013;94:311-26 pubmed publisher
    ..These studies set the stage for further use of functional proteomics to elucidate details of the characterization and functional analysis of the unique chromatin domains in T. thermophila...
  11. Akiyama B, Gomez A, Stone M. A conserved motif in Tetrahymena thermophila telomerase reverse transcriptase is proximal to the RNA template and is essential for boundary definition. J Biol Chem. 2013;288:22141-9 pubmed publisher
    ..Taken together, these results suggest a model in which the CP2 motif binds the TBE to strictly define which TER nucleotides can be reverse transcribed...
  12. Gao S, Xiong J, Zhang C, Berquist B, Yang R, Zhao M, et al. Impaired replication elongation in Tetrahymena mutants deficient in histone H3 Lys 27 monomethylation. Genes Dev. 2013;27:1662-79 pubmed publisher
    ..Additionally, we demonstrated interactions between TXR1 and proliferating cell nuclear antigen (PCNA). These findings support a conserved pathway through which H3K27me1 facilitates replication elongation...
  13. Vonderfecht T, Cookson M, Giddings T, Clarissa C, Winey M. The two human centrin homologues have similar but distinct functions at Tetrahymena basal bodies. Mol Biol Cell. 2012;23:4766-77 pubmed publisher
    ..A further examination of human centrin 3 homologues shows that they function in a manner distinct from human centrin 2 homologues. Our data suggest that basal bodies require a centrin from both groups in order to function correctly...
  14. Couvillion M, Bounova G, Purdom E, Speed T, Collins K. A Tetrahymena Piwi bound to mature tRNA 3' fragments activates the exonuclease Xrn2 for RNA processing in the nucleus. Mol Cell. 2012;48:509-20 pubmed publisher
    ..Our findings suggest a role for an Ago/Piwi protein and 3' tRNA fragments in nuclear RNA metabolism...
  15. Singh M, Wang Z, Koo B, Patel A, Cascio D, Collins K, et al. Structural basis for telomerase RNA recognition and RNP assembly by the holoenzyme La family protein p65. Mol Cell. 2012;47:16-26 pubmed publisher
    ..Additionally, our studies define a structurally homologous domain (xRRM) in genuine La and LARP7 proteins and suggest a general mode of RNA binding for biogenesis of their diverse RNA targets...
  16. Liang H, Xu J, Zhao D, Tian H, Yang X, Liang A, et al. Subcellular localization and role of Ran1 in Tetrahymena thermophila amitotic macronucleus. FEBS J. 2012;279:2520-33 pubmed publisher
    ..These results suggest that Ran GTPase pathway is involved in assembly of a specialized intramacronuclear microtubule network and coordinates amitotic progression in Tetrahymena...
  17. Lin I, Chao J, Yao M. An essential role for the DNA breakage-repair protein Ku80 in programmed DNA rearrangements in Tetrahymena thermophila. Mol Biol Cell. 2012;23:2213-25 pubmed publisher
    ..Together the results suggest multiple roles for Tku80p and indicate that a Ku-dependent DNA-repair pathway is involved in programmed DNA rearrangements in Tetrahymena...
  18. Lu X, Xiong J, Yuan D, Miao W. [Alternative splicing of an ATP-binding cassette transporter ABCC10 in Tetrahymena thermophila]. Dongwuxue Yanjiu. 2011;32:605-10 pubmed publisher
    ..Thus, we infer that this mechanism is highly evolutionary conserved in eukaryotes and was already functional in the last eukaryotic common ancestor...
  19. Klinge S, Voigts Hoffmann F, Leibundgut M, Arpagaus S, Ban N. Crystal structure of the eukaryotic 60S ribosomal subunit in complex with initiation factor 6. Science. 2011;334:941-8 pubmed publisher
    ..Furthermore, it elucidates the molecular basis of the interaction with eIF6 and provides a structural framework for further studies of ribosome-associated diseases and the role of the 60S subunit in the initiation of protein synthesis...
  20. Chang Y, Feng L, Xiong J, Miao W. [Function comparison and evolution analysis of metallothionein gene MTT2 and MTT4 in Tetrahymena thermophila]. Dongwuxue Yanjiu. 2011;32:476-84 pubmed publisher
    ..The high similarities in gene structures and functions indicate that MTT2 and MTT4 were generated by recent gene duplication following the dosage balance model...
  21. Wang Q, Xu J, Chai B, Liang A, Wang W. Functional comparison of metallothioneins MTT1 and MTT2 from Tetrahymena thermophila. Arch Biochem Biophys. 2011;509:170-6 pubmed publisher
    ..These results further suggest MTT1 possibly play a role in the detoxification of heavy metal ions, and MTT2 may be involved in the homeostasis of copper ions...
  22. Dave D, Wloga D, Sharma N, Gaertig J. DYF-1 Is required for assembly of the axoneme in Tetrahymena thermophila. Eukaryot Cell. 2009;8:1397-406 pubmed publisher
    ..Our observations support the conserved function for DYF-1 in axoneme assembly or stability but also show that the consequences of loss of DYF-1 for axoneme segments are organism specific...
  23. Min B, Collins K. Multiple mechanisms for elongation processivity within the reconstituted tetrahymena telomerase holoenzyme. J Biol Chem. 2010;285:16434-43 pubmed publisher
    ..Overall, our findings reveal multiple mechanisms and multiple surfaces of protein-DNA and protein-protein interaction that give rise to elongation processivity in the synthesis of a single-stranded nucleic acid product...
  24. Thatcher T, MacGaffey J, Bowen J, Horowitz S, Shapiro D, Gorovsky M. Independent evolutionary origin of histone H3.3-like variants of animals and Tetrahymena. Nucleic Acids Res. 1994;22:180-6 pubmed
    ..Bains, W., and Kedes, L. 1986, J. Mol. Evol., 23: 224-241). These results suggest it is the replication independence and not the particular protein sequence that is important in the function of H3 replacement variants...
  25. Kurth H, Mochizuki K. 2'-O-methylation stabilizes Piwi-associated small RNAs and ensures DNA elimination in Tetrahymena. RNA. 2009;15:675-85 pubmed publisher
    ..This study clearly shows that 3'-terminal 2'-O-methylation on a selected class of small RNAs regulates the function of a specific RNAi pathway...
  26. Liu X, Gorovsky M. Cloning and characterization of the major histone H2A genes completes the cloning and sequencing of known histone genes of Tetrahymena thermophila. Nucleic Acids Res. 1996;24:3023-30 pubmed
    ..With the inclusion of these two genes, T. thermophila is the first organism whose entire complement of known core and linker histones, including replication-dependent and basal variants, has been cloned and sequenced...
  27. Ren Q, Gorovsky M. Histone H2A.Z acetylation modulates an essential charge patch. Mol Cell. 2001;7:1329-35 pubmed
    ..These properties argue that the essential function of H2A.Z acetylation is to modify a "charge patch" by reducing the charge of the tail...
  28. Horowitz S, Bowen J, Bannon G, Gorovsky M. Unusual features of transcribed and translated regions of the histone H4 gene family of Tetrahymena thermophila. Nucleic Acids Res. 1987;15:141-60 pubmed
    ..Downstream of both genes are sequences similar to the processing/polyadenylation signal of higher eukaryotes, although the unique 3' ends are not those predicted by the location of the signals...
  29. Cui B, Liu Y, Gorovsky M. Deposition and function of histone H3 variants in Tetrahymena thermophila. Mol Cell Biol. 2006;26:7719-30 pubmed publisher
    ..Minor H3s are also required to produce viable sexual progeny and play an unexpected role in the germ line micronuclei late in conjugation that is unrelated to transcription...
  30. Nusblat A, Najle S, Tomazic M, Uttaro A, Nudel C. C-5(6) sterol desaturase from tetrahymena thermophila: Gene identification and knockout, sequence analysis, and comparison to other C-5(6) sterol desaturases. Eukaryot Cell. 2009;8:1287-97 pubmed publisher
    ..A phylogenetic analysis placed T. thermophila's enzyme and Paramecium orthologues in a cluster together with functionally characterized C-5 sterol desaturases from vertebrates, fungi, and plants, although in a different branch...
  31. Cui B, Gorovsky M. Centromeric histone H3 is essential for vegetative cell division and for DNA elimination during conjugation in Tetrahymena thermophila. Mol Cell Biol. 2006;26:4499-510 pubmed publisher
  32. Sobel R, Cook R, Perry C, Annunziato A, Allis C. Conservation of deposition-related acetylation sites in newly synthesized histones H3 and H4. Proc Natl Acad Sci U S A. 1995;92:1237-41 pubmed
    ..Thus, although the pattern and sites of deposition-related acetylation appear to be highly conserved in H4, the same does not appear to be the case for histone H3...
  33. van Daal A, White E, Elgin S, Gorovsky M. Conservation of intron position indicates separation of major and variant H2As is an early event in the evolution of eukaryotes. J Mol Evol. 1990;30:449-55 pubmed
    ..In addition, it appears that the variant histone H2A proteins analyzed here are more conserved than the major histone H2A proteins...
  34. Liu X, Gorovsky M. Mapping the 5' and 3' ends of Tetrahymena thermophila mRNAs using RNA ligase mediated amplification of cDNA ends (RLM-RACE). Nucleic Acids Res. 1993;21:4954-60 pubmed
    ..The four histone messages analyzed have contained three sequence elements, TGTGT-TAA-AAGTATT, not found in non-histone messages. Two non-histone messages contained GCATT(N)15ATACC near the poly(A) addition site...
  35. Stargell L, Bowen J, Dadd C, Dedon P, Davis M, Cook R, et al. Temporal and spatial association of histone H2A variant hv1 with transcriptionally competent chromatin during nuclear development in Tetrahymena thermophila. Genes Dev. 1993;7:2641-51 pubmed
  36. Barkauskaite E, Brassington A, Tan E, Warwicker J, Dunstan M, Baños B, et al. Visualization of poly(ADP-ribose) bound to PARG reveals inherent balance between exo- and endo-glycohydrolase activities. Nat Commun. 2013;4:2164 pubmed publisher
    ..Our data reveal the mechanism of poly-ADP-ribosylation reversal, with ADP-ribose as the dominant product, and suggest that the release of apoptotic PAR fragments occurs at unusual PAR/PARG ratios...
  37. Liu X, Li B. Essential and nonessential histone H2A variants in Tetrahymena thermophila. Mol Cell Biol. 1996;16:4305-11 pubmed
    ..thermophila cells, the endogenous chromosomal HTA3 could be completely replaced, defining a gene replacement strategy that can be used to analyze the function of essential genes...
  38. Bayless B, Giddings T, Winey M, Pearson C. Bld10/Cep135 stabilizes basal bodies to resist cilia-generated forces. Mol Biol Cell. 2012;23:4820-32 pubmed publisher
    ..Thus Bld10/Cep135 acts to maintain the structural integrity of basal bodies against the forces of ciliary beating in addition to its separable role in basal body assembly...
  39. Poux A, Marmorstein R. Molecular basis for Gcn5/PCAF histone acetyltransferase selectivity for histone and nonhistone substrates. Biochemistry. 2003;42:14366-74 pubmed publisher
    ..Together, these studies provide a framework for understanding the substrate selectivity of HAT proteins...
  40. Medzihradszky K, Zhang X, Chalkley R, Guan S, McFarland M, Chalmers M, et al. Characterization of Tetrahymena histone H2B variants and posttranslational populations by electron capture dissociation (ECD) Fourier transform ion cyclotron mass spectrometry (FT-ICR MS). Mol Cell Proteomics. 2004;3:872-86 pubmed publisher
    ..This study establishes that integration of the information from these two datasets provides a comprehensive map of posttranslational occupancy for each particular covalent assemblage selected for structural investigation...
  41. Allis C, Richman R, Gorovsky M, Ziegler Y, Touchstone B, Bradley W, et al. hv1 is an evolutionarily conserved H2A variant that is preferentially associated with active genes. J Biol Chem. 1986;261:1941-8 pubmed
    ..Z and H2A.X...
  42. Garcia B, Hake S, Diaz R, Kauer M, Morris S, Recht J, et al. Organismal differences in post-translational modifications in histones H3 and H4. J Biol Chem. 2007;282:7641-55 pubmed publisher
    ..Additionally, modification profiles of H4 acetylation were very similar among the organisms examined...
  43. Song X, Gjoneska E, Ren Q, Taverna S, Allis C, Gorovsky M. Phosphorylation of the SQ H2A.X motif is required for proper meiosis and mitosis in Tetrahymena thermophila. Mol Cell Biol. 2007;27:2648-60 pubmed publisher
    ..These results demonstrate multiple roles of H2A.X phosphorylation in maintaining genomic integrity in different phases of the Tetrahymena life cycle...
  44. D az S, Amaro F, Rico D, Campos V, Ben tez L, Mart n Gonz lez A, et al. Tetrahymena metallothioneins fall into two discrete subfamilies. PLoS ONE. 2007;2:e291 pubmed publisher
  45. Tsao C, Gorovsky M. Different effects of Tetrahymena IFT172 domains on anterograde and retrograde intraflagellar transport. Mol Biol Cell. 2008;19:1450-61 pubmed publisher
    ..These results implicate different regions of Ift172p in different steps of the IFT process...
  46. Bright L, Kambesis N, Nelson S, Jeong B, Turkewitz A. Comprehensive analysis reveals dynamic and evolutionary plasticity of Rab GTPases and membrane traffic in Tetrahymena thermophila. PLoS Genet. 2010;6:e1001155 pubmed publisher
    ..Thus, characterizing entire gene families can provide insight into the evolutionary flexibility of fundamental cellular pathways...
  47. Akella J, Wloga D, Kim J, Starostina N, Lyons Abbott S, Morrissette N, et al. MEC-17 is an alpha-tubulin acetyltransferase. Nature. 2010;467:218-22 pubmed publisher
    ..In summary, we identify MEC-17 as an enzyme that acetylates the K40 residue of alpha-tubulin, the only PTM known to occur on the luminal surface of microtubules...
  48. Yin L, Gater S, Karrer K. A developmentally regulated gene, ASI2, is required for endocycling in the macronuclear anlagen of Tetrahymena. Eukaryot Cell. 2010;9:1343-53 pubmed publisher
    ..Other molecular events in Mac anlage development, including developmentally regulated DNA rearrangement, occur normally in matings between ASI2 knockouts, suggesting that ASI2 specifically regulates endocycling in Tetrahymena...
  49. White E, Shapiro D, Allis C, Gorovsky M. Sequence and properties of the message encoding Tetrahymena hv1, a highly evolutionarily conserved histone H2A variant that is associated with active genes. Nucleic Acids Res. 1988;16:179-98 pubmed
    ..Unusual features in the 3' flanking sequence and in codon usage are also described. Evidence is also presented showing that hv1 message amounts are ten-fold greater in growing cells than in starved cells...
  50. Nickel B, Allis C, Davie J. Ubiquitinated histone H2B is preferentially located in transcriptionally active chromatin. Biochemistry. 1989;28:958-63 pubmed
    ..These observations are consistent with the hypothesis that ubiquitinated histones have a role maintaining the structure of transcriptionally active chromatin...