Thermosynechococcus elongatus BP-1

Summary

Alias: Thermosynechococcus elongatus str. BP-1, Thermosynechococcus elongatus strain BP-1

Top Publications

  1. Iwase R, Imada K, Hayashi F, Uzumaki T, Morishita M, Onai K, et al. Functionally important substructures of circadian clock protein KaiB in a unique tetramer complex. J Biol Chem. 2005;280:43141-9 pubmed
    ..These data suggest that the positively charged cleft and flanking negatively charged ridges in KaiB are essential for the biological function of KaiB in the circadian molecular machinery in cyanobacteria. ..
  2. Okada K, Hase T. Cyanobacterial non-mevalonate pathway: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase interacts with ferredoxin in Thermosynechococcus elongatus BP-1. J Biol Chem. 2005;280:20672-9 pubmed publisher
    ..The result demonstrated that PetF has the ability to transfer electrons to GcpE. Thus, the combined data provide the first evidence that GcpE is a ferredoxin-dependent enzyme in T. elongatus BP-1...
  3. Hayashi F, Itoh N, Uzumaki T, Iwase R, Tsuchiya Y, Yamakawa H, et al. Roles of two ATPase-motif-containing domains in cyanobacterial circadian clock protein KaiC. J Biol Chem. 2004;279:52331-7 pubmed
    ..Additional ATP then binds to the C-terminal low affinity site, stabilizing and phosphorylating the hexamer. We discussed the effect of these KaiC mutations on circadian bioluminescence rhythm in cells of cyanobacteria. ..
  4. Valencia S J, Bitou K, Ishii K, Murakami R, Morishita M, Onai K, et al. Phase-dependent generation and transmission of time information by the KaiABC circadian clock oscillator through SasA-KaiC interaction in cyanobacteria. Genes Cells. 2012;17:398-419 pubmed publisher
    ..Numerical simulations suggest that circadian time information (free phosphorylated SasA) is released mainly by unphosphorylated KaiC during the late subjective night. ..
  5. Murakami R, Miyake A, Iwase R, Hayashi F, Uzumaki T, Ishiura M. ATPase activity and its temperature compensation of the cyanobacterial clock protein KaiC. Genes Cells. 2008;13:387-95 pubmed publisher
  6. Hayashi F, Iwase R, Uzumaki T, Ishiura M. Hexamerization by the N-terminal domain and intersubunit phosphorylation by the C-terminal domain of cyanobacterial circadian clock protein KaiC. Biochem Biophys Res Commun. 2006;348:864-72 pubmed
    ..We propose that the KaiC hexamer consists of a rigid ring structure formed by six N-terminal domains with hexamerization activity and a flexible structure formed by six C-terminal domains with intersubunit phosphorylation activity. ..
  7. Ye S, Vakonakis I, Ioerger T, LiWang A, Sacchettini J. Crystal structure of circadian clock protein KaiA from Synechococcus elongatus. J Biol Chem. 2004;279:20511-8 pubmed
    ..The structure suggests that the KaiB binding site is covered in the dimer interface of the KaiA "closed" conformation, observed in the crystal structure, which suggests an allosteric regulation mechanism. ..
  8. Hayashi F, Suzuki H, Iwase R, Uzumaki T, Miyake A, Shen J, et al. ATP-induced hexameric ring structure of the cyanobacterial circadian clock protein KaiC. Genes Cells. 2003;8:287-96 pubmed
    ..ATP-induced KaiC hexamerization is necessary for the clock function of KaiC. ..
  9. Laughlin T, Bayne A, Trempe J, Savage D, Davies K. Structure of the complex I-like molecule NDH of oxygenic photosynthesis. Nature. 2019;566:411-414 pubmed publisher
    ..These results suggest that NDH could possess several electron transfer routes, which would serve to maximize plastoquinone reduction and avoid deleterious off-target chemistry of the semi-plastoquinone radical. ..

More Information

Publications95

  1. Kuroi K, Okajima K, Ikeuchi M, Tokutomi S, Terazima M. Transient conformational fluctuation of TePixD during a reaction. Proc Natl Acad Sci U S A. 2014;111:14764-9 pubmed publisher
    ..To our knowledge, this is the first report showing enhanced fluctuations of intermediate species during a protein reaction, supporting the importance of fluctuations. ..
  2. Chen H, Liu Q, Zhao J, Jiang P. Biosynthesis, spectral properties and thermostability of cyanobacterial allophycocyanin holo-? subunits. Int J Biol Macromol. 2016;88:88-92 pubmed publisher
    ..0 and stable in pH range of 5.0-7.0, with residual fluorescence intensity no less than 90% of the maximum fluorescence. These findings will pave the way for further protein engineering to achieve high stable APC from extremophiles. ..
  3. Kondo K, Takeyama Y, Sunamura E, Madoka Y, Fukaya Y, Isu A, et al. Amputation of a C-terminal helix of the ? subunit increases ATP-hydrolysis activity of cyanobacterial F1 ATP synthase. Biochim Biophys Acta Bioenerg. 2018;1859:319-325 pubmed publisher
  4. Liauw P, Mashiba T, Kopczak M, Wiegand K, Muraki N, Kubota H, et al. Cloning, expression, crystallization and preliminary X-ray studies of the ferredoxin-NAD(P)+ reductase from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012;68:1048-51 pubmed publisher
    ..Crystals of TeFNR without the additional domain belonged to space group P2(1), with unit-cell parameters a = 55.05, b = 71.66, c = 89.73 Å, α = 90, β = 98.21, γ = 90°. ..
  5. Hatanaka H, Tanimura R, Katoh S, Inagaki F. Solution structure of ferredoxin from the thermophilic cyanobacterium Synechococcus elongatus and its thermostability. J Mol Biol. 1997;268:922-33 pubmed
  6. Endo K, Kobayashi K, Wada H. Sulfoquinovosyldiacylglycerol has an Essential Role in Thermosynechococcus elongatus BP-1 Under Phosphate-Deficient Conditions. Plant Cell Physiol. 2016;57:2461-2471 pubmed
    ..elongatus, which is deficient in PG biosynthesis. These data suggest that SQDG has a specific role in the growth and photosynthesis of T. elongatus, which cannot be complemented by PG, particularly under Pi-deficient conditions. ..
  7. Nausch H, Huckauf J, Broer I. Peculiarities and impacts of expression of bacterial cyanophycin synthetases in plants. Appl Microbiol Biotechnol. 2016;100:1559-65 pubmed publisher
    ..2008, 2009; Neumann et al. 2005). It cannot be ascribed to a depletion of arginine, lysine, or aspartate, the substrates for CP synthesis. ..
  8. Iwase R, Imada K, Hayashi F, Uzumaki T, Namba K, Ishiura M. Crystallization and preliminary crystallographic analysis of the circadian clock protein KaiB from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. Acta Crystallogr D Biol Crystallogr. 2004;60:727-9 pubmed
    ..4, c = 93.7 A, beta = 100.1 degrees. Anomalous difference Patterson maps of the Os- and Hg-derivative crystals had significant peaks in their Harker sections, suggesting that both derivatives are suitable for structure determination. ..
  9. Morimoto K, Yamashita E, Kondou Y, Lee S, Arisaka F, Tsukihara T, et al. The asymmetric IscA homodimer with an exposed [2Fe-2S] cluster suggests the structural basis of the Fe-S cluster biosynthetic scaffold. J Mol Biol. 2006;360:117-32 pubmed
  10. Katoh H, Itoh S, Shen J, Ikeuchi M. Functional analysis of psbV and a novel c-type cytochrome gene psbV2 of the thermophilic cyanobacterium Thermosynechococcus elongatus strain BP-1. Plant Cell Physiol. 2001;42:599-607 pubmed
    ..Thus, it is concluded that psbV2 can partly replace the role of psbV in PSII. The close tandem arrangement of psbV/psbV2/petJ implies that psbV2 was created by gene duplication and intergenic recombination during evolution. ..
  11. Sugiura M, Ozaki Y, Nakamura M, Cox N, Rappaport F, Boussac A. The D1-173 amino acid is a structural determinant of the critical interaction between D1-Tyr161 (TyrZ) and D1-His190 in Photosystem II. Biochim Biophys Acta. 2014;1837:1922-31 pubmed
  12. Menon B, Davison P, Hunter C, Scrutton N, Heyes D. Mutagenesis alters the catalytic mechanism of the light-driven enzyme protochlorophyllide oxidoreductase. J Biol Chem. 2010;285:2113-9 pubmed publisher
  13. Sidler W, Gysi J, Isker E, Zuber H. The complete amino acid sequence of both subunits of allophycocyanin, a light harvesting protein-pigment complex from the cyanobacterium Mastigocladus laminosus. Hoppe Seylers Z Physiol Chem. 1981;362:611-28 pubmed
    ..The homology of the alpha- and beta-chains is 37%. A comparison with C-phycocyanin reveals that the second chromophore of the C-phycocyanin beta-subunit is attached to an inserted peptide of 10 amino acid residues at position 151-160. ..
  14. Schneider D, Altenfeld U, Thomas H, Schrader S, Muhlenhoff U, Rogner M. Sequence of the two operons encoding the four core subunits of the cytochrome b(6)f complex from the thermophilic Cyanobacterium synechococcus elongatus. Biochim Biophys Acta. 2000;1491:364-8 pubmed
    ..In addition, five open reading frames with homology to known orfs from the cyanobacterium Synechocystis PCC 6803 were identified in the immediate vicinity of these two operons. ..
  15. Sugiura M, Azami C, Koyama K, Rutherford A, Rappaport F, Boussac A. Modification of the pheophytin redox potential in Thermosynechococcus elongatus Photosystem II with PsbA3 as D1. Biochim Biophys Acta. 2014;1837:139-48 pubmed publisher
    ..The simulation predicted a major effect of the PheoD1/PheoD1(-) potential on (1)O2 yield (~60% in PsbA1-PSII, ~20% in PsbA3-PSII and ~7% in Gln130-PsbA3-PSII), reflecting differential sensitivities to high light. ..
  16. Kerfeld C, Yoshida S, Tran K, Yeates T, Cascio D, Bottin H, et al. The 1.6 A resolution structure of Fe-superoxide dismutase from the thermophilic cyanobacterium Thermosynechococcus elongatus. J Biol Inorg Chem. 2003;8:707-14 pubmed
    ..elongatus FeSOD EPR spectra at pH 5.1, 7.5 and 10.0 are similar. This indicates that no change in the geometry of the Fe(III) site occurs over a wide range of pH. This is in contrast to the other FeSODs described in the literature. ..
  17. Kerfeld C, Sawaya M, Bottin H, Tran K, Sugiura M, Cascio D, et al. Structural and EPR characterization of the soluble form of cytochrome c-550 and of the psbV2 gene product from the cyanobacterium Thermosynechococcus elongatus. Plant Cell Physiol. 2003;44:697-706 pubmed
    ..The UV-visible, EPR and Raman spectra are reported. From the spectroscopic data and from a theoretical structural model based on the cytochrome c-550 structure it is proposed that the 6th ligand of the heme-iron is the Tyr86. ..
  18. Zhang X, Wu H, Huang B, Li Z, Ye Q. One-pot synthesis of glutathione by a two-enzyme cascade using a thermophilic ATP regeneration system. J Biotechnol. 2017;241:163-169 pubmed publisher
    ..The yield based on added l-cysteine reached 81.4% and the productivity of glutathione achieved 5.7mM/h. The one-pot system indicated a potential biotransformation platform for industrial production of glutathione. ..
  19. Berg H, Ziegler K, Piotukh K, Baier K, Lockau W, Volkmer Engert R. Biosynthesis of the cyanobacterial reserve polymer multi-L-arginyl-poly-L-aspartic acid (cyanophycin): mechanism of the cyanophycin synthetase reaction studied with synthetic primers. Eur J Biochem. 2000;267:5561-70 pubmed
    ..Glutamic acid cannot replace aspartic acid as the acidic amino acid, whereas lysine can replace arginine but is incorporated into cyanophycin at a much lower rate...
  20. Kern J, Loll B, Zouni A, Saenger W, Irrgang K, Biesiadka J. Cyanobacterial photosystem II at 3.2 A resolution - the plastoquinone binding pockets. Photosynth Res. 2005;84:153-9 pubmed publisher
    ..Both binding pockets and problems related to the QB site are discussed here and compared to the situation in the purple bacterial reaction centre...
  21. Kamiya N, Shen J. Crystal structure of oxygen-evolving photosystem II from Thermosynechococcus vulcanus at 3.7-A resolution. Proc Natl Acad Sci U S A. 2003;100:98-103 pubmed publisher
    ..In particular, the C terminus of D1 polypeptide was shown to be connected to the Mn cluster directly. The structural information obtained here provides important insights into the mechanism of PSII reactions...
  22. Barthel S, Rupprecht E, Schneider D. Thermostability of two cyanobacterial GrpE thermosensors. Plant Cell Physiol. 2011;52:1776-85 pubmed publisher
    ..Thus, in two related cyanobacteria the GrpE thermosensing function might be mediated by different protein domains...
  23. Loll B, Kern J, Saenger W, Zouni A, Biesiadka J. Towards complete cofactor arrangement in the 3.0 A resolution structure of photosystem II. Nature. 2005;438:1040-4 pubmed publisher
    ..The structure provides information about the Mn4Ca cluster, where oxidation of water takes place. Our study uncovers near-atomic details necessary to understand the processes that convert light to chemical energy...
  24. Iwai M, Katoh H, Katayama M, Ikeuchi M. PSII-Tc protein plays an important role in dimerization of photosystem II. Plant Cell Physiol. 2004;45:1809-16 pubmed publisher
    ..These results appear to be in good agreement with the recent structural model of the dimeric PSII complex...
  25. Baumann B, Sticht H, Sch rpf M, Sutter M, Haehnel W, R sch P. Structure of Synechococcus elongatus [Fe2S2] ferredoxin in solution. Biochemistry. 1996;35:12831-41 pubmed publisher
    ..The overall structure is similar to the structure of the ferredoxin from Anabaena. In contrast to related ferredoxins from mesophilic organisms, this thermostable protein contains a salt bridge inside a 17-amino acid hydrophobic core...
  26. Vakonakis I, LiWang A. Structure of the C-terminal domain of the clock protein KaiA in complex with a KaiC-derived peptide: implications for KaiC regulation. Proc Natl Acad Sci U S A. 2004;101:10925-30 pubmed publisher
    ..It is proposed that modulation of the C-terminal KaiA domain dimerization angle regulates KaiA-KaiC interactions...
  27. Michel K, Exss Sonne P, Scholten Beck G, Kahmann U, Ruppel H, Pistorius E. Immunocytochemical localization of IdiA, a protein expressed under iron or manganese limitation in the mesophilic cyanobacterium Synechococcus PCC 6301 and the thermophilic cyanobacterium Synechococcus elongatus. Planta. 1998;205:73-81 pubmed publisher
    ..A possible explanation for the observation that IdiA was not only expressed under Mn deficiency but also under Fe deficiency is given in the discussion...
  28. Reuter W, Wiegand G, Huber R, Than M. Structural analysis at 2.2 A of orthorhombic crystals presents the asymmetry of the allophycocyanin-linker complex, AP.LC7.8, from phycobilisomes of Mastigocladus laminosus. Proc Natl Acad Sci U S A. 1999;96:1363-8 pubmed
    ..These results will enable interpretations of energy-transfer mechanisms within phycobiliproteins...
  29. Adir N, Lerner N. The crystal structure of a novel unmethylated form of C-phycocyanin, a possible connector between cores and rods in pycobilisomes. J Biol Chem. 2003;278:25926-32 pubmed publisher
  30. Jordan P, Fromme P, Witt H, Klukas O, Saenger W, Krauss N. Three-dimensional structure of cyanobacterial photosystem I at 2.5 A resolution. Nature. 2001;411:909-17 pubmed publisher
    ..The structural information on the proteins and cofactors and their interactions provides a basis for understanding how the high efficiency of photosystem I in light capturing and electron transfer is achieved...
  31. Sato S, Ikeuchi M, Nakamoto H. Expression and function of a groEL paralog in the thermophilic cyanobacterium Thermosynechococcus elongatus under heat and cold stress. FEBS Lett. 2008;582:3389-95 pubmed publisher
    ..The groEL2 gene was dispensable under normal growth conditions at 50 degrees C as a groEL2 disruptant was viable. This groEL2 mutant was highly sensitive to both high and low temperatures...
  32. Krishnamoorthy E, Hassan S, Hanna L, Padmalayam I, Rajaram R, Viswanathan V. Homology modeling of Homo sapiens lipoic acid synthase: Substrate docking and insights on its binding mode. J Theor Biol. 2017;420:259-266 pubmed publisher
    ..This study will facilitate a better understanding mode of action of the enzyme-substrate complex for future studies in designing drugs that can target LIAS protein. ..
  33. Okajima K, Fukushima Y, Suzuki H, Kita A, Ochiai Y, Katayama M, et al. Fate determination of the flavin photoreceptions in the cyanobacterial blue light receptor TePixD (Tll0078). J Mol Biol. 2006;363:10-8 pubmed publisher
    ..Based on the structural and functional similarities of the BLUF and the LOV domain of phototropins, we propose that the interaction between apoprotein and N(5) of flavin determines the photoreaction of the flavin-binding sensors...
  34. Rockwell N, Njuguna S, Roberts L, Castillo E, Parson V, Dwojak S, et al. A second conserved GAF domain cysteine is required for the blue/green photoreversibility of cyanobacteriochrome Tlr0924 from Thermosynechococcus elongatus. Biochemistry. 2008;47:7304-16 pubmed publisher
  35. Nowaczyk M, Hebeler R, Schlodder E, Meyer H, Warscheid B, R gner M. Psb27, a cyanobacterial lipoprotein, is involved in the repair cycle of photosystem II. Plant Cell. 2006;18:3121-31 pubmed publisher
  36. Takahashi R, Okajima K, Suzuki H, Nakamura H, Ikeuchi M, Noguchi T. FTIR study on the hydrogen bond structure of a key tyrosine residue in the flavin-binding blue light sensor TePixD from Thermosynechococcus elongatus. Biochemistry. 2007;46:6459-67 pubmed publisher
    ..The change in the H-bond structure of Tyr8 is coupled to the flavin photoreaction probably through the Tyr8-Gln50-flavin H-bond network, suggesting a significant role of Tyr8 in the photoreaction mechanism of TePixD...
  37. Menon B, Waltho J, Scrutton N, Heyes D. Cryogenic and laser photoexcitation studies identify multiple roles for active site residues in the light-driven enzyme protochlorophyllide oxidoreductase. J Biol Chem. 2009;284:18160-6 pubmed publisher
  38. Lin H, Wang R, Qian Q, Yan M, Meng X, Fu Z, et al. DWARF27, an iron-containing protein required for the biosynthesis of strigolactones, regulates rice tiller bud outgrowth. Plant Cell. 2009;21:1512-25 pubmed publisher
    ..Our results demonstrate that D27 is involved in the MAX/RMS/D pathway, in which D27 acts as a new member participating in the biosynthesis of strigolactones...
  39. Samborska B, Kimber M. A dodecameric CcmK2 structure suggests ?-carboxysomal shell facets have a double-layered organization. Structure. 2012;20:1353-62 pubmed publisher
    ..This model also accurately predicts the observed shell thickness, implying that the ?-carboxysome shell is most likely organized as a double layer. ..
  40. Sugiura M, Rappaport F, Hillier W, Dorlet P, Ohno Y, Hayashi H, et al. Evidence that D1-His332 in photosystem II from Thermosynechococcus elongatus interacts with the S3-state and not with the S2-state. Biochemistry. 2009;48:7856-66 pubmed publisher
    ..All these results suggest that the D1-His332 would be more involved in S(3) than in S(2). This could be one element of the conformational changes put forward in the S(2) to S(3) transition. ..
  41. Schulze J, Schubert W, Moser J, Jahn D, Heinz D. Evolutionary relationship between initial enzymes of tetrapyrrole biosynthesis. J Mol Biol. 2006;358:1212-20 pubmed publisher
    ..We thus propose that the CoA-subfamily (including ALAS) and the aminotransferase subfamily II (including GSAM) are evolutionarily closely related and that ALAS may thus have evolved from GSAM...
  42. Gubernator B, Bartoszewski R, Kroliczewski J, Wildner G, Szczepaniak A. Ribulose-1,5-bisphosphate carboxylase/oxygenase from thermophilic cyanobacterium Thermosynechococcus elongatus. Photosynth Res. 2008;95:101-9 pubmed
    ..This is the first characterization of a "green-like type" rubisco from thermophilic organism. ..
  43. Shimizu T, Hiyama T, Ikeuchi M, Inoue Y. Nucleotide sequence of a metallothionein gene of the thermophilic cyanobacterium Synechococcus vulcanus. Plant Mol Biol. 1992;20:565-7 pubmed
  44. Koike H, Ikeuchi M, Hiyama T, Inoue Y. Identification of photosystem I components from the cyanobacterium, Synechococcus vulcanus by N-terminal sequencing. FEBS Lett. 1989;253:257-63 pubmed
    ..5, 5 and 4.1 kDa components do not correspond to any known proteins except that the sequence of the 4.1 kDa component matches an unidentified open reading frame (ORF) 42 (liverwort) or ORF44 (tobacco) of chloroplast DNA. ..
  45. Motomura T, Suga M, Hienerwadel R, Nakagawa A, Lai T, Nitschke W, et al. Crystal structure and redox properties of a novel cyanobacterial heme protein with a His/Cys heme axial ligation and a Per-Arnt-Sim (PAS)-like domain. J Biol Chem. 2017;292:9599-9612 pubmed publisher
  46. Sone N, Tano H, Ishizuka M. The genes in the thermophilic cyanobacterium Synechococcus vulcanus encoding cytochrome-c oxidase. Biochim Biophys Acta. 1993;1183:130-8 pubmed
    ..Comparison of protein sequences showed that S. vulcanus cytochrome oxidase is closer to Bacillus cytochrome oxidases than the mitochondrial and Paracoccus enzymes, or quinol oxidases from B. subtilis and Escherichia coli. ..
  47. Klukas O, Schubert W, Jordan P, Krauss N, Fromme P, Witt H, et al. Photosystem I, an improved model of the stromal subunits PsaC, PsaD, and PsaE. J Biol Chem. 1999;274:7351-60 pubmed
    ..The 11-transmembrane alpha-helices of these subunits can now be assigned uniquely to the hydrophobic segments identified by hydrophobicity analyses...
  48. Klukas O, Schubert W, Jordan P, Krau N, Fromme P, Witt H, et al. Localization of two phylloquinones, QK and QK', in an improved electron density map of photosystem I at 4-A resolution. J Biol Chem. 1999;274:7361-7 pubmed
  49. Kiseleva L, Serebriiskaya T, Horvath I, Vigh L, Lyukevich A, Los D. Expression of the gene for the delta9 acyl-lipid desaturase in the thermophilic cyanobacterium. J Mol Microbiol Biotechnol. 2000;2:331-8 pubmed
    ..These results suggest that in S. vulcanus the conversion of stearic acid into oleic acid may be controlled not only by the de novo synthesis of the delta9 desaturase but, possibly, by the activation of the pre-existing enzyme. ..
  50. Zouni A, Witt H, Kern J, Fromme P, Krauss N, Saenger W, et al. Crystal structure of photosystem II from Synechococcus elongatus at 3.8 A resolution. Nature. 2001;409:739-43 pubmed publisher
    ..The larger subunits are assigned and the locations and orientations of the cofactors are defined. We also provide new information on the position, size and shape of the manganese cluster, which catalyzes water oxidation...
  51. Nield J, Rizkallah P, Barber J, Chayen N. The 1.45 A three-dimensional structure of C-phycocyanin from the thermophilic cyanobacterium Synechococcus elongatus. J Struct Biol. 2003;141:149-55 pubmed
    ..This is done without touching the crystallization drops throughout the process...
  52. Pattanayek R, Xu Y, Lamichhane A, Johnson C, Egli M. An arginine tetrad as mediator of input-dependent and input-independent ATPases in the clock protein KaiC. Acta Crystallogr D Biol Crystallogr. 2014;70:1375-90 pubmed publisher
    ..Common packing features in KaiC crystals shed light on the KaiB-KaiC interaction. ..
  53. Uzumaki T, Fujita M, Nakatsu T, Hayashi F, Shibata H, Itoh N, et al. Crystal structure of the C-terminal clock-oscillator domain of the cyanobacterial KaiA protein. Nat Struct Mol Biol. 2004;11:623-31 pubmed publisher
    ..KaiA binding to KaiC probably occurs via the concave surface. On the basis of the structure, we predict the structural roles of the residues that affect circadian oscillations...
  54. Davison P, Schubert H, Reid J, Iorg C, Heroux A, Hill C, et al. Structural and biochemical characterization of Gun4 suggests a mechanism for its role in chlorophyll biosynthesis. Biochemistry. 2005;44:7603-12 pubmed publisher
    ..This mechanism could allow Gun4 to mediate magnesium protoporphyrin levels both for chlorophyll biosynthesis and for signaling to the nucleus...
  55. McFarlane M, Hunter C, Heyes D. Kinetic characterisation of the light-driven protochlorophyllide oxidoreductase (POR) from Thermosynechococcus elongatus. Photochem Photobiol Sci. 2005;4:1055-9 pubmed
    ..These results represent the first steady state kinetic characterisation of a thermophilic version of POR. ..
  56. Enomoto G, Nomura R, Shimada T, Narikawa R, Ikeuchi M. Cyanobacteriochrome SesA is a diguanylate cyclase that induces cell aggregation in Thermosynechococcus. J Biol Chem. 2014;289:24801-9 pubmed publisher
    ..Given our results, we propose the name "sesA (sessility-A)" for tlr0924. This is the first report for cyanobacteriochrome-dependent regulation of a sessile/planktonic lifestyle in cyanobacteria via c-di-GMP...
  57. Alaleona F, Franceschini S, Ceci P, Ilari A, Chiancone E. Thermosynechococcus elongatus DpsA binds Zn(II) at a unique three histidine-containing ferroxidase center and utilizes O2 as iron oxidant with very high efficiency, unlike the typical Dps proteins. FEBS J. 2010;277:903-17 pubmed publisher
  58. Mutoh R, Nishimura A, Yasui S, Onai K, Ishiura M. The ATP-mediated regulation of KaiB-KaiC interaction in the cyanobacterial circadian clock. PLoS ONE. 2013;8:e80200 pubmed publisher
    ..KaiC(6mer) that had been hexamerized with ADP plus aluminum fluoride, which are considered to mimic ADP-Pi state, formed a complex with KaiB, suggesting that KaiB is able to associate with KaiC(6mer) with bound ADP-Pi. ..
  59. Adams N, Marklew C, Brindley A, Hunter C, Reid J. Characterization of the magnesium chelatase from Thermosynechococcus elongatus. Biochem J. 2014;457:163-70 pubmed publisher
    ..This loss of co-operativity reveals the significant regulatory role of Synechocystis ChlD...
  60. Okada K. PetH is rate-controlling in the interaction between PetH, a component of the supramolecular complex with photosystem II, and PetF, a light-dependent electron transfer protein. Biochem Biophys Res Commun. 2009;389:394-8 pubmed publisher
    ..The dissociation constant values were determined as approximately 93.65 microM (FNR) for Fd1 and 1.469 mM (Fd1) for FNR. ..
  61. Loll B, Kern J, Zouni A, Saenger W, Biesiadka J, Irrgang K. The antenna system of photosystem II from Thermosynechococcus elongatus at 3.2 A resolution. Photosynth Res. 2005;86:175-84 pubmed
    ..The chlorophyll a in the lumenal layer are less well conserved between Photosystems I and II and even between CP43 and CP47 with 4 chlorophyll a in the former and 7 in the latter. ..
  62. Masoumi A, Heinemann I, Rohde M, Koch M, Jahn M, Jahn D. Complex formation between protoporphyrinogen IX oxidase and ferrochelatase during haem biosynthesis in Thermosynechococcus elongatus. Microbiology. 2008;154:3707-14 pubmed publisher
    ..elongatus cells. Finally, oxygen-dependent coproporphyrinogen III oxidase, which catalyses the formation of protoporphyrinogen IX, was not found to be part of this complex when analysed with the same methodology...
  63. Tano H, Ishizuka M, Sone N. The cytochrome C oxidase genes in blue-green algae and characteristics of the deduced protein sequence for subunit II of the thermophilic cyanobacterium Synechococcus vulcanus. Biochem Biophys Res Commun. 1991;181:437-42 pubmed
    ..The S. vulcanus subunit II does not contain the cytochrome c moiety that is present in bacilli and thermophiles. ..
  64. Shimizu T, Hiyama T, Ikeuchi M, Koike H, Inoue Y. Nucleotide sequence of the psaC gene of the cyanobacterium Synechococcus vulcanus. Nucleic Acids Res. 1990;18:3644 pubmed
  65. Brejc K, Ficner R, Huber R, Steinbacher S. Isolation, crystallization, crystal structure analysis and refinement of allophycocyanin from the cyanobacterium Spirulina platensis at 2.3 A resolution. J Mol Biol. 1995;249:424-40 pubmed publisher
    ..The stereochemistry of the alpha 84 and beta 84 chiral atoms are C(2)-R, C(3)-R and C(31)-R. The configuration (C(4)-Z, C(10)-Z and C(15)-Z) and the conformation (C(5)-anti, C(9)-syn and C(14)-anti) are equal for both chromophores...
  66. Schubert W, Klukas O, Krauss N, Saenger W, Fromme P, Witt H. Photosystem I of Synechococcus elongatus at 4 A resolution: comprehensive structure analysis. J Mol Biol. 1997;272:741-69 pubmed publisher
    ..The assignment of the amino acid sequence to the transmembrane alpha-helices is proposed and likely residues involved in co-ordinating the Chla of the electron transfer system discussed...
  67. Tanaka N, Hiyama T, Nakamoto H. Cloning, characterization and functional analysis of groESL operon from thermophilic cyanobacterium Synechococcus vulcanus. Biochim Biophys Acta. 1997;1343:335-48 pubmed
    ..Introduction of the cloned groEL1 gene into a groEL defective mutant of E. coli resulted in the complementation of heat sensitivity, which contrasted with the previous result with groEL2...
  68. Katoh H, Ikeuchi M. Targeted disruption of psbX and biochemical characterization of photosystem II complex in the thermophilic cyanobacterium Synechococcus elongatus. Plant Cell Physiol. 2001;42:179-88 pubmed
    ..Gel filtration chromatography of the PSII complex revealed that the dimeric structure of the complex was not greatly affected in the psbX-disrupted mutant. ..
  69. Adir N, Dobrovetsky Y, Lerner N. Structure of c-phycocyanin from the thermophilic cyanobacterium Synechococcus vulcanus at 2.5 A: structural implications for thermal stability in phycobilisome assembly. J Mol Biol. 2001;313:71-81 pubmed publisher
    ..vulcanus phycocyanin, enables the formation of important polar interactions at both the (alphabeta) monomer and (alphabeta)(6) hexamer association interfaces...
  70. Fromme P, Jordan P, Krauss N. Structure of photosystem I. Biochim Biophys Acta. 2001;1507:5-31 pubmed
    ..In this review, biochemical data and results of biophysical investigations are discussed with respect to the X-ray crystallographic structure in order to give an overview of the structure and function of this large membrane protein...
  71. Adir N, Vainer R, Lerner N. Refined structure of c-phycocyanin from the cyanobacterium Synechococcus vulcanus at 1.6 A: insights into the role of solvent molecules in thermal stability and co-factor structure. Biochim Biophys Acta. 2002;1556:168-74 pubmed
    ..On the basis of the crystal packing reported here and in comparison to other phycobiliprotein crystal forms, we have analyzed the roles of specific sites on the formation of the phycobilisome complex...
  72. Vakonakis I, Sun J, Wu T, Holzenburg A, Golden S, LiWang A. NMR structure of the KaiC-interacting C-terminal domain of KaiA, a circadian clock protein: implications for KaiA-KaiC interaction. Proc Natl Acad Sci U S A. 2004;101:1479-84 pubmed publisher
    ..This NMR structure and a 21-A-resolution electron microscopy structure of the hexameric KaiC particle allow us to postulate a mode of KaiA-KaiC interaction, in which KaiA binds a linker region connecting two globular KaiC domains...
  73. Ferreira K, Iverson T, Maghlaoui K, Barber J, Iwata S. Architecture of the photosynthetic oxygen-evolving center. Science. 2004;303:1831-8 pubmed publisher
    ..The details of the surrounding coordination sphere of the metal cluster and the implications for a possible oxygen-evolving mechanism are discussed...
  74. Loll B, Gerold G, Slowik D, Voelter W, Jung C, Saenger W, et al. Thermostability and Ca2+ binding properties of wild type and heterologously expressed PsbO protein from cyanobacterial photosystem II. Biochemistry. 2005;44:4691-8 pubmed
    ..Our results are discussed in the light of the recent 3D-structural analysis of the oxygen-evolving PSII and structural/thermodynamic differences between the two homologous proteins from thermophilic cyanobacteria and plants. ..
  75. Kita A, Okajima K, Morimoto Y, Ikeuchi M, Miki K. Structure of a cyanobacterial BLUF protein, Tll0078, containing a novel FAD-binding blue light sensor domain. J Mol Biol. 2005;349:1-9 pubmed publisher
  76. Iwai M, Katayama M, Ikeuchi M. Absence of the psbH gene product destabilizes the Photosystem II complex and prevents association of the Photosystem II-X protein in the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. Photosynth Res. 2006;87:313-22 pubmed
    ..These results suggest that the PS II-H protein is essential for stable assembly of native dimeric PS II complex containing PS II-X. ..
  77. Franceschini S, Ceci P, Alaleona F, Chiancone E, Ilari A. Antioxidant Dps protein from the thermophilic cyanobacterium Thermosynechococcus elongatus. FEBS J. 2006;273:4913-28 pubmed
    ..This catalytic property is of special importance in T. elongatus (which lacks the catalase gene) in the protection of DNA and photosystems I and II from hydrogen peroxide-mediated oxidative damage. ..
  78. Tanaka K, Nakasone Y, Okajima K, Ikeuchi M, Tokutomi S, Terazima M. Oligomeric-state-dependent conformational change of the BLUF protein TePixD (Tll0078). J Mol Biol. 2009;386:1290-300 pubmed
    ..These results demonstrated that only the decamer state is responsible for the conformational change. The results may suggest that the oligomeric state is functionally important in the signal transduction of this photosensory protein. ..
  79. Nakamura A, Akai M, Yoshida E, Taki T, Watanabe T. Reversed-phase HPLC determination of chlorophyll a' and phylloquinone in Photosystem I of oxygenic photosynthetic organisms. Universal existence of one chlorophyll a' molecule in Photosystem I. Eur J Biochem. 2003;270:2446-58 pubmed
    ..These findings confirm that a single Chl a' molecule in P700 is the universal feature of PS I of the Chl a-based oxygenic photosynthetic organisms. ..
  80. Veit S, Takeda K, Tsunoyama Y, Rexroth D, Rögner M, Miki K. Structure of a thermophilic cyanobacterial b6f-type Rieske protein. Acta Crystallogr D Biol Crystallogr. 2012;68:1400-8 pubmed
  81. Iida T, Mutoh R, Onai K, Morishita M, Furukawa Y, Namba K, et al. Importance of the monomer-dimer-tetramer interconversion of the clock protein KaiB in the generation of circadian oscillations in cyanobacteria. Genes Cells. 2015;20:173-90 pubmed publisher
    ..Our findings suggest that KaiB is in equilibrium between a monomer, dimer and tetramer in cyanobacterial cells. ..
  82. Schulze J, Masoumi A, Nickel D, Jahn M, Jahn D, Schubert W, et al. Crystal structure of a non-discriminating glutamyl-tRNA synthetase. J Mol Biol. 2006;361:888-97 pubmed publisher
    ..Most other ND-GluRS share this structural feature, leading to relaxed substrate specificity...
  83. Beissinger M, Sticht H, Sutter M, Ejchart A, Haehnel W, Rosch P. Solution structure of cytochrome c6 from the thermophilic cyanobacterium Synechococcus elongatus. EMBO J. 1998;17:27-36 pubmed
    ..As the structure of PSI is known in S.elongatus, the reported cytochrome c6 structure can provide a basis for mutagenesis studies to delineate the mechanism of electron transfer between both. ..
  84. Krauss N, Schubert W, Klukas O, Fromme P, Witt H, Saenger W. Photosystem I at 4 A resolution represents the first structural model of a joint photosynthetic reaction centre and core antenna system. Nat Struct Biol. 1996;3:965-73 pubmed
    ..This suggests a dual role for these Chl a both in excitation energy and electron transfer. The architecture of the protein core indicates quinone and iron-sulphur type reaction centres to have a common ancestor. ..
  85. Kashino Y, Takahashi T, Inoue Kashino N, Ban A, Ikeda Y, Satoh K, et al. Ycf12 is a core subunit in the photosystem II complex. Biochim Biophys Acta. 2007;1767:1269-75 pubmed
    ..Our finding shows, for the first time, that ycf12 is actually expressed as a component of the PS II complex in the cell, revealing that a previously unidentified transmembrane protein exists in the PS II core complex. ..
  86. Iwai M, Suzuki T, Dohmae N, Inoue Y, Ikeuchi M. Absence of the PsbZ subunit prevents association of PsbK and Ycf12 with the PSII complex in the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. Plant Cell Physiol. 2007;48:1758-63 pubmed
    ..We suggest that Ycf12 is an unidentified membrane-spanning polypeptide that is placed near PsbZ and PsbK in the crystal structure of PSII. ..