Staphylococcus aureus subsp. aureus N315

Summary

Alias: Staphylococcus aureus (strain N315)

Top Publications

  1. ncbi Two-component system VraSR positively modulates the regulation of cell-wall biosynthesis pathway in Staphylococcus aureus
    Makoto Kuroda
    Bacteriology, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Mol Microbiol 49:807-21. 2003
  2. ncbi Passage of heme-iron across the envelope of Staphylococcus aureus
    Sarkis K Mazmanian
    Committee on Microbiology, Department of Molecular Genetics and Cell Biology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Science 299:906-9. 2003
  3. pmc MsrR, a putative cell envelope-associated element involved in Staphylococcus aureus sarA attenuation
    Jutta Rossi
    Institute of Medical Microbiology, University of Zurich, CH 8028 Zurich, Switzerland
    Antimicrob Agents Chemother 47:2558-64. 2003
  4. pmc Sigma-B, a putative operon encoding alternate sigma factor of Staphylococcus aureus RNA polymerase: molecular cloning and DNA sequencing
    S Wu
    The Rockefeller University, New York, New York 10021, USA
    J Bacteriol 178:6036-42. 1996
  5. ncbi Immune evasion by a staphylococcal complement inhibitor that acts on C3 convertases
    Suzan H M Rooijakkers
    Eijkman Winkler Institute, University Medical Center Utrecht, G04 614, 3584 CX Utrecht, The Netherlands
    Nat Immunol 6:920-7. 2005
  6. ncbi Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore
    L Song
    Department of Biochemistry, University of Chicago, 920 East 58 Street, Chicago, IL 60637, USA
    Science 274:1859-66. 1996
  7. pmc The Staphylococcus aureus protein Sbi acts as a complement inhibitor and forms a tripartite complex with host complement Factor H and C3b
    Katrin Haupt
    Department of Infection Biology, Leibniz Institute for Natural Product Research and Infection Biology, Jena, Germany
    PLoS Pathog 4:e1000250. 2008
  8. ncbi Heme coordination by Staphylococcus aureus IsdE
    Jason C Grigg
    Department of Microbiology and Immunology, Life Sciences Institute, The University of British Columbia, Vancouver, British Columbia, Canada
    J Biol Chem 282:28815-22. 2007
  9. ncbi Shuttle vectors derived from pN315 for study of essential genes in Staphylococcus aureus
    Miki Matsuo
    Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Biol Pharm Bull 33:198-203. 2010
  10. ncbi Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth
    Alexander Scherl
    Biomedical Proteomics Research Group, Geneva University Hospitals, Geneva, Switzerland
    J Microbiol Methods 60:247-57. 2005

Research Grants

  1. Gordon Research Conference on Staphylococcal Diseases
    BRIGITTE BERGER BACHI; Fiscal Year: 2003

Scientific Experts

Detail Information

Publications81

  1. ncbi Two-component system VraSR positively modulates the regulation of cell-wall biosynthesis pathway in Staphylococcus aureus
    Makoto Kuroda
    Bacteriology, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Mol Microbiol 49:807-21. 2003
    ..The observation strongly indicates that VraSR constitutes a positive regulator of cell-wall peptidoglycan synthesis, and that is deeply involved in the expression of beta-lactam and glycopeptide resistance in S. aureus...
  2. ncbi Passage of heme-iron across the envelope of Staphylococcus aureus
    Sarkis K Mazmanian
    Committee on Microbiology, Department of Molecular Genetics and Cell Biology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Science 299:906-9. 2003
    ..Thus, Isd appears to act as an import apparatus that uses cell wall-anchored proteins to relay heme-iron across the bacterial envelope...
  3. pmc MsrR, a putative cell envelope-associated element involved in Staphylococcus aureus sarA attenuation
    Jutta Rossi
    Institute of Medical Microbiology, University of Zurich, CH 8028 Zurich, Switzerland
    Antimicrob Agents Chemother 47:2558-64. 2003
    ..These observations suggest that MsrR is a new component involved in sarA attenuation and the regulatory network controlling virulence gene expression in S. aureus...
  4. pmc Sigma-B, a putative operon encoding alternate sigma factor of Staphylococcus aureus RNA polymerase: molecular cloning and DNA sequencing
    S Wu
    The Rockefeller University, New York, New York 10021, USA
    J Bacteriol 178:6036-42. 1996
    ..subtilis sigB operon. A Tn551 insertional mutant, RUSA168 (insert in orf256 of the staphylococcal sigma-B operon), showed drastic reduction in methicillin resistance (decrease in MIC from 1,600 microg ml-1 to 12 to 25 microg ml-1off..
  5. ncbi Immune evasion by a staphylococcal complement inhibitor that acts on C3 convertases
    Suzan H M Rooijakkers
    Eijkman Winkler Institute, University Medical Center Utrecht, G04 614, 3584 CX Utrecht, The Netherlands
    Nat Immunol 6:920-7. 2005
    ..As a highly active and small soluble protein that acts exclusively on surfaces, staphylococcal complement inhibitor may represent a promising anti-inflammatory molecule...
  6. ncbi Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore
    L Song
    Department of Biochemistry, University of Chicago, 920 East 58 Street, Chicago, IL 60637, USA
    Science 274:1859-66. 1996
    ....
  7. pmc The Staphylococcus aureus protein Sbi acts as a complement inhibitor and forms a tripartite complex with host complement Factor H and C3b
    Katrin Haupt
    Department of Infection Biology, Leibniz Institute for Natural Product Research and Infection Biology, Jena, Germany
    PLoS Pathog 4:e1000250. 2008
    ..Thus, Sbi is a multifunctional bacterial protein, which binds host complement components Factor H and C3 as well as IgG and beta(2)-glycoprotein I and interferes with innate immune recognition...
  8. ncbi Heme coordination by Staphylococcus aureus IsdE
    Jason C Grigg
    Department of Microbiology and Immunology, Life Sciences Institute, The University of British Columbia, Vancouver, British Columbia, Canada
    J Biol Chem 282:28815-22. 2007
    ..aureus IsdE with respect to heme binding and transport...
  9. ncbi Shuttle vectors derived from pN315 for study of essential genes in Staphylococcus aureus
    Miki Matsuo
    Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Biol Pharm Bull 33:198-203. 2010
    ..In contrast, other two genes were shown to be dispensable by this system. Thus, the pN315-based plasmids appear to be useful for studying genes essential for S. aureus cell growth...
  10. ncbi Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth
    Alexander Scherl
    Biomedical Proteomics Research Group, Geneva University Hospitals, Geneva, Switzerland
    J Microbiol Methods 60:247-57. 2005
    ..The simultaneous large-scale analysis of transcriptomes and proteomes enables a global and holistic view of the S. aureus biology, allowing the parallel study of multiple active events in an organism...
  11. ncbi Platelet-binding domains in 2 fibrinogen-binding proteins of Staphylococcus aureus identified by phage display
    Christine Heilmann
    Institute of Medical Microbiology, University of Munster, D 48149 Munster, Germany
    J Infect Dis 186:32-9. 2002
    ..The surface-associated fraction of the C-terminal domain of coagulase or the N-terminal domain of Efb may be involved in bacterial adherence to immobilized platelets, and fibrinogen may act as a bridging molecule in that interaction...
  12. pmc Structures of the pleiotropic translational regulator Hfq and an Hfq-RNA complex: a bacterial Sm-like protein
    Maria A Schumacher
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, OR 97201 3098, USA
    EMBO J 21:3546-56. 2002
    ..Such binding suggests a mechanism for Hfq function...
  13. ncbi The crystal structures of four peptide deformylases bound to the antibiotic actinonin reveal two distinct types: a platform for the structure-based design of antibacterial agents
    Jean Pierre Guilloteau
    Drug Innovation and Approval, Aventis Pharma, 13 quai Jules Guesde, BP 14, F 94403, Vitry sur Seine, France
    J Mol Biol 320:951-62. 2002
    ....
  14. ncbi Expression, purification, and characterization of peptidyl-tRNA hydrolase from Staphylococcus aureus
    Paul D Bonin
    Cell and Molecular Biology, Rockville, Maryland, USA
    Protein Expr Purif 24:123-30. 2002
    ..aureus Pth was determined to be 2.8 microM. A far UV circular dichroism spectrum revealed that His-tagged S. aureus Pth appears to have a structured core predominated by beta-sheet...
  15. ncbi Arsenate reductase from S. aureus plasmid pI258 is a phosphatase drafted for redox duty
    I Zegers
    Dienst Ultrastruktuur, Vlaams Interuniversitair Instituut voor Biotechnologie VIB, Vrije Universiteit Brussel, Paardenstraat 65, B 1640 St Genesius Rode, Belgium
    Nat Struct Biol 8:843-7. 2001
    ..The reaction is associated with major structural changes in the ArsC...
  16. pmc Influence of a functional sigB operon on the global regulators sar and agr in Staphylococcus aureus
    M Bischoff
    Institute of Medical Microbiology, University of Zurich, CH 8028 Zurich, Switzerland
    J Bacteriol 183:5171-9. 2001
    ..The data presented here suggest that sigma(B) increases sar expression while simultaneously reducing the RNAIII level in a growth phase-dependent manner...
  17. pmc Cloning and expression of cadD, a new cadmium resistance gene of Staphylococcus aureus
    S S Crupper
    Division of Biological Sciences, Emporia State University, Emporia, Kansas 66801, USA
    J Bacteriol 181:4071-5. 1999
    ..Moreover, the truncated version of cadX contained in pRW001 is nonfunctional and may have been generated by deletion during recombination to acquire the cadmium resistance element...
  18. pmc An unusual isopentenyl diphosphate isomerase found in the mevalonate pathway gene cluster from Streptomyces sp. strain CL190
    K Kaneda
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 98:932-7. 2001
    ..aureus and of the different enzymatic properties of mammalian (type 1) and S. aureus (type 2) isomerases, this unusual enzyme is considered to be a suitable molecular target for the screening of antibacterial drugs specific to S. aureus...
  19. pmc Identification and characterization of a membrane permease involved in iron-hydroxamate transport in Staphylococcus aureus
    M T Sebulsky
    Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada, N6A 5C1
    J Bacteriol 182:4394-400. 2000
    ..By using the Fur titration assay, it was shown that the Fur box sequences upstream of fhuCBG are recognized by the Escherichia coli Fur protein...
  20. ncbi The essential catalytic redox couple in arsenate reductase from Staphylococcus aureus
    J Messens
    Dienst Ultrastructuur, Vlaams Interuniversitair Instituut voor Biotechnologie, Vrije Universiteit Brussel, Belgium
    Biochemistry 38:16857-65. 1999
    ..From the peptide-mass profiles, cysteines 82 and 89 were identified as the redox couple of ArsC necessary to reduce arsenate to arsenite...
  21. ncbi Topological analysis of the MraY protein catalysing the first membrane step of peptidoglycan synthesis
    A Bouhss
    Biochimie Structurale et Cellulaire, CNRS, Batiment 430, Universite Paris Sud, F 91405, Orsay, France
    Mol Microbiol 34:576-85. 1999
    ..It was noteworthy that S. aureus MraY was functional in E. coli. An increased MraY transferase activity was observed only with the untruncated hybrids from both organisms...
  22. ncbi Identification, cloning, and expression of a functional phenylalanyl-tRNA synthetase (pheRS) from Staphylococcus aureus
    J W Savopoulos
    GlaxoSmithKline Pharmaceuticals, New Frontiers Science Park North, Coldharbour Road, Harlow, Essex CM19 5AD, United Kingdom
    Protein Expr Purif 21:470-84. 2001
    ..aureus (Oxford Strain) and demonstrate that Michaelis-Menten parameters for both recombinant and native enzyme, at least for phenylalanine tRNA aminoacylation are comparable...
  23. pmc Characterization of NorR protein, a multifunctional regulator of norA expression in Staphylococcus aureus
    Que Chi Truong-Bolduc
    Division of Infectious Diseases and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114 2696, USA
    J Bacteriol 185:3127-38. 2003
    ..Collectively, these results indicate that NorR is a multifunctional regulator, affecting cell surface properties as well as the expression of NorA and likely other multidrug resistance efflux pumps...
  24. pmc A novel DNA-binding protein modulating methicillin resistance in Staphylococcus aureus
    Miriam Ender
    Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland
    BMC Microbiol 9:15. 2009
    ..Here we searched for novel proteins binding to the mec operator, in an attempt to identify new factor(s) controlling methicillin resistance phenotypes...
  25. ncbi Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A
    Satoshi Sato
    MRC Centre for Protein Engineering, Hills Road, Cambridge CB2 2QH, UK
    J Mol Biol 372:254-67. 2007
    ..The results suggest that BdpA folds via a single dominant folding pathway...
  26. pmc Inactivation of traP has no effect on the agr quorum-sensing system or virulence of Staphylococcus aureus
    Lindsey N Shaw
    Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65211, USA
    Infect Immun 75:4519-27. 2007
    ..Finally, analysis conducted using a murine model of S. aureus septic arthritis revealed that, in contrast to an agr mutant, the traP mutant was just as virulent as the wild-type strain...
  27. pmc Cloning, purification and preliminary crystallographic analysis of a conserved hypothetical protein, SA0961 (YlaN), from Staphylococcus aureus
    Ling Xu
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2TN, England
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:778-80. 2006
    ..5, b = 42.7, c = 62.7 angstroms, beta = 92.4 degrees and two molecules in the asymmetric unit. A full structure determination is under way to provide insights into the function of this protein...
  28. ncbi The structure of the C5a receptor-blocking domain of chemotaxis inhibitory protein of Staphylococcus aureus is related to a group of immune evasive molecules
    Pieter Jan Haas
    Eijkman Winkler Institute, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    J Mol Biol 353:859-72. 2005
    ..Its structural homology to S.aureus SSLs, superantigens, and EAP might help the design of future experiments towards an understanding of the relationship between structure and function of these proteins...
  29. pmc Successful multiresistant community-associated methicillin-resistant Staphylococcus aureus lineage from Taipei, Taiwan, that carries either the novel Staphylococcal chromosome cassette mec (SCCmec) type VT or SCCmec type IV
    Susan Boyle-Vavra
    Department of Pediatrics, Section of Infectious Diseases, University of Chicago, IL, 60637, USA
    J Clin Microbiol 43:4719-30. 2005
    ..SCCmec V(T) is a novel SCCmec variant that is found in multiply resistant CAMRSA strains with sequence type 59 in Taipei in association with the PVL leukotoxin genes...
  30. pmc Nucleotide sequence of the type C3 staphylococcal enterotoxin gene suggests that intergenic recombination causes antigenic variation
    J L Couch
    Department of Bacteriology, University of Wisconsin Madison 53706
    J Bacteriol 171:4507-10. 1989
    ..Sequence comparisons between the entC3, entC1, and entB genes suggest that an ancestral entC1-like gene was formed by recombination between the entC3 and entB genes...
  31. ncbi Structure and biosynthesis of staphyloxanthin from Staphylococcus aureus
    Alexandra Pelz
    Department of Microbial Genetics, University of Tuebingen, Germany
    J Biol Chem 280:32493-8. 2005
    ..Staphyloxanthin was identified as beta-D-glucopyranosyl 1-O-(4,4'-diaponeurosporen-4-oate)-6-O-(12-methyltetradecanoate)...
  32. ncbi Crystal structures of the Apo and penicillin-acylated forms of the BlaR1 beta-lactam sensor of Staphylococcus aureus
    Mark S Wilke
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 279:47278-87. 2004
    ....
  33. pmc Regulatory elements of the Staphylococcus aureus protein A (Spa) promoter
    Jinxin Gao
    Department of Diagnostic Medicine Pathobiology, College of Veterinary Medicine, Kansas State University, 1800 Denison Ave, Manhattan, KS 66506, USA
    J Bacteriol 186:3738-48. 2004
    ..Full repression required the presence of a second consensus site adjacent to the SarS binding site. Sequences directly upstream of the core promoter sequence were found to stimulate transcription...
  34. ncbi Genetic analysis of 17 genes in Staphylococcus aureus with reduced susceptibility to vancomycin (VISA) and heteroVISA
    M Wootton
    J Antimicrob Chemother 53:406-7. 2004
  35. pmc SarA positively controls bap-dependent biofilm formation in Staphylococcus aureus
    Maria Pilar Trotonda
    Instituto Valenciano de Investigaciones Agrarias, Carretera Náquera Moncada Km 4, 5, 46113 Moncada, Valencia, Spain
    J Bacteriol 187:5790-8. 2005
    ..Based on the previous studies of others as well as our work demonstrating the role for SarA in icaADBC and bap expression, we propose that SarA is an essential regulator controlling biofilm formation in S. aureus...
  36. pmc Crystal structure of the SarS protein from Staphylococcus aureus
    Ronggui Li
    Integrated Department of Immunology, National Jewish Medical and Research Center, and Department of Pharmacology, Biomolecular Structure Program, School of Medicine, University of Colorado Health Science Center, Denver, Colorado 80206, USA
    J Bacteriol 185:4219-25. 2003
    ..Based on the structural differences of SarR, SarS, and MarR, we further classified these winged-helix proteins to three subfamilies, SarA, SarS, and MarR. Finally, a possible transcription regulation mechanism is proposed...
  37. ncbi Antiseptic susceptibility and distribution of antiseptic-resistance genes in methicillin-resistant Staphylococcus aureus
    N Noguchi
    Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan
    FEMS Microbiol Lett 172:247-53. 1999
    ..Our results indicated that four or more antiseptic-resistance genes exist in methicillin-resistant S. aureus and that antiseptic-resistant methicillin-resistant S. aureus strains without qacA and smr are widely spread in Japan...
  38. pmc Identification and characterization of SirA, an iron-regulated protein from Staphylococcus aureus
    J H Heinrichs
    MedImmune, Inc, Gaithersburg, Maryland 20878, USA
    J Bacteriol 181:1436-43. 1999
    ..aureus culture medium. sir-encoded proteins may be involved in iron acquisition in vivo and therefore may be targets for antimicrobial agents...
  39. ncbi An engineered Staphylococcus aureus PC1 beta-lactamase that hydrolyses third-generation cephalosporins
    L E Zawadzke
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville 20850, USA
    Protein Eng 8:1275-85. 1995
    ..aureus beta-lactamase structure, widening the active site cleft and providing space to accommodate the bulky side-chains of the third-generation cephalosporins...
  40. pmc Histidine residues near the N terminus of staphylococcal alpha-toxin as reporters of regions that are critical for oligomerization and pore formation
    R Jursch
    Institute of Medical Microbiology, University of Mainz, Germany
    Infect Immun 62:2249-56. 1994
    ..We interpret these results to indicate that the N-terminal domain of alpha-toxin in the region of H-35 and H-48 is involved in protomer-protomer interactions that underlie the hexamerization and pore-forming process...
  41. pmc Primary sequence of the alpha-toxin gene from Staphylococcus aureus wood 46
    G S Gray
    Infect Immun 46:615-8. 1984
    ..The mature alpha-toxin protein had a molecular size of 33,000 and contained only three short regions of high hydrophobicity in addition to a number of short, weakly hydrophobic regions...
  42. ncbi Molecular evolution of the staphylococcal and streptococcal pyrogenic toxin gene family
    R A Van Den Bussche
    Department of Biological Sciences, University of Idaho, Moscow 83843
    Mol Phylogenet Evol 2:281-92. 1993
    ....
  43. ncbi Plasmid-determined bleomycin resistance in Staphylococcus aureus
    D Semon
    Plasmid 17:46-53. 1987
    ..The bleomycin resistance determinant of the gram-positive plasmid pUB110 was compared to that of the enterobacterial transposon Tn5; limited regions of close relatedness could be identified...
  44. ncbi Identification of a chromosomally encoded ABC-transport system with which the staphylococcal erythromycin exporter MsrA may interact
    J I Ross
    Department of Microbiology, University of Leeds, UK
    Gene 153:93-8. 1995
    ..There was no further sequence similarity outside these conserved regions. These results indicate that the chromosomes of S. hominis and S. aureus contain sequences encoding a potential TM protein with which MsrA might interact...
  45. pmc A Staphylococcus aureus autolysin that has an N-acetylmuramoyl-L-alanine amidase domain and an endo-beta-N-acetylglucosaminidase domain: cloning, sequence analysis, and characterization
    T Oshida
    Rockefeller University, New York, NY 10021
    Proc Natl Acad Sci U S A 92:285-9. 1995
    ..aureus...
  46. pmc Regulation of exoprotein expression in Staphylococcus aureus by a locus (sar) distinct from agr
    A L Cheung
    Laboratory of Bacterial Pathogenesis and Immunology, Rockefeller University, New York, NY 10021
    Proc Natl Acad Sci U S A 89:6462-6. 1992
    ..aureus strain with a genetic background similar to strain 8325-4. This locus on the S. aureus chromosome, possibly regulatory in nature, has been designated sar for staphylococcal accessory regulator...
  47. ncbi Identification of the active-site nucleophile in 6-phospho-beta-galactosidase from Staphylococcus aureus by labelling with synthetic inhibitors
    P Staedtler
    Arbeitsgruppe Physiologie der Mikroorganismen, Abteilung Biologie, Ruhr Universitat Bochum, Germany
    Eur J Biochem 232:658-63. 1995
    ..It was shown to be identical to an authentic, synthetic sample. From this, it is evident that E375 is the active-site nucleophile of 6-phospho-galactosidase, consistent with previous findings for enzymes in this family...
  48. ncbi A homologue to the Escherichia coli alkyl hydroperoxide reductase AhpC is induced by osmotic upshock in Staphylococcus aureus
    L Armstrong-Buisseret
    Department of Applied Biochemistry and Food Science, University of Nottingham, Faculty of Agricultural and Food Sciences, Leicestershire, UK
    Microbiology 141:1655-61. 1995
    ..Exposure of S. aureus to varying concentrations of H202 did not result in the detectable induction of AhpC...
  49. ncbi The phosphoenolpyruvate:sugar phosphotransferase system in gram-positive bacteria: properties, mechanism, and regulation
    J Reizer
    Department of Biology, University of California at San Diego, La Jolla
    Crit Rev Microbiol 15:297-338. 1988
    ....
  50. ncbi Amino acid sequence of the amphiphilic phosphocarrier protein factor IIILac of the lactose-specific phosphotransferase system of Staphylococcus
    K Stuber
    Biochemistry 24:1164-8. 1985
    ..The N-terminal part of the protein comprising approximately one-third of the chain exhibits in vitro affinity toward membrane-bound enzyme IILac...
  51. pmc Enzymatic and nucleotide sequence studies of a kanamycin-inactivating enzyme encoded by a plasmid from thermophilic bacilli in comparison with that encoded by plasmid pUB110
    M Matsumura
    J Bacteriol 160:413-20. 1984
    ..The difference in thermostability between the two enzymes caused by a single amino acid replacement is discussed in light of electrostatic effects...
  52. ncbi Nucleotide sequence of the staphylococcal enterotoxin C3 gene: sequence comparison of all three type C staphylococcal enterotoxins
    C J Hovde
    Department of Bacteriology and Biochemistry, University of Idaho, Moscow 83843
    Mol Gen Genet 220:329-33. 1990
    ....
  53. ncbi A point mutation in norA gene is responsible for quinolone resistance in Staphylococcus aureus
    Y Ohshita
    Department of Microbiology, Faculty of Medicine, Juntendo University, Tokyo, Japan
    Biochem Biophys Res Commun 172:1028-34. 1990
    ....
  54. ncbi Nucleotide sequences and biologic properties of toxic shock syndrome toxin 1 from ovine- and bovine-associated Staphylococcus aureus
    P K Lee
    Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455 0312
    J Infect Dis 165:1056-63. 1992
    ..Also, TSST-ovine was less reactive to certain monoclonal antibodies raised against TSST-1...
  55. ncbi Clumping factor B (ClfB), a new surface-located fibrinogen-binding adhesin of Staphylococcus aureus
    D Ni Eidhin
    Microbiology Department, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland
    Mol Microbiol 30:245-57. 1998
    ....
  56. ncbi The ternary microplasmin-staphylokinase-microplasmin complex is a proteinase-cofactor-substrate complex in action
    M A Parry
    Max Planck Institute of Biochemistry, Department for Structural Research, Martinsried, Germany
    Nat Struct Biol 5:917-23. 1998
    ..Furthermore, it provides a template for the design of improved plasminogen activators and plasmin inhibitors with considerable therapeutical potential...
  57. ncbi Characterization of a novel structural member, LukE-LukD, of the bi-component staphylococcal leucotoxins family
    A Gravet
    UPRES EA 1318, Institut de Bacteriologie de la Faculte de Medecine, Université Louis Pasteur Hôpitaux Universitaires de Strasbourg, France
    FEBS Lett 436:202-8. 1998
    ..005). LukE+LukD was as effective as the Panton-Valentine leucocidin for inducing dermonecrosis when injected in the rabbit skin, but not hemolytic and poorly leucotoxic compared to other leucotoxins expressed by Staphylococcus aureus...
  58. ncbi Comparative biochemical and molecular analysis of the Staphylococcus hyicus, Staphylococcus aureus and a hybrid lipase. Indication for a C-terminal phospholipase domain
    K Nikoleit
    Universitat Tubingen, Germany
    Eur J Biochem 228:732-8. 1995
    ..hyicus lipase. The fact that two closely related enzymes differ in the need for Ca2+ underscores the notion that it plays a structural rather than a catalytic role...
  59. ncbi Functional expression of the alpha-hemolysin of Staphylococcus aureus in intact Escherichia coli and in cell lysates. Deletion of five C-terminal amino acids selectively impairs hemolytic activity
    B Walker
    Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545
    J Biol Chem 267:10902-9. 1992
    ..The mutant polypeptide nevertheless binds tightly to erythrocytes as a monomer, strengthening the idea that oligomerization is required for cell lysis...
  60. pmc A general method for cloning recA genes of gram-positive bacteria by polymerase chain reaction
    P Duwat
    Laboratoire de Genetique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France
    J Bacteriol 174:5171-5. 1992
    ..This method is particularly useful for the recovery of the recA genes of gram-positive bacteria and avoids the difficulties of using a genetic complementation test for cloning...
  61. pmc Localized perforation of the cell wall by a major autolysin: atl gene products and the onset of penicillin-induced lysis of Staphylococcus aureus
    M Sugai
    Department of Microbiology, Hiroshima University School of Dentistry, Japan
    J Bacteriol 179:2958-62. 1997
    ....
  62. ncbi Chorismate synthase from Staphylococcus aureus
    M J Horsburgh
    Division of Biochemistry and Molecular Biology, University of Glasgow, UK
    Microbiology 142:2943-50. 1996
    ..The properties of S. aureus chorismate synthase are compared with those of the B. subtilis and E. coli enzymes...
  63. ncbi Structure and kinetics of the beta-lactamase mutants S70A and K73H from Staphylococcus aureus PC1
    C C Chen
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville 20850, USA
    Biochemistry 35:12251-8. 1996
    ..A crystal soaked with ampicillin was flash-frozen, and diffraction data were collected at 2.1 A resolution. The electron density map showed no indication of substrate binding...
  64. pmc Complete nucleotide sequence of IS431mec in Staphylococcus aureus
    L Barberis-Maino
    Institute of Medical Microbiology, University of Zurich, Switzerland
    Nucleic Acids Res 18:5548. 1990
  65. ncbi A genetic system for analysis of staphylococcal nuclease
    D Shortle
    Gene 22:181-9. 1983
    ..In combination with present methods for efficient in vitro mutagenesis, this plasmid-based genetic system can be applied to the detailed genetic analysis of this small, biochemically and biophysically well-characterized enzyme...
  66. ncbi Structural basis for the inactivation of the P54 mutant of beta-lactamase from Staphylococcus aureus PC1
    O Herzberg
    Center for Advanced Research in Biotechnology, Maryland Biotechnology Institute, University of Maryland, Rockville 20850
    Biochemistry 30:9503-9. 1991
    ..This disorder affects catalysis since some of the disordered residues, in particular Glu166, form part of the active site.(ABSTRACT TRUNCATED AT 250 WORDS)..
  67. ncbi A genetic and molecular characterization of the recA gene from Staphylococcus aureus
    K W Bayles
    Department of Biological Sciences, University of Maryland, Baltimore 21228
    Gene 147:13-20. 1994
    ..Furthermore, genetic and physical mapping of S. aureus recA placed it in the same region as the uvs-568 mutation. These data strongly suggest that these mutations represent different alleles of the same recA gene...
  68. pmc Transcriptional analysis of the Staphylococcus aureus penicillin binding protein 2 gene
    M G Pinho
    Laboratory of Microbiology, The Rockefeller University, New York, New York 10021, USA
    J Bacteriol 180:6077-81. 1998
    ..We now show that introduction of the intact pbp2 gene with its two newly identified promoters into the chromosome of the transposon mutant resulted in the full recovery of high-level methicillin resistance...
  69. ncbi Staphylococcus aureus clumping factor B (ClfB) promotes adherence to human type I cytokeratin 10: implications for nasal colonization
    Louise M O'Brien
    Moyne Institute of Preventive Medicine, Department of Microbiology, Trinity College Dublin, Dublin 2, Ireland
    Cell Microbiol 4:759-70. 2002
    ..We also showed that ClfB is transcribed by S. aureus in the human nares. We propose that ClfB is a major determinant in S. aureus nasal colonization...
  70. ncbi Modification of autolysis by synthetic peptides derived from the presumptive binding domain of Staphylococcus aureus autolysin
    M Takano
    Pharmaceutical Development Research Laboratory, Tanabe Seiyaku Co, Ltd, Saitama, Japan
    Microbiol Immunol 44:463-72. 2000
    ..aureus autolysin activities. These peptides, especially the 10-mer peptide B9 (PGTKLYTVPW) that represents the C-terminal half of A10 and N-terminal half of A11, may be important segments for Atl to bind to the cell surface...
  71. ncbi Characterization of a Staphylococcus aureus transposon, Tn5405, located within Tn5404 and carrying the aminoglycoside resistance genes, aphA-3 and aadE
    A Derbise
    National Reference Center for Staphylococci, Unité des Staphylocoques, Institut Pasteur, Paris, France
    Plasmid 35:174-88. 1996
    ..IS1182 was not detected in the DNA of 4 of the 17 tested MRSA isolates containing aphA-3 and resistant to streptomycin. Thus, aphA-3 and aadE genes are not disseminated only by Tn5405 or related transposons delimited by IS1182...
  72. pmc Novel non-mecA-containing staphylococcal chromosomal cassette composite island containing pbp4 and tagF genes in a commensal staphylococcal species: a possible reservoir for antibiotic resistance islands in Staphylococcus aureus
    Kanokporn Mongkolrattanothai
    Section of Pediatric Infectious Diseases, Department of Pediatrics, University of Chicago, Chicago, Illinois 60637, USA
    Antimicrob Agents Chemother 48:1823-36. 2004
    ..In view of the fact that SCC-CI was found in a commensal species, it may represent a reservoir for sequences involved in genetic shuffling between staphylococci and may contribute to the diversity found in SCC elements...
  73. ncbi Cleavage of human immunoglobulins by serine proteinase from Staphylococcus aureus
    L Prokesova
    Laboratory of Special Medical Microbiology and Immunology, 1st Medical Faculty, Charles University, Studnickova, Prague, Czechoslovakia
    Immunol Lett 31:259-65. 1992
    ..SP has to be considered as one of the virulence factors of S. aureus that may protect bacteria against the defence mechanisms of the host...
  74. ncbi Chromosome-determined zinc-responsible operon czr in Staphylococcus aureus strain 912
    M Kuroda
    Department of Microbiology, Institute of Basic Medical Sciences, and College of Medical Technology, University of Tsukuba, Ibaraki, Japan
    Microbiol Immunol 43:115-25. 1999
    ..25 mg per g dry weight. The findings indicated that the novel operon czrAB should play a role in the transportation of zinc across the cell membrane to maintain the proper intracellular concentration...
  75. ncbi Subcellular localization of the major autolysin, ATL and its processed proteins in Staphylococcus aureus
    H Komatsuzawa
    Department of Microbiology, Hiroshima University School of Dentistry, Japan
    Microbiol Immunol 41:469-79. 1997
    ..We suggest that the 138-kDa ATL undergoes processing through intermediate proteins (115- and 85-kDa proteins) to mature as the active cell cluster-dispersing enzymes 51-kDa GL and 62-kDa AM on the cell surface...
  76. ncbi Catalase deficiency in Staphylococcus aureus subsp. anaerobius is associated with natural loss-of-function mutations within the structural gene
    R Sanz
    Departamento Patologia Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain
    Microbiology 146:465-75. 2000
    ..aureus subsp. anaerobius, as deduced from results obtained with chimeric catalase constructs...
  77. pmc Novel type of staphylococcal cassette chromosome mec identified in community-acquired methicillin-resistant Staphylococcus aureus strains
    Xiao Xue Ma
    Department of Bacteriology, Juntendo University, Tokyo, Japan
    Antimicrob Agents Chemother 46:1147-52. 2002
    ..Consistent with the strains' susceptibilities to various non-beta-lactam antibiotics, the type IV SCCmec was devoid of any antibiotic resistance genes other than the mecA gene...
  78. ncbi Multiple mechanisms for the activation of human platelet aggregation by Staphylococcus aureus: roles for the clumping factors ClfA and ClfB, the serine-aspartate repeat protein SdrE and protein A
    Louise O'Brien
    Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland
    Mol Microbiol 44:1033-44. 2002
    ..aureus. Thus, S. aureus has multiple mechanisms for stimulating platelet aggregation. Such functional redundancy suggests that this phenomenon may be important in the pathogenesis of invasive diseases such as infective endocarditis...
  79. ncbi Three-dimensional structure of MecI. Molecular basis for transcriptional regulation of staphylococcal methicillin resistance
    Raquel García-Castellanos
    Institut de Biologia Molecular de Barcelona, Centre d Investigació i Desenvolupament Consell Superior d Investigacions Científiques C Jordi Girona, 18 26, 08034 Barcelona, Spain
    J Biol Chem 278:39897-905. 2003
    ....
  80. pmc Transcriptional regulation of the Staphylococcus aureus thioredoxin and thioredoxin reductase genes in response to oxygen and disulfide stress
    Orit Uziel
    Department of Molecular Microbiology and Biotechnology, George S Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Israel
    J Bacteriol 186:326-34. 2004
    ..This observation, coupled with structural differences between the bacterial and mammalian thioredoxin reductases, suggests that it may serve as a target for the development of new antimicrobials...
  81. ncbi Nucleotide sequence of a spectinomycin adenyltransferase AAD(9) determinant from Staphylococcus aureus and its relationship to AAD(3") (9)
    E Murphy
    Mol Gen Genet 200:33-9. 1985
    ..The predicted amino acid sequence is 36% homologous to that of a widely distributed, gram-negative streptomycin/spectinomycin adenyltransferase, AAD(3") (9), specified by the aadA determinant (Holingshead and Vapnek 1985)...

Research Grants1

  1. Gordon Research Conference on Staphylococcal Diseases
    BRIGITTE BERGER BACHI; Fiscal Year: 2003
    ..S. This will afford a unique and rich opportunity for fellowship and scientific exchange between young and established investigators in the field. ..