Sinorhizobium meliloti 1021

Summary

Alias: Sinorhizobium meliloti str. 1021, Sinorhizobium meliloti strain 1021

Top Publications

  1. Reed J, Glazebrook J, Walker G. The exoR gene of Rhizobium meliloti affects RNA levels of other exo genes but lacks homology to known transcriptional regulators. J Bacteriol. 1991;173:3789-94 pubmed
    ..A newly constructed insertion allele of exoR has the same phenotype as the original mutant. The deduced sequence of ExoR is 268 amino acids long but does not show homology to other sequenced genes. ..
  2. David M, Daveran M, Batut J, Dedieu A, Domergue O, Ghai J, et al. Cascade regulation of nif gene expression in Rhizobium meliloti. Cell. 1988;54:671-83 pubmed
    ..We propose that FixL, which has features of a transmembrane protein, senses an environmental signal and transduces it to FixJ, a transcriptional activator of nif and fix genes. ..
  3. Osteras M, Stanley J, Finan T. Identification of Rhizobium-specific intergenic mosaic elements within an essential two-component regulatory system of Rhizobium species. J Bacteriol. 1995;177:5485-94 pubmed
    ..meliloti, Rhizobium sp. strain NGR234, Rhizobium leguminosarum, and Agrobacterium rhizogenes, but none was present in A. tumefaciens and Bradyrhizobium japonicum. ..
  4. Willis L, Walker G. The phbC (poly-beta-hydroxybutyrate synthase) gene of Rhizobium (Sinorhizobium) meliloti and characterization of phbC mutants. Can J Microbiol. 1998;44:554-64 pubmed
    ..meliloti phbC gene was determined. The deduced polypeptide sequence is homologous to previously identified PhbCs from other bacteria. The R. meliloti phbC locus maps to pRmeSU47a, the smaller of the two megaplasmids in this strain. ..
  5. Davey M, de Bruijn F. A homologue of the tryptophan-rich sensory protein TspO and FixL regulate a novel nutrient deprivation-induced Sinorhizobium meliloti locus. Appl Environ Microbiol. 2000;66:5353-9 pubmed
    A nutrient deprivation-induced locus in Sinorhizobium meliloti strain 1021 was identified by use of a Tn5-luxAB reporter gene transposon...
  6. Ono Y, Mitsui H, Sato T, Minamisawa K. Two RpoH homologs responsible for the expression of heat shock protein genes in Sinorhizobium meliloti. Mol Gen Genet. 2001;264:902-12 pubmed
    ..meliloti: one requires the rpoH1 function, while rpoH2 can substitute in part for the rpoH1 function. Moreover, the rpoH1 mutant and rpoH1 rpoH2 double mutant exhibited Nod+ Fix- and Nod- phenotypes, respectively, on alfalfa. ..
  7. Lynch D, O Brien J, Welch T, Clarke P, Cuív P, Crosa J, et al. Genetic organization of the region encoding regulation, biosynthesis, and transport of rhizobactin 1021, a siderophore produced by Sinorhizobium meliloti. J Bacteriol. 2001;183:2576-85 pubmed
    ..Mutants having transposon insertions in the biosynthesis or transport genes induced effective nitrogen-fixing nodules on alfalfa plants. ..
  8. Bittner A, Oke V. Multiple groESL operons are not key targets of RpoH1 and RpoH2 in Sinorhizobium meliloti. J Bacteriol. 2006;188:3507-15 pubmed
    ..Taken together, these results suggest that GroEL/GroES production alone cannot explain the requirements for RpoH1 and RpoH2 in S. meliloti and that there must be other crucial targets. ..
  9. Wells D, Chen E, Fisher R, Long S. ExoR is genetically coupled to the ExoS-ChvI two-component system and located in the periplasm of Sinorhizobium meliloti. Mol Microbiol. 2007;64:647-64 pubmed
    ..Our data suggest that periplasmically localized ExoR and ExoS-ChvI function together in a unique and critical regulatory system associated with both free-living and symbiotic states of S. meliloti. ..

More Information

Publications126 found, 100 shown here

  1. Fujishige N, Lum M, De Hoff P, Whitelegge J, Faull K, Hirsch A. Rhizobium common nod genes are required for biofilm formation. Mol Microbiol. 2008;67:504-15 pubmed
  2. Flechard M, Fontenelle C, Trautwetter A, Ermel G, Blanco C. Sinorhizobium meliloti rpoE2 is necessary for H(2)O(2) stress resistance during the stationary growth phase. FEMS Microbiol Lett. 2009;290:25-31 pubmed publisher
    ..A katC strain behaved as an rpoE2 strain during an H(2)O(2) challenge, suggesting that the H(2)O(2) sensitivity of the rpoE2 strain resulted only from the lack of KatC in this strain. ..
  3. Atkinson E, Long S. Homology of Rhizobium meliloti NodC to polysaccharide polymerizing enzymes. Mol Plant Microbe Interact. 1992;5:439-42 pubmed
    ..This similarity is consistent with a role for the NodC protein in the formation of the beta-1,4-linkage in Nod factors. ..
  4. Earl C, Ronson C, Ausubel F. Genetic and structural analysis of the Rhizobium meliloti fixA, fixB, fixC, and fixX genes. J Bacteriol. 1987;169:1127-36 pubmed
  5. Ruvkun G, Sundaresan V, Ausubel F. Directed transposon Tn5 mutagenesis and complementation analysis of Rhizobium meliloti symbiotic nitrogen fixation genes. Cell. 1982;29:551-9 pubmed
    ..meliloti DNA sequences with K. pneumoniae nif gene sequences. R. meliloti nifH, D and K are located in the 6.3 kb fix-::Tn5 cluster and are transcribed in the order nifH, nifD, nifK, which is the same order as in K. pneumoniae. ..
  6. Cheng H, Walker G. Succinoglycan production by Rhizobium meliloti is regulated through the ExoS-ChvI two-component regulatory system. J Bacteriol. 1998;180:20-6 pubmed
    ..We propose a model for regulation of succinoglycan production by R. meliloti through the ExoS-ChvI two-component regulatory system. ..
  7. Kereszt A, Kiss E, Reuhs B, Carlson R, Kondorosi A, Putnoky P. Novel rkp gene clusters of Sinorhizobium meliloti involved in capsular polysaccharide production and invasion of the symbiotic nodule: the rkpK gene encodes a UDP-glucose dehydrogenase. J Bacteriol. 1998;180:5426-31 pubmed
    ..We have demonstrated that RkpK possesses UDP-glucose dehydrogenase activity, while the protein product of ORF1 might function as a UDP-glucuronic acid epimerase. ..
  8. Santos R, Bocquet S, Puppo A, Touati D. Characterization of an atypical superoxide dismutase from Sinorhizobium meliloti. J Bacteriol. 1999;181:4509-16 pubmed
    ..Sequence comparison with 70 FeSODs and MnSODs indicates that S. meliloti SOD contains several atypical residues at specific sites that might account for the activation by manganese and resistance to H(2)O(2) of this unusual Fe-type SOD. ..
  9. Summers M, Botero L, Busse S, McDermott T. The ++Sinorhizobium meliloti lon protease is involved in regulating exopolysaccharide synthesis and is required for nodulation of alfalfa. J Bacteriol. 2000;182:2551-8 pubmed
    ..The results of this study suggest that the S. meliloti Lon protease is important for controlling turnover of a constitutively expressed protein(s) that, when unregulated, disrupts normal nodule formation and normal growth. ..
  10. Oke V, Rushing B, Fisher E, Moghadam Tabrizi M, Long S. Identification of the heat-shock sigma factor RpoH and a second RpoH-like protein in Sinorhizobium meliloti. Microbiology. 2001;147:2399-408 pubmed
    ..An rpoH2-gusA fusion increases in expression during the stationary phase of growth. The presence of two rpoH-like sequences in S. meliloti is reminiscent of the situation in Bradyrhizobium japonicum, which has three rpoH genes. ..
  11. Sauviac L, Philippe H, Phok K, Bruand C. An extracytoplasmic function sigma factor acts as a general stress response regulator in Sinorhizobium meliloti. J Bacteriol. 2007;189:4204-16 pubmed
    ..This therefore suggests that other important actors in the general stress response have still to be identified in S. meliloti. ..
  12. Bahlawane C, McIntosh M, Krol E, Becker A. Sinorhizobium meliloti regulator MucR couples exopolysaccharide synthesis and motility. Mol Plant Microbe Interact. 2008;21:1498-509 pubmed publisher
    ..meliloti Rm2011 was shown to depend on a functional ExpR/Sin quorum-sensing system and the production of both flagella and EPS. Finally, we propose a model for the coordination of motility and EPS synthesis in S. meliloti. ..
  13. Kobayashi H, De Nisco N, Chien P, Simmons L, Walker G. Sinorhizobium meliloti CpdR1 is critical for co-ordinating cell cycle progression and the symbiotic chronic infection. Mol Microbiol. 2009;73:586-600 pubmed publisher
    ..Thus, we demonstrate that CpdR1 mediates the co-ordination of cell cycle events, which are critical for both the free-living cell division and the differentiation required for the chronic intracellular infection. ..
  14. Chen E, Fisher R, Perovich V, Sabio E, Long S. Identification of direct transcriptional target genes of ExoS/ChvI two-component signaling in Sinorhizobium meliloti. J Bacteriol. 2009;191:6833-42 pubmed publisher
    ..Our results provide insight into the mechanism of how ExoS/ChvI regulates its downstream targets and lay a foundation for studying this conserved pathway with critical roles in free-living and symbiotic bacteria. ..
  15. Chen E, Sabio E, Long S. The periplasmic regulator ExoR inhibits ExoS/ChvI two-component signalling in Sinorhizobium meliloti. Mol Microbiol. 2008;69:1290-303 pubmed publisher
    ..Together, our results indicate that ExoR binds to ExoS in the periplasm of S. meliloti to inhibit ExoS/ChvI activity, and that ExoR represents a novel periplasmic inhibitor of two-component signalling...
  16. Queiroux C, Washburn B, Davis O, Stewart J, Brewer T, Lyons M, et al. A comparative genomics screen identifies a Sinorhizobium meliloti 1021 sodM-like gene strongly expressed within host plant nodules. BMC Microbiol. 2012;12:74 pubmed publisher
  17. Koziol U, Hannibal L, Rodr guez M, Fabiano E, Kahn M, Noya F. Deletion of citrate synthase restores growth of Sinorhizobium meliloti 1021 aconitase mutants. J Bacteriol. 2009;191:7581-6 pubmed publisher
    The symbiotic nitrogen-fixing bacterium Sinorhizobium meliloti 1021 encodes only one predicted aconitase (AcnA) in its genome...
  18. Bélanger L, Dimmick K, Fleming J, Charles T. Null mutations in Sinorhizobium meliloti exoS and chvI demonstrate the importance of this two-component regulatory system for symbiosis. Mol Microbiol. 2009;74:1223-37 pubmed publisher
  19. Watson R, Wheatcroft R. Nucleotide sequence of Rhizobium meliloti insertion sequence ISRm1: homology to IS2 from Escherichia coli and IS426 from Agrobacterium tumefaciens. DNA Seq. 1991;2:163-72 pubmed
    ..Comparison of the proteins potentially encoded by these insertion sequences showed that the two ORFs found in ISRm1 are also present in IS2 and IS426, suggesting that they may be functional genes. ..
  20. Schwedock J, Long S. Nucleotide sequence and protein products of two new nodulation genes of Rhizobium meliloti, nodP and nodQ. Mol Plant Microbe Interact. 1989;2:181-94 pubmed
    ..meliloti strain 1021, and in other species of Rhizobium. The nodP gene also displayed homology to E. coli. A computer search revealed significant homology between NodQ and the GDP binding domain of elongation factor Tu (EF-Tu). ..
  21. Leong S, Williams P, Ditta G. Analysis of the 5' regulatory region of the gene for delta-aminolevulinic acid synthetase of Rhizobium meliloti. Nucleic Acids Res. 1985;13:5965-76 pubmed
    ..coli promoter sequence, while the -10 region is dissimilar. No resemblance was found between either P1 or P2 and the promoter regions of genes under general nitrogen control...
  22. Alfano J, Kahn M. Isolation and characterization of a gene coding for a novel aspartate aminotransferase from Rhizobium meliloti. J Bacteriol. 1993;175:4186-96 pubmed
    ..87 mM. Its pH optimum is between 8.0 and 8.5. Mutations were constructed in aatB and tatA and transferred to the genome of R. meliloti 104A14. Both mutants were prototrophs and were able to carry out symbiotic nitrogen fixation...
  23. Turner S, Young J. The glutamine synthetases of rhizobia: phylogenetics and evolutionary implications. Mol Biol Evol. 2000;17:309-19 pubmed publisher
    ..Meta-analysis of both GS genes from the different genera of rhizobia and other reference organisms suggests that the divergence times of the different rhizobium genera predate the existence of legumes, their host plants...
  24. Park S, Meyer M, Jones A, Yennawar H, Yennawar N, Nixon B. Two-component signaling in the AAA + ATPase DctD: binding Mg2+ and BeF3- selects between alternate dimeric states of the receiver domain. FASEB J. 2002;16:1964-6 pubmed publisher
    ..Significant differences exist in the signaling surfaces of the DctD and NtrC receiver domains that may help explain how triggering the common two-component switch can variously regulate assembly of a AAA+ ATPase domain...
  25. Luo M, Gamage T, Arentson B, Schlasner K, Becker D, Tanner J. Structures of Proline Utilization A (PutA) Reveal the Fold and Functions of the Aldehyde Dehydrogenase Superfamily Domain of Unknown Function. J Biol Chem. 2016;291:24065-24075 pubmed
    ..Kinetic data for SmPutA indicate a substrate-channeling mechanism, in agreement with previous studies of other PutAs. ..
  26. Kereszt A, Slaska Kiss K, Putnoky P, Banfalvi Z, Kondorosi A. The cycHJKL genes of Rhizobium meliloti involved in cytochrome c biogenesis are required for "respiratory" nitrate reduction ex planta and for nitrogen fixation during symbiosis. Mol Gen Genet. 1995;247:39-47 pubmed
    ..Moreover, our results indicate that in R. meliloti c-type cytochromes are required for respiratory nitrate reduction ex planta, as well as for symbiotic nitrogen fixation in root nodules. ..
  27. Leach F, Wacks D, Signer E. Rhizobium meliloti homologs of Escherichia coli mur genes. Gene. 1994;148:87-90 pubmed
    ..coli cell wall. The R. meliloti putative murD sequence is preceded by a partial ORF that shares sequence identity with mraY. The orientation of the two ORFs in R. meliloti is similar to that of the E. coli murD and mraY genes. ..
  28. Glazebrook J, Ichige A, Walker G. A Rhizobium meliloti homolog of the Escherichia coli peptide-antibiotic transport protein SbmA is essential for bacteroid development. Genes Dev. 1993;7:1485-97 pubmed
    ..Southern blotting experiments indicate that a gene closely related to bacA/sbmA is found in many bacteria, including some that invade eukaryotic cells. Possible roles for BacA in symbiosis are discussed...
  29. Astete S, Leigh J. mucS, a gene involved in activation of galactoglucan (EPS II) synthesis gene expression in Rhizobium meliloti. Mol Plant Microbe Interact. 1996;9:395-400 pubmed
    ..mucS lies within a cluster of EPS II synthesis genes and contains an open reading frame of 190 amino acids. MucS does not show any significant similarity to known genes and may represent a new type of regulatory protein...
  30. Oster s M, Boncompagni E, Vincent N, Poggi M, Le Rudulier D. Presence of a gene encoding choline sulfatase in Sinorhizobium meliloti bet operon: choline-O-sulfate is metabolized into glycine betaine. Proc Natl Acad Sci U S A. 1998;95:11394-9 pubmed
    ..In conclusion, choline-O-sulfate and phosphorylcholine, which are found in higher plants and fungi, appear to be substrates for glycine betaine biosynthesis in S. meliloti...
  31. Phillips -, Sande -, Vriezen -, de Bruijn FJ -, Le Rudulier D -, Joseph -. A new genetic locus in sinorhizobium meliloti is involved in stachydrine utilization. Appl Environ Microbiol. 1998;64:3954-60 pubmed
    ..All data are consistent with the concept that stcD codes for an enzyme that produces proline by demethylation of N-methylproline, a degradation product of stachydrine. ..
  32. Sigaud S, Becquet V, Frendo P, Puppo A, H rouart D. Differential regulation of two divergent Sinorhizobium meliloti genes for HPII-like catalases during free-living growth and protective role of both catalases during symbiosis. J Bacteriol. 1999;181:2634-9 pubmed
    ..A dramatic decrease of nitrogen fixation capacity in a katA katC double mutant was observed, suggesting that these catalases are very important for the protection of the nitrogen fixation process...
  33. Peck M, Gaal T, Fisher R, Gourse R, Long S. The RNA polymerase alpha subunit from Sinorhizobium meliloti can assemble with RNA polymerase subunits from Escherichia coli and function in basal and activated transcription both in vivo and in vitro. J Bacteriol. 2002;184:3808-14 pubmed
    ..The ability to utilize E. coli as a heterologous system in which to study the regulation of S. meliloti genes could provide an important tool for our understanding and manipulation of these processes...
  34. Wei W, Jiang J, Li X, Wang L, Yang S. Isolation of salt-sensitive mutants from Sinorhizobium meliloti and characterization of genes involved in salt tolerance. Lett Appl Microbiol. 2004;39:278-83 pubmed publisher
    ..Based on these findings, we suggest that the regulation of salt tolerance of S. meliloti 042BM is complex and on several levels...
  35. Keating D. Sinorhizobium meliloti SyrA mediates the transcriptional regulation of genes involved in lipopolysaccharide sulfation and exopolysaccharide biosynthesis. J Bacteriol. 2007;189:2510-20 pubmed
    ..Thus, SyrA likely acts indirectly to promote transcriptional upregulation of lpsS and exo genes through a mechanism that requires the ExoS/ChvI two-component system. ..
  36. Fisher R, Swanson J, Mulligan J, Long S. Extended Region of Nodulation Genes in Rhizobium meliloti 1021. II. Nucleotide Sequence, Transcription Start Sites and Protein Products. Genetics. 1987;117:191-201 pubmed
    ..Its distance from the transcription start site is more suggestive of an activator binding site rather than an RNA polymerase binding site. ..
  37. Davies B, Walker G. A highly conserved protein of unknown function is required by Sinorhizobium meliloti for symbiosis and environmental stress protection. J Bacteriol. 2008;190:1118-23 pubmed publisher
    ..We demonstrate that the SMc01113 gene is absolutely required for S. meliloti symbiosis with alfalfa and also for the protection of the bacterium from a wide range of environmental stresses...
  38. Platt M, Miller K, Lane W, Kennedy E. Isolation and characterization of the constitutive acyl carrier protein from Rhizobium meliloti. J Bacteriol. 1990;172:5440-4 pubmed
    ..coli ACP acylase enzyme. All four ACPs act as acceptors of acyl residues, but only the E. coli ACP functions in the transglucosylase system. ..
  39. Szeto W, Nixon B, Ronson C, Ausubel F. Identification and characterization of the Rhizobium meliloti ntrC gene: R. meliloti has separate regulatory pathways for activation of nitrogen fixation genes in free-living and symbiotic cells. J Bacteriol. 1987;169:1423-32 pubmed
    ..meliloti. These latter results indicate that R. meliloti has separate regulatory pathways for activating nif gene expression ex planta and during symbiotic nitrogen fixation...
  40. Streit W, Phillips D. A biotin-regulated locus, bioS, in a possible survival operon of Rhizobium meliloti. Mol Plant Microbe Interact. 1997;10:933-7 pubmed publisher
    ..The 588-bp bioS is located among three genes showing homology to survival operons of other bacteria, and it may be part of a system that R. meliloti uses to respond to plant biotin signals...
  41. Platzer J, Sterr W, Hausmann M, Schmitt R. Three genes of a motility operon and their role in flagellar rotary speed variation in Rhizobium meliloti. J Bacteriol. 1997;179:6391-9 pubmed
    ..meliloti cell. A working model suggests that interactions between MotB and MotC at the periplasmic surface of the motor control the energy flux or the energy coupling that drives flagellar rotation...
  42. Pelchat M, Gagnon Y, Laberge S, Lapointe J. Co-transcription of Rhizobium meliloti lysyl-tRNA synthetase and glutamyl-tRNA synthetase genes. FEBS Lett. 1999;449:23-7 pubmed
    ..meliloti. This is the first reported case of two immediately adjacent and co-transcribed genes encoding aminoacyl-tRNA synthetases. ..
  43. Martin M, Lloret J, Sanchez Contreras M, Bonilla I, Rivilla R. MucR is necessary for galactoglucan production in Sinorhizobium meliloti EFB1. Mol Plant Microbe Interact. 2000;13:129-35 pubmed
    ..MucR proteins from both strains are interchangeable. An mucR mutant of EFB1 cannot produce galactoglucan and does not express mucS. ..
  44. Fenner B, Tiwari R, Reeve W, Dilworth M, Glenn A. Sinorhizobium medicae genes whose regulation involves the ActS and/or ActR signal transduction proteins. FEMS Microbiol Lett. 2004;236:21-31 pubmed publisher
    ..In particular, we demonstrate that the transcriptional activation of fixN2 is regulated by ActR through FixK...
  45. Sheftic S, White E, Gage D, Alexandrescu A. NMR structure of the HWE kinase associated response regulator Sma0114 in its activated state. Biochemistry. 2014;53:311-22 pubmed publisher
    ..The dynamic character of the 455 face in Sma0114, which results in part from the replacement of helix ?4 by a flexible loop, may facilitate induced-fit recognition of target molecules. ..
  46. Margolin W, Corbo J, Long S. Cloning and characterization of a Rhizobium meliloti homolog of the Escherichia coli cell division gene ftsZ. J Bacteriol. 1991;173:5822-30 pubmed
    ..These results suggest that low levels of FtsZRm stimulate E. coli cell division, while high levels may be inhibitory...
  47. Engelke T, Jording D, Kapp D, P hler A. Identification and sequence analysis of the Rhizobium meliloti dctA gene encoding the C4-dicarboxylate carrier. J Bacteriol. 1989;171:5551-60 pubmed
    ..6 kilodaltons, respectively. The hydrophobicity plot suggests that DctA is a membrane protein with several membrane passages. The amino acid sequences of the R. meliloti and the R. leguminosarum DctA proteins were highly conserved (82%)...
  48. Becker A, Kleickmann A, Keller M, Arnold W, P hler A. Identification and analysis of the Rhizobium meliloti exoAMONP genes involved in exopolysaccharide biosynthesis and mapping of promoters located on the exoHKLAMONP fragment. Mol Gen Genet. 1993;241:367-79 pubmed
    ..A much weaker promoter upstream of exoL was found to be involved in the transcription of the exoLAMONP genes. In addition, weak promoters were identified upstream of exoK, exoA, exoN and exoP...
  49. Bardin S, Dan S, Osteras M, Finan T. A phosphate transport system is required for symbiotic nitrogen fixation by Rhizobium meliloti. J Bacteriol. 1996;178:4540-7 pubmed
    ..Presumably, the PhoCDET transport system is employed by the bacteria in the soil environment, where the concentration of available phosphate is normally 0.1 to 1 microM...
  50. Miyatake H, Kanai M, Adachi S, Nakamura H, Tamura K, Tanida H, et al. Dynamic light-scattering and preliminary crystallographic studies of the sensor domain of the haem-based oxygen sensor FixL from Rhizobium meliloti. Acta Crystallogr D Biol Crystallogr. 1999;55:1215-8 pubmed
    ..94, b = 37.44, c = 54.14 A, beta = 115.29 degrees and diffract X-rays to 1.7 A resolution at station BL44B2 of SPring-8, Japan. Bijvoet difference Patterson maps show a clear peak corresponding to the haem iron in RmFixLH. ..
  51. Miyatake H, Mukai M, Park S, Adachi S, Tamura K, Nakamura H, et al. Sensory mechanism of oxygen sensor FixL from Rhizobium meliloti: crystallographic, mutagenesis and resonance Raman spectroscopic studies. J Mol Biol. 2000;301:415-31 pubmed publisher
    ..In the I209A mutant of RmFixL, the O(2) sensing activity was destroyed, thus confirming our proposed mechanism...
  52. Grzemski W, Akowski J, Kahn M. Probing the Sinorhizobium meliloti-alfalfa symbiosis using temperature-sensitive and impaired-function citrate synthase mutants. Mol Plant Microbe Interact. 2005;18:134-41 pubmed
    ..Microscopic examination of these nodules revealed the loss of bacteroids and senescence, indicating that CS activity was essential for nodule maintenance. ..
  53. Yang H, Cheng J, Finan T, Rosen B, Bhattacharjee H. Novel pathway for arsenic detoxification in the legume symbiont Sinorhizobium meliloti. J Bacteriol. 2005;187:6991-7 pubmed publisher
    ..Thus, AqpS confers arsenate resistance together with ArsC-catalyzed reduction. This is the first report of an aquaglyceroporin with a physiological function in arsenic resistance...
  54. Br nger A, Kuriyan J, Karplus M. Crystallographic R factor refinement by molecular dynamics. Science. 1987;235:458-60 pubmed publisher
    ..In crambin, the dynamics calculation moved residues that were misplaced by more than 3 angstroms into the correct positions without human intervention...
  55. Marlow V, Haag A, Kobayashi H, Fletcher V, Scocchi M, Walker G, et al. Essential role for the BacA protein in the uptake of a truncated eukaryotic peptide in Sinorhizobium meliloti. J Bacteriol. 2009;191:1519-27 pubmed publisher
    ..Further, they provide evidence that the BacA function that leads to the S. meliloti lipid A VLCFA modification plays a key role in the chronic infection of legumes...
  56. Krol E, Becker A. ppGpp in Sinorhizobium meliloti: biosynthesis in response to sudden nutritional downshifts and modulation of the transcriptome. Mol Microbiol. 2011;81:1233-54 pubmed publisher
    ..dksA was required for the majority of the relA-dependent regulations...
  57. Sol s Oviedo R, Mart nez Morales F, Geiger O, Sohlenkamp C. Functional and topological analysis of phosphatidylcholine synthase from Sinorhizobium meliloti. Biochim Biophys Acta. 2012;1821:573-81 pubmed publisher
    ..The majority of the conserved residues is predicted to be either located within the cytoplasmic loops or on the cytoplasmic side of the membrane which can be expected for an enzyme using one membrane-associated and one soluble substrate...
  58. Buendia A, Enenkel B, K plin R, Niehaus K, Arnold W, P hler A. The Rhizobium meliloti exoZl exoB fragment of megaplasmid 2: ExoB functions as a UDP-glucose 4-epimerase and ExoZ shows homology to NodX of Rhizobium leguminosarum biovar viciae strain TOM. Mol Microbiol. 1991;5:1519-30 pubmed
    ..In R. meliloti, exoB codes for a UDP-glucose 4-epimerase. A deficiency in the activity of this enzyme fully accounts for all the multiple carbohydrate defects that have been observed in exoB mutants...
  59. Cervantes E, Sharma S, Maillet F, Vasse J, Truchet G, Rosenberg C. The Rhizobium meliloti host range nodQ gene encodes a protein which shares homology with translation elongation and initiation factors. Mol Microbiol. 1989;3:745-55 pubmed
    ..The predicted amino acid sequence of the NodQ protein shows homology with translation initiation and elongation factors. The consensus sequence involved in the GTP-binding domain is conserved. ..
  60. Laberge S, Gagnon Y, Bordeleau L, Lapointe J. Cloning and sequencing of the gltX gene, encoding the glutamyl-tRNA synthetase of Rhizobium meliloti A2. J Bacteriol. 1989;171:3926-32 pubmed
    ..These results suggest that the C-terminal part of the protein is probably not involved in the recognition of substrates, a feature shared with other aminoacyl-tRNA synthetases. ..
  61. Jiang J, Gu B, Albright L, Nixon B. Conservation between coding and regulatory elements of Rhizobium meliloti and Rhizobium leguminosarum dct genes. J Bacteriol. 1989;171:5244-53 pubmed
  62. Chan Y, McCormick W, Watson R. A new nos gene downstream from nosDFY is essential for dissimilatory reduction of nitrous oxide by Rhizobium (Sinorhizobium) meliloti. Microbiology. 1997;143 ( Pt 8):2817-24 pubmed
    ..It was concluded that there are seven genes constituting the nos cluster in R. meliloti. They are organized in four complementation groups and in the same orientation, spanning a distance of about 9 kb on the nod megaplasmid. ..
  63. Ma X, Sun Q, Wang R, Singh G, Jonietz E, Margolin W. Interactions between heterologous FtsA and FtsZ proteins at the FtsZ ring. J Bacteriol. 1997;179:6788-97 pubmed
    ..coli proteins and those of the two other species may be important for specific interactions. ..
  64. Charles T, Aneja P. Methylmalonyl-CoA mutase encoding gene of Sinorhizobium meliloti. Gene. 1999;226:121-7 pubmed
    ..Downstream of, and oriented towards bhbA, was identified a member of the GNTR class of transcriptional regulator-encoding genes. It is not yet known whether this regulatory protein is directly involved in modulation of bhbA expression...
  65. Voegele R, Mitsch M, Finan T. Characterization of two members of a novel malic enzyme class. Biochim Biophys Acta. 1999;1432:275-85 pubmed
    ..Our characterization of the two R. meliloti malic enzymes therefore suggests a number of features uncharacteristic for malic enzymes described so far. ..
  66. Soto M, Jim nez Zurdo J, van Dillewijn P, Toro N. Sinorhizobium meliloti putA gene regulation: a new model within the family Rhizobiaceae. J Bacteriol. 2000;182:1935-41 pubmed
    ..However, some differences have been found with the latter model: (i) S. meliloti putA gene is not catabolite repressed, and (ii) the gene encoding for the major proline permease (putP) does not form part of an operon with the putA gene...
  67. Mart nez Abarca F, Garc a Rodr guez F, Toro N. Homing of a bacterial group II intron with an intron-encoded protein lacking a recognizable endonuclease domain. Mol Microbiol. 2000;35:1405-12 pubmed
    ..Afterwards, the remaining intronless target DNA is protected from intron invasion...
  68. Lagares A, Hozbor D, Niehaus K, Otero A, Lorenzen J, Arnold W, et al. Genetic characterization of a Sinorhizobium meliloti chromosomal region in lipopolysaccharide biosynthesis. J Bacteriol. 2001;183:1248-58 pubmed publisher
    ..meliloti lpsB also encodes a mannosyltransferase that participates in the biosynthesis of the LPS core. Evidence is provided for the presence of other lpsB-homologous sequences in several members of the family Rhizobiaceae...
  69. Sohlenkamp C, de Rudder K, Geiger O. Phosphatidylethanolamine is not essential for growth of Sinorhizobium meliloti on complex culture media. J Bacteriol. 2004;186:1667-77 pubmed
    ..Therefore, although choline permits Pcs-dependent PC formation in the mutant, it does not restore wild-type-like growth in minimal medium, suggesting that it is not only the lack of PC that leads to this drastic growth phenotype. ..
  70. Mart nez Rodr guez S, And jar S nchez M, Neira J, Clemente Jim nez J, Jara P rez V, Rodr guez Vico F, et al. Site-directed mutagenesis indicates an important role of cysteines 76 and 181 in the catalysis of hydantoin racemase from Sinorhizobium meliloti. Protein Sci. 2006;15:2729-38 pubmed publisher
    ..This recognition process is further supported by measurements of protein stability followed by chemical denaturation in the presence of the corresponding compound...
  71. Santos M, Cosme A, Becker J, Medeiros J, Mata M, Moreira L. Absence of functional TolC protein causes increased stress response gene expression in Sinorhizobium meliloti. BMC Microbiol. 2010;10:180 pubmed publisher
    ..This finding further underlines the fundamental role of this protein in Sinorhizobium meliloti biology...
  72. Lipuma J, Cinege G, Bodogai M, Oláh B, Kiers A, Endre G, et al. A vapBC-type toxin-antitoxin module of Sinorhizobium meliloti influences symbiotic efficiency and nodule senescence of Medicago sativa. Environ Microbiol. 2014;16:3714-29 pubmed publisher
    ..These data indicate that modification of the toxin/antitoxin production may influence bacteroid metabolism and may have an impact on the adaptation to changing environmental conditions. ..
  73. de Lucena D, P hler A, Weidner S. The role of sigma factor RpoH1 in the pH stress response of Sinorhizobium meliloti. BMC Microbiol. 2010;10:265 pubmed publisher
    ..This study provided clear evidence that the sigma factor RpoH1 plays a major role in pH stress response...
  74. Honma M, Asomaning M, Ausubel F. Rhizobium meliloti nodD genes mediate host-specific activation of nodABC. J Bacteriol. 1990;172:901-11 pubmed
    ..meliloti NodD1 and NodD2 are highly homologous except in the C-terminal region. Our results support the hypothesis that R. meliloti utilizes the three copies of nodD to optimize the interaction with each of its legume hosts...
  75. Tombolini R, Povolo S, Buson A, Squartini A, Nuti M. Poly-beta-hydroxybutyrate (PHB) biosynthetic genes in Rhizobium meliloti 41. Microbiology. 1995;141 ( Pt 10):2553-9 pubmed publisher
    ..meliloti 41. The three genes were sufficient to direct the production of polyhydroxyalkanoate in E. coli. The homology of ORF1 with an ORF located near the PHB genes in two phototrophic bacteria suggests its involvement in PHB synthesis...
  76. Holloway P, McCormick W, Watson R, Chan Y. Identification and analysis of the dissimilatory nitrous oxide reduction genes, nosRZDFY, of Rhizobium meliloti. J Bacteriol. 1996;178:1505-14 pubmed
    ..stutzeri was absent in R. meliloti. No rpoN-binding site preceding the nos genes was detected, and none of the Tn5 insertions in the nos gene region affected symbiotic N2-fixing ability...
  77. Jim nez Zurdo J, Garc a Rodr guez F, Toro N. The Rhizobium meliloti putA gene: its role in the establishment of the symbiotic interaction with alfalfa. Mol Microbiol. 1997;23:85-93 pubmed
    ..Furthermore, we show that the PutA- phenotype leads to a significant reduction of alfalfa root colonization by R. meliloti...
  78. Aneja P, Charles T. Poly-3-hydroxybutyrate degradation in Rhizobium (Sinorhizobium) meliloti: isolation and characterization of a gene encoding 3-hydroxybutyrate dehydrogenase. J Bacteriol. 1999;181:849-57 pubmed
    ..Studies with a strain carrying a lacZ transcriptional fusion to bdhA demonstrated that gene expression is growth phase associated...
  79. Tapias A, Barb J. Regulation of divergent transcription from the uvrA-ssb promoters in Sinorhizobium meliloti. Mol Gen Genet. 1999;262:121-30 pubmed
    ..Comparison of the sequences of recA and uvrA promoters from different bacterial species of the alpha group of the Proteobacteria has identified the direct repeat GTTCYYKTTTTGTTC as the SOS box in this phylogenetic group...
  80. Snoeck C, Verreth C, Hern ndez Lucas I, Mart nez Romero E, Vanderleyden J. Identification of a third sulfate activation system in Sinorhizobium sp. strain BR816: the CysDN sulfate activation complex. Appl Environ Microbiol. 2003;69:2006-14 pubmed
  81. Banci L, Bertini I, Cantini F, Ciofi Baffoni S, Gonnelli L, Mangani S. Solution structure of Cox11, a novel type of beta-immunoglobulin-like fold involved in CuB site formation of cytochrome c oxidase. J Biol Chem. 2004;279:34833-9 pubmed publisher
    ..The present results advance the knowledge on the poorly understood molecular aspects of cytochrome c oxidase assembly...
  82. Vinardell J, Ollero F, Hidalgo A, L pez Baena F, Medina C, Ivanov Vangelov K, et al. NolR regulates diverse symbiotic signals of Sinorhizobium fredii HH103. Mol Plant Microbe Interact. 2004;17:676-85 pubmed publisher
    ..The nolR gene was positively identified in all S. fredii strains investigated, S. xinjiangense CCBAU110, and S. saheli USDA4102. Apparently, S. teranga USDA4101 does not contain this gene...
  83. Dymov S, Meek D, Steven B, Driscoll B. Insertion of transposon Tn5tac1 in the Sinorhizobium meliloti malate dehydrogenase (mdh) gene results in conditional polar effects on downstream TCA cycle genes. Mol Plant Microbe Interact. 2004;17:1318-27 pubmed publisher
    ..Cosmid clone pDS15 restored MDH activity to Rm30049, complemented both the mutant growth and symbiotic phenotypes, and was found to carry six complete (sdhB, mdh, sucCDAB) and two partial (IpdA, sdhA) tricarboxylic acid cycle genes...
  84. Jamet A, Kiss E, Batut J, Puppo A, H rouart D. The katA catalase gene is regulated by OxyR in both free-living and symbiotic Sinorhizobium meliloti. J Bacteriol. 2005;187:376-81 pubmed publisher
    ..meliloti. Moreover, oxyR is expressed independently of exogenous H2O2 and downregulates its own expression in S. meliloti...
  85. Cheng J, Sibley C, Zaheer R, Finan T. A Sinorhizobium meliloti minE mutant has an altered morphology and exhibits defects in legume symbiosis. Microbiology. 2007;153:375-87 pubmed publisher
    ..coli and S. meliloti min genes. These data suggest that there is greater redundancy in the roles of cell division genes in S. meliloti compared with E. coli...
  86. Yurgel S, Kahn M. A mutant GlnD nitrogen sensor protein leads to a nitrogen-fixing but ineffective Sinorhizobium meliloti symbiosis with alfalfa. Proc Natl Acad Sci U S A. 2008;105:18958-63 pubmed publisher
    ..These results indicate that bacterial nitrogen stress regulation is important to symbiotic productivity and suggest that GlnD may act in a novel way to influence symbiotic behavior...
  87. Bastiat B, Sauviac L, Bruand C. Dual control of Sinorhizobium meliloti RpoE2 sigma factor activity by two PhyR-type two-component response regulators. J Bacteriol. 2010;192:2255-65 pubmed publisher
    ..meliloti adds an unexpected level of complexity, which may allow the regulatory system to integrate multiple stimuli...
  88. Wehmeier S, Arnold M, Marlow V, Aouida M, Myka K, Fletcher V, et al. Internalization of a thiazole-modified peptide in Sinorhizobium meliloti occurs by BacA-dependent and -independent mechanisms. Microbiology. 2010;156:2702-13 pubmed publisher
    ..meliloti. We determined that the BacA-dependent and -independent mechanisms of bleomycin uptake are energy-dependent, consistent with both mechanisms of bleomycin uptake involving transport systems...
  89. Reed J, Walker G. Acidic conditions permit effective nodulation of alfalfa by invasion-deficient Rhizobium meliloti exoD mutants. Genes Dev. 1991;5:2274-87 pubmed
    ..We propose models whereby absence of a putative membrane protein might lead to sensitivity to alkaline conditions and consequent arrest of nodule invasion...
  90. Kahn D, Ditta G. Modular structure of FixJ: homology of the transcriptional activator domain with the -35 binding domain of sigma factors. Mol Microbiol. 1991;5:987-97 pubmed
    ..Oligonucleotide-directed mutagenesis of the transcriptional activator module demonstrated the importance of a potential helix-turn-helix structure...
  91. Reed J, Capage M, Walker G. Rhizobium meliloti exoG and exoJ mutations affect the exoX-exoY system for modulation of exopolysaccharide production. J Bacteriol. 1991;173:3776-88 pubmed
    ..The deduced sequence of ExoY is homologous to a protein required for an early step in xanthan gum biosynthesis, further suggesting that the modulatory system may affect the exopolysaccharide biosynthetic apparatus...
  92. Klipp W, Reil nder H, Schl ter A, Krey R, P hler A. The Rhizobium meliloti fdxN gene encoding a ferredoxin-like protein is necessary for nitrogen fixation and is cotranscribed with nifA and nifB. Mol Gen Genet. 1989;216:293-302 pubmed
    ..This low-level activation of the nifB promoter may be the reason why transcription of nifB and fdxN is initiated primarily at a promoter in front of nifA...