Burkholderia pseudomallei K96243


Alias: Burkholderia pseudomallei str. K96243, Burkholderia pseudomallei strain K96243

Top Publications

  1. DeShazer D, Brett P, Carlyon R, Woods D. Mutagenesis of Burkholderia pseudomallei with Tn5-OT182: isolation of motility mutants and molecular characterization of the flagellin structural gene. J Bacteriol. 1997;179:2116-25 pubmed
    ..pseudomallei infection. A phylogenetic analysis based on the flagellins from a variety of bacterial species supported the recent transfer of B. pseudomallei from the genus Pseudomonas to Burkholderia. ..
  2. Rainbow L, Hart C, Winstanley C. Distribution of type III secretion gene clusters in Burkholderia pseudomallei, B. thailandensis and B. mallei. J Med Microbiol. 2002;51:374-84 pubmed
    ..PCR assays indicated that TTS2 was also present in B. thailandensis. TTS1 and TTS2 are similar in gene organisation, but nucleotide sequences are sufficiently divergent to suggest that the two TTS systems may have different roles. ..
  3. Stevens M, Wood M, Taylor L, Monaghan P, Hawes P, Jones P, et al. An Inv/Mxi-Spa-like type III protein secretion system in Burkholderia pseudomallei modulates intracellular behaviour of the pathogen. Mol Microbiol. 2002;46:649-59 pubmed
    ..These findings indicate that the Bsa type III secretion system plays an essential role in modulating the intracellular behaviour of B. pseudomallei. ..
  4. Subsin B, Thomas M, Katzenmeier G, Shaw J, Tungpradabkul S, Kunakorn M. Role of the stationary growth phase sigma factor RpoS of Burkholderia pseudomallei in response to physiological stress conditions. J Bacteriol. 2003;185:7008-14 pubmed
    ..The mutant was shown to have an increased sensitivity to carbon starvation and oxidative stress. By using rpoS-lacZ fusions, transcription of rpoS was shown to be growth phase regulated, reaching a peak upon entry into stationary phase. ..
  5. Stevens M, Haque A, Atkins T, Hill J, Wood M, Easton A, et al. Attenuated virulence and protective efficacy of a Burkholderia pseudomallei bsa type III secretion mutant in murine models of melioidosis. Microbiology. 2004;150:2669-76 pubmed
    ..Mice inoculated with the B. pseudomallei bipD mutant were partially protected against subsequent challenge with wild-type B. pseudomallei. However, immunization of mice with purified BipD protein was not protective. ..
  6. Suparak S, Kespichayawattana W, Haque A, Easton A, Damnin S, Lertmemongkolchai G, et al. Multinucleated giant cell formation and apoptosis in infected host cells is mediated by Burkholderia pseudomallei type III secretion protein BipB. J Bacteriol. 2005;187:6556-60 pubmed
  7. Cullinane M, Gong L, Li X, Lazar Adler N, Tra T, Wolvetang E, et al. Stimulation of autophagy suppresses the intracellular survival of Burkholderia pseudomallei in mammalian cell lines. Autophagy. 2008;4:744-53 pubmed
    ..pseudomallei, but which the bacteria can actively evade. However, when autophagy is pharmacologically induced using rapamycin, bacteria are actively sequestered in autophagosomes, ultimately decreasing their survival. ..
  8. Tunpiboonsak S, Mongkolrob R, Kitudomsub K, Thanwatanaying P, Kiettipirodom W, Tungboontina Y, et al. Role of a Burkholderia pseudomallei polyphosphate kinase in an oxidative stress response, motilities, and biofilm formation. J Microbiol. 2010;48:63-70 pubmed publisher
    ..The B. pseudomallei flagellin (fliC) mutant and B. mallei, a nonmotile species, are shown to produce higher density biofilm formation than the ppk mutant, but less than wild type B. pseudomallei. ..
  9. Cruz Migoni A, Hautbergue G, Artymiuk P, Baker P, Bokori Brown M, Chang C, et al. A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A. Science. 2011;334:821-4 pubmed publisher
    ..BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase activity and inhibiting translation. We propose to name BPSL1549 Burkholderia lethal factor 1. ..

More Information

Publications105 found, 100 shown here

  1. Burtnick M, Brett P, Nair V, Warawa J, Woods D, Gherardini F. Burkholderia pseudomallei type III secretion system mutants exhibit delayed vacuolar escape phenotypes in RAW 264.7 murine macrophages. Infect Immun. 2008;76:2991-3000 pubmed publisher
    ..7 cells. Based upon these findings, it appears that B. pseudomallei may not require T3SS-1, -2, and -3 to facilitate survival, delayed vacuolar escape, and actin-based motility in activated RAW 264.7 macrophages. ..
  2. Tan K, Chen Y, Lim Y, Tan G, Liu Y, Lim Y, et al. Suppression of host innate immune response by Burkholderia pseudomallei through the virulence factor TssM. J Immunol. 2010;184:5160-71 pubmed publisher
    ..Abs to TssM can be detected in the sera of melioidosis patients, indicating that TssM is functionally expressed in vivo and thus could contribute to bacterial pathogenesis in human melioidosis...
  3. Mahenthiralingam E, Baldwin A, Vandamme P. Burkholderia cepacia complex infection in patients with cystic fibrosis. J Med Microbiol. 2002;51:533-8 pubmed publisher
    ..For microbiologists, hospital infection control officers, caregivers, and most of all the CF community, the changes in our understanding of the taxonomy, epidemiology and pathogenesis of the bacterium 'B. cepacia' are complex...
  4. Johnson S, Roversi P, Espina M, Olive A, Deane J, Birket S, et al. Self-chaperoning of the type III secretion system needle tip proteins IpaD and BipD. J Biol Chem. 2007;282:4035-44 pubmed publisher
    ..Crystal packing has allowed us to construct a model for the tip complex that is supported by mutations designed using the structure...
  5. MacLea K, Krieser R, Eastman A. A family history of deoxyribonuclease II: surprises from Trichinella spiralis and Burkholderia pseudomallei. Gene. 2003;305:1-12 pubmed
    ..We report an analysis of their sequences and implications for DNase II protein structure and evolution...
  6. Lassaux P, Peri C, Ferrer Navarro M, Gourlay L, Gori A, Conchillo Solé O, et al. A structure-based strategy for epitope discovery in Burkholderia pseudomallei OppA antigen. Structure. 2013;21:167-175 pubmed publisher
    ..Our results illustrate a structure-based epitope discovery process, whose application may expand our perspectives in the diagnostic and vaccine design fields. ..
  7. Zylstra G, Olsen R, Ballou D. Genetic organization and sequence of the Pseudomonas cepacia genes for the alpha and beta subunits of protocatechuate 3,4-dioxygenase. J Bacteriol. 1989;171:5915-21 pubmed
    ..The deduced amino acid sequence showed extensive homology with the previously determined amino acid sequence of protocatechuate 3,4-dioxygenase from another Pseudomonas species. ..
  8. van Waasbergen L, Kidambi S, Miller R. Construction of a recA mutant of Burkholderia (formerly Pseudomonas), cepacia. Appl Microbiol Biotechnol. 1998;49:59-65 pubmed
    ..cepacia and a recA mutant will be useful in bioengineering of this species. ..
  9. Burtnick M, Woods D. Isolation of polymyxin B-susceptible mutants of Burkholderia pseudomallei and molecular characterization of genetic loci involved in polymyxin B resistance. Antimicrob Agents Chemother. 1999;43:2648-56 pubmed
    ..pseudomallei 1026b...
  10. Singh A, Lai S, Nandi T, Chua H, Ooi W, Ong C, et al. Evolutionary analysis of Burkholderia pseudomallei identifies putative novel virulence genes, including a microbial regulator of host cell autophagy. J Bacteriol. 2013;195:5487-98 pubmed publisher
    ..pseudomallei intracellular growth. More generally, our study highlights the utility of applying evolutionary principles to microbial genomes to identify novel virulence genes. ..
  11. Chan C, Stead D, Coutts R. Development of a species-specific recA-based PCR test for Burkholderia fungorum. FEMS Microbiol Lett. 2003;224:133-8 pubmed
    ..Using a similar procedure we extended the recA-based PCR assay to identify B. sacchari and B. caledonica, two additional soil-inhabiting Burkholderia spp. ..
  12. Upadhyay A, Williams C, Gill A, Philippe D, Davis K, Taylor L, et al. Biophysical characterization of the catalytic domain of guanine nucleotide exchange factor BopE from Burkholderia pseudomallei. Biochim Biophys Acta. 2004;1698:111-9 pubmed
    ..These properties are likely to be advantageous for a secreted bacterial effector protein. ..
  13. Storms V, Van Den Vreken N, Coenye T, Mahenthiralingam E, LiPuma J, Gillis M, et al. Polyphasic characterisation of Burkholderia cepacia-like isolates leading to the emended description of Burkholderia pyrrocinia. Syst Appl Microbiol. 2004;27:517-26 pubmed
    ..cepacia complex species thus far comprising one single soil isolate only. An emended description of Burkholderia pyrrocinia is proposed. The taxonomic status of the remaining two isolates requires further analysis. ..
  14. Yates S, Edwards T, Bryan C, Stein A, Van Voorhis W, Myler P, et al. Structural basis of the substrate specificity of bifunctional isocitrate dehydrogenase kinase/phosphatase. Biochemistry. 2011;50:8103-6 pubmed publisher
    ..Kinetic analyses provided further confirmation that Bp-ICDH is a substrate for AceK. We conclude that the highly stringent AceK binding sites on ICDH are maintained only in Gram-negative bacteria...
  15. Gourlay L, Thomas R, Peri C, Conchillo Solé O, Ferrer Navarro M, Nithichanon A, et al. From crystal structure to in silico epitope discovery in the Burkholderia pseudomallei flagellar hook-associated protein FlgK. FEBS J. 2015;282:1319-33 pubmed publisher
    ..Taken together, our results point to FlgK(Bp) as a candidate for the design and production of epitope-containing subunits/domains as potential vaccine components. ..
  16. Niumsup P, Wuthiekanun V. Cloning of the class D beta-lactamase gene from Burkholderia pseudomallei and studies on its expression in ceftazidime-susceptible and -resistant strains. J Antimicrob Chemother. 2002;50:445-55 pubmed
    ..Reverse transcriptase PCR showed that transcription of the class D beta-lactamase gene is increased in ceftazidime-resistant mutants...
  17. Chan Y, Chua K. The Burkholderia pseudomallei BpeAB-OprB efflux pump: expression and impact on quorum sensing and virulence. J Bacteriol. 2005;187:4707-19 pubmed publisher
    ..pseudomallei virulence using inhibitors of the BpeAB-OprB efflux pump...
  18. Vanaporn M, Wand M, Michell S, Sarkar Tyson M, Ireland P, Goldman S, et al. Superoxide dismutase C is required for intracellular survival and virulence of Burkholderia pseudomallei. Microbiology. 2011;157:2392-400 pubmed publisher
    ..Our results indicate that SodC plays a key role in the virulence of B. pseudomallei, but that diabetics are not more susceptible to infection because of a reduced ability of PMNs to kill by superoxide...
  19. Nakazawa T, Kimoto M, Abe M. Cloning, sequencing, and transcriptional analysis of the recA gene of Pseudomonas cepacia. Gene. 1990;94:83-8 pubmed
    ..An SOS box characteristic to LexA-regulated promoters, along with the -10 and -35 consensus sequences, was found in the 5' upstream region of the P. cepacia recA gene. ..
  20. Charnock C. Structural studies of malate dehydrogenases (MDHs): MDHs in Brevundimonas species are the first reported MDHs in Proteobacteria which resemble lactate dehydrogenases in primary structure. J Bacteriol. 1997;179:4066-70 pubmed
    ..Additional evidence from subunit molecular weights, peptide mapping, and enzyme mobilities suggested that malate dehydrogenases from species of the genus Brevundimonas were structurally distinct from others in the study. ..
  21. Essex Lopresti A, Boddey J, Thomas R, Smith M, Hartley M, Atkins T, et al. A type IV pilin, PilA, Contributes To Adherence of Burkholderia pseudomallei and virulence in vivo. Infect Immun. 2005;73:1260-4 pubmed publisher
    The Burkholderia pseudomallei K96243 genome contains multiple type IV pilin-associated loci, including one encoding a putative pilus structural protein (pilA)...
  22. Deemagarn T, Wiseman B, Carpena X, Ivancich A, Fita I, Loewen P. Two alternative substrate paths for compound I formation and reduction in catalase-peroxidase KatG from Burkholderia pseudomallei. Proteins. 2007;66:219-28 pubmed
    ..It is proposed that binding of substrate H(2)O(2) to Asp141 and Arg108 controls H(2)O(2) access to the heme active site, thereby modulating the catalase reaction. ..
  23. Kang W, Vellasamy K, Chua E, Vadivelu J. Functional characterizations of effector protein BipC, a type III secretion system protein, in Burkholderia pseudomallei pathogenesis. J Infect Dis. 2015;211:827-34 pubmed publisher
    ..The results suggested that BipC possesses virulence determinants that play significant roles in host cell invasion and immune evasion. ..
  24. Gourlay L, Peano C, Deantonio C, Perletti L, Pietrelli A, Villa R, et al. Selecting soluble/foldable protein domains through single-gene or genomic ORF filtering: structure of the head domain of Burkholderia pseudomallei antigen BPSL2063. Acta Crystallogr D Biol Crystallogr. 2015;71:2227-35 pubmed publisher
    ..The results imply the further development of ORF-filtering methods as a tool in protein-based research to improve the selection and production of soluble proteins or domains for downstream applications such as X-ray crystallography. ..
  25. Sirijant N, Sermswan R, Wongratanacheewin S. Burkholderia pseudomallei resistance to antibiotics in biofilm-induced conditions is related to efflux pumps. J Med Microbiol. 2016;65:1296-1306 pubmed publisher
    ..pseudomallei in biofilm conditions. Inhibition of efflux pumps may lead to a decrease of resistance to CTZ and DOX in biofilm cells. ..
  26. Cruz Migoni A, Ruzheinikov S, Sedelnikova S, Obeng B, Chieng S, Mohamed R, et al. Cloning, purification and crystallographic analysis of a hypothetical protein, BPSL1549, from Burkholderia pseudomallei. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011;67:1623-6 pubmed publisher
    ..The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 37.1, b = 45.4, c = 111.9 Å and with a single polypeptide chain in the asymmetric unit. ..
  27. Ireland P, McMahon R, Marshall L, Halili M, Furlong E, Tay S, et al. Disarming Burkholderia pseudomallei: structural and functional characterization of a disulfide oxidoreductase (DsbA) required for virulence in vivo. Antioxid Redox Signal. 2014;20:606-17 pubmed publisher
    ..pseudomallei infection, which in combination with our structural characterization of BpsDsbA will facilitate the future development of rationally designed inhibitors against this drug-resistant organism. ..
  28. Pumirat P, Boonyuen U, Vanaporn M, Pinweha P, Tandhavanant S, Korbsrisate S, et al. The role of short-chain dehydrogenase/oxidoreductase, induced by salt stress, on host interaction of B. pseudomallei. BMC Microbiol. 2014;14:1 pubmed publisher
    ..Identifying the role of B. pseudomallei SDO provides a better understanding of the association between bacterial adaptation and pathogenesis in melioidosis. ..
  29. Reynolds C, Goudet A, Jenjaroen K, Sumonwiriya M, Rinchai D, Musson J, et al. T Cell Immunity to the Alkyl Hydroperoxide Reductase of Burkholderia pseudomallei: A Correlate of Disease Outcome in Acute Melioidosis. J Immunol. 2015;194:4814-24 pubmed publisher
    ..For an infection in which there has been debate about centrality of T cell immunity in defense, these observations support a role for T cell immunity to AhpC in disease protection. ..
  30. Moore R, DeShazer D, Reckseidler S, Weissman A, Woods D. Efflux-mediated aminoglycoside and macrolide resistance in Burkholderia pseudomallei. Antimicrob Agents Chemother. 1999;43:465-70 pubmed
    ..These results suggest that an efflux system is a major contributor to the inherent high-level aminoglycoside and macrolide resistance found in B. pseudomallei...
  31. Lowe C, Asghar A, Shalom G, Shaw J, Thomas M. The Burkholderia cepacia fur gene: co-localization with omlA and absence of regulation by iron. Microbiology. 2001;147:1303-14 pubmed
    ..Interestingly, the presence of the divergently transcribed omlA/smpA gene upstream of fur in some members of the gamma subclass of the Proteobacteria is retained in several genera within the beta taxon, including Burkholderia. ..
  32. Inose K, Fujikawa M, Yamazaki T, Kojima K, Sode K. Cloning and expression of the gene encoding catalytic subunit of thermostable glucose dehydrogenase from Burkholderia cepacia in Escherichia coli. Biochim Biophys Acta. 2003;1645:133-8 pubmed
    ..The deduced primary structure of gamma subunit showed about 30% identity to the small subunits of the SDH from G. oxydans and 2KGDHs from E. herbicola and P. citrea. ..
  33. Carpena X, Wiseman B, Deemagarn T, Herguedas B, Ivancich A, Singh R, et al. Roles for Arg426 and Trp111 in the modulation of NADH oxidase activity of the catalase-peroxidase KatG from Burkholderia pseudomallei inferred from pH-induced structural changes. Biochemistry. 2006;45:5171-9 pubmed publisher
  34. Roversi P, Johnson S, Field T, Deane J, Galyov E, Lea S. Expression, purification, crystallization and preliminary crystallographic analysis of BipD, a component of the Burkholderia pseudomallei type III secretion system. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006;62:861-4 pubmed
    ..7 A with radiation of wavelength lambda = 0.933 A. The Pt-derivative anomalous difference Patterson map revealed two self-peaks on the Harker sections. ..
  35. Jakopitsch C, Vlasits J, Wiseman B, Loewen P, Obinger C. Redox intermediates in the catalase cycle of catalase-peroxidases from Synechocystis PCC 6803, Burkholderia pseudomallei, and Mycobacterium tuberculosis. Biochemistry. 2007;46:1183-93 pubmed publisher
    ..Possible reaction intermediates involved in H2O2 dismutation by KatG are discussed...
  36. Wiseman B, Colin J, Smith A, Ivancich A, Loewen P. Mechanistic insight into the initiation step of the reaction of Burkholderia pseudomallei catalase-peroxidase with peroxyacetic acid. J Biol Inorg Chem. 2009;14:801-11 pubmed publisher
  37. Kim M, Shin D. A preliminary X-ray study of sedoheptulose-7-phosphate isomerase from Burkholderia pseudomallei. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009;65:1110-2 pubmed publisher
    ..3, b = 84.2, c = 142.3 angstrom. A full structural determination is under way in order to provide insights into the structure- function relationships of this protein. ..
  38. Austin C, Goodyear A, Bartek I, Stewart A, Sutherland M, Silva E, et al. A Burkholderia pseudomallei colony variant necessary for gastric colonization. MBio. 2015;6: pubmed publisher
    ..These findings demonstrate a role in infection for colony variation and provide a mechanism for chronic stomach colonization-a frequently overlooked niche in melioidosis. ..
  39. Nithichanon A, Rinchai D, Gori A, Lassaux P, Peri C, Conchillio Solé O, et al. Sequence- and Structure-Based Immunoreactive Epitope Discovery for Burkholderia pseudomallei Flagellin. PLoS Negl Trop Dis. 2015;9:e0003917 pubmed publisher
    ..The epitopes derived from this study may represent potential melioidosis vaccine components. ..
  40. Vanaporn M, Sarkar Tyson M, Kovács Simon A, Ireland P, Pumirat P, Korbsrisate S, et al. Trehalase plays a role in macrophage colonization and virulence of Burkholderia pseudomallei in insect and mammalian hosts. Virulence. 2017;8:30-40 pubmed publisher
    ..This is the first report that trehalase is important for bacterial virulence. ..
  41. Keith K, Oyston P, Crossett B, Fairweather N, Titball R, Walsh T, et al. Functional characterization of OXA-57, a class D beta-lactamase from Burkholderia pseudomallei. Antimicrob Agents Chemother. 2005;49:1639-41 pubmed publisher
    ..Comparative kinetic analyses of wild-type and mutant forms of B. pseudomallei OXA-57 are reported. Implications of these data for beta-lactam resistance and the proposed role of Ser-104 in beta-lactam hydrolysis are discussed...
  42. Nagata Y, Matsuda M, Komatsu H, Imura Y, Sawada H, Ohtsubo Y, et al. Organization and localization of the dnaA and dnaK gene regions on the multichromosomal genome of Burkholderia multivorans ATCC 17616. J Biosci Bioeng. 2005;99:603-10 pubmed
    ..Pulsed-field gel electrophoresis and Southern blot analyses indicated that both the dnaA and dnaK gene regions exist as single copies on the 3.4 Mb chromosome. ..
  43. Gourlay L, Peri C, Ferrer Navarro M, Conchillo Solé O, Gori A, Rinchai D, et al. Exploiting the Burkholderia pseudomallei acute phase antigen BPSL2765 for structure-based epitope discovery/design in structural vaccinology. Chem Biol. 2013;20:1147-56 pubmed publisher
    ..Our strategy represents a step in the development of immunodiagnostics, in the production of specific antibodies and in the optimization of antigens for vaccine development, starting from structural and physicochemical principles. ..
  44. Ahmad L, Hung T, Mat Akhir N, Mohamed R, Nathan S, Firdaus Raih M. Characterization of Burkholderia pseudomallei protein BPSL1375 validates the Putative hemolytic activity of the COG3176 N-Acyltransferase family. BMC Microbiol. 2015;15:270 pubmed publisher
  45. Loprasert S, Sallabhan R, Whangsuk W, Mongkolsuk S. Characterization and mutagenesis of fur gene from Burkholderia pseudomallei. Gene. 2000;254:129-37 pubmed
    ..Interestingly, Fur was found to play roles as a positive regulator of FeSOD and peroxidase. The mutant showed a decreased activity of FeSOD and peroxidase, which could be important in its pathogenicity and survival in macrophages...
  46. Jones A, DeShazer D, Woods D. Identification and characterization of a two-component regulatory system involved in invasion of eukaryotic cells and heavy-metal resistance in Burkholderia pseudomallei. Infect Immun. 1997;65:4972-7 pubmed
    ..pseudomallei infection...
  47. DeShazer D, Brett P, Woods D. The type II O-antigenic polysaccharide moiety of Burkholderia pseudomallei lipopolysaccharide is required for serum resistance and virulence. Mol Microbiol. 1998;30:1081-100 pubmed
    ..The proteins encoded by these genes were similar to proteins involved in bacterial polysaccharide biosynthesis. The results presented here demonstrate that type II O-PS is essential for B. pseudomallei serum resistance and virulence...
  48. DeShazer D, Brett P, Burtnick M, Woods D. Molecular characterization of genetic loci required for secretion of exoproducts in Burkholderia pseudomallei. J Bacteriol. 1999;181:4661-4 pubmed
    ..In this study, we isolated and characterized 29 transposon mutants unable to secrete protease, lipase, and PLC...
  49. Lee M, Liu Y. Sequencing and characterization of a novel serine metalloprotease from Burkholderia pseudomallei. FEMS Microbiol Lett. 2000;192:67-72 pubmed
    ..The predicted amino acid sequence shares homology with the subtilisin family of serine proteases...
  50. Cheung T, Ho P, Woo P, Yuen K, Chau P. Cloning and expression of class A beta-lactamase gene blaA(BPS) in Burkholderia pseudomallei. Antimicrob Agents Chemother. 2002;46:1132-5 pubmed
    ..7. Sequence analysis of BPS-1 revealed conserved motifs typical of class A beta-lactamases and a relationship to the PenA (in B. cepacia) and BlaI (in Yersinia enterocolitica) lineages...
  51. Kuo A, Gulbis J, Antcliff J, Rahman T, Lowe E, Zimmer J, et al. Crystal structure of the potassium channel KirBac1.1 in the closed state. Science. 2003;300:1922-6 pubmed publisher
    ..This further suggests that initiation of gating by membrane or intracellular signals represents different entry points to a common mechanistic pathway...
  52. Donald L, Krokhin O, Duckworth H, Wiseman B, Deemagarn T, Singh R, et al. Characterization of the catalase-peroxidase KatG from Burkholderia pseudomallei by mass spectrometry. J Biol Chem. 2003;278:35687-92 pubmed publisher
    ..Tryptic peptide analysis and N-terminal sequencing revealed that the N-terminal residue of BpKatG is Ser22...
  53. Farmer K, Thomas M. Isolation and characterization of Burkholderia cenocepacia mutants deficient in pyochelin production: pyochelin biosynthesis is sensitive to sulfur availability. J Bacteriol. 2004;186:270-7 pubmed
    ..These observations suggest that, under conditions of sulfur depletion, cysteine cannot be spared for production of pyochelin even under iron starvation conditions...
  54. Chan Y, Tan T, Ong Y, Chua K. BpeAB-OprB, a multidrug efflux pump in Burkholderia pseudomallei. Antimicrob Agents Chemother. 2004;48:1128-35 pubmed
    ..The broad-spectrum efflux pump inhibitor MC-207,110 did not potentiate the effectiveness of the antimicrobials erythromycin and streptomycin in B. pseudomallei...
  55. Song Y, Xie C, Ong Y, Gan Y, Chua K. The BpsIR quorum-sensing system of Burkholderia pseudomallei. J Bacteriol. 2005;187:785-90 pubmed publisher
    ..Cell density-dependent expression of bpsI and bpsR, the positive regulation of bpsIR expression by BpsR, and the synthesis of N-octanoyl-homoserine lactone (C8HSL) by BpsI are described in this report...
  56. Carpena X, Wiseman B, Deemagarn T, Singh R, Switala J, Ivancich A, et al. A molecular switch and electronic circuit modulate catalase activity in catalase-peroxidases. EMBO Rep. 2005;6:1156-62 pubmed publisher
  57. Diggle S, Lumjiaktase P, Dipilato F, Winzer K, Kunakorn M, Barrett D, et al. Functional genetic analysis reveals a 2-Alkyl-4-quinolone signaling system in the human pathogen Burkholderia pseudomallei and related bacteria. Chem Biol. 2006;13:701-10 pubmed publisher
    ..These data reveal a role for AHQs in bacterial cell-to-cell communication beyond that seen in P. aeruginosa...
  58. Erskine P, Knight M, Ruaux A, Mikolajek H, Wong Fat Sang N, Withers J, et al. High resolution structure of BipD: an invasion protein associated with the type III secretion system of Burkholderia pseudomallei. J Mol Biol. 2006;363:125-36 pubmed publisher
    ..Comparison of the structure of BipD with immunological and other data for IpaD indicates that the C-terminal alpha-helix is also involved in contacts with other proteins that form the translocon...
  59. Haque A, Chu K, Easton A, Stevens M, Galyov E, Atkins T, et al. A live experimental vaccine against Burkholderia pseudomallei elicits CD4+ T cell-mediated immunity, priming T cells specific for 2 type III secretion system proteins. J Infect Dis. 2006;194:1241-8 pubmed publisher
    ..CD4+ and CD8+ cell depletion studies demonstrated that CD4+ cells, but not CD8+ cells, mediated this protection in vivo. Thus, CD4+ T cells can mediate vaccine-induced immunity to experimental melioidosis...
  60. Upadhyay A, Wu H, Williams C, Field T, Galyov E, van den Elsen J, et al. The guanine-nucleotide-exchange factor BopE from Burkholderia pseudomallei adopts a compact version of the Salmonella SopE/SopE2 fold and undergoes a closed-to-open conformational change upon interaction with Cdc42. Biochem J. 2008;411:485-93 pubmed publisher
    ..Requirement for closed-to-open conformational change explains the 10-40-fold lower k(cat) of BopE compared with SopE and SopE2...
  61. Vanaporn M, Vattanaviboon P, Thongboonkerd V, Korbsrisate S. The rpoE operon regulates heat stress response in Burkholderia pseudomallei. FEMS Microbiol Lett. 2008;284:191-6 pubmed publisher
    ..e. GroEL and HtpG) were also absent in the rpoE operon mutant. Altogether, our data suggest that the rpoE operon regulates B. pseudomallei heat stress response through the function of rpoH...
  62. M ller C, Conejero L, Spink N, Wand M, Bancroft G, Titball R. Role of RelA and SpoT in Burkholderia pseudomallei virulence and immunity. Infect Immun. 2012;80:3247-55 pubmed publisher
    ..pseudomallei, and the ?relA ?spoT mutant may be a promising live-attenuated vaccine candidate...
  63. Winstanley C, Hales B, Corkill J, Gallagher M, Hart C. Flagellin gene variation between clinical and environmental isolates of Burkholderia pseudomallei contrasts with the invariance among clinical isolates. J Med Microbiol. 1998;47:689-94 pubmed publisher
    ..pseudomallei strains...
  64. Winstanley C, Hales B, Hart C. Evidence for the presence in Burkholderia pseudomallei of a type III secretion system-associated gene cluster. J Med Microbiol. 1999;48:649-56 pubmed publisher
    ..solanacearum proteins. The presence of type III secretion-associated genes in B. pseudomallei pathogens suggests a possible role for type III secretion systems in the pathogenicity of this organism...
  65. Reckseidler S, DeShazer D, Sokol P, Woods D. Detection of bacterial virulence genes by subtractive hybridization: identification of capsular polysaccharide of Burkholderia pseudomallei as a major virulence determinant. Infect Immun. 2001;69:34-44 pubmed publisher
    ..Thus, the combination of PCR-based subtractive hybridization, insertional inactivation, and animal virulence studies has facilitated the identification of an important virulence determinant in B. pseudomallei...
  66. Burtnick M, Bolton A, Brett P, Watanabe D, Woods D. Identification of the acid phosphatase (acpA) gene homologues in pathogenic and non-pathogenic Burkholderia spp. facilitates TnphoA mutagenesis. Microbiology. 2001;147:111-20 pubmed publisher
    ..Two such systems have been successfully utilized in Burkholderia spp. for the identification of several genes encoding exported proteins...
  67. Loprasert S, Sallabhan R, Whangsuk W, Mongkolsuk S. The Burkholderia pseudomallei oxyR gene: expression analysis and mutant characterization. Gene. 2002;296:161-9 pubmed
    ..The altered phenotypes of oxyR deficient mutants were complemented when a copy of oxyR was transposed into the mutant chromosomes on the mini-Tn5 transposon...
  68. Stevens M, Friebel A, Taylor L, Wood M, Brown P, Hardt W, et al. A Burkholderia pseudomallei type III secreted protein, BopE, facilitates bacterial invasion of epithelial cells and exhibits guanine nucleotide exchange factor activity. J Bacteriol. 2003;185:4992-6 pubmed
    ..Consistent with this notion, BopE expressed in eukaryotic cells induced rearrangements in the subcortical actin cytoskeleton, and purified BopE exhibited guanine nucleotide exchange factor activity for Cdc42 and Rac1 in vitro...
  69. Corbett C, Burtnick M, Kooi C, Woods D, Sokol P. An extracellular zinc metalloprotease gene of Burkholderia cepacia. Microbiology. 2003;149:2263-71 pubmed publisher
    ..cepacia strain Pc715j and a Pc715j zmpA mutant. The results indicate that this extracellular zinc metalloprotease may play a greater role in virulence in some strains of B. cepacia...
  70. Druar C, Yu F, Barnes J, Okinaka R, Chantratita N, Beg S, et al. Evaluating Burkholderia pseudomallei Bip proteins as vaccines and Bip antibodies as detection agents. FEMS Immunol Med Microbiol. 2008;52:78-87 pubmed publisher
    ..However, these ELISAs had low diagnostic accuracy in endemic regions, possibly due to previous patient exposure to B. pseudomallei...
  71. Holmes M, Liwak U, Pricop I, Wang X, Tomavo S, Ananvoranich S. Silencing of tachyzoite enolase 2 alters nuclear targeting of bradyzoite enolase 1 in Toxoplasma gondii. Microbes Infect. 2010;12:19-27 pubmed publisher
    ..While there was no change in the growth rate of both tachyzoites and bradyzoites, a subtle phenotypic change was observed in the localization of the ENO1 gene product in the bradyzoite stage...
  72. Gong L, Cullinane M, Treerat P, Ramm G, Prescott M, Adler B, et al. The Burkholderia pseudomallei type III secretion system and BopA are required for evasion of LC3-associated phagocytosis. PLoS ONE. 2011;6:e17852 pubmed publisher
    ..We conclude that LC3 recruitment to phagosomes stimulates killing of B. pseudomallei trapped in phagosomes. Furthermore, BopA plays an important role in efficient escape of B. pseudomallei from phagosomes...
  73. Norville I, Breitbach K, Eske Pogodda K, Harmer N, Sarkar Tyson M, Titball R, et al. A novel FK-506-binding-like protein that lacks peptidyl-prolyl isomerase activity is involved in intracellular infection and in vivo virulence of Burkholderia pseudomallei. Microbiology. 2011;157:2629-38 pubmed publisher
    ..To our knowledge this B. pseudomallei FKBP represents the first example of this protein family which lacks PPIase activity but is important in intracellular infection of a bacterial pathogen...
  74. Bugrysheva J, Sue D, Gee J, Elrod M, Hoffmaster A, Randall L, et al. Antibiotic Resistance Markers in Burkholderia pseudomallei Strain Bp1651 Identified by Genome Sequence Analysis. Antimicrob Agents Chemother. 2017;61: pubmed publisher
    ..These findings expand the role of penA to include resistance to carbapenems and may assist in the development of molecular diagnostics that predict antimicrobial resistance and provide guidance for treatment of melioidosis. ..
  75. Yeager C, Bottomley P, Arp D. Requirement of DNA repair mechanisms for survival of Burkholderia cepacia G4 upon degradation of trichloroethylene. Appl Environ Microbiol. 2001;67:5384-91 pubmed publisher
    ..cepacia G4 cells to survive following TCE transformation. A possible role for nucleotide excision repair and recombination repair activities in TCE-damaged cells is discussed...
  76. Vandamme P, Henry D, Coenye T, Nzula S, Vancanneyt M, LiPuma J, et al. Burkholderia anthina sp. nov. and Burkholderia pyrrocinia, two additional Burkholderia cepacia complex bacteria, may confound results of new molecular diagnostic tools. FEMS Immunol Med Microbiol. 2002;33:143-9 pubmed
    ..cepacia complex. This highlights another potential source for diagnostic problems with B. cepacia-like bacteria...
  77. Siritapetawee J, Prinz H, Samosornsuk W, Ashley R, Suginta W. Functional reconstitution, gene isolation and topology modelling of porins from Burkholderia pseudomallei and Burkholderia thailandensis. Biochem J. 2004;377:579-87 pubmed publisher
    ..Topology prediction and molecular modelling suggest that this newly-isolated and cloned porin is a 16-stranded beta-barrel and the external loops of the protein could be important determinants of the immune response to infection...
  78. Ramette A, LiPuma J, Tiedje J. Species abundance and diversity of Burkholderia cepacia complex in the environment. Appl Environ Microbiol. 2005;71:1193-201 pubmed publisher
    ..cepacia complex species and closely related strains of the same species may coexist at high population levels but also species composition and abundance may dramatically vary between individual plants...
  79. Goytia M, Chamond N, Cosson A, Coatnoan N, Hermant D, Berneman A, et al. Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens. PLoS ONE. 2007;2:e885 pubmed publisher
  80. Kang Y, Carlson R, Tharpe W, Schell M. Characterization of genes involved in biosynthesis of a novel antibiotic from Burkholderia cepacia BC11 and their role in biological control of Rhizoctonia solani. Appl Environ Microbiol. 1998;64:3939-47 pubmed
    ..DNA sequence analysis suggested that this region encodes proteins directly involved in the production of a nonribosomally synthesized lipopeptide...
  81. Tungpradabkul S, Wajanarogana S, Tunpiboonsak S, Panyim S. PCR-RFLP analysis of the flagellin sequences for identification of Burkholderia pseudomallei and Burkholderia cepacia from clinical isolates. Mol Cell Probes. 1999;13:99-105 pubmed publisher
    ..cepacia, to enable flagellin-type identification on the basis of the pattern of restriction fragments. In addition, the flagellin gene should be of considerable use as a genetic marker for clinical identification of these organisms...
  82. Rodrigues M, Valentin H, Berger P, Tran M, Asrar J, Gruys K, et al. Polyhydroxyalkanoate accumulation in Burkholderia sp.: a molecular approach to elucidate the genes involved in the formation of two homopolymers consisting of short-chain-length 3-hydroxyalkanoic acids. Appl Microbiol Biotechnol. 2000;53:453-60 pubmed
    ..The arrangement of the three genes is quite similar to the R. eutropha poly-beta-hydroxybutyrate biosynthesis operon...
  83. Woo P, Leung P, Tsoi H, Yuen K. Cloning and characterisation of malE in Burkholderia pseudomallei. J Med Microbiol. 2001;50:330-8 pubmed
    ..Further studies on serodiagnosis of melioidosis with recombinant MBP should be performed...
  84. Coenye T, Mahenthiralingam E, Henry D, LiPuma J, Laevens S, Gillis M, et al. Burkholderia ambifaria sp. nov., a novel member of the Burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates. Int J Syst Evol Microbiol. 2001;51:1481-90 pubmed publisher
    ..cepacia complex would be ill-advised until more is known about their potential pathogenic mechanisms...
  85. Tomich M, Herfst C, Golden J, Mohr C. Role of flagella in host cell invasion by Burkholderia cepacia. Infect Immun. 2002;70:1799-806 pubmed
    ..cepacia. Our findings indicate that flagellum-mediated motility may facilitate penetration of host epithelial barriers by B. cepacia, contributing to establishment of infection and systemic spread of the organism...
  86. Valvano M, Messner P, Kosma P. Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides. Microbiology. 2002;148:1979-89 pubmed publisher
  87. Ho P, Cheung T, Yam W, Yuen K. Characterization of a laboratory-generated variant of BPS beta-lactamase from Burkholderia pseudomallei that hydrolyses ceftazidime. J Antimicrob Chemother. 2002;50:723-6 pubmed
    ..coli conferred resistance to ceftazidime (MIC >or= 32 mg/L). The amino acid sequence of BPS-1m differed from that of BPS-1 by a Pro-to-Ser change at position 167 in the omega loop...
  88. Aguilar C, Bertani I, Venturi V. Quorum-sensing system and stationary-phase sigma factor (rpoS) of the onion pathogen Burkholderia cepacia genomovar I type strain, ATCC 25416. Appl Environ Microbiol. 2003;69:1739-47 pubmed
    ..cepacia has a negative effect on rpoS expression as determined by using an rpoS-lacZ transcriptional fusion; on the other hand, rpoS-null mutants displayed no difference in the accumulation of HSL signal molecules...
  89. Tribuddharat C, Moore R, Baker P, Woods D. Burkholderia pseudomallei class a beta-lactamase mutations that confer selective resistance against ceftazidime or clavulanic acid inhibition. Antimicrob Agents Chemother. 2003;47:2082-7 pubmed
    ..pseudomallei penA gene revealed a putative regulator gene located downstream of penA. We have cloned and sequenced the penA gene from B. mallei and found it to be identical to penA from B. pseudomallei...
  90. Vermis K, Coenye T, LiPuma J, Mahenthiralingam E, Nelis H, Vandamme P. Proposal to accommodate Burkholderia cepacia genomovar VI as Burkholderia dolosa sp. nov. Int J Syst Evol Microbiol. 2004;54:689-91 pubmed publisher
    ..cepacia-selective medium PCAT. Both 16S rDNA and recA RFLP analysis revealed unique B. dolosa restriction patterns. In addition, new 16S rDNA- and recA-based PCR assays allowed its specific identification...
  91. Zhang L, Wang Y, Picking W, Picking W, De Guzman R. Solution structure of monomeric BsaL, the type III secretion needle protein of Burkholderia pseudomallei. J Mol Biol. 2006;359:322-30 pubmed publisher
  92. Payne G, Ramette A, Rose H, Weightman A, Jones T, Tiedje J, et al. Application of a recA gene-based identification approach to the maize rhizosphere reveals novel diversity in Burkholderia species. FEMS Microbiol Lett. 2006;259:126-32 pubmed publisher