Bacillus licheniformis DSM 13 = ATCC 14580

Summary

Alias: Bacillus licheniformis ATCC 14580, Bacillus licheniformis ATCC 14580 = DSM 13, Bacillus licheniformis DSM 13

Top Publications

  1. Wittman V, Wong H. Regulation of the penicillinase genes of Bacillus licheniformis: interaction of the pen repressor with its operators. J Bacteriol. 1988;170:3206-12 pubmed
  2. Melckebeke H, Vreuls C, Gans P, Filee P, Llabres G, Joris B, et al. Solution structural study of BlaI: implications for the repression of genes involved in beta-lactam antibiotic resistance. J Mol Biol. 2003;333:711-20 pubmed
    ..These results help toward understanding the repression and induction mechanism of the genes coding for beta-lactamase and PBP2'. ..
  3. Kakudo S, Kikuchi N, Kitadokoro K, Fujiwara T, Nakamura E, Okamoto H, et al. Purification, characterization, cloning, and expression of a glutamic acid-specific protease from Bacillus licheniformis ATCC 14580. J Biol Chem. 1992;267:23782-8 pubmed
    A glutamic acid-specific protease has been purified to homogeneity from Bacillus licheniformis ATCC 14580 utilizing Phe-Leu-D-Glu-OMe-Sepharose affinity chromatography and crystallized...
  4. Kandra L, Gyemant G, Remenyik J, Hovánszki G, Lipták A. Action pattern and subsite mapping of Bacillus licheniformis alpha-amylase (BLA) with modified maltooligosaccharide substrates. FEBS Lett. 2002;518:79-82 pubmed
    ..Comparison of the binding energies of subsites, which were calculated with a computer program, shows that BLA has similarity to the closely related Bacillus amyloliquefaciens alpha-amylase. ..
  5. Grossman M, Lampen J. Purification and DNA binding properties of the blaI gene product, repressor for the beta-lactamase gene, blaP, of Bacillus licheniformis. Nucleic Acids Res. 1987;15:6049-62 pubmed
    ..Binding of BlaI to DNA was detected by the slower migration of protein DNA complexes during polyacrylamide gel electrophoresis. BlaI was shown to selectively bind DNA fragments carrying the promoter regions of blaI and blaP. ..
  6. Hudspeth D, Vary P. spoVG sequence of Bacillus megaterium and Bacillus subtilis (Biochim. Biophys. Acta Vol. 1130, No. 2 (1992) 229-231 (BBAEXP 90336)). Biochim Biophys Acta. 1993;1216:509 pubmed
  7. Knox J, Moews P, Escobar W, Fink A. A catalytically-impaired class A beta-lactamase: 2 A crystal structure and kinetics of the Bacillus licheniformis E166A mutant. Protein Eng. 1993;6:11-8 pubmed
    ..The hydrolytic water seen in the native active site shifts markedly by 1.6 A, but is held in the active site by Asn170, which possibly becomes an ineffective substitute for Glu166 in activating the water for deacylation. ..
  8. Syed R, Wu Z, Hogle J, Hilvert D. Crystal structure of selenosubtilisin at 2.0-A resolution. Biochemistry. 1993;32:6157-64 pubmed
  9. Cha J, Stewart G. The divIVA minicell locus of Bacillus subtilis. J Bacteriol. 1997;179:1671-83 pubmed
    ..DivIVA also appears to be the interface between a sporulation component and MinCD, freeing up the polar septation sites for use during the asymmetric septation event of the sporulation process...

More Information

Publications112 found, 100 shown here

  1. Stoll V, Eger B, Hynes R, Martichonok V, Jones J, Pai E. Differences in binding modes of enantiomers of 1-acetamido boronic acid based protease inhibitors: crystal structures of gamma-chymotrypsin and subtilisin Carlsberg complexes. Biochemistry. 1998;37:451-62 pubmed publisher
  2. Lee J, Edwards C, Hulett F. Bacillus licheniformis APase I gene promoter: a strong well-regulated promoter in B. subtilis. J Gen Microbiol. 1991;137:1127-33 pubmed
    ..Heterologous expression studies of the promoter using a fusion with the lacZ gene indicated that it functions as a very strong inducible promoter in B. subtilis that is tightly regulated by phosphate concentration. ..
  3. Amory A, Kunst F, Aubert E, Klier A, Rapoport G. Characterization of the sacQ genes from Bacillus licheniformis and Bacillus subtilis. J Bacteriol. 1987;169:324-33 pubmed
    ..The target site of levansucrase regulation by sacQ was identified as a 440-base-pair fragment located in the 5' noncoding region of sacB, suggesting transcriptional control. ..
  4. Taylor P, Fotheringham I. Nucleotide sequence of the Bacillus licheniformis ATCC 10716 dat gene and comparison of the predicted amino acid sequence with those of other bacterial species. Biochim Biophys Acta. 1997;1350:38-40 pubmed
  5. Wray L, Fisher S. Functional analysis of the carboxy-terminal region of Bacillus subtilis TnrA, a MerR family protein. J Bacteriol. 2007;189:20-7 pubmed
    ..Surprisingly, gel filtration and cross-linking analysis showed that a truncated TnrA protein which contained only the N-terminal DNA binding domain was dimeric. The implications of these results for the structure of TnrA are discussed. ..
  6. Borch Pedersen K, Lindbäck T, Madslien E, Kidd S, O Sullivan K, Granum P, et al. The Cooperative and Interdependent Roles of GerA, GerK, and Ynd in Germination of Bacillus licheniformis Spores. Appl Environ Microbiol. 2016;82:4279-4287 pubmed publisher
    ..This study demonstrates the complex interactions between germinant receptors necessary for efficient germination of B. licheniformis spores. ..
  7. Jørgensen L, Hansen C, Poulsen G, Diderichsen B. In vivo genetic engineering: homologous recombination as a tool for plasmid construction. Gene. 1990;96:37-41 pubmed
    ..stearothermophilus, was created. The resulting hybrid translational product was processed correctly in B. subtilis during secretion, giving rise to an Amy identical to the mature Amy secreted by B. stearothermophilus. ..
  8. Zhu Y, Curran I, Joris B, Ghuysen J, Lampen J. Identification of BlaR, the signal transducer for beta-lactamase production in Bacillus licheniformis, as a penicillin-binding protein with strong homology to the OXA-2 beta-lactamase (class D) of Salmonella typhimurium. J Bacteriol. 1990;172:1137-41 pubmed
    ..The BlaR-beta-lactam complex is stable and may provide the continuing stimulus needed for the prolonged production of the enzyme. ..
  9. Izui K, Nielsen J, Caulfield M, Lampen J. Large exopenicillinase, initial extracellular form detected in cultures of Bacillus licheniformis. Biochemistry. 1980;19:1882-6 pubmed
  10. Brzozowski A, Lawson D, Turkenburg J, Bisgaard Frantzen H, Svendsen A, Borchert T, et al. Structural analysis of a chimeric bacterial alpha-amylase. High-resolution analysis of native and ligand complexes. Biochemistry. 2000;39:9099-107 pubmed
  11. Gray G, Mainzer S, Rey M, Lamsa M, Kindle K, Carmona C, et al. Structural genes encoding the thermophilic alpha-amylases of Bacillus stearothermophilus and Bacillus licheniformis. J Bacteriol. 1986;166:635-43 pubmed
    ..Several purified hybrid alpha-amylases were characterized and found to differ with respect to thermostability and specific activity. ..
  12. Himeno T, Imanaka T, Aiba S. Nucleotide sequence of the penicillinase repressor gene penI of Bacillus licheniformis and regulation of penP and penI by the repressor. J Bacteriol. 1986;168:1128-32 pubmed
    ..The regulation of penP and penI by the repressor is discussed. ..
  13. Fitzpatrick P, Steinmetz A, Ringe D, Klibanov A. Enzyme crystal structure in a neat organic solvent. Proc Natl Acad Sci U S A. 1993;90:8653-7 pubmed
    ..One-third of all subtilisin-bound acetonitrile molecules reside in the active center, occupying the same region (P1, P2, and P3 binding sites) as the specific protein inhibitor eglin c...
  14. Sievers J, Errington J. Analysis of the essential cell division gene ftsL of Bacillus subtilis by mutagenesis and heterologous complementation. J Bacteriol. 2000;182:5572-9 pubmed
    ..subtilis ftsL product) was not functional. We conclude that FtsL is a highly malleable protein that can accommodate a large number of sequence changes without loss of function. ..
  15. Ryttersgaard C, Le Nours J, Lo Leggio L, Jørgensen C, Christensen L, Bjørnvad M, et al. The structure of endo-beta-1,4-galactanase from Bacillus licheniformis in complex with two oligosaccharide products. J Mol Biol. 2004;341:107-17 pubmed
    ..This difference in substrate preference can be explained structurally by the presence of the extra subsites -3 and -4 in BLGAL. ..
  16. Liang W, Lin M, Chi M, Hu H, Lo H, Chang H, et al. Deletion analysis of the C-terminal region of a molecular chaperone DnaK from Bacillus licheniformis. Arch Microbiol. 2009;191:583-93 pubmed publisher
    ..These results suggest that C-terminal truncations alter the ATPase activity and thermal stability of BlDnaK and induce the conformation change of the ATPase domain. ..
  17. Lin M, Chi M, Chen B, Wang T, Lo H, Lin L. Residues Phe103 and Phe149 are critical for the co-chaperone activity of Bacillus licheniformis GrpE. Int J Biol Macromol. 2015;72:724-31 pubmed publisher
    ..14 kcal/mol. Taken together, the introduction of tryptophan residue, especially at positions 103 and 149, into the primary structure of BlGrpE has been proven to be detrimental to structural integrity and proper function of the protein. ..
  18. Lee S, Kim S, Sweet R, Suh S. Crystallization and a preliminary X-ray crystallographic study of alpha-amylase from Bacillus licheniformis. Arch Biochem Biophys. 1991;291:255-7 pubmed
    ..0 A Bragg spacing when exposed to synchrotron X-rays and they are reasonably stable in the X-ray beam. Thus the crystals are suitable for structure determination at high resolution by X-ray methods. ..
  19. Oda Y, Nakayama R, Kuroda A, Sekiguchi J. Molecular cloning, sequence analysis, and characterization of a new cell wall hydrolase, CwlL, of Bacillus licheniformis. Mol Gen Genet. 1993;241:380-8 pubmed
    ..subtilis amidase CwlA indicates two evolutionarily distinguishable regions in CwlL. The sequence homology of CwlL with other cell wall hydrolases and the regulation of cwlL are discussed. ..
  20. Akbar S, Kang C, Gaidenko T, Price C. Modulator protein RsbR regulates environmental signalling in the general stress pathway of Bacillus subtilis. Mol Microbiol. 1997;24:567-78 pubmed
    ..Our data further suggest that: (i) RsbR influences the antagonist function of RsbS by direct protein-protein interaction; and (ii) this interaction with RsbS is likely controlled by the phosphorylation state of RsbR...
  21. Fonzé E, Vanhove M, Dive G, Sauvage E, Frere J, Charlier P. Crystal structures of the Bacillus licheniformis BS3 class A beta-lactamase and of the acyl-enzyme adduct formed with cefoxitin. Biochemistry. 2002;41:1877-85 pubmed
  22. Furuya T, Takahashi S, Ishii Y, Kino K, Kirimura K. Cloning of a gene encoding flavin reductase coupling with dibenzothiophene monooxygenase through coexpression screening using indigo production as selective indication. Biochem Biophys Res Commun. 2004;313:570-5 pubmed
  23. Tschauder S, Driessen A, Freudl R. Cloning and molecular characterization of the secY genes from Bacillus licheniformis and Staphylococcus carnosus: comparative analysis of nine members of the SecY family. Mol Gen Genet. 1992;235:147-52 pubmed
    ..It is suggested that the conserved regions might be involved in the translocation activity of SecY or might be required for the correct interaction of SecY with other components of the secretion apparatus. ..
  24. Fujita Y, Shindo K, Miwa Y, Yoshida K. Bacillus subtilis inositol dehydrogenase-encoding gene (idh): sequence and expression in Escherichia coli. Gene. 1991;108:121-5 pubmed
    ..20% of total protein upon addition of isopropyl-beta-D-thiogalactopyranoside. This overproduced enzyme cross-reacted with an anti-Idh antibody, and exhibited the same Mr and substrate specificity as those of the B. subtilis enzyme. ..
  25. Scheler A, Rygus T, Allmansberger R, Hillen W. Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus licheniformis encoded regulon for xylose utilization. Arch Microbiol. 1991;155:526-34 pubmed
    ..The next greater differences are found to the S. xylosus and the greatest to the E. coli encoded genes. These results are discussed with respect to the taxonomic relations of these bacteria...
  26. McPhalen C, Schnebli H, James M. Crystal and molecular structure of the inhibitor eglin from leeches in complex with subtilisin Carlsberg. FEBS Lett. 1985;188:55-8 pubmed
    ..The structure of subtilisin Carlsberg in this complex is very similar to the known structure of subtilisin novo, despite changes of 84 out of 274 amino acids...
  27. Smith E, DeLange R, Evans W, Landon M, Markland F. Subtilisin Carlsberg. V. The complete sequence; comparison with subtilisin BPN'; evolutionary relationships. J Biol Chem. 1968;243:2184-91 pubmed
  28. Kuhn H, Fietzek P, Lampen J. N-terminal amino acid sequence of Bacillus licheniformis alpha-amylase: comparison with Bacillus amyloliquefaciens and Bacillus subtilis Enzymes. J Bacteriol. 1982;149:372-3 pubmed
    ..Their N-terminal amino acid sequences showed extensive homology with each other, but not with the saccharifying alpha-amylases of Bacillus subtilis. ..
  29. Oguro A, Kakeshita H, Takamatsu H, Nakamura K, Yamane K. The effect of Srb, a homologue of the mammalian SRP receptor alpha-subunit, on Bacillus subtilis growth and protein translocation. Gene. 1996;172:17-24 pubmed
  30. Kim J, Peterson T, Bee G, Hulett F. Bacillus licheniformis MC14 alkaline phosphatase I gene with an extended COOH-terminus. FEMS Microbiol Lett. 1998;159:47-58 pubmed
    ..licheniformis MC14. Another open reading frame of 261 amino acids was present 142 nucleotide upstream of the APaseI gene and its predicted amino acid sequence showed 68% identity to that of glucose dehydrogenase of B. megaterium. ..
  31. Schmitke J, Stern L, Klibanov A. Organic solvent binding to crystalline subtilisin1 in mostly aqueous media and in the neat solvents. Biochem Biophys Res Commun. 1998;248:273-7 pubmed
    ..Furthermore, the organic solvent molecules detected in the enzyme active site in the aqueous-organic mixtures are in the same locations as in the structures in the corresponding neat organic solvents. ..
  32. Zhu Y, Kobayashi T, Lampen J. A hypothetical protein (P20), homologous to Tn3 repressor is encoded downstream from the bla regulatory region in Bacillus licheniformis 749. Nucleic Acids Res. 1988;16:5691 pubmed
  33. Nicholls N, Lampen J. Repressor gene, blaI, for Bacillus licheniformis 749 beta-lactamase. FEBS Lett. 1987;221:179-83 pubmed
    ..Interaction with both promoters seemed necessary for full repression. BlaI is a hydrophilic protein (Mr 15036) with the some structural similarities to repressors from Gram-negative bacteria. ..
  34. Declerck N, Machius M, Joyet P, Wiegand G, Huber R, Gaillardin C. Hyperthermostabilization of Bacillus licheniformis alpha-amylase and modulation of its stability over a 50 degrees C temperature range. Protein Eng. 2003;16:287-93 pubmed
    ..Our engineering work on BLA shows that the thermostability of an already naturally highly thermostable enzyme can be substantially improved and modulated over a temperature range of 50 degrees C through a few point mutations...
  35. Lin L, Chen Y, Chi M, Merlino A. Low resolution X-ray structure of ?-glutamyltranspeptidase from Bacillus licheniformis: opened active site cleft and a cluster of acid residues potentially involved in the recognition of a metal ion. Biochim Biophys Acta. 2014;1844:1523-9 pubmed publisher
    ..The results show that replacement of Asp568 with an Ala affects both the autoprocessing and structural stability of the protein. ..
  36. Petsko G, Tsernoglou D. The structure of subtilopeptidase A. I. X-ray crystallographic data. J Mol Biol. 1976;106:453-6 pubmed
  37. Sekiguchi J, Ohsu H, Kuroda A, Moriyama H, Akamatsu T. Nucleotide sequences of the Bacillus subtilis flaD locus and a B. licheniformis homologue affecting the autolysin level and flagellation. J Gen Microbiol. 1990;136:1223-30 pubmed
    ..The regions 3' of the ORFs (flaL and flaD) in both species resemble rho-independent terminators of transcription. The characteristics of the amino acid sequences are also discussed. ..
  38. Schläpfer B, Portmann W, Branlant C, Branlant G, Zuber H. Nucleotide sequence of the glyceraldehyde-3-phosphate dehydrogenase from Bacillus megaterium. Nucleic Acids Res. 1990;18:6422 pubmed
  39. Neugebauer K, Sprengel R, Schaller H. Penicillinase from Bacillus licheniformis: nucleotide sequence of the gene and implications for the biosynthesis of a secretory protein in a Gram-positive bacterium. Nucleic Acids Res. 1981;9:2577-88 pubmed
    ..Of these 34 amino acids precede the sequence of the secreted form of the enzyme. This peptide extension shows the features of a signal for secretion and also provides the hydrophobic anchor for the membrane-bound form of the enzyme. ..
  40. Ramakrishna N, Dubnau E, Smith I. The complete DNA sequence and regulatory regions of the Bacillus licheniformis spoOH gene. Nucleic Acids Res. 1984;12:1779-90 pubmed
    ..coli-like promoter sequence, and is transcribed in vitro specifically only by RNA polymerase containing delta 55 and not delta 37-containing holoenzyme. ..
  41. Nagata S, Bakthavatsalam S, Galkin A, Asada H, Sakai S, Esaki N, et al. Gene cloning, purification, and characterization of thermostable and halophilic leucine dehydrogenase from a halophilic thermophile, Bacillus licheniformis TSN9. Appl Microbiol Biotechnol. 1995;44:432-8 pubmed
    ..However, they were markedly distinct from each other in halophilicity; the B. licheniformis enzyme was much more stable than the other in the presence of high concentrations of salts. ..
  42. Liu Y, Zhang J, Liu Q, Zhang C, Ma Q. Molecular cloning of novel cellulase genes cel9A and cel12A from Bacillus licheniformis GXN151 and synergism of their encoded polypeptides. Curr Microbiol. 2004;49:234-8 pubmed
    ..The combinations of Cel9A and Cel12A produced by the recombinant E. coli exhibited synergistic action against substrates of carboxylmethyl cellulose as well as Avicel. ..
  43. Liberato M, Silveira R, Prates Ã, de Araujo E, Pellegrini V, Camilo C, et al. Molecular characterization of a family 5 glycoside hydrolase suggests an induced-fit enzymatic mechanism. Sci Rep. 2016;6:23473 pubmed publisher
    ..This is the first example of a multidomain GH relying on large amplitude motions of the CBM46 for assembly of the catalytically competent form of the enzyme. ..
  44. Schmitke J, Stern L, Klibanov A. The crystal structure of subtilisin Carlsberg in anhydrous dioxane and its comparison with those in water and acetonitrile. Proc Natl Acad Sci U S A. 1997;94:4250-5 pubmed
    ..The locations of the bound solvent in the dioxane structure are distinct from those in the structures in acetonitrile and in water. ..
  45. Knox J, Moews P. Beta-lactamase of Bacillus licheniformis 749/C. Refinement at 2 A resolution and analysis of hydration. J Mol Biol. 1991;220:435-55 pubmed
    ..The position of the water-Glu166 pair is stabilized in the active site by a cis peptide bond at Pro167...
  46. Bode W, Papamokos E, Musil D. The high-resolution X-ray crystal structure of the complex formed between subtilisin Carlsberg and eglin c, an elastase inhibitor from the leech Hirudo medicinalis. Structural analysis, subtilisin structure and interface geometry. Eur J Biochem. 1987;166:673-92 pubmed
    ..Most of the intermolecular contacts are contributed by the nine residues of the reactive-site loop Gly40I-Arg48I.(ABSTRACT TRUNCATED AT 400 WORDS)..
  47. Declerck N, Joyet P, Trosset J, Garnier J, Gaillardin C. Hyperthermostable mutants of Bacillus licheniformis alpha-amylase: multiple amino acid replacements and molecular modelling. Protein Eng. 1995;8:1029-37 pubmed
    ..The model suggests that replacement by, for example, a valine could fill this cavity and therefore increase intra- and interhelical compactness and hydrophobic interactions...
  48. Brody M, Price C. Bacillus licheniformis sigB operon encoding the general stress transcription factor sigma B. Gene. 1998;212:111-8 pubmed
    ..Based on these results, B. licheniformis possesses a general stress system likely regulated by two coupled partner switching modules that sense and integrate the two broad classes of activating stress signals...
  49. Yakimov M, Kr ger A, Slepak T, Giuliano L, Timmis K, Golyshin P. A putative lichenysin A synthetase operon in Bacillus licheniformis: initial characterization. Biochim Biophys Acta. 1998;1399:141-53 pubmed
  50. Schmitke J, Stern L, Klibanov A. Comparison of x-ray crystal structures of an acyl-enzyme intermediate of subtilisin Carlsberg formed in anhydrous acetonitrile and in water. Proc Natl Acad Sci U S A. 1998;95:12918-23 pubmed
    ..The mechanistic and preparative implications of our findings for enzymatic catalysis in organic solvents are discussed...
  51. Rivera M, L pez Mungu a A, Sober n X, Saab Rinc n G. Alpha-amylase from Bacillus licheniformis mutants near to the catalytic site: effects on hydrolytic and transglycosylation activity. Protein Eng. 2003;16:505-14 pubmed
    ..The engineered enzyme reported here may represent an attractive alternative for the starch transformation industries as it affords direct and substantial material savings and requires no process modifications...
  52. Koumoutsi A, Chen X, Henne A, Liesegang H, Hitzeroth G, Franke P, et al. Structural and functional characterization of gene clusters directing nonribosomal synthesis of bioactive cyclic lipopeptides in Bacillus amyloliquefaciens strain FZB42. J Bacteriol. 2004;186:1084-96 pubmed
  53. Voigt B, J rgen B, Albrecht D, Ehrenreich A, Veith B, Evers S, et al. The glucose and nitrogen starvation response of Bacillus licheniformis. Proteomics. 2007;7:413-23 pubmed publisher
    ..Among them were yvyD, citA and the putative methylcitrate shunt genes mmgD, mmgE and yqiQ. However, both starvation conditions did not induce a general SigmaB-dependent stress response...
  54. Gondry M, Sauguet L, Belin P, Thai R, Amouroux R, Tellier C, et al. Cyclodipeptide synthases are a family of tRNA-dependent peptide bond-forming enzymes. Nat Chem Biol. 2009;5:414-20 pubmed publisher
    ..Therefore, AlbC and these related proteins belong to a newly defined family of enzymes that we have named cyclodipeptide synthases (CDPSs)...
  55. Svendsen I, Breddam K. Isolation and amino acid sequence of a glutamic acid specific endopeptidase from Bacillus licheniformis. Eur J Biochem. 1992;204:165-71 pubmed
    ..The enzyme is completely inhibited by diisopropylfluorophosphate, suggesting that it is a serine endopeptidase. It is partially inhibited by EDTA...
  56. Micka B, Groch N, Heinemann U, Marahiel M. Molecular cloning, nucleotide sequence, and characterization of the Bacillus subtilis gene encoding the DNA-binding protein HBsu. J Bacteriol. 1991;173:3191-8 pubmed
    ..In this strain (BM19), normal growth was found to depend on IPTG, whereas in the absence of IPTG, growth was severely affected. These results suggest an essential role for the hbs gene product for normal growth in B. subtilis...
  57. Declerck N, Joyet P, Gaillardin C, Masson J. Use of amber suppressors to investigate the thermostability of Bacillus licheniformis alpha-amylase. Amino acid replacements at 6 histidine residues reveal a critical position at His-133. J Biol Chem. 1990;265:15481-8 pubmed
    ..The advantages and limits of informational suppression in protein stability studies are discussed as well as structural features involved in the thermostability of B. licheniformis alpha-amylase...
  58. Yuuki T, Nomura T, Tezuka H, Tsuboi A, Yamagata H, Tsukagoshi N, et al. Complete nucleotide sequence of a gene coding for heat- and pH-stable alpha-amylase of Bacillus licheniformis: comparison of the amino acid sequences of three bacterial liquefying alpha-amylases deduced from the DNA sequences. J Biochem. 1985;98:1147-56 pubmed
    ..Nevertheless, several regions of the alpha-amylases appeared to be clearly distinct from one another when their hydropathy profiles were compared...
  59. Jacobs M, Eliasson M, Uhl n M, Flock J. Cloning, sequencing and expression of subtilisin Carlsberg from Bacillus licheniformis. Nucleic Acids Res. 1985;13:8913-26 pubmed
    ..Expression studies in Bacillus subtilis show that the cloned fragment produces a functional enzyme when inserted after a B. subtilis promoter...
  60. Kelly J, Dideberg O, Charlier P, Wery J, Libert M, Moews P, et al. On the origin of bacterial resistance to penicillin: comparison of a beta-lactamase and a penicillin target. Science. 1986;231:1429-31 pubmed
  61. Ledent P, Duez C, Vanhove M, Lejeune A, Fonz E, Charlier P, et al. Unexpected influence of a C-terminal-fused His-tag on the processing of an enzyme and on the kinetic and folding parameters. FEBS Lett. 1997;413:194-6 pubmed
    ..This resulted in the secretion of a protein with a different N-terminus, showing that this type of protein engineering might not always be as 'neutral' as generally assumed...
  62. Prange T, Schiltz M, Pernot L, Colloc h N, Longhi S, Bourguet W, et al. Exploring hydrophobic sites in proteins with xenon or krypton. Proteins. 1998;30:61-73 pubmed
    ..These xenon complexes can be used to map hydrophobic sites in proteins, or as heavy-atom derivatives in the isomorphous replacement method of structure determination...
  63. Oguro A, Kakeshita H, Nakamura K, Yamane K, Wang W, Bechhofer D. Bacillus subtilis RNase III cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor. J Biol Chem. 1998;273:19542-7 pubmed
    ..It is concluded that Bs-RNase III is an enzyme that processes pre-scRNA...
  64. Evans K, Crowder J, Miller E. Subtilisins of Bacillus spp. hydrolyze keratin and allow growth on feathers. Can J Microbiol. 2000;46:1004-11 pubmed
  65. Lapidus A, Galleron N, Andersen J, J rgensen P, Ehrlich S, Sorokin A. Co-linear scaffold of the Bacillus licheniformis and Bacillus subtilis genomes and its use to compare their competence genes. FEMS Microbiol Lett. 2002;209:23-30 pubmed
    ..A new insertion sequence, designated IS3Bli1, was discovered in the competence region of the analyzed B. licheniformis strain...
  66. Machius M, Declerck N, Huber R, Wiegand G. Kinetic stabilization of Bacillus licheniformis alpha-amylase through introduction of hydrophobic residues at the surface. J Biol Chem. 2003;278:11546-53 pubmed publisher
    ..At such positions, even the apparently thermodynamically unfavorable introduction of hydrophobic residues should be explored...
  67. Barrette Ng I, Ng K, Cherney M, Pearce G, Ryan C, James M. Structural basis of inhibition revealed by a 1:2 complex of the two-headed tomato inhibitor-II and subtilisin Carlsberg. J Biol Chem. 2003;278:24062-71 pubmed publisher
    ..subtilisin)2 complex provides a molecular framework for understanding how multiple inhibitory domains in a single Potato II type proteinase inhibitor molecule from the Potato II family act to inhibit proteolytic enzymes...
  68. Craynest M, J rgensen S, Sarvas M, Kontinen V. Enhanced secretion of heterologous cyclodextrin glycosyltransferase by a mutant of Bacillus licheniformis defective in the D-alanylation of teichoic acids. Lett Appl Microbiol. 2003;37:75-80 pubmed
    ..The results suggest that the d-alanylation of teichoic acids is an element that can be used to improve the production of some secretory proteins in industrial applications based on this important industrial microorganism...
  69. Horn J, Ramaswamy S, Murphy K. Structure and energetics of protein-protein interactions: the role of conformational heterogeneity in OMTKY3 binding to serine proteases. J Mol Biol. 2003;331:497-508 pubmed
    ..These effects emphasize that small, "rigid-body" proteins are still dynamic structures, and these dynamics make contributions to both the enthalpy and entropy of binding interactions...
  70. Berensmeier S, Singh S, Meens J, Buchholz K. Cloning of the pelA gene from Bacillus licheniformis 14A and biochemical characterization of recombinant, thermostable, high-alkaline pectate lyase. Appl Microbiol Biotechnol. 2004;64:560-7 pubmed publisher
    ..The yields of unsaturated trigalacturonic acids were further examined for the substrates polygalacturonic acid, citrus pectin and sugar-beet pectin...
  71. Gloster T, Ibatullin F, Macauley K, Ekl f J, Roberts S, Turkenburg J, et al. Characterization and three-dimensional structures of two distinct bacterial xyloglucanases from families GH5 and GH12. J Biol Chem. 2007;282:19177-89 pubmed publisher
    ..The differential strategies for the accommodation of the side chains of xyloglucan presumably facilitate the action of these microbial hydrolases in milieus where diverse and differently substituted substrates may be encountered...
  72. Boudet J, Duval V, Van Melckebeke H, Blackledge M, Amoroso A, Joris B, et al. Conformational and thermodynamic changes of the repressor/DNA operator complex upon monomerization shed new light on regulation mechanisms of bacterial resistance against beta-lactam antibiotics. Nucleic Acids Res. 2007;35:4384-95 pubmed publisher
    ..These results open new insights for the repression and induction mechanisms of bacterial resistance to beta-lactams...
  73. Lyu R, Hu H, Kuo L, Lo H, Ong P, Chang H, et al. Role of the conserved Thr399 and Thr417 residues of Bacillus licheniformis gamma-Glutamyltranspeptidase as evaluated by mutational analysis. Curr Microbiol. 2009;59:101-6 pubmed publisher
    ..These results suggest that residues Thr399 and Thr417 are important for BlGGT in the enzymatic maturation and reaction...
  74. Moews P, Knox J, Dideberg O, Charlier P, Fr re J. Beta-lactamase of Bacillus licheniformis 749/C at 2 A resolution. Proteins. 1990;7:156-71 pubmed publisher
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    ..Signal emission in the cytosol by the intracellular 189-amino-acid-residue Y134-K322 loop that connects TM3 and TM4, may proceed via the activation of a putative metallopeptidase...
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    ..The information presented here may help in the rational design of mutants with enhanced performance in biotechnological applications...
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    ..With their neutral pH optima and good temperature stabilities, these Bacillus phytases are good candidates for animal feed applications and transgenic studies...
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    ..Hence, such genes appear to be suitable disruption targets for achieving passive biological containment in this industrially exploited species...
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    ..A semiempirical model has been used for the prediction of saccharide composition of starch hydrolysate with respect to time...