Burkholderia mallei ATCC 23344


Alias: Burkholderia mallei str. ATCC 23344

Top Publications

  1. Yeager C, Bottomley P, Arp D. Requirement of DNA repair mechanisms for survival of Burkholderia cepacia G4 upon degradation of trichloroethylene. Appl Environ Microbiol. 2001;67:5384-91 pubmed publisher
    ..cepacia G4 cells to survive following TCE transformation. A possible role for nucleotide excision repair and recombination repair activities in TCE-damaged cells is discussed...
  2. Stevens J, Ulrich R, Taylor L, Wood M, DeShazer D, Stevens M, et al. Actin-binding proteins from Burkholderia mallei and Burkholderia thailandensis can functionally compensate for the actin-based motility defect of a Burkholderia pseudomallei bimA mutant. J Bacteriol. 2005;187:7857-62 pubmed
    ..pseudomallei bimA mutant. While the BimA homologs differ in their amino-terminal sequence, they interact directly with actin in vitro and vary in their ability to bind Arp3. ..
  3. Burtnick M, Bolton A, Brett P, Watanabe D, Woods D. Identification of the acid phosphatase (acpA) gene homologues in pathogenic and non-pathogenic Burkholderia spp. facilitates TnphoA mutagenesis. Microbiology. 2001;147:111-20 pubmed publisher
    ..Two such systems have been successfully utilized in Burkholderia spp. for the identification of several genes encoding exported proteins...
  4. Zylstra G, Olsen R, Ballou D. Genetic organization and sequence of the Pseudomonas cepacia genes for the alpha and beta subunits of protocatechuate 3,4-dioxygenase. J Bacteriol. 1989;171:5915-21 pubmed
    ..The deduced amino acid sequence showed extensive homology with the previously determined amino acid sequence of protocatechuate 3,4-dioxygenase from another Pseudomonas species. ..
  5. Nakazawa T, Kimoto M, Abe M. Cloning, sequencing, and transcriptional analysis of the recA gene of Pseudomonas cepacia. Gene. 1990;94:83-8 pubmed
    ..An SOS box characteristic to LexA-regulated promoters, along with the -10 and -35 consensus sequences, was found in the 5' upstream region of the P. cepacia recA gene. ..
  6. Lafontaine E, Balder R, Michel F, Hogan R. Characterization of an autotransporter adhesin protein shared by Burkholderia mallei and Burkholderia pseudomallei. BMC Microbiol. 2014;14:92 pubmed publisher
    ..mallei and B. pseudomallei which mediates adherence to respiratory epithelial cells and is expressed in vivo during the course of aerosol infection. ..
  7. Woo P, Lau S, Woo G, Fung A, Ngan A, Hui W, et al. Seronegative bacteremic melioidosis caused by Burkholderia pseudomallei with ambiguous biochemical profile: clinical importance of accurate identification by 16S rRNA gene and groEL gene sequencing. J Clin Microbiol. 2003;41:3973-7 pubmed
    ..Sequencing of the groEL gene showed that the isolate was B. pseudomallei. Proper identification of the bacterium in this study is crucial, since there would be a radical difference in the duration of antimicrobial therapy. ..
  8. Montebello A, Brecht R, Turner R, Ghali M, Pu X, Nagarajan R. Acyl-ACP substrate recognition in Burkholderia mallei BmaI1 acyl-homoserine lactone synthase. Biochemistry. 2014;53:6231-42 pubmed publisher
    ..These results offer insights into the molecular basis of substrate recognition for the BmaI1 enzyme. ..
  9. Storms V, Van Den Vreken N, Coenye T, Mahenthiralingam E, LiPuma J, Gillis M, et al. Polyphasic characterisation of Burkholderia cepacia-like isolates leading to the emended description of Burkholderia pyrrocinia. Syst Appl Microbiol. 2004;27:517-26 pubmed
    ..cepacia complex species thus far comprising one single soil isolate only. An emended description of Burkholderia pyrrocinia is proposed. The taxonomic status of the remaining two isolates requires further analysis. ..

More Information


  1. Detsika M, Corkill J, Magalhaes M, Glendinning K, Hart C, Winstanley C. Molecular typing of, and distribution of genetic markers among, Burkholderia cepacia complex isolates from Brazil. J Clin Microbiol. 2003;41:4148-53 pubmed
    ..Nine genomovar IIIA isolates from different non-CF patients and clinical sources had identical genotypes, indicating the presence of a common clone. ..
  2. Chan C, Stead D, Coutts R. Development of a species-specific recA-based PCR test for Burkholderia fungorum. FEMS Microbiol Lett. 2003;224:133-8 pubmed
    ..Using a similar procedure we extended the recA-based PCR assay to identify B. sacchari and B. caledonica, two additional soil-inhabiting Burkholderia spp. ..
  3. Inose K, Fujikawa M, Yamazaki T, Kojima K, Sode K. Cloning and expression of the gene encoding catalytic subunit of thermostable glucose dehydrogenase from Burkholderia cepacia in Escherichia coli. Biochim Biophys Acta. 2003;1645:133-8 pubmed
    ..The deduced primary structure of gamma subunit showed about 30% identity to the small subunits of the SDH from G. oxydans and 2KGDHs from E. herbicola and P. citrea. ..
  4. van Waasbergen L, Kidambi S, Miller R. Construction of a recA mutant of Burkholderia (formerly Pseudomonas), cepacia. Appl Microbiol Biotechnol. 1998;49:59-65 pubmed
    ..cepacia and a recA mutant will be useful in bioengineering of this species. ..
  5. Nagata Y, Matsuda M, Komatsu H, Imura Y, Sawada H, Ohtsubo Y, et al. Organization and localization of the dnaA and dnaK gene regions on the multichromosomal genome of Burkholderia multivorans ATCC 17616. J Biosci Bioeng. 2005;99:603-10 pubmed
    ..Pulsed-field gel electrophoresis and Southern blot analyses indicated that both the dnaA and dnaK gene regions exist as single copies on the 3.4 Mb chromosome. ..
  6. Zhang L, Wang Y, Picking W, Picking W, De Guzman R. Solution structure of monomeric BsaL, the type III secretion needle protein of Burkholderia pseudomallei. J Mol Biol. 2006;359:322-30 pubmed publisher
  7. Payne G, Ramette A, Rose H, Weightman A, Jones T, Tiedje J, et al. Application of a recA gene-based identification approach to the maize rhizosphere reveals novel diversity in Burkholderia species. FEMS Microbiol Lett. 2006;259:126-32 pubmed publisher
  8. Ramette A, LiPuma J, Tiedje J. Species abundance and diversity of Burkholderia cepacia complex in the environment. Appl Environ Microbiol. 2005;71:1193-201 pubmed publisher
    ..cepacia complex species and closely related strains of the same species may coexist at high population levels but also species composition and abundance may dramatically vary between individual plants...
  9. Tomich M, Herfst C, Golden J, Mohr C. Role of flagella in host cell invasion by Burkholderia cepacia. Infect Immun. 2002;70:1799-806 pubmed
    ..cepacia. Our findings indicate that flagellum-mediated motility may facilitate penetration of host epithelial barriers by B. cepacia, contributing to establishment of infection and systemic spread of the organism...
  10. Vermis K, Coenye T, LiPuma J, Mahenthiralingam E, Nelis H, Vandamme P. Proposal to accommodate Burkholderia cepacia genomovar VI as Burkholderia dolosa sp. nov. Int J Syst Evol Microbiol. 2004;54:689-91 pubmed publisher
    ..cepacia-selective medium PCAT. Both 16S rDNA and recA RFLP analysis revealed unique B. dolosa restriction patterns. In addition, new 16S rDNA- and recA-based PCR assays allowed its specific identification...
  11. Schell M, Ulrich R, Ribot W, Brueggemann E, Hines H, Chen D, et al. Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 2007;64:1466-85 pubmed publisher
    ..Purified His-tagged BMAA0742 was recognized by glanders antiserum from a horse, a human and mice, indicating that this Hcp-family protein is produced in vivo during infection...
  12. Tribuddharat C, Moore R, Baker P, Woods D. Burkholderia pseudomallei class a beta-lactamase mutations that confer selective resistance against ceftazidime or clavulanic acid inhibition. Antimicrob Agents Chemother. 2003;47:2082-7 pubmed
    ..pseudomallei penA gene revealed a putative regulator gene located downstream of penA. We have cloned and sequenced the penA gene from B. mallei and found it to be identical to penA from B. pseudomallei...
  13. Aguilar C, Bertani I, Venturi V. Quorum-sensing system and stationary-phase sigma factor (rpoS) of the onion pathogen Burkholderia cepacia genomovar I type strain, ATCC 25416. Appl Environ Microbiol. 2003;69:1739-47 pubmed
    ..cepacia has a negative effect on rpoS expression as determined by using an rpoS-lacZ transcriptional fusion; on the other hand, rpoS-null mutants displayed no difference in the accumulation of HSL signal molecules...
  14. Burtnick M, Brett P, Woods D. Molecular and physical characterization of Burkholderia mallei O antigens. J Bacteriol. 2002;184:849-52 pubmed
    ..pseudomallei O polysaccharide (O-PS). In this study, we identified the O-PS biosynthetic gene cluster from B. mallei ATCC 23344 and subsequently characterized the molecular structure of the O-PS produced by this organism...
  15. Bandara A, DeShazer D, Inzana T, Sriranganathan N, Schurig G, Boyle S. A disruption of ctpA encoding carboxy-terminal protease attenuates Burkholderia mallei and induces partial protection in CD1 mice. Microb Pathog. 2008;45:207-16 pubmed publisher
    ..mallei ATCC 23344. These findings suggest that CtpA regulates in vitro growth, cell morphology and intracellular survival of B. mallei, and a ctpA mutant protects CD1 mice against glanders...
  16. Kang Y, Carlson R, Tharpe W, Schell M. Characterization of genes involved in biosynthesis of a novel antibiotic from Burkholderia cepacia BC11 and their role in biological control of Rhizoctonia solani. Appl Environ Microbiol. 1998;64:3939-47 pubmed
    ..DNA sequence analysis suggested that this region encodes proteins directly involved in the production of a nonribosomally synthesized lipopeptide...
  17. Wang Y, Ouellette A, Egan C, Rathinavelan T, Im W, De Guzman R. Differences in the electrostatic surfaces of the type III secretion needle proteins PrgI, BsaL, and MxiH. J Mol Biol. 2007;371:1304-14 pubmed publisher
    ..typhimurium and S. flexneri...
  18. Ohtsubo Y, Goto H, Nagata Y, Kudo T, Tsuda M. Identification of a response regulator gene for catabolite control from a PCB-degrading beta-proteobacteria, Acidovorax sp. KKS102. Mol Microbiol. 2006;60:1563-75 pubmed publisher
    ..The genes highly similar to bphQ were found from several beta-proteobacteria, such as Burkholderia cenocepacia J2315, B. multivorans ATCC17616, B. xenovorans LB400 and Ralstonia solanacearum RS1085...
  19. Zajdowicz S, Jones Carson J, Vazquez Torres A, Jobling M, Gill R, Holmes R. Alanine racemase mutants of Burkholderia pseudomallei and Burkholderia mallei and use of alanine racemase as a non-antibiotic-based selectable marker. PLoS ONE. 2011;6:e21523 pubmed publisher
    ..pseudomallei K96243. In addition, the alanine racemase deficient mutant of B. pseudomallei K96243 exhibited attenuation versus its isogenic parental strain with respect to growth and survival in murine peritoneal macrophages...
  20. Rodrigues M, Valentin H, Berger P, Tran M, Asrar J, Gruys K, et al. Polyhydroxyalkanoate accumulation in Burkholderia sp.: a molecular approach to elucidate the genes involved in the formation of two homopolymers consisting of short-chain-length 3-hydroxyalkanoic acids. Appl Microbiol Biotechnol. 2000;53:453-60 pubmed
    ..The arrangement of the three genes is quite similar to the R. eutropha poly-beta-hydroxybutyrate biosynthesis operon...
  21. Tungpradabkul S, Wajanarogana S, Tunpiboonsak S, Panyim S. PCR-RFLP analysis of the flagellin sequences for identification of Burkholderia pseudomallei and Burkholderia cepacia from clinical isolates. Mol Cell Probes. 1999;13:99-105 pubmed publisher
    ..cepacia, to enable flagellin-type identification on the basis of the pattern of restriction fragments. In addition, the flagellin gene should be of considerable use as a genetic marker for clinical identification of these organisms...
  22. Siritapetawee J, Prinz H, Samosornsuk W, Ashley R, Suginta W. Functional reconstitution, gene isolation and topology modelling of porins from Burkholderia pseudomallei and Burkholderia thailandensis. Biochem J. 2004;377:579-87 pubmed publisher
    ..Topology prediction and molecular modelling suggest that this newly-isolated and cloned porin is a 16-stranded beta-barrel and the external loops of the protein could be important determinants of the immune response to infection...
  23. Coenye T, Mahenthiralingam E, Henry D, LiPuma J, Laevens S, Gillis M, et al. Burkholderia ambifaria sp. nov., a novel member of the Burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates. Int J Syst Evol Microbiol. 2001;51:1481-90 pubmed publisher
    ..cepacia complex would be ill-advised until more is known about their potential pathogenic mechanisms...
  24. Ho P, Cheung T, Yam W, Yuen K. Characterization of a laboratory-generated variant of BPS beta-lactamase from Burkholderia pseudomallei that hydrolyses ceftazidime. J Antimicrob Chemother. 2002;50:723-6 pubmed
    ..coli conferred resistance to ceftazidime (MIC >or= 32 mg/L). The amino acid sequence of BPS-1m differed from that of BPS-1 by a Pro-to-Ser change at position 167 in the omega loop...
  25. Mahenthiralingam E, Baldwin A, Vandamme P. Burkholderia cepacia complex infection in patients with cystic fibrosis. J Med Microbiol. 2002;51:533-8 pubmed publisher
    ..For microbiologists, hospital infection control officers, caregivers, and most of all the CF community, the changes in our understanding of the taxonomy, epidemiology and pathogenesis of the bacterium 'B. cepacia' are complex...
  26. Vandamme P, Henry D, Coenye T, Nzula S, Vancanneyt M, LiPuma J, et al. Burkholderia anthina sp. nov. and Burkholderia pyrrocinia, two additional Burkholderia cepacia complex bacteria, may confound results of new molecular diagnostic tools. FEMS Immunol Med Microbiol. 2002;33:143-9 pubmed
    ..cepacia complex. This highlights another potential source for diagnostic problems with B. cepacia-like bacteria...
  27. Woo P, Leung P, Tsoi H, Yuen K. Cloning and characterisation of malE in Burkholderia pseudomallei. J Med Microbiol. 2001;50:330-8 pubmed
    ..Further studies on serodiagnosis of melioidosis with recombinant MBP should be performed...
  28. Valvano M, Messner P, Kosma P. Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides. Microbiology. 2002;148:1979-89 pubmed publisher