Genomes and Genes
Bacillus cereus ATCC 14579
Alias: Bacillus cereus (strain ATCC 14579 / DSM 31)
Publications154 found, 100 shown here
- van Schaik W, van der Voort M, Molenaar D, Moezelaar R, de Vos W, Abee T. Identification of the sigmaB regulon of Bacillus cereus and conservation of sigmaB-regulated genes in low-GC-content gram-positive bacteria. J Bacteriol. 2007;189:4384-90 pubmed
- Declerck N, Bouillaut L, Chaix D, Rugani N, Slamti L, Hoh F, et al. Structure of PlcR: Insights into virulence regulation and evolution of quorum sensing in Gram-positive bacteria. Proc Natl Acad Sci U S A. 2007;104:18490-5 pubmed..Our results shed light on virulence control by PlcR and elucidate origin and evolution of multicellular behavior in bacteria. ..
- van der Voort M, Kuipers O, Buist G, de Vos W, Abee T. Assessment of CcpA-mediated catabolite control of gene expression in Bacillus cereus ATCC 14579. BMC Microbiol. 2008;8:62 pubmed publisher..Here we studied the role of Bacillus cereus ATCC 14579 CcpA in regulation of metabolic pathways and expression of enterotoxin genes by comparative ..
- Gohar M, Faegri K, Perchat S, Ravnum S, Økstad O, Gominet M, et al. The PlcR virulence regulon of Bacillus cereus. PLoS ONE. 2008;3:e2793 pubmed publisher..cereus growth within the host: food supply, host barriers, host immune defenses, and competition with other bacterial species. PlcR appears to be a key component in the efficient adaptation of B. cereus to its host environment. ..
- Brillard J, Susanna K, Michaud C, Dargaignaratz C, Gohar M, Nielsen Leroux C, et al. The YvfTU two-component system is involved in plcR expression in Bacillus cereus. BMC Microbiol. 2008;8:183 pubmed publisher..cereus group, the plcR gene is always located in the vicinity of genes encoding the YvfTU two-component system. The putative role of YvfTU in the expression of the PlcR regulon was therefore investigated...
- Landete J, Arena M, Pardo I, Manca de Nadra M, Ferrer S. The role of two families of bacterial enzymes in putrescine synthesis from agmatine via agmatine deiminase. Int Microbiol. 2010;13:169-77 pubmed..linked to the aguA and ptcA genes in Lactobacillus hilgardii X1B, Enterococcus faecalis ATCC 11700, and Bacillus cereus ATCC 14579. By contrast Lactobacillus sakei 23K was unable to produce putrescine, and although a fragment of DNA ..
- Hwang S, Li Z, Bar Peled Y, Aronov A, Ericson J, Bar Peled M. The biosynthesis of UDP-d-FucNAc-4N-(2)-oxoglutarate (UDP-Yelosamine) in Bacillus cereus ATCC 14579: Pat and Pyl, an aminotransferase and an ATP-dependent Grasp protein that ligates 2-oxoglutarate to UDP-4-amino-sugars. J Biol Chem. 2014;289:35620-32 pubmed publisher
- Han H, Sullivan T, Wilson A. Cytochrome c551 and the cytochrome c maturation pathway affect virulence gene expression in Bacillus cereus ATCC 14579. J Bacteriol. 2015;197:626-35 pubmed publisher..These findings suggest a new mode of regulation of B. cereus virulence and reveal intriguing similarities and differences in virulence regulation between B. cereus and B. anthracis. ..
- Warda A, den Besten H, Sha N, Abee T, Nierop Groot M. Influence of food matrix on outgrowth heterogeneity of heat damaged Bacillus cereus spores. Int J Food Microbiol. 2015;201:27-34 pubmed publisher..In the current communication we studied the outgrowth from heat damaged Bacillus cereus ATCC 14579 spores on Anopore membrane, which allowed following outgrowth heterogeneity of individual spores on ..
- Madeira J, Omer H, Alpha Bazin B, Armengaud J, Duport C. Deciphering the interactions between the Bacillus cereus linear plasmid, pBClin15, and its host by high-throughput comparative proteomics. J Proteomics. 2016;146:25-33 pubmed publisher..The reference strain, Bacillus cereus ATCC 14579, harbors a linear plasmid, pBClin15, which displays a cryptic prophage behavior...
- Mezes P, Yang Y, Hussain M, Lampen J. Bacillus cereus 569/H beta-lactamase I: cloning in Escherichia coli and signal sequence determination. FEBS Lett. 1983;161:195-200 pubmed..cereus beta-lactamase I signal sequence is unmodified and a single processed form of the enzyme is present in E. coli cells carrying penPC. ..
- Moriyama R, Kudoh S, Miyata S, Nonobe S, Hattori A, Makino S. A germination-specific spore cortex-lytic enzyme from Bacillus cereus spores: cloning and sequencing of the gene and molecular characterization of the enzyme. J Bacteriol. 1996;178:5330-2 pubmed
- Shi X, Rao N, Kornberg A. Inorganic polyphosphate in Bacillus cereus: motility, biofilm formation, and sporulation. Proc Natl Acad Sci U S A. 2004;101:17061-5 pubmed publisher..These enzymes and genes in B. cereus are nearly identical to those in the very closely related pathogen Bacillus anthracis, and, thus, they may provide attractive targets for the treatment of anthrax...
- Røhr A, Hammerstad M, Andersson K. Tuning of thioredoxin redox properties by intramolecular hydrogen bonds. PLoS ONE. 2013;8:e69411 pubmed publisher..We suggest that our model applies to other thioredoxin subclasses with similar active site arrangements. ..
- Tagawa Y. Isolation and characterization of flagellar filaments from Bacillus cereus ATCC 14579. Antonie Van Leeuwenhoek. 2014;106:1157-65 pubmed publisher..cereus strains in possessing multiple genes that encode flagellin subunits, a possible unique mechanism may contribute to assembly of multiple flagellin subunits into the filament over its entire length. ..
- Kihara T, Hiroe A, Ishii Hyakutake M, Mizuno K, Tsuge T. Bacillus cereus-type polyhydroxyalkanoate biosynthetic gene cluster contains R-specific enoyl-CoA hydratase gene. Biosci Biotechnol Biochem. 2017;81:1627-1635 pubmed publisher..coli; however, it did not function when the phaJYB4 gene was deleted. The B. cereus-type pha cluster represents a new example of a pha cluster that contains the gene encoding PhaJ. ..
- Tomita M, Nakai K, Yamada A, Taguchi R, Ikezawa H. Secondary structure of sphingomyelinase from Bacillus cereus. J Biochem. 1990;108:811-5 pubmed..The partial homologies between the amino acid sequences of SMPLC and Clostridium perfringens alpha-toxin (phospholipase C) are discussed. ..
- Henner D, Yang M, Chen E, Hellmiss R, Rodriguez H, Low M. Sequence of the Bacillus thuringiensis phosphatidylinositol specific phospholipase C. Nucleic Acids Res. 1988;16:10383 pubmed
- Adachi W, Sakihama Y, Shimizu S, Sunami T, Fukazawa T, Suzuki M, et al. Crystal structure of family GH-8 chitosanase with subclass II specificity from Bacillus sp. K17. J Mol Biol. 2004;343:785-95 pubmed publisher..These structural features indicate that enzymes in GH-8 can be further divided into three subfamilies. Different types of chitosanases are discussed in terms of convergent evolution from different structural ancestors...
- Lechner M, Kupke T, Stefanovic S, Gotz F. Molecular characterization and sequence of phosphatidylinositol-specific phospholipase C of Bacillus thuringiensis. Mol Microbiol. 1989;3:621-6 pubmed..The enzyme from the E. coli recombinant shows no activity on other phospholipids and sphingomyelin. The cleaving specificity of PI-PLC was examined by thin layer chromatography. ..
- Sidler W, Niederer E, Suter F, Zuber H. The primary structure of Bacillus cereus neutral proteinase and comparison with thermolysin and Bacillus subtilis neutral proteinase. Biol Chem Hoppe Seyler. 1986;367:643-57 pubmed
- Hendrickx A, Poor C, Jureller J, Budzik J, He C, Schneewind O. Isopeptide bonds of the major pilin protein BcpA influence pilus structure and bundle formation on the surface of Bacillus cereus. Mol Microbiol. 2012;85:152-63 pubmed publisher..Thus, structural integrity of the CNA(1) and XNA domains are determinants for the association of pili into higher order bundle structures and determine native pilus structure. ..
- Fedhila S, Guillemet E, Nel P, Lereclus D. Characterization of two Bacillus thuringiensis genes identified by in vivo screening of virulence factors. Appl Environ Microbiol. 2004;70:4784-91 pubmed..Overall, our findings suggest that the yqgB and yqfZ genes encode adaptive factors that may act in synergy, enabling the bacteria to cope with the physical environment in vivo, facilitating colonization of the host. ..
- Driss F, Kallassy Awad M, Zouari N, Jaoua S. Molecular characterization of a novel chitinase from Bacillus thuringiensis subsp. kurstaki. J Appl Microbiol. 2005;99:945-53 pubmed..coli. The addition of the sequence of chi255 to the few sequenced B. thuringiensis chi genes might contribute to a better investigation of the chitinase 'structure-function' relation. ..
- Rather M, Aulakh R, Gill J, Mir A, Hassan M. Detection and sequencing of plasmid encoded tetracycline resistance determinants (tetA and tetB) from food-borne Bacillus cereus isolates. Asian Pac J Trop Med. 2012;5:709-12 pubmed publisher..cereus indicate the horizontal transfer of antibiotic resistance determinants from other bacteria into B. cereus. The transfer of these resistant determinants to other potentially pathogenic bacteria may be a matter of great concern. ..
- Yutsudo T, Okumura K, Iwasaki M, Hara A, Kamitani S, Minamide W, et al. The gene encoding a new mitogenic factor in a Streptococcus pyogenes strain is distributed only in group A streptococci. Infect Immun. 1994;62:4000-4 pubmed..These results indicate that mf is distributed specifically in group A streptococci and the presence of mf in clinical samples strongly suggests infection with group A streptococci. ..
- Davies A, Rasia R, Vila A, Sutton B, Fabiane S. Effect of pH on the active site of an Arg121Cys mutant of the metallo-beta-lactamase from Bacillus cereus: implications for the enzyme mechanism. Biochemistry. 2005;44:4841-9 pubmed publisher..5. This provides experimental evidence for an earlier proposed mechanism in which protonation of Asp120 and the Zn1-bound hydroxide are the two events that lead to the loss of activity at low pH...
- Str msten N, Benson S, Burnett R, Bamford D, Bamford J. The Bacillus thuringiensis linear double-stranded DNA phage Bam35, which is highly similar to the Bacillus cereus linear plasmid pBClin15, has a prophage state. J Bacteriol. 2003;185:6985-9 pubmed..1-kbp linear plasmid, pBClin15. We show that pBClin15 closely resembles Bam35 and demonstrate conversion of Bam35 to a prophage. This state is common, as several B. thuringiensis strains release Bam35-related viruses...
- Taylor Creel K, Hames M, Holloway W, McFeeters H, McFeeters R. Expression, purification, and solubility optimization of peptidyl-tRNA hydrolase 1 from Bacillus cereus. Protein Expr Purif. 2014;95:259-64 pubmed publisher..Enzymatic activity of Pth1 from B. cereus was confirmed and quantification of Michaelis-Menten parameters reported. ..
- Johansen T, Holm T, Guddal P, Sletten K, Haugli F, Little C. Cloning and sequencing of the gene encoding the phosphatidylcholine-preferring phospholipase C of Bacillus cereus. Gene. 1988;65:293-304 pubmed..coli. The cloning and sequencing described here complete the first step toward using in vitro mutagenesis for investigations of the structure-function relationships of B. cereus phospholipase C. ..
- Montagner C, Nigen M, Jacquin O, Willet N, Dumoulin M, Karsisiotis A, et al. The Role of Active Site Flexible Loops in Catalysis and of Zinc in Conformational Stability of Bacillus cereus 569/H/9 ?-Lactamase. J Biol Chem. 2016;291:16124-37 pubmed publisher..4 kJ·mol(-1) These combined data highlight the importance of the two zinc ions in maintaining structure as well as a relatively well defined conformation for both active site loops to maintain enzymatic activity. ..
- Lovgren A, Zhang M, Engstrom A, Landén R. Identification of two expressed flagellin genes in the insect pathogen Bacillus thuringiensis subsp. alesti. J Gen Microbiol. 1993;139:21-30 pubmed..DNA sequences related to the flaB gene were found in most other B. thuringiensis subspecies, and in two of them, subsp. kurstaki and subsp. entomocidus, such sequences were present in multiple copies. ..
- Siehl D, Castle L, Gorton R, Keenan R. The molecular basis of glyphosate resistance by an optimized microbial acetyltransferase. J Biol Chem. 2007;282:11446-55 pubmed publisher..The large improvement in kcat is likely because of the cooperative effects of additional substitutions located distal to the active site...
- Gassler C, Ryan M, Liu T, Griffith O, Heinz D. Probing the roles of active site residues in phosphatidylinositol-specific phospholipase C from Bacillus cereus by site-directed mutagenesis. Biochemistry. 1997;36:12802-13 pubmed
- Abi Khalil E, Segond D, Terpstra T, AndrÃ© Leroux G, Kallassy M, Lereclus D, et al. Heme interplay between IlsA and IsdC: Two structurally different surface proteins from Bacillus cereus. Biochim Biophys Acta. 2015;1850:1930-41 pubmed publisher..cereus are revealed. ..
- Zhang M, Lovgren A. Cloning and sequencing of a beta-lactamase-encoding gene from the insect pathogen Bacillus thuringiensis. Gene. 1995;158:83-6 pubmed
- Golden B, Hoffman D, Ramakrishnan V, White S. Ribosomal protein S17: characterization of the three-dimensional structure by 1H and 15N NMR. Biochemistry. 1993;32:12812-20 pubmed..Since these potential RNA-binding sites are distributed over a large surface of the protein, it appears that the molecule may interact with several regions of 16S rRNA...
- Shao C, Shi X, Wehbi H, Zambonelli C, Head J, Seaton B, et al. Dimer structure of an interfacially impaired phosphatidylinositol-specific phospholipase C. J Biol Chem. 2007;282:9228-35 pubmed..The combined data suggest that wild-type PI-PLC can form similar homodimers, anchored to the interface by the tryptophan and neighboring membrane-partitioning residues. ..
- Mayr B, Kaplan T, Lechner S, Scherer S. Identification and purification of a family of dimeric major cold shock protein homologs from the psychrotrophic Bacillus cereus WSBC 10201. J Bacteriol. 1996;178:2916-25 pubmed..At higher salt concentrations, they dissociate into their monomers. Their ability to bind to the ATTGG motif of single-stranded oligonucleotides was demonstrated by band shift analysis. ..
- Pauptit R, Karlsson R, Picot D, Jenkins J, Niklaus Reimer A, Jansonius J. Crystal structure of neutral protease from Bacillus cereus refined at 3.0 A resolution and comparison with the homologous but more thermostable enzyme thermolysin. J Mol Biol. 1988;199:525-37 pubmed..Other factors that may affect thermostability include the two glycine to alanine exchanges and perturbations in the environment of the double calcium site. ..
- Mezes P, Blacher R, Lampen J. Processing of Bacillus cereus 569/H beta-lactamase I in Escherichia coli and Bacillus subtilis. J Biol Chem. 1985;260:1218-23 pubmed..cereus 569/H indicated that UUG is being utilized as the initiation codon for penPC. The same result was obtained for the pre-beta-lactamase I from similarly treated cells of the closely related B. cereus 5/B strain. ..
- Gavrilenko I, Baida G, Karpov A, Kuz min N. [Nucleotide sequence of phospholipase C and sphingomyelinase genes from Bacillus cereus BKM-B164]. Bioorg Khim. 1993;19:133-8 pubmed
- Contreras Zentella M, Mendoza G, Membrillo Hernández J, Escamilla J. A novel double heme substitution produces a functional bo3 variant of the quinol oxidase aa3 of Bacillus cereus. Purification and paratial characterization. J Biol Chem. 2003;278:31473-8 pubmed..This is the first reported example of an enzymatically active promiscuous oxidase resulting from the simultaneous substitution of its original hemes in the high and low spin sites. ..
- Yaguchi M, Wittmann H, Cabezon T, Dewilde M, Villarroel R, Herzog A, et al. Alteration of ribosomal protein S17 by mutation linked to neamine resistance in Escherichia coli. II. Localization of the amino acid replacement in protein S17 from a meaA mutant. J Mol Biol. 1976;104:617-20 pubmed
- Ambler R, Daniel M, Fleming J, Hermoso J, Pang C, Waley S. The amino acid sequence of the zinc-requiring beta-lactamase II from the bacterium Bacillus cereus 569. FEBS Lett. 1985;189:207-11 pubmed
- Vis H, Mariani M, Vorgias C, Wilson K, Kaptein R, Boelens R. Solution structure of the HU protein from Bacillus stearothermophilus. J Mol Biol. 1995;254:692-703 pubmed..m.s.d. of 1.0 A calculated over the backbone heavy atoms of residues 54 to 59 and 69 to 74. The tip of the arm adopts a well-defined 4:6 beta-hairpin conformation similar to the iron co-ordinating beta-arms of rubredoxin. ..
- Morais M, Baker A, Dunaway Mariano D, Allen K. Crystallization and preliminary crystallographic analysis of phosphonoacetaldehyde hydrolase. Acta Crystallogr D Biol Crystallogr. 2000;56:206-9 pubmed..The combined phase set was further improved by solvent flattening, histogram matching and NCS averaging. Interpretation of the resulting electron-density map is currently under way. ..
- Thorsen L, Hansen B, Nielsen K, Hendriksen N, Phipps R, Budde B. Characterization of emetic Bacillus weihenstephanensis, a new cereulide-producing bacterium. Appl Environ Microbiol. 2006;72:5118-21 pubmed publisher..The strains are atypical with regard to pheno- and genotypic characteristics normally used for identification of emetic B. cereus strains. MC67 and MC118 produced cereulide at temperatures of as low as 8 degrees C...
- Broussolle V, Pandiani F, Haddad N, Michaud C, Carlin F, Nguyen the C, et al. Insertional mutagenesis reveals genes involved in Bacillus cereus ATCC 14579 growth at low temperature. FEMS Microbiol Lett. 2010;306:177-83 pubmed publisherTransposon mutagenesis of Bacillus cereus ATCC 14579 yielded cold-sensitive mutants...
- Kuppe A, Evans L, McMillen D, Griffith O. Phosphatidylinositol-specific phospholipase C of Bacillus cereus: cloning, sequencing, and relationship to other phospholipases. J Bacteriol. 1989;171:6077-83 pubmed..cereus PI-PLC contains a stretch of significant similarity to the glycosylphosphatidylinositol-specific PLC of Trypanosoma brucei. The conserved peptide is proposed to play a role in the function of these enzymes. ..
- Sun D, Setlow P. Cloning and nucleotide sequencing of genes for a second type of small, acid-soluble spore proteins of Bacillus cereus, Bacillus stearothermophilus, and "Thermoactinomyces thalpophilus". J Bacteriol. 1987;169:3088-93 pubmed..However, all five B-type SASP did contain a large (27 to 35-residue), rather well-conserved amino acid sequence repeat, and four of the five proteins had well-conserved regions of 14 to 17 amino acids which appeared three times. ..
- Vis H, Boelens R, Mariani M, Stroop R, Vorgias C, Wilson K, et al. 1H, 13C, and 15N resonance assignments and secondary structure analysis of the HU protein from Bacillus stearothermophilus using two- and three-dimensional double- and triple-resonance heteronuclear magnetic resonance spectroscopy. Biochemistry. 1994;33:14858-70 pubmed..The NOE data indicate further that the loop region in the DNA-binding arms of HU is arranged as a type I beta-turn from Pro-63 to Gly-66...
- Miyamoto T, Sayed M, Sasahara R, Sukimoto K, Umezaki A, Honjoh K, et al. Cloning and overexpression of Bacillus cereus penicillin-binding protein 3 gene in Escherichia coli. Biosci Biotechnol Biochem. 2002;66:44-50 pubmed..A protein was produced by the cells of E. coli carrying pET-pbp3. The produced protein migrated at about 75 kDa in SDS-polyacrylamide gel and strongly reacted with biotinylated ampicillin. ..
- Waterman D, Ortiz Lombard a M, Fogg M, Koonin E, Antson A. Crystal structure of Bacillus anthracis ThiI, a tRNA-modifying enzyme containing the predicted RNA-binding THUMP domain. J Mol Biol. 2006;356:97-110 pubmed publisher..The inaccessibility of U8 in the canonical L-shaped form of tRNA, and the existence of a glycine-rich linker joining the catalytic and RNA-binding moieties of ThiI suggest that structural changes may occur in both molecules upon binding...
- Zigha A, Rosenfeld E, Schmitt P, Duport C. Anaerobic cells of Bacillus cereus F4430/73 respond to low oxidoreduction potential by metabolic readjustments and activation of enterotoxin expression. Arch Microbiol. 2006;185:222-33 pubmed..This control was complex, involving different levels of regulation. We discussed the regulation of enterotoxin expression and the involvement of the pleiotropic regulator PlcR. ..
- Chazarreta Cifré L, Alemany M, de Mendoza D, Altabe S. Exploring the biosynthesis of unsaturated fatty acids in Bacillus cereus ATCC 14579 and functional characterization of novel acyl-lipid desaturases. Appl Environ Microbiol. 2013;79:6271-9 pubmed publisher..This represents the first work reporting the functional characterization of fatty acid desaturases from B. cereus. ..
- Wang W, Mezes P, Yang Y, Blacher R, Lampen J. Cloning and sequencing of the beta-lactamase I gene of Bacillus cereus 5/B and its expression in Bacillus subtilis. J Bacteriol. 1985;163:487-92 pubmed..subtilis BD170(pRWY215) is His-44, which is the same as the NH2 terminus of the major 569/H product from B. subtilis BD170(pRWM5). ..
- Loshon C, Beary K, Chander M, Setlow P. Cloning and sequencing of the sspF (originally 0.3 kb) genes from Bacillus cereus and Bacillus megaterium. Gene. 1994;150:203-4 pubmed..subtilis SspF. These proteins exhibit an average of 74% sequence identity across species, suggesting they may play some important role in either sporulation or the dormant spore. ..
- Stoyan T, Recktenwald A, Kula M. Cloning, sequencing and overexpression of the leucine dehydrogenase gene from Bacillus cereus. J Biotechnol. 1997;54:77-80 pubmed..stearothermophilus and Thermoactinomyces intermedius. The active protein was overexpressed in Escherichia coli to yield approximately 30% of the total soluble protein. ..
- Antikainen N, Monzingo A, Franklin C, Robertus J, Martin S. Using X-ray crystallography of the Asp55Asn mutant of the phosphatidylcholine-preferring phospholipase C from Bacillus cereus to support the mechanistic role of Asp55 as the general base. Arch Biochem Biophys. 2003;417:81-6 pubmed..These observations demonstrate that Asp55 is not critical for the structural integrity of the enzyme and support the hypothesis that Asp55 is the general base in the PLC(Bc)-catalyzed hydrolysis of phospholipids. ..
- Lister S, Wetmore D, Roche R, Codding P. E144S active-site mutant of the Bacillus cereus thermolysin-like neutral protease at 2.8 A resolution. Acta Crystallogr D Biol Crystallogr. 1996;52:543-50 pubmed..Res. 21, 333-340]. We suggest that the residual activity of the E144S mutant arises from a water molecule, which is found within hydrogen-bonding distance of Ser144, acting as a general base in the catalytic function of the mutant. ..
- Malumbres M, Mateos L, Guerrero C, Martin J. Nucleotide sequence of the threonine synthase (thrC) gene of Brevibacterium lactofermentum. Nucleic Acids Res. 1988;16:9859 pubmed
- Malumbres M, Mateos L, Guerrero C, Martin J. Molecular cloning of the hom-thrC-thrB cluster from Bacillus sp. ULM1: expression of the thrC gene in Escherichia coli and corynebacteria, and evolutionary relationships of the threonine genes. Folia Microbiol (Praha). 1995;40:595-606 pubmed..Amino acid comparison of nine threonine synthases revealed evolutionary relationships between different groups of bacteria. ..
- Ankarloo J, Zhang M, Lovgren A. Regulatory sequences of two flagellin genes in Bacillus thuringiensis subsp. alesti. Microbiology. 1996;142 ( Pt 2):315-20 pubmed..Both flaA and flaB were found to be transcribed from sigma70-like promoters. In addition, the 3'-terminal half of flaA was cloned and sequenced, completing the sequence of flaA. ..
- Panosian T, Nannemann D, Bachmann B, Iverson T. Crystallization and preliminary X-ray analysis of a phosphopentomutase from Bacillus cereus. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010;66:811-4 pubmed publisher..These results suggest that even if the catalytically relevant metal is not known, the crystallization of putative metalloproteins may still benefit from supplementation of the crystallization screens with potential catalytic metals. ..
- Hansen S, Hough E, Svensson L, Wong Y, Martin S. Crystal structure of phospholipase C from Bacillus cereus complexed with a substrate analog. J Mol Biol. 1993;234:179-87 pubmed..A water molecule, located approximately apical to the diacylglycerol leaving group, seems to be the most likely candidate for the attacking nucleophile which initiates the reaction. ..
- Ago H, Oda M, Takahashi M, Tsuge H, Ochi S, Katunuma N, et al. Structural basis of the sphingomyelin phosphodiesterase activity in neutral sphingomyelinase from Bacillus cereus. J Biol Chem. 2006;281:16157-67 pubmed publisher..In addition, the structural features and site-directed mutagenesis suggest that the specific beta-hairpin with the aromatic amino acid residues participates in binding to the membrane-bound sphingomyelin substrate...
- Satoh Y, Minamoto N, Tajima K, Munekata M. Polyhydroxyalkanoate synthase from Bacillus sp. INT005 is composed of PhaC and PhaR. J Biosci Bioeng. 2002;94:343-50 pubmed..Furthermore, the PHA synthase has no lag phase. We hence concluded that the PHA synthase of Bacillus sp. INT005 consists of PhaC and PhaR, and has characteristics different from class III PHA synthase. ..
- Herzog A, Yaguchi M, Cabez n T, Corchuelo M, Petre J, Bollen A. A missense mutation in the gene coding for ribosomal protein S17 (rpsQ) leading to ribosomal assembly defectivity in Escherichia coli. Mol Gen Genet. 1979;171:15-22 pubmed..We conclude therefore that the 2861 mis mutation affects the structural gene for protein S 17 (rpsQ)...
- Yoshisue H, Ihara K, Nishimoto T, Sakai H, Komano T. Cloning and characterization of a Bacillus thuringiensis homolog of the spoIIID gene from Bacillus subtilis. Gene. 1995;154:23-9 pubmed..These results strongly suggest that the gene we have cloned is a Bt homolog of spoIIID...
- Baida G, Budarina Z, Kuzmin N, Solonin A. Complete nucleotide sequence and molecular characterization of hemolysin II gene from Bacillus cereus. FEMS Microbiol Lett. 1999;180:7-14 pubmed..The data suggest that hemolysin II belongs to the group of beta-channel forming cytolysins...
- Espinasse S, Gohar M, Lereclus D, Sanchis V. An ABC transporter from Bacillus thuringiensis is essential for beta-exotoxin I production. J Bacteriol. 2002;184:5848-54 pubmed..thuringiensis adds an adenine nucleotide analog to the wide range of substrates of the superfamily of ABC proteins. We suggest that berAB confers beta-exotoxin I immunity in B. thuringiensis, through active efflux of the molecule...
- Pandiani F, Brillard J, Bornard I, Michaud C, Chamot S, Nguyen the C, et al. Differential involvement of the five RNA helicases in adaptation of Bacillus cereus ATCC 14579 to low growth temperatures. Appl Environ Microbiol. 2010;76:6692-7 pubmed publisherb>Bacillus cereus ATCC 14579 possesses five RNA helicase-encoding genes overexpressed under cold growth conditions. Out of the five corresponding mutants, only the ?cshA, ?cshB, and ?cshC strains were cold sensitive...
- Hansen S, Hansen L, Hough E. Crystal structures of phosphate, iodide and iodate-inhibited phospholipase C from Bacillus cereus and structural investigations of the binding of reaction products and a substrate analogue. J Mol Biol. 1992;225:543-9 pubmed..The ninth blocks the entrance to the active site cleft and is of higher occupancy. Altogether, these results suggest that the substrate, a phospholipid, is associated directly with the metal ions during catalysis...
- White S, Appelt K, Wilson K, Tanaka I. A protein structural motif that bends DNA. Proteins. 1989;5:281-8 pubmed publisher..From the high-resolution structure of HU, we propose a model for this interaction with DNA. Crucial amino acid differences between the proteins can be rationalized in terms of their different specific functions...
- Loshon C, Fliss E, Setlow B, Foerster H, Setlow P. Cloning and nucleotide sequencing of genes for small, acid-soluble spore proteins of Bacillus cereus, Bacillus stearothermophilus, and "Thermoactinomyces thalpophilus". J Bacteriol. 1986;167:168-73 pubmed..thalpophilus." This finding strongly suggests that there is significant selective pressure to conserve SASP primary sequence and thus that these proteins serve some function other than simply amino acid storage...
- Donovan W, Tan Y, Slaney A. Cloning of the nprA gene for neutral protease A of Bacillus thuringiensis and effect of in vivo deletion of nprA on insecticidal crystal protein. Appl Environ Microbiol. 1997;63:2311-7 pubmed..thuringiensis. These results indicate that crystal protein stability and yield may be improved by deletion of specific proteases from B. thuringiensis...
- Moriyama R, Fukuoka H, Miyata S, Kudoh S, Hattori A, Kozuka S, et al. Expression of a germination-specific amidase, SleB, of Bacilli in the forespore compartment of sporulating cells and its localization on the exterior side of the cortex in dormant spores. J Bacteriol. 1999;181:2373-8 pubmed..The peripheral location of the spore-lytic enzymes in the dormant spore suggests that spore germination is initiated at the exterior of the cortex...
- Granum P, O SULLIVAN K, Lund T. The sequence of the non-haemolytic enterotoxin operon from Bacillus cereus. FEMS Microbiol Lett. 1999;177:225-9 pubmed..There is substantial similarity between the three proteins of non-haemolytic enterotoxin and between the non-haemolytic enterotoxin and haemolytic enterotoxin proteins...
- Gabashvili I, Agrawal R, Spahn C, Grassucci R, Svergun D, Frank J, et al. Solution structure of the E. coli 70S ribosome at 11.5 A resolution. Cell. 2000;100:537-49 pubmed..Analysis of the bridging connections between the subunits provides insight into the dynamic signaling mechanism between the ribosomal subunits...
- Maruyama R, Nishizawa M, Itoi Y, Ito S, Inoue M. Isolation and expression of a Bacillus cereus gene encoding benzil reductase. Biotechnol Bioeng. 2001;75:630-3 pubmed
- Rasia R, Vila A. Exploring the role and the binding affinity of a second zinc equivalent in B. cereus metallo-beta-lactamase. Biochemistry. 2002;41:1853-60 pubmed..In addition, it is demonstrated that Arg121 is not responsible for the low binding affinity of betaLII toward a second zinc(II) equivalent...
- Miles G, Bayley H, Cheley S. Properties of Bacillus cereus hemolysin II: a heptameric transmembrane pore. Protein Sci. 2002;11:1813-24 pubmed publisher..Although they share limited primary sequence identity (30%), these data confirm that HlyII is a structural and functional homolog of staphylococcal alpha-hemolysin...
- Todd S, Moir A, Johnson M, Moir A. Genes of Bacillus cereus and Bacillus anthracis encoding proteins of the exosporium. J Bacteriol. 2003;185:3373-8 pubmed..Two enzymes, alanine racemase and nucleoside hydrolase, are tightly adsorbed to the exosporium layer; they could metabolize small molecule germinants and may reduce the sensitivity of spores to these, limiting premature germination...