Bordetella pertussis Tohama I



Top Publications

  1. Relman D, Domenighini M, Tuomanen E, Rappuoli R, Falkow S. Filamentous hemagglutinin of Bordetella pertussis: nucleotide sequence and crucial role in adherence. Proc Natl Acad Sci U S A. 1989;86:2637-41 pubmed
    ..An internal in-frame deletion in fhaB, encompassing the RGD region, causes loss of B. pertussis-binding to ciliated eukaryotic cells, confirming a potential role for this protein in host-cell binding and infection. ..
  2. Yuk M, Harvill E, Miller J. The BvgAS virulence control system regulates type III secretion in Bordetella bronchiseptica. Mol Microbiol. 1998;28:945-59 pubmed
    ..pertussis and two strains of B. parapertussis. B. pertussis strain 18323 and an ovine isolate of B. parapertussis show significant transcription of the genes in vitro...
  3. Favre D, Cryz S, Viret J. Cloning of the recA gene of Bordetella pertussis and characterization of its product. Biochimie. 1991;73:235-44 pubmed
    ..Amino acid sequence comparisons revealed a high degree of structural conservation between prokaryotic RecA proteins. ..
  4. Mooi F, ter Avest A, van der Heide H. Structure of the Bordetella pertussis gene coding for the serotype 3 fimbrial subunit. FEMS Microbiol Lett. 1990;54:327-31 pubmed
    ..Peptides derived from the conserved regions may be incorporated into a future pertussis vaccine to induce antibodies which confer protection against strains producing different fimbrial serotypes. ..
  5. Brickman T, Armstrong S. Bordetella pertussis fur gene restores iron repressibility of siderophore and protein expression to deregulated Bordetella bronchiseptica mutants. J Bacteriol. 1995;177:268-70 pubmed
    ..The fur gene of Bordetella pertussis was cloned by genetic complementation of this deregulated phenotype and confirmed as fur by nucleotide sequence analysis. ..
  6. Gilles A, Sismeiro O, Munier H, Fabian H, Mantsch H, Surewicz W, et al. Structural and physico-chemical characteristics of Bordetella pertussis adenylate kinase, a tryptophan-containing enzyme. Eur J Biochem. 1993;218:921-7 pubmed
    ..coli showed that the kinetic and structural properties of the two enzymes are very similar. However, infrared spectroscopy has allowed to identify small but significant differences in the secondary structure of the two proteins. ..
  7. Beall B, Sanden G. A Bordetella pertussis fepA homologue required for utilization of exogenous ferric enterobactin. Microbiology. 1995;141 ( Pt 12):3193-205 pubmed
    ..These findings suggest that enterobactin produced by other respiratory flora could aid in the colonization of the respiratory tract by Bordetella species. ..
  8. Fry N, Neal S, Harrison T, Miller E, Matthews R, George R. Genotypic variation in the Bordetella pertussis virulence factors pertactin and pertussis toxin in historical and recent clinical isolates in the United Kingdom. Infect Immun. 2001;69:5520-8 pubmed
    ..The most striking difference in the United Kingdom isolates is that all 105 of the most recent circulating strains (from 1998 to 1999) are of a pertussis toxin type found in the United Kingdom WCV, i.e., ptxA(1)...
  9. Donato G, Hsia H, Green C, Hewlett E. Adenylate cyclase toxin (ACT) from Bordetella hinzii: characterization and differences from ACT of Bordetella pertussis. J Bacteriol. 2005;187:7579-88 pubmed
    ..pertussis ACT. These results demonstrate the expression of ACT by B. hinzii and represent the first characterization of a potential virulence factor of this organism. ..

More Information

Publications129 found, 100 shown here

  1. Maskell D, Morrissey P, Dougan G. Cloning and nucleotide sequence of the aroA gene of Bordetella pertussis. J Bacteriol. 1988;170:2467-71 pubmed
    ..The amino acid sequence derived from the nucleotide sequence shows homology with the published amino acid sequences of aroA gene products of other microorganisms. ..
  2. Antoine R, Raze D, Locht C. Genomics of Bordetella pertussis toxins. Int J Med Microbiol. 2000;290:301-5 pubmed publisher
    ..pertussis chromosome by homologous recombination. The reporter gene present in this vector allowed us to show that at least some of the bug genes are expressed...
  3. Huston W, Jennings M, McEwan A. The multicopper oxidase of Pseudomonas aeruginosa is a ferroxidase with a central role in iron acquisition. Mol Microbiol. 2002;45:1741-50 pubmed
    ..In view of the widespread distribution of the mco gene in bacteria, it is suggested that an iron acquisition mechanism involving multicopper oxidases may be an important and hitherto unrecognized feature of bacterial pathogenicity...
  4. Villarino Romero R, Bibova I, Cerny O, Vecerek B, Wald T, Benada O, et al. The Bordetella pertussis type III secretion system tip complex protein Bsp22 is not a protective antigen and fails to elicit serum antibody responses during infection of humans and mice. Infect Immun. 2013;81:2761-7 pubmed publisher
    ..pertussis 18323. Unlike for B. bronchiseptica, hence, the Bsp22 protein is nonimmunogenic, and/or the serum antibody response to it is suppressed, during B. pertussis infections of humans and mice. ..
  5. Cieplak W, Burnette W, Mar V, Kaljot K, Morris C, Chen K, et al. Identification of a region in the S1 subunit of pertussis toxin that is required for enzymatic activity and that contributes to the formation of a neutralizing antigenic determinant. Proc Natl Acad Sci U S A. 1988;85:4667-71 pubmed
    ..These findings identify the region of homology located near the amino terminus of S1 as an apparent enzymatic subsite and a potentially important antigenic determinant. ..
  6. Cortina G, Barbieri J. Role of tryptophan 26 in the NAD glycohydrolase reaction of the S-1 subunit of pertussis toxin. J Biol Chem. 1989;264:17322-8 pubmed
  7. Boyd A, Ross P, Conroy H, Mahon N, Lavelle E, Mills K. Bordetella pertussis adenylate cyclase toxin modulates innate and adaptive immune responses: distinct roles for acylation and enzymatic activity in immunomodulation and cell death. J Immunol. 2005;175:730-8 pubmed
  8. Antila M, He Q, de Jong C, Aarts I, Verbakel H, Bruisten S, et al. Bordetella holmesii DNA is not detected in nasopharyngeal swabs from Finnish and Dutch patients with suspected pertussis. J Med Microbiol. 2006;55:1043-51 pubmed publisher
    ..The results suggest that B. holmesii is not among the causative agents of pertussis-like symptoms in Finnish and Dutch patients and thus does not in practice confound IS481 and IS1001 PCRs...
  9. Selwa E, Laine E, Malliavin T. Differential role of calmodulin and calcium ions in the stabilization of the catalytic domain of adenyl cyclase CyaA from Bordetella pertussis. Proteins. 2012;80:1028-40 pubmed publisher
  10. Selwa E, Davi M, Chenal A, Sotomayor Pérez A, Ladant D, Malliavin T. Allosteric activation of Bordetella pertussis adenylyl cyclase by calmodulin: molecular dynamics and mutagenesis studies. J Biol Chem. 2014;289:21131-41 pubmed
    ..Overall, our results suggest that the loop-helix-loop motif at the C-terminal end of AC is crucial during CaM binding for stabilizing the AC catalytic loop in an active configuration. ..
  11. Beier D, Schwarz B, Fuchs T, Gross R. In vivo characterization of the unorthodox BvgS two-component sensor protein of Bordetella pertussis. J Mol Biol. 1995;248:596-610 pubmed publisher
    ..However, the BvgS output domain could not be substituted with output domains of the related proteins without inactivation of BvgS...
  12. Wohlkonig A, Hodak H, Clantin B, Senechal M, Bompard C, Jacob Dubuisson F, et al. Crystallization and preliminary X-ray diffraction analysis of the peptidylprolyl isomerase Par27 of Bordetella pertussis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008;64:809-12 pubmed publisher
    ..6, b = 214.1, c = 57.8 A), diffracts to 2.2 A resolution. Preliminary diffraction data analysis agreed with the presence of one monomer in the asymmetric unit of the orthorhombic crystal form and two in the monoclinic form. ..
  13. Asgarian Omran H, Amirzargar A, Zeerleder S, Mahdavi M, van Mierlo G, Solati S, et al. Interaction of Bordetella pertussis filamentous hemagglutinin with human TLR2: identification of the TLR2-binding domain. APMIS. 2015;123:156-62 pubmed publisher
    ..Interaction of FHA with TLR2 suggests its involvement in induction of the innate immune system against Bordetella pertussis. The TLR2-binding domain of FHA may contribute to immunoprotection against pertussis infection. ..
  14. Boulanger A, Moon K, Decker K, Chen Q, Knipling L, Stibitz S, et al. Bordetella pertussis fim3 gene regulation by BvgA: phosphorylation controls the formation of inactive vs. active transcription complexes. Proc Natl Acad Sci U S A. 2015;112:E526-35 pubmed publisher
  15. Fernandez R, Weiss A. Cloning and sequencing of the Bordetella pertussis cpn10/cpn60 (groESL) homolog. Gene. 1995;158:151-2 pubmed
    ..The promoter contains consensus sequences for both sigma 32 and sigma 70 binding, and it possesses the CIRCE regulatory inverted repeat. A potential Rho-independent terminator was identified and appears to be shared with the brkA gene. ..
  16. Westrop G, Hormozi E, Da Costa N, Parton R, Coote J. Bordetella pertussis adenylate cyclase toxin: proCyaA and CyaC proteins synthesised separately in Escherichia coli produce active toxin in vitro. Gene. 1996;180:91-9 pubmed
    ..The in vitro activation system may be used to investigate the mechanism of the CyaC-dependent acylation of proCyaA and the effect of variation of the modifying fatty acyl group on target cell specificity and toxic activity of CyaA...
  17. Wood G, Khelef N, Guiso N, Friedman R. Identification of Btr-regulated genes using a titration assay. Search for a role for this transcriptional regulator in the growth and virulence of Bordetella pertussis. Gene. 1998;209:51-8 pubmed
    ..Putative overlapping promoters and a Btr binding site (FNR box) were identified in the DNA sequence between brg1 and the adjacent genes. These intervening sequences may represent sites for regulation by Btr and Brg1...
  18. Cuzzoni A, Pedroni P, Riboli B, Grandi G, de Ferra F. Nucleotide sequence of the fim3 gene from Bordetella pertussis and homology to fim2 and fimX gene products. Nucleic Acids Res. 1990;18:1640 pubmed
  19. Fuchs T, Deppisch H, Scarlato V, Gross R. A new gene locus of Bordetella pertussis defines a novel family of prokaryotic transcriptional accessory proteins. J Bacteriol. 1996;178:4445-52 pubmed
    ..pertussis indicate that the members of this new protein family may play an important role in the transcription machinery of prokaryotic organisms...
  20. Farizo K, Cafarella T, Burns D. Evidence for a ninth gene, ptlI, in the locus encoding the pertussis toxin secretion system of Bordetella pertussis and formation of a PtlI-PtlF complex. J Biol Chem. 1996;271:31643-9 pubmed
    ..In B. pertussis, PtlI and PtlF co-precipitated with antibody to PtlF. These findings demonstrate the existence of PtlI and a PtlI-PtlF complex, providing the first description of an interaction between Ptl proteins. ..
  21. Brickman T, Armstrong S. Essential role of the iron-regulated outer membrane receptor FauA in alcaligin siderophore-mediated iron uptake in Bordetella species. J Bacteriol. 1999;181:5958-66 pubmed
    ..FauA is a 79-kDa iron-regulated outer membrane receptor protein required for transport and utilization of ferric alcaligin siderophore complexes by Bordetella species...
  22. Shina A, Hart C, Stenton M, Dawson S, McCracken C, Binns S, et al. Distribution of fim3 and flaA TTGE sequence types amongst isolates of Bordetella bronchiseptica from different host animals. J Med Microbiol. 2002;51:557-63 pubmed
    ..As both fimbriae and flagella have been implicated in adherence of bordetellae to host cells, sequence variations in fimbrial proteins and FlaA may have a role to play in host preferences...
  23. Hodak H, Clantin B, Willery E, Villeret V, Locht C, Jacob Dubuisson F. Secretion signal of the filamentous haemagglutinin, a model two-partner secretion substrate. Mol Microbiol. 2006;61:368-82 pubmed publisher
    ..The N-terminal domain of FhaC is involved in the FHA-FhaC interaction, in agreement with its proposed function and periplasmic localization...
  24. Drobnak I, Braselmann E, Clark P. Multiple driving forces required for efficient secretion of autotransporter virulence proteins. J Biol Chem. 2015;290:10104-16 pubmed publisher
    ..These results reconcile seemingly contradictory reports regarding the importance of passenger folding as a driving force for OM translocation but also reveal that another energy source is required to initiate translocation. ..
  25. Rivera Millot A, Lesne E, Solans L, Coutte L, Bertrand Michel J, Froguel P, et al. Characterization of a Bvg-regulated fatty acid methyl-transferase in Bordetella pertussis. PLoS ONE. 2017;12:e0176396 pubmed publisher
    ..The high level of conservation of BP2936 among B. pertussis isolates nevertheless argues that it probably plays a role in the life cycle of this pathogen. ..
  26. Scanlon K, Snyder Y, Skerry C, Carbonetti N. Fatal Pertussis in the Neonatal Mouse Model Is Associated with Pertussis Toxin-Mediated Pathology beyond the Airways. Infect Immun. 2017;85: pubmed publisher
    ..pertussis transmission in mice. This model will be a valuable tool to investigate causes of pertussis pathogenesis and identify potential therapies for critical pertussis. ..
  27. Li Z, Hannah J, Stibitz S, Nguyen N, Manclark C, Brennan M. Cloning and sequencing of the structural gene for the porin protein of Bordetella pertussis. Mol Microbiol. 1991;5:1649-56 pubmed
    ..DNA hybridization studies indicated that both virulent and avirulent strains of B. pertussis contain only one copy of this gene and that Bordetella bronchiseptica and Bordetella parapertussis contain a similar gene. ..
  28. Barry E, Weiss A, Ehrmann I, Gray M, Hewlett E, Goodwin M. Bordetella pertussis adenylate cyclase toxin and hemolytic activities require a second gene, cyaC, for activation. J Bacteriol. 1991;173:720-6 pubmed
    ..Sequence analysis of the upstream region defined an open reading frame with homology to the E. coli hlyC gene. In contrast to E. coli, this open reading frame is oriented oppositely from the adenylate cyclase toxin structural gene...
  29. Pizza M, Bugnoli M, Pucci P, Siciliano R, Marino G, Rappuoli R. Further analysis of the sequence of the S1 subunit of pertussis toxin. Infect Immun. 1991;59:1177-9 pubmed
    ..In this report, we provide evidence that Bordetella pertussis strains are able to produce active pertussis toxin only when they contain one of the two possible nucleotide sequences. ..
  30. Charles I, Dougan G, Pickard D, Chatfield S, Smith M, Novotny P, et al. Molecular cloning and characterization of protective outer membrane protein P.69 from Bordetella pertussis. Proc Natl Acad Sci U S A. 1989;86:3554-8 pubmed
    ..69 carried by the cosmid pBPI69 was unable to direct the expression of P.69 protein in an Escherichia coli host. The generation of P.69-fusion products allowed the detection of P.69-specific protein products synthesized in E. coli...
  31. Carbonetti N, Fuchs T, Patamawenu A, Irish T, Deppisch H, Gross R. Effect of mutations causing overexpression of RNA polymerase alpha subunit on regulation of virulence factors in Bordetella pertussis. J Bacteriol. 1994;176:7267-73 pubmed
    ..pertussis, which caused the same phenotype as the mutants showed. Conclusions in regard to the identity of the transcription activator of the toxin genes are discussed. ..
  32. Mooi F, van Oirschot H, Heuvelman K, van der Heide H, Gaastra W, Willems R. Polymorphism in the Bordetella pertussis virulence factors P.69/pertactin and pertussis toxin in The Netherlands: temporal trends and evidence for vaccine-driven evolution. Infect Immun. 1998;66:670-5 pubmed
    ..Our findings cast a new light on the reemergence of pertussis in highly vaccinated populations and may have major implications for the long-term efficacy of both WCVs and ACVs...
  33. Craig Mylius K, Weiss A. Mutants in the ptlA-H genes of Bordetella pertussis are deficient for pertussis toxin secretion. FEMS Microbiol Lett. 1999;179:479-84 pubmed
    ..The mutations were also introduced into the adenylate cyclase locus of a wild-type ptxptl strain, resulting in a ptl diploid strain. The PtlC, PtlD, PtlE, PtlF, PtlG and PtlH mutants exerted dominance over the wild-type allele...
  34. Rambow Larsen A, Weiss A. The PtlE protein of Bordetella pertussis has peptidoglycanase activity required for Ptl-mediated pertussis toxin secretion. J Bacteriol. 2002;184:2863-9 pubmed
    ..Based on these results, we concluded that PtlE is a peptidoglycanase responsible for the local removal or rearrangement of the peptidoglycan layer during Ptl secretion complex assembly...
  35. Wang G, Wang S, Gentles R, Sowin T, Maring C, Kempf D, et al. Design, synthesis, and structural analysis of inhibitors of influenza neuraminidase containing a 2,3-disubstituted tetrahydrofuran-5-carboxylic acid core. Bioorg Med Chem Lett. 2005;15:125-8 pubmed
    ..Both compounds 9 and 14 inhibit influenza NA A with an IC(50) of about 0.5 microM and NA B with an IC(50) of 1.0 microM. ..
  36. Zocchi M, Contini P, Alfano M, Poggi A. Pertussis toxin (PTX) B subunit and the nontoxic PTX mutant PT9K/129G inhibit Tat-induced TGF-beta production by NK cells and TGF-beta-mediated NK cell apoptosis. J Immunol. 2005;174:6054-61 pubmed
    ..Together, these data suggest that Tat-induced TGF-beta production and the consequent NK cell failure, possibly occurring during early HIV-1 infection, may be regulated by PTX-B and PT9K/129G...
  37. Rossi P, Theresa Ramelot S, Xiao R, Ho C, Ma L, Acton T, et al. 1H, 13C, and 15N resonance assignments for the protein coded by gene locus BB0938 of Bordetella bronchiseptica. J Biomol NMR. 2005;33:197 pubmed
  38. Fiser R, Mas n J, Basler M, Krusek J, Spul kov V, Konop sek I, et al. Third activity of Bordetella adenylate cyclase (AC) toxin-hemolysin. Membrane translocation of AC domain polypeptide promotes calcium influx into CD11b+ monocytes independently of the catalytic and hemolytic activities. J Biol Chem. 2007;282:2808-20 pubmed publisher
    ..Hence, the translocating AC polypeptide itself appears to participate in formation of a novel type of membrane path for calcium ions, contributing to action of CyaA in an unexpected manner...
  39. Hasan S, Osickova A, Bumba L, Novák P, Sebo P, Osicka R. Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding. FEBS Lett. 2015;589:374-9 pubmed publisher
    ..We suggest that these N-glycans form a defined clustered saccharide patch that enables multivalent contact of CR3 with CyaA, enhancing both affinity and specificity of the integrin-toxin interaction. ..
  40. Finn T, Li Z, Kocsis E. Identification of a Bordetella pertussis bvg-regulated porin-like protein. J Bacteriol. 1995;177:805-9 pubmed
    ..The results of Southern blot analysis indicate that strains of Bordetella parapertussis and Bordetella bronchiseptica but not Bordetella avium contain this gene. ..
  41. Willems R, Geuijen C, van der Heide H, Renauld G, Bertin P, van den Akker W, et al. Mutational analysis of the Bordetella pertussis fim/fha gene cluster: identification of a gene with sequence similarities to haemolysin accessory genes involved in export of FHA. Mol Microbiol. 1994;11:337-47 pubmed
    ..These results suggest that the N-terminus of FHA interacts with FhaC, and that as a result FHA is transported across the outer membrane...
  42. Coutte L, Antoine R, Drobecq H, Locht C, Jacob Dubuisson F. Subtilisin-like autotransporter serves as maturation protease in a bacterial secretion pathway. EMBO J. 2001;20:5040-8 pubmed publisher
    ..SphB1 is the first described subtilisin-like protein that serves as a specialized maturation protease in a secretion pathway of Gram-negative bacteria. This is reminiscent of pro-protein convertases of eukaryotic cells...
  43. Clantin B, Hodak H, Willery E, Locht C, Jacob Dubuisson F, Villeret V. The crystal structure of filamentous hemagglutinin secretion domain and its implications for the two-partner secretion pathway. Proc Natl Acad Sci U S A. 2004;101:6194-9 pubmed publisher
    ..This structure is representative of the TPS domains found so far in >100 secreted proteins from pathogenic bacteria. It also provides a mechanistic insight into how protein folding may be linked to secretion in the TPS pathway...
  44. Fiser R, Konopasek I. Different modes of membrane permeabilization by two RTX toxins: HlyA from Escherichia coli and CyaA from Bordetella pertussis. Biochim Biophys Acta. 2009;1788:1249-54 pubmed publisher
    ..We concluded that the fluorescence requenching method in combination with different size of liposomes is a valuable tool for characterization of pore-forming toxins and their variants. ..
  45. Subrini O, Sotomayor P rez A, Hessel A, Spiaczka Karst J, Selwa E, Sapay N, et al. Characterization of a membrane-active peptide from the Bordetella pertussis CyaA toxin. J Biol Chem. 2013;288:32585-98 pubmed publisher
  46. Boucher P, Stibitz S. Synergistic binding of RNA polymerase and BvgA phosphate to the pertussis toxin promoter of Bordetella pertussis. J Bacteriol. 1995;177:6486-91 pubmed
    ..The implications of these observations for pertussis toxin expression and regulation are discussed...
  47. Schneider O, Weiss A, Miller W. Pertussis toxin signals through the TCR to initiate cross-desensitization of the chemokine receptor CXCR4. J Immunol. 2009;182:5730-9 pubmed publisher
    ..These data suggest that TCR crosstalk with CXCR4 is likely a normal cellular process that leads to cross-desensitization, which is exploited by the B subunit of PTx...
  48. El Kady E, Abu Shady H, Tohamy H, Wahby A. The preparation of A purified immunogenic pertactin from B. pertussis. Egypt J Immunol. 2007;14:11-8 pubmed
    ..9 and retains a high immunogenicity. The rabbit anti- pertactin antisera shows high specificity in ELISA. The purified pertactin is a potential candidate as immunogen for preparation of diagnostic reagents and as a vaccine component. ..
  49. Bibova I, Hot D, Keidel K, Amman F, Slupek S, Cerny O, et al. Transcriptional profiling of Bordetella pertussis reveals requirement of RNA chaperone Hfq for Type III secretion system functionality. RNA Biol. 2015;12:175-85 pubmed publisher
    ..Importantly, for the first time we demonstrate that B. pertussis T3SS can be activated upon contact with macrophage cells in vitro. ..
  50. Locht C, Geoffroy M, Renauld G. Common accessory genes for the Bordetella pertussis filamentous hemagglutinin and fimbriae share sequence similarities with the papC and papD gene families. EMBO J. 1992;11:3175-83 pubmed
    ..Deletion analyses suggest that fhaB and the downstream genes can be transcribed as a polycistronic operon, and primer extension analysis revealed the presence of a second promoter between fhaB and fhaD...
  51. Glaser P, Munier H, Gilles A, Krin E, Porumb T, Barzu O, et al. Functional consequences of single amino acid substitutions in calmodulin-activated adenylate cyclase of Bordetella pertussis. EMBO J. 1991;10:1683-8 pubmed
  52. Loosmore S, Cunningham J, Bradley W, Yao F, Dekaban G, Klein M. A unique sequence of the Bordetella pertussis toxin operon. Nucleic Acids Res. 1989;17:8365 pubmed
  53. Cockle S. Identification of an active-site residue in subunit S1 of pertussis toxin by photocrosslinking to NAD. FEBS Lett. 1989;249:329-32 pubmed
    ..Sequence determination of purified radioactive peptides indicated that Glu-129 was a major site of labelling. This residue is therefore closely associated with either NAD binding or hydrolysis. ..
  54. DeShazer D, Wood G, Friedman R. Molecular characterization of catalase from Bordetella pertussis: identification of the katA promoter in an upstream insertion sequence. Mol Microbiol. 1994;14:123-30 pubmed
    ..However, there was no difference in the ability of DD900 and BP339 to survive for 2 h in human PMNs. This suggests that catalase plays no significant role in the survival of B. pertussis within PMNs...
  55. Willems R, Geuijen C, van der Heide H, Matheson M, Robinson A, Versluis L, et al. Isolation of a putative fimbrial adhesin from Bordetella pertussis and the identification of its gene. Mol Microbiol. 1993;9:623-34 pubmed
  56. Ricci S, Rappuoli R, Scarlato V. The pertussis toxin liberation genes of Bordetella pertussis are transcriptionally linked to the pertussis toxin operon. Infect Immun. 1996;64:1458-60 pubmed
    ..Deletion of the 55-bp DNA region caused an increase in the amount of the ptl transcripts. It is likely that this DNA region is involved in regulation of the ptx-pti expression. ..
  57. Smith A, Yan H, Groves N, Dalla Pozza T, Walker M. Co-expression of the Bordetella pertussis leader peptidase I results in enhanced processing and expression of the pertussis toxin S1 subunit in Escherichia coli. FEMS Microbiol Lett. 2000;191:177-82 pubmed
    ..Co-expression of the B. pertussis lep gene in E. coli strain TOPP2 expressing the pertussis toxin S1 subunit was found to markedly increase the expression and post-translational processing of the S1 protein...
  58. Van Loo I, Heuvelman K, King A, Mooi F. Multilocus sequence typing of Bordetella pertussis based on surface protein genes. J Clin Microbiol. 2002;40:1994-2001 pubmed
    ..These types may represent newly emerged, successful clones. The identification of highly successful clones may shed light on the question of how B. pertussis is able to maintain itself in vaccinated populations...
  59. Villarino Romero R, Osicka R, Sebo P. Filamentous hemagglutinin of Bordetella pertussis: a key adhesin with immunomodulatory properties?. Future Microbiol. 2014;9:1339-60 pubmed publisher
    ..Here we review the state of knowledge on this important virulence factor and acellular pertussis vaccine component. Specific emphasis is placed on outstanding questions that are yet to be answered. ..
  60. Delisse Gathoye A, Locht C, Jacob F, Raaschou Nielsen M, Heron I, Ruelle J, et al. Cloning, partial sequence, expression, and antigenic analysis of the filamentous hemagglutinin gene of Bordetella pertussis. Infect Immun. 1990;58:2895-905 pubmed
    ..The reactivities of several recombinant FHA-derived proteins with a panel of monoclonal antibodies identified at least four epitopes composing an immunoreactive domain present in the carboxy-terminal moiety of the mature FHA...
  61. Emsley P, Charles I, Fairweather N, Isaacs N. Structure of Bordetella pertussis virulence factor P.69 pertactin. Nature. 1996;381:90-2 pubmed publisher
    ..The carboxy-terminal region of P.69 pertactin incorporates a (PQP)5 motif loop containing the major immunoprotective epitope...
  62. Pullinger G, Adams T, Mullan P, Garrod T, Lax A. Cloning, expression, and molecular characterization of the dermonecrotic toxin gene of Bordetella spp. Infect Immun. 1996;64:4163-71 pubmed
    ..Extracts containing the recombinant or the native toxin induced DNA synthesis in Swiss 3T3 cells but inhibited cell division leading to binucleation...
  63. Steffen P, Goyard S, Ullmann A. The Bordetella pertussis sigma subunit of RNA polymerase confers enhanced expression of fha in Escherichia coli. Mol Microbiol. 1997;23:945-54 pubmed
    ..coli UQ285 strains carrying the B. pertussis bvg locus. We discuss the implications of these results on the mechanisms involved in the activation of virulence-associated promoters...
  64. Pradel E, Guiso N, Menozzi F, Locht C. Bordetella pertussis TonB, a Bvg-independent virulence determinant. Infect Immun. 2000;68:1919-27 pubmed
    ..Nevertheless, in vivo in the mouse respiratory infection model, the colonization ability of the mutant was reduced compared to the parental strain...
  65. West N, Jungnitz H, Fitter J, McArthur J, Guzm n C, Walker M. Role of phosphoglucomutase of Bordetella bronchiseptica in lipopolysaccharide biosynthesis and virulence. Infect Immun. 2000;68:4673-80 pubmed
    ..In contrast, the inability to produce wild-type LPS resulted in a reduced bacterial resistance to oxidative stress and a higher susceptibility to the antimicrobial peptide cecropin P...
  66. King A, Berbers G, van Oirschot H, Hoogerhout P, Knipping K, Mooi F. Role of the polymorphic region 1 of the Bordetella pertussis protein pertactin in immunity. Microbiology. 2001;147:2885-95 pubmed publisher
    ..An mAb directed against a linear conserved epitope conferred cross-immunity against isolates with distinct pertactin variants. The results indicate an important role of region 1 of pertactin in immunity...
  67. Brickman T, Armstrong S. Bordetella AlcS transporter functions in alcaligin siderophore export and is central to inducer sensing in positive regulation of alcaligin system gene expression. J Bacteriol. 2005;187:3650-61 pubmed publisher
  68. M li A, Hodak H, Clantin B, Locht C, Molle G, Jacob Dubuisson F, et al. Channel properties of TpsB transporter FhaC point to two functional domains with a C-terminal protein-conducting pore. J Biol Chem. 2006;281:158-66 pubmed publisher
    ..The N-terminal 200 residues of FhaC constitute a functionally distinct domain that modulates the pore properties and may participate in FHA recognition...
  69. Huvent I, Belrhali H, Antoine R, Bompard C, Locht C, Jacob Dubuisson F, et al. Crystal structure of Bordetella pertussis BugD solute receptor unveils the basis of ligand binding in a new family of periplasmic binding proteins. J Mol Biol. 2006;356:1014-26 pubmed publisher
    ..The present structure thus unveils the bases of ligand binding in this large family of periplasmic binding proteins, several hundred members of which have been identified in various bacterial species...
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    ..These toxin analogues constitute thus interesting probes for the study of cellular functions, in particular immune cell functions, for which natural PTX has already shown its usefulness...
  71. Brickman T, Armstrong S. Iron and pH-responsive FtrABCD ferrous iron utilization system of Bordetella species. Mol Microbiol. 2012;86:580-93 pubmed publisher
    ..The pH of human airway surface fluids ranges from pH?5.5 to 7.9, and the FtrABCD system may supply ferrous iron necessary for Bordetella growth in acidic host microenvironments in which siderophores are ineffective for iron retrieval...
  72. Li J, Fairweather N, Novotny P, Dougan G, Charles I. Cloning, nucleotide sequence and heterologous expression of the protective outer-membrane protein P.68 pertactin from Bordetella bronchiseptica. J Gen Microbiol. 1992;138 Pt 8:1697-705 pubmed publisher
    ..The major differences between all three proteins occur in the number of repeats of the two families (Gly-Gly-Xaa-Xaa-Pro)n and (Pro-Gln-Pro)n of reiterated sequence motifs...
  73. Munier H, Gilles A, Glaser P, Krin E, Danchin A, Sarfati R, et al. Isolation and characterization of catalytic and calmodulin-binding domains of Bordetella pertussis adenylate cyclase. Eur J Biochem. 1991;196:469-74 pubmed
    ..Reassociation was enhanced by calmodulin itself, which 'trapped' the two complementary peptides into a stable, native-like, ternary complex, which shows similar catalytic properties to intact adenylate cyclase...
  74. Covacci A, Rappuoli R. Pertussis toxin export requires accessory genes located downstream from the pertussis toxin operon. Mol Microbiol. 1993;8:429-34 pubmed
    ..This is a novel mechanism of protein export in Gram-negative bacteria...
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    ..Thus a set of accessory proteins are most likely involved in the secretion of pertussis toxin, and these proteins appear to be members of a family of proteins involved in the secretion of macromolecules from bacteria...
  76. Uhl M, Miller J. Integration of multiple domains in a two-component sensor protein: the Bordetella pertussis BvgAS phosphorelay. EMBO J. 1996;15:1028-36 pubmed
    ..Activity of BvgS His 1172-->Gln could be restored by providing the wild-type C-terminal domain in trans. Our results indicate an obligatory role for an alternate phosphodonor module in the BvgAS phosphorelay...
  77. Hazes B, Boodhoo A, Cockle S, Read R. Crystal structure of the pertussis toxin-ATP complex: a molecular sensor. J Mol Biol. 1996;258:661-71 pubmed publisher
    ..The key features of this proposal are that ATP binding signals the arrival of the toxin in the endoplasmic reticulum and, at the same time, triggers dissociation of the holotoxin prior to membrane translocation...
  78. Allen A, Isobe T, Maskell D. Identification and cloning of waaF (rfaF) from Bordetella pertussis and use to generate mutants of Bordetella spp. with deep rough lipopolysaccharide. J Bacteriol. 1998;180:35-40 pubmed
    ..These are the first LPS mutants generated in B. parapertussis and B. bronchiseptica and the first deep rough mutants of any of the bordetellae...
  79. Boursaux Eude C, Thiberge S, Carletti G, Guiso N. Intranasal murine model of Bordetella pertussis infection: II. Sequence variation and protection induced by a tricomponent acellular vaccine. Vaccine. 1999;17:2651-60 pubmed
    ..pertussis variants...
  80. Nicholson M, Beall B. Disruption of tonB in Bordetella bronchiseptica and Bordetella pertussis prevents utilization of ferric siderophores, haemin and haemoglobin as iron sources. Microbiology. 1999;145 ( Pt 9):2453-61 pubmed
    ..pertussis fepA homologue and tonB(Bb) operon...
  81. Perraud A, Rippe K, Bantscheff M, Glocker M, Lucassen M, Jung K, et al. Dimerization of signalling modules of the EvgAS and BvgAS phosphorelay systems. Biochim Biophys Acta. 2000;1478:341-54 pubmed
    ..In agreement with these data, surface plasmon resonance studies revealed a 2:1 stoichiometry in the interaction of EvgA with the immobilized EvgS HPt domain and an affinity constant of 1. 24x10(6) M(-1)...
  82. Gu din S, Willery E, Tommassen J, Fort E, Drobecq H, Locht C, et al. Novel topological features of FhaC, the outer membrane transporter involved in the secretion of the Bordetella pertussis filamentous hemagglutinin. J Biol Chem. 2000;275:30202-10 pubmed publisher
    ..The surface exposure of the N terminus and the presence of an odd number of beta-strands are novel features for beta-barrel outer membrane proteins...
  83. Stenson T, Weiss A. DsbA and DsbC are required for secretion of pertussis toxin by Bordetella pertussis. Infect Immun. 2002;70:2297-303 pubmed
    ..Mutations in dsbC did not impair assembly of periplasmic toxin but resulted in decreased toxin secretion, suggesting a defect in the formation of the Ptl secretion complex...
  84. Vanderpool C, Armstrong S. Heme-responsive transcriptional activation of Bordetella bhu genes. J Bacteriol. 2003;185:909-17 pubmed
    ..Furthermore, bhuR was required for heme-inducible bhu gene activation, supporting the hypothesis that positive regulation of bhuRSTUV occurs by a surface signaling mechanism involving the heme-iron receptor BhuR...
  85. Antoine R, Jacob Dubuisson F, Drobecq H, Willery E, Lesjean S, Locht C. Overrepresentation of a gene family encoding extracytoplasmic solute receptors in Bordetella. J Bacteriol. 2003;185:1470-4 pubmed
    ..pertussis, and one is involved in citrate uptake. The bug genes probably form an ancient family that has been subjected to a large expansion in a restricted phylogenic group...
  86. Bauche C, Chenal A, Knapp O, Bodenreider C, Benz R, Chaffotte A, et al. Structural and functional characterization of an essential RTX subdomain of Bordetella pertussis adenylate cyclase toxin. J Biol Chem. 2006;281:16914-26 pubmed publisher
    ..Upon calcium binding to the RTX repeats, a conformational rearrangement of the adjacent non-RTX sequences may act as a critical molecular switch to trigger the CyaA entry into target cells...
  87. Perkins D, Gray M, Hewlett E, Vogel S. Bordetella pertussis adenylate cyclase toxin (ACT) induces cyclooxygenase-2 (COX-2) in murine macrophages and is facilitated by ACT interaction with CD11b/CD18 (Mac-1). Mol Microbiol. 2007;66:1003-15 pubmed publisher
    ..pertussis infection by augmenting COX-2 expression and provides evidence against the concept that ACT functions exclusively via its inhibitory effects on phagocytic leucocytes...
  88. Morova J, Osicka R, Masin J, Sebo P. RTX cytotoxins recognize beta2 integrin receptors through N-linked oligosaccharides. Proc Natl Acad Sci U S A. 2008;105:5355-60 pubmed publisher
    ..These results show that binding and killing of target cells by CyaA, LtxA, and HlyA depends on recognition of N-linked oligosaccharide chains of beta(2) integrin receptors. This sets a new paradigm for action of RTX cytotoxins...
  89. G ttle M, Dove S, Kees F, Schlossmann J, Geduhn J, K nig B, et al. Cytidylyl and uridylyl cyclase activity of bacillus anthracis edema factor and Bordetella pertussis CyaA. Biochemistry. 2010;49:5494-503 pubmed publisher
    ..In conclusion, we have shown both EF and CyaA have a rather broad substrate specificity and exhibit cytidylyl and uridylyl cyclase activity. Both cCMP and cUMP may contribute to toxin actions...
  90. Dunne A, Ross P, Pospisilova E, Masin J, Meaney A, Sutton C, et al. Inflammasome activation by adenylate cyclase toxin directs Th17 responses and protection against Bordetella pertussis. J Immunol. 2010;185:1711-9 pubmed publisher
    ..In addition to its known role in subverting host immunity, our findings suggest that CyaA can promote IL-1beta-mediated Th17 cells, which promote clearance of the bacteria from the respiratory tract...
  91. Raksanoh V, Shank L, Prangkio P, Yentongchai M, Sakdee S, Imtong C, et al. Zn2+-dependent autocatalytic activity of the Bordetella pertussis CyaA-hemolysin. Biochem Biophys Res Commun. 2017;485:720-724 pubmed publisher
    ..Overall results demonstrated for the first time that the observed proteolysis of CyaA-HP/BI and CyaA-RTX fragments is conceivably due to their Zn2+-dependent autocatalytic activity. ..
  92. Gentry Weeks C, Hultsch A, Kelly S, Keith J, Curtiss R. Cloning and sequencing of a gene encoding a 21-kilodalton outer membrane protein from Bordetella avium and expression of the gene in Salmonella typhimurium. J Bacteriol. 1992;174:7729-42 pubmed
    ..avium 197 resulted in colonization of the trachea and thymus with B. avium 197...