Rhodobacter sphaeroides 2.4.1


Alias: Rhodobacter sphaeroides ATCC 17023, Rhodobacter sphaeroides ATH 2.4.1, Rhodobacter sphaeroides str. 2.4.1, Rhodobacter sphaeroides strain 2.4.1

Top Publications

  1. Gomelsky M, Kaplan S. Isolation of regulatory mutants in photosynthesis gene expression in Rhodobacter sphaeroides 2.4.1 and partial complementation of a PrrB mutant by the HupT histidine-kinase. Microbiology. 1995;141 ( Pt 8):1805-19 pubmed
    ..We also report the cloning, organization and structure of some of the hup genes from R. sphaeroides and construction of a Hup- strain. ..
  2. Eraso J, Roh J, Zeng X, Callister S, Lipton M, Kaplan S. Role of the global transcriptional regulator PrrA in Rhodobacter sphaeroides 2.4.1: combined transcriptome and proteome analysis. J Bacteriol. 2008;190:4831-48 pubmed publisher
    ..lacZ transcriptional and kan translational fusions enabled us to map putative PrrA binding sites and revealed potential gene targets for indirect regulation by PrrA. ..
  3. Peuser V, Glaeser J, Klug G. The RSP_2889 gene product of Rhodobacter sphaeroides is a CueR homologue controlling copper-responsive genes. Microbiology. 2011;157:3306-13 pubmed publisher
    ..While deletion of RSP_2889 has no significant effect on copper resistance, expression from a low-copy-number plasmid mediates increased sensitivity to copper. ..
  4. Zeilstra Ryalls J, Kaplan S. Aerobic and anaerobic regulation in Rhodobacter sphaeroides 2.4.1: the role of the fnrL gene. J Bacteriol. 1995;177:6422-31 pubmed
    ..Given the lack of hemT expression under these conditions, we consider FnrL regulation of hemA expression to be a major factor in bringing about changes in the level of ALA synthase activity in response to changes in oxygen tension. ..
  5. Eraso J, Kaplan S. Oxygen-insensitive synthesis of the photosynthetic membranes of Rhodobacter sphaeroides: a mutant histidine kinase. J Bacteriol. 1995;177:2695-706 pubmed
    ..The genetic organization of the photosynthesis response regulatory (PRR) region is discussed. ..
  6. Newman J, Falkowski M, Schilke B, Anthony L, Donohue T. The Rhodobacter sphaeroides ECF sigma factor, sigma(E), and the target promoters cycA P3 and rpoE P1. J Mol Biol. 1999;294:307-20 pubmed
    ..Based on our observations, we present a model in which cohemin resistance is conferred by increasing sigma(E) activity. ..
  7. Anthony J, Warczak K, Donohue T. A transcriptional response to singlet oxygen, a toxic byproduct of photosynthesis. Proc Natl Acad Sci U S A. 2005;102:6502-7 pubmed
    ..Gene products encoded by four newly identified sigma(E)-dependent operons are predicted to be involved in stress response, protecting cells from 1O2 damage, or the conservation of energy. ..
  8. Han Y, Meyer M, Keusgen M, Klug G. A haem cofactor is required for redox and light signalling by the AppA protein of Rhodobacter sphaeroides. Mol Microbiol. 2007;64:1090-104 pubmed
    ..Based on this we present a model for the transmission of light and redox signals by AppA. ..
  9. Howard E, Sun S, Biers E, Moran M. Abundant and diverse bacteria involved in DMSP degradation in marine surface waters. Environ Microbiol. 2008;10:2397-410 pubmed publisher

More Information

Publications107 found, 100 shown here

  1. Sullivan M, Curson A, Shearer N, Todd J, Green R, Johnston A. Unusual regulation of a leaderless operon involved in the catabolism of dimethylsulfoniopropionate in Rhodobacter sphaeroides. PLoS ONE. 2011;6:e15972 pubmed publisher
    ..sphaeroides acuR-acuI-dddL. ..
  2. Zeller T, Mraheil M, Moskvin O, Li K, Gomelsky M, Klug G. Regulation of hydrogen peroxide-dependent gene expression in Rhodobacter sphaeroides: regulatory functions of OxyR. J Bacteriol. 2007;189:3784-92 pubmed
    ..Our data reveal that the OxyR-mediated response is fast and transient. In addition, we found that additional regulatory pathways are involved in the H(2)O(2) response. ..
  3. Ranson Olson B, Zeilstra Ryalls J. Regulation of the Rhodobacter sphaeroides 2.4.1 hemA gene by PrrA and FnrL. J Bacteriol. 2008;190:6769-78 pubmed publisher
    ..We present evidence suggesting that the indirect action of FnrL works through PrrA and discuss possible mechanisms. ..
  4. Schilke B, Donohue T. ChrR positively regulates transcription of the Rhodobacter sphaeroides cytochrome c2 gene. J Bacteriol. 1995;177:1929-37 pubmed
    ..Instead, our results indicated that ChrR and the response regulator PrrA (J. M. Eraso and S. Kaplan, J. Bacteriol. 176:32-43, 1994) functioned independently at the upstream cycA promoter that is activated under anaerobic conditions. ..
  5. Eraso J, Kaplan S. prrA, a putative response regulator involved in oxygen regulation of photosynthesis gene expression in Rhodobacter sphaeroides. J Bacteriol. 1994;176:32-43 pubmed
    ..Kaplan, J. Bacteriol. 174:1146-1157, 1992) of the puc operon regulatory region. Finally, two spontaneous prrA mutations that abolish prrA function by changing amino acids in the amino-terminal domain of the protein were isolated. ..
  6. Campbell E, Greenwell R, Anthony J, Wang S, Lim L, Das K, et al. A conserved structural module regulates transcriptional responses to diverse stress signals in bacteria. Mol Cell. 2007;27:793-805 pubmed
    ..These ASDs are fused to diverse C-terminal domains that are likely involved in responding to specific environmental signals that control the activity of their cognate sigma factor. ..
  7. Ratcliffe E, Tunnicliffe R, Ng I, Adams P, Qian P, Holden Dye K, et al. Experimental evidence that the membrane-spanning helix of PufX adopts a bent conformation that facilitates dimerisation of the Rhodobacter sphaeroides RC-LH1 complex through N-terminal interactions. Biochim Biophys Acta. 2011;1807:95-107 pubmed publisher
  8. Gomelsky M, Kaplan S. appA, a novel gene encoding a trans-acting factor involved in the regulation of photosynthesis gene expression in Rhodobacter sphaeroides 2.4.1. J Bacteriol. 1995;177:4609-18 pubmed
    ..The appA gene was sequenced and appears to encode a protein of 450 amino acids with no obvious homology to known proteins...
  9. Sabaty M, Kaplan S. mgpS, a complex regulatory locus involved in the transcriptional control of the puc and puf operons in Rhodobacter sphaeroides 2.4.1. J Bacteriol. 1996;178:35-45 pubmed
    ..A smaller protein containing the 472 C-terminal amino acids of MgpS is able to act by itself as an activator of puc transcription and is expressed independently of the large open reading frame in which it is contained...
  10. Gomelsky M, Kaplan S. The Rhodobacter sphaeroides 2.4.1 rho gene: expression and genetic analysis of structure and function. J Bacteriol. 1996;178:1946-54 pubmed
    ..The gene downstream of rho is homologous to thdF, whose product is involved in thiophene and furan oxidation...
  11. Oh J, Kaplan S. The cbb3 terminal oxidase of Rhodobacter sphaeroides 2.4.1: structural and functional implications for the regulation of spectral complex formation. Biochemistry. 1999;38:2688-96 pubmed publisher
  12. MacKenzie C, Simmons A, Kaplan S. Multiple chromosomes in bacteria. The yin and yang of trp gene localization in Rhodobacter sphaeroides 2.4.1. Genetics. 1999;153:525-38 pubmed
    ..When taken with the finding that CII encodes essential housekeeping functions, the overall impression is one of close regulatory and functional integration between these chromosomes...
  13. Roh J, Kaplan S. Genetic and phenotypic analyses of the rdx locus of Rhodobacter sphaeroides 2.4.1. J Bacteriol. 2000;182:3475-81 pubmed
    ..This phenotype is also suggested to be the result of the role of the rdxBHIS locus in cbb(3) oxidase activity and/or structure. RdxI is suggested to be a new class of metal transporter of the CPx-type ATPases...
  14. Mouncey N, Gak E, Choudhary M, Oh J, Kaplan S. Respiratory pathways of Rhodobacter sphaeroides 2.4.1(T): identification and characterization of genes encoding quinol oxidases. FEMS Microbiol Lett. 2000;192:205-10 pubmed
    ..No role was found for the Qox oxidase, nor was a qoxB::lacZ transcriptional fusion expressed under a variety of conditions. These are the first molecular studies to characterize the quinol oxidases of R. sphaeroides 2.4.1(T)...
  15. Oh J, Kaplan S. Oxygen adaptation. The role of the CcoQ subunit of the cbb3 cytochrome c oxidase of Rhodobacter sphaeroides 2.4.1. J Biol Chem. 2002;277:16220-8 pubmed publisher
  16. Happ H, Braatsch S, Broschek V, Osterloh L, Klug G. Light-dependent regulation of photosynthesis genes in Rhodobacter sphaeroides 2.4.1 is coordinately controlled by photosynthetic electron transport via the PrrBA two-component system and the photoreceptor AppA. Mol Microbiol. 2005;58:903-14 pubmed publisher
    ..Both signalling pathways involve redox-dependent steps that finally determine the effect of light on gene expression...
  17. Ranson Olson B, Jones D, Donohue T, Zeilstra Ryalls J. In vitro and in vivo analysis of the role of PrrA in Rhodobacter sphaeroides 2.4.1 hemA gene expression. J Bacteriol. 2006;188:3208-18 pubmed publisher
    ..In the case of the hemA gene, we discuss possibilities as to how these new insights can be accommodated within the context of what has already been established for hemA transcription regulation in R. sphaeroides...
  18. Kim Y, Ko I, Lee J, Kang H, Kim Y, Kaplan S, et al. Dominant role of the cbb3 oxidase in regulation of photosynthesis gene expression through the PrrBA system in Rhodobacter sphaeroides 2.4.1. J Bacteriol. 2007;189:5617-25 pubmed publisher
    ..Instead, it is probable that signaling through H303 of the CcoN subunit of the cbb(3) oxidase is part of the pathway through which the cbb(3) oxidase affects the relative kinase/phosphatase activity of the membrane-bound PrrB...
  19. Gomelsky L, Moskvin O, Stenzel R, Jones D, Donohue T, Gomelsky M. Hierarchical regulation of photosynthesis gene expression by the oxygen-responsive PrrBA and AppA-PpsR systems of Rhodobacter sphaeroides. J Bacteriol. 2008;190:8106-14 pubmed publisher
  20. Nuss A, Glaeser J, Klug G. RpoH(II) activates oxidative-stress defense systems and is controlled by RpoE in the singlet oxygen-dependent response in Rhodobacter sphaeroides. J Bacteriol. 2009;191:220-30 pubmed publisher
    ..Therefore, we conclude that systems pivotal for the organism's defense against photooxidative stress are strongly dependent on GSH and are specifically recognized by RpoH(II) in R. sphaeroides...
  21. Eraso J, Kaplan S. Half-Site DNA sequence and spacing length contributions to PrrA binding to PrrA site 2 of RSP3361 in Rhodobacter sphaeroides 2.4.1. J Bacteriol. 2009;191:4353-64 pubmed publisher
    ..We demonstrate that PrrA can bind in vitro to DNA sequences with different lengths in the spacer regions between the half-sites...
  22. Greenwell R, Nam T, Donohue T. Features of Rhodobacter sphaeroides ChrR required for stimuli to promote the dissociation of ?(E)/ChrR complexes. J Mol Biol. 2011;407:477-91 pubmed publisher
    ..Finally, we found that the same aspects of R. sphaeroides ChrR needed for a response to (1)O(2) are required for the dissociation of ?(E)/ChrR complexes in the presence of the organic hydroperoxide t-butyl hydroperoxide...
  23. Yin L, Dragnea V, Bauer C. PpsR, a regulator of heme and bacteriochlorophyll biosynthesis, is a heme-sensing protein. J Biol Chem. 2012;287:13850-8 pubmed publisher
    ..Thus, PpsR functions as both a redox and heme sensor to coordinate the amount of heme, bacteriochlorophyll, and photosystem apoprotein synthesis thereby providing fine tune control to avoid excess free tetrapyrrole accumulation...
  24. Gomelsky M, Kaplan S. Genetic evidence that PpsR from Rhodobacter sphaeroides 2.4.1 functions as a repressor of puc and bchF expression. J Bacteriol. 1995;177:1634-7 pubmed
    ..The carboxy terminus of PpsR, containing the putative DNA-binding domain, by itself possesses repressor activity. Intact palindromes having the motif TGT-N12-ACA are required for PpsR activity...
  25. Choudhary M, Kaplan S. DNA sequence analysis of the photosynthesis region of Rhodobacter sphaeroides 2.4.1. Nucleic Acids Res. 2000;28:862-7 pubmed
    ..The difference in gene organization relative to pucBAC and cycA suggests that this region originated independently of the photosynthesis gene cluster of R.sphaeroides...
  26. Sarma R, Barney B, Hamilton T, Jones A, Seefeldt L, Peters J. Crystal structure of the L protein of Rhodobacter sphaeroides light-independent protochlorophyllide reductase with MgADP bound: a homologue of the nitrogenase Fe protein. Biochemistry. 2008;47:13004-15 pubmed publisher
  27. Eraso J, Kaplan S. Regulation of gene expression by PrrA in Rhodobacter sphaeroides 2.4.1: role of polyamines and DNA topology. J Bacteriol. 2009;191:4341-52 pubmed publisher
    ..We propose that polyamines and DNA supercoiling act synergistically to regulate expression of the RSP3361 gene, partly by affecting the affinity of PrrA binding to the PrrA site 2 within the RSP3361 gene...
  28. Erb T, Frerichs Revermann L, Fuchs G, Alber B. The apparent malate synthase activity of Rhodobacter sphaeroides is due to two paralogous enzymes, (3S)-Malyl-coenzyme A (CoA)/{beta}-methylmalyl-CoA lyase and (3S)- Malyl-CoA thioesterase. J Bacteriol. 2010;192:1249-58 pubmed publisher
    ..Mcl1 and Mcl2 are both related to malate synthase, an enzyme which catalyzes both a Claisen condensation and thioester hydrolysis reaction...
  29. Schneider K, Asao M, Carter M, Alber B. Rhodobacter sphaeroides uses a reductive route via propionyl coenzyme A to assimilate 3-hydroxypropionate. J Bacteriol. 2012;194:225-32 pubmed publisher
  30. Stein M, Schafer A, Giffhorn F. Cloning, nucleotide sequence, and overexpression of smoS, a component of a novel operon encoding an ABC transporter and polyol dehydrogenases of Rhodobacter sphaeroides Si4. J Bacteriol. 1997;179:6335-40 pubmed
    ..SDH was purified to homogeneity, with a recovery of 49%, on the basis of a three-step procedure. Upstream from smoS, another gene (smoK), which encoded a putative ATP-binding protein of an ABC transporter, was identified...
  31. Zarzycki J, Schlichting A, Strychalsky N, M ller M, Alber B, Fuchs G. Mesaconyl-coenzyme A hydratase, a new enzyme of two central carbon metabolic pathways in bacteria. J Bacteriol. 2008;190:1366-74 pubmed publisher
    ..A similar yet distinct class of enzymes containing only one hydratase domain was found in various other bacteria, such as Streptomyces species. The role of this widely distributed new enzyme is discussed...
  32. Yamamoto I, Wada N, Ujiiye T, Tachibana M, Matsuzaki M, Kajiwara H, et al. Cloning and nucleotide sequence of the gene encoding dimethyl sulfoxide reductase from Rhodobacter sphaeroides f. sp. denitrificans. Biosci Biotechnol Biochem. 1995;59:1850-5 pubmed
    ..The deduced amino acid sequence had high homology with those of some enzymes containing a molybdenum cofactor: trimethyl amine N-oxide reductase (48%), biotin sulfoxide reductase (44%), and DMSO reductase (29%) of Escherichia coli. ..
  33. Mizoguchi H, Masuda T, Nishimura K, Shimada H, Ohta H, Shioi Y, et al. Nucleotide sequence and transcriptional analysis of the flanking region of the gene (spb) for the trans-acting factor that controls light-mediated expression of the puf operon in Rhodobacter sphaeroides. Plant Cell Physiol. 1997;38:558-67 pubmed
    ..coli, which are glycosyl transferases involved in the synthesis of lipopolysaccharide. orf318 was transcribed in the opposite direction to ahcY, and at only a low level, under all conditions tested. ..
  34. Makihara F, Tsuzuki M, Sato K, Masuda S, Nagashima K, Abo M, et al. Role of trehalose synthesis pathways in salt tolerance mechanism of Rhodobacter sphaeroides f. sp. denitrificans IL106. Arch Microbiol. 2005;184:56-65 pubmed publisher
    ..This is the first report of an organism using multiple pathways to synthesize trehalose solely for use as a compatible solute against salt stress...
  35. Mueller Cajar O, Stotz M, Wendler P, Hartl F, Bracher A, Hayer Hartl M. Structure and function of the AAA+ protein CbbX, a red-type Rubisco activase. Nature. 2011;479:194-9 pubmed publisher
    ..Understanding Rubisco activation may facilitate efforts to improve CO(2) uptake and biomass production by photosynthetic organisms...
  36. Jones M, Visschers R, van Grondelle R, Hunter C. Construction and characterization of a mutant of Rhodobacter sphaeroides with the reaction center as the sole pigment-protein complex. Biochemistry. 1992;31:4458-65 pubmed
    ..A second strain, named RCLH11, which is devoid of the peripheral LH2 antenna complex has also been constructed. A description of the properties of these strains is presented...
  37. Gibson J, Chen J, Tower P, Tabita F. The form II fructose 1,6-bisphosphatase and phosphoribulokinase genes form part of a large operon in Rhodobacter sphaeroides: primary structure and insertional mutagenesis analysis. Biochemistry. 1990;29:8085-93 pubmed
    ..sphaeroides enzymes, including a protease-sensitive area located in a region equivalent to residues 51-71 of mammalian FBPase.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  38. Williams J, Steiner L, Feher G, Simon M. Primary structure of the L subunit of the reaction center from Rhodopseudomonas sphaeroides. Proc Natl Acad Sci U S A. 1984;81:7303-7 pubmed
    ..The L subunit was found to be composed of 281 amino acids (Mr 31,319) and to contain five hydrophobic segments. It is homologous to the M subunit and to a plant thylakoid protein referred to as the QB or Mr 32,000 protein...
  39. Bartnikas T, Tosques I, Laratta W, Shi J, Shapleigh J. Characterization of the nitric oxide reductase-encoding region in Rhodobacter sphaeroides 2.4.3. J Bacteriol. 1997;179:3534-40 pubmed
    ..Regulatory studies demonstrated that the first four genes, norCBQD, are expressed only when the oxygen concentration is low and nitrate is present but that the two distal genes, nnrTU, are expressed constitutively...
  40. Garc a N, Campos A, Osorio A, Poggio S, Gonz lez Pedrajo B, Camarena L, et al. The flagellar switch genes fliM and fliN of Rhodobacter sphaeroides are contained in a large flagellar gene cluster. J Bacteriol. 1998;180:3978-82 pubmed
    ..Complementation analysis of this fliM mutant indicated that fliM and fliN transcription starts beyond the 5' end of fliK and terminates after fliN...
  41. Axelrod H, Abresch E, Paddock M, Okamura M, Feher G. Determination of the binding sites of the proton transfer inhibitors Cd2+ and Zn2+ in bacterial reaction centers. Proc Natl Acad Sci U S A. 2000;97:1542-7 pubmed
    ..The position of the Cd(2+) and Zn(2+) localizes the proton entry into the RC near Asp-H124, His-H126, and His-H128. Based on the location of the metal, likely pathways of proton transfer from the aqueous surface to Q(B) are proposed. ..
  42. Klug R, Benning C. Two enzymes of diacylglyceryl-O-4'-(N,N,N,-trimethyl)homoserine biosynthesis are encoded by btaA and btaB in the purple bacterium Rhodobacter sphaeroides. Proc Natl Acad Sci U S A. 2001;98:5910-5 pubmed publisher
    ..Because the equivalent reactions have been proposed for different eukaryotes, it seems likely that orthologs of btaA/btaB may be present in other betaine lipid-containing organisms...
  43. Axelrod H, Feher G, Allen J, Chirino A, Day M, Hsu B, et al. Crystallization and X-ray structure determination of cytochrome c2 from Rhodobacter sphaeroides in three crystal forms. Acta Crystallogr D Biol Crystallogr. 1994;50:596-602 pubmed publisher
    ..This binding displaces the sulfur atom of Met l00, which forms the axial ligand in the triclinic and tetragonal structures...
  44. Ferguson Miller S, Hiser C, Liu J. Gating and regulation of the cytochrome c oxidase proton pump. Biochim Biophys Acta. 2012;1817:489-94 pubmed publisher
    ..Mechanistic interpretation of these findings and their significance for understanding energy regulation is discussed. ..
  45. MacGregor B, Donohue T. Evidence for two promoters for the cytochrome c2 gene (cycA) of Rhodobacter sphaeroides. J Bacteriol. 1991;173:3949-57 pubmed
    ..DNA sequences with homology to the Escherichia coli sigma 70 consensus promoter sequence precede the 5' ends at -28 and -274, and there is weak homology upstream of the -82 and -250 ends. ..
  46. Purvis D, Theiler R, Niederman R. Chromatographic and protein chemical analysis of the ubiquinol-cytochrome c2 oxidoreductase isolated from Rhodobacter sphaeroides. J Biol Chem. 1990;265:1208-15 pubmed
    ..Tryptic fragments were also used to generate sequence information for cytochrome c1. ..
  47. Allen J, Feher G, Yeates T, Rees D, Deisenhofer J, Michel H, et al. Structural homology of reaction centers from Rhodopseudomonas sphaeroides and Rhodopseudomonas viridis as determined by x-ray diffraction. Proc Natl Acad Sci U S A. 1986;83:8589-93 pubmed
    ..Unlike the RCs from R. viridis, both quinones are retained in the RCs from R. sphaeroides. The secondary quinone is located near the position related by the 2-fold symmetry axis to the primary quinone...
  48. Xu X, Kadokura H, Okubo A, Kitamoto K, Yamazaki S. Cloning and sequencing of a gene encoding a novel salt stress-induced membrane protein from Rhodobacter sphaeroides f. sp. dentrificans. Appl Microbiol Biotechnol. 2001;56:442-7 pubmed
    ..The protein was present in the outer membrane as a transmembrane protein and was specifically induced by salt stress, but not by heat shock. ..
  49. Hartsock A, Shapleigh J. Mechanisms of oxygen inhibition of nirK expression in Rhodobacter sphaeroides. Microbiology. 2010;156:3158-65 pubmed publisher
    ..The protein probably being affected by these O(2) levels is the transcriptional regulator NnrR. ..
  50. Nowak C, Laredo T, Gebert J, Lipkowski J, Gennis R, Ferguson Miller S, et al. 2D-SEIRA spectroscopy to highlight conformational changes of the cytochrome c oxidase induced by direct electron transfer. Metallomics. 2011;3:619-27 pubmed publisher
    ..The activated state was characterized by a higher number of correlated transitions as well as a higher number of amino acids associated with electron transfer...
  51. Yeates T, Komiya H, Rees D, Allen J, Feher G. Structure of the reaction center from Rhodobacter sphaeroides R-26: membrane-protein interactions. Proc Natl Acad Sci U S A. 1987;84:6438-42 pubmed
    ..The electrostatic potential of the cofactors was calculated. The results show an asymmetry in the potential between the two possible pathways of electron transfer, with the A branch being preferred electrostatically...
  52. Abresch E, Paddock M, Villalobos M, Chang C, Okamura M. Interaction between cytochrome c2 and the photosynthetic reaction center from Rhodobacter sphaeroides: role of interprotein hydrogen bonds in binding and electron transfer. Biochemistry. 2008;47:13318-25 pubmed publisher
    ..The close packing contributes to fast electron transfer by increasing the rate of electronic coupling and contributes to the binding energy holding the cyt in position for times sufficient for electron transfer to occur. ..
  53. Nam T, ZIEGELHOFFER E, Lemke R, Donohue T. Proteins needed to activate a transcriptional response to the reactive oxygen species singlet oxygen. MBio. 2013;4:e00541-12 pubmed publisher
    ..Thus, our findings provide new information about a stress response to a ROS of broad biological, agricultural, and biomedical importance...
  54. Coomber S, Jones R, Jordan P, Hunter C. A putative anaerobic coproporphyrinogen III oxidase in Rhodobacter sphaeroides. I. Molecular cloning, transposon mutagenesis and sequence analysis of the gene. Mol Microbiol. 1992;6:3159-69 pubmed
    ..Sequence and transcription analysis defined the position and expression of an open reading frame of approximately 920 bp, which is proposed as the anaerobic coproporphyrinogen III oxidase dedicated to bacteriochlorophyll biosynthesis. ..
  55. Benning C, Somerville C. Isolation and genetic complementation of a sulfolipid-deficient mutant of Rhodobacter sphaeroides. J Bacteriol. 1992;174:2352-60 pubmed
    ..The putative sqdA gene product is a protein with a molecular mass of 33.6 kDa that has no sequence similarity to any enzyme of known function. ..
  56. Donohue T, McEwan A, Kaplan S. Cloning, DNA sequence, and expression of the Rhodobacter sphaeroides cytochrome c2 gene. J Bacteriol. 1986;168:962-72 pubmed
    ..Our results suggest that the increase in the cellular level of the cytochrome c2 protein found in photosynthetic cells was due, in part, to increased transcription of the single-copy cyc operon...
  57. Lang H, Cogdell R, Takaichi S, Hunter C. Complete DNA sequence, specific Tn5 insertion map, and gene assignment of the carotenoid biosynthesis pathway of Rhodobacter sphaeroides. J Bacteriol. 1995;177:2064-73 pubmed
  58. Calero S, Fernandez de Henestrosa A, Barbe J. Molecular cloning, sequence and regulation of expression of the recA gene of the phototrophic bacterium Rhodobacter sphaeroides. Mol Gen Genet. 1994;242:116-20 pubmed
    ..This is the first recA gene from a Gram-negative bacterium that lacks an E. coli-like SOS box but whose expression has been shown to be DNA damage-inducible and auto-regulated. ..
  59. McGlynn P, Hunter C. Genetic analysis of the bchC and bchA genes of Rhodobacter sphaeroides. Mol Gen Genet. 1993;236:227-34 pubmed
    ..Similarity between the putative BchX protein and dinitrogenase reductase proteins suggests that BchX may also be a reductase, supplying electrons for reduction of 2-devinyl-2-hydroxyethyl chlorophyllide a...
  60. Dryden S, Dowhan W. Isolation and expression of the Rhodobacter sphaeroides gene (pgsA) encoding phosphatidylglycerophosphate synthase. J Bacteriol. 1996;178:1030-8 pubmed
    ..coli pgsA. Overexpression of PgsARs in either E. coli or R. sphaeroides did not have dramatic effects on the phospholipid composition of the cells, suggesting regulation of the activity of this enzyme in both organisms. ..
  61. Shimada H, Wada T, Handa H, Ohta H, Mizoguchi H, Nishimura K, et al. A transcription factor with a leucine-zipper motif involved in light-dependent inhibition of expression of the puf operon in the photosynthetic bacterium Rhodobacter sphaeroides. Plant Cell Physiol. 1996;37:515-22 pubmed
    ..Northern analysis indicated that spb was constitutively and monocistronically transcribed in R. sphaeroides, irrespective of growth conditions. Structural and functional differences between SPB and HvrA are discussed. ..
  62. Toledo Cuevas M, Barquera B, Gennis R, Wikstr m M, Garc a Horsman J. The cbb3-type cytochrome c oxidase from Rhodobacter sphaeroides, a proton-pumping heme-copper oxidase. Biochim Biophys Acta. 1998;1365:421-34 pubmed
    ..Analysis of proton translocation in several strains shows that cytochrome cbb3 is a proton pump. We also conclude that cytochromes cbb3 and aa3 are the only cytochrome c oxidases in the respiratory chain of R. sphaeroides...
  63. Conroy M, Westerhuis W, Parkes Loach P, Loach P, Hunter C, Williamson M. The solution structure of Rhodobacter sphaeroides LH1beta reveals two helical domains separated by a more flexible region: structural consequences for the LH1 complex. J Mol Biol. 2000;298:83-94 pubmed
    ..This modification puts the N terminus of LH1beta close to the reaction centre H subunit, and provides a rationale for the different ring sizes of LH1 and LH2 complexes...
  64. Uchino Y, Yokota A. "Green-like" and "red-like" RubisCO cbbL genes in Rhodobacter azotoformans. Mol Biol Evol. 2003;20:821-30 pubmed publisher
    ..azotoformans and R. sphaeroides subsequently obtained a red-like cbbL gene by a horizontal gene transfer, and the ancestor of R. sphaeroides later lost the green-like cbbL gene...
  65. Carter M, Alber B. Transcriptional Regulation by the Short-Chain Fatty Acyl Coenzyme A Regulator (ScfR) PccR Controls Propionyl Coenzyme A Assimilation by Rhodobacter sphaeroides. J Bacteriol. 2015;197:3048-56 pubmed publisher
    ..Overall, this study evaluates a single regulator of propionyl-CoA assimilation while expanding the knowledge of the regulation of short-chain acyl-CoAs in many bacterial species. ..
  66. Oliveira A, Campos S, Baptista A, Soares C. Coupling between protonation and conformation in cytochrome c oxidase: Insights from constant-pH MD simulations. Biochim Biophys Acta. 2016;1857:759-71 pubmed publisher
    ..The protonation state of these two groups is heavily influenced by subtle conformational changes in the protein (notably of R481(I) and R482(I)) and by small changes in the hydrogen bond network. ..
  67. Qian P, Martin E, Ng I, Hunter C. The C-terminus of PufX plays a key role in dimerisation and assembly of the reaction center light-harvesting 1 complex from Rhodobacter sphaeroides. Biochim Biophys Acta Bioenerg. 2017;1858:795-803 pubmed publisher
    ..On the basis of the insights gained from these mutagenesis and structural analyses we propose a sequence for assembly of the dimeric RC-LH1-PufX complex. ..
  68. Allen J, Feher G. Crystallization of reaction center from Rhodopseudomonas sphaeroides: preliminary characterization. Proc Natl Acad Sci U S A. 1984;81:4795-9 pubmed
    ..The two bands at 535 and 544 nm assigned to the Qx transitions of the bacteriopheophytins were resolved and preliminary orientations of some of the pigments were obtained. ..
  69. Schindelin H, Kisker C, Hilton J, Rajagopalan K, Rees D. Crystal structure of DMSO reductase: redox-linked changes in molybdopterin coordination. Science. 1996;272:1615-21 pubmed
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    ..sphaeroides and also imply that a straight hook, or perhaps the rigidity associated with this feature, is important for an efficient swimming behavior in this bacterium...
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    ..A model will be presented to explain the role of transhydrogenase under aerobic conditions when cells need glutathione both for GSH-FDH activity and to repair oxidatively damaged proteins...
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    ..Therefore, the AppA/PpsR system was unable to establish light signaling in other bacteria. Based on our data, we present a model for the interdependence of AppA/PpsR signaling and the PrrA transcriptional activator...
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    ..The intermediates and partial electrogenic steps observed in the single-electron photoreduction experiments may be very different from those observed during oxidation of the fully reduced oxidase by O(2) in the "flow-flash" studies. ...
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    ..Finally, we demonstrate that the tautomerized glutamine is consistent with our criteria and observations of the BLUF light state...
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    ..This model may prove useful for determining the mode of PpsR action...
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    ..We propose that the blue light signal is transmitted towards the surface of the BLUF domain via His44, which serves as a reporter of active site changes...