Lactococcus lactis subsp. lactis Il1403

Summary

Alias: Lactococcus lactis subsp. lactis str. Il1403, Lactococcus lactis subsp. lactis strain Il1403

Top Publications

  1. Buist G, Karsens H, Nauta A, van Sinderen D, Venema G, Kok J. Autolysis of Lactococcus lactis caused by induced overproduction of its major autolysin, AcmA. Appl Environ Microbiol. 1997;63:2722-8 pubmed
    ..After mitomycin induction of an exponential-phase culture of L. lactis LL302 carrying this plasmid, the cells became subject to autolysis, resulting in the release of intracellular proteins. ..
  2. Sissler M, Delorme C, Bond J, Ehrlich S, Renault P, Francklyn C. An aminoacyl-tRNA synthetase paralog with a catalytic role in histidine biosynthesis. Proc Natl Acad Sci U S A. 1999;96:8985-90 pubmed
    ..This observation of a common protein domain linking amino acid biosynthesis and protein synthesis implies an early connection between the biosynthesis of amino acids and proteins. ..
  3. Monedero V, Kuipers O, Jamet E, Deutscher J. Regulatory functions of serine-46-phosphorylated HPr in Lactococcus lactis. J Bacteriol. 2001;183:3391-8 pubmed
    ..It therefore seems that P-Ser-HPr is not essential for inducer expulsion but that in certain cases it can play an indirect role in this regulatory process. ..
  4. Huard C, Miranda G, Redko Y, Wessner F, Foster S, Chapot Chartier M. Analysis of the peptidoglycan hydrolase complement of Lactococcus lactis: identification of a third N-acetylglucosaminidase, AcmC. Appl Environ Microbiol. 2004;70:3493-9 pubmed
    ..Like AcmA and AcmB, AcmC has N-acetylglucosaminidase activity rather than the N-acetylmuramidase activity predicted by sequence similarity. ..
  5. Aleksandrzak Piekarczyk T, Kok J, Renault P, Bardowski J. Alternative lactose catabolic pathway in Lactococcus lactis IL1403. Appl Environ Microbiol. 2005;71:6060-9 pubmed
    ..Moreover, this analysis revealed that although BglS is homologous to a putative phospho-beta-glucosidase, it also exhibits phospho-beta-galactosidase activity and is the major enzyme in L. lactis IL1403 involved in lactose hydrolysis. ..
  6. Aleksandrzak Piekarczyk T, Polak J, Jezierska B, Renault P, Bardowski J. Genetic characterization of the CcpA-dependent, cellobiose-specific PTS system comprising CelB, PtcB and PtcA that transports lactose in Lactococcus lactis IL1403. Int J Food Microbiol. 2011;145:186-94 pubmed publisher
  7. Buist G, Kok J, Leenhouts K, Dabrowska M, Venema G, Haandrikman A. Molecular cloning and nucleotide sequence of the gene encoding the major peptidoglycan hydrolase of Lactococcus lactis, a muramidase needed for cell separation. J Bacteriol. 1995;177:1554-63 pubmed
    ..By replacement recombination, an acmA deletion mutant which grew as long chains was constructed, indicating that AcmA is required for cell separation. ..
  8. Bardowski J, Ehrlich S, Chopin A. BglR protein, which belongs to the BglG family of transcriptional antiterminators, is involved in beta-glucoside utilization in Lactococcus lactis. J Bacteriol. 1994;176:5681-5 pubmed
    ..coli BglG regulator of beta-glucoside utilization. ..
  9. Leenhouts K, Buist G, Bolhuis A, ten Berge A, Kiel J, Mierau I, et al. A general system for generating unlabelled gene replacements in bacterial chromosomes. Mol Gen Genet. 1996;253:217-24 pubmed
    ..A feasibility study was performed using Lactococcus lactis and Bacillus subtilis as model organisms. The results indicate that the method should be applicable to any non-essential gene in numerous bacterial species. ..

More Information

Publications133 found, 100 shown here

  1. Luesink E, van Herpen R, Grossiord B, Kuipers O, de Vos W. Transcriptional activation of the glycolytic las operon and catabolite repression of the gal operon in Lactococcus lactis are mediated by the catabolite control protein CcpA. Mol Microbiol. 1998;30:789-98 pubmed
  2. O Connell Motherway M, van Sinderen D, Morel Deville F, Fitzgerald G, Ehrlich S, Morel P. Six putative two-component regulatory systems isolated from Lactococcus lactis subsp. cremoris MG1363. Microbiology. 2000;146 ( Pt 4):935-47 pubmed
    ..Mutational analysis of the remaining four systems revealed that they are implicated in susceptibility to extreme pH, osmotic or oxidative conditions, or the regulation of phosphatase activity in L. lactis. ..
  3. Steen A, Buist G, Kramer N, Jalving R, Benus G, Venema G, et al. Reduced lysis upon growth of Lactococcus lactis on galactose is a consequence of decreased binding of the autolysin AcmA. Appl Environ Microbiol. 2008;74:4671-9 pubmed publisher
    ..In conclusion, growth of L. lactis on galactose changes the LTA composition in the cell wall in such a way that less AcmA is able to bind to the peptidoglycan, resulting in a decrease in autolysis. ..
  4. Wolfe K. Similarity between putative ATP-binding sites in land plant plastid ORF2280 proteins and the FtsH/CDC48 family of ATPases. Curr Genet. 1994;25:379-83 pubmed
    ..coli FtsH. Phylogenetic analysis suggests that the red and green plastid genes are not true homologues (orthologues) but distinct members of an ancient gene family...
  5. Geller B, Ivey R, Trempy J, Hettinger Smith B. Cloning of a chromosomal gene required for phage infection of Lactococcus lactis subsp. lactis C2. J Bacteriol. 1993;175:5510-9 pubmed
    ..The amino terminus has characteristics of a signal sequence. The putative protein would have a 650-residue, central polar domain. ..
  6. Thoden J, Kim J, Raushel F, Holden H. Structural and kinetic studies of sugar binding to galactose mutarotase from Lactococcus lactis. J Biol Chem. 2002;277:45458-65 pubmed
    ..These different binding modes correlate with both the observed kinetic parameters and the presence or absence of a hydrogen bond between the guanidinium group of Arg-71 and the C-4 hydroxyl group of the sugar ligand. ..
  7. Tremblay L, Zhang G, Dai J, Dunaway Mariano D, Allen K. Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase. J Am Chem Soc. 2005;127:5298-9 pubmed
  8. Magnani D, Barr O, Gerber S, Solioz M. Characterization of the CopR regulon of Lactococcus lactis IL1403. J Bacteriol. 2008;190:536-45 pubmed publisher
    ..When expressed in Escherichia coli, the copRZA operon conferred copper resistance, suggesting that it functions in copper export from the cytoplasm. Other member genes of the CopR regulon may similarly be involved in copper metabolism...
  9. Nilsson D, Kilstrup M. Cloning and expression of the Lactococcus lactis purDEK genes, required for growth in milk. Appl Environ Microbiol. 1998;64:4321-7 pubmed
    ..No secondary transcription start points were mapped in or close to this region, indicating that a putative activator site and not a promoter was deleted or partly destroyed. ..
  10. Frees D, Ingmer H. ClpP participates in the degradation of misfolded protein in Lactococcus lactis. Mol Microbiol. 1999;31:79-87 pubmed
    ..Thus, our data suggest that ClpP plays a major role in the degradation of misfolded proteins...
  11. Bouchard J, Moineau S. Homologous recombination between a lactococcal bacteriophage and the chromosome of its host strain. Virology. 2000;270:65-75 pubmed publisher
    ..We propose that this type of DNA exchange with prophages or remnants of prophages occurs frequently within the P335 species as supported by DNA-DNA comparisons between P335-like phages...
  12. Wang H, Baldwin K, O Sullivan D, McKay L. Identification of a gene cluster encoding Krebs cycle oxidative enzymes linked to the pyruvate carboxylase gene in Lactococcus lactis ssp. lactis C2. J Dairy Sci. 2000;83:1912-8 pubmed
    ..lactis C2. Isocitrate dehydrogenase activity was found to be missing in Lactococcus lactis C2, suggesting that the gene may be incomplete or is not expressed, resulting in a requirement for glutamic acid in lactococci. ..
  13. Gbaguidi B, Mazurkiewicz P, Konings W, Driessen A, Ruysschaert J, Vigano C. Proton motive force mediates a reorientation of the cytosolic domains of the multidrug transporter LmrP. Cell Mol Life Sci. 2004;61:2646-57 pubmed publisher
    ..This drug binding-mediated reorganization may be related to the transition between the high- and low-affinity drug-binding sites and is crucial for drug release in the extracellular medium...
  14. Bauer R, Volschenk H, Dicks L. Cloning and expression of the malolactic gene of Pediococcus damnosus NCFB1832 in Saccharomyces cerevisiae. J Biotechnol. 2005;118:353-62 pubmed
    ..The genetically modified strain of S. cerevisiae decreased the level of L-malate in grape must to less than 0.3 gl(-1) within 3 days. This is the first expression of a malolactic gene from Pediococcus in S. cerevisiae. ..
  15. Nilsson D, Lauridsen A. Isolation of purine auxotrophic mutants of Lactococcus lactis and characterization of the gene hpt encoding hypoxanthine guanine phosphoribosyltransferase. Mol Gen Genet. 1992;235:359-64 pubmed
    ..lactis resulted in an increase in HGPRT activity. In vitro transcription and translation analysis showed that the fragment coded for a polypeptide with M(r) of 22,000. The nucleotide sequence of this hpt gene was determined. ..
  16. Strøman P. Sequence of a gene (lap) encoding a 95.3-kDa aminopeptidase from Lactococcus lactis ssp. cremoris Wg2. Gene. 1992;113:107-12 pubmed
  17. Donkersloot J, Thompson J. Cloning, expression, sequence analysis, and site-directed mutagenesis of the Tn5306-encoded N5-(carboxyethyl)ornithine synthase from Lactococcus lactis K1. J Biol Chem. 1995;270:12226-34 pubmed
    ..A much longer sequence of approximately 80 residues has significant similarity to alanine dehydrogenase. Substitution of arginine 15 of N5-(1-carboxyethyl)-L-ornithine synthase by lysine resulted in loss of enzyme activity. ..
  18. Nardi M, Renault P, Monnet V. Duplication of the pepF gene and shuffling of DNA fragments on the lactose plasmid of Lactococcus lactis. J Bacteriol. 1997;179:4164-71 pubmed
    ..This suggests that the duplication of pepF occurred by recombination from the chromosome of an L. lactis subsp. lactis strain followed by gene transfer. We discuss the possible functions of PepF and the role of its amplification...
  19. Rowland P, Bj rnberg O, Nielsen F, Jensen K, Larsen S. The crystal structure of Lactococcus lactis dihydroorotate dehydrogenase A complexed with the enzyme reaction product throws light on its enzymatic function. Protein Sci. 1998;7:1269-79 pubmed publisher
  20. Tetaud E, Hanau S, Wells J, Le Page R, Adams M, Arkison S, et al. 6-Phosphogluconate dehydrogenase from Lactococcus lactis: a role for arginine residues in binding substrate and coenzyme. Biochem J. 1999;338 ( Pt 1):55-60 pubmed
    ..A second 6-PGDH gene has been identified from the genome of B. subtilis. This second isoform contains an arginine (Arg-34) in this position, suggesting that B. subtilis has two 6-PGDHs with different coenzyme specificities...
  21. Lahiri S, Zhang G, Radstrom P, Dunaway Mariano D, Allen K. Crystallization and preliminary X-ray diffraction studies of beta-phosphoglucomutase from Lactococcus lactus. Acta Crystallogr D Biol Crystallogr. 2002;58:324-6 pubmed
    ..A three-wavelength data set has been collected to 2.3 A on crystals of the SeMet-substituted beta-PGM. The structure solution is currently being attempted by the multiwavelength anomalous diffraction (MAD) phasing method. ..
  22. Coste F, Ober M, Carell T, Boiteux S, Zelwer C, Castaing B. Structural basis for the recognition of the FapydG lesion (2,6-diamino-4-hydroxy-5-formamidopyrimidine) by formamidopyrimidine-DNA glycosylase. J Biol Chem. 2004;279:44074-83 pubmed publisher
    ..The significant differences between the Fpg recognition modes of 8-oxodG and FapydG provide new insights into the Fpg substrate specificity...
  23. Mori S, Mori K, Suzuki I, Kasumi T. Phylogenetic analysis of Lactococcus lactis subspecies based on decoding the sequence of the pepT tripeptidase gene, the pepV dipeptidase gene and 16S rRNA. Syst Appl Microbiol. 2004;27:414-22 pubmed
    ..Considering these results, phylogenetic analysis based on pepT and pepV genes may aid in a more precise index of classification of L. lactis subspecies. PepT and PepV seem to have evolved in similar directions in lactococci. ..
  24. Madsen S, Hindr T, Le Pennec J, Israelsen H, Dufour A. Two acid-inducible promoters from Lactococcus lactis require the cis-acting ACiD-box and the transcription regulator RcfB. Mol Microbiol. 2005;56:735-46 pubmed publisher
    ..The groESL promoter includes a sequence resembling an ACiD-box and the chaperone GroEL production is partly RcfB dependent in acid condition. Our results suggest that the ACiD-box could be the DNA target site of RcfB...
  25. Yinghua Z, Yuting L, Guicheng H. [Expression of cold-shock-protein genes from Lactococcus lactis and analysis of the cryoprotection function]. Wei Sheng Wu Xue Bao. 2008;48:1203-7 pubmed
    ..The 7 kDa cold-shock protein CspD and 6.2 kDa cold-shock protein CspC were identified respectively. The results indicate that CspC improves the recovery of cells and CspD increases the viability after freezing (30-40 folds). ..
  26. Andersen P, Jansen P, Hammer K. Two different dihydroorotate dehydrogenases in Lactococcus lactis. J Bacteriol. 1994;176:3975-82 pubmed
    ..We constructed mutants containing a mutated form of either one or the other or both of the pyrD genes. Only the double mutant is pyrimidine auxotrophic. ..
  27. Erlandson K, Park J, Kao H, Basaran P, Brydges S, Batt C. Dissolution of xylose metabolism in Lactococcus lactis. Appl Environ Microbiol. 2000;66:3974-80 pubmed
    ..Nevertheless, either cumulatively or because of indirect affects on the structures of catalytic sites, these mutations render some strains of L. lactis unable to metabolize xylose...
  28. Boels I, Ramos A, Kleerebezem M, de Vos W. Functional analysis of the Lactococcus lactis galU and galE genes and their impact on sugar nucleotide and exopolysaccharide biosynthesis. Appl Environ Microbiol. 2001;67:3033-40 pubmed publisher
    ..Addition of galactose restored wild-type growth in the galE disruption mutant, while the level of EPS production was approximately one-half the wild-type level...
  29. Kristiansen P, Persson C, Fuochi V, Pedersen A, Karlsson G, Nissen Meyer J, et al. Nuclear Magnetic Resonance Structure and Mutational Analysis of the Lactococcin A Immunity Protein. Biochemistry. 2016;55:6250-6257 pubmed
    ..Truncated variants of LciA in which 6 or 10 of the C-terminal residues were removed yielded a reduced degree of protection, indicating that the unstructured C-terminal tail is important for the functionality of the immunity proteins. ..
  30. van Asseldonk M, Simons A, Visser H, de Vos W, Simons G. Cloning, nucleotide sequence, and regulatory analysis of the Lactococcus lactis dnaJ gene. J Bacteriol. 1993;175:1637-44 pubmed
    ..The conservation of this palindromic sequence in gram-positive heat shock genes suggests a common regulatory pathway distinct from the system used in gram-negative bacteria...
  31. Björnberg O, Viennet T, Skjoldager N, Ćurović A, Nielsen K, Svensson B, et al. Lactococcus lactis thioredoxin reductase is sensitive to light inactivation. Biochemistry. 2015;54:1628-37 pubmed publisher
    ..The significance of this not previously reported oxidation and the exceptionally high rate of oxygen reduction are discussed in relation to other flavin modifications and the possible occurrence of enzymes with similar properties. ..
  32. l Anson K, Movahedi S, Griffin H, Gasson M, Mulholland F. A non-essential glutamyl aminopeptidase is required for optimal growth of Lactococcus lactis MG1363 in milk. Microbiology. 1995;141 ( Pt 11):2873-81 pubmed
    ..Mutants of L. lactis in which the pepA gene was inactivated grew to normal cell densities in milk but exhibited a reduced growth rate during the exponential phase. Thus whilst PepA is required for optimal growth it is not essential. ..
  33. Hellendoorn M, Franke Fayard B, Mierau I, Venema G, Kok J. Cloning and analysis of the pepV dipeptidase gene of Lactococcus lactis MG1363. J Bacteriol. 1997;179:3410-5 pubmed
    ..lactis was constructed by single-crossover recombination. Growth of the mutant strain in milk was significantly slower than that of the wild type, but the strains ultimately reached the same final cell densities. ..
  34. Erlandson K, Batt C. Strain-specific differentiation of lactococci in mixed starter culture populations using randomly amplified polymorphic DNA-derived probes. Appl Environ Microbiol. 1997;63:2702-7 pubmed
    ..The probes for strains 210 and 331 also successfully detected their targets in blended cultures even with a high background of the other two strains. ..
  35. Pr vots F, Tolou S, Delpech B, Kaghad M, Daloyau M. Nucleotide sequence and analysis of the new chromosomal abortive infection gene abiN of Lactococcus lactis subsp. cremoris S114. FEMS Microbiol Lett. 1998;159:331-6 pubmed
    ..63. No homology with any previously described genes was found. A probe was used to determine the presence of this gene only in S114 from 31 strains tested...
  36. Peltonen T, Mantsala P. Isolation and characterization of a purC(orf)QLF operon from Lactococcus [correction of Lactobacillus] lactis MG1614. Mol Gen Genet. 1999;261:31-41 pubmed
    ..4.2.14), can functionally complement the E. coli purF mutant strain TX158. We also show that the promoter of the purC(orf)QLF operon is regulated in response to exogenously added purines. ..
  37. Grossiord B, Luesink E, Vaughan E, Arnaud A, de Vos W. Characterization, expression, and mutation of the Lactococcus lactis galPMKTE genes, involved in galactose utilization via the Leloir pathway. J Bacteriol. 2003;185:870-8 pubmed
    ..Recovery of the wild-type phenotype for the galE mutant was obtained either by genetic complementation or by addition of galactose to the growth medium. ..
  38. N rager S, Arent S, Bj rnberg O, Ottosen M, Lo Leggio L, Jensen K, et al. Lactococcus lactis dihydroorotate dehydrogenase A mutants reveal important facets of the enzymatic function. J Biol Chem. 2003;278:28812-22 pubmed publisher
    ..Furthermore, in one mutant structure we observed differences between the two monomers of the dimer, confirming an apparent asymmetry between the two substrate binding sites that was indicated by the kinetic results...
  39. Dressaire C, Redon E, Milhem H, Besse P, Loubi re P, Cocaign Bousquet M. Growth rate regulated genes and their wide involvement in the Lactococcus lactis stress responses. BMC Genomics. 2008;9:343 pubmed publisher
  40. Petrareanu G, Balasu M, Zander U, Scheidig A, Szedlacsek S. Preliminary X-ray crystallographic analysis of the D-xylulose 5-phosphate phosphoketolase from Lactococcus lactis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010;66:805-7 pubmed publisher
    ..The crystals belonged to the monoclinic space group P2(1). Diffraction data were obtained to a resolution of 2.2 A. ..
  41. Llanos R, Hillier A, Davidson B. Cloning, nucleotide sequence, expression, and chromosomal location of ldh, the gene encoding L-(+)-lactate dehydrogenase, from Lactococcus lactis. J Bacteriol. 1992;174:6956-64 pubmed
    ..lactis subsp. lactis chromosome between coordinates 1.619 and 1.669 of the previously reported physical map (D. L. Tulloch, L. R. Finch, A. J. Hillier, and B. E. Davidson, J. Bacteriol. 173:2768-2775, 1991). ..
  42. Duwat P, Ehrlich S, Gruss A. Use of degenerate primers for polymerase chain reaction cloning and sequencing of the Lactococcus lactis subsp. lactis recA gene. Appl Environ Microbiol. 1992;58:2674-8 pubmed
    ..The end of an open reading frame present upstream of the recA gene shows strong homology with formamidopyrimidine-DNA-glycosylase, a protein involved in DNA repair. ..
  43. Nilsson D, Lauridsen A, Tomoyasu T, Ogura T. A Lactococcus lactis gene encodes a membrane protein with putative ATPase activity that is homologous to the essential Escherichia coli ftsH gene product. Microbiology. 1994;140 ( Pt 10):2601-10 pubmed publisher
    ..Southern hybridization analysis indicated that genes homologous to ftsH of L. lactis were also present in Bacillus subtilis, and several Lactobacillus and Leuconostoc species, suggesting high conservation of ftsH in bacterial species...
  44. Rowland P, Nielsen F, Jensen K, Larsen S. The crystal structure of the flavin containing enzyme dihydroorotate dehydrogenase A from Lactococcus lactis. Structure. 1997;5:239-52 pubmed
    ..The location of the conserved residues surrounding this cavity suggests the potential orientation of the substrate. ..
  45. Rijnen L, Bonneau S, Yvon M. Genetic characterization of the major lactococcal aromatic aminotransferase and its involvement in conversion of amino acids to aroma compounds. Appl Environ Microbiol. 1999;65:4873-80 pubmed
    ..However, another biosynthesis aromatic aminotransferase is induced in the absence of phenylalanine in the culture medium...
  46. Wang H, O Sullivan D, Baldwin K, McKay L. Cloning, sequencing, and expression of the pyruvate carboxylase gene in Lactococcus lactis subsp. lactis C2. Appl Environ Microbiol. 2000;66:1223-7 pubmed
    ..lactis KB4. The deduced lactococcal Pyc protein was highly homologous to Pyc sequences of other bacteria. The pyc gene was also detected in Lactococcus lactis subsp. cremoris and L. lactis subsp. lactis bv. diacetylactis strains. ..
  47. Sanz Y, Lanfermeijer F, Konings W, Poolman B. Kinetics and structural requirements for the binding protein of the Di-tripeptide transport system of Lactococcus lactis. Biochemistry. 2000;39:4855-62 pubmed
    ..This suggests that the ionization of protein residues (pK > 6.0) in or in close proximity to the binding site is critical in the binding mechanism. ..
  48. Bongers R, Hoefnagel M, Starrenburg M, Siemerink M, Arends J, Hugenholtz J, et al. IS981-mediated adaptive evolution recovers lactate production by ldhB transcription activation in a lactate dehydrogenase-deficient strain of Lactococcus lactis. J Bacteriol. 2003;185:4499-507 pubmed
  49. Pritchard G, Freebairn A, Coolbear T. Purification and characterization of an endopeptidase from Lactococcus lactis subsp. cremoris SK11. Microbiology. 1994;140 ( Pt 4):923-30 pubmed
    ..These observations argue against a role for this enzyme in the early stages of casein breakdown by the lactococcal proteolytic system. ..
  50. Tynkkynen S, Buist G, Kunji E, Kok J, Poolman B, Venema G, et al. Genetic and biochemical characterization of the oligopeptide transport system of Lactococcus lactis. J Bacteriol. 1993;175:7523-32 pubmed
    ..The OppA-deficient mutant was unable to transport Leu-enkephalin. Growth experiments with pasteurized milk revealed that transport of oligopeptides forms an essential part of the proteolytic system in lactococci...
  51. Andersen P, Martinussen J, Hammer K. Sequence analysis and identification of the pyrKDbF operon from Lactococcus lactis including a novel gene, pyrK, involved in pyrimidine biosynthesis. J Bacteriol. 1996;178:5005-12 pubmed
    ..Three of these were excluded as members of the pyr operon by insertional analysis (orfA) or by their opposite direction of transcription (orfE and gidB). orfC, however, seems to be the distal gene in the pyrKDbF-orfC operon. ..
  52. Martinussen J, Hammer K. The carB gene encoding the large subunit of carbamoylphosphate synthetase from Lactococcus lactis is transcribed monocistronically. J Bacteriol. 1998;180:4380-6 pubmed
    ..Upstream of the carB gene, an open reading frame showing a high degree of similarity to those of glutathione peroxidases from other organisms was identified. ..
  53. Aungpraphapornchai P, Griffin H, Gasson M. Cloning, DNA sequence analysis, and deletion of a gene encoding diacetyl-acetoin reductase from Lactococcus lactis. DNA Seq. 1999;10:163-72 pubmed
    ..The dar gene has been deleted from the chromosome by double cross-over homologous recombination. ..
  54. Thoden J, Holden H. Molecular structure of galactokinase. J Biol Chem. 2003;278:33305-11 pubmed publisher
    ..Most likely these residues play key roles in catalysis. The structure of galactokinase described here serves as a model for understanding the functional consequences of point mutations known to result in Type II galactosemia in humans...
  55. Shi J, Dertouzos J, Gafni A, Steel D, Palfey B. Single-molecule kinetics reveals signatures of half-sites reactivity in dihydroorotate dehydrogenase A catalysis. Proc Natl Acad Sci U S A. 2006;103:5775-80 pubmed publisher
    ..The present study presents an effective way to explore the subunit catalytic activity and cooperativity of oligomeric enzymes by virtue of single-molecule fluorescence...
  56. Andre G, Leenhouts K, Hols P, Dufr ne Y. Detection and localization of single LysM-peptidoglycan interactions. J Bacteriol. 2008;190:7079-86 pubmed publisher
    ..These results provide novel insight into the binding forces of bacterial LysMs and show that SMFS is a promising tool for studying the heterologous display of proteins or peptides on bacterial surfaces...
  57. Kjos M, Oppegård C, Diep D, Nes I, Veening J, Nissen Meyer J, et al. Sensitivity to the two-peptide bacteriocin lactococcin G is dependent on UppP, an enzyme involved in cell-wall synthesis. Mol Microbiol. 2014;92:1177-87 pubmed publisher
    ..Furthermore, we show that the related class IIb bacteriocin enterocin 1071 also uses UppP as receptor. The approach used here should be broadly applicable to identify receptors for other bacteriocins as well. ..
  58. Aleksandrzak Piekarczyk T, Stasiak Różańska L, Cieśla J, Bardowski J. ClaR--a novel key regulator of cellobiose and lactose metabolism in Lactococcus lactis IL1403. Appl Microbiol Biotechnol. 2015;99:337-47 pubmed publisher
    ..Among 190 of carbon sources tested, the deletion of claR reduces L. lactis growth only in lactose- and/or cellobiose-containing media, suggesting a narrow specificity of this regulator within the context of sugar metabolism. ..
  59. van Asseldonk M, Rutten G, Oteman M, Siezen R, de Vos W, Simons G. Cloning of usp45, a gene encoding a secreted protein from Lactococcus lactis subsp. lactis MG1363. Gene. 1990;95:155-60 pubmed
    ..Usp45 has an unusual aa composition and distribution, and it is predicted to be structurally homologous with P54 of Enterococcus faecium. Up to now, no biological activity could be postulated for this secreted protein...
  60. Cancilla M, Davidson B, Hillier A, Nguyen N, Thompson J. The Lactococcus lactis triosephosphate isomerase gene, tpi, is monocistronic. Microbiology. 1995;141 ( Pt 1):229-38 pubmed
    ..The location of tpi on the L. lactis DL11 chromosome map was determined to be between map coordinates 1.818 and 1.978. ..
  61. O Connell Motherway M, Fitzgerald G, van Sinderen D. Cloning and sequence analysis of putative histidine protein kinases isolated from Lactococcus lactis MG1363. Appl Environ Microbiol. 1997;63:2454-9 pubmed
    ..One of the plasmids also harbored an additional ORF, unrelated to HPKs, with suppressing activity. ..
  62. Ravn P, Arnau J, Madsen S, Vrang A, Israelsen H. The development of TnNuc and its use for the isolation of novel secretion signals in Lactococcus lactis. Gene. 2000;242:347-56 pubmed
    ..Mol. Microbiol. 32 (1999) 75-87]. In all cases, concurrent secretion of nuclease was observed during induction of P170 in a fermentor...
  63. Skinner M, Trempy J. Expression of clpX, an ATPase subunit of the Clp protease, is heat and cold shock inducible in Lactococcus lactis. J Dairy Sci. 2001;84:1783-5 pubmed publisher
    ..8 kDa. Analysis of the relative levels of clpX transcript revealed that in addition to a role in proteolysis of heat damaged proteins, ClpX may also be involved in cryoprotection...
  64. Breüner A, Frees D, Varmanen P, Boguta A, Hammer K, Martinussen J, et al. Ribosomal dimerization factor YfiA is the major protein synthesized after abrupt glucose depletion in Lactococcus lactis. Microbiology. 2016;162:1829-1839 pubmed publisher
  65. Tan P, van Alen Boerrigter I, Poolman B, Siezen R, de Vos W, Konings W. Characterization of the Lactococcus lactis pepN gene encoding an aminopeptidase homologous to mammalian aminopeptidase N. FEBS Lett. 1992;306:9-16 pubmed
    ..A zinc-binding site, as well as the catalytic site for PepN, is predicted to lie within this conserved stretch. Putative promoter regions upstream of PepN were confirmed by primer extension analysis...
  66. Chich J, Chapot Chartier M, Ribadeau Dumas B, Gripon J. Identification of the active site serine of the X-prolyl dipeptidyl aminopeptidase from Lactococcus lactis. FEBS Lett. 1992;314:139-42 pubmed
    ..The consensus sequence surrounding the active site serine in the three known X-prolyl dipeptidyl aminopeptidases (mammalian DPPIV, yeast DPAB and PepX) is G-X-S-Y-X-G, where X is a non-conserved amino acid...
  67. Dodd H, Horn N, Hao Z, Gasson M. A lactococcal expression system for engineered nisins. Appl Environ Microbiol. 1992;58:3683-93 pubmed
    ..Complementation of the lesion in the nisA gene by plasmid-encoded nisA genes containing site-specific mutations resulted in the exclusive production of altered nisins containing specific amino acid substitutions...
  68. El Karoui M, Ehrlich D, Gruss A. Identification of the lactococcal exonuclease/recombinase and its modulation by the putative Chi sequence. Proc Natl Acad Sci U S A. 1998;95:626-31 pubmed
    ..We speculate that exonuclease/recombinase enzymes whose activities are modulated by short DNA sequences are widespread among bacteria...
  69. Andersson U, Levander F, R dstr m P. Trehalose-6-phosphate phosphorylase is part of a novel metabolic pathway for trehalose utilization in Lactococcus lactis. J Biol Chem. 2001;276:42707-13 pubmed publisher
    ..Furthermore, both TrePP and beta-phosphoglucomutase activity were detected in Enterococcus faecalis cell extract, indicating that this bacterium exhibits the same trehalose assimilation route as L. lactis...
  70. Hoefnagel M, Starrenburg M, Martens D, Hugenholtz J, Kleerebezem M, van Swam I, et al. Metabolic engineering of lactic acid bacteria, the combined approach: kinetic modelling, metabolic control and experimental analysis. Microbiology. 2002;148:1003-13 pubmed publisher
    ..Experiments confirmed the predictions of the model, i.e. knocking out lactate dehydrogenase and overexpressing NADH oxidase increased the flux through the acetolactate synthase branch from 0 to 75% of measured product formation rates...
  71. Sperandio B, Polard P, Ehrlich D, Renault P, Gu don E. Sulfur amino acid metabolism and its control in Lactococcus lactis IL1403. J Bacteriol. 2005;187:3762-78 pubmed publisher
  72. Meyrand M, Boughammoura A, Courtin P, M zange C, Guillot A, Chapot Chartier M. Peptidoglycan N-acetylglucosamine deacetylation decreases autolysis in Lactococcus lactis. Microbiology. 2007;153:3275-85 pubmed publisher
    ..In conclusion, enzymic N-acetylglucosamine deacetylation protects peptidoglycan from hydrolysis by the major autolysin AcmA in L. lactis cells, and this leads to decreased cellular autolysis...
  73. Bardowski J, Ehrlich S, Chopin A. Tryptophan biosynthesis genes in Lactococcus lactis subsp. lactis. J Bacteriol. 1992;174:6563-70 pubmed
    ..coli regulates tryptophan biosynthesis in L. lactis, since no potential leader peptide was detected. We propose that a mechanisms resembling that described in Bacillus spp. can regulate trp genes expression in L. lactis...
  74. van Alen Boerrigter I, Baankreis R, de Vos W. Characterization and overexpression of the Lactococcus lactis pepN gene and localization of its product, aminopeptidase N. Appl Environ Microbiol. 1991;57:2555-61 pubmed
    ..The intracellular location of aminopeptidase N in L. lactis was confirmed by immunogold labeling of lactococcal cells...
  75. Monnet V, Nardi M, Chopin A, Chopin M, Gripon J. Biochemical and genetic characterization of PepF, an oligopeptidase from Lactococcus lactis. J Biol Chem. 1994;269:32070-6 pubmed
    ..Preliminary results suggest the presence of a second copy of pepF...
  76. Eaton T, Shearman C, Gasson M. Cloning and sequence analysis of the dnaK gene region of Lactococcus lactis subsp. lactis. J Gen Microbiol. 1993;139:3253-64 pubmed publisher
    ..These repeats are thought to be involved in regulation of the heat-shock genes. The DnaK homologue is induced approximately 3-fold on heat shock at 42 degrees C...
  77. Madsen S, Albrechtsen B, Hansen E, Israelsen H. Cloning and transcriptional analysis of two threonine biosynthetic genes from Lactococcus lactis MG1614. J Bacteriol. 1996;178:3689-94 pubmed
    ..lactis promoter probe vector. In addition, transcriptional studies showed no threonine-dependent regulation of hom-thrB transcription...
  78. Duwat P, Cochu A, Ehrlich S, Gruss A. Characterization of Lactococcus lactis UV-sensitive mutants obtained by ISS1 transposition. J Bacteriol. 1997;179:4473-9 pubmed
  79. Sanders J, Leenhouts K, Burghoorn J, Brands J, Venema G, Kok J. A chloride-inducible acid resistance mechanism in Lactococcus lactis and its regulation. Mol Microbiol. 1998;27:299-310 pubmed
    ..gadR expression is chloride and glutamate independent. A gadR mutant did not produce the 3kb gadCB mRNA that is found in wild-type cells in the presence of NaCl, indicating that GadR is an activator of the gadCB operon...
  80. Luesink E, Beumer C, Kuipers O, de Vos W. Molecular characterization of the Lactococcus lactis ptsHI operon and analysis of the regulatory role of HPr. J Bacteriol. 1999;181:764-71 pubmed
    ..Furthermore, we show for the first time that HPr(Ser-P) functions as a coactivator in the CcpA-mediated catabolite activation of the pyruvate kinase and L-lactate dehydrogenase genes...
  81. Ingmer H, Vogensen F, Hammer K, Kilstrup M. Disruption and analysis of the clpB, clpC, and clpE genes in Lactococcus lactis: ClpE, a new Clp family in gram-positive bacteria. J Bacteriol. 1999;181:2075-83 pubmed
    ..Thus, our data suggest that ClpE, along with ClpP, which recently was shown to participate in the degradation of randomly folded proteins in L. lactis, could be necessary for degrading proteins generated by certain types of stress...
  82. Varmanen P, Ingmer H, Vogensen F. ctsR of Lactococcus lactis encodes a negative regulator of clp gene expression. Microbiology. 2000;146 ( Pt 6):1447-55 pubmed publisher
    ..subtilis, CtsR is a key regulator of heat-shock-induced gene expression, suggesting that the presence of CtsR-homologous DNA-binding sites observed in many Gram-positive bacteria reflects functional heat-shock regulatory systems...
  83. Froger A, Rolland J, Bron P, Lagr e V, Le Cah rec F, Deschamps S, et al. Functional characterization of a microbial aquaglyceroporin. Microbiology. 2001;147:1129-35 pubmed publisher
    ..This result provides important insights to reconstruct the evolutionary history of the MIP family and to elucidate the molecular pathway of water and other solutes in these channels...
  84. Frees D, Varmanen P, Ingmer H. Inactivation of a gene that is highly conserved in Gram-positive bacteria stimulates degradation of non-native proteins and concomitantly increases stress tolerance in Lactococcus lactis. Mol Microbiol. 2001;41:93-103 pubmed
    ..Based on our results, we propose that TrmA, which is well conserved in several Gram-positive bacteria, affects the degradation of non-native proteins and thereby controls stress tolerance...
  85. Boels I, Beerthuyzen M, Kosters M, Van Kaauwen M, Kleerebezem M, de Vos W. Identification and functional characterization of the Lactococcus lactis rfb operon, required for dTDP-rhamnose Biosynthesis. J Bacteriol. 2004;186:1239-48 pubmed
    ..This is the first indication that enzyme activity at the level of central carbohydrate metabolism affects EPS composition...
  86. de la Plaza M, Fern ndez de Palencia P, Pel ez C, Requena T. Biochemical and molecular characterization of alpha-ketoisovalerate decarboxylase, an enzyme involved in the formation of aldehydes from amino acids by Lactococcus lactis. FEMS Microbiol Lett. 2004;238:367-74 pubmed publisher
    ..On the other side, decarboxylation of indole-3-pyruvate and pyruvate only could be detected by a 100-fold increase in the enzyme concentration present in the reaction...
  87. Bolotin A, Quinquis B, Sorokin A, Ehrlich D. Recent genetic transfer between Lactococcus lactis and enterobacteria. J Bacteriol. 2004;186:6671-7 pubmed publisher
    ..Analysis of ycdB gene transfer between two L. lactis subspecies, L. lactis subsp. lactis and L. lactis subsp. cremoris, indicates that the gene can be mobilized, possibly by conjugation...
  88. Siezen R, Starrenburg M, Boekhorst J, Renckens B, Molenaar D, van Hylckama Vlieg J. Genome-scale genotype-phenotype matching of two Lactococcus lactis isolates from plants identifies mechanisms of adaptation to the plant niche. Appl Environ Microbiol. 2008;74:424-36 pubmed publisher
    ..Many of these genes were identified for the first time in Lactococcus lactis. In most cases good correspondence was found with the phenotypic characteristics of these two strains...
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    ..These results show the general importance of chitin-binding proteins in chitin turnover, and provide the first example of a family 33 chitin-binding protein that increases chitinase efficiency towards alpha-chitin...
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  91. Martinussen J, Hammer K. Cloning and characterization of upp, a gene encoding uracil phosphoribosyltransferase from Lactococcus lactis. J Bacteriol. 1994;176:6457-63 pubmed
    ..Secondary mutants in thymidine phosphorylase and thymidine kinase were isolated by selection for resistance to high concentrations of 5-fluorouracil...
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