Neisseria gonorrhoeae FA 1090

Summary

Alias: Neisseria gonorrhoeae FA1090, Neisseria gonorrhoeae str. FA 1090, Neisseria gonorrhoeae strain FA 1090

Top Publications

  1. Connell T, Black W, Kawula T, Barritt D, Dempsey J, Kverneland K, et al. Recombination among protein II genes of Neisseria gonorrhoeae generates new coding sequences and increases structural variability in the protein II family. Mol Microbiol. 1988;2:227-36 pubmed
    ..Moreover, we found that reassortment of the two HV regions among P.II genes occurs, generating increased structural and antigenic variability in the P.II protein family. ..
  2. Freitag N, Seifert H, Koomey M. Characterization of the pilF-pilD pilus-assembly locus of Neisseria gonorrhoeae. Mol Microbiol. 1995;16:575-86 pubmed
    ..Although expression of orfX and pilD appears to be transcriptionally coupled, mutants containing transposon insertions in orfX expressed pili. Unlike either pilF or pilD mutants, orfX mutants were also competent for DNA transformation. ..
  3. Black C, Fyfe J, Davies J. A promoter associated with the neisserial repeat can be used to transcribe the uvrB gene from Neisseria gonorrhoeae. J Bacteriol. 1995;177:1952-8 pubmed
    ..A corresponding sigma 70 promoter was identified within a copy of the 26-bp neisserial repeat, and this identification provided the first evidence of a promoter associated with this repetitive element in N. gonorrhoeae. ..
  4. Gunn J, Stein D. Use of a non-selective transformation technique to construct a multiply restriction/modification-deficient mutant of Neisseria gonorrhoeae. Mol Gen Genet. 1996;251:509-17 pubmed
    ..The development of a simple, non-selective transformation technique, coupled with the construction of a strain that is more permissive for DNA-mediated transformation, will aid in genetic manipulations of the gonococcus. ..
  5. Banerjee A, Wang R, Uljon S, Rice P, Gotschlich E, Stein D. Identification of the gene (lgtG) encoding the lipooligosaccharide beta chain synthesizing glucosyl transferase from Neisseria gonorrhoeae. Proc Natl Acad Sci U S A. 1998;95:10872-7 pubmed
    ..These results show that, like the alpha chain, the beta chain of lipooligosaccharide is subject to antigenic variation. ..
  6. Mehr I, Seifert H. Differential roles of homologous recombination pathways in Neisseria gonorrhoeae pilin antigenic variation, DNA transformation and DNA repair. Mol Microbiol. 1998;30:697-710 pubmed
    ..In addition, mutations in the Gc recB, recC or recD genes revealed that a Gc RecBCD pathway also exists and is involved in DNA transformation and DNA repair but not in pilin antigenic variation. ..
  7. Saunders N, Snyder L. The minimal mobile element. Microbiology. 2002;148:3756-60 pubmed
  8. Potter A, Kidd S, Jennings M, McEwan A. Evidence for distinctive mechanisms of S-nitrosoglutathione metabolism by AdhC in two closely related species, Neisseria gonorrhoeae and Neisseria meningitidis. Infect Immun. 2007;75:1534-6 pubmed
    ..Mutational analysis showed that NADH S-nitrosoglutathione oxidoreductase activity was associated with adhC in Neisseria meningitidis but not in Neisseria gonorrhoeae. ..
  9. Elkins C, Thomas C, Seifert H, Sparling P. Species-specific uptake of DNA by gonococci is mediated by a 10-base-pair sequence. J Bacteriol. 1991;173:3911-3 pubmed
    ..We show that the presence of various forms of this 10-bp sequence results in increased uptake of double-stranded DNA into a DNase-resistant state and allows genetic transformation by an otherwise nontransformable plasmid. ..

More Information

Publications150 found, 100 shown here

  1. Carbonetti N, Simnad V, Seifert H, So M, Sparling P. Genetics of protein I of Neisseria gonorrhoeae: construction of hybrid porins. Proc Natl Acad Sci U S A. 1988;85:6841-5 pubmed
  2. Banerjee A, Wang R, Supernavage S, Ghosh S, Parker J, Ganesh N, et al. Implications of phase variation of a gene (pgtA) encoding a pilin galactosyl transferase in gonococcal pathogenesis. J Exp Med. 2002;196:147-62 pubmed
    ..In contrast, for the vast majority (20 out of 28) of GC isolated from uncomplicated gonorrhea (UG) patients, pgtA lacked the poly-G. These results indicate that Pv of pgtA is likely to be involved in the conversion of UG to DGI. ..
  3. Dong J, George N, Duckett K, DeBeer M, LOPPER M. The crystal structure of Neisseria gonorrhoeae PriB reveals mechanistic differences among bacterial DNA replication restart pathways. Nucleic Acids Res. 2010;38:499-509 pubmed publisher
    ..These results provide evidence that mechanisms of DNA replication restart are not identical across diverse species and that these pathways have likely become specialized to meet the needs of individual organisms. ..
  4. Hoehn G, Clark V. The major anaerobically induced outer membrane protein of Neisseria gonorrhoeae, Pan 1, is a lipoprotein. Infect Immun. 1992;60:4704-8 pubmed
    ..3H]palmitic acid labeling of gonococci grown under oxygen-limited conditions demonstrated specific incorporation of label into Pan 1, suggesting further that Pan 1 is a lipoprotein...
  5. Correia F, Inouye S, Inouye M. A family of small repeated elements with some transposon-like properties in the genome of Neisseria gonorrhoeae. J Biol Chem. 1988;263:12194-8 pubmed
    ..In one instance two copies of the 152-bp repetitive sequence are separated by a 436-bp central region and are in an inverted orientation with respect to one another, resembling a compound transposable element...
  6. Gotschlich E. Genetic locus for the biosynthesis of the variable portion of Neisseria gonorrhoeae lipooligosaccharide. J Exp Med. 1994;180:2181-90 pubmed
    ..It is likely that these structural features are responsible for the high-frequency genetic variation of gonococcal LOS...
  7. Zhou J, Bowler L, Spratt B. Interspecies recombination, and phylogenetic distortions, within the glutamine synthetase and shikimate dehydrogenase genes of Neisseria meningitidis and commensal Neisseria species. Mol Microbiol. 1997;23:799-812 pubmed
  8. Mellies J, Jose J, Meyer T. The Neisseria gonorrhoeae gene aniA encodes an inducible nitrite reductase. Mol Gen Genet. 1997;256:525-32 pubmed
    ..Finally, we report investigations to determine whether AniA protein is involved in gonococcal pathogenesis...
  9. Jennings M, Virji M, Evans D, Foster V, Srikhanta Y, Steeghs L, et al. Identification of a novel gene involved in pilin glycosylation in Neisseria meningitidis. Mol Microbiol. 1998;29:975-84 pubmed
  10. Boulanger M, Murphy M. Crystal structure of the soluble domain of the major anaerobically induced outer membrane protein (AniA) from pathogenic Neisseria: a new class of copper-containing nitrite reductases. J Mol Biol. 2002;315:1111-27 pubmed publisher
    ..A unique combination of structural effectors surrounding the type I copper site of sAnia contribute to a unusual visible absorption spectra with components observed previously in either green or blue type I copper sites...
  11. Kahler C, Blum E, Miller Y, Ryan D, Popovic T, Stephens D. exl, an exchangeable genetic island in Neisseria meningitidis. Infect Immun. 2001;69:1687-96 pubmed publisher
    ..The structure of these cassettes and their correlation with clonal groups emphasize the extensive gene pool and frequent horizontal DNA transfer events that contribute to the evolution and virulence of N. meningitidis...
  12. Berish S, Mietzner T, Mayer L, Genco C, Holloway B, Morse S. Molecular cloning and characterization of the structural gene for the major iron-regulated protein expressed by Neisseria gonorrhoeae. J Exp Med. 1990;171:1535-46 pubmed
    ..These results complement the biochemical characterization of the Fbp expressed by gonococci and further suggest that it has a role in iron-acquisition. ..
  13. Ropp P, Nicholas R. Cloning and characterization of the ponA gene encoding penicillin-binding protein 1 from Neisseria gonorrhoeae and Neisseria meningitidis. J Bacteriol. 1997;179:2783-7 pubmed
  14. Garcia D, Dillard J. Mutations in ampG or ampD affect peptidoglycan fragment release from Neisseria gonorrhoeae. J Bacteriol. 2008;190:3799-807 pubmed publisher
    ..These results demonstrate that N. gonorrhoeae has an active peptidoglycan recycling pathway and can regulate peptidoglycan fragment metabolism, dependent on the intracellular concentration of peptidoglycan fragments...
  15. Folster J, Dhulipala V, Nicholas R, Shafer W. Differential regulation of ponA and pilMNOPQ expression by the MtrR transcriptional regulatory protein in Neisseria gonorrhoeae. J Bacteriol. 2007;189:4569-77 pubmed publisher
  16. Friedrich A, ARVIDSON C, Shafer W, Lee E, So M. Two ABC transporter operons and the antimicrobial resistance gene mtrF are pilT responsive in Neisseria gonorrhoeae. J Bacteriol. 2007;189:5399-402 pubmed
    ..This effect occurs indirectly through the transcriptional regulator FarR, which until now has been shown to regulate only farAB. L-Glutamine can reverse pilT downregulation of the ABC transporter operons and mtrF...
  17. Shi D, Sagar V, Jin Z, Yu X, Caldovic L, Morizono H, et al. The crystal structure of N-acetyl-L-glutamate synthase from Neisseria gonorrhoeae provides insights into mechanisms of catalysis and regulation. J Biol Chem. 2008;283:7176-84 pubmed publisher
    ..The AAK domain, although catalytically inactive, appears to bind arginine. This is the first reported crystal structure of any NAGS, and it provides insights into the catalytic function and arginine regulation of NAGS enzymes...
  18. Ullrich J, van Putten J. Identification of the gonococcal glmU gene encoding the enzyme N-acetylglucosamine 1-phosphate uridyltransferase involved in the synthesis of UDP-GlcNAc. J Bacteriol. 1995;177:6902-9 pubmed
  19. Cooke S, Jolley K, Ison C, Young H, Heckels J. Naturally occurring isolates of Neisseria gonorrhoeae, which display anomalous serovar properties, express PIA/PIB hybrid porins, deletions in PIB or novel PIA molecules. FEMS Microbiol Lett. 1998;162:75-82 pubmed
    ..This is the first demonstration of naturally occurring gonococci expressing a hybrid Protein IA/IB...
  20. Ropp P, Nicholas R. Nucleotide sequence of a three gene cluster in Neisseria Gonorrhoeae encoding ribosomal proteins S6, S18, and L9. DNA Seq. 1998;9:341-5 pubmed
    ..The putative amino acid sequences deduced from all three genes show a high degree of homology to other bacterial ribosomal proteins. ..
  21. Masters T, Wachter J, Hill S. Loop structures in the 5' untranslated region and antisense RNA mediate pilE gene expression in Neisseria gonorrhoeae. Microbiology. 2016;162:2005-2016 pubmed publisher
    ..Overall, this study demonstrates a complex relationship between loop-dependent transcript protection and antisense RNA in modulating pilE expression levels. ..
  22. Pannekoek Y, van Putten J, Dankert J. Identification and molecular analysis of a 63-kilodalton stress protein from Neisseria gonorrhoeae. J Bacteriol. 1992;174:6928-37 pubmed
    ..This relationship was further substantiated by the immunological cross-reactivity of GSP63 with mycobacterial Hsp60 and the ATP-binding activity of the gonococcal stress protein...
  23. Butt N, Virji M, Vayreda F, Lambden P, Heckels J. Gonococcal outer-membrane protein PIB: comparative sequence analysis and localization of epitopes which are recognized by type-specific and cross-reacting monoclonal antibodies. J Gen Microbiol. 1990;136:2165-72 pubmed publisher
    ..However, the conserved epitope recognized by mAb SM24 is centred on residues 198-199, close to Var1 , and is exposed for bactericidal killing...
  24. Aspholm M, Aas F, Harrison O, Quinn D, Vik A, Viburiene R, et al. Structural alterations in a component of cytochrome c oxidase and molecular evolution of pathogenic Neisseria in humans. PLoS Pathog. 2010;6:e1001055 pubmed publisher
    ..They also show how evolutionary change at the molecular level can be linked to metabolic innovation and its reversal as well as demonstrating how genotype can be used to infer alterations of the fitness landscape within a single host. ..
  25. Baehr W, Gotschlich E, Hitchcock P. The virulence-associated gonococcal H.8 gene encodes 14 tandemly repeated pentapeptides. Mol Microbiol. 1989;3:49-55 pubmed
    ..Furthermore, DNA encoding this repeat appears to have been duplicated and translocated into another neisserial gene encoding an azurin...
  26. Martin D, Cadieux N, Hamel J, Brodeur B. Highly conserved Neisseria meningitidis surface protein confers protection against experimental infection. J Exp Med. 1997;185:1173-83 pubmed
    ..The fact that the NspA protein can elicit the production of bactericidal and protective antibodies emphasize its potential as a vaccine candidate...
  27. Salimnia H, Radia A, Bernatchez S, Beveridge T, Dillon J. Characterization of the ftsZ cell division gene of Neisseria gonorrhoeae: expression in Escherichia coli and N. gonorrhoeae. Arch Microbiol. 2000;173:10-20 pubmed
    ..Expression of the FtsZ(Ng)-GFP fusion protein in N. gonorrhoeae resulted in abnormal cell division as shown by electron microscopy. FtsZ(Ng)-GFP fusions were also expressed in a gonococcal background using a unique shuttle vector...
  28. Das D, Grishin N, Kumar A, Carlton D, Bakolitsa C, Miller M, et al. The structure of the first representative of Pfam family PF09836 reveals a two-domain organization and suggests involvement in transcriptional regulation. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010;66:1174-81 pubmed publisher
    ..As these are important human pathogens, NGO1945 represents an interesting candidate for further exploration via biochemical studies and possible therapeutic intervention. ..
  29. Zhu P, Morelli G, Achtman M. The opcA and (psi)opcB regions in Neisseria: genes, pseudogenes, deletions, insertion elements and DNA islands. Mol Microbiol. 1999;33:635-50 pubmed
    ..DNA islands in Nm and Ng seem to have been acquired by recombination via conserved flanking housekeeping genes rather than by insertion of mobile genetic elements...
  30. Zhu P, Tsai C, Frasch C. Immunologic and genetic characterization of lipooligosaccharide variants in a Neisseria meningitidis serogroup C strain. FEMS Immunol Med Microbiol. 2002;34:193-200 pubmed
    ..These findings suggest that besides phase variation, homologous recombination can contribute to the genetic diversity of the lgt locus and to the generation of LOS variation in N. meningitidis. ..
  31. Zhu P, Klutch M, Bash M, Tsang R, Ng L, Tsai C. Genetic diversity of three lgt loci for biosynthesis of lipooligosaccharide (LOS) in Neisseria species. Microbiology. 2002;148:1833-44 pubmed publisher
    ..The data demonstrated that pathogenic and commensal Neisseria share a common lgt gene pool and horizontal gene transfer appears to contribute to the genetic diversity of the lgt loci in Neisseria...
  32. Robertson B, Frosch M, van Putten J. The identification of cryptic rhamnose biosynthesis genes in Neisseria gonorrhoeae and their relationship to lipopolysaccharide biosynthesis. J Bacteriol. 1994;176:6915-20 pubmed
    ..gonorrhoeae either because of the presence of the galE gene or because of another as yet unrecognized function...
  33. Stein D, Chien R, Seifert H. Construction of a Neisseria gonorrhoeae MS11 derivative deficient in NgoMI restriction and modification. J Bacteriol. 1992;174:4899-906 pubmed
    ..Transformation data indicated that MS11 does not produce enough restriction activity to block plasmid transformation in the gonococcus, even though restriction activity could be demonstrated in E. coli containing the cloned gene. ..
  34. Cornelissen C, Biswas G, Tsai J, Paruchuri D, Thompson S, Sparling P. Gonococcal transferrin-binding protein 1 is required for transferrin utilization and is homologous to TonB-dependent outer membrane receptors. J Bacteriol. 1992;174:5788-97 pubmed
    ..Thus, both the function and the predicted protein sequence of TBP1 were consistent with this protein serving as a transferrin receptor...
  35. Spratt B, Zhou J, Taylor M, Merrick M. Monofunctional biosynthetic peptidoglycan transglycosylases. Mol Microbiol. 1996;19:639-40 pubmed
  36. Arvidson C, Powers T, Walter P, So M. Neisseria gonorrhoeae PilA is an FtsY homolog. J Bacteriol. 1999;181:731-9 pubmed
    ..Taken together, these results show that the gonococcal PilA is an FtsY homolog and that the GTPase activity is necessary for its function. ..
  37. K llstr m H, Jonsson A. Characterization of the region downstream of the pilus biogenesis gene pilC1 in Neisseria gonorrhoeae. Biochim Biophys Acta. 1998;1397:137-40 pubmed
    ..PCR with primers specific for ORF1 revealed that the gene is present in all gonococcal strains tested. The other open reading frame, ORF2, is highly homologous to the putative integral membrane protein HI1680 of Haemophilus influenzae...
  38. Fyfe J, Davies J. Nucleotide sequence and expression in Escherichia coli of the recA gene of Neisseria gonorrhoeae. Gene. 1990;93:151-6 pubmed
    ..The product of this gene can act as a recombinase in Escherichia coli, but does so with a decreased efficiency, probably because of the formation of mixed multimers with the equivalent E. coli protein...
  39. Martin P, Cooperider J, Mulks M. Sequence of the argF gene encoding ornithine transcarbamoylase from Neisseria gonorrhoeae. Gene. 1990;94:139-40 pubmed
    ..In the flanking regions of the gene were found 15-bp inverted repeats that may serve as transcriptional termination signals, and which contain the neisserial DNA-uptake sequence. ..
  40. Lawson F, Billowes F, Dillon J. Organization of carbamoyl-phosphate synthase genes in Neisseria gonorrhoeae includes a large, variable intergenic sequence which is also present in other Neisseria species. Microbiology. 1995;141 ( Pt 5):1183-91 pubmed
    ..meningitidis and 18 commensal Neisseria isolates comprising nine species. This unexpected organization of the CPS genes in N. gonorrhoeae is therefore widespread throughout the genus Neisseria. ..
  41. Chaussee M, Wilson J, Hill S. Characterization of the recD gene of Neisseria gonorrhoeae MS11 and the effect of recD inactivation on pilin variation and DNA transformation. Microbiology. 1999;145 ( Pt 2):389-400 pubmed publisher
    ..The nucleotide changes at pilE in the recD mutants were confined to the variable regions of the gene and were similar to changes previously attributed to gene conversion...
  42. Arking D, Tong Y, Stein D. Analysis of lipooligosaccharide biosynthesis in the Neisseriaceae. J Bacteriol. 2001;183:934-41 pubmed publisher
    ..This suggests the existence of an additional regulatory mechanism that is responsible for the limited expression of these genes in this strain...
  43. Goh L, Barkham T, Sim T. Molecular cloning and functional characterization of fumarases C in Neisseria species. Antonie Van Leeuwenhoek. 2005;87:205-13 pubmed publisher
    ..Whether FUMC has any physiological relevance to the pathogenesisity of Neisseriae must await future gene disruption or mutagenesis studies...
  44. Isabella V, Clark V. Identification of a conserved protein involved in anaerobic unsaturated fatty acid synthesis in Neiserria gonorrhoeae: implications for facultative and obligate anaerobes that lack FabA. Mol Microbiol. 2011;82:489-501 pubmed publisher
  45. Rotman E, Seifert H. Neisseria gonorrhoeae MutS affects pilin antigenic variation through mismatch correction and not by pilE guanine quartet binding. J Bacteriol. 2015;197:1828-38 pubmed publisher
  46. Stein D, Levan A, Hardy B, Wang L, Zimmerman L, Song W. Expression of Opacity Proteins Interferes with the Transmigration of Neisseria gonorrhoeae across Polarized Epithelial Cells. PLoS ONE. 2015;10:e0134342 pubmed publisher
    ..Our results suggest that Opa expression limits gonococcal ability to invade into subepithelial tissues by forming tight interactions with neighboring bacteria and by inducing CEACAM redistribution to cell junctions. ..
  47. Lavitola A, Vanni M, Martin P, Bruni C. Cloning and characterization of a Neisseria gene homologous to hisJ and argT of Escherichia coli and Salmonella typhimurium. Res Microbiol. 1992;143:295-305 pubmed
    ..The gene is transcribed as a monocistronic mRNA species of about 960 nucleotides flanked by regulatory elements for initiation and termination of transcription that are efficiently recognized in an E. coli host. ..
  48. Sullivan K, Saunders J. Nucleotide sequence and genetic organization of the NgoPII restriction-modification system of Neisseria gonorrhoeae. Mol Gen Genet. 1989;216:380-7 pubmed
    ..This overlap may have implications for the regulation of the NgoPII restriction-modification system...
  49. Gulati S, Cox A, Lewis L, Michael F, Li J, Boden R, et al. Enhanced factor H binding to sialylated Gonococci is restricted to the sialylated lacto-N-neotetraose lipooligosaccharide species: implications for serum resistance and evidence for a bifunctional lipooligosaccharide sialyltransferase in Gonococci. Infect Immun. 2005;73:7390-7 pubmed publisher
    ..Thus, serum resistance mediated by sialylation of gonococcal L1 and LNT LOS occurs by different mechanisms, and specificity of factor H binding to sialylated gonococci is restricted to the LNT LOS species...
  50. Criss A, Bonney K, Chang R, Duffin P, LeCuyer B, Seifert H. Mismatch correction modulates mutation frequency and pilus phase and antigenic variation in Neisseria gonorrhoeae. J Bacteriol. 2010;192:316-25 pubmed publisher
    ..The differential effects of MMC and uvrD in gonococci unexpectedly reveal that MMC can function independently of uvrD in this human-specific pathogen...
  51. Hoehn G, Clark V. Isolation and nucleotide sequence of the gene (aniA) encoding the major anaerobically induced outer membrane protein of Neisseria gonorrhoeae. Infect Immun. 1992;60:4695-703 pubmed
  52. Cadieux N, Plante M, Rioux C, Hamel J, Brodeur B, Martin D. Bactericidal and cross-protective activities of a monoclonal antibody directed against Neisseria meningitidis NspA outer membrane protein. Infect Immun. 1999;67:4955-9 pubmed
    ..The data presented in this study suggest that antibodies directed against this highly conserved outer membrane protein could protect against meningococcal infections...
  53. Limnios A, Tapsall J, Kahlmeter J, Hogan T, Ray S, Lam A, et al. Cefpodoxime 10 ?g disc screening test for detection of Neisseria gonorrhoeae with mosaic PBP2 and decreased susceptibility to extended-spectrum cephalosporins for public health purposes. APMIS. 2011;119:356-63 pubmed publisher
    ..This test can be used in AMR surveillance programmes for public health purposes especially in less-resourced settings. Further studies to refine this disc testing-based approach are in progress. ..
  54. Feng C, Sunchu B, Greenwood M, LOPPER M. A bacterial PriB with weak single-stranded DNA binding activity can stimulate the DNA unwinding activity of its cognate PriA helicase. BMC Microbiol. 2011;11:189 pubmed publisher
    ..Thus, the weak interaction between N. gonorrhoeae PriB and DNA might be compensated for by the strong interaction between PriB and PriA, which could result in allosteric activation of PriA's ATPase activity. ..
  55. Adhikari P, Berish S, Nowalk A, Veraldi K, Morse S, Mietzner T. The fbpABC locus of Neisseria gonorrhoeae functions in the periplasm-to-cytosol transport of iron. J Bacteriol. 1996;178:2145-9 pubmed
    ..The fpbABC operon differs from these loci in that it is lethal when overexpressed in Escherichia coli...
  56. Hill S, Belland R, Wilson J. The ihf mRNA levels decline as Neisseria gonorrhoeae enters the stationary growth phase. Gene. 1998;215:303-10 pubmed
    ..Overall, this study reveals several unique features of ihf transcription in the gonococcus which questions whether certain aspects if ihf transcriptional regulation are universally shared by all Gram-negative bacteria. ..
  57. Hopper S, Wilbur J, Vasquez B, Larson J, Clary S, Mehr I, et al. Isolation of Neisseria gonorrhoeae mutants that show enhanced trafficking across polarized T84 epithelial monolayers. Infect Immun. 2000;68:896-905 pubmed
    ..In contrast, growth of this mutant in liquid media is normal. Taken together, these results strongly suggest that traversal of N. gonorrhoeae across an epithelial barrier is linked to intracellular bacterial growth...
  58. Tseng H, McEwan A, Apicella M, Jennings M. OxyR acts as a repressor of catalase expression in Neisseria gonorrhoeae. Infect Immun. 2003;71:550-6 pubmed
    ..These data are consistent with OxyR in N. gonorrhoeae acting as a repressor of catalase expression...
  59. Schook P, Stohl E, Criss A, Seifert H. The DNA-binding activity of the Neisseria gonorrhoeae LexA orthologue NG1427 is modulated by oxidation. Mol Microbiol. 2011;79:846-60 pubmed publisher
    ..We propose that NG1427 is inactivated independently of RecA by oxidation...
  60. Narayanan S, Beckham S, Davies J, Roujeinikova A. Purification and biochemical characterization of DnaK and its transcriptional activator RpoH from Neisseria gonorrhoeae. Mol Biol Rep. 2014;41:7945-53 pubmed publisher
    ..Sequence comparison showed that the DnaK-binding site of σ(32) is not conserved in RpoH. Our findings suggest that the mechanism of DnaK/RpoH recognition in N. gonorrhoeae is different from that in E. coli. ..
  61. Cash D, Noinaj N, Buchanan S, Cornelissen C. Beyond the Crystal Structure: Insight into the Function and Vaccine Potential of TbpA Expressed by Neisseria gonorrhoeae. Infect Immun. 2015;83:4438-49 pubmed publisher
    ..Our studies demonstrate that while the loop 3 helix is a key functional domain, its function does not exclusively rely on any single residue. ..
  62. Butt N, Lambden P, Heckels J. The nucleotide sequence of the por gene from Neisseria gonorrhoeae strain P9 encoding outer membrane protein PIB. Nucleic Acids Res. 1990;18:4258 pubmed
  63. Thomas C, Sparling P. Identification and cloning of a fur homologue from Neisseria meningitidis. Mol Microbiol. 1994;11:725-37 pubmed
    ..Inability to construct a meningococcal fur mutant suggested that fur may be essential in this species...
  64. Fussenegger M, Facius D, Meier J, Meyer T. A novel peptidoglycan-linked lipoprotein (ComL) that functions in natural transformation competence of Neisseria gonorrhoeae. Mol Microbiol. 1996;19:1095-105 pubmed
    ..Homologous counterparts of the comL gene are found in Neisseria meningitidis as well as in several nonpathogenic Neisseria species...
  65. Arvidson C, So M. Isolation and biochemical characterization of the PilA protein of Neisseria meningitidis. Arch Biochem Biophys. 1997;348:357-62 pubmed
    ..Additionally, the nucleotide specificity of MC PilA differs from that of GC PilA. While GC PilA hydrolyzes only GTP, MC PilA hydrolyzes GTP and ATP equally well, and CTP and UTP also compete for this activity. ..
  66. Barten R, Meyer T. Cloning and characterisation of the Neisseria gonorrhoeae aroB gene. Mol Gen Genet. 1998;258:34-44 pubmed
    ..The aroB gene might be useful as a selectable marker and target for attenuation of a gonococcal live vaccine strain or as a biosafe laboratory strain...
  67. Gruenig M, Stohl E, Chitteni Pattu S, Seifert H, Cox M. Less is more: Neisseria gonorrhoeae RecX protein stimulates recombination by inhibiting RecA. J Biol Chem. 2010;285:37188-97 pubmed publisher
    ..Thus, in N. gonorrhoeae, recombinational processes are facilitated by RecX(Ng) protein-mediated limitations on RecA(Ng) filament presence and/or length to achieve maximal function...
  68. Petricoin E, Danaher R, Stein D. Analysis of the lsi region involved in lipooligosaccharide biosynthesis in Neisseria gonorrhoeae. J Bacteriol. 1991;173:7896-902 pubmed
    ..The mutant was characterized by Southern blotting, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and Western blotting (immunoblotting) and found to be phenotypically identical to FA5100...
  69. Goodman S, Scocca J. Factors influencing the specific interaction of Neisseria gonorrhoeae with transforming DNA. J Bacteriol. 1991;173:5921-3 pubmed
    ..The average frequency of targets in chromosomal DNA of N. gonorrhoeae appears to be approximately one per 1,000 bp...
  70. Woods J, Spinola S, Strobel S, Cannon J. Conserved lipoprotein H.8 of pathogenic Neisseria consists entirely of pentapeptide repeats. Mol Microbiol. 1989;3:43-8 pubmed
    ..The AAEAP units form a repeating epitope that comprises the entire predicted sequence of the protein...
  71. Gotschlich E, Seiff M, Blake M. The DNA sequence of the structural gene of gonococcal protein III and the flanking region containing a repetitive sequence. Homology of protein III with enterobacterial OmpA proteins. J Exp Med. 1987;165:471-82 pubmed
    ..This region contained an exact copy of the consensus sequence of a 26-bp repetitive sequence recently described. An analogous sequence recurs in an inverted orientation 53 bp downstream...
  72. T njum T, Freitag N, Namork E, Koomey M. Identification and characterization of pilG, a highly conserved pilus-assembly gene in pathogenic Neisseria. Mol Microbiol. 1995;16:451-64 pubmed
    ..These findings could not be ascribed to pilin-gene alterations or to polarity exerted on pilD expression. The results indicated that PilG exerts its own independent role in neisserial pilus biogenesis...
  73. Tinsley C, Gotschlich E. Cloning and characterization of the meningococcal polyphosphate kinase gene: production of polyphosphate synthesis mutants. Infect Immun. 1995;63:1624-30 pubmed
    ..The mutants grew less vigorously than wild-type organisms in vitro and showed a striking increase in sensitivity to killing by human serum...
  74. Facius D, Meyer T. A novel determinant (comA) essential for natural transformation competence in Neisseria gonorrhoeae and the effect of a comA defect on pilin variation. Mol Microbiol. 1993;10:699-712 pubmed
    ..The results are in agreement with previous observations supporting the notion that multiple recombination pathways contribute to the variability of pilE...
  75. Lauer P, Albertson N, Koomey M. Conservation of genes encoding components of a type IV pilus assembly/two-step protein export pathway in Neisseria gonorrhoeae. Mol Microbiol. 1993;8:357-68 pubmed
    ..These results suggest that Neisseria gonorrhoeae is capable of expressing many of the essential elements of a highly conserved protein translocation system and that these gene products are probably involved in pilus biogenesis...
  76. Robertson B, Frosch M, van Putten J. The role of galE in the biosynthesis and function of gonococcal lipopolysaccharide. Mol Microbiol. 1993;8:891-901 pubmed
    ..Furthermore, infection experiments in vitro indicate that the galE mutants exhibit unaltered intergonococcal adhesion as well as adhesion to, and invasion of, epithelial cells...
  77. Mehr I, Long C, Serkin C, Seifert H. A homologue of the recombination-dependent growth gene, rdgC, is involved in gonococcal pilin antigenic variation. Genetics. 2000;154:523-32 pubmed
    ..The requirement of RdgC for efficient pilin variation suggests a role for this protein in specialized DNA recombination reactions...
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    ..This protection appears not to be due to complement-mediated lysis and indicates that serum bactericidal activity may not always be the most appropriate predictor of efficacy for protein-based meningococcal vaccines...
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    ..meningitidis. Sulphonamide resistant commensal Neisseria were isolated from an environment not exposed to sulphonamides, suggesting that resistant Neisseria has become a natural part of the commensal throat flora...
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    ..In N. meningitidis, a dif site is present in this location and also serves as the point of chromosomal integration of the GGI in this species...
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    ..Interactions between two FitB molecules result in the formation of a tetramer of FitAB heterodimers, which binds to the 36-bp DNA fragment and provides an explanation for how FitB enhances the DNA binding affinity of FitA...
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    ..Expression was further modulated by iron availability. To our knowledge, this study is the first to demonstrate the specific requirement for a single iron transporter in the survival of a bacterial pathogen within host epithelial cells...
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    ..We conclude that NER functions are conserved in N. gonorrhoeae and are important for the DNA repair capabilities of this strict human pathogen...
  86. Gotschlich E, Seiff M, Blake M, Koomey M. Porin protein of Neisseria gonorrhoeae: cloning and gene structure. Proc Natl Acad Sci U S A. 1987;84:8135-9 pubmed
    ..Hybridization analysis suggests that there is a single structural gene for PI and that it is homologous to the gene found in a PIA-bearing strain of gonococcus...
  87. Beucher M, Sparling P. Cloning, sequencing, and characterization of the gene encoding FrpB, a major iron-regulated, outer membrane protein of Neisseria gonorrhoeae. J Bacteriol. 1995;177:2041-9 pubmed
    ..While FrpB was related to the family of TonB-dependent proteins, a function in iron uptake could not be documented...
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    ..The frequencies of transformation, resulting in different levels of ciprofloxacin resistance, further support the notion that both gyrA and parC genes are involved in the establishment of extreme levels of ciprofloxacin resistance...
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    ..These comparisons should be extended to other Neisseria strains in order to evaluate further this genetic divergence further...
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    ..In addition, we evaluated a panel of gonococcal strains for antigenic diversity using these antipeptide sera...