Staphylococcus aureus subsp. aureus COL

Summary

Alias: Staphylococcus aureus COL

Top Publications

  1. pmc Cloning, characterization, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus
    H L Peng
    Public Health Research Institute, New York, New York 10016
    J Bacteriol 170:4365-72. 1988
  2. ncbi Identification and characterization of SarH1, a new global regulator of virulence gene expression in Staphylococcus aureus
    K Tegmark
    Microbiology and Tumorbiology Center MTC, Box 280, Karolinska Institutet, S 17177 Stockholm, Sweden
    Mol Microbiol 37:398-409. 2000
  3. pmc Transcriptional induction of the penicillin-binding protein 2 gene in Staphylococcus aureus by cell wall-active antibiotics oxacillin and vancomycin
    Susan Boyle-Vavra
    Department of Pediatrics, The University of Chicago, Illinois 60637, USA
    Antimicrob Agents Chemother 47:1028-36. 2003
  4. pmc Staphylococcus aureus resistance to human defensins and evasion of neutrophil killing via the novel virulence factor MprF is based on modification of membrane lipids with l-lysine
    A Peschel
    Microbial Genetics, University of Tuebingen, 72076 Tuebingen, Germany
    J Exp Med 193:1067-76. 2001
  5. ncbi Nucleotide sequence of the Staphylococcus aureus signal peptidase II (lsp) gene
    X J Zhao
    Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, MD 20899 4799
    FEBS Lett 299:80-4. 1992
  6. pmc DNA microarray-based identification of genes associated with glycopeptide resistance in Staphylococcus aureus
    Longzhu Cui
    Department of Bacteriology, Faculty of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Antimicrob Agents Chemother 49:3404-13. 2005
  7. pmc Mgr, a novel global regulator in Staphylococcus aureus
    Thanh T Luong
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    J Bacteriol 185:3703-10. 2003
  8. ncbi Three-dimensional structure of the complex between a T cell receptor beta chain and the superantigen staphylococcal enterotoxin B
    H Li
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville 20850, USA
    Immunity 9:807-16. 1998
  9. pmc Intranasal exposure to bacterial superantigens induces airway inflammation in HLA class II transgenic mice
    Govindarajan Rajagopalan
    Department of Immunology, Mayo Clinic College of Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Infect Immun 74:1284-96. 2006
  10. pmc A novel loop domain in superantigens extends their T cell receptor recognition site
    Sebastian Günther
    Boston Biomedical Research Institute, Watertown, MA 02472, USA
    J Mol Biol 371:210-21. 2007

Research Grants

Scientific Experts

Detail Information

Publications91

  1. pmc Cloning, characterization, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus
    H L Peng
    Public Health Research Institute, New York, New York 10016
    J Bacteriol 170:4365-72. 1988
    ....
  2. ncbi Identification and characterization of SarH1, a new global regulator of virulence gene expression in Staphylococcus aureus
    K Tegmark
    Microbiology and Tumorbiology Center MTC, Box 280, Karolinska Institutet, S 17177 Stockholm, Sweden
    Mol Microbiol 37:398-409. 2000
    ..In conclusion, we show that both the agr-dependent suppression of protein A production and the sarA-dependent stimulation of alpha-toxin production is mediated via a new regulator, SarH1, which belongs to a family of Sar homologues...
  3. pmc Transcriptional induction of the penicillin-binding protein 2 gene in Staphylococcus aureus by cell wall-active antibiotics oxacillin and vancomycin
    Susan Boyle-Vavra
    Department of Pediatrics, The University of Chicago, Illinois 60637, USA
    Antimicrob Agents Chemother 47:1028-36. 2003
    ..These data support the idea that pbpB expression is modulated by a trans-acting factor in response to the presence of the cell wall-active antibiotics vancomycin and oxacillin...
  4. pmc Staphylococcus aureus resistance to human defensins and evasion of neutrophil killing via the novel virulence factor MprF is based on modification of membrane lipids with l-lysine
    A Peschel
    Microbial Genetics, University of Tuebingen, 72076 Tuebingen, Germany
    J Exp Med 193:1067-76. 2001
    ..MprF thus constitutes a novel virulence factor, which may be of general relevance for bacterial pathogens and represents a new target for attacking multidrug resistant bacteria...
  5. ncbi Nucleotide sequence of the Staphylococcus aureus signal peptidase II (lsp) gene
    X J Zhao
    Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, MD 20899 4799
    FEBS Lett 299:80-4. 1992
    ..Unlike the lsp genes from the Gram-negative bacteria, the lsp gene in S. aureus is not flanked by x-ileS and orf149-orf316 as found in E. coli, Ent. aerogenes, and P. fluorescens...
  6. pmc DNA microarray-based identification of genes associated with glycopeptide resistance in Staphylococcus aureus
    Longzhu Cui
    Department of Bacteriology, Faculty of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Antimicrob Agents Chemother 49:3404-13. 2005
    ..We propose that the genes which raise glycopeptide resistance in S. aureus function toward altering the cell wall metabolic pathway...
  7. pmc Mgr, a novel global regulator in Staphylococcus aureus
    Thanh T Luong
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    J Bacteriol 185:3703-10. 2003
    ..Northern slot blot analyses suggested that mgr affected CP8, alpha-toxin, nuclease, and protein A at the transcriptional level...
  8. ncbi Three-dimensional structure of the complex between a T cell receptor beta chain and the superantigen staphylococcal enterotoxin B
    H Li
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville 20850, USA
    Immunity 9:807-16. 1998
    ..This variability can account for the preferential expression of certain V alpha regions among T cells reactive with SEB...
  9. pmc Intranasal exposure to bacterial superantigens induces airway inflammation in HLA class II transgenic mice
    Govindarajan Rajagopalan
    Department of Immunology, Mayo Clinic College of Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Infect Immun 74:1284-96. 2006
    ..In conclusion, repeated chronic intranasal exposure to bacterial superantigens causes airway inflammation and systemic immune activation...
  10. pmc A novel loop domain in superantigens extends their T cell receptor recognition site
    Sebastian Günther
    Boston Biomedical Research Institute, Watertown, MA 02472, USA
    J Mol Biol 371:210-21. 2007
    ..These interactions are absolutely required for TCR binding and T cell activation by SEK, and dictate the TCR Vbeta domain specificity of SEK and other group V SAGs...
  11. ncbi The agr P2 operon: an autocatalytic sensory transduction system in Staphylococcus aureus
    R P Novick
    Public Health Research Institute of the City of New York, Inc, NY 10016, USA
    Mol Gen Genet 248:446-58. 1995
    ..Since transcription of P2 requires P2 operon products, the P2 operon is autocatalytic, and is thus admirably suited to the need for rapid production of exoproteins at a time when overall growth is coming to a halt...
  12. pmc Role of VraSR in antibiotic resistance and antibiotic-induced stress response in Staphylococcus aureus
    S Gardete
    Molecular Genetics Laboratory, Instituto de Tecnologia Química e Biológica da Universidade Nova de Lisboa, Oeiras, Portugal
    Antimicrob Agents Chemother 50:3424-34. 2006
    ..One of the sites in cell wall synthesis "sensed" by the VraSR system appears to be a step catalyzed by PBP 2...
  13. pmc Different bacterial gene expression patterns and attenuated host immune responses are associated with the evolution of low-level vancomycin resistance during persistent methicillin-resistant Staphylococcus aureus bacteraemia
    Benjamin P Howden
    Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, Clayton, Victoria, Australia
    BMC Microbiol 8:39. 2008
    ..Based on the transcriptome results multiple genes were sequenced and innate immune system stimulation was assessed in the VSSA and hVISA/VISA pairs...
  14. pmc Identification, cloning, and initial characterization of rot, a locus encoding a regulator of virulence factor expression in Staphylococcus aureus
    P J McNamara
    Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA
    J Bacteriol 182:3197-203. 2000
    ..We named this ORF rot (for repressor of toxins) (GenBank accession no. AF189239) because of the activity associated with rot::Tn917 mutant strains...
  15. pmc Identification of a novel two-component regulatory system that acts in global regulation of virulence factors of Staphylococcus aureus
    J M Yarwood
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    J Bacteriol 183:1113-23. 2001
    ..Furthermore, srrAB may provide a mechanistic link between respiratory metabolism, environmental signals, and regulation of virulence factors in S. aureus...
  16. ncbi Identification and nucleotide sequence of the delta-lysin gene, hld, adjacent to the accessory gene regulator (agr) of Staphylococcus aureus
    L Janzon
    Department of Bacteriology, Karolinska Institutet, Stockholm, Sweden
    Mol Gen Genet 219:480-5. 1989
    ..Determination of hld mRNA half-life in different growth phases indicated that this regulation is at the level of transcription...
  17. pmc SarT, a repressor of alpha-hemolysin in Staphylococcus aureus
    K A Schmidt
    Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire 13755, USA
    Infect Immun 69:4749-58. 2001
    ..Collectively, these results indicated that the sarA locus, contrary to the regulatory action of agr, induced alpha-hemolysin production by repressing sarT, a repressor of hla transcription...
  18. ncbi Multiple mechanisms for the activation of human platelet aggregation by Staphylococcus aureus: roles for the clumping factors ClfA and ClfB, the serine-aspartate repeat protein SdrE and protein A
    Louise O'Brien
    Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland
    Mol Microbiol 44:1033-44. 2002
    ..aureus. Thus, S. aureus has multiple mechanisms for stimulating platelet aggregation. Such functional redundancy suggests that this phenomenon may be important in the pathogenesis of invasive diseases such as infective endocarditis...
  19. pmc Functional analysis of luxS in Staphylococcus aureus reveals a role in metabolism but not quorum sensing
    Neil Doherty
    Institute of Infections, Immunity, and Inflammation, University of Nottingham, Centre for Biomolecular Sciences, Nottingham NG7 2RD, United Kingdom
    J Bacteriol 188:2885-97. 2006
    ..aureus RN6390B during intracellular growth in epithelial cells: the wild type and a luxS mutant showed very similar growth patterns after their internalization by MAC-T cells...
  20. ncbi The two-component system ArlS-ArlR is a regulator of virulence gene expression in Staphylococcus aureus
    B Fournier
    Unité de Biochimie Microbienne, URA 2172 du Centre National de la Recherche Scientifique, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France
    Mol Microbiol 41:247-61. 2001
    ..Finally, arl was not autoregulated, but its expression was stimulated by agr and sarA. These results suggest that the Arl system interacts with both agr and sarA regulatory loci to modulate the virulence regulation network...
  21. pmc Sigma-B, a putative operon encoding alternate sigma factor of Staphylococcus aureus RNA polymerase: molecular cloning and DNA sequencing
    S Wu
    The Rockefeller University, New York, New York 10021, USA
    J Bacteriol 178:6036-42. 1996
    ..subtilis sigB operon. A Tn551 insertional mutant, RUSA168 (insert in orf256 of the staphylococcal sigma-B operon), showed drastic reduction in methicillin resistance (decrease in MIC from 1,600 microg ml-1 to 12 to 25 microg ml-1off..
  22. pmc Identification and molecular characterization of a putative regulatory locus that affects autolysis in Staphylococcus aureus
    E W Brunskill
    Program in Molecular and Cell Biology, University of Maryland, Baltimore County 21228, USA
    J Bacteriol 178:611-8. 1996
    ..These data suggest that the lytS and lytR gene products control the rate of autolysis in S. aureus by affecting the intrinsic murein hydrolase activity associated with the cell...
  23. pmc Cytoplasmic control of premature activation of a secreted protease zymogen: deletion of staphostatin B (SspC) in Staphylococcus aureus 8325-4 yields a profound pleiotropic phenotype
    Lindsey N Shaw
    Department of Biochemistry and Molecular Biology, University of Georgia, Life Sciences Bldg, Athens, GA 30602, USA
    J Bacteriol 187:1751-62. 2005
    ..Seemingly, some of these proteins may play a role in protein secretion; hence, their proteolytic inactivation by SspB has pleiotropic effects on the SspC-deficient mutant...
  24. pmc Regulation of exoprotein expression in Staphylococcus aureus by a locus (sar) distinct from agr
    A L Cheung
    Laboratory of Bacterial Pathogenesis and Immunology, Rockefeller University, New York, NY 10021
    Proc Natl Acad Sci U S A 89:6462-6. 1992
    ..aureus strain with a genetic background similar to strain 8325-4. This locus on the S. aureus chromosome, possibly regulatory in nature, has been designated sar for staphylococcal accessory regulator...
  25. doi Protecs, a comprehensive and powerful storage and analysis system for OMICS data, applied for profiling the anaerobiosis response of Staphylococcus aureus COL
    Stephan Fuchs
    Institute for Microbiology, Ernst Moritz Arndt University Greifswald, Greifswald, Germany
    Proteomics 10:2982-3000. 2010
    ..Interestingly, especially genes with a high codon adaptation index highly overlap with anaerobically induced genes...
  26. doi Proteolysis during long-term glucose starvation in Staphylococcus aureus COL
    Stephan Michalik
    Institute of Microbiology, Ernst Moritz Arndt University, D 17487 Greifswald, Germany
    Proteomics 9:4468-77. 2009
    ..and 2-D PAGE revealed that protein degradation appears to play a crucial role for the cell physiology of Staphylococcus aureus COL during extended periods of glucose starvation...
  27. pmc Comprehensive characterization of methicillin-resistant Staphylococcus aureus subsp. aureus COL secretome by two-dimensional liquid chromatography and mass spectrometry
    Shobha Ravipaty
    Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA
    Mol Cell Proteomics 9:1898-919. 2010
    ..protein and peptide mass spectrometry is used to characterize proteins secreted by methicillin-resistant Staphylococcus aureus COL. Protein identifications were accomplished via off-line protein fractionation followed by digestion and ..
  28. doi A protocol for the investigation of the intracellular Staphylococcus aureus metabolome
    Hanna Meyer
    Department of Pharmaceutical Biology, Friedrich Ludwig Jahn Street 15, 17489 Greifswald, Ernst Moritz Arndt University of Greifswald, Germany
    Anal Biochem 401:250-9. 2010
    ..Therefore, a protocol to elucidate the metabolome of the gram-positive pathogen, Staphylococcus aureus COL strain, grown in a chemically defined medium is introduced here...
  29. ncbi Haemin represses the haemolytic activity of Staphylococcus aureus in an Sae-dependent manner
    Julia Schmitt
    Institute of Medical Microbiology and Hygiene, University of Saarland Hospital, Homburg Saar, Germany
    Microbiology 158:2619-31. 2012
    ..aureus modulates its haemolysin production in response to iron and haem availability in an Sae-dependent manner...
  30. pmc Role of ArlRS in autolysis in methicillin-sensitive and methicillin-resistant Staphylococcus aureus strains
    Guido Memmi
    Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire, USA
    J Bacteriol 194:759-67. 2012
    ..Also, the autolytic phenotype in the arlRS mutant of MSSA strain Newman could be rescued by a mutation in either atl or lytM. Together, these data showed that ArlRS impacts autolysis differently in MSSA and MRSA strains...
  31. ncbi Expression of staphylococcal clumping factor A impedes macrophage phagocytosis
    Niklas Palmqvist
    Department of Rheumatology and Inflammation Research, Goteborg University, Guldhedsgatan 10 A, S 413 46 Goteborg, Sweden
    Microbes Infect 6:188-95. 2004
    ..Both the protection against phagocytosis and the enhanced immunostimulatory activity provided by ClfA expression are likely to contribute to the in vivo virulence of S. aureus...
  32. pmc An unusual isopentenyl diphosphate isomerase found in the mevalonate pathway gene cluster from Streptomyces sp. strain CL190
    K Kaneda
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 98:932-7. 2001
    ..aureus and of the different enzymatic properties of mammalian (type 1) and S. aureus (type 2) isomerases, this unusual enzyme is considered to be a suitable molecular target for the screening of antibacterial drugs specific to S. aureus...
  33. ncbi Structure and biosynthesis of staphyloxanthin from Staphylococcus aureus
    Alexandra Pelz
    Department of Microbial Genetics, University of Tuebingen, Germany
    J Biol Chem 280:32493-8. 2005
    ..Staphyloxanthin was identified as beta-D-glucopyranosyl 1-O-(4,4'-diaponeurosporen-4-oate)-6-O-(12-methyltetradecanoate)...
  34. pmc MsrR, a putative cell envelope-associated element involved in Staphylococcus aureus sarA attenuation
    Jutta Rossi
    Institute of Medical Microbiology, University of Zurich, CH 8028 Zurich, Switzerland
    Antimicrob Agents Chemother 47:2558-64. 2003
    ..These observations suggest that MsrR is a new component involved in sarA attenuation and the regulatory network controlling virulence gene expression in S. aureus...
  35. pmc Influence of a functional sigB operon on the global regulators sar and agr in Staphylococcus aureus
    M Bischoff
    Institute of Medical Microbiology, University of Zurich, CH 8028 Zurich, Switzerland
    J Bacteriol 183:5171-9. 2001
    ..The data presented here suggest that sigma(B) increases sar expression while simultaneously reducing the RNAIII level in a growth phase-dependent manner...
  36. ncbi Cloning and sequencing of the gene, fmtC, which affects oxacillin resistance in methicillin-resistant Staphylococcus aureus
    H Komatsuzawa
    Department of Microbiology, Hiroshima University School of Dentistry, Kasumi 1 2 3, Minami Ku, Hiroshima City, Hiroshima 734 8553, Japan
    FEMS Microbiol Lett 203:49-54. 2001
    ..9 kDa. The N-terminal half of the deduced FmtC protein is very hydrophobic, implying that this protein is a membrane-associated protein...
  37. ncbi Expression, purification, and characterization of peptidyl-tRNA hydrolase from Staphylococcus aureus
    Paul D Bonin
    Cell and Molecular Biology, Rockville, Maryland, USA
    Protein Expr Purif 24:123-30. 2002
    ..aureus Pth was determined to be 2.8 microM. A far UV circular dichroism spectrum revealed that His-tagged S. aureus Pth appears to have a structured core predominated by beta-sheet...
  38. pmc The N-acetylmannosamine transferase catalyzes the first committed step of teichoic acid assembly in Bacillus subtilis and Staphylococcus aureus
    Michael A D'Elia
    Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada
    J Bacteriol 191:4030-4. 2009
    ....
  39. ncbi Conformational movements and cooperativity upon amino acid, ATP and tRNA binding in threonyl-tRNA synthetase
    Alfredo Torres-Larios
    Laboratoire de Biologie et Genomique Structurales, IGBMC, 1 rue Laurent Fries, BP 10142, 67400 Illkirch Cedex, France
    J Mol Biol 331:201-11. 2003
    ..The structural analysis suggests that, while the small substrates can bind in any order, they must be in place before productive tRNA binding can occur...
  40. pmc Structures of the pleiotropic translational regulator Hfq and an Hfq-RNA complex: a bacterial Sm-like protein
    Maria A Schumacher
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, OR 97201 3098, USA
    EMBO J 21:3546-56. 2002
    ..Such binding suggests a mechanism for Hfq function...
  41. pmc A novel variant of the immunoglobulin fold in surface adhesins of Staphylococcus aureus: crystal structure of the fibrinogen-binding MSCRAMM, clumping factor A
    Champion C S Deivanayagam
    Center for Biophysical Sciences and Engineering, School of Optometry, 244 CBSE, 1025 18th Street South, University of Alabama at Birmingham, Birmingham, AL 35294 0005, USA
    EMBO J 21:6660-72. 2002
    ....
  42. pmc MprF-mediated lysinylation of phospholipids in Staphylococcus aureus leads to protection against oxygen-independent neutrophil killing
    Sascha A Kristian
    Microbial Genetics, University of Tubingen, 72076 Tubingen, Germany
    Infect Immun 71:546-9. 2003
    ..These results demonstrate the impact and specificity of MprF-mediated CAMP resistance and underscore the role of defensin-like peptides in innate host defense...
  43. ncbi Identification of temperature-sensitive dnaD mutants of Staphylococcus aureus that are defective in chromosomal DNA replication
    Y Li
    Laboratory of Developmental Biochemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 3 1, 7 chome, Hongo, Bunkyo ku, 113 0033 Tokyo, Japan
    Mol Genet Genomics 271:447-57. 2004
    ..Moreover, both mutants were sensitive to mitomycin C, a drug that induces DNA damage, suggesting that the DnaD protein is also involved in DNA repair...
  44. pmc SarA positively controls bap-dependent biofilm formation in Staphylococcus aureus
    Maria Pilar Trotonda
    Instituto Valenciano de Investigaciones Agrarias, Carretera Náquera Moncada Km 4, 5, 46113 Moncada, Valencia, Spain
    J Bacteriol 187:5790-8. 2005
    ..Based on the previous studies of others as well as our work demonstrating the role for SarA in icaADBC and bap expression, we propose that SarA is an essential regulator controlling biofilm formation in S. aureus...
  45. pmc Crystal structure of the SarS protein from Staphylococcus aureus
    Ronggui Li
    Integrated Department of Immunology, National Jewish Medical and Research Center, and Department of Pharmacology, Biomolecular Structure Program, School of Medicine, University of Colorado Health Science Center, Denver, Colorado 80206, USA
    J Bacteriol 185:4219-25. 2003
    ..Based on the structural differences of SarR, SarS, and MarR, we further classified these winged-helix proteins to three subfamilies, SarA, SarS, and MarR. Finally, a possible transcription regulation mechanism is proposed...
  46. ncbi A genetic system for analysis of staphylococcal nuclease
    D Shortle
    Gene 22:181-9. 1983
    ..In combination with present methods for efficient in vitro mutagenesis, this plasmid-based genetic system can be applied to the detailed genetic analysis of this small, biochemically and biophysically well-characterized enzyme...
  47. ncbi Functional expression of the alpha-hemolysin of Staphylococcus aureus in intact Escherichia coli and in cell lysates. Deletion of five C-terminal amino acids selectively impairs hemolytic activity
    B Walker
    Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545
    J Biol Chem 267:10902-9. 1992
    ..The mutant polypeptide nevertheless binds tightly to erythrocytes as a monomer, strengthening the idea that oligomerization is required for cell lysis...
  48. ncbi Chorismate synthase from Staphylococcus aureus
    M J Horsburgh
    Division of Biochemistry and Molecular Biology, University of Glasgow, UK
    Microbiology 142:2943-50. 1996
    ..The properties of S. aureus chorismate synthase are compared with those of the B. subtilis and E. coli enzymes...
  49. pmc Overproduction of a 37-kilodalton cytoplasmic protein homologous to NAD+-linked D-lactate dehydrogenase associated with vancomycin resistance in Staphylococcus aureus
    W M Milewski
    Department of Pediatrics, University of Chicago, Illinois 60637, USA
    Antimicrob Agents Chemother 40:166-72. 1996
    ..The increased D-lactate dehydrogenase activity in strain 523k and the structural similarities among Ddh, D-lactate dehydrogenase, and VanH suggest that overproduction of Ddh might play a role in vancomycin resistance in this strain...
  50. pmc Histidine residues near the N terminus of staphylococcal alpha-toxin as reporters of regions that are critical for oligomerization and pore formation
    R Jursch
    Institute of Medical Microbiology, University of Mainz, Germany
    Infect Immun 62:2249-56. 1994
    ..We interpret these results to indicate that the N-terminal domain of alpha-toxin in the region of H-35 and H-48 is involved in protomer-protomer interactions that underlie the hexamerization and pore-forming process...
  51. ncbi Cloning and primary structure of Staphylococcus aureus DNA topoisomerase IV: a primary target of fluoroquinolones
    L Ferrero
    Departement des Biotechnologies, Rhone Poulenc Rorer S A, Centre de Recherche de Vitry Alfortville, Vitry sur Seine, France
    Mol Microbiol 13:641-53. 1994
    ..coli GyrA, is substituted to Phe or Tyr in both high- and low-quinolone-resistant isolates. We propose that DNA topoisomerase IV is a primary target of fluoroquinolones in S. aureus...
  52. ncbi Identification of a chromosomally encoded ABC-transport system with which the staphylococcal erythromycin exporter MsrA may interact
    J I Ross
    Department of Microbiology, University of Leeds, UK
    Gene 153:93-8. 1995
    ..There was no further sequence similarity outside these conserved regions. These results indicate that the chromosomes of S. hominis and S. aureus contain sequences encoding a potential TM protein with which MsrA might interact...
  53. pmc A Staphylococcus aureus autolysin that has an N-acetylmuramoyl-L-alanine amidase domain and an endo-beta-N-acetylglucosaminidase domain: cloning, sequence analysis, and characterization
    T Oshida
    Rockefeller University, New York, NY 10021
    Proc Natl Acad Sci U S A 92:285-9. 1995
    ..aureus...
  54. ncbi Comparative biochemical and molecular analysis of the Staphylococcus hyicus, Staphylococcus aureus and a hybrid lipase. Indication for a C-terminal phospholipase domain
    K Nikoleit
    Universitat Tubingen, Germany
    Eur J Biochem 228:732-8. 1995
    ..hyicus lipase. The fact that two closely related enzymes differ in the need for Ca2+ underscores the notion that it plays a structural rather than a catalytic role...
  55. ncbi Identification of the active-site nucleophile in 6-phospho-beta-galactosidase from Staphylococcus aureus by labelling with synthetic inhibitors
    P Staedtler
    Arbeitsgruppe Physiologie der Mikroorganismen, Abteilung Biologie, Ruhr Universitat Bochum, Germany
    Eur J Biochem 232:658-63. 1995
    ..It was shown to be identical to an authentic, synthetic sample. From this, it is evident that E375 is the active-site nucleophile of 6-phospho-galactosidase, consistent with previous findings for enzymes in this family...
  56. ncbi A homologue to the Escherichia coli alkyl hydroperoxide reductase AhpC is induced by osmotic upshock in Staphylococcus aureus
    L Armstrong-Buisseret
    Department of Applied Biochemistry and Food Science, University of Nottingham, Faculty of Agricultural and Food Sciences, Leicestershire, UK
    Microbiology 141:1655-61. 1995
    ..Exposure of S. aureus to varying concentrations of H202 did not result in the detectable induction of AhpC...
  57. pmc Analysis of gyrA and grlA mutations in stepwise-selected ciprofloxacin-resistant mutants of Staphylococcus aureus
    L Ferrero
    Departement des Biotechnologies, Rhone Poulenc Rorer S A, Vitry sur Seine, France
    Antimicrob Agents Chemother 39:1554-8. 1995
    ..Ferrero, B. Cameron, B. Manse, D. Lagneaux, J. Crouzet, A. Famechon, and F. Blanche, Mol. Microbiol. 13:641-653, 1994) that DNA topoisomerase IV is a primary target of fluoroquinolones in S. aureus...
  58. pmc Primary sequence of the alpha-toxin gene from Staphylococcus aureus wood 46
    G S Gray
    Infect Immun 46:615-8. 1984
    ..The mature alpha-toxin protein had a molecular size of 33,000 and contained only three short regions of high hydrophobicity in addition to a number of short, weakly hydrophobic regions...
  59. ncbi Amino acid sequence of the amphiphilic phosphocarrier protein factor IIILac of the lactose-specific phosphotransferase system of Staphylococcus
    K Stuber
    Biochemistry 24:1164-8. 1985
    ..The N-terminal part of the protein comprising approximately one-third of the chain exhibits in vitro affinity toward membrane-bound enzyme IILac...
  60. ncbi The phosphoenolpyruvate:sugar phosphotransferase system in gram-positive bacteria: properties, mechanism, and regulation
    J Reizer
    Department of Biology, University of California at San Diego, La Jolla
    Crit Rev Microbiol 15:297-338. 1988
    ....
  61. ncbi Nucleotide sequence analysis of the gene for protein A from Staphylococcus aureus Cowan 1 (NCTC8530) and its enhanced expression in Escherichia coli
    H L Shuttleworth
    Microbial Technology Laboratory, PHLS, Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wilts, U K
    Gene 58:283-95. 1987
    ..The homology to the latter probably identifies the Fc-binding region in the immunoglobulin-binding domains of SPA. Enhanced production of SPA in Escherichia coli was achieved using the lac promoter immediately upstream from the spa gene...
  62. ncbi The coagulase of Staphylococcus aureus 8325-4. Sequence analysis and virulence of site-specific coagulase-deficient mutants
    P Phonimdaeng
    Department of Microbiology, Moyne Institute, Trinity College, Dublin, Ireland
    Mol Microbiol 4:393-404. 1990
    ..This contradicts findings of several groups using Coa- mutants generated by chemical mutagenesis and suggests that the earlier results were obtained with strains that had suffered additional mutations in virulence-related genes...
  63. pmc Complete nucleotide sequence of IS431mec in Staphylococcus aureus
    L Barberis-Maino
    Institute of Medical Microbiology, University of Zurich, Switzerland
    Nucleic Acids Res 18:5548. 1990
  64. pmc Localized perforation of the cell wall by a major autolysin: atl gene products and the onset of penicillin-induced lysis of Staphylococcus aureus
    M Sugai
    Department of Microbiology, Hiroshima University School of Dentistry, Japan
    J Bacteriol 179:2958-62. 1997
    ....
  65. ncbi Glutamine synthetase and heteroresistance in methicillin-resistant Staphylococcus aureus
    A M Stranden
    Institute of Medical Microbiology, University of Zurich, Switzerland
    Microb Drug Resist 2:201-7. 1996
    ..This suggests that S. aureus can adopt multiple ways to achieve high methicillin resistance...
  66. ncbi Antiseptic susceptibility and distribution of antiseptic-resistance genes in methicillin-resistant Staphylococcus aureus
    N Noguchi
    Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan
    FEMS Microbiol Lett 172:247-53. 1999
    ..Our results indicated that four or more antiseptic-resistance genes exist in methicillin-resistant S. aureus and that antiseptic-resistant methicillin-resistant S. aureus strains without qacA and smr are widely spread in Japan...
  67. ncbi Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall
    S K Mazmanian
    Department of Microbiology and Immunology, UCLA School of Medicine, University of California, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA
    Science 285:760-3. 1999
    ..The protein specified by srtA, sortase, may be a useful target for the development of new antimicrobial drugs...
  68. ncbi Topological analysis of the MraY protein catalysing the first membrane step of peptidoglycan synthesis
    A Bouhss
    Biochimie Structurale et Cellulaire, CNRS, Batiment 430, Universite Paris Sud, F 91405, Orsay, France
    Mol Microbiol 34:576-85. 1999
    ..It was noteworthy that S. aureus MraY was functional in E. coli. An increased MraY transferase activity was observed only with the untruncated hybrids from both organisms...
  69. ncbi Antibiotic resistance as a stress response: complete sequencing of a large number of chromosomal loci in Staphylococcus aureus strain COL that impact on the expression of resistance to methicillin
    H de Lencastre
    Laboratory of Microbiology, The Rockefeller University, New York, NY 10021, USA
    Microb Drug Resist 5:163-75. 1999
    ..Sequencing upstream and downstream allowed the identification of a number of additional open reading frames, some of which may also include functions relevant for the expression of antibiotic resistance...
  70. pmc Autophosphorylation of phosphoglucosamine mutase from Escherichia coli
    L Jolly
    Laboratoire des Enveloppes Bactériennes et Antibiotiques, Centre National de la Recherche Scientifique, Universite Paris Sud, 91405 Orsay, France
    J Bacteriol 182:1280-5. 2000
    ..coli GlmM...
  71. ncbi Clumping factor B (ClfB), a new surface-located fibrinogen-binding adhesin of Staphylococcus aureus
    D Ni Eidhin
    Microbiology Department, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland
    Mol Microbiol 30:245-57. 1998
    ....
  72. ncbi Molecular analysis of the accessory gene regulator (agr) locus and balance of virulence factor expression in epidemic methicillin-resistant Staphylococcus aureus
    H Papakyriacou
    Department of Microbiology, Sunnybrook and Women s College Health Science Centre, University of Toronto, Toronto, Ontario, Canada
    J Infect Dis 181:990-1000. 2000
    ..Thus, the balance of virulence factor expression in CMRSA-1 and CMRSA-3 favors the colonization phase of infection, and CMRSA-1 possesses unique genotypic and phenotypic traits...
  73. pmc Identification and characterization of a membrane permease involved in iron-hydroxamate transport in Staphylococcus aureus
    M T Sebulsky
    Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada, N6A 5C1
    J Bacteriol 182:4394-400. 2000
    ..By using the Fur titration assay, it was shown that the Fur box sequences upstream of fhuCBG are recognized by the Escherichia coli Fur protein...
  74. ncbi Characterization of a novel structural member, LukE-LukD, of the bi-component staphylococcal leucotoxins family
    A Gravet
    UPRES EA 1318, Institut de Bacteriologie de la Faculte de Medecine, Université Louis Pasteur Hôpitaux Universitaires de Strasbourg, France
    FEBS Lett 436:202-8. 1998
    ..005). LukE+LukD was as effective as the Panton-Valentine leucocidin for inducing dermonecrosis when injected in the rabbit skin, but not hemolytic and poorly leucotoxic compared to other leucotoxins expressed by Staphylococcus aureus...
  75. pmc Genetic instability of the global regulator agr explains the phenotype of the xpr mutation in Staphylococcus aureus KSI9051
    P J McNamara
    Department of Microbiology and Immunology, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma 73190, USA
    J Bacteriol 180:2609-15. 1998
    ..A comparison of the nucleotide sequences of AgrC from several strains revealed seven errors in the GenBank entry for agr (X52543); these data were confirmed with plasmid pRN6650, the original wild-type clone of agr...
  76. pmc Identification and characterization of SirA, an iron-regulated protein from Staphylococcus aureus
    J H Heinrichs
    MedImmune, Inc, Gaithersburg, Maryland 20878, USA
    J Bacteriol 181:1436-43. 1999
    ..aureus culture medium. sir-encoded proteins may be involved in iron acquisition in vivo and therefore may be targets for antimicrobial agents...
  77. pmc Antibacterial action of extracellular mammalian group IIA phospholipase A2 against grossly clumped Staphylococcus aureus
    M E Dominiecki
    Department of Microbiology, New York University School of Medicine, New York, New York 10016, USA
    Infect Immun 67:2299-305. 1999
    ..Thus, the extracellular mobilization of group IIA PLA2 during inflammation may provide a mechanism by which the host can control the proliferation and survival of S. aureus even after bacterial clumping...
  78. pmc Molecular basis for the role of Staphylococcus aureus penicillin binding protein 4 in antimicrobial resistance
    Vikas Navratna
    Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India
    J Bacteriol 192:134-44. 2010
    ..Put together, the expression analysis and biochemical features of PBP4 provide a framework for understanding the function of this protein in S. aureus and its role in antimicrobial resistance...
  79. doi A nitric oxide-inducible lactate dehydrogenase enables Staphylococcus aureus to resist innate immunity
    Anthony R Richardson
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
    Science 319:1672-6. 2008
    ..NO.-inducible lactate dehydrogenase activity and NO. resistance distinguish S. aureus from the closely related commensal species S. epidermidis and S. saprophyticus...
  80. pmc A general method for cloning recA genes of gram-positive bacteria by polymerase chain reaction
    P Duwat
    Laboratoire de Genetique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France
    J Bacteriol 174:5171-5. 1992
    ..This method is particularly useful for the recovery of the recA genes of gram-positive bacteria and avoids the difficulties of using a genetic complementation test for cloning...
  81. pmc Influence of the two-component system SaeRS on global gene expression in two different Staphylococcus aureus strains
    Kathrin Rogasch
    Institut fur Mikrobiologie, Ernst Moritz Arndt Universitat, F L Jahn Strasse 15, D 17487 Greifswald, Germany
    J Bacteriol 188:7742-58. 2006
    ..Our results clearly show that SaeRS is strongly involved in the tight temporal control of virulence factor expression in S. aureus. Its precise role within the regulatory network remains to be determined...
  82. ncbi Comparison of the beta-toxins from Staphylococcus aureus and Staphylococcus intermedius
    K Dziewanowska
    Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow 83844 3052, USA
    Arch Biochem Biophys 335:102-8. 1996
    ..Further evidence that the two proteins differ was obtained by immunological analysis which demonstrated crossreactivity but a lack of identity...
  83. pmc Characterization of NorR protein, a multifunctional regulator of norA expression in Staphylococcus aureus
    Que Chi Truong-Bolduc
    Division of Infectious Diseases and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114 2696, USA
    J Bacteriol 185:3127-38. 2003
    ..Collectively, these results indicate that NorR is a multifunctional regulator, affecting cell surface properties as well as the expression of NorA and likely other multidrug resistance efflux pumps...
  84. pmc Regulatory elements of the Staphylococcus aureus protein A (Spa) promoter
    Jinxin Gao
    Department of Diagnostic Medicine Pathobiology, College of Veterinary Medicine, Kansas State University, 1800 Denison Ave, Manhattan, KS 66506, USA
    J Bacteriol 186:3738-48. 2004
    ..Full repression required the presence of a second consensus site adjacent to the SarS binding site. Sequences directly upstream of the core promoter sequence were found to stimulate transcription...
  85. pmc Reduced content of lysyl-phosphatidylglycerol in the cytoplasmic membrane affects susceptibility to moenomycin, as well as vancomycin, gentamicin, and antimicrobial peptides, in Staphylococcus aureus
    Hiromi Nishi
    Department of Bacteriology, Hiroshima University Graduate School of Biomedical Sciences, Kasumi 1 2 3, Minami Ku, Hiroshima City, Hiroshima, Japan
    Antimicrob Agents Chemother 48:4800-7. 2004
    ..Therefore, the membrane charge of S. aureus appears to influence the efficacies of moenomycin, vancomycin, and other cationic antimicrobial agents...
  86. ncbi A genetic and molecular characterization of the recA gene from Staphylococcus aureus
    K W Bayles
    Department of Biological Sciences, University of Maryland, Baltimore 21228
    Gene 147:13-20. 1994
    ..Furthermore, genetic and physical mapping of S. aureus recA placed it in the same region as the uvs-568 mutation. These data strongly suggest that these mutations represent different alleles of the same recA gene...
  87. ncbi Staphylococcus aureus clumping factor B (ClfB) promotes adherence to human type I cytokeratin 10: implications for nasal colonization
    Louise M O'Brien
    Moyne Institute of Preventive Medicine, Department of Microbiology, Trinity College Dublin, Dublin 2, Ireland
    Cell Microbiol 4:759-70. 2002
    ..We also showed that ClfB is transcribed by S. aureus in the human nares. We propose that ClfB is a major determinant in S. aureus nasal colonization...
  88. pmc Transcriptional analysis of the Staphylococcus aureus penicillin binding protein 2 gene
    M G Pinho
    Laboratory of Microbiology, The Rockefeller University, New York, New York 10021, USA
    J Bacteriol 180:6077-81. 1998
    ..We now show that introduction of the intact pbp2 gene with its two newly identified promoters into the chromosome of the transposon mutant resulted in the full recovery of high-level methicillin resistance...
  89. pmc Successful multiresistant community-associated methicillin-resistant Staphylococcus aureus lineage from Taipei, Taiwan, that carries either the novel Staphylococcal chromosome cassette mec (SCCmec) type VT or SCCmec type IV
    Susan Boyle-Vavra
    Department of Pediatrics, Section of Infectious Diseases, University of Chicago, IL, 60637, USA
    J Clin Microbiol 43:4719-30. 2005
    ..SCCmec V(T) is a novel SCCmec variant that is found in multiply resistant CAMRSA strains with sequence type 59 in Taipei in association with the PVL leukotoxin genes...
  90. ncbi Modification of autolysis by synthetic peptides derived from the presumptive binding domain of Staphylococcus aureus autolysin
    M Takano
    Pharmaceutical Development Research Laboratory, Tanabe Seiyaku Co, Ltd, Saitama, Japan
    Microbiol Immunol 44:463-72. 2000
    ..aureus autolysin activities. These peptides, especially the 10-mer peptide B9 (PGTKLYTVPW) that represents the C-terminal half of A10 and N-terminal half of A11, may be important segments for Atl to bind to the cell surface...
  91. pmc Novel type of staphylococcal cassette chromosome mec identified in community-acquired methicillin-resistant Staphylococcus aureus strains
    Xiao Xue Ma
    Department of Bacteriology, Juntendo University, Tokyo, Japan
    Antimicrob Agents Chemother 46:1147-52. 2002
    ..Consistent with the strains' susceptibilities to various non-beta-lactam antibiotics, the type IV SCCmec was devoid of any antibiotic resistance genes other than the mecA gene...

Research Grants2

  1. Gordon Research Conference on Staphylococcal Diseases
    BRIGITTE BERGER BACHI; Fiscal Year: 2003
    ..S. This will afford a unique and rich opportunity for fellowship and scientific exchange between young and established investigators in the field. ..
  2. Characterization of ??S in the stress & virulence responses of S. aureus
    LINDSEY NEIL SHAW; Fiscal Year: 2010
    ..This proposal seeks to investigate and understand how a novel regulator of virulence, CS, contributes to disease causation in S. aureus. ..