Chlorobium tepidum TLS


Alias: Chlorobium tepidum str. TLS, Chlorobaculum tepidum TLS, Chlorobium tepidum strain TLS

Top Publications

  1. Wreggelsworth K, Barker D. A comparison of the protein-coding genomes of two green sulphur bacteria, Chlorobium tepidum TLS and Pelodictyon phaeoclathratiforme BU-1. BMC Res Notes. 2015;8:565 pubmed publisher
    ..were predicted computationally based on sequence similarity across the genome-wide protein sets of Chlorobium tepidum TLS and Pelodictyon phaeoclathratiforme BU-1...
  2. Vassilieva E, Antonkine M, Zybailov B, Yang F, Jakobs C, Golbeck J, et al. Electron transfer may occur in the chlorosome envelope: the CsmI and CsmJ proteins of chlorosomes are 2Fe-2S ferredoxins. Biochemistry. 2001;40:464-73 pubmed
    ..The former potential is similar to that measured by redox titration of the clusters in inclusion bodies of CsmJ. Possible roles for these iron-sulfur proteins in electron transport and light harvesting are discussed...
  3. Kim J, Shin D, Pufan R, Huang C, Yokota H, Kim R, et al. Crystal structure of ScpB from Chlorobium tepidum, a protein involved in chromosome partitioning. Proteins. 2006;62:322-8 pubmed
  4. Hase T, Wakabayashi S, Matsubara H, Evans M, Jennings J. Amino acid sequence of a ferredoxin from Chlorobium thiosulfatophilum strain Tassajara, a photosynthetic green sulfur bacterium. J Biochem. 1978;83:1321-5 pubmed
    ..We compared all the photosynthetic bacterial ferredoxins that have been sequenced and concluded that C. thiosulfatophilum ferredoxin is most closely related to C. limicola ferredoxin I...
  5. Gomez Maqueo Chew A, Frigaard N, Bryant D. Identification of the bchP gene, encoding geranylgeranyl reductase in Chlorobaculum tepidum. J Bacteriol. 2008;190:747-9 pubmed publisher
    ..Each gene was insertionally inactivated, and the resulting mutants were characterized. CT2256 encodes geranylgeranyl reductase (BchP); CT1232 is not involved in bacteriochlorophyll or chlorophyll biosynthesis...
  6. Bagchi A, Ghosh T. Structural interaction between DsrE-DsrF-DsrH proteins involved in the transport of electrons in the dsr operon. J Biomol Struct Dyn. 2008;25:517-23 pubmed
    ..The hexamerization of the proteins would help to bring the Cys residues in close proximity, which enables the complex to actively take part electron transport process. ..
  7. Vassilieva E, Stirewalt V, Jakobs C, Frigaard N, Inoue Sakamoto K, Baker M, et al. Subcellular localization of chlorosome proteins in Chlorobium tepidum and characterization of three new chlorosome proteins: CsmF, CsmH, and CsmX. Biochemistry. 2002;41:4358-70 pubmed
  8. Muraki N, Seo D, Shiba T, Sakurai T, Kurisu G. Asymmetric dimeric structure of ferredoxin-NAD(P)+ oxidoreductase from the green sulfur bacterium Chlorobaculum tepidum: implications for binding ferredoxin and NADP+. J Mol Biol. 2010;401:403-14 pubmed publisher
    ..tepidum FNR. ..
  9. Li Y, Zhou W, Blankenship R, Allen J. Crystal structure of the bacteriochlorophyll a protein from Chlorobium tepidum. J Mol Biol. 1997;271:456-71 pubmed publisher

More Information


  1. Oh oka H, Iwaki M, Itoh S. Viscosity dependence of the electron transfer rate from bound cytochrome c to P840 in the photosynthetic reaction center of the green sulfur bacterium Chlorobium tepidum. Biochemistry. 1997;36:9267-72 pubmed
  2. Li H, Bryant D. Envelope proteins of the CsmB/CsmF and CsmC/CsmD motif families influence the size, shape, and composition of chlorosomes in Chlorobaculum tepidum. J Bacteriol. 2009;191:7109-20 pubmed publisher
  3. Supangat S, Choi Y, Park Y, Son D, Han C, Lee K. Expression, purification, crystallization and preliminary X-ray analysis of sepiapterin reductase from Chlorobium tepidum. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005;61:202-4 pubmed
    ..The structure of CT-SR has been determined using MAD phasing. There is one CT-SR tetramer in the asymmetric unit formed by two closely interacting CT-SR dimers. The solvent content is calculated to be about 67.2%. ..
  4. Maresca J, Romberger S, Bryant D. Isorenieratene biosynthesis in green sulfur bacteria requires the cooperative actions of two carotenoid cyclases. J Bacteriol. 2008;190:6384-91 pubmed publisher
    ..Consequently, the branch point for the synthesis of mono- and dicyclic carotenoids in GSB seems to be the modification of gamma-carotene, rather than the cyclization of lycopene as occurs in cyanobacteria...
  5. Bagchi A. Structural modeling of SoxF protein from Chlorobium tepidum: an approach to understand the molecular basis of thiosulfate oxidation. Biochem Biophys Res Commun. 2011;414:409-11 pubmed publisher
    ..Since this is the first report regarding the structural biology of SoxF protein this study may shed light in the hitherto unknown molecular biochemistry of sulfur anion oxidation by sox operon. ..
  6. Brito C, Matias C, Gonzalez Nilo F, Watt R, Yevenes A. The C-terminal regions have an important role in the activity of the ferroxidase center and the stability of Chlorobium tepidum ferritin. Protein J. 2014;33:211-20 pubmed publisher
    ..Therefore, these results indicate that the C-terminal regions have an important role in the activity of the ferroxidase center and the stability of rCtFtn. ..
  7. Pedersen M, Pham L, Steensgaard D, Miller M. A reconstituted light-harvesting complex from the green sulfur bacterium Chlorobium tepidum containing CsmA and bacteriochlorophyll a. Biochemistry. 2008;47:1435-41 pubmed publisher
    ..We have reconstituted the isolated CsmA protein with BChl a in micelles of n-octyl beta-d-glucopyranoside. The resulting preparation reproduced the spectral characteristics of the CsmA-BChl a complex present in the chlorosome baseplate...
  8. Shuman K, Hanson T. A sulfide:quinone oxidoreductase from Chlorobaculum tepidum displays unusual kinetic properties. FEMS Microbiol Lett. 2016;363: pubmed publisher
    ..These are the first kinetic data for a type VI SQR and have implications for structure-function analyses of all SQR's. ..
  9. Maresca J, Graham J, Wu M, Eisen J, Bryant D. Identification of a fourth family of lycopene cyclases in photosynthetic bacteria. Proc Natl Acad Sci U S A. 2007;104:11784-9 pubmed publisher
    ..Identification of these cyclases fills a major gap in the carotenoid biosynthetic pathways of green sulfur bacteria and cyanobacteria...
  10. Stout J, De Smet L, Panjikar S, Weiss M, Savvides S, Van Beeumen J. Crystallization, preliminary crystallographic analysis and phasing of the thiosulfate-binding protein SoxY from Chlorobium limicola f. thiosulfatophilum. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006;62:1093-6 pubmed
    ..22, b = 120.11, c = 95.30 A. MIRAS data from Pt(2+)- and Hg(2+)-derivatized SoxY crystals resulted in an interpretable electron-density map at 3 A resolution after density modification. ..
  11. Orf G, Saer R, Niedzwiedzki D, Zhang H, McIntosh C, Schultz J, et al. Evidence for a cysteine-mediated mechanism of excitation energy regulation in a photosynthetic antenna complex. Proc Natl Acad Sci U S A. 2016;113:E4486-93 pubmed publisher
    ..This simple mechanism has implications for the design of bio-inspired light-harvesting antennas and the redesign of natural photosynthetic systems. ..
  12. Supangat S, Seo K, Choi Y, Park Y, Son D, Han C, et al. Structure of Chlorobium tepidum sepiapterin reductase complex reveals the novel substrate binding mode for stereospecific production of L-threo-tetrahydrobiopterin. J Biol Chem. 2006;281:2249-56 pubmed publisher
    ..The different sepiapterin binding mode within the conserved catalytic architecture presents a novel strategy of switching the reaction stereospecificities in the same protein fold...
  13. Deyaert E, Wauters L, Guaitoli G, Konijnenberg A, Leemans M, Terheyden S, et al. A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun. 2017;8:1008 pubmed publisher
    ..This work thus provides insights into the conformational cycle of Roco proteins and suggests a link between oligomerization and disease-associated mutations in LRRK2. ..
  14. Coates L, Beaven G, Erskine P, Beale S, Avissar Y, Gill R, et al. The X-ray structure of the plant like 5-aminolaevulinic acid dehydratase from Chlorobium vibrioforme complexed with the inhibitor laevulinic acid at 2.6 A resolution. J Mol Biol. 2004;342:563-70 pubmed
    ..A different site close to the active site flap, in which a putative magnesium ion is coordinated by a glutamate carboxyl and five solvent molecules may account for the stimulatory properties of magnesium ions on the enzyme. ..
  15. Yoon K, Hille R, Hemann C, Tabita F. Rubredoxin from the green sulfur bacterium Chlorobium tepidum functions as an electron acceptor for pyruvate ferredoxin oxidoreductase. J Biol Chem. 1999;274:29772-8 pubmed
    ..The midpoint reduction potential of C. tepidum Rd was determined to be -87 mV, which is the most electronegative value reported for Rd from any source...
  16. Naterstad K, Lauvrak V, Sirevåg R. Malate dehydrogenase from the mesophile Chlorobium vibrioforme and from the mild thermophile Chlorobium tepidum: molecular cloning, construction of a hybrid, and expression in Escherichia coli. J Bacteriol. 1996;178:7047-52 pubmed
    ..A hybrid mdh was constructed from the 3' part of mdh from C. tepidum and the 5' part of mdh from C. vibrioforme. The thermostabilities of the hybrid enzyme and of MDH from C. vibrioforme and C. tepidum were compared. ..
  17. Hanson T, Tabita F. A ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO)-like protein from Chlorobium tepidum that is involved with sulfur metabolism and the response to oxidative stress. Proc Natl Acad Sci U S A. 2001;98:4397-402 pubmed publisher
    ..This protein might be an evolutional link to bona fide RubisCO and could serve as an important tool to analyze how the RubisCO active site developed...
  18. Chung S, Bryant D. Characterization of csmB genes, encoding a 7.5-kDa protein of the chlorosome envelope, from the green sulfur bacteria Chlorobium vibrioforme 8327D and Chlorobium tepidum. Arch Microbiol. 1996;166:234-44 pubmed
    ..Protease susceptibility mapping and agglutination experiments with isolated chlorosomes using anti-CsmB antibodies indicate that the CsmB protein is a component of the chlorosome envelope. ..
  19. Dalhus B, Saarinen M, Sauer U, Eklund P, Johansson K, Karlsson A, et al. Structural basis for thermophilic protein stability: structures of thermophilic and mesophilic malate dehydrogenases. J Mol Biol. 2002;318:707-21 pubmed publisher
    ..A structural alignment of tetrameric and dimeric prokaryotic MDHs reveal that structural elements that differ among dimeric and tetrameric MDHs are located in a few loop regions...
  20. Ben Shem A, Frolow F, Nelson N. Evolution of photosystem I - from symmetry through pseudo-symmetry to asymmetry. FEBS Lett. 2004;564:274-80 pubmed publisher
  21. Hager Braun C, Xie D, Jarosch U, Herold E, Buttner M, Zimmermann R, et al. Stable photobleaching of P840 in Chlorobium reaction center preparations: presence of the 42-kDa bacteriochlorophyll a protein and a 17-kDa polypeptide. Biochemistry. 1995;34:9617-24 pubmed
    ..42. FMO and PscB show the tendency to form a complementary subcomplex. FMO and PscD are apparently required to stabilize the photoactive reaction center, while the cytochrome c subunit is not. ..
  22. Li H, Sawaya M, Tabita F, Eisenberg D. Crystal structure of a RuBisCO-like protein from the green sulfur bacterium Chlorobium tepidum. Structure. 2005;13:779-89 pubmed publisher
    ..Bioinformatic analysis of the protein functional linkages suggests that this RLP coevolved with enzymes of the bacteriochlorophyll biosynthesis pathway and may be involved in processes related to photosynthesis...
  23. Højrup P, Gerola P, Hansen H, Mikkelsen J, Shahed A, Knudsen J, et al. The amino acid sequence of a major protein component in the light harvesting complex of the green photosynthetic bacterium Chlorobium limicola f. thiosulfatophilum. Biochim Biophys Acta. 1991;1077:220-4 pubmed
    ..The possible role of the protein in the structure and function of the chlorosome is discussed. ..
  24. Milks K, Danielsen M, Persson S, Jensen O, Cox R, Miller M. Chlorosome proteins studied by MALDI-TOF-MS: topology of CsmA in Chlorobium tepidum. Photosynth Res. 2005;86:113-21 pubmed
    ..These results indicate that the N-terminal portion of the CsmA protein, which is predicted to be mainly hydrophobic, is buried in the chlorosome envelope...
  25. Charnock C, Refseth U, Sirevåg R. Malate dehydrogenase from Chlorobium vibrioforme, Chlorobium tepidum, and Heliobacterium gestii: purification, characterization, and investigation of dinucleotide binding by dehydrogenases by use of empirical methods of protein sequence analysis. J Bacteriol. 1992;174:1307-13 pubmed
    ..The sequences presented probably possess a stretch of amino acids involved in dinucleotide binding which is similar to that of Chloroflexus aurantiacus MDH and other classes of dehydrogenase enzymes but unique among MDHs. ..
  26. Saga Y, Hirota K, Harada J, Tamiaki H. In Vitro Enzymatic Activities of Bacteriochlorophyll a Synthase Derived from the Green Sulfur Photosynthetic Bacterium Chlorobaculum tepidum. Biochemistry. 2015;54:4998-5005 pubmed publisher
    ..tepidum. The enzymatic activity of tepBchG was higher than that of BchG of Rhodobacter sphaeroides at 45 °C, although the former activity was lower than the latter below 35 °C. ..
  27. Lange C, Kiesel S, Peters S, Virus S, Scheer H, Jahn D, et al. Broadened Substrate Specificity of 3-Hydroxyethyl Bacteriochlorophyllide a Dehydrogenase (BchC) Indicates a New Route for the Biosynthesis of Bacteriochlorophyll a. J Biol Chem. 2015;290:19697-709 pubmed publisher
    ..Based on these results, we are designating a new medium-chain dehydrogenase/reductase family (MDR057 BchC) as theoretically proposed from a recent bioinformatics analysis. ..
  28. B ttner M, Xie D, Nelson H, Pinther W, Hauska G, Nelson N. Photosynthetic reaction center genes in green sulfur bacteria and in photosystem 1 are related. Proc Natl Acad Sci U S A. 1992;89:8135-9 pubmed
    ..An adjacent third gene, not belonging to the reaction center, encodes a protein related to dolichyl-phosphate-D-mannose synthase from yeast. The different origins of PS1 and PS2 are discussed...
  29. Huang R, Wen J, Blankenship R, Gross M. Hydrogen-deuterium exchange mass spectrometry reveals the interaction of Fenna-Matthews-Olson protein and chlorosome CsmA protein. Biochemistry. 2012;51:187-93 pubmed publisher
    ..The results indicate that the CsmA protein interacts with the Bchl a #1 side of the FMO protein. A global picture including peptide-level details for the architecture of the photosystem from green-sulfur bacteria can now be drawn...
  30. Gruber T, Eisen J, Gish K, Bryant D. The phylogenetic relationships of Chlorobium tepidum and Chloroflexus aurantiacus based upon their RecA sequences. FEMS Microbiol Lett. 1998;162:53-60 pubmed
    ..Cf. aurantiacus was placed near Chlamydia trachomatis and the high-GC Gram-positives; however, this branching pattern was not strongly supported statistically by bootstrap analyses. Possible reasons for this ambiguity are discussed...
  31. Camara Artigas A, Blankenship R, Allen J. The structure of the FMO protein from Chlorobium tepidum at 2.2 A resolution. Photosynth Res. 2003;75:49-55 pubmed
  32. Azai C, Tsukatani Y, Harada J, Oh oka H. Sulfur oxidation in mutants of the photosynthetic green sulfur bacterium Chlorobium tepidum devoid of cytochrome c-554 and SoxB. Photosynth Res. 2009;100:57-65 pubmed publisher
    ..Cytochrome c-554 might function in any other pathway(s) as well as the thiosulfate oxidation one, since even green sulfur bacteria that cannot oxidize thiosulfate contain a cycA gene encoding this electron carrier...
  33. Ratcliff K, Corn J, Marqusee S. Structure, stability, and folding of ribonuclease H1 from the moderately thermophilic Chlorobium tepidum: comparison with thermophilic and mesophilic homologues. Biochemistry. 2009;48:5890-8 pubmed publisher
    ..It should now be possible to design RNases H that display the desired thermophilic or mesophilic properties, as defined by their DeltaC(p) values, and therefore fine-tune the energy landscape in a predictable fashion...
  34. Ogawa T, Furusawa T, Nomura R, Seo D, Hosoya Matsuda N, Sakurai H, et al. SoxAX binding protein, a novel component of the thiosulfate-oxidizing multienzyme system in the green sulfur bacterium Chlorobium tepidum. J Bacteriol. 2008;190:6097-110 pubmed publisher
    ..Each of the deduced SoxA and SoxX proteins of these bacteria constitute groups that are distinct from those found in bacteria that apparently lack SAXB gene homologues...
  35. Meyer S, Scrima A, Vers es W, Wittinghofer A. Crystal structures of the conserved tRNA-modifying enzyme GidA: implications for its interaction with MnmE and substrate. J Mol Biol. 2008;380:532-47 pubmed publisher
    ..We propose a model for the interaction between GidA and MnmE, which is supported by site-directed mutagenesis. Our data suggest that this interaction is modulated and potentially regulated by the switch function of the G domain of MnmE...
  36. Tsukatani Y, Miyamoto R, Itoh S, Oh oka H. Function of a PscD subunit in a homodimeric reaction center complex of the photosynthetic green sulfur bacterium Chlorobium tepidum studied by insertional gene inactivation. Regulation of energy transfer and ferredoxin-mediated NADP+ reduction on the. J Biol Chem. 2004;279:51122-30 pubmed publisher
    ..The evolutionary relationship between PscD and PsaD was also discussed based on a structural homology modeling of the former...
  37. Gomez Maqueo Chew A, Frigaard N, Bryant D. Bacteriochlorophyllide c C-8(2) and C-12(1) methyltransferases are essential for adaptation to low light in Chlorobaculum tepidum. J Bacteriol. 2007;189:6176-84 pubmed publisher
    ..tepidum to low light intensity. The data additionally suggest that these methylations also directly or indirectly affect the regulation of the BChl c biosynthetic pathway...
  38. Wada K, Harada J, Yaeda Y, Tamiaki H, Oh oka H, Fukuyama K. Crystal structures of CbiL, a methyltransferase involved in anaerobic vitamin B biosynthesis, and CbiL in complex with S-adenosylhomocysteine--implications for the reaction mechanism. FEBS J. 2007;274:563-73 pubmed
    ..Furthermore, the structural model of CbiL binding to its substrate suggests the axial residue coordinated to the central cobalt of cobalt-factor II...
  39. Wada K, Yamaguchi H, Harada J, Niimi K, Osumi S, Saga Y, et al. Crystal structures of BchU, a methyltransferase involved in bacteriochlorophyll c biosynthesis, and its complex with S-adenosylhomocysteine: implications for reaction mechanism. J Mol Biol. 2006;360:839-49 pubmed publisher
  40. Harada J, Saga Y, Oh oka H, Tamiaki H. Different sensitivities to oxygen between two strains of the photosynthetic green sulfur bacterium Chlorobium vibrioforme NCIB 8327 with bacteriochlorophyll c and d. Photosynth Res. 2005;86:137-43 pubmed publisher
    ..The result would be ascribable to the difference of the midpoint potentials between two kinds of chlorosomes formed by self-aggregates of BChl c and d as measured by their fluorescence quenching...
  41. Coates L, Beaven G, Erskine P, Beale S, Wood S, Shoolingin Jordan P, et al. Structure of Chlorobium vibrioforme 5-aminolaevulinic acid dehydratase complexed with a diacid inhibitor. Acta Crystallogr D Biol Crystallogr. 2005;61:1594-8 pubmed
    ..The structure suggests why 4,7-dioxosebacic acid is a better inhibitor of the zinc-dependent ALADs than of the zinc-independent ALADs. ..