Streptococcus mutans UA159

Summary

Alias: Streptococcus mutans str. UA159

Top Publications

  1. Wen Z, Burne R. LuxS-mediated signaling in Streptococcus mutans is involved in regulation of acid and oxidative stress tolerance and biofilm formation. J Bacteriol. 2004;186:2682-91 pubmed
    ..This study demonstrates that luxS-dependent signaling plays critical roles in modulating key virulence properties of S. mutans. ..
  2. Duque C, Stipp R, Wang B, Smith D, Hofling J, Kuramitsu H, et al. Downregulation of GbpB, a component of the VicRK regulon, affects biofilm formation and cell surface characteristics of Streptococcus mutans. Infect Immun. 2011;79:786-96 pubmed publisher
    ..These data provide the first experimental evidence for GbpB participation in sucrose-dependent biofilm formation and in cell surface properties. ..
  3. Shiroza T, Kuramitsu H. Sequence analysis of the Streptococcus mutans fructosyltransferase gene and flanking regions. J Bacteriol. 1988;170:810-6 pubmed
    ..In addition, ORF 3 is transcribed from the ftf DNA coding strand. Partial sequencing of ORF 4 suggests that its gene product may be an extracellular protein. ..
  4. Burne R, Wen Z, Chen Y, Penders J. Regulation of expression of the fructan hydrolase gene of Streptococcus mutans GS-5 by induction and carbon catabolite repression. J Bacteriol. 1999;181:2863-71 pubmed
  5. Ahn S, Lemos J, Burne R. Role of HtrA in growth and competence of Streptococcus mutans UA159. J Bacteriol. 2005;187:3028-38 pubmed
  6. Chong P, Drake L, Biswas I. Modulation of covR expression in Streptococcus mutans UA159. J Bacteriol. 2008;190:4478-88 pubmed publisher
    ..A detailed analysis of the regulation of CovR may lead to a better understanding of the pathogenesis of S. mutans, as well as providing further insight into the prevention of dental caries. ..
  7. Seaton K, Ahn S, Sagstetter A, Burne R. A transcriptional regulator and ABC transporters link stress tolerance, (p)ppGpp, and genetic competence in Streptococcus mutans. J Bacteriol. 2011;193:862-74 pubmed publisher
  8. Sato Y, Poy F, Jacobson G, Kuramitsu H. Characterization and sequence analysis of the scrA gene encoding enzyme IIScr of the Streptococcus mutans phosphoenolpyruvate-dependent sucrose phosphotransferase system. J Bacteriol. 1989;171:263-71 pubmed
    ..mutans protein was also homologous with the Salmonella typhimurium enzyme IIIGlc and the 162-amino-acid C terminus of E. coli enzyme IIBgl. These results suggest that the sucrose PTS system of S. mutans is enzyme III independent. ..
  9. Ueda S, Shiroza T, Kuramitsu H. Sequence analysis of the gtfC gene from Streptococcus mutans GS-5. Gene. 1988;69:101-9 pubmed
    ..The possible evolution of the gtfC gene in S. mutans GS-5 is also discussed. ..

More Information

Publications117 found, 100 shown here

  1. Shiroza T, Ueda S, Kuramitsu H. Sequence analysis of the gtfB gene from Streptococcus mutans. J Bacteriol. 1987;169:4263-70 pubmed
    ..Nucleotide sequencing of the region immediately downstream from the gtfB gene revealed the presence of a putative gene coding for an extracellular protein. This open reading frame is partially homologous to the gtfB gene. ..
  2. Yamashita Y, Takehara T, Kuramitsu H. Molecular characterization of a STreptococcus mutans mutant altered in environmental stress responses. J Bacteriol. 1993;175:6220-8 pubmed
    ..These results suggest that the S. mutans homolog of diacylglycerol kinase is important for adaptation of the organism to several environmental stress signals. ..
  3. Sato Y, Yamamoto Y, Kizaki H. Cloning and sequence analysis of the gbpC gene encoding a novel glucan-binding protein of Streptococcus mutans. Infect Immun. 1997;65:668-75 pubmed
    ..Extract from an E. coli clone of the gene exhibited glucan-binding activity. Therefore, the gene encoded a novel glucan-binding protein. ..
  4. Simpson C, Russell R. Identification of a homolog of CcpA catabolite repressor protein in Streptococcus mutans. Infect Immun. 1998;66:2085-92 pubmed
    ..These results suggest that while RegM can affect catabolite repression in S. mutans, it does not conform to the model proposed for CcpA in Bacillus subtilis. ..
  5. Gutierrez J, Crowley P, Cvitkovitch D, Brady L, Hamilton I, Hillman J, et al. Streptococcus mutans ffh, a gene encoding a homologue of the 54 kDa subunit of the signal recognition particle, is involved in resistance to acid stress. Microbiology. 1999;145 ( Pt 2):357-66 pubmed
    ..These findings suggest that Ffh may be involved in the maintenance of a functional membrane protein composition during adaptation of S. mutans to changing environmental conditions. ..
  6. Sato Y, Yamamoto Y, Kizaki H. Construction of region-specific partial duplication mutants (merodiploid mutants) to identify the regulatory gene for the glucan-binding protein C gene in vivo in Streptococcus mutans. FEMS Microbiol Lett. 2000;186:187-91 pubmed
    ..The 690-bp gene responsible for the ddag(-) phenotype was identified within a 60-kb region by observing ddag (positive or negative) phenotypes of successively constructed specific duplication mutants. ..
  7. Cote C, Cvitkovitch D, Bleiweis A, Honeyman A. A novel beta-glucoside-specific PTS locus from Streptococcus mutans that is not inhibited by glucose. Microbiology. 2000;146 ( Pt 7):1555-63 pubmed
    ..When a plasmid containing the 8 kb beta-glucoside-specific regulon was transformed into E. coli CC118, the transformed strain was able to break down the beta-glucoside arbutin. ..
  8. Wen Z, Browngardt C, Burne R. Characterization of two operons that encode components of fructose-specific enzyme II of the sugar:phosphotransferase system of Streptococcus mutans. FEMS Microbiol Lett. 2001;205:337-42 pubmed
    ..Xylitol toxicity assays indicated that the inducible fructose permease was responsible for xylitol transport. ..
  9. Wen Z, Burne R. Functional genomics approach to identifying genes required for biofilm development by Streptococcus mutans. Appl Environ Microbiol. 2002;68:1196-203 pubmed
    ..From these data, we conclude that CcpA and BrpA may regulate genes that are required for stable biofilm formation by S. mutans. ..
  10. Wen Z, Suntharaligham P, Cvitkovitch D, Burne R. Trigger factor in Streptococcus mutans is involved in stress tolerance, competence development, and biofilm formation. Infect Immun. 2005;73:219-25 pubmed
    ..These results suggest that RopA is a key regulator of acid and oxidative stress tolerance, genetic competence, and biofilm formation, all critical virulence properties of S. mutans. ..
  11. Wen Z, Baker H, Burne R. Influence of BrpA on critical virulence attributes of Streptococcus mutans. J Bacteriol. 2006;188:2983-92 pubmed
    ..mutans, which are key virulence attributes of the organism. ..
  12. Biswas I, Drake L, Biswas S. Regulation of gbpC expression in Streptococcus mutans. J Bacteriol. 2007;189:6521-31 pubmed
    ..In summary, our results indicate that various stress conditions modulate the expression of gbpC and that CovR negatively regulates the expression of the gbpC gene by directly binding to the promoter region. ..
  13. Ahn S, Burne R. Effects of oxygen on biofilm formation and the AtlA autolysin of Streptococcus mutans. J Bacteriol. 2007;189:6293-302 pubmed
    ..This study illustrates the central role of AtlA and VicK in orchestrating growth on surfaces and envelope biogenesis in response to redox conditions. ..
  14. Chattoraj P, Banerjee A, Biswas S, Biswas I. ClpP of Streptococcus mutans differentially regulates expression of genomic islands, mutacin production, and antibiotic tolerance. J Bacteriol. 2010;192:1312-23 pubmed publisher
    ..The results presented here indicate that ClpP-mediated proteolysis plays an important global role in the regulation of several important traits in this pathogen. ..
  15. Chong P, Chattoraj P, Biswas I. Activation of the SMU.1882 transcription by CovR in Streptococcus mutans. PLoS ONE. 2010;5:e15528 pubmed publisher
    ..mutans RpoD, E. coli RNA polymerase, and CovR did not activate transcription from this promoter. Thus, we speculate that in vivo, CovR may interfere with the binding of a repressor or requires a cofactor...
  16. Hung D, Downey J, Ayala E, Kreth J, Mair R, Senadheera D, et al. Characterization of DNA binding sites of the ComE response regulator from Streptococcus mutans. J Bacteriol. 2011;193:3642-52 pubmed publisher
    ..Based on the mutational analysis and DNase I footprinting analysis, we propose a consensus ComE binding site, TCBTAAAYSGT. ..
  17. Ahn S, Qu M, Roberts E, Burne R, Rice K. Identification of the Streptococcus mutans LytST two-component regulon reveals its contribution to oxidative stress tolerance. BMC Microbiol. 2012;12:187 pubmed publisher
    ..In this study, we sought to determine if LytST was involved in regulating lrgAB expression in response to glucose and oxygenation in S. mutans...
  18. Cvitkovitch D, Gutierrez J, Bleiweis A. Role of the citrate pathway in glutamate biosynthesis by Streptococcus mutans. J Bacteriol. 1997;179:650-5 pubmed
    ..Indeed, S. mutans JH1005 was shown to assimilate ammonia as a sole source of nitrogen in minimal medium devoid of organic nitrogen sources. ..
  19. Li Y, Lau P, Lee J, Ellen R, Cvitkovitch D. Natural genetic transformation of Streptococcus mutans growing in biofilms. J Bacteriol. 2001;183:897-908 pubmed
    ..This work demonstrated that a peptide pheromone system controls genetic competence in S. mutans and that the system functions optimally when the cells are living in actively growing biofilms. ..
  20. Griswold A, Chen Y, Burne R. Analysis of an agmatine deiminase gene cluster in Streptococcus mutans UA159. J Bacteriol. 2004;186:1902-4 pubmed
    ..encoding enzymes of the agmatine deiminase system (AgDS) has been identified in the cariogenic bacterium Streptococcus mutans UA159. The AgDS is regulated by agmatine induction and carbohydrate catabolite repression...
  21. Browngardt C, Wen Z, Burne R. RegM is required for optimal fructosyltransferase and glucosyltransferase gene expression in Streptococcus mutans. FEMS Microbiol Lett. 2004;240:75-9 pubmed
    ..These findings reinforce the importance of pH and carbohydrate availability for expression of two primary virulence attributes of S. mutans and reveal a critical role for RegM in regulation of expression of both gtfBC and ftf. ..
  22. Shibata Y, Kawada M, Nakano Y, Toyoshima K, Yamashita Y. Identification and characterization of an autolysin-encoding gene of Streptococcus mutans. Infect Immun. 2005;73:3512-20 pubmed
    ..mutans. These results indicate that the catalytic domain of AtlA is located at the C terminus, suggesting that further characterization of this domain may provide a means to control cariogenic dental plaque formation. ..
  23. Syvitski R, Tian X, Sampara K, Salman A, Lee S, Jakeman D, et al. Structure-activity analysis of quorum-sensing signaling peptides from Streptococcus mutans. J Bacteriol. 2007;189:1441-50 pubmed
    ..This study is the first study to recognize the importance of the signaling peptide C-terminal residues in streptococcal quorum sensing. ..
  24. Sztajer H, Lemme A, Vilchez R, Schulz S, Geffers R, Yip C, et al. Autoinducer-2-regulated genes in Streptococcus mutans UA159 and global metabolic effect of the luxS mutation. J Bacteriol. 2008;190:401-15 pubmed
    ..signaling role of AI-2, we carried out a global transcriptome analysis of a luxS null mutant culture of Streptococcus mutans UA159, an important cariogenic bacterium and a crucial component of the dental plaque biofilm community, in ..
  25. Abranches J, Nascimento M, Zeng L, Browngardt C, Wen Z, Rivera M, et al. CcpA regulates central metabolism and virulence gene expression in Streptococcus mutans. J Bacteriol. 2008;190:2340-9 pubmed publisher
    ..5, and could acidify the environment more rapidly and to a greater extent than the parental strain. Thus, CcpA directly modulates the pathogenic potential of S. mutans through global control of gene expression. ..
  26. Dunning D, McCall L, Powell W, Arscott W, McConocha E, McClurg C, et al. SloR modulation of the Streptococcus mutans acid tolerance response involves the GcrR response regulator as an essential intermediary. Microbiology. 2008;154:1132-43 pubmed publisher
    ..Taking these results together, we propose that SloR-Mn modulates S. mutans gcrR expression as part of a general stress response, and that GcrR acts downstream of SloR to control the ATR. ..
  27. Liu C, Li D, Liang Y, Li L, Su X. Ring-opening mechanism revealed by crystal structures of NagB and its ES intermediate complex. J Mol Biol. 2008;379:73-81 pubmed publisher
  28. Niu G, Okinaga T, Zhu L, Banas J, Qi F, Merritt J. Characterization of irvR, a novel regulator of the irvA-dependent pathway required for genetic competence and dextran-dependent aggregation in Streptococcus mutans. J Bacteriol. 2008;190:7268-74 pubmed publisher
    ..These data indicate that the repression of irvA is critically dependent upon irvR and that irvA repression is essential for the development of genetic competence and the proper control of DDAG in S. mutans. ..
  29. Wu C, Ayala E, Downey J, Merritt J, Goodman S, Qi F. Regulation of ciaXRH operon expression and identification of the CiaR regulon in Streptococcus mutans. J Bacteriol. 2010;192:4669-79 pubmed publisher
    ..Taken together, we conclude that overexpressed CiaR, as a result of either ciaH deletion or forced expression from a constitutive promoter, is a mediator in the CiaH-regulated phenotypes. ..
  30. Tian X, Dong G, Liu T, Gomez Z, Wahl A, Hols P, et al. MecA protein acts as a negative regulator of genetic competence in Streptococcus mutans. J Bacteriol. 2013;195:5196-206 pubmed publisher
    ..mutans. Together, our data support the notion that MecA mediates the formation of a ternary SigX-MecA-ClpC complex that sequesters SigX and thereby negatively regulates genetic competence in S. mutans. ..
  31. Shah D, Russell R. A novel glucan-binding protein with lipase activity from the oral pathogen Streptococcus mutans. Microbiology. 2004;150:1947-56 pubmed publisher
    ..sanguinis, but had no effect on LTA from S. mutans. These results raise the intriguing possibility that GbpD may be involved in direct interspecies competition within the plaque biofilm...
  32. Sato Y, Yamamoto Y, Kizaki H, Kuramitsu H. Isolation and sequence analysis of the pmi gene encoding phosphomannose isomerase of Streptococcus mutans. FEMS Microbiol Lett. 1993;114:61-6 pubmed
    ..mutans also drastically reduced phosphomannose isomerase activity and the ability of the organism to utilize mannose as a sole carbon source. These results suggest that the S. mutans pmi gene constitutes an operon with the scrK gene. ..
  33. Crowley P, Gutierrez J, Hillman J, Bleiweis A. Genetic and physiologic analysis of a formyl-tetrahydrofolate synthetase mutant of Streptococcus mutans. J Bacteriol. 1997;179:1563-72 pubmed
    ..This represents the first characterization of Fhs in Streptococcus...
  34. Cobessi D, T te Favier F, Marchal S, Branlant G, Aubry A. Structural and biochemical investigations of the catalytic mechanism of an NADP-dependent aldehyde dehydrogenase from Streptococcus mutans. J Mol Biol. 2000;300:141-52 pubmed publisher
    ..It is now positioned in a hydrophobic environment. This supports the pK(a) assignment of 7.6 to Glu250 as recently proposed from enzymatic studies...
  35. Terao Y, Isoda R, Murakami J, Hamada S, Kawabata S. Molecular and biological characterization of gtf regulation-associated genes in Streptococcus mutans. Oral Microbiol Immunol. 2009;24:211-7 pubmed publisher
    ..These findings suggest that orf1 and orf2 are associated with positive regulation of the gtfB and gtfC genes. ..
  36. Perry J, Cvitkovitch D, L vesque C. Cell death in Streptococcus mutans biofilms: a link between CSP and extracellular DNA. FEMS Microbiol Lett. 2009;299:261-6 pubmed publisher
    ..mutans biofilm formation through the release of chromosomal DNA into the extracellular matrix, providing a long sought-after mechanistic explanation for the role of CSP in S. mutans biofilm formation...
  37. Zhao W, Zhan X, Gao X, Liu X, Zhang Y, Lin J, et al. Preliminary X-ray crystallographic analysis of SMU.2055 protein from the caries pathogen Streptococcus mutans. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010;66:530-3 pubmed publisher
    ..The crystals belong to the orthorhombic space group C222(1), with unit-cell parameters a = 92.0, b = 95.0, c = 192.2 A. The asymmetric unit contained four molecules, with a solvent content of 57.1%. ..
  38. Larson M, Rajashankar K, Crowley P, Kelly C, Mitchell T, Brady L, et al. Crystal structure of the C-terminal region of Streptococcus mutans antigen I/II and characterization of salivary agglutinin adherence domains. J Biol Chem. 2011;286:21657-66 pubmed publisher
    ..Finally, electron microscopy images confirmed the elongated structure of AgI/II and enabled building a composite tertiary model that encompasses its two distinct binding regions. ..
  39. Kajfasz J, Abranches J, LEMOS J. Transcriptome analysis reveals that ClpXP proteolysis controls key virulence properties of Streptococcus mutans. Microbiology. 2011;157:2880-90 pubmed publisher
    ..Collectively, our results reveal the broad scope of ClpXP regulation in S. mutans homeostasis and identify several virulence-related traits that are influenced by ClpXP proteolysis. ..
  40. Kim T, Im D, Jung H, Kwon S, Heo Y. Purification, crystallization and preliminary X-ray diffraction analysis of enoyl-acyl carrier protein reductase (FabK) from Streptococcus mutans strain UA159. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012;68:292-4 pubmed publisher
    ..The crystal belonged to space group P6(2), with unit-cell parameters a = b = 105.79, c = 44.15 Å. The asymmetric unit contained one molecule, with a corresponding V(M) of 2.05 Å(3) Da(-1) and a solvent content of 39.9%. ..
  41. He Z, Liang J, Tang Z, Ma R, Peng H, Huang Z. Role of the luxS gene in initial biofilm formation by Streptococcus mutans. J Mol Microbiol Biotechnol. 2015;25:60-8 pubmed
    ..Most of the genes examined were downregulated in the biofilms formed by the luxS mutant strain, except the gtfB gene. QS luxS gene can affect the initial biofilm formation by S. mutans. ..
  42. Sadeghinejad L, Cvitkovitch D, Siqueira W, Merritt J, Santerre J, Finer Y. Mechanistic, genomic and proteomic study on the effects of BisGMA-derived biodegradation product on cariogenic bacteria. Dent Mater. 2017;33:175-190 pubmed publisher
    ..e. gtfB, gtfC, gbpB, comC, comD, comE and atpH in Streptococcus mutans UA159. Possible mechanisms for bacterial response to BisHPPP were explored using gene knock-out and associated ..
  43. Duncan M, Hillman J. DNA sequence and in vitro mutagenesis of the gene encoding the fructose-1,6-diphosphate-dependent L-(+)-lactate dehydrogenase of Streptococcus mutans. Infect Immun. 1991;59:3930-4 pubmed
    ..mutans was inserted at the deletion site as a marker for selection. In addition, evidence from Southern hybridization showed that S. mutans JH1000 contained a single copy of the lactate dehydrogenase gene. ..
  44. Yamamoto Y, Sato Y, Takahashi Abbe S, Takahashi N, Kizaki H. Characterization of the Streptococcus mutans pyruvate formate-lyase (PFL)-activating enzyme gene by complementary reconstitution of the In vitro PFL-reactivating system. Infect Immun. 2000;68:4773-7 pubmed
    ..The S. mutans act gene encoded the sole enzyme able to activate the PFL protein in this organism...
  45. Ozaki K, Shibata Y, Yamashita Y, Nakano Y, Tsuda H, Koga T. A novel mechanism for glucose side-chain formation in rhamnose-glucose polysaccharide synthesis. FEBS Lett. 2002;532:159-63 pubmed
    ..coli: one gene encodes a glucosyltransferase, and the other gene probably controls the frequency of branching. This is the first report to identify a gene that is involved in regulation of branching frequency in polysaccharide synthesis...
  46. Haswell J, Pruitt B, Cornacchione L, Coe C, Smith E, Spatafora G. Characterization of the functional domains of the SloR metalloregulatory protein in Streptococcus mutans. J Bacteriol. 2013;195:126-34 pubmed publisher
    ..Given these results, we describe the functional domains of the S. mutans SloR protein and propose that the hyperactive mutant could serve as a target for rational drug design aimed at repressing SloR-mediated virulence gene expression. ..
  47. Fujishima K, Kawada Matsuo M, Oogai Y, Tokuda M, Torii M, Komatsuzawa H. dpr and sod in Streptococcus mutans are involved in coexistence with S. sanguinis, and PerR is associated with resistance to H2O2. Appl Environ Microbiol. 2013;79:1436-43 pubmed publisher
    ..Our results suggest that dpr and sod in S. mutans are involved in coexistence with S. sanguinis, and PerR is associated with resistance to H(2)O(2) in regulating the expression of Dpr. ..
  48. Ferretti J, Russell R, Dao M. Sequence analysis of the wall-associated protein precursor of Streptococcus mutans antigen A. Mol Microbiol. 1989;3:469-78 pubmed
    ..Thus, the wall-associated protein A is a precursor of the 29,000 Mr antigen A...
  49. Martin M, Byers B, Olson M, Salin M, Arceneaux J, Tolbert C. A Streptococcus mutans superoxide dismutase that is active with either manganese or iron as a cofactor. J Biol Chem. 1986;261:9361-7 pubmed
    ..Such "cambialistic" enzymes (those capable of making a cofactor substitution) may represent a previously unrecognized family of superoxide dismutases. ..
  50. Chen P, Novak J, Qi F, Caufield P. Diacylglycerol kinase is involved in regulation of expression of the lantibiotic mutacin II of Streptococcus mutans. J Bacteriol. 1998;180:167-70 pubmed
    ..These data indicated that the full-length dgk gene product along with the mutacin biosynthetic operon are required for the production of the mutacin II lantibiotic. ..
  51. Germond J, Delley M, D Amico N, Vincent S. Heterologous expression and characterization of the exopolysaccharide from Streptococcus thermophilus Sfi39. Eur J Biochem. 2001;268:5149-56 pubmed
  52. Mattos Graner R, Jin S, King W, Chen T, Smith D, Duncan M. Cloning of the Streptococcus mutans gene encoding glucan binding protein B and analysis of genetic diversity and protein production in clinical isolates. Infect Immun. 2001;69:6931-41 pubmed publisher
  53. Okamoto Shibayama K, Sato Y, Yamamoto Y, Ohta K, Kizaki H. Identification of a glucan-binding protein C gene homologue in Streptococcus macacae. Oral Microbiol Immunol. 2006;21:32-41 pubmed publisher
    ..mutans GbpC protein. In addition, Southern hybridization analysis using the S. macacae gbpC homologue as a probe showed a distribution of gbpC homologues throughout the mutans streptococci...
  54. Nascimento M, Lemos J, Abranches J, Lin V, Burne R. Role of RelA of Streptococcus mutans in global control of gene expression. J Bacteriol. 2008;190:28-36 pubmed publisher
    The production of (p)ppGpp by Streptococcus mutans UA159 is catalyzed by three gene products: RelA, RelP, and RelQ. Here, we investigate the role of the RelA (Rel) homologue of S...
  55. Aduse Opoku J, Tao L, Ferretti J, Russell R. Biochemical and genetic analysis of Streptococcus mutans alpha-galactosidase. J Gen Microbiol. 1991;137:757-64 pubmed
    ..Inactivation of the aga gene in S. mutans resulted in loss of all alpha-galactosidase activity and abolished the ability to ferment melibiose; alpha-glucosidase activity was also lost, due to an indirect effect on the dexB gene. ..
  56. Kelly C, Evans P, Ma J, Bergmeier L, Taylor W, Brady L, et al. Sequencing and characterization of the 185 kDa cell surface antigen of Streptococcus mutans. Arch Oral Biol. 1990;35 Suppl:33S-38S pubmed
    ..N-terminal sequencing of purified antigens I and II components indicated that antigen I extends from the amino-terminus of the intact Mr 185,000 surface antigen while antigen II extends from residue 996. ..
  57. Boyd D, Cvitkovitch D, Hamilton I. Sequence, expression, and function of the gene for the nonphosphorylating, NADP-dependent glyceraldehyde-3-phosphate dehydrogenase of Streptococcus mutans. J Bacteriol. 1995;177:2622-7 pubmed
    ..mutans NG5 lacks both glucose-6-phosphate dehydrogenase and NADH:NADP oxidoreductase activities, the NADP-dependent glyceraldehyde-3-phosphate dehydrogenase is important as a means of generating NADPH for biosynthetic reactions...
  58. Hussain H, Branny P, Allan E. A eukaryotic-type serine/threonine protein kinase is required for biofilm formation, genetic competence, and acid resistance in Streptococcus mutans. J Bacteriol. 2006;188:1628-32 pubmed publisher
    ..Mutation of the gene encoding the STPK produced defects in biofilm formation, genetic competence, and acid resistance, determinants important in caries pathogenesis...
  59. Old L, Russell R. Distribution and activity of IS elements in Streptococcus mutans. FEMS Microbiol Lett. 2008;287:199-204 pubmed publisher
    ..ISSmu1 was demonstrated to be capable of transposition at a low frequency but no transposition of ISSmu2 was observed...
  60. Li G, Liu X, Nan J, Brostromer E, Li L, Su X. Open-closed conformational change revealed by the crystal structures of 3-keto-L-gulonate 6-phosphate decarboxylase from Streptococcus mutans. Biochem Biophys Res Commun. 2009;381:429-33 pubmed publisher
    ..The open-closed conformational changes of the active site, through the movements of the alpha-helix lid and the arginine residues are important for substrate binding and catalysis. ..
  61. Busuioc M, Mackiewicz K, Buttaro B, Piggot P. Role of intracellular polysaccharide in persistence of Streptococcus mutans. J Bacteriol. 2009;191:7315-22 pubmed publisher
    ..IPS was not needed for persistence when sucrose was the carbon source or when mucin was present...
  62. Kang K, Lee J, Yoo M, Jin I. The influence of HtrA expression on the growth of Streptococcus mutans during acid stress. Mol Cells. 2010;29:297-304 pubmed publisher
    ..Further, similar results were obtained for S. mutans UA159. Thus, htrA in S. mutans K7, as well as S. mutans UA159, can be concluded to play an important role during acid stress...
  63. Cao Y, Li G, Wang K, Zhang H, Li L. Crystallization and preliminary X-ray analysis of argininosuccinate lyase from Streptococcus mutans. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011;67:682-4 pubmed publisher
    ..Crystals suitable for X-ray analysis were obtained and X-ray diffraction data were collected to a resolution of 2.5?Å. The crystals belonged to space group R3, with unit-cell parameters a = b = 254.5, c = 78.3?Å. ..
  64. Tao L, Chattoraj P, Biswas I. CtsR regulation in mcsAB-deficient Gram-positive bacteria. J Bacteriol. 2012;194:1361-8 pubmed publisher
    ..We speculate that CtsR is regulated posttranslationally by a different mechanism in S. mutans and possibly in other streptococci...
  65. Bitoun J, Liao S, Xie G, Beatty W, Wen Z. Deficiency of BrpB causes major defects in cell division, stress responses and biofilm formation by Streptococcus mutans. Microbiology. 2014;160:67-78 pubmed publisher
    ..mutans. Further studies are under way to elucidate the mechanism that underlies the BrpA- and BrpB-mediated regulation. ..
  66. Colby S, Whiting G, Tao L, Russell R. Insertional inactivation of the Streptococcus mutans dexA (dextranase) gene results in altered adherence and dextran catabolism. Microbiology. 1995;141 ( Pt 11):2929-36 pubmed publisher
    ..The mutants were also more adherent to a smooth surface than LT11 but there was no apparent difference in sucrose-dependent cell-cell aggregation.(ABSTRACT TRUNCATED AT 250 WORDS)..
  67. Gutierrez J, Crowley P, Brown D, Hillman J, Youngman P, Bleiweis A. Insertional mutagenesis and recovery of interrupted genes of Streptococcus mutans by using transposon Tn917: preliminary characterization of mutants displaying acid sensitivity and nutritional requirements. J Bacteriol. 1996;178:4166-75 pubmed
    ..This represents the first published report of successful Tn9l7 mutagenesis in the genus Streptococcus...
  68. Cobessi D, T te Favier F, Marchal S, Azza S, Branlant G, Aubry A. Apo and holo crystal structures of an NADP-dependent aldehyde dehydrogenase from Streptococcus mutans. J Mol Biol. 1999;290:161-73 pubmed publisher
    ..mutans ALDH. It was found that Arg103, Arg283 and Asp440 might be key residues for substrate binding...
  69. Qi F, Chen P, Caufield P. Purification and biochemical characterization of mutacin I from the group I strain of Streptococcus mutans, CH43, and genetic analysis of mutacin I biosynthesis genes. Appl Environ Microbiol. 2000;66:3221-9 pubmed
    ..Comparison of the primary sequence of mutacin I with that of mutacin III and epidermin suggests that mutacin I likely has the same bridging pattern as epidermin...
  70. Boyd D, Cvitkovitch D, Bleiweis A, Kiriukhin M, Debabov D, Neuhaus F, et al. Defects in D-alanyl-lipoteichoic acid synthesis in Streptococcus mutans results in acid sensitivity. J Bacteriol. 2000;182:6055-65 pubmed
    ..Proton permeability assays revealed that the mutant was more permeable to protons than the wild type. This observation suggests a mechanism for the loss of the characteristic acid tolerance response in S. mutans...
  71. Chia J, Chang L, Shun C, Chang Y, Tsay Y, Chen J. A 60-kilodalton immunodominant glycoprotein is essential for cell wall integrity and the maintenance of cell shape in Streptococcus mutans. Infect Immun. 2001;69:6987-98 pubmed publisher
    ..These results suggested that IDG-60 is essential for maintaining the integrity of the cell wall and the uniformity of cell shape, both of which are indispensable for bacteria survival under stress conditions...
  72. Igarashi T, Asaga E, Goto N. The sortase of Streptococcus mutans mediates cell wall anchoring of a surface protein antigen. Oral Microbiol Immunol. 2003;18:266-9 pubmed
    ..Western blot analysis with specific antiserum showed that the 200 kDa protein was a surface protein antigen designated PAc. These results suggest that the sortase catalyzes anchoring of the antigen PAc to the cell wall...
  73. Veiga Malta I, Duarte M, Dinis M, Madureira P, Ferreira P, Videira A. Identification of NAD+ synthetase from Streptococcus sobrinus as a B-cell-stimulatory protein. J Bacteriol. 2004;186:419-26 pubmed
    ..Stimulation with the recombinant NAD(+) synthetase was also observed with other B-cell markers, such as CD19(+), B220(+), and CD21(+). Cell proliferation follows the activation induced by the recombinant NAD(+) synthetase...
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    ..The identical autolytic profile was obtained using a sortase mutant (SrtA(-))...
  75. Arimoto T, Igarashi T. Role of prolipoprotein diacylglyceryl transferase (Lgt) and lipoprotein-specific signal peptidase II (LspA) in localization and physiological function of lipoprotein MsmE in Streptococcus mutans. Oral Microbiol Immunol. 2008;23:515-9 pubmed publisher
    ..mutans. These results demonstrate that MsmE is required for melibiose metabolism in S. mutans and that modification by Lgt and LspA are important processes for the physiological function of MsmE...
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    ..The role of this oligomerization state for ComE function is discussed...
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    ..This is the first report that describes a role for S. mutans' CinA in contending with DNA damage, genetic transformation and cell survival...
  78. Zeng L, Burne R. Comprehensive mutational analysis of sucrose-metabolizing pathways in Streptococcus mutans reveals novel roles for the sucrose phosphotransferase system permease. J Bacteriol. 2013;195:833-43 pubmed publisher
    ..Sucrose-dependent catabolite repression was also evident in strains containing an intact sucrose PTS. Collectively, these results reveal a novel regulatory circuitry for the control of sucrose catabolism, with a central role for ScrA...
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    ..mutans because of the capacity of this approach to yield a more precise landscape of transcriptomic changes in response to specific mutations and growth conditions. ..
  80. De A, Lupidi G, Petrelli D, Vitali L. Molecular cloning and biochemical characterization of Xaa-Pro dipeptidyl-peptidase from Streptococcus mutans and its inhibition by anti-human DPP IV drugs. FEMS Microbiol Lett. 2016;363: pubmed publisher
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    ..This suggests that a family of cell surface proteins which, by analogy with the 185,000-Mr SA I/II of S. mutans, could be involved in bacterial adhesion might exist...
  82. Boyd D, Thevenot T, Gumbmann M, Honeyman A, Hamilton I. Identification of the operon for the sorbitol (Glucitol) Phosphoenolpyruvate:Sugar phosphotransferase system in Streptococcus mutans. Infect Immun. 2000;68:925-30 pubmed
    ..Arnaud, G. Rapoport, and I. Martin-Verstraete, Mol. Microbiol. 28:865-874, 1998), and its gene overlaps the srlM gene by 1 bp. The arrangement of these two regulatory genes is unique, having not been reported for other bacteria...
  83. Asanuma N, Yoshii T, Hino T. Molecular characteristics and transcription of the gene encoding a multifunctional alcohol dehydrogenase in relation to the deactivation of pyruvate formate-lyase in the ruminal bacterium Streptococcus bovis. Arch Microbiol. 2004;181:122-8 pubmed publisher
    ..Partition of the flow from pyruvate appears to be mainly regulated by the activities of lactate dehydrogenase and PFL...
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    ..Also, Ser102 in SmIDH is a potential phosphorylation site, indicating that the ancient NAD-dependent IDHs might be the underlying origin of "phosphorylation mechanism" used by their bacterial NADP-dependent homologs...
  86. Wang Z, Li L, Dong Y, Su X. Structural and biochemical characterization of MdaB from cariogenic Streptococcus mutans reveals an NADPH-specific quinone oxidoreductase. Acta Crystallogr D Biol Crystallogr. 2014;70:912-21 pubmed publisher
    ..Two strongly basic patches near the substrate pocket are expected to confer the preference for NADPH over NADH. These studies shed light on future drug development against the cariogenic pathogen S. mutans...
  87. Burne R, Penders J. Characterization of the Streptococcus mutans GS-5 fruA gene encoding exo-beta-D-fructosidase. Infect Immun. 1992;60:4621-32 pubmed
    ..The data begin to define functional domains of the FruA protein and potential regulatory sites for induction, repression, growth rate control, and posttranslational localization of this multifunctional enzyme...
  88. Rathsam C, Giffard P, Jacques N. The cell-bound fructosyltransferase of Streptococcus salivarius: the carboxyl terminus specifies attachment in a Streptococcus gordonii model system. J Bacteriol. 1993;175:4520-7 pubmed
    ..This cell-bound activity was released in the presence of sucrose, suggesting that the mode of attachment and release of the S. salivarius FTF in S. gordonii was similar to that in its native host...
  89. Morona J, Morona R, Paton J. Characterization of the locus encoding the Streptococcus pneumoniae type 19F capsular polysaccharide biosynthetic pathway. Mol Microbiol. 1997;23:751-63 pubmed
    ..pneumoniae serotypes/groups tested. The region from cps19fG to cps19fK was found only in members of serogroup 19, and, within this, cps19fl was unique to type 19F...
  90. Stinson M, McLaughlin R, Choi S, Juarez Z, Barnard J. Streptococcal histone-like protein: primary structure of hlpA and protein binding to lipoteichoic acid and epithelial cells. Infect Immun. 1998;66:259-65 pubmed
    ..These results support a potential role for HlpA in the pathogenesis of streptococcus-induced tissue inflammation...
  91. Morona J, Morona R, Paton J. Comparative genetics of capsular polysaccharide biosynthesis in Streptococcus pneumoniae types belonging to serogroup 19. J Bacteriol. 1999;181:5355-64 pubmed
  92. Kitten T, Munro C, Michalek S, Macrina F. Genetic characterization of a Streptococcus mutans LraI family operon and role in virulence. Infect Immun. 2000;68:4441-51 pubmed
    ..Virulence for endocarditis was restored by correction of the sloC mutation but not by provision of the sloC gene in trans, suggesting that virulence requires the expression of other genes in the sloC operon...