Rhodobacter capsulatus SB 1003


Alias: Rhodobacter capsulatus SB1003, Rhodobacter capsulatus str. SB 1003, Rhodobacter capsulatus strain SB 1003

Top Publications

  1. Pawlik G, Kulajta C, Sachelaru I, Schröder S, Waidner B, Hellwig P, et al. The putative assembly factor CcoH is stably associated with the cbb3-type cytochrome oxidase. J Bacteriol. 2010;192:6378-89 pubmed publisher
    ..Thus, CcoH behaves more like a bona fide subunit of the cbb(3)-Cox than an assembly factor per se. ..
  2. Bauer C, Young D, Marrs B. Analysis of the Rhodobacter capsulatus puf operon. Location of the oxygen-regulated promoter region and the identification of an additional puf-encoded gene. J Biol Chem. 1988;263:4820-7 pubmed
    ..Finally, we also demonstrate that puf operon transcription initiation and regulation does not involve any puf-encoded gene products. ..
  3. Fujita Y, Bauer C. Reconstitution of light-independent protochlorophyllide reductase from purified bchl and BchN-BchB subunits. In vitro confirmation of nitrogenase-like features of a bacteriochlorophyll biosynthesis enzyme. J Biol Chem. 2000;275:23583-8 pubmed
    ..Dark protochlorophyllide reductase activity was shown to be dependent on the presence of all three subunits, ATP, and the reductant dithionite. The similarity of dark protochlorophyllide reductase to nitrogenase is discussed. ..
  4. Burke D, Alberti M, Hearst J. The Rhodobacter capsulatus chlorin reductase-encoding locus, bchA, consists of three genes, bchX, bchY, and bchZ. J Bacteriol. 1993;175:2407-13 pubmed
  5. Jones R, Haselkorn R. The DNA sequence of the Rhodobacter capsulatus ntrA, ntrB and ntrC gene analogues required for nitrogen fixation. Mol Gen Genet. 1989;215:507-16 pubmed
    ..The two-component regulatory system of ntrB and ntrC in E. coli is represented by nifR2 and nifR1 in R. capsulatus and nifR4 in R. capsulatus is the equivalent of the E. coli ntr-related sigma factor ntrA. ..
  6. Bartley G, Schmidhauser T, Yanofsky C, Scolnik P. Carotenoid desaturases from Rhodobacter capsulatus and Neurospora crassa are structurally and functionally conserved and contain domains homologous to flavoprotein disulfide oxidoreductases. J Biol Chem. 1990;265:16020-4 pubmed
    ..Our results indicate that both structure and function of carotenoid desaturases have been conserved during evolution and suggest that these enzymes are evolutionarily related to disulfide oxidoreductases. ..
  7. Beckman D, Trawick D, Kranz R. Bacterial cytochromes c biogenesis. Genes Dev. 1992;6:268-83 pubmed
    ..Taken together, these results support the hypothesis that the hel-encoded proteins are required for the export of heme to the periplasm where it is subsequently ligated to the c-type apocytochromes. ..
  8. Saeki K, Miyatake Y, Young D, Marrs B, Matsubara H. A plant-ferredoxin-like gene is located upstream of ferredoxin I gene (fdxN) of Rhodobacter capsulatus. Nucleic Acids Res. 1990;18:1060 pubmed
  9. Saeki K, Tokuda K, Fujiwara T, Matsubara H. Nucleotide sequence and genetic analysis of the region essential for functional expression of the gene for ferredoxin I, fdxN, in Rhodobacter capsulatus: sharing of one upstream activator sequence in opposite directions by two operons related to nitr. Plant Cell Physiol. 1993;34:185-99 pubmed

More Information


  1. Buggy J, Sganga M, Bauer C. Nucleotide sequence and characterization of the Rhodobacter capsulatus hvrB gene: HvrB is an activator of S-adenosyl-L-homocysteine hydrolase expression and is a member of the LysR family. J Bacteriol. 1994;176:61-9 pubmed
    ..capsulatus promoter elements. Our mutational analysis further demonstrates that hvrB autoregulates its own expression in vivo. ..
  2. Burke D, Alberti M, Hearst J. bchFNBH bacteriochlorophyll synthesis genes of Rhodobacter capsulatus and identification of the third subunit of light-independent protochlorophyllide reductase in bacteria and plants. J Bacteriol. 1993;175:2414-22 pubmed
    ..The further similarities of all three subunits of protochlorophyllide reductase and the three subunits of chlorin reductase in bacteriochlorophyll synthesis suggest that the two reductase systems are derived from a common ancestor. ..
  3. Hornberger U, Liebetanz R, Tichy H, Drews G. Cloning and sequencing of the hemA gene of Rhodobacter capsulatus and isolation of a delta-aminolevulinic acid-dependent mutant strain. Mol Gen Genet. 1990;221:371-8 pubmed
    ..capsulatus. Transfer of the R' factor pRPS404 and hybridization analysis revealed that the ALAS gene is not located within the major photosynthetic gene cluster. ..
  4. Yang Z, Bauer C. Rhodobacter capsulatus genes involved in early steps of the bacteriochlorophyll biosynthetic pathway. J Bacteriol. 1990;172:5001-10 pubmed
    ..The open reading frames were found to be part of a large 11-kilobase operon that encodes numerous genes involved in early steps of the bacteriochlorophyll a biosynthetic pathway. ..
  5. Saeki K, Suetsugu Y, Tokuda K, Miyatake Y, Young D, Marrs B, et al. Genetic analysis of functional differences among distinct ferredoxins in Rhodobacter capsulatus. J Biol Chem. 1991;266:12889-95 pubmed
    ..These indicate functional differences among multiple ferredoxins in one bacterium other than in cyanobacterial heterocysts and indispensability of certain ferredoxins in nitrogen fixation other than Rhizobium meliloti FdxN. ..
  6. Wright M, Eckert J, Biel S, Biel A. Use of a lacZ fusion to study transcriptional regulation of the Rhodobacter capsulatus hemA gene. FEMS Microbiol Lett. 1991;62:339-42 pubmed
    ..The unexpectedly small change in beta-galactosidase levels suggests that transcriptional regulation of the hemA gene is not the major factor in oxygen-mediated control of porphyrin synthesis. ..
  7. Zheng S, Haselkorn R. A glutamate/glutamine/aspartate/asparagine transport operon in Rhodobacter capsulatus. Mol Microbiol. 1996;20:1001-11 pubmed
  8. Badenhop F, Steiger S, Sandmann M, Sandmann G. Expression and biochemical characterization of the 1-HO-carotenoid methylase CrtF from Rhodobacter capsulatus. FEMS Microbiol Lett. 2003;222:237-42 pubmed
    ..capsulatus but also carotenoid intermediates leading to the formation of spirilloxanthin in a pathway which is absent in R. capsulatus but present in related species. ..
  9. Wiethaus J, Schubert B, Pfänder Y, Narberhaus F, Masepohl B. The GntR-like regulator TauR activates expression of taurine utilization genes in Rhodobacter capsulatus. J Bacteriol. 2008;190:487-93 pubmed
    ..In contrast, all other members of the GntR family analyzed so far bind to inverted repeats. ..
  10. Foster Hartnett D, Cullen P, Gabbert K, Kranz R. Sequence, genetic, and lacZ fusion analyses of a nifR3-ntrB-ntrC operon in Rhodobacter capsulatus. Mol Microbiol. 1993;8:903-14 pubmed
    ..This nifR3 strain is able to fix nitrogen and activate nifA1 and nifA2 genes, again supporting the hypothesis that nifR3 is not stringently required for ntrC-dependent gene activation in R. capsulatus. ..
  11. Ozturk M, Mandaci S. Two conserved non-canonical histidines are essential for activity of the cbb (3)-type oxidase in Rhodobacter capsulatus: non-canonical histidines are essential for cbb (3)-type oxidase activity in R. capsulatus. Mol Biol Rep. 2007;34:165-72 pubmed
  12. Jones R, Haselkorn R. The DNA sequence of the Rhodobacter capsulatus nifH gene. Nucleic Acids Res. 1988;16:8735 pubmed
  13. Davidson E, Daldal F. Primary structure of the bc1 complex of Rhodopseudomonas capsulata. Nucleotide sequence of the pet operon encoding the Rieske cytochrome b, and cytochrome c1 apoproteins. J Mol Biol. 1987;195:13-24 pubmed
    ..The amino acid sequences of the pet proteins are discussed with reference to the structure and function of the ubiquinol-cytochrome c2 oxidoreductase. ..
  14. Saeki K, Suetsugu Y, Yao Y, Horio T, Marrs B, Matsubara H. Two distinct ferredoxins from Rhodobacter capsulatus: complete amino acid sequences and molecular evolution. J Biochem. 1990;108:475-82 pubmed
    Two distinct ferredoxins were purified from Rhodobacter capsulatus SB1003. Their complete amino acid sequences were determined by a combination of protease digestion, BrCN cleavage and Edman degradation...
  15. Gray K, Grooms M, Myllykallio H, Moomaw C, Slaughter C, Daldal F. Rhodobacter capsulatus contains a novel cb-type cytochrome c oxidase without a CuA center. Biochemistry. 1994;33:3120-7 pubmed
    ..Pyridine hemochrome and HPLC analyses suggest that the complex contains 1 mol of heme C to 1 mol of protoheme and that neither heme O nor heme A is present.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  16. Beckman D, Kranz R. A bacterial homolog to HPRT. Biochim Biophys Acta. 1991;1129:112-4 pubmed
    ..This similarity includes conserved amino acid residues involved in Lesch-Nyhan syndrome. ..
  17. Becker Rudzik M, Young D, Marrs B. Sequence of the indoleglycerol phosphate synthase (trpC) gene from Rhodobacter capsulatus. J Bacteriol. 1992;174:5482-4 pubmed
    ..Normalized alignment scores comparing the trpC gene of R. capsulatus with the trpC genes of other bacterial species are reported. An unexpected degree of similarity to the trpC gene of Bacillus subtilis was found. ..
  18. Beckman D, Kranz R. A bacterial homolog to the mitochondrial enoyl-CoA hydratase. Gene. 1991;107:171-2 pubmed
    ..This similarity in size and sequence suggests that R. capsulatus oxidizes fatty acids using specific components, more like the mitochondrial system than the multifunctional component system of Escherichia coli. ..
  19. Muraki N, Nomata J, Ebata K, Mizoguchi T, Shiba T, Tamiaki H, et al. X-ray crystal structure of the light-independent protochlorophyllide reductase. Nature. 2010;465:110-4 pubmed publisher
  20. Astner I, Schulze J, van den Heuvel J, Jahn D, Schubert W, Heinz D. Crystal structure of 5-aminolevulinate synthase, the first enzyme of heme biosynthesis, and its link to XLSA in humans. EMBO J. 2005;24:3166-77 pubmed publisher
    ..Mutations are found to obstruct substrate binding, disrupt the dimer interface, or hamper the correct folding. The structure of ALAS completes the structural analysis of enzymes in heme biosynthesis...
  21. Deery E, Schroeder S, Lawrence A, Taylor S, Seyedarabi A, Waterman J, et al. An enzyme-trap approach allows isolation of intermediates in cobalamin biosynthesis. Nat Chem Biol. 2012;8:933-40 pubmed publisher
    ..The tight association of pathway intermediates with enzymes provides evidence for a form of metabolite channeling...
  22. Gerjets T, Steiger S, Sandmann G. Catalytic properties of the expressed acyclic carotenoid 2-ketolases from Rhodobacter capsulatus and Rubrivivax gelatinosus. Biochim Biophys Acta. 2009;1791:125-31 pubmed publisher
    ..capsulatus from which spirilloxanthin synthesis is absent. In general, the ketolase of Rvi. gelatinosus had a better specificity for spheroidene, HO-spheroidene and spirilloxanthin as substrates than the ketolase from Rba. capsulatus...
  23. Koch H, Hwang O, Daldal F. Isolation and characterization of Rhodobacter capsulatus mutants affected in cytochrome cbb3 oxidase activity. J Bacteriol. 1998;180:969-78 pubmed
  24. Lee P, Holtzapple E, Schmidt Dannert C. Novel activity of Rhodobacter sphaeroides spheroidene monooxygenase CrtA expressed in Escherichia coli. Appl Environ Microbiol. 2010;76:7328-31 pubmed publisher
    ..The heme-containing CrtA is not a P450 enzyme but a new type of oxygenase...
  25. Kumagai H, Fujiwara T, Matsubara H, Saeki K. Membrane localization, topology, and mutual stabilization of the rnfABC gene products in Rhodobacter capsulatus and implications for a new family of energy-coupling NADH oxidoreductases. Biochemistry. 1997;36:5509-21 pubmed publisher
    ..We predict that the putative Rnf complex represents a novel family of energy-coupling NADH oxidoreductases...
  26. Armstrong G, Alberti M, Leach F, Hearst J. Nucleotide sequence, organization, and nature of the protein products of the carotenoid biosynthesis gene cluster of Rhodobacter capsulatus. Mol Gen Genet. 1989;216:254-68 pubmed
    ..We have also observed two regions of exceptional amino acid homology between CrtI and CrtD, both of which are dehydrogenases...
  27. Beckman D, Kranz R. Cytochromes c biogenesis in a photosynthetic bacterium requires a periplasmic thioredoxin-like protein. Proc Natl Acad Sci U S A. 1993;90:2179-83 pubmed
    ..This role is in accordance with the observation that both heme and the cysteines of apocytochromes c (Cys-Xaa-Yaa-Cys-His) must be in the reduced state for covalent linkage between the two moieties to occur...
  28. Peters A, Kulajta C, Pawlik G, Daldal F, Koch H. Stability of the cbb3-type cytochrome oxidase requires specific CcoQ-CcoP interactions. J Bacteriol. 2008;190:5576-86 pubmed publisher
    ..capsulatus CcoQ was required for optimal cbb(3)-type oxidase activity because it stabilized the interaction of CcoP with the CcoNO core complex, leading subsequently to the formation of the active 230-kDa cbb(3)-type oxidase complex...
  29. Nomata J, Ogawa T, Kitashima M, Inoue K, Fujita Y. NB-protein (BchN-BchB) of dark-operative protochlorophyllide reductase is the catalytic component containing oxygen-tolerant Fe-S clusters. FEBS Lett. 2008;582:1346-50 pubmed publisher
    ..Together with the Fe and sulfide contents, these findings suggested that NB-protein carries two oxygen-tolerant [4Fe-4S] clusters...
  30. Paoli G, Morgan N, Tabita F, Shively J. Expression of the cbbLcbbS and cbbM genes and distinct organization of the cbb Calvin cycle structural genes of Rhodobacter capsulatus. Arch Microbiol. 1995;164:396-405 pubmed
    ..Complementation of a RubisCO-deletion strain of R. sphaeroides to photosynthetic growth by R. capsulatus cbbLcbbS or cbbM was achieved using the broad host-range vector, pRK415, and R. sphaeroides expression vector pRPS-1...
  31. Nomata J, Mizoguchi T, Tamiaki H, Fujita Y. A second nitrogenase-like enzyme for bacteriochlorophyll biosynthesis: reconstitution of chlorophyllide a reductase with purified X-protein (BchX) and YZ-protein (BchY-BchZ) from Rhodobacter capsulatus. J Biol Chem. 2006;281:15021-8 pubmed publisher
    ..The evolutionary implications of nitrogenase-like enzymes to determine the ring structure of (bacterio)chlorophyll pigments are discussed...
  32. Gough S, Petersen B, Duus J. Anaerobic chlorophyll isocyclic ring formation in Rhodobacter capsulatus requires a cobalamin cofactor. Proc Natl Acad Sci U S A. 2000;97:6908-13 pubmed
    ..Withdrawal of an electron gives the 13(1)-cation of MPE. Hydroxyl ion attack on the cation gives 13(1)-hydroxy-MPE. Withdrawal of three hydrogen atoms leads successively to 13(1)-keto-MPE, its 13(2)-radical, and cyclization to PChlide...
  33. Deshmukh M, Brasseur G, Daldal F. Novel Rhodobacter capsulatus genes required for the biogenesis of various c-type cytochromes. Mol Microbiol. 2000;35:123-38 pubmed
    ..This study demonstrated for the first time that CcdA homologues are also required for cyt c biogenesis in some gram-negative bacteria such as R. capsulatus...
  34. Cordier F, Caffrey M, Brutscher B, Cusanovich M, Marion D, Blackledge M. Solution structure, rotational diffusion anisotropy and local backbone dynamics of Rhodobacter capsulatus cytochrome c2. J Mol Biol. 1998;281:341-61 pubmed publisher
    ..While the molecule is essentially rigid, a tripeptide loop region (residues 101 to 103) exhibits flexibility in the range of 20 to 30 ps, which appears to be correlated with the order in the NMR solution structure...
  35. Paoli G, Vichivanives P, Tabita F. Physiological control and regulation of the Rhodobacter capsulatus cbb operons. J Bacteriol. 1998;180:4258-69 pubmed
    ..capsulatus are within separate CbbR regulons...
  36. Paoli G, Soyer F, Shively J, Tabita F. Rhodobacter capsulatus genes encoding form I ribulose-1,5-bisphosphate carboxylase/oxygenase (cbbLS) and neighbouring genes were acquired by a horizontal gene transfer. Microbiology. 1998;144 ( Pt 1):219-27 pubmed publisher
    ..These studies suggest that the cbbRI-cbbL-cbbS-cbbQ genes were acquired by Rh. capsulatus via horizontal gene transfer from a bacterial species containing a 'green-like' RubisCO...
  37. Sganga M, Aksamit R, Cantoni G, Bauer C. Mutational and nucleotide sequence analysis of S-adenosyl-L-homocysteine hydrolase from Rhodobacter capsulatus. Proc Natl Acad Sci U S A. 1992;89:6328-32 pubmed
  38. Tokito M, Daldal F. petR, located upstream of the fbcFBC operon encoding the cytochrome bc1 complex, is homologous to bacterial response regulators and necessary for photosynthetic and respiratory growth of Rhodobacter capsulatus. Mol Microbiol. 1992;6:1645-54 pubmed
    ..Moreover, it was found that PetR mutants are unable to grow on rich or minimal media by either photosynthesis or respiration, demonstrating that these gene products are essential for growth of R. capsulatus...
  39. Rodionov D, Hebbeln P, Gelfand M, Eitinger T. Comparative and functional genomic analysis of prokaryotic nickel and cobalt uptake transporters: evidence for a novel group of ATP-binding cassette transporters. J Bacteriol. 2006;188:317-27 pubmed
    ..Experimental analysis confirmed metal transport activity for three members of this family and demonstrated significant activity for a basic module (CbiMN) of the Salmonella enterica serovar Typhimurium transporter...
  40. Hebbeln P, Rodionov D, Alfandega A, Eitinger T. Biotin uptake in prokaryotes by solute transporters with an optional ATP-binding cassette-containing module. Proc Natl Acad Sci U S A. 2007;104:2909-14 pubmed
    ..The results substantiate our earlier suggestion of a mechanistically novel group of membrane transporters...
  41. Goldman B, Beckman D, Bali A, Monika E, Gabbert K, Kranz R. Molecular and immunological analysis of an ABC transporter complex required for cytochrome c biogenesis. J Mol Biol. 1997;268:724-38 pubmed
    ..Thus, the HelABCD heme export complex is distinguished by the presence of four membrane-associated subunits and represents a unique subfamily of ABC transporters...
  42. Siche S, Neubauer O, Hebbeln P, Eitinger T. A bipartite S unit of an ECF-type cobalt transporter. Res Microbiol. 2010;161:824-9 pubmed publisher
    ..Specifically, they point to essential roles of His? and the distance of His? to the amino group of the peptide chain in metal recognition...