Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842


Alias: Lactobacillus delbrueckii subsp. bulgaricus DSM 20081, Lactobacillus delbrueckii subsp. bulgaricus NCDO 1489, Lactobacillus delbrueckii subsp. bulgaricus str. ATCC 11842, Lactobacillus delbrueckii subsp. bulgaricus strain ATCC 11842

Top Publications

  1. Zhai Z, Douillard F, An H, Wang G, Guo X, Luo Y, et al. Proteomic characterization of the acid tolerance response in Lactobacillus delbrueckii subsp. bulgaricus?CAUH1 and functional identification of a novel acid stress-related transcriptional regulator Ldb0677. Environ Microbiol. 2014;16:1524-37 pubmed publisher
    ..The identification of a binding motif (SSTAGACR) present in the promoter regions of 22 genes indicates that it might function as a major regulator in acid stress response in L.?bulgaricus. ..
  2. Ravin V, Alatossava T. A new insertion sequence element, ISLdl1, in Lactobacillus delbrueckii subsp. lactis ATCC 15808. Microbiol Res. 2002;157:109-14 pubmed
    ..ISLdl1 or ISLdl1-like IS elements were found exclusively in Lactobacillus delbrueckii species and in all strains of subsp. lactis tested. The nucleotide sequence of ISLdl1 is deposited under the accession number AJ302652. ..
  3. Bernard N, Johnsen K, Gelpi J, Alvarez J, Ferain T, Garmyn D, et al. D-2-hydroxy-4-methylvalerate dehydrogenase from Lactobacillus delbrueckii subsp. bulgaricus. II. Mutagenic analysis of catalytically important residues. Eur J Biochem. 1997;244:213-9 pubmed
    ..5. ..
  4. Ishino Y, Morgenthaler P, Hottinger H, Soll D. Organization and nucleotide sequence of the glutamine synthetase (glnA) gene from Lactobacillus delbrueckii subsp. bulgaricus. Appl Environ Microbiol. 1992;58:3165-9 pubmed
    ..The lack of a glnR gene in the L. delbrueckii subsp. bulgaricus DNA in this position may indicate a gene rearrangement or a different mechanism of glnA gene expression. ..
  5. Vongerichten K, Klein J, Matern H, Plapp R. Cloning and nucleotide sequence analysis of pepV, a carnosinase gene from Lactobacillus delbrueckii subsp. lactis DSM 7290, and partial characterization of the enzyme. Microbiology. 1994;140 ( Pt 10):2591-600 pubmed
    ..A second ORF present in the sequenced fragment showed extensive homology to a variety of amino acid permeases from E. coli and Saccharomyces cerevisiae. ..
  6. Branny P, de la Torre F, Garel J. The genes for phosphofructokinase and pyruvate kinase of Lactobacillus delbrueckii subsp. bulgaricus constitute an operon. J Bacteriol. 1996;178:4727-30 pubmed
    ..9-kb RNA. The nucleotide sequence of the pyk gene indicated that the encoded protein possessed an extra C-terminal domain with a potential phosphoenolpyruvate-dependent autophosphorylation site. ..
  7. Bernard N, Johnsen K, Ferain T, Garmyn D, Hols P, Holbrook J, et al. NAD(+)-dependent D-2-hydroxyisocaproate dehydrogenase of Lactobacillus delbrueckii subsp. bulgaricus. Gene cloning and enzyme characterization. Eur J Biochem. 1994;224:439-46 pubmed
    ..The L. delbrueckii subsp. bulgaricus enzyme, like that of L. casei, was shown to be active on a wide variety of 2-oxoacid substrates except those having a branched beta-carbon. ..
  8. Meyer Barton E, Klein J, Imam M, Plapp R. Cloning and sequence analysis of the X-prolyl-dipeptidyl-aminopeptidase gene (pepX) from Lactobacillus delbrückii ssp. lactis DSM7290. Appl Microbiol Biotechnol. 1993;40:82-9 pubmed
    ..Nucleotide sequence analysis revealed an open reading frame of 2376 bp, coding for a protein of 792 amino acids with a molecular mass of 88449 Da. ..
  9. Ulrich R, Hughes T. Cloning and expression analysis of the 28 kDa protein from Lactobacillus delbrueckii subsp. lactis ATCC 4797 hypothesized to influence lactacin B production. J Appl Microbiol. 2001;91:1067-73 pubmed
    ..1994) hypothesizing that a purified IP from Lact. lactis, homologous to a Bacillus stearothermophilus Tpi, is capable of enhancing bacteriocin synthesis in Lact. acidophilus N2. ..

More Information


  1. Ravin V, Alatossava T. Three new insertion sequence elements ISLdl2, ISLdl3, and ISLdl4 in Lactobacillus delbrueckii: isolation, molecular characterization, and potential use for strain identification. Plasmid. 2003;49:253-68 pubmed
    ..We demonstrate for the first time that inverse PCR and vectorette PCR methods with primers based on sequences of the IS elements could be used for identification of L. delbrueckii strains. ..
  2. Aubel D, Germond J, Gilbert C, Atlan D. Isolation of the patC gene encoding the cystathionine beta-lyase of Lactobacillus delbrueckii subsp. bulgaricus and molecular analysis of inter-strain variability in enzyme biosynthesis. Microbiology. 2002;148:2029-36 pubmed
    ..Moreover, the results of this study suggest that post-transcriptional mechanisms account for the differences in CBL activities observed between strains of Lb. delbrueckii. ..
  3. Klein J, Schick J, Henrich B, Plapp R. Lactobacillus delbrueckii subsp. lactis DSM7290 pepG gene encodes a novel cysteine aminopeptidase. Microbiology. 1997;143 ( Pt 2):527-37 pubmed
    ..Primer extension analysis, using mRNA isolated from Lb. delbrueckii subsp. lactis DSM7290 did establish that orfW was transcribed. ..
  4. Klein J, Henrich B, Plapp R. Cloning and nucleotide sequence analysis of the Lactobacillus delbrueckii ssp. lactis DSM7290 cysteine aminopeptidase gene pepC. FEMS Microbiol Lett. 1994;124:291-9 pubmed
    ..The predicted open reading frame consists of 449 codons, coding for a protein of 50,909 Da. The enzyme is functional and extremely overexpressed in E. coli. ..
  5. Kochhar S, Chuard N, Hottinger H. Cloning and overexpression of the Lactobacillus bulgaricus NAD(+)-dependent D-lactate dehydrogenase gene in Escherichia coli:purification and characterization of the recombinant enzyme. Biochem Biophys Res Commun. 1992;185:705-12 pubmed
    ..All the properties of the recombinant protein, e.g., optimum pH and temperature, Km and k(cat) for pyruvate as well as for other 2-oxo acids and the subunit structure were identical to the wild-type enzyme. ..
  6. Schenk Gröninger R, Becker J, Brendel M. Cloning, sequencing, and characterizing the Lactobacillus leichmannii pyrC gene encoding dihydroorotase. Biochimie. 1995;77:265-72 pubmed
    ..6% identity with the corresponding Bacillus subtilis enzyme. Highly conserved protein domains suggest importance for the enzyme's function. ..
  7. Leong Morgenthaler P, Oliver S, Hottinger H, Soll D. A Lactobacillus nifS-like gene suppresses an Escherichia coli transaminase B mutation. Biochimie. 1994;76:45-9 pubmed
    ..We show that the L bulgaricus NifS-like protein is able to replace in vivo transaminase B in E coli. This experimental observation supports the prediction that some NifS-like proteins may be aminotransferases. ..
  8. Klein J, Klein U, Schad M, Plapp R. Cloning, DNA sequence analysis and partial characterization of pepN, a lysyl aminopeptidase from Lactobacillus delbrückii ssp. lactis DSM7290. Eur J Biochem. 1993;217:105-14 pubmed
    ..In a four-step procedure including streptomycin sulfate precipitation, anion-exchange chromatography and gel filtration the peptidase was purified to electrophoretic homogeneity. ..
  9. Weber B, Klein J, Henrich B. The arbZ gene from Lactobacillus delbrueckii subsp. lactis confers to Escherichia coli the ability to utilize the beta-glucoside arbutin. Gene. 1998;212:203-11 pubmed
    ..In Lb. delbrueckii subsp. lactis DSM7290 two transcripts, one covering arbX together with arbZ and one covering arbZ alone were detected by Northern blot analysis. ..
  10. Li C, Sun J, Zhang G, Liu L. Effect of the absence of the CcpA gene on growth, metabolic production, and stress tolerance in Lactobacillus delbrueckii ssp. bulgaricus. J Dairy Sci. 2016;99:104-11 pubmed publisher
    ..bulgaricus is constructed and the effect of aerobic growth on stress tolerance of L. bulgaricus is evaluated. Although aerobic cultivation does not significantly improve growth, it does improve stress tolerance. ..
  11. Stucky K, Schick J, Klein J, Henrich B, Plapp R. Characterization of pepR1, a gene coding for a potential transcriptional regulator of Lactobacillus delbrueckii subsp. lactis DSM7290. FEMS Microbiol Lett. 1996;136:63-9 pubmed
    ..The pepQ-beta-galactosidase hybrid displayed an enhanced expression in the presence of cloned pepR1. ..
  12. Le Bras G, Deville Bonne D, Garel J. Purification and properties of the phosphofructokinase from Lactobacillus bulgaricus. A non-allosteric analog of the enzyme from Escherichia coli. Eur J Biochem. 1991;198:683-7 pubmed
  13. Schmidt B, Adams R, Requadt C, Power S, Mainzer S. Expression and nucleotide sequence of the Lactobacillus bulgaricus beta-galactosidase gene cloned in Escherichia coli. J Bacteriol. 1989;171:625-35 pubmed
    ..However, these enzymes are quite different from the thermophilic beta-galactosidase encoded by the Bacillus stearothermophilus bgaB gene...
  14. Kochhar S, Hunziker P, Leong Morgenthaler P, Hottinger H. Evolutionary relationship of NAD(+)-dependent D-lactate dehydrogenase: comparison of primary structure of 2-hydroxy acid dehydrogenases. Biochem Biophys Res Commun. 1992;184:60-6 pubmed
    ..The data suggest that L- and D-specific 2-hydroxy acid dehydrogenase genes evolved from two different ancestors and thus represent two different sets of enzyme families...
  15. Paricharttanakul N, Ye S, Menefee A, Javid Majd F, Sacchettini J, Reinhart G. Kinetic and structural characterization of phosphofructokinase from Lactobacillus bulgaricus. Biochemistry. 2005;44:15280-6 pubmed publisher
    ..The effector binding site, however, shows many differences that could explain the observed decreases in binding affinity for MgADP and PEP in LbPFK as compared to the other two enzymes...
  16. Leong Morgenthaler P, Zwahlen M, Hottinger H. Lactose metabolism in Lactobacillus bulgaricus: analysis of the primary structure and expression of the genes involved. J Bacteriol. 1991;173:1951-7 pubmed
    ..coli, but the residues which are involved in the binding and the transport of lactose are conserved. The carboxy terminus is similar to that of the enzyme III of several phosphoenolpyruvate-dependent phosphotransferase systems...
  17. Razeto A, Kochhar S, Hottinger H, Dauter M, Wilson K, Lamzin V. Domain closure, substrate specificity and catalysis of D-lactate dehydrogenase from Lactobacillus bulgaricus. J Mol Biol. 2002;318:109-19 pubmed publisher
    ..In pyruvate reduction, D-LDH can adapt another protonated residue, a lysine residue, to accomplish the role of the acid catalyst His296. Required lowering of the lysine pK(a) value is explained on the basis of the H296K mutant structure...
  18. Peltoniemi K, Vesanto E, Palva A. Genetic characterization of an oligopeptide transport system from Lactobacillus delbrueckii subsp. bulgaricus. Arch Microbiol. 2002;177:457-67 pubmed publisher
    ..Only the L. delbrueckii subsp. bulgaricus oppDFBCA (1)genes were able to complement the L. lactis oppA mutation...
  19. Bernard N, Ferain T, Garmyn D, Hols P, Delcour J. Cloning of the D-lactate dehydrogenase gene from Lactobacillus delbrueckii subsp. bulgaricus by complementation in Escherichia coli. FEBS Lett. 1991;290:61-4 pubmed
    ..The open reading frame encoded a 333-amino acid protein, showing no similarity with known L-LDH sequences but closely related to L. casei D-hydroxyisocaproate dehydrogenase (D-HicDH)...
  20. Sintchak M, Arjara G, Kellogg B, Stubbe J, Drennan C. The crystal structure of class II ribonucleotide reductase reveals how an allosterically regulated monomer mimics a dimer. Nat Struct Biol. 2002;9:293-300 pubmed publisher
    ..Thus, L. leichmannii RNR is a paradigm for the simplest structural entity capable of ribonucleotide reduction, a reaction linking the RNA and DNA worlds...
  21. Cook W, Short S, Ealick S. Crystallization and preliminary X-ray investigation of recombinant Lactobacillus leichmannii nucleoside deoxyribosyltransferase. J Biol Chem. 1990;265:2682-3 pubmed
    ..8-A resolution. It appears that the molecule, which is a hexamer, utilizes the symmetry of the space group, resulting in two or three subunits per asymmetric unit...
  22. Branny P, de la Torre F, Garel J. An operon encoding three glycolytic enzymes in Lactobacillus delbrueckii subsp. bulgaricus: glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase and triosephosphate isomerase. Microbiology. 1998;144 ( Pt 4):905-14 pubmed publisher
  23. Lin A, Ashley G, Stubbe J. Location of the redox-active thiols of ribonucleotide reductase: sequence similarity between the Escherichia coli and Lactobacillus leichmannii enzymes. Biochemistry. 1987;26:6905-9 pubmed
    ..4 equiv of 14C is located on the two cysteines of C-E-G-G-A-C-P-I-K. This peptide shows remarkable and unexpected similarity to the thiol-containing region of the C-terminal peptide of E. coli B1, C-E-S-G-A-C-K-I...
  24. Atlan D, Gilbert C, Blanc B, Portalier R. Cloning, sequencing and characterization of the pepIP gene encoding a proline iminopeptidase from Lactobacillus delbrueckii subsp. bulgaricus CNRZ 397. Microbiology. 1994;140 ( Pt 3):527-35 pubmed publisher
    ..The PepIP opens a new way for supplying cells with proline using the peptides resulting from the proteolytic degradation of caseins...
  25. Armstrong S, Cook W, Short S, Ealick S. Crystal structures of nucleoside 2-deoxyribosyltransferase in native and ligand-bound forms reveal architecture of the active site. Structure. 1996;4:97-107 pubmed
    ..A comparison of the enzyme interactions with both a purine and pyrimidine ligand provides some insight into the structural basis for enzyme specificity...
  26. Booker S, Stubbe J. Cloning, sequencing, and expression of the adenosylcobalamin-dependent ribonucleotide reductase from Lactobacillus leichmannii. Proc Natl Acad Sci U S A. 1993;90:8352-6 pubmed
    ..5 g of bacteria. Initial characterization of the recombinant RTPR indicates that its properties are identical to those of the RTPR isolated from L. leichmannii...
  27. Kim S, Nalaskowska M, Germond J, Pridmore D, S ll D. Asn-tRNA in Lactobacillus bulgaricus is formed by asparaginylation of tRNA and not by transamidation of Asp-tRNA. Nucleic Acids Res. 1996;24:2648-51 pubmed
    ..This indicates that L.bulgaricus contains a functional AsnRS. Thus, the transamidation pathway operates only for Gin-tRNAGln formation in this organism, and possibly in all gram-positive eubacteria...
  28. Gilbert C, Atlan D, Blanc B, Portalier R. Proline iminopeptidase from Lactobacillus delbrueckii subsp. bulgaricus CNRZ 397: purification and characterization. Microbiology. 1994;140 ( Pt 3):537-42 pubmed publisher
    ..The NH2-terminal amino acid sequence of the purified PepIP corresponds to the amino acid sequence deduced from the nucleotide sequence of the pepIP gene...
  29. Vinals C, Depiereux E, Feytmans E. Prediction of structurally conserved regions of D-specific hydroxy acid dehydrogenases by multiple alignment with formate dehydrogenase. Biochem Biophys Res Commun. 1993;192:182-8 pubmed publisher
  30. Branny P, de la Torre F, Garel J. Cloning, sequencing, and expression in Escherichia coli of the gene coding for phosphofructokinase in Lactobacillus bulgaricus. J Bacteriol. 1993;175:5344-9 pubmed
    ..bulgaricus, the coordinated regulation of phosphofructokinase and pyruvate kinase occurs at the levels of both biosynthesis and enzymatic activity...
  31. Porter D, Merrill B, Short S. Identification of the active site nucleophile in nucleoside 2-deoxyribosyltransferase as glutamic acid 98. J Biol Chem. 1995;270:15551-6 pubmed
    ..Collectively, chemical modification and mutagenesis studies have identified Glu-98 as the active site nucleophile of nucleoside 2-deoxyribosyltransferase...
  32. Kochhar S, Hunziker P, Leong Morgenthaler P, Hottinger H. Primary structure, physicochemical properties, and chemical modification of NAD(+)-dependent D-lactate dehydrogenase. Evidence for the presence of Arg-235, His-303, Tyr-101, and Trp-19 at or near the active site. J Biol Chem. 1992;267:8499-513 pubmed
    ..Arg-235 and His-303 are involved in the binding of 2-oxo acid substrate whereas other residues are involved in binding of the cofactor...
  33. Serror P, Dervyn R, Ehrlich S, Maguin E. csp-like genes of Lactobacillus delbrueckii ssp. bulgaricus and their response to cold shock. FEMS Microbiol Lett. 2003;226:323-30 pubmed
    ..In contrast, cspB is part of an operon transcribed at constant level irrespective of the temperature. These results indicate that cspA encodes the only Csp-like protein of L. bulgaricus induced by a downshift of temperature...
  34. Germond J, Lapierre L, Delley M, Mollet B, Felis G, Dellaglio F. Evolution of the bacterial species Lactobacillus delbrueckii: a partial genomic study with reflections on prokaryotic species concept. Mol Biol Evol. 2003;20:93-104 pubmed publisher
    ..This study has consequences on the prokaryotic species concept, since genomic flexibility of prokaryotes collides with a stable classification, necessary from a scientific and applied point of view...
  35. Jozic D, Bourenkow G, Bartunik H, Scholze H, Dive V, Henrich B, et al. Crystal structure of the dinuclear zinc aminopeptidase PepV from Lactobacillus delbrueckii unravels its preference for dipeptides. Structure. 2002;10:1097-106 pubmed
    ..The cocrystallized inhibitor illustrates the two roles of the two catalytic zinc ions, namely stabilization of the tetrahedral intermediate and activation of the catalytic water molecule...
  36. Stucky K, Hagting A, Klein J, Matern H, Henrich B, Konings W, et al. Cloning and characterization of brnQ, a gene encoding a low-affinity, branched-chain amino acid carrier in Lactobacillus delbrückii subsp. lactis DSM7290. Mol Gen Genet. 1995;249:682-90 pubmed
    ..lactis DSM7290, the amino acid sequence of its product and the topology of the hydrophobic domains with those of the respective carrier genes and proteins of Salmonella typhimurium and Pseudomonas aeruginosa revealed extensive homology...
  37. Klein J, Schmidt U, Plapp R. Cloning, heterologous expression, and sequencing of a novel proline iminopeptidase gene, pepI, from Lactobacillus delbrueckii subsp. lactis DSM 7290. Microbiology. 1994;140 ( Pt 5):1133-9 pubmed publisher
    ..Amino acid homology to the active site of a Pseudomonas putida esterase and inhibitor studies of the enzyme imply involvement of a serine residue in catalysis...
  38. Kim S, Germond J, Pridmore D, S ll D. Lactobacillus bulgaricus asparagine synthetase and asparaginyl-tRNA synthetase: coregulation by transcription antitermination?. J Bacteriol. 1996;178:2459-61 pubmed
    ..The nucleotide sequence suggests that asnA and asnS are organized as one operon and regulated by the tRNA-directed transcription antitermination mechanism (T. M. Henkin, Mol. Microbiol. 13:381-387, 1994)...
  39. Gilbert C, Atlan D, Blanc B, Portailer R, Germond J, Lapierre L, et al. A new cell surface proteinase: sequencing and analysis of the prtB gene from Lactobacillus delbruekii subsp. bulgaricus. J Bacteriol. 1996;178:3059-65 pubmed
    ..Finally, the product of the truncated prtM-like gene located immediately upstream of the prtB gene seems too short to be involved in the maturation of PrtB...
  40. Lapierre L, Mollet B, Germond J. Regulation and adaptive evolution of lactose operon expression in Lactobacillus delbrueckii. J Bacteriol. 2002;184:928-35 pubmed
    ..Inactivation of the lac repressor by mutations was then necessary to induce the constitutive expression of the lac genes in L. delbrueckii subsp. bulgaricus...
  41. Morel F, Frot Coutaz J, Aubel D, Portalier R, Atlan D. Characterization of a prolidase from Lactobacillus delbrueckii subsp. bulgaricus CNRZ 397 with an unusual regulation of biosynthesis. Microbiology. 1999;145 ( Pt 2):437-46 pubmed publisher
    ..bulgaricus, PepQ biosynthesis was shown to be dependent on the composition of the culture medium, but not on the peptide concentration. A possible regulation mechanism is discussed...
  42. Le Bras G, Garel J. Pyruvate kinase from Lactobacillus bulgaricus: possible regulation by competition between strong and weak effectors. Biochimie. 1993;75:797-802 pubmed