Streptococcus pneumoniae R6


Alias: Streptococcus pneumoniae (strain ATCC BAA-255 / R6), Streptococcus pneumoniae str. R6

Top Publications

  1. Attaiech L, Olivier A, Mortier Barrière I, Soulet A, Granadel C, Martin B, et al. Role of the single-stranded DNA-binding protein SsbB in pneumococcal transformation: maintenance of a reservoir for genetic plasticity. PLoS Genet. 2011;7:e1002156 pubmed publisher
    ..pneumoniae by increasing the likelihood of multiple transformation events in the same cell. ..
  2. Salles C, Creancier L, Claverys J, Mejean V. The high level streptomycin resistance gene from Streptococcus pneumoniae is a homologue of the ribosomal protein S12 gene from Escherichia coli. Nucleic Acids Res. 1992;20:6103 pubmed
  3. Martin B, Humbert O, Camara M, Guenzi E, Walker J, Mitchell T, et al. A highly conserved repeated DNA element located in the chromosome of Streptococcus pneumoniae. Nucleic Acids Res. 1992;20:3479-83 pubmed
    ..pneumoniae. This location raises the intriguing possibility that BOX sequences are regulatory elements shared by several coordinately controlled genes, including competence-specific and virulence-related genes. ..
  4. Havarstein L, Coomaraswamy G, Morrison D. An unmodified heptadecapeptide pheromone induces competence for genetic transformation in Streptococcus pneumoniae. Proc Natl Acad Sci U S A. 1995;92:11140-4 pubmed
    ..The hypothesis is presented that this transport protein is encoded by comA, previously shown to be required for elaboration of the pneumococcal competence activator. ..
  5. Martin B, Garcia P, Castanié M, Claverys J. The recA gene of Streptococcus pneumoniae is part of a competence-induced operon and controls lysogenic induction. Mol Microbiol. 1995;15:367-79 pubmed
    ..This is unlike the situation of a recA+ lysogen. Together these results demonstrate that RecA controls lysogenic induction and suggest the existence of a SOS repair system in S. pneumoniae. ..
  6. Zhou L, Hui F, Morrison D. Characterization of IS1167, a new insertion sequence in Streptococcus pneumoniae. Plasmid. 1995;33:127-38 pubmed
  7. Hui F, Zhou L, Morrison D. Competence for genetic transformation in Streptococcus pneumoniae: organization of a regulatory locus with homology to two lactococcin A secretion genes. Gene. 1995;153:25-31 pubmed
  8. Puyet A, Ibanez A, Espinosa M. Characterization of the Streptococcus pneumoniae maltosaccharide regulator MalR, a member of the LacI-GalR family of repressors displaying distinctive genetic features. J Biol Chem. 1993;268:25402-8 pubmed
    ..The significance of these similarities is discussed in terms of the possible evolutionary pathways followed by structural and regulatory genes of sugar utilization systems in bacteria. ..
  9. Puyet A, Espinosa M. Structure of the maltodextrin-uptake locus of Streptococcus pneumoniae. Correlation to the Escherichia coli maltose regulon. J Mol Biol. 1993;230:800-11 pubmed
    ..The processing of mRNA has not been reported in the Gram-negative maltose regulons, and may suggest either a less evolved or a divergent system for the control of gene expression of this regulon in S. pneumoniae...

More Information

Publications146 found, 100 shown here

  1. Pestova E, Havarstein L, Morrison D. Regulation of competence for genetic transformation in Streptococcus pneumoniae by an auto-induced peptide pheromone and a two-component regulatory system. Mol Microbiol. 1996;21:853-62 pubmed
    ..Disruption of comE abolished both response to synthetic activator peptide and endogenous competence induction. ..
  2. Grebe T, Paik J, Hakenbeck R. A novel resistance mechanism against beta-lactams in Streptococcus pneumoniae involves CpoA, a putative glycosyltransferase. J Bacteriol. 1997;179:3342-9 pubmed
    ..This suggests that the cpo operon has a similar function related to cell surface components. ..
  3. Morona J, Morona R, Paton J. Characterization of the locus encoding the Streptococcus pneumoniae type 19F capsular polysaccharide biosynthetic pathway. Mol Microbiol. 1997;23:751-63 pubmed
    ..pneumoniae serotypes/groups tested. The region from cps19fG to cps19fK was found only in members of serogroup 19, and, within this, cps19fl was unique to type 19F...
  4. Pestova E, Morrison D. Isolation and characterization of three Streptococcus pneumoniae transformation-specific loci by use of a lacZ reporter insertion vector. J Bacteriol. 1998;180:2701-10 pubmed
    ..Genes at all three loci were confirmed to be required for transformation by mutagenesis using pEVP3 for insertion duplications or an erm cassette for gene disruptions. ..
  5. Ramirez M, Tomasz A. Molecular characterization of the complete 23F capsular polysaccharide locus of Streptococcus pneumoniae. J Bacteriol. 1998;180:5273-8 pubmed
    ..The 18.6-kb cps23f locus is composed of 18 open reading frames flanked at the 5' and 3' ends by the genes dexB and aliA, an arrangement similar to those of some of the other identified cps loci. ..
  6. Mollerach M, L pez R, Garc a E. Characterization of the galU gene of Streptococcus pneumoniae encoding a uridine diphosphoglucose pyrophosphorylase: a gene essential for capsular polysaccharide biosynthesis. J Exp Med. 1998;188:2047-56 pubmed
  7. Massidda O, Anderluzzi D, Friedli L, Feger G. Unconventional organization of the division and cell wall gene cluster of Streptococcus pneumoniae. Microbiology. 1998;144 ( Pt 11):3069-78 pubmed
  8. Garcia P, Gonzalez M, Garcia E, Lopez R, Garcia J. LytB, a novel pneumococcal murein hydrolase essential for cell separation. Mol Microbiol. 1999;31:1275-81 pubmed
  9. Zhang J, Idanpaan Heikkila I, Fischer W, Tuomanen E. Pneumococcal licD2 gene is involved in phosphorylcholine metabolism. Mol Microbiol. 1999;31:1477-88 pubmed
    ..Identification of the lic genes in the pneumococcus will facilitate further characterization of the role of surface choline in microbial physiology and pathogenesis. ..
  10. Lee M, Morrison D. Identification of a new regulator in Streptococcus pneumoniae linking quorum sensing to competence for genetic transformation. J Bacteriol. 1999;181:5004-16 pubmed
  11. Auzat I, Chapuy Regaud S, Le Bras G, Dos Santos D, Ogunniyi A, Le Thomas I, et al. The NADH oxidase of Streptococcus pneumoniae: its involvement in competence and virulence. Mol Microbiol. 1999;34:1018-28 pubmed
    ..This work establishes, for the first time, the role of O2 in the regulation of pneumococcal transformability and virulence. ..
  12. Yesilkaya H, Kadioglu A, Gingles N, Alexander J, Mitchell T, Andrew P. Role of manganese-containing superoxide dismutase in oxidative stress and virulence of Streptococcus pneumoniae. Infect Immun. 2000;68:2819-26 pubmed
    ..After infection with D39HY1, neutrophils were densely packed around bronchioles, in contrast to the wild-type infection, where neutrophils were more diffusely localized. ..
  13. Tait Kamradt A, Davies T, Cronan M, Jacobs M, Appelbaum P, Sutcliffe J. Mutations in 23S rRNA and ribosomal protein L4 account for resistance in pneumococcal strains selected in vitro by macrolide passage. Antimicrob Agents Chemother. 2000;44:2118-25 pubmed
    ..To our knowledge, this is the first description of mutations in 23S rRNA genes or ribosomal proteins in macrolide-resistant S. pneumoniae strains. ..
  14. Rieux V, Carbon C, Azoulay Dupuis E. Complex relationship between acquisition of beta-lactam resistance and loss of virulence in Streptococcus pneumoniae. J Infect Dis. 2001;184:66-72 pubmed
    ..2b and 23.2b.2x mutants had no change in MIC. Compensatory mutations are implicated in the revival of virulence. ..
  15. Echenique J, Trombe M. Competence repression under oxygen limitation through the two-component MicAB signal-transducing system in Streptococcus pneumoniae and involvement of the PAS domain of MicB. J Bacteriol. 2001;183:4599-608 pubmed
    ..It is proposed that MicAB, with its PAS motif, may belong to a set of functions important in the protection of the cell against oxidative stress, including the control of competence. ..
  16. Canu A, Malbruny B, Coquemont M, Davies T, Appelbaum P, Leclercq R. Diversity of ribosomal mutations conferring resistance to macrolides, clindamycin, streptogramin, and telithromycin in Streptococcus pneumoniae. Antimicrob Agents Chemother. 2002;46:125-31 pubmed
    ..In six remaining mutants, no mutations in L22, L4, or 23S rRNA could be detected. ..
  17. Pernot L, Chesnel L, Le Gouellec A, Croize J, Vernet T, Dideberg O, et al. A PBP2x from a clinical isolate of Streptococcus pneumoniae exhibits an alternative mechanism for reduction of susceptibility to beta-lactam antibiotics. J Biol Chem. 2004;279:16463-70 pubmed
    ..Interestingly, the active site of 5259-PBP2x is in closed conformation, whereas that of Sp328-PBP2x is open. Consequently, S. pneumoniae has evolved to employ the same protein in two distinct mechanisms of antibiotic resistance. ..
  18. Grove D, Bryant F. Effect of Mg2+ on the DNA binding modes of the Streptococcus pneumoniae SsbA and SsbB proteins. J Biol Chem. 2006;281:2087-94 pubmed
    ..Additional experiments with a chimeric SsbA/B protein indicated that the structural determinants that govern the transitions between the different DNA binding modes may be contained within the N-terminal domains of the SSB proteins. ..
  19. Beiter K, Wartha F, Albiger B, Normark S, Zychlinsky A, Henriques Normark B. An endonuclease allows Streptococcus pneumoniae to escape from neutrophil extracellular traps. Curr Biol. 2006;16:401-7 pubmed
    ..Furthermore, we demonstrate that escaping NETs promotes spreading of pneumococci from the upper airways to the lungs and from the lungs into the bloodstream during pneumonia. ..
  20. Trzcinski K, Thompson C, Gilbey A, Dowson C, Lipsitch M. Incremental increase in fitness cost with increased beta -lactam resistance in pneumococci evaluated by competition in an infant rat nasal colonization model. J Infect Dis. 2006;193:1296-303 pubmed
    ..The cost of penicillin-resistance acquisition for the Streptococcus pneumoniae strain competing with its susceptible ancestor to colonize the URT increases with the number of resistant pbp alleles acquired. ..
  21. Desai B, Morrison D. An unstable competence-induced protein, CoiA, promotes processing of donor DNA after uptake during genetic transformation in Streptococcus pneumoniae. J Bacteriol. 2006;188:5177-86 pubmed
  22. Kovács M, Halfmann A, Fedtke I, Heintz M, Peschel A, Vollmer W, et al. A functional dlt operon, encoding proteins required for incorporation of d-alanine in teichoic acids in gram-positive bacteria, confers resistance to cationic antimicrobial peptides in Streptococcus pneumoniae. J Bacteriol. 2006;188:5797-805 pubmed
    ..pneumoniae contain d-alanine residues in order to protect this human pathogen against the actions of cationic antimicrobial peptides. ..
  23. Damjanovic M, Kharat A, Eberhardt A, Tomasz A, Vollmer W. The essential tacF gene is responsible for the choline-dependent growth phenotype of Streptococcus pneumoniae. J Bacteriol. 2007;189:7105-11 pubmed
  24. Piñas G, Cortes P, Orio A, Echenique J. Acidic stress induces autolysis by a CSP-independent ComE pathway in Streptococcus pneumoniae. Microbiology. 2008;154:1300-8 pubmed publisher
    ..pneumoniae an alternative condition, in addition to competence and antibiotics, to assure the release of virulence factors, DNA and cell-wall compounds by autolysis, favouring genetic exchange and contributing to its pathogenesis. ..
  25. Charlier P, Buisson G, Dideberg O, Wierenga J, Keck W, Laible G, et al. Crystallization of a genetically engineered water-soluble primary penicillin target enzyme. The high molecular mass PBP2x of Streptococcus pneumoniae. J Mol Biol. 1993;232:1007-9 pubmed
    ..The breakdown of a chromogenic cephalosporin derivative diffused into a crystal reveals clearly that the enzyme is active in the crystalline state. ..
  26. Enright M, Fenoll A, Griffiths D, Spratt B. The three major Spanish clones of penicillin-resistant Streptococcus pneumoniae are the most common clones recovered in recent cases of meningitis in Spain. J Clin Microbiol. 1999;37:3210-6 pubmed
  27. Lange R, Wagner C, de Saizieu A, Flint N, Molnos J, Stieger M, et al. Domain organization and molecular characterization of 13 two-component systems identified by genome sequencing of Streptococcus pneumoniae. Gene. 1999;237:223-34 pubmed
    ..Two response regulator genes could not be inactivated, indicating that they may regulate essential cellular functions. The possibility of using these systems as targets for the development of novel antibacterials will be discussed...
  28. Muramatsu H, Tachikui H, Ushida H, Song X, Qiu Y, Yamamoto S, et al. Molecular cloning and expression of endo-beta-N-acetylglucosaminidase D, which acts on the core structure of complex type asparagine-linked oligosaccharides. J Biochem. 2001;129:923-8 pubmed
    ..pneumoniae and has an LPXTG motif typical of surface-associated proteins of Gram-positive bacteria. It is possible that Endo D is located on the surface of the bacterium and, together with other glycosidases, is involved in virulence...
  29. Noirclerc Savoye M, Le Gou llec A, Morlot C, Dideberg O, Vernet T, Zapun A. In vitro reconstitution of a trimeric complex of DivIB, DivIC and FtsL, and their transient co-localization at the division site in Streptococcus pneumoniae. Mol Microbiol. 2005;55:413-24 pubmed publisher
    ..Taken together, our data suggest that assembly of the trimeric complex DivIB/DivIC/FtsL is regulated during the cell cycle through controlled formation of the DivIC/FtsL heterodimer...
  30. Yamada M, Watanabe T, Miyara T, Baba N, Saito J, Takeuchi Y, et al. Crystal structure of cefditoren complexed with Streptococcus pneumoniae penicillin-binding protein 2X: structural basis for its high antimicrobial activity. Antimicrob Agents Chemother. 2007;51:3902-7 pubmed
    ..These features likely play a role in the high level of activity of cefditoren against S. pneumoniae. ..
  31. Rolo D, Ardanuy C, Fleites A, Mart n R, Li ares J. Diversity of pneumococcal surface protein A (PspA) among prevalent clones in Spain. BMC Microbiol. 2009;9:80 pubmed publisher
    ..PspA clades were associated with genotypes. PspA family 2 and family 1 were dominant among major Spanish pneumococcal clones isolated from patients with invasive disease and nasopharyngeal carriage in children...
  32. Mitchell T, Mendez F, Paton J, Andrew P, Boulnois G. Comparison of pneumolysin genes and proteins from Streptococcus pneumoniae types 1 and 2. Nucleic Acids Res. 1990;18:4010 pubmed
  33. Berry A, Paton J. Sequence heterogeneity of PsaA, a 37-kilodalton putative adhesin essential for virulence of Streptococcus pneumoniae. Infect Immun. 1996;64:5255-62 pubmed
    ..pneumoniae R36A. The deduced amino acid sequences of mature PsaA from the two strains had only 81% homology, with the bulk of the variation occurring in the amino-terminal portion...
  34. Novak R, Henriques B, Charpentier E, Normark S, Tuomanen E. Emergence of vancomycin tolerance in Streptococcus pneumoniae. Nature. 1999;399:590-3 pubmed publisher
    ..We conclude that broad antibiotic tolerance of S. pneumoniae has emerged in the community by a molecular mechanism that eliminates sensitivity to the current cornerstone of therapy, vancomycin...
  35. Brooks Walter A, Briles D, Hollingshead S. The pspC gene of Streptococcus pneumoniae encodes a polymorphic protein, PspC, which elicits cross-reactive antibodies to PspA and provides immunity to pneumococcal bacteremia. Infect Immun. 1999;67:6533-42 pubmed
    ..Mice immunized with PspC were protected against challenge with a strain that expressed PspA but not PspC. The PspA- and PspC-cross-reactive antibodies were directed to the proline-rich domain present in both molecules...
  36. Khandekar S, Gentry D, Van Aller G, Warren P, Xiang H, Silverman C, et al. Identification, substrate specificity, and inhibition of the Streptococcus pneumoniae beta-ketoacyl-acyl carrier protein synthase III (FabH). J Biol Chem. 2001;276:30024-30 pubmed
  37. Dessen A, Mouz N, Gordon E, Hopkins J, Dideberg O. Crystal structure of PBP2x from a highly penicillin-resistant Streptococcus pneumoniae clinical isolate: a mosaic framework containing 83 mutations. J Biol Chem. 2001;276:45106-12 pubmed publisher
    ..Thus, in vivo, an "open" active site could facilitate the recognition of distinct, branched physiological substrates...
  38. Chesnel L, Pernot L, Lemaire D, Champelovier D, Croize J, Dideberg O, et al. The structural modifications induced by the M339F substitution in PBP2x from Streptococcus pneumoniae further decreases the susceptibility to beta-lactams of resistant strains. J Biol Chem. 2003;278:44448-56 pubmed
    ..4 A shows a distortion of the active site and a reorientation of the hydroxyl group of the active site Ser337, which can explain the kinetic effects of the mutations. ..
  39. Kloosterman T, Hendriksen W, Bijlsma J, Bootsma H, van Hijum S, Kok J, et al. Regulation of glutamine and glutamate metabolism by GlnR and GlnA in Streptococcus pneumoniae. J Biol Chem. 2006;281:25097-109 pubmed publisher
    ..pneumoniae. Thus, our results provide a thorough insight into the regulation of glutamine and glutamate metabolism of S. pneumoniae mediated by both GlnR and GlnA...
  40. Bai X, Chen H, Jiang Y, Wen Z, Huang Y, Cheng W, et al. Structure of pneumococcal peptidoglycan hydrolase LytB reveals insights into the bacterial cell wall remodeling and pathogenesis. J Biol Chem. 2014;289:23403-16 pubmed publisher
    ..These findings provided structural insights into the pneumococcal cell wall remodeling and novel hints for the rational design of therapeutic agents against pneumococcal growth and thereby the related diseases. ..
  41. Laible G, Hakenbeck R, Sicard M, Joris B, Ghuysen J. Nucleotide sequences of the pbpX genes encoding the penicillin-binding proteins 2x from Streptococcus pneumoniae R6 and a cefotaxime-resistant mutant, C506. Mol Microbiol. 1989;3:1337-48 pubmed
    ..coli PBP2 and methicillin-resistant Staphylococcus aureus PBP2'. No significant homology was detected with PBP1a or PBP1b of Escherichia coli, or with the low-Mr PBPs...
  42. Zhang Y, Ayalew S, Lacks S. The rnhB gene encoding RNase HII of Streptococcus pneumoniae and evidence of conserved motifs in eucaryotic genes. J Bacteriol. 1997;179:3828-36 pubmed
    ..Partial rnhB gene sequences were detected among expressed sequences of mouse and human cells. From these data, it appears that RNase HII is universally present in living cells. ..
  43. Fraile A, Escriu F, Aranda M, Malpica J, Gibbs A, Garc a Arenal F. A century of tobamovirus evolution in an Australian population of Nicotiana glauca. J Virol. 1997;71:8316-20 pubmed
    ..This phenomenon, called Muller's ratchet, or a "mutational meltdown," probably caused the disappearance of TMV from the niche...
  44. Belanger A, Clague M, Glass J, Leblanc D. Pyruvate oxidase is a determinant of Avery's rough morphology. J Bacteriol. 2004;186:8164-71 pubmed publisher
    ..Thus, the mutated spxB allele did not impact their use of smooth morphology to identify the transforming principle...
  45. Gribun A, Kimber M, Ching R, Sprangers R, Fiebig K, Houry W. The ClpP double ring tetradecameric protease exhibits plastic ring-ring interactions, and the N termini of its subunits form flexible loops that are essential for ClpXP and ClpAP complex formation. J Biol Chem. 2005;280:16185-96 pubmed publisher
    ..Mutation of several amino acid residues in this loop or the truncation of the loop impairs ClpXP and ClpAP complex formation and prevents the coupling between ClpX/ClpA and ClpP activities...
  46. Okwumabua O, Chinnapapakkagari S. Identification of the gene encoding a 38-kilodalton immunogenic and protective antigen of Streptococcus suis. Clin Diagn Lab Immunol. 2005;12:484-90 pubmed
    ..suis capsular types remains to be determined. ..
  47. Tribuddharat C, Polwichai P, Champreeda P, Srifuengfung S. The sequence of pbp2b from penicillin-resistant Streptococcus pneumoniae in Thailand. J Med Assoc Thai. 2010;93 Suppl 5:S16-26 pubmed
    ..Penicillin or cefotaxime resistance in S. pneumoniae in Thailand was due to affinity reduction of PBP2b, similar to changes found in other Asian isolates. ..
  48. Alloing G, Trombe M, Claverys J. The ami locus of the gram-positive bacterium Streptococcus pneumoniae is similar to binding protein-dependent transport operons of gram-negative bacteria. Mol Microbiol. 1990;4:633-44 pubmed
    ..coli. Data are presented which indicate that Ami is indeed a transport system...
  49. Weiser J, Shchepetov M, Chong S. Decoration of lipopolysaccharide with phosphorylcholine: a phase-variable characteristic of Haemophilus influenzae. Infect Immun. 1997;65:943-50 pubmed
    ..In the case of H. influenzae, its primary contribution to pathogenesis does not appear to be antigenic variation to evade host humoral clearance mechanisms...
  50. Kawamura Y, Whiley R, Shu S, Ezaki T, Hardie J. Genetic approaches to the identification of the mitis group within the genus Streptococcus. Microbiology. 1999;145 ( Pt 9):2605-13 pubmed publisher
    ..mitis. We conclude that the partial sequence analysis of the sodA gene could be applied globally as a reliable and easy method for the accurate identification of all species currently within the mitis group...
  51. Hardy G, Caimano M, Yother J. Capsule biosynthesis and basic metabolism in Streptococcus pneumoniae are linked through the cellular phosphoglucomutase. J Bacteriol. 2000;182:1854-63 pubmed
    ..These results demonstrate that most of the PGM activity required for type 3 capsule biosynthesis is derived from the cellular PGM...
  52. Cheng Q, Finkel D, Hostetter M. Novel purification scheme and functions for a C3-binding protein from Streptococcus pneumoniae. Biochemistry. 2000;39:5450-7 pubmed
    ..A similar biochemical approach should enable the identification of other C3-binding proteins in microorganisms able to elude complement-mediated host defense...
  53. Reichmann P, Hakenbeck R. A XerD recombinase with unusual active site motifs in Streptococcus pneumoniae. J Mol Microbiol Biotechnol. 2002;4:101-10 pubmed
    ..The xerD and xerC genes were present in a sample of 20 S. pneumoniae strains whereas the other xer genes appear to be absent in some of the strains and are more closely related to integrases of phage and transposon origin...
  54. Mart n Galiano A, Gorgojo B, Kunin C, de la Campa A. Mefloquine and new related compounds target the F(0) complex of the F(0)F(1) H(+)-ATPase of Streptococcus pneumoniae. Antimicrob Agents Chemother. 2002;46:1680-7 pubmed
    ..These results suggest that the transmembrane helices of the c and a subunits interact and that the mutated residues are important for the structure of the F(0) complex and proton translocation...
  55. Obregón V, Garcia P, López R, Garcia J. VO1, a temperate bacteriophage of the type 19A multiresistant epidemic 8249 strain of Streptococcus pneumoniae: analysis of variability of lytic and putative C5 methyltransferase genes. Microb Drug Resist. 2003;9:7-15 pubmed
    ..The peculiarities of genes like lytA and orfmet in VO1 provide interesting insights on mechanisms of horizontal transfer and lysogenic state co-evolution...
  56. Singh V, Schramm V. Transition-state analysis of S. pneumoniae 5'-methylthioadenosine nucleosidase. J Am Chem Soc. 2007;129:2783-95 pubmed publisher
    ..pneumoniae MTAN. This feature differentiates the S. pneumoniae and E. coli transition states and explains the 10(3)-fold decrease in the catalytic efficiency of S. pneumoniae MTAN relative to that from E. coli...
  57. Romero P, L pez R, Garc a E. Key role of amino acid residues in the dimerization and catalytic activation of the autolysin LytA, an important virulence factor in Streptococcus pneumoniae. J Biol Chem. 2007;282:17729-37 pubmed publisher
    ..The results indicate that dimerization and conversion are different processes, but they do not answer the questions of whether conversion can only be achieved after a dimer formation step...
  58. Kim D, San B, Moh S, Park H, Kim D, Lee S, et al. Structural basis for the substrate specificity of PepA from Streptococcus pneumoniae, a dodecameric tetrahedral protease. Biochem Biophys Res Commun. 2010;391:431-6 pubmed publisher
  59. Jiang Y, Yu W, Zhang J, Frolet C, Di Guilmi A, Zhou C, et al. Structural basis for the substrate specificity of a novel β-N-acetylhexosaminidase StrH protein from Streptococcus pneumoniae R6. J Biol Chem. 2011;286:43004-12 pubmed publisher
    ..The putative surface-exposed N-acetylhexosaminidase StrH/Spr0057 from Streptococcus pneumoniae R6 was proved to contribute to the virulence by removal of β(1,2)-linked NAG on host defense molecules ..
  60. Soares da Costa T, Desbois S, Dogovski C, Gorman M, Ketaren N, Paxman J, et al. Structural Determinants Defining the Allosteric Inhibition of an Essential Antibiotic Target. Structure. 2016;24:1282-1291 pubmed publisher
    ..This study has unveiled the molecular determinants defining lysine-mediated allosteric inhibition of bacterial DHDPS. ..
  61. Hui F, Morrison D. Identification of a purC gene from Streptococcus pneumoniae. J Bacteriol. 1993;175:6364-7 pubmed
    ..Function of the S. pneumoniae gene product was demonstrated by complementation of E. coli purC mutations. ..
  62. Whatmore A, Dowson C. The autolysin-encoding gene (lytA) of Streptococcus pneumoniae displays restricted allelic variation despite localized recombination events with genes of pneumococcal bacteriophage encoding cell wall lytic enzymes. Infect Immun. 1999;67:4551-6 pubmed
    ..The implications of these findings for understanding the evolution of lytA and the potential utility of LytA as a vaccine target are discussed...
  63. Almir n Roig E, Mulholland F, Gasson M, Griffin A. The complete cps gene cluster from Streptococcus thermophilus NCFB 2393 involved in the biosynthesis of a new exopolysaccharide. Microbiology. 2000;146 ( Pt 11):2793-802 pubmed publisher
    ..Analysis of the expression of the cpsE gene in Escherichia coli demonstrated that it encodes a glucose-1-phosphate transferase; the enzyme which catalyses the first step in EPS biosynthesis in S. thermophilus NCFB 2393...
  64. Iannelli F, Oggioni M, Pozzi G. Allelic variation in the highly polymorphic locus pspC of Streptococcus pneumoniae. Gene. 2002;284:63-71 pubmed
    ..This major difference in the anchor region was also observed in the adjacent proline-rich regions which differed considerably in size and composition...
  65. Prudhomme M, Martin B, Mejean V, Claverys J. Nucleotide sequence of the Streptococcus pneumoniae hexB mismatch repair gene: homology of HexB to MutL of Salmonella typhimurium and to PMS1 of Saccharomyces cerevisiae. J Bacteriol. 1989;171:5332-8 pubmed
    ..This finding also suggests that the Hex, Mut, and PMS systems evolved from a common ancestor and that functionally similar mismatch repair systems could be widespread among procaryotic as well as eucaryotic organisms...
  66. Lacks S, Mannarelli B, Springhorn S, Greenberg B. Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intercellular cassette mechanism. Cell. 1986;46:993-1000 pubmed
    ..Thus, the complementary restriction systems are found on nonhomologous and mutually exclusive cassettes that can be inserted into a particular point in the chromosome of S. pneumoniae on the basis of neighboring homology...
  67. Claverys J, M jean V, Gasc A, Galibert F, Sicard A. Base specificity of mismatch repair in Streptococcus pneumoniae. Nucleic Acids Res. 1981;9:2267-80 pubmed
    ..pneumoniae is directed against mismatched pyrimidines. DNA sequence analysis also reveals that short deletions (33 or 34 bases long) behave as very high efficiency markers, confirming that deletions are not recognized by the hex system...
  68. Lacks S, Dunn J, Greenberg B. Identification of base mismatches recognized by the heteroduplex-DNA-repair system of Streptococcus pneumoniae. Cell. 1982;31:327-36 pubmed
  69. Mu oz R, de la Campa A. ParC subunit of DNA topoisomerase IV of Streptococcus pneumoniae is a primary target of fluoroquinolones and cooperates with DNA gyrase A subunit in forming resistance phenotype. Antimicrob Agents Chemother. 1996;40:2252-7 pubmed
    ..coli GyrA subunit. Transformation experiments suggest that ParC is the primary target of ciprofloxacin. Mutation in parC appears to be a prerequisite before mutations in gyrA can influence resistance levels...
  70. Poyart C, Quesne G, Coulon S, Berche P, Trieu Cuot P. Identification of streptococci to species level by sequencing the gene encoding the manganese-dependent superoxide dismutase. J Clin Microbiol. 1998;36:41-7 pubmed
    ..We propose this method for the characterization of strains that cannot be assigned to a species on the basis of their conventional phenotypic reactions...
  71. Garc a P, Paz Gonz lez M, Garc a E, Garc a J, L pez R. The molecular characterization of the first autolytic lysozyme of Streptococcus pneumoniae reveals evolutionary mobile domains. Mol Microbiol. 1999;33:128-38 pubmed
    ..This work illustrates the natural versatility exhibited by the pneumococcal genes coding for choline-binding proteins to fuse separated catalytic and substrate-binding domains and create new and functional mature proteins...
  72. Gherardi G, Whitney C, Facklam R, Beall B. Major related sets of antibiotic-resistant Pneumococci in the United States as determined by pulsed-field gel electrophoresis and pbp1a-pbp2b-pbp2x-dhf restriction profiles. J Infect Dis. 2000;181:216-29 pubmed publisher
    ..Divergent capsular types among isolates with identical PBP gene-dhf profiles and related PFGE types indicated several instances of capsular serotype switching...
  73. Gordon E, Mouz N, Du e E, Dideberg O. The crystal structure of the penicillin-binding protein 2x from Streptococcus pneumoniae and its acyl-enzyme form: implication in drug resistance. J Mol Biol. 2000;299:477-85 pubmed publisher
    ..The structure highlights a few amino acid residues, namely Thr338, Thr550 and Gln552, which are directly related to the resistance phenomenon...
  74. Chalker A, Ingraham K, Lunsford R, Bryant A, Bryant J, Wallis N, et al. The bacA gene, which determines bacitracin susceptibility in Streptococcus pneumoniae and Staphylococcus aureus, is also required for virulence. Microbiology. 2000;146 ( Pt 7):1547-53 pubmed publisher
    ..These observations are consistent with the previously proposed role of BacA protein as undecaprenol kinase...
  75. Reichmann P, Hakenbeck R. Allelic variation in a peptide-inducible two-component system of Streptococcus pneumoniae. FEMS Microbiol Lett. 2000;190:231-6 pubmed
    ..A direct repeat element upstream of the spiR1 promoter and another three potential transcription start sites within the bacteriocin cluster indicate that SpiP functions as an inducing peptide for bacteriocin synthesis in S. pneumoniae...
  76. Margolis P, Hackbarth C, Lopez S, Maniar M, Wang W, Yuan Z, et al. Resistance of Streptococcus pneumoniae to deformylase inhibitors is due to mutations in defB. Antimicrob Agents Chemother. 2001;45:2432-5 pubmed
    ..pneumoniae ATCC 49619 harbor mutations in defB but not in fmt. Reintroduction of the mutated defB gene into wild-type S. pneumoniae R6x recreates the resistance phenotype. The altered enzyme displays decreased sensitivity to actinonin...
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    ..In contrast, most atypical isolates formed long chains of cells that did not disperse after addition of purified LytB, suggesting that in these strains chains were produced through mechanisms unrelated to LytB...
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    ..However, in the presence of 1 mM EDTA, the fraction of target cells lysed directly by CbpD increased dramatically, indicating that divalent cations are involved in the regulation of fratricide under natural conditions...
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    ..These results suggest that a FlaB-PspA fusion protein alone could be used as an anti-pneumococcal mucosal vaccine or as an effective partner protein for multivalent capsular polysaccharide conjugate vaccines...
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    ..These results suggest that DNA topoisomerase IV is an important target for fluoroquinolones in S. pneumoniae and establish this organism as a useful gram-positive system for resistance studies...
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    ..None of the pbp3 genes had a mosaic structure, but in three cases there was evidence for a site-specific recombination event within a BOX element immediately downstream of pbp3...
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    ..oralis, suggesting that these particular organisms may be a potential source of DNA in recombination events generating the mosaic structures commonly reported in genes of S. pneumoniae that are under strong selective pressure...
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    ..This study can also contribute to solving diagnostic problems and to understanding the evolution and pathogenic potential of species of the Streptococcus mitis group...
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    ..One of these new structures resembles the S.agalactiae enzyme conformation, and provides evidence of a uniform mechanistic/dynamic behavior of this protein across different bacteria...
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    ..Finally, a D39 Deltarel(Spn) mutant was severely attenuated and displayed a significantly altered course of disease progression in a mouse model of infection, which was restored to normal by an ectopic copy of rel(+)(Spn)...
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    ..Our study also demonstrates that ciaH alleles overstimulating CiaR regulon expression are present in clinical isolates of S. pneumoniae...
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    ..The method described in this study provides a means for easy recovery of sod genes and the construction of sod mutants of various Gram-positive pathogens...
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    ..These results suggest that GAPDHs of various plaque-forming streptococci may be involved in their attachment to P. gingivalis fimbriae and that they may contribute to P. gingivalis colonization...
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    ..The multiple binding capacity of the SDH in conjunction with its GAPDH activity may play a role in the colonization, internalization, and the subsequent proliferation of group A streptococci...
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    ..Taken together, these results lead us to propose that Adc is an ABC-type Zn permease, the first such protein complex identified in any organism, and that Psa is an ABC-type Mn permease complex...