Burkholderia xenovorans LB400

Summary

Alias: Burkholderia cepacia LB400, Burkholderia fungorum LB400, Burkholderia sp. LB400, Burkholderia xenovorans str. LB400, Burkholderia xenovorans strain LB400, Pseudomonas LB400, Pseudomonas sp. (strain LB400), Pseudomonas sp. LB400

Top Publications

  1. Mahenthiralingam E, Baldwin A, Vandamme P. Burkholderia cepacia complex infection in patients with cystic fibrosis. J Med Microbiol. 2002;51:533-8 pubmed publisher
    ..For microbiologists, hospital infection control officers, caregivers, and most of all the CF community, the changes in our understanding of the taxonomy, epidemiology and pathogenesis of the bacterium 'B. cepacia' are complex...
  2. Li J, Li C, Blindauer C, Bugg T. Evidence for a gem-diol reaction intermediate in bacterial C-C hydrolase enzymes BphD and MhpC from 13C NMR spectroscopy. Biochemistry. 2006;45:12461-9 pubmed
    ..C-C cleavage of meta-ring fission intermediates on the Escherichia coli phenylpropionic acid and Burkholderia xenovorans LB400 biphenyl degradation pathways and are both members of the alpha/beta-hydrolase family containing a Ser-..
  3. Ruzzini A, Bhowmik S, Yam K, Ghosh S, Bolin J, Eltis L. The lid domain of the MCP hydrolase DxnB2 contributes to the reactivity toward recalcitrant PCB metabolites. Biochemistry. 2013;52:5685-5695 pubmed publisher
    ..is accommodated by a hydrophobic pocket that is larger than the homologous site in BphDLB400 from Burkholderia xenovorans LB400. The planar binding mode observed in the crystalline complex was consistent with the hyper- and ..
  4. Detsika M, Corkill J, Magalhaes M, Glendinning K, Hart C, Winstanley C. Molecular typing of, and distribution of genetic markers among, Burkholderia cepacia complex isolates from Brazil. J Clin Microbiol. 2003;41:4148-53 pubmed
    ..Nine genomovar IIIA isolates from different non-CF patients and clinical sources had identical genotypes, indicating the presence of a common clone. ..
  5. Bains J, Leon R, Boulanger M. Structural and biophysical characterization of BoxC from Burkholderia xenovorans LB400: a novel ring-cleaving enzyme in the crotonase superfamily. J Biol Chem. 2009;284:16377-85 pubmed publisher
    ..The recently discovered benzoate oxidation pathway in Burkholderia xenovorans LB400 encodes a novel such ring-cleaving enzyme, termed BoxC, that catalyzes the conversion of 2,3-dihydro-2,..
  6. Karthikeyan S, Zhao Z, Kao C, Zhou Q, Tao Z, Zhang H, et al. Structural analysis of 1-aminocyclopropane-1-carboxylate deaminase: observation of an aminyl intermediate and identification of Tyr 294 as the active-site nucleophile. Angew Chem Int Ed Engl. 2004;43:3425-9 pubmed
  7. Kerby R, Roberts G. Burkholderia xenovorans RcoM(Bx)-1, a transcriptional regulator system for sensing low and persistent levels of carbon monoxide. J Bacteriol. 2012;194:5803-16 pubmed publisher
    ..Here the RcoM(Bx)-1 protein from Burkholderia xenovorans LB400 was heterologously expressed and then purified in a form with minimal bound CO (~10%) and was found to ..
  8. Chan C, Stead D, Coutts R. Development of a species-specific recA-based PCR test for Burkholderia fungorum. FEMS Microbiol Lett. 2003;224:133-8 pubmed
    ..Using a similar procedure we extended the recA-based PCR assay to identify B. sacchari and B. caledonica, two additional soil-inhabiting Burkholderia spp. ..
  9. Hülsmeyer M, Hecht H, Niefind K, Hofer B, Eltis L, Timmis K, et al. Crystal structure of cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase from a PCB degrader at 2.0 A resolution. Protein Sci. 1998;7:1286-93 pubmed
    ..These studies further suggest that Asn143 is a key determinant of substrate specificity. A two-step reaction mechanism is proposed for cis-dihydrodiol dehydrogenases. ..

More Information

Publications68

  1. van Waasbergen L, Kidambi S, Miller R. Construction of a recA mutant of Burkholderia (formerly Pseudomonas), cepacia. Appl Microbiol Biotechnol. 1998;49:59-65 pubmed
    ..cepacia and a recA mutant will be useful in bioengineering of this species. ..
  2. Leitgeb S, Straganz G, Nidetzky B. Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves beta-diketones. FEBS J. 2009;276:5983-97 pubmed publisher
  3. Storms V, Van Den Vreken N, Coenye T, Mahenthiralingam E, LiPuma J, Gillis M, et al. Polyphasic characterisation of Burkholderia cepacia-like isolates leading to the emended description of Burkholderia pyrrocinia. Syst Appl Microbiol. 2004;27:517-26 pubmed
    ..cepacia complex species thus far comprising one single soil isolate only. An emended description of Burkholderia pyrrocinia is proposed. The taxonomic status of the remaining two isolates requires further analysis. ..
  4. Nagata Y, Matsuda M, Komatsu H, Imura Y, Sawada H, Ohtsubo Y, et al. Organization and localization of the dnaA and dnaK gene regions on the multichromosomal genome of Burkholderia multivorans ATCC 17616. J Biosci Bioeng. 2005;99:603-10 pubmed
    ..Pulsed-field gel electrophoresis and Southern blot analyses indicated that both the dnaA and dnaK gene regions exist as single copies on the 3.4 Mb chromosome. ..
  5. Haddock J, Gibson D. Purification and characterization of the oxygenase component of biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400. J Bacteriol. 1995;177:5834-9 pubmed
    ..ISPBPH is proposed to be the terminal oxygenase component of biphenyl 2,3-dioxygenase where substrate binding and oxidation occur via addition of molecular oxygen and two reducing equivalents. ..
  6. Robinson A, Edwards K, Carr P, Barton J, Ewart G, Ollis D. Structure of the C123S mutant of dienelactone hydrolase (DLH) bound with the PMS moiety of the protease inhibitor phenylmethylsulfonyl fluoride (PMSF). Acta Crystallogr D Biol Crystallogr. 2000;56:1376-84 pubmed
    ..Implications for mechanism and further engineering studies are discussed. ..
  7. Ruff J, Denger K, Cook A. Sulphoacetaldehyde acetyltransferase yields acetyl phosphate: purification from Alcaligenes defragrans and gene clusters in taurine degradation. Biochem J. 2003;369:275-85 pubmed
    ..Different patterns of regulation for the transport and dissimilation of taurine were hypothesized for S. meliloti and B. fungorum. ..
  8. Wang W, Baker P, Seah S. Comparison of two metal-dependent pyruvate aldolases related by convergent evolution: substrate specificity, kinetic mechanism, and substrate channeling. Biochemistry. 2010;49:3774-82 pubmed publisher
    ..Therefore, the BphI-BphJ enzyme complex exhibits unique bidirectionality in substrate channeling and allosteric activation. ..
  9. Bains J, Wulff J, Boulanger M. Investigating terephthalate biodegradation: structural characterization of a putative decarboxylating cis-dihydrodiol dehydrogenase. J Mol Biol. 2012;423:284-93 pubmed publisher
    ..Overall, this study reveals novel structural and mechanistic insights into a decarboxylating cis-dihydrodiol dehydrogenase that mediates one of the two catalytic steps in the biodegradation of the environmental pollutant Tph. ..
  10. Ollis D, Ngai K. Crystallization and preliminary x-ray crystallographic data of dienelactone hydrolase from Pseudomonas sp. B13. J Biol Chem. 1985;260:9818-9 pubmed
    ..The crystals are orthorhombic, the space group being P212121, with unit cell dimensions a = 48.9 A, b = 71.2 A, and c = 77.5 A. There appears to be 1 molecule in the asymmetric unit. ..
  11. Kronen M, Sasikaran J, Berg I. Mesaconase Activity of Class I Fumarase Contributes to Mesaconate Utilization by Burkholderia xenovorans. Appl Environ Microbiol. 2015;81:5632-8 pubmed publisher
    ..xenovorans class I fumarase is in fact a promiscuous fumarase/mesaconase. This promiscuity is physiologically relevant, as it allows the growth of this bacterium on mesaconate as a sole carbon and energy source. ..
  12. Bhowmik S, Horsman G, Bolin J, Eltis L. The molecular basis for inhibition of BphD, a C-C bond hydrolase involved in polychlorinated biphenyls degradation: large 3-substituents prevent tautomerization. J Biol Chem. 2007;282:36377-85 pubmed
    ..3-Cl HOPDA structure, more consistent with hydrolysis occurring via an acyl-enzyme than a gem-diol intermediate. These results provide insight into engineering BphD for PCB degradation. ..
  13. Li C, Li J, Montgomery M, Wood S, Bugg T. Catalytic role for arginine 188 in the C-C hydrolase catalytic mechanism for Escherichia coli MhpC and Burkholderia xenovorans LB400 BphD. Biochemistry. 2006;45:12470-9 pubmed
    ..cleavage of meta-ring fission intermediates on the Escherichia coli phenylpropionic acid pathway and Burkholderia xenovorans LB400 biphenyl degradation pathway, respectively...
  14. Horsman G, Bhowmik S, Seah S, Kumar P, Bolin J, Eltis L. The tautomeric half-reaction of BphD, a C-C bond hydrolase. Kinetic and structural evidence supporting a key role for histidine 265 of the catalytic triad. J Biol Chem. 2007;282:19894-904 pubmed
    BphD of Burkholderia xenovorans LB400 catalyzes an unusual C-C bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to afford benzoic acid and 2-hydroxy-2,4-pentadienoic acid (HPD)...
  15. Kumar P, Mohammadi M, Dhindwal S, Pham T, Bolin J, Sylvestre M. Structural insights into the metabolism of 2-chlorodibenzofuran by an evolved biphenyl dioxygenase. Biochem Biophys Res Commun. 2012;421:757-62 pubmed publisher
    The biphenyl dioxygenase of Burkholderia xenovorans LB400 (BphAE(LB400)) is a Rieske-type oxygenase that catalyzes the stereospecific oxygenation of many heterocyclic aromatics including dibenzofuran...
  16. Carere J, Baker P, Seah S. Investigating the molecular determinants for substrate channeling in BphI-BphJ, an aldolase-dehydrogenase complex from the polychlorinated biphenyls degradation pathway. Biochemistry. 2011;50:8407-16 pubmed publisher
    ..Substitution of His-20 and Tyr-290 in BphI led to significant reductions in aldehyde channeling efficiencies. A mechanism of substrate channeling involving these two gating residues is proposed. ..
  17. Nakazawa T, Kimoto M, Abe M. Cloning, sequencing, and transcriptional analysis of the recA gene of Pseudomonas cepacia. Gene. 1990;94:83-8 pubmed
    ..An SOS box characteristic to LexA-regulated promoters, along with the -10 and -35 consensus sequences, was found in the 5' upstream region of the P. cepacia recA gene. ..
  18. Horsman G, Ke J, Dai S, Seah S, Bolin J, Eltis L. Kinetic and structural insight into the mechanism of BphD, a C-C bond hydrolase from the biphenyl degradation pathway. Biochemistry. 2006;45:11071-86 pubmed
    Kinetic and structural analyses of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) hydrolase from Burkholderia xenovorans LB400 (BphD(LB400)) provide insight into the catalytic mechanism of this unusual serine hydrolase...
  19. Kim H, Liu J, Carr P, Ollis D. Following directed evolution with crystallography: structural changes observed in changing the substrate specificity of dienelactone hydrolase. Acta Crystallogr D Biol Crystallogr. 2005;61:920-31 pubmed
    ..This rotation of the inhibitor caused the displacement of a large section of a loop on one side of the active site. Residues that could stabilize the transition state for the reaction were identified. ..
  20. Karthikeyan S, Zhou Q, Zhao Z, Kao C, Tao Z, Robinson H, et al. Structural analysis of Pseudomonas 1-aminocyclopropane-1-carboxylate deaminase complexes: insight into the mechanism of a unique pyridoxal-5'-phosphate dependent cyclopropane ring-opening reaction. Biochemistry. 2004;43:13328-39 pubmed publisher
    ..The results of mutagenesis studies corroborated the assigned critical role for Tyr294 in the catalysis...
  21. Pathak D, Ollis D. Refined structure of dienelactone hydrolase at 1.8 A. J Mol Biol. 1990;214:497-525 pubmed
    ..This enables nucleophilic attack by the gamma-sulphur to occur on the cyclic ester substrate through a ring-opening reaction...
  22. Baker P, Carere J, Seah S. Probing the molecular basis of substrate specificity, stereospecificity, and catalysis in the class II pyruvate aldolase, BphI. Biochemistry. 2011;50:3559-69 pubmed publisher
    ..In H20A and H20S variants, pH profiles show the dependence of enzyme activity on hydroxide concentration. On the basis of these results, a catalytic mechanism is proposed...
  23. Pathak D, Ngai K, Ollis D. X-ray crystallographic structure of dienelactone hydrolase at 2.8 A. J Mol Biol. 1988;204:435-45 pubmed
    ..It is believed that the enzymic mechanism of dienelactone hydrolase may be similar to that of the thiol and serine proteases...
  24. Marvin K, Kerby R, Youn H, Roberts G, Burstyn J. The transcription regulator RcoM-2 from Burkholderia xenovorans is a cysteine-ligated hemoprotein that undergoes a redox-mediated ligand switch. Biochemistry. 2008;47:9016-28 pubmed publisher
    ..Like EcDos, the weakly bound axial ligand that is displaced is methionine...
  25. Baugh L, Gallagher L, Patrapuvich R, Clifton M, Gardberg A, Edwards T, et al. Combining functional and structural genomics to sample the essential Burkholderia structome. PLoS ONE. 2013;8:e53851 pubmed publisher
    ..All expression clones and proteins created in this study are freely available by request...
  26. Hohenester E, Keller J, Jansonius J. An alkali metal ion size-dependent switch in the active site structure of dialkylglycine decarboxylase. Biochemistry. 1994;33:13561-70 pubmed
    ..In contrast to Na+, the Rb+ ion is bound with unfavorable geometry, and it is proposed that the rigid site 2 structure results in a pronounced selectivity for Na+ ions...
  27. Colbert C, Couture M, Eltis L, Bolin J. A cluster exposed: structure of the Rieske ferredoxin from biphenyl dioxygenase and the redox properties of Rieske Fe-S proteins. Structure. 2000;8:1267-78 pubmed
    ..Moreover, it appears that the redox-linked ionizations of the Rieske proteins from proton-translocating complexes are also promoted by these electrostatic interactions...
  28. Baker P, Pan D, Carere J, Rossi A, Wang W, Seah S. Characterization of an aldolase-dehydrogenase complex that exhibits substrate channeling in the polychlorinated biphenyls degradation pathway. Biochemistry. 2009;48:6551-8 pubmed publisher
    ..and dehydrogenase complex from the polychlorinated biphenyl degradation pathway of the bacterium Burkholderia xenovorans LB400 was purified...
  29. Toney M, Hohenester E, Cowan S, Jansonius J. Dialkylglycine decarboxylase structure: bifunctional active site and alkali metal sites. Science. 1993;261:756-9 pubmed
    ..The other is located at the carboxyl terminus of an alpha helix. These sites help show how proteins can specifically bind alkali metals and how these ions can exert functional effects...
  30. Couture M, Colbert C, Babini E, Rosell F, Mauk A, Bolin J, et al. Characterization of BphF, a Rieske-type ferredoxin with a low reduction potential. Biochemistry. 2001;40:84-92 pubmed
    ..These results indicate that the 450 mV spread in reduction potential of Rieske clusters of dioxygenase-associated ferredoxins and mitochondrial bc(1) complexes is not due to significant differences in their solvent exposure...
  31. Eltis L, Hofmann B, Hecht H, L nsdorf H, Timmis K. Purification and crystallization of 2,3-dihydroxybiphenyl 1,2-dioxygenase. J Biol Chem. 1993;268:2727-32 pubmed
    ..Electron microscopy indicates that the enzyme is an octamer (265 kDa) consisting of subunits arranged in two planar tetramers in a staggered conformation...
  32. H bner A, Hendrickson W. A fusion promoter created by a new insertion sequence, IS1490, activates transcription of 2,4,5-trichlorophenoxyacetic acid catabolic genes in Burkholderia cepacia AC1100. J Bacteriol. 1997;179:2717-23 pubmed
    ..Our results confirm the hypothesis that IS elements play a central role in transcription of 2,4,5-T genes and likely have stimulated rapid evolution of the metabolic pathway...
  33. Vaillancourt F, Han S, Fortin P, Bolin J, Eltis L. Molecular basis for the stabilization and inhibition of 2, 3-dihydroxybiphenyl 1,2-dioxygenase by t-butanol. J Biol Chem. 1998;273:34887-95 pubmed
    ..In contrast, crystal structures of complexes with catechol and 3-methylcatechol revealed relationships between the binding of these smaller substrates and t-butanol that are consistent with the observed competitive inhibition...
  34. Dai S, Vaillancourt F, Maaroufi H, Drouin N, Neau D, Snieckus V, et al. Identification and analysis of a bottleneck in PCB biodegradation. Nat Struct Biol. 2002;9:934-9 pubmed publisher
    ..Crystal structures of two complexes of DHBD with ortho-chlorinated metabolites at 1.7 A resolution reveal an explanation for these phenomena, which have important implications for bioremediation strategies...
  35. Maity T, Close D, Valdez Y, Nowak Lovato K, Marti Arbona R, Nguyen T, et al. Discovery of DNA operators for TetR and MarR family transcription factors from Burkholderia xenovorans. Microbiology. 2012;158:571-82 pubmed publisher
    ..Our study provides a significant addition to this knowledge base and illustrates some general challenges of discovering operators on a large scale for prokaryotes...
  36. Cracan V, Padovani D, Banerjee R. IcmF is a fusion between the radical B12 enzyme isobutyryl-CoA mutase and its G-protein chaperone. J Biol Chem. 2010;285:655-66 pubmed publisher
  37. Erickson B, Mondello F. Nucleotide sequencing and transcriptional mapping of the genes encoding biphenyl dioxygenase, a multicomponent polychlorinated-biphenyl-degrading enzyme in Pseudomonas strain LB400. J Bacteriol. 1992;174:2903-12 pubmed
    ..Transcription initiating at the site furthest upstream is greatly increased when the LB400 cells are grown on biphenyl as the sole carbon source...
  38. Kumar P, Mohammadi M, Viger J, Barriault D, Gomez Gil L, Eltis L, et al. Structural insight into the expanded PCB-degrading abilities of a biphenyl dioxygenase obtained by directed evolution. J Mol Biol. 2011;405:531-47 pubmed publisher
    The biphenyl dioxygenase of Burkholderia xenovorans LB400 is a multicomponent Rieske-type oxygenase that catalyzes the dihydroxylation of biphenyl and many polychlorinated biphenyls (PCBs)...
  39. Baker P, Seah S. Rational design of stereoselectivity in the class II pyruvate aldolase BphI. J Am Chem Soc. 2012;134:507-13 pubmed publisher
    ..Thus, stereocomplementary class II pyruvate aldolases are now available to create chiral 4-hydroxy-2-oxoacid skeletons as synthons for organic reactions...
  40. Tocheva E, Fortin P, Eltis L, Murphy M. Structures of ternary complexes of BphK, a bacterial glutathione S-transferase that reductively dechlorinates polychlorinated biphenyl metabolites. J Biol Chem. 2006;281:30933-40 pubmed publisher
    ..BphK from Burkholderia xenovorans LB400 consumes two GSH molecules to reductively dehalogenate chlorinated 2-hydroxy-6-oxo-6-phenyl-2,4-..
  41. Taira K, Hirose J, Hayashida S, Furukawa K. Analysis of bph operon from the polychlorinated biphenyl-degrading strain of Pseudomonas pseudoalcaligenes KF707. J Biol Chem. 1992;267:4844-53 pubmed
    ..Thus the biphenyl degradation pathway and the responsible enzymes/genes are very similar to those of toluene degradation despite their discrete substrate specificity...
  42. Keller J, Baurick K, Rutt G, O Malley M, Sonafrank N, Reynolds R, et al. Pseudomonas cepacia 2,2-dialkylglycine decarboxylase. Sequence and expression in Escherichia coli of structural and repressor genes. J Biol Chem. 1990;265:5531-9 pubmed
    ..Expression is induced by S-isovaline, 2-methylalanine, and D-2-aminobutanoic acid, but not by glycine, D- or L-alanine, L-2-aminobutanoic acid, R-isovaline, or other alkyl amino acids...
  43. Han S, Eltis L, Timmis K, Muchmore S, Bolin J. Crystal structure of the biphenyl-cleaving extradiol dioxygenase from a PCB-degrading pseudomonad. Science. 1995;270:976-80 pubmed
    ..The iron atom has five ligands in square pyramidal geometry: one glutamate and two histidine side chains, and two water molecules...
  44. Toney M, Hohenester E, Keller J, Jansonius J. Structural and mechanistic analysis of two refined crystal structures of the pyridoxal phosphate-dependent enzyme dialkylglycine decarboxylase. J Mol Biol. 1995;245:151-79 pubmed publisher
  45. Hofer B, Backhaus S, Timmis K. The biphenyl/polychlorinated biphenyl-degradation locus (bph) of Pseudomonas sp. LB400 encodes four additional metabolic enzymes. Gene. 1994;144:9-16 pubmed
  46. Hofer B, Eltis L, Dowling D, Timmis K. Genetic analysis of a Pseudomonas locus encoding a pathway for biphenyl/polychlorinated biphenyl degradation. Gene. 1993;130:47-55 pubmed
    ..Six Bph have been detected and identified. Five of them could be obtained as the most abundant proteins when their genes were expressed in Escherichia coli...
  47. Malashkevich V, Strop P, Keller J, Jansonius J, Toney M. Crystal structures of dialkylglycine decarboxylase inhibitor complexes. J Mol Biol. 1999;294:193-200 pubmed publisher
    ..This difference explains the approximately 3000-fold greater potency of the l versus the d-isomer as a competitive inhibitor of dialkylglycine decarboxylase...
  48. Moulin L, Munive A, Dreyfus B, Boivin Masson C. Nodulation of legumes by members of the beta-subclass of Proteobacteria. Nature. 2001;411:948-50 pubmed publisher
    ..Here we report the identification of proteobacteria from the beta-subclass that nodulate legumes. This finding shows that the ability to establish a symbiosis with legumes is more widespread in bacteria than anticipated to date...
  49. Bains J, Boulanger M. Biochemical and structural characterization of the paralogous benzoate CoA ligases from Burkholderia xenovorans LB400: defining the entry point into the novel benzoate oxidation (box) pathway. J Mol Biol. 2007;373:965-77 pubmed publisher
    ..A novel benzoate oxidation (box) pathway has been identified recently in Burkholderia xenovorans LB400 (referred to simply as LB400) that is capable of assimilating benzoate and intimately tied to the ..
  50. Yeager C, Bottomley P, Arp D. Requirement of DNA repair mechanisms for survival of Burkholderia cepacia G4 upon degradation of trichloroethylene. Appl Environ Microbiol. 2001;67:5384-91 pubmed publisher
    ..cepacia G4 cells to survive following TCE transformation. A possible role for nucleotide excision repair and recombination repair activities in TCE-damaged cells is discussed...
  51. Coenye T, Mahenthiralingam E, Henry D, LiPuma J, Laevens S, Gillis M, et al. Burkholderia ambifaria sp. nov., a novel member of the Burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates. Int J Syst Evol Microbiol. 2001;51:1481-90 pubmed publisher
    ..cepacia complex would be ill-advised until more is known about their potential pathogenic mechanisms...
  52. Tomich M, Herfst C, Golden J, Mohr C. Role of flagella in host cell invasion by Burkholderia cepacia. Infect Immun. 2002;70:1799-806 pubmed
    ..cepacia. Our findings indicate that flagellum-mediated motility may facilitate penetration of host epithelial barriers by B. cepacia, contributing to establishment of infection and systemic spread of the organism...
  53. Rodrigues M, Valentin H, Berger P, Tran M, Asrar J, Gruys K, et al. Polyhydroxyalkanoate accumulation in Burkholderia sp.: a molecular approach to elucidate the genes involved in the formation of two homopolymers consisting of short-chain-length 3-hydroxyalkanoic acids. Appl Microbiol Biotechnol. 2000;53:453-60 pubmed
    ..The arrangement of the three genes is quite similar to the R. eutropha poly-beta-hydroxybutyrate biosynthesis operon...
  54. Vandamme P, Henry D, Coenye T, Nzula S, Vancanneyt M, LiPuma J, et al. Burkholderia anthina sp. nov. and Burkholderia pyrrocinia, two additional Burkholderia cepacia complex bacteria, may confound results of new molecular diagnostic tools. FEMS Immunol Med Microbiol. 2002;33:143-9 pubmed
    ..cepacia complex. This highlights another potential source for diagnostic problems with B. cepacia-like bacteria...
  55. Vermis K, Coenye T, LiPuma J, Mahenthiralingam E, Nelis H, Vandamme P. Proposal to accommodate Burkholderia cepacia genomovar VI as Burkholderia dolosa sp. nov. Int J Syst Evol Microbiol. 2004;54:689-91 pubmed publisher
    ..cepacia-selective medium PCAT. Both 16S rDNA and recA RFLP analysis revealed unique B. dolosa restriction patterns. In addition, new 16S rDNA- and recA-based PCR assays allowed its specific identification...
  56. Ramette A, LiPuma J, Tiedje J. Species abundance and diversity of Burkholderia cepacia complex in the environment. Appl Environ Microbiol. 2005;71:1193-201 pubmed publisher
    ..cepacia complex species and closely related strains of the same species may coexist at high population levels but also species composition and abundance may dramatically vary between individual plants...
  57. Payne G, Ramette A, Rose H, Weightman A, Jones T, Tiedje J, et al. Application of a recA gene-based identification approach to the maize rhizosphere reveals novel diversity in Burkholderia species. FEMS Microbiol Lett. 2006;259:126-32 pubmed publisher
  58. Ohtsubo Y, Goto H, Nagata Y, Kudo T, Tsuda M. Identification of a response regulator gene for catabolite control from a PCB-degrading beta-proteobacteria, Acidovorax sp. KKS102. Mol Microbiol. 2006;60:1563-75 pubmed publisher
    ..The genes highly similar to bphQ were found from several beta-proteobacteria, such as Burkholderia cenocepacia J2315, B. multivorans ATCC17616, B. xenovorans LB400 and Ralstonia solanacearum RS1085...
  59. Kerby R, Youn H, Roberts G. RcoM: a new single-component transcriptional regulator of CO metabolism in bacteria. J Bacteriol. 2008;190:3336-43 pubmed publisher
    ..The genetic linkage of rcoM with both aerobic (cox) and anaerobic (coo) CO oxidation systems suggests that in different organisms RcoM proteins may control either regulon type...