Enterococcus faecalis V583

Summary

Alias: Enterococcus faecalis str. V583, Enterococcus faecalis strain V583

Top Publications

  1. Deutscher J, Pevec B, Beyreuther K, Kiltz H, Hengstenberg W. Streptococcal phosphoenolpyruvate-sugar phosphotransferase system: amino acid sequence and site of ATP-dependent phosphorylation of HPr. Biochemistry. 1986;25:6543-51 pubmed
    ..The following sequence of 12 amino acid residues of the V8 peptide was determined: -Tyr-Lys-Gly-Lys-Ser-Val-Asn-Leu-Lys-(P)Ser-Ile-Met-.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  2. Evers S, Sahm D, Courvalin P. The vanB gene of vancomycin-resistant Enterococcus faecalis V583 is structurally related to genes encoding D-Ala:D-Ala ligases and glycopeptide-resistance proteins VanA and VanC. Gene. 1993;124:143-4 pubmed
    ..faecalis V583. The DNA fragment hybridized to VmR strains of En. faecium and En. faecalis, but not to their susceptible derivatives...
  3. Audette G, Engelmann R, Hengstenberg W, Deutscher J, Hayakawa K, Quail J, et al. The 1.9 A resolution structure of phospho-serine 46 HPr from Enterococcus faecalis. J Mol Biol. 2000;303:545-53 pubmed
  4. Yan X, Zhao C, Budin Verneuil A, Hartke A, Rinc A, Gilmore M, et al. The (p)ppGpp synthetase RelA contributes to stress adaptation and virulence in Enterococcus faecalis V583. Microbiology. 2009;155:3226-37 pubmed publisher
    ..This virulent phenotype should stem from its increased ability to proliferate under oxidative environments...
  5. B hle L, Mathiesen G, Vaaje Kolstad G, Eijsink V. An endo-?-N-acetylglucosaminidase from Enterococcus faecalis V583 responsible for the hydrolysis of high-mannose and hybrid-type N-linked glycans. FEMS Microbiol Lett. 2011;325:123-9 pubmed publisher
    ..The activity of EF2863 is similar to that of the well known deglycosylating enzyme EndoH from Streptomyces plicatus. According to the CAZy nomenclature, the enzyme is designated EfEndo18A...
  6. Jia Z, Vandonselaar M, Quail J, Delbaere L. Active-centre torsion-angle strain revealed in 1.6 A-resolution structure of histidine-containing phosphocarrier protein. Nature. 1993;361:94-7 pubmed
    ..An HPr phosphotransfer cycling mechanism is proposed, based primarily on the structures of HPr and other phosphotransferase system proteins. ..
  7. Evers S, Courvalin P. Regulation of VanB-type vancomycin resistance gene expression by the VanS(B)-VanR (B) two-component regulatory system in Enterococcus faecalis V583. J Bacteriol. 1996;178:1302-9 pubmed
    ..We have determined the sequence of a 7,160-bp DNA fragment associated with VanB-type resistance in Enterococcus faecalis V583 that contains seven open reading frames...
  8. Kravanja M, Engelmann R, Dossonnet V, Blüggel M, Meyer H, Frank R, et al. The hprK gene of Enterococcus faecalis encodes a novel bifunctional enzyme: the HPr kinase/phosphatase. Mol Microbiol. 1999;31:59-66 pubmed
    ..subtilis enzyme to the kinase activity. A change in activity of the B. subtilis HPr kinase was only observed when fructose-1,6-bisphosphate was also present. ..
  9. J nsson M, Saleihan Z, Nes I, Holo H. Construction and characterization of three lactate dehydrogenase-negative Enterococcus faecalis V583 mutants. Appl Environ Microbiol. 2009;75:4901-3 pubmed publisher
    ..Ldh-2 plays only a minor role in lactate production...

More Information

Publications157 found, 100 shown here

  1. Veith N, Solheim M, van Grinsven K, Olivier B, Levering J, Grosseholz R, et al. Using a genome-scale metabolic model of Enterococcus faecalis V583 to assess amino acid uptake and its impact on central metabolism. Appl Environ Microbiol. 2015;81:1622-33 pubmed publisher
    ..In light of this, we reconstructed a genome-scale metabolic model of the pathogen Enterococcus faecalis V583. The manually curated metabolic network comprises 642 metabolites and 706 reactions...
  2. Gilmore M, Rauch M, Ramsey M, Himes P, Varahan S, Manson J, et al. Pheromone killing of multidrug-resistant Enterococcus faecalis V583 by native commensal strains. Proc Natl Acad Sci U S A. 2015;112:7273-8 pubmed publisher
  3. Li F, Palmer K. EfrEF and the Transcription Regulator ChlR Are Required for Chlorhexidine Stress Response in Enterococcus faecalis V583. Antimicrob Agents Chemother. 2018;62: pubmed publisher
    ..Overall, our study gives insight into E. faecalis stress responses to a commonly used antiseptic. ..
  4. Huycke M, Moore D, Joyce W, Wise P, Shepard L, Kotake Y, et al. Extracellular superoxide production by Enterococcus faecalis requires demethylmenaquinone and is attenuated by functional terminal quinol oxidases. Mol Microbiol. 2001;42:729-40 pubmed
    ..faecalis that results in the formation of hydroxyl radical. These results identify and characterize the mechanism by which E. faecalis generates extracellular free radicals...
  5. Jarzembowski T, Dabrowska Szponar M. [Non-specific adherence of Enterococcus faecalis strains isolated from healthy voluntaries and infection of urinary tract]. Med Dosw Mikrobiol. 2007;59:217-22 pubmed
    ..The result shows ability to differentiate this group by non-specific adherence. ..
  6. An F, Clewell D. Tn917 transposase. Sequence correction reveals a single open reading frame corresponding to the tnpA determinant of Tn3-family elements. Plasmid. 1991;25:121-4 pubmed
    ..The latter exhibits homology with the Tn3-family transposase determinants known as tnpA. ..
  7. Miller R, Adamczyk D, Fyfe J, Elion G. Xanthine phosphoribosyltransferase from Streptococcus faecalis. Properties and specificity. Arch Biochem Biophys. 1974;165:349-58 pubmed
  8. Marcinkeviciene J, Tinney L, Wang K, Rogers M, Copeland R. Dihydroorotate dehydrogenase B of Enterococcus faecalis. Characterization and insights into chemical mechanism. Biochemistry. 1999;38:13129-37 pubmed
    ..8-6.3 and reflects either deprotonation of the reduced flavin or binding of orotate; this step is followed by hydride transfer to C6 and general acid-assisted protonation (pK of 9.1 +/- 0.2) at C5 of the product. ..
  9. Singleton M, H kansson K, Timson D, Wigley D. Structure of the adenylation domain of an NAD+-dependent DNA ligase. Structure. 1999;7:35-42 pubmed
  10. Trinh C, Liu Y, Phillips S, Phillips Jones M. Structure of the response regulator VicR DNA-binding domain. Acta Crystallogr D Biol Crystallogr. 2007;63:266-9 pubmed
  11. Miller H, Mande S, Parsonage D, Sarfaty S, Hol W, Claiborne A. An L40C mutation converts the cysteine-sulfenic acid redox center in enterococcal NADH peroxidase to a disulfide. Biochemistry. 1995;34:5180-90 pubmed
    ..Stereochemical constraints prohibit formation of the Cys41-Cys42 disulfide in the latter mutant...
  12. Ono S, Muratani T, Matsumoto T. Mechanisms of resistance to imipenem and ampicillin in Enterococcus faecalis. Antimicrob Agents Chemother. 2005;49:2954-8 pubmed publisher
    ..Our results suggest that development of high-level resistance to penicillins and imipenem depends on point mutations of PBP4 at positions 520 and 605...
  13. Fischer R, Von Strandmann R, Hengstenberg W. Mannitol-specific phosphoenolpyruvate-dependent phosphotransferase system of Enterococcus faecalis: molecular cloning and nucleotide sequences of the enzyme IIIMtl gene and the mannitol-1-phosphate dehydrogenase gene, expression in Escherichia coli, . J Bacteriol. 1991;173:3709-15 pubmed
    ..faecalis is followed by a stem-loop structure, analogous to a typical Rho-independent terminator. We conclude that the mannitol-specific genes are organized in an operon and that the gene order is mtlA orfX mtlF mtlD...
  14. Hirt H, Wirth R, Muscholl A. Comparative analysis of 18 sex pheromone plasmids from Enterococcus faecalis: detection of a new insertion element on pPD1 and implications for the evolution of this plasmid family. Mol Gen Genet. 1996;252:640-7 pubmed
  15. Oh T, Kwon A, Choi E. Induction of ermAMR from a clinical strain of Enterococcus faecalis by 16-membered-ring macrolide antibiotics. J Bacteriol. 1998;180:5788-91 pubmed
  16. Wu R, Zhang R, Zagnitko O, Dementieva I, Maltzev N, Watson J, et al. Crystal structure of Enterococcus faecalis SlyA-like transcriptional factor. J Biol Chem. 2003;278:20240-4 pubmed publisher
    ..Phylogenetic analysis of the MarR/SlyA family suggests that Sly is placed between the SlyA and MarR subfamilies and shows significant sequence similarity to members of both subfamilies...
  17. Michaux C, Martini C, Hanin A, Auffray Y, Hartke A, Giard J. SlyA regulator is involved in bile salts stress response of Enterococcus faecalis. FEMS Microbiol Lett. 2011;324:142-6 pubmed publisher
    ..To increase knowledge of SlyA regulon, real-time quantitative PCR was performed and revealed that expression of EF_3005, which encodes a choloylglycine hydrolase, is negatively regulated by SlyA. ..
  18. Chalansonnet V, Mercier C, Orenga S, Gilbert C. Identification of Enterococcus faecalis enzymes with azoreductases and/or nitroreductase activity. BMC Microbiol. 2017;17:126 pubmed publisher
    ..Four putative nitroreductases, EF0404, EF0648, EF0655 and EF1181 from Enterococcus faecalis V583 were overexpressed as his-tagged recombinant proteins in Escherichia coli and purified following a ..
  19. Ahmed S, Claiborne A. The streptococcal flavoprotein NADH oxidase. I. Evidence linking NADH oxidase and NADH peroxidase cysteinyl redox centers. J Biol Chem. 1989;264:19856-63 pubmed
  20. Charrier V, Buckley E, Parsonage D, Galinier A, Darbon E, Jaquinod M, et al. Cloning and sequencing of two enterococcal glpK genes and regulation of the encoded glycerol kinases by phosphoenolpyruvate-dependent, phosphotransferase system-catalyzed phosphorylation of a single histidyl residue. J Biol Chem. 1997;272:14166-74 pubmed
    ..Fructose 1,6-bisphosphate was found to inhibit E. casseliflavus glycerol kinase activity. However, neither EIIAGlc from E. coli nor the EIIAGlc domain of Bacillus subtilis had an inhibitory effect on glycerol kinase of E. casseliflavus...
  21. Matveyev A, Young K, Meng A, Elhai J. DNA methyltransferases of the cyanobacterium Anabaena PCC 7120. Nucleic Acids Res. 2001;29:1491-506 pubmed
    ..It is structurally unusual and along with a few proteins of prokaryotic and eukaryotic origin defines a structural class of MTases distinct from all previously described...
  22. Rumpel S, Razeto A, Pillar C, Vijayan V, Taylor A, Giller K, et al. Structure and DNA-binding properties of the cytolysin regulator CylR2 from Enterococcus faecalis. EMBO J. 2004;23:3632-42 pubmed publisher
    ..Our results propose a mechanism where repression is achieved by CylR2 obstruction of the promoter preventing biosynthesis of the cytolysin operon transcript...
  23. Coburn P, Pillar C, Jett B, Haas W, Gilmore M. Enterococcus faecalis senses target cells and in response expresses cytolysin. Science. 2004;306:2270-2 pubmed publisher
    ..Thus, enterococci use CylL(L)'' to actively probe the environment for target cells, and when target cells are detected, allows the organism to express high levels of cytolysin in response...
  24. Latorre M, Olivares F, Reyes Jara A, López G, Gonzalez M. CutC is induced late during copper exposure and can modify intracellular copper content in Enterococcus faecalis. Biochem Biophys Res Commun. 2011;406:633-7 pubmed publisher
    ..faecalis. This bacterium is the first reported organism containing a cop operon and an active member of the Cut protein family. ..
  25. Mehmeti I, Faergestad E, Bekker M, Snipen L, Nes I, Holo H. Growth rate-dependent control in Enterococcus faecalis: effects on the transcriptome and proteome, and strong regulation of lactate dehydrogenase. Appl Environ Microbiol. 2012;78:170-6 pubmed publisher
    b>Enterococcus faecalis V583 was grown in a glucose-limited chemostat at three different growth rates (0.05, 0.15, and 0.4 h?¹)...
  26. Li X, Weinstock G, Murray B. Generation of auxotrophic mutants of Enterococcus faecalis. J Bacteriol. 1995;177:6866-73 pubmed
    ..coli, was introduced into the E. faecalis chromosome en masse, and an auxotroph was obtained. These results demonstrate a new methodology for constructing defined knockout mutations in E. faecalis...
  27. Rana N, Sauvageot N, Laplace J, Bao Y, Nes I, Rinc A, et al. Redox balance via lactate dehydrogenase is important for multiple stress resistance and virulence in Enterococcus faecalis. Infect Immun. 2013;81:2662-8 pubmed publisher
    ..J. Libby, and F. C. Fang, Science 319:1672-1676, 2008), identify LDH as an attractive drug target. These drugs may have additional applications, as in the fight against glycopeptide antibiotic-resistant bacteria and even cancer...
  28. Leboeuf C, Auffray Y, Hartke A. Cloning, sequencing and characterization of the ccpA gene from Enterococcus faecalis. Int J Food Microbiol. 2000;55:109-13 pubmed
    ..Here, we report the cloning and characterisation of the chromosomal ccpA gene from Enterococcus faecalis JH2-2. This gene is predicted to encode a 333 amino acids protein with nearly 75% identity to CcpA of Lactobacillus casei. ..
  29. Laplace J, Hartke A, Giard J, Auffray Y. Cloning, characterization and expression of an Enterococcus faecalis gene responsive to heavy metals. Appl Microbiol Biotechnol. 2000;53:685-9 pubmed
    ..Our results suggest that biosensors based on E. faecalis may have potential applications for environmental monitoring and should be constructed...
  30. Shaw J, Clewell D. Complete nucleotide sequence of macrolide-lincosamide-streptogramin B-resistance transposon Tn917 in Streptococcus faecalis. J Bacteriol. 1985;164:782-96 pubmed
    ..Transcription analyses resulted in data consistent with this interpretation...
  31. Marina A, Bravo J, Fita I, Rubio V. Crystallization, characterization and preliminary crystallographic studies of carbamate kinase of Streptococcus faecium. J Mol Biol. 1994;235:1345-7 pubmed
  32. Xu Y, Murray B, Weinstock G. A cluster of genes involved in polysaccharide biosynthesis from Enterococcus faecalis OG1RF. Infect Immun. 1998;66:4313-23 pubmed
    ..Insertions in several genes within the cluster abolished the immunoreactivity of the clone. This is the first report on a gene cluster of E. faecalis involved in the biosynthesis of an antigenic polysaccharide...
  33. Kishor C, Arya T, Reddi R, Chen X, Saddanapu V, Marapaka A, et al. Identification, biochemical and structural evaluation of species-specific inhibitors against type I methionine aminopeptidases. J Med Chem. 2013;56:5295-305 pubmed publisher
    ..Crystal structures of four selected inhibitors in complex with human MetAP1b and molecular modeling studies provided the basis for the binding specificity. ..
  34. Marina A, Alzari P, Bravo J, Uriarte M, Barcelona B, Fita I, et al. Carbamate kinase: New structural machinery for making carbamoyl phosphate, the common precursor of pyrimidines and arginine. Protein Sci. 1999;8:934-40 pubmed publisher
    ..The orientation of the crevices in the dimer precludes their physical cooperation in the catalytic process. Such cooperation is not needed in the CK reaction but is a requirement of the mechanism of CPSs...
  35. Collin M, Fischetti V. A novel secreted endoglycosidase from Enterococcus faecalis with activity on human immunoglobulin G and ribonuclease B. J Biol Chem. 2004;279:22558-70 pubmed publisher
    ..faecalis, EndoE, which by two distinct activities hydrolyzes the glycans on RNase B and IgG. Both activities could be important for the molecular pathogenesis and persistence of E. faecalis during human infections...
  36. Poole L, Claiborne A. The non-flavin redox center of the streptococcal NADH peroxidase. II. Evidence for a stabilized cysteine-sulfenic acid. J Biol Chem. 1989;264:12330-8 pubmed
  37. Lu J, Perng C, Ho M, Chiueh T, Lee W. High prevalence of VanB2 vancomycin-resistant Enterococcus faecium in Taiwan. J Clin Microbiol. 2001;39:2140-5 pubmed publisher
    ..This result suggests that intra- and interhospital dissemination of this E. faecium strain has occurred in Taiwan...
  38. Ferretti J, Gilmore K, Courvalin P. Nucleotide sequence analysis of the gene specifying the bifunctional 6'-aminoglycoside acetyltransferase 2"-aminoglycoside phosphotransferase enzyme in Streptococcus faecalis and identification and cloning of gene regions specifying the two activitie. J Bacteriol. 1986;167:631-8 pubmed
    ..These data suggest that the gene specifying the AAC(6')-APH(2") resistance enzyme arose as a result of a gene fusion...
  39. Crepin T, Yaremchuk A, Tukalo M, Cusack S. Structures of two bacterial prolyl-tRNA synthetases with and without a cis-editing domain. Structure. 2006;14:1511-25 pubmed publisher
    ..The structures give insight into the complex evolution of ProRSs, the mechanism of editing, and structural differences between prokaryotic- and eukaryotic-type ProRSs that can be exploited for antibiotic design...
  40. Bonafede M, Carias L, Rice L. Enterococcal transposon Tn5384: evolution of a composite transposon through cointegration of enterococcal and staphylococcal plasmids. Antimicrob Agents Chemother. 1997;41:1854-8 pubmed
    ..These results implicate broad-host-range plasmids in the transfer of resistance determinants from staphylococci to enterococci. ..
  41. Hosaka T, Meguro T, Yamato I, Shirakihara Y. Crystal structure of Enterococcus hirae enolase at 2.8 A resolution. J Biochem. 2003;133:817-23 pubmed
    ..Our findings show that the three dimensional structure of an important enzyme in the glycolytic pathway is evolutionarily conserved among eukaryotes and prokaryotes, including gram-positive bacteria. ..
  42. Zou J, Baghdayan A, Payne S, Shankar N. A TIR domain protein from E. faecalis attenuates MyD88-mediated signaling and NF-κB activation. PLoS ONE. 2014;9:e112010 pubmed publisher
    ..faecalis) was identified in the genome of Enterococcus faecalis V583, and predicted to encode a protein resembling mammalian and bacterial TIR proteins...
  43. An F, Clewell D. Characterization of the determinant (traB) encoding sex pheromone shutdown by the hemolysin/bacteriocin plasmid pAD1 in Enterococcus faecalis. Plasmid. 1994;31:215-21 pubmed publisher
    ..The deduced product has a mass of 43.7 kDa with the C-terminal third consisting primarily of hydrophobic amino acid residues...
  44. Low Y, Jakubovics N, Flatman J, Jenkinson H, Smith A. Manganese-dependent regulation of the endocarditis-associated virulence factor EfaA of Enterococcus faecalis. J Med Microbiol. 2003;52:113-9 pubmed publisher
    ..The results identify a new Mn(2+)-sensing regulator in enterococci that regulates the expression of a virulence factor implicated in enterococcal endocarditis...
  45. Lorenzo Díaz F, Delgado T, Reyes Darias J, Flores C, Mendez Alvarez S, Villar J, et al. Characterization of the first VanB vancomycin-resistant Enterococcus faecium isolated in a Spanish hospital. Curr Microbiol. 2004;48:199-203 pubmed
    ..Sequence analysis of the vanB gene showed that this isolate belonged to subtype vanB2. Moreover, PCR amplification analysis indicated that the vanB gene cluster was linked to a Tn5382-like transposon. ..
  46. Reinelt S, Koch B, Hothorn M, Hengstenberg W, Welti S, Scheffzek K. Structure of the Enterococcus faecalis EIIA(gnt) PTS component. Biochem Biophys Res Commun. 2009;388:626-9 pubmed publisher
    ..We therefore propose that the phosphoryl transfer reactions involving EIIA(gnt) proceed according to a mechanism analog to the one described for E. coli EIIA(man). ..
  47. Maurer T, D ker R, G rler A, Hengstenberg W, Kalbitzer H. Three-dimensional structure of the histidine-containing phosphocarrier protein (HPr) from Enterococcus faecalis in solution. Eur J Biochem. 2001;268:635-44 pubmed
    ..The observed torsional strain must either be a crystallization artefact or represents a conformational state that exists only to a small extent in solution...
  48. Onodera Y, Okuda J, Tanaka M, Sato K. Inhibitory activities of quinolones against DNA gyrase and topoisomerase IV of Enterococcus faecalis. Antimicrob Agents Chemother. 2002;46:1800-4 pubmed
    ..These results indicated that other factors can modulate the effect of target affinity to determine the bacterial killing pathway, but the highest inhibitory actions against both enzymes correlated with good antienterococcal activities. ..
  49. Weaver K, Tritle D. Identification and characterization of an Enterococcus faecalis plasmid pAD1-encoded stability determinant which produces two small RNA molecules necessary for its function. Plasmid. 1994;32:168-81 pubmed publisher
    ..Mutations affecting par that resulted in a decrease in plasmid stability also resulted in changes in the pattern of production of the par RNAs, suggesting that these RNAs are important for par function...
  50. Ross R, Claiborne A. Cloning, sequence and overexpression of NADH peroxidase from Streptococcus faecalis 10C1. Structural relationship with the flavoprotein disulfide reductases. J Mol Biol. 1991;221:857-71 pubmed
    ..coli genetic background. The resultant overexpressing clone produced a recombinant NPXase that was catalytically active and immunoreactive to NPXase antisera...
  51. Geiss Liebisch S, Rooijakkers S, Beczala A, Sanchez Carballo P, Kruszynska K, Repp C, et al. Secondary cell wall polymers of Enterococcus faecalis are critical for resistance to complement activation via mannose-binding lectin. J Biol Chem. 2012;287:37769-77 pubmed publisher
    ..Our study indicates that in E. faecalis, modification of peptidoglycan by secondary cell wall polymers is critical to evade recognition by the complement system...
  52. Chung J, Bensing B, Dunny G. Genetic analysis of a region of the Enterococcus faecalis plasmid pCF10 involved in positive regulation of conjugative transfer functions. J Bacteriol. 1995;177:2107-17 pubmed
    ..faecalis but that outward-reading transcripts may initiate from within the ends of Tn5 or from the junction sequences...
  53. Ghim S, Kim C, Bonner E, D Elia J, Grabner G, Switzer R. The Enterococcus faecalis pyr operon is regulated by autogenous transcriptional attenuation at a single site in the 5' leader. J Bacteriol. 1999;181:1324-9 pubmed
    ..Several lines of evidence demonstrate that the E. faecalis pyr operon is repressed by uracil via transcriptional attenuation at the single 5' leader termination site and that attenuation is mediated by the PyrR protein...
  54. Crane E, Yeh J, Luba J, Claiborne A. Analysis of the kinetic and redox properties of the NADH peroxidase R303M mutant: correlation with the crystal structure. Biochemistry. 2000;39:10353-64 pubmed
    ..These and other results provide a detailed understanding of Arg303 and its role in the structure and mechanism of this unique flavoprotein peroxidase. ..
  55. Werner G, Hildebrandt B, Klare I, Witte W. Linkage of determinants for streptogramin A, macrolide-lincosamide-streptogramin B, and chloramphenicol resistance on a conjugative plasmid in Enterococcus faecium and dissemination of this cluster among streptogramin-resistant enterococci. Int J Med Microbiol. 2000;290:543-8 pubmed publisher
  56. Teng L, Hsueh P, Wang Y, Lin H, Luh K, Ho S. Determination of Enterococcus faecalis groESL full-length sequence and application for species identification. J Clin Microbiol. 2001;39:3326-31 pubmed
    ..The DNA-based assays developed in this study provide an alternative to currently used methods of identification for clinically important enterococcal species. ..
  57. Duez C, Zorzi W, Sapunaric F, Amoroso A, Thamm I, Coyette J. The penicillin resistance of Enterococcus faecalis JH2-2r results from an overproduction of the low-affinity penicillin-binding protein PBP4 and does not involve a psr-like gene. Microbiology. 2001;147:2561-9 pubmed
    ..faecalis strains indicated that they were identical. It is therefore postulated that the PBP4 overproduction in E. faecalis JH2-2r results from the modification of an as yet unidentified factor. ..
  58. Hasman H, Aarestrup F. tcrB, a gene conferring transferable copper resistance in Enterococcus faecium: occurrence, transferability, and linkage to macrolide and glycopeptide resistance. Antimicrob Agents Chemother. 2002;46:1410-6 pubmed
    ..The frequent occurrence of this new copper resistance gene in isolates from pigs, where copper sulfate is being used in large amounts as feed additive, suggests that the use of copper has selected for resistance...
  59. Shibata C, Ehara T, Tomura K, Igarashi K, Kobayashi H. Gene structure of Enterococcus hirae (Streptococcus faecalis) F1F0-ATPase, which functions as a regulator of cytoplasmic pH. J Bacteriol. 1992;174:6117-24 pubmed
    ..coli. Deduced amino acid sequences suggested that E. hirae H(+)-ATPase is a typical F1F0-type ATPase but that its gene structure is not identical to that of other bacterial F1F0-ATPases. ..
  60. Poole L, Claiborne A. Interactions of pyridine nucleotides with redox forms of the flavin-containing NADH peroxidase from Streptococcus faecalis. J Biol Chem. 1986;261:14525-33 pubmed
    ..Either the EH2 X NAD+ or the EH2 X NAD+ X NADH forms may be involved in the catalytic mechanism of the enzyme, as both are reactive with hydrogen peroxide. ..
  61. Mande S, Parsonage D, Claiborne A, Hol W. Crystallographic analyses of NADH peroxidase Cys42Ala and Cys42Ser mutants: active site structures, mechanistic implications, and an unusual environment of Arg 303. Biochemistry. 1995;34:6985-92 pubmed
    ..8 A, suggesting a favorable electrostatic interaction between Arg 303 and Tyr 60.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  62. Crane E, Vervoort J, Claiborne A. 13C NMR analysis of the cysteine-sulfenic acid redox center of enterococcal NADH peroxidase. Biochemistry. 1997;36:8611-8 pubmed
    ..These results provide direct evidence in support of the peroxidase Cys42-sulfenic acid/thiol redox cycle and add significantly to our structure-based understanding of protein-sulfenic acid stabilization and function. ..
  63. Guiton P, Hung C, Kline K, Roth R, Kau A, Hayes E, et al. Contribution of autolysin and Sortase a during Enterococcus faecalis DNA-dependent biofilm development. Infect Immun. 2009;77:3626-38 pubmed publisher
    ..This study establishes the stage-specific requirements for SrtA and Atn and demonstrates a role for Atn in the pathway leading to DNA release during biofilm development in E. faecalis...
  64. Beliveau C, Potvin C, Trudel J, Asselin A, Bellemare G. Cloning, sequencing, and expression in Escherichia coli of a Streptococcus faecalis autolysin. J Bacteriol. 1991;173:5619-23 pubmed
    ..Sequencing of a portion of pSH4000 revealed a unique open reading frame of 2,013 nucleotides coding for a 641-amino-acid (74-kDa) polypeptide and containing four 204-nucleotide direct repeats. ..
  65. Poole L, Claiborne A. The non-flavin redox center of the streptococcal NADH peroxidase. I. Thiol reactivity and redox behavior in the presence of urea. J Biol Chem. 1989;264:12322-9 pubmed
    ..We propose that low concentrations of urea stabilize an intermediate state in the transition between native and denatured forms, which is responsible for the observed changes in both active-site thiol reactivity and in redox properties. ..
  66. Claiborne A, Miller H, Parsonage D, Ross R. Protein-sulfenic acid stabilization and function in enzyme catalysis and gene regulation. FASEB J. 1993;7:1483-90 pubmed
    ..16-A structure of the streptococcal NADH peroxidase, provide a molecular basis for understanding the proposed redox functions of these novel cofactors in both enzyme catalysis and transcriptional regulation...
  67. Lee M, Nittayajarn A, Ross R, Rothschild C, Parsonage D, Claiborne A, et al. Characterization of Enterococcus faecalis alkaline phosphatase and use in identifying Streptococcus agalactiae secreted proteins. J Bacteriol. 1999;181:5790-9 pubmed
    ..AP acts as a reporter enzyme in GBS, GAS, and E. faecalis and is expected to be useful in a variety of gram-positive bacteria...
  68. Ballo M, Rtimi S, Mancini S, Kiwi J, Pulgarin C, Entenza J, et al. Bactericidal activity and mechanism of action of copper-sputtered flexible surfaces against multidrug-resistant pathogens. Appl Microbiol Biotechnol. 2016;100:5945-53 pubmed publisher
    ..The decrease in cell viability of MRSA ATCC 43300 and Enterococcus faecalis V583 correlated with the progressive loss of cytoplasmic membrane integrity both under aerobic and anaerobic ..
  69. Ross R, Claiborne A. Molecular cloning and analysis of the gene encoding the NADH oxidase from Streptococcus faecalis 10C1. Comparison with NADH peroxidase and the flavoprotein disulfide reductases. J Mol Biol. 1992;227:658-71 pubmed
  70. Rice L, Carias L. Transfer of Tn5385, a composite, multiresistance chromosomal element from Enterococcus faecalis. J Bacteriol. 1998;180:714-21 pubmed
    ..They also suggest that chromosomal recombination is a mechanism of genetic exchange in enterococci...
  71. Lee J, Chang C, Song H, Moon J, Yang J, Kim H, et al. Crystal structure of NAD(+)-dependent DNA ligase: modular architecture and functional implications. EMBO J. 2000;19:1119-29 pubmed publisher
    ..It also provides clues for protein flexibility and DNA-binding sites. A model for the multidomain ligase action involving large conformational changes is proposed...
  72. Nallapareddy S, Singh K, Duh R, Weinstock G, Murray B. Diversity of ace, a gene encoding a microbial surface component recognizing adhesive matrix molecules, from different strains of Enterococcus faecalis and evidence for production of ace during human infections. Infect Immun. 2000;68:5210-7 pubmed
    ..faecalis, with at least four variants related to the differences in the B domain, is expressed by different strains during infection in humans, and human-derived antibodies can block adherence to these extracellular matrix proteins...
  73. Mohamed J, Huang W, Nallapareddy S, Teng F, Murray B. Influence of origin of isolates, especially endocarditis isolates, and various genes on biofilm formation by Enterococcus faecalis. Infect Immun. 2004;72:3658-63 pubmed publisher
    ..faecalis regulator) [corrected] resulted in fewer attached bacteria, as determined using phase-contrast microscopy, and less biofilm (P < 0.0001)...
  74. Steussy C, Robison A, Tetrick A, Knight J, Rodwell V, Stauffacher C, et al. A structural limitation on enzyme activity: the case of HMG-CoA synthase. Biochemistry. 2006;45:14407-14 pubmed publisher
    ..In addition, the hydroxyl of Ser308 rotates 120 degrees to a position where it is able to stabilize the carbanion intermediate formed by the methyl group of the acetyl-S-enzyme during its condensation with acetoacetyl-CoA...
  75. Tomita H, Kamei E, Ike Y. Cloning and genetic analyses of the bacteriocin 41 determinant encoded on the Enterococcus faecalis pheromone-responsive conjugative plasmid pYI14: a novel bacteriocin complemented by two extracellular components (lysin and activator). J Bacteriol. 2008;190:2075-85 pubmed publisher
  76. Stehle T, Ahmed S, Claiborne A, Schulz G. The structure of NADH peroxidase from Streptococcus faecalis at 3.3 A resolution. FEBS Lett. 1990;267:186-8 pubmed
    ..FAD binds in the same region and in a similar conformation as in glutathione reductase. The unusual cysteine-sulfenic acid participating in catalysis is located at the isoalloxazine of FAD. ..
  77. Raze D, Dardenne O, Hallut S, Martinez Bueno M, Coyette J, Ghuysen J. The gene encoding the low-affinity penicillin-binding protein 3r in Enterococcus hirae S185R is borne on a plasmid carrying other antibiotic resistance determinants. Antimicrob Agents Chemother. 1998;42:534-9 pubmed
    ..faecium strain and inserted in a plasmid of E. hirae. E. hirae is the first known bacterial species in which a low-affinity PBP-encoding gene has been found to be plasmid borne. ..
  78. Qin X, Singh K, Weinstock G, Murray B. Effects of Enterococcus faecalis fsr genes on production of gelatinase and a serine protease and virulence. Infect Immun. 2000;68:2579-86 pubmed
    ..These results suggest that sprE and agr-like genes contribute to the virulence of E. faecalis OG1RF in this model...
  79. Dahl K, Lundblad E, Rokenes T, Olsvik O, Sundsfjord A. Genetic linkage of the vanB2 gene cluster to Tn5382 in vancomycin-resistant enterococci and characterization of two novel insertion sequences. Microbiology. 2000;146 ( Pt 6):1469-79 pubmed publisher
    ..These isolates showed related PFGE patterns, suggesting possible clonal spread of a VRE strain harbouring a Tn5382-vanB2-ISEnfa200 element linked to a pbp5 gene conferring ampicillin resistance...
  80. Barcelona Andr s B, Marina A, Rubio V. Gene structure, organization, expression, and potential regulatory mechanisms of arginine catabolism in Enterococcus faecalis. J Bacteriol. 2002;184:6289-300 pubmed
    ..Of the three other genes identified, two form a transcriptional unit and encode a putative metal-sensitive transcriptional regulator (ArsR) and a cysteine protease...
  81. Foster K, Barnes M, Stephenson R, Butler M, Skow D, LaMarr W, et al. DNA polymerase III of Enterococcus faecalis: expression and characterization of recombinant enzymes encoded by the polC and dnaE genes. Protein Expr Purif. 2003;27:90-7 pubmed
    ..In conclusion, the properties of each Enterococcus polymerase enzymes were similar to those of the respective B. subtilis enzymes. ..
  82. Brockmeier A, Skopnik M, Koch B, Herrmann C, Hengstenberg W, Welti S, et al. Activity of the Enterococcus faecalis EIIA(gnt) PTS component and its strong interaction with EIIB(gnt). Biochem Biophys Res Commun. 2009;388:630-6 pubmed publisher
  83. Park Y, Jung M, Song H, Jeong K, Bang E, Hwang G, et al. Novel Structural Components Contribute to the High Thermal Stability of Acyl Carrier Protein from Enterococcus faecalis. J Biol Chem. 2016;291:1692-702 pubmed publisher
    ..These results might provide insights into the development of antibiotics against pathogenic drug-resistant E. faecalis strains. ..
  84. Allen A, Perham R. Two lipoyl domains in the dihydrolipoamide acetyltransferase chain of the pyruvate dehydrogenase multienzyme complex of Streptococcus faecalis. FEBS Lett. 1991;287:206-10 pubmed
    ..The S. faecalis E2 chain differs in the number of its lipoyl domains from the E2 chains of all bacterial pyruvate dehydrogenase complexes hitherto described. ..
  85. Jia Z, Vandonselaar M, Hengstenberg W, Quail J, Delbaere L. The 1.6 A structure of histidine-containing phosphotransfer protein HPr from Streptococcus faecalis. J Mol Biol. 1994;236:1341-55 pubmed
    ..The putative involvement of the C-terminal alpha-carboxyl group and the related Gly67-Glu70 reverse beta-turn with respect to the function of HPr are described...
  86. Weaver K. Enterococcus faecalis plasmid pAD1 replication and maintenance. Dev Biol Stand. 1995;85:89-98 pubmed
    ..The isolated par determinant was capable of producing both RNAs and stabilizing a heterologous vector, indicating that no other pAD1-encoded determinants are required for par function...
  87. Heaton M, Discotto L, Pucci M, Handwerger S. Mobilization of vancomycin resistance by transposon-mediated fusion of a VanA plasmid with an Enterococcus faecium sex pheromone-response plasmid. Gene. 1996;171:9-17 pubmed
    ..Similar IS1216V-mediated transposition events may contribute to the horizontal spread of glycopeptide resistance among enterococci in nature...
  88. Bo l G, Pichereau V, Mijakovic I, Maz A, Poncet S, Gillet S, et al. Is 2-phosphoglycerate-dependent automodification of bacterial enolases implicated in their export?. J Mol Biol. 2004;337:485-96 pubmed publisher
    ..The K341E mutant enolase was almost as active as the wild-type enzyme and therefore allowed us to establish that the loss of enolase export correlates with the loss of modification and not the loss of glycolytic activity...
  89. Ozawa Y, De Boever E, Clewell D. Enterococcus faecalis sex pheromone plasmid pAM373: analyses of TraA and evidence for its interaction with RpoB. Plasmid. 2005;54:57-69 pubmed publisher
    ..Evidence is also presented suggesting that this regulation at t(ac) also involves an additional, possibly cis-acting, element...
  90. Mohamed J, Teng F, Nallapareddy S, Murray B. Pleiotrophic effects of 2 Enterococcus faecalis sagA-like genes, salA and salB, which encode proteins that are antigenic during human infection, on biofilm formation and binding to collagen type i and fibronectin. J Infect Dis. 2006;193:231-40 pubmed publisher
  91. Emirian A, Fromentin S, Eckert C, Chau F, Dubost L, Delepierre M, et al. Impact of peptidoglycan O-acetylation on autolytic activities of the Enterococcus faecalis N-acetylglucosaminidase AtlA and N-acetylmuramidase AtlB. FEBS Lett. 2009;583:3033-8 pubmed publisher
    ..In contrast, removal of cell wall glycopolymers increased the AtlB activity (37-fold), suggesting that these polymers negatively control the activity of this enzyme...
  92. Signoretto C, Boaretti M, Canepari P. Cloning, sequencing and expression in Escherichia coli of the low-affinity penicillin binding protein of Enterococcus faecalis. FEMS Microbiol Lett. 1994;123:99-106 pubmed
    ..faecalis strains, thus suggesting that genes coding for low-affinity PBPs of enterococci are not strictly homologous. In this experiment digoxigenin-labelled E. faecalis DNA was used...