Rhizobium

Summary

Alias: Allorhizobium, Allorhizobium de Lajudie et al. 1998, "Phytomyxa" Schroeter 1886, "Rhizobacterium" Kirchner 1896, Phytomyxa, Rhizobacterium, Rhizobium Frank 1889 (Approved Lists 1980) emend. Young et al. 2001

Top Publications

  1. ncbi Analysis of cellular fatty acids and phenotypic relationships of Agrobacterium, Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium species using the Sherlock Microbial Identification System
    S W Tighe
    Analytical Services, Inc, Williston, VT, USA
    Int J Syst Evol Microbiol 50:787-801. 2000
  2. pmc The Rhizobium etli rpoN locus: DNA sequence analysis and phenotypical characterization of rpoN, ptsN, and ptsA mutants
    J Michiels
    F A Janssens Laboratory of Genetics, K U Leuven, Heverlee, Belgium
    J Bacteriol 180:1729-40. 1998
  3. ncbi Changing concepts in the systematics of bacterial nitrogen-fixing legume symbionts
    Hiroyucki Sawada
    National Institute for Agro Environmental Sciences, Tsukuba 305 8604, Japan
    J Gen Appl Microbiol 49:155-79. 2003
  4. pmc Structures of the lipopolysaccharides from Rhizobium leguminosarum RBL5523 and its UDP-glucose dehydrogenase mutant (exo5)
    Artur Muszynski
    Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA
    Glycobiology 21:55-68. 2011
  5. ncbi A special acyl carrier protein for transferring long hydroxylated fatty acids to lipid A in Rhizobium
    K A Brozek
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 271:32126-36. 1996
  6. pmc A high-throughput transient gene expression system for switchgrass (Panicum virgatum L.) seedlings
    Xinlu Chen
    University of Tennessee, Department of Plant Sciences, 2431 Joe Johnson Drive, Knoxville, TN, 37996, USA
    Biotechnol Biofuels 3:9. 2010
  7. ncbi [Current concepts on the pathogenicity of phytopathogenic bacteria]
    C Boucher
    Laboratoire de biologie moléculaire des interactions plantes micro organismes, INRA CNRS, BP 27, 31326 Castanet Tolosan, France
    C R Acad Sci III 324:915-22. 2001
  8. pmc Expression cloning and characterization of the C28 acyltransferase of lipid A biosynthesis in Rhizobium leguminosarum
    Shib Sankar Basu
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 277:28959-71. 2002
  9. ncbi Rhizobium gallicum sp. nov. and Rhizobium giardinii sp. nov., from Phaseolus vulgaris nodules
    N Amarger
    Laboratoire de Microbiologie des Sols, Centre de Microbiologie du Sol et de L Environnement, INRA, Dijon, France
    Int J Syst Bacteriol 47:996-1006. 1997
  10. ncbi A homolog of the Rhizobium meliloti nitrogen fixation gene fixN is involved in the production of a microaerobically induced oxidase activity in the phytopathogenic bacterium Agrobacterium tumefaciens
    A Schluter
    Okologie des Bodens, Botanisches Institut, RWTH Aachen, Germany
    Mol Gen Genet 247:206-15. 1995

Detail Information

Publications441 found, 100 shown here

  1. ncbi Analysis of cellular fatty acids and phenotypic relationships of Agrobacterium, Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium species using the Sherlock Microbial Identification System
    S W Tighe
    Analytical Services, Inc, Williston, VT, USA
    Int J Syst Evol Microbiol 50:787-801. 2000
    ..fatty acid profiles of over 600 strains belonging to the genera Agrobacterium, Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium were evaluated using the gaschromatography-based Sherlock Microbial Identification System (MIS)..
  2. pmc The Rhizobium etli rpoN locus: DNA sequence analysis and phenotypical characterization of rpoN, ptsN, and ptsA mutants
    J Michiels
    F A Janssens Laboratory of Genetics, K U Leuven, Heverlee, Belgium
    J Bacteriol 180:1729-40. 1998
    The rpoN region of Rhizobium etli was isolated by using the Bradyrhizobium japonicum rpoN1 gene as a probe...
  3. ncbi Changing concepts in the systematics of bacterial nitrogen-fixing legume symbionts
    Hiroyucki Sawada
    National Institute for Agro Environmental Sciences, Tsukuba 305 8604, Japan
    J Gen Appl Microbiol 49:155-79. 2003
    ..taxa that do not contain legume symbionts (shown in parentheses below): Group 1, which comprises Rhizobium and Allorhizobium species containing legume symbionts (intermingled with Agrobacterium and Blastobacter species, which are ..
  4. pmc Structures of the lipopolysaccharides from Rhizobium leguminosarum RBL5523 and its UDP-glucose dehydrogenase mutant (exo5)
    Artur Muszynski
    Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA
    Glycobiology 21:55-68. 2011
    ..An exo5 mutant of Rhizobium leguminosarum RBL5523, strain RBL5808, is defective in UDP-glucose (Glc) dehydrogenase that converts UDP-Glc to ..
  5. ncbi A special acyl carrier protein for transferring long hydroxylated fatty acids to lipid A in Rhizobium
    K A Brozek
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 271:32126-36. 1996
    ..The Rhizobium meliloti lipid A backbone, like that of Escherichia coli, is a beta1'-6-linked glucosamine disaccharide that is ..
  6. pmc A high-throughput transient gene expression system for switchgrass (Panicum virgatum L.) seedlings
    Xinlu Chen
    University of Tennessee, Department of Plant Sciences, 2431 Joe Johnson Drive, Knoxville, TN, 37996, USA
    Biotechnol Biofuels 3:9. 2010
    ..Experiments to increase transient gene expression in planta may lead to stable transformation methods with increased efficiency...
  7. ncbi [Current concepts on the pathogenicity of phytopathogenic bacteria]
    C Boucher
    Laboratoire de biologie moléculaire des interactions plantes micro organismes, INRA CNRS, BP 27, 31326 Castanet Tolosan, France
    C R Acad Sci III 324:915-22. 2001
    ..Identification of the effector proteins, of their function and of the corresponding molecular targets in the host is a new challenge which will contribute to the conception of new strategies to control diseases...
  8. pmc Expression cloning and characterization of the C28 acyltransferase of lipid A biosynthesis in Rhizobium leguminosarum
    Shib Sankar Basu
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 277:28959-71. 2002
    ..An enzyme that incorporates this acyl chain is present in extracts of Rhizobium leguminosarum, Rhizobium etli, and Sinorhizobium meliloti but not Escherichia coli...
  9. ncbi Rhizobium gallicum sp. nov. and Rhizobium giardinii sp. nov., from Phaseolus vulgaris nodules
    N Amarger
    Laboratoire de Microbiologie des Sols, Centre de Microbiologie du Sol et de L Environnement, INRA, Dijon, France
    Int J Syst Bacteriol 47:996-1006. 1997
    ..Genomic species 1 clustered with the species that include the bean-nodulating rhizobia, Rhizobium leguminosarum, Rhizobium etli, and Rhizobium tropici, and branched with unclassified rhizobial strain OK50, ..
  10. ncbi A homolog of the Rhizobium meliloti nitrogen fixation gene fixN is involved in the production of a microaerobically induced oxidase activity in the phytopathogenic bacterium Agrobacterium tumefaciens
    A Schluter
    Okologie des Bodens, Botanisches Institut, RWTH Aachen, Germany
    Mol Gen Genet 247:206-15. 1995
    Hybridization analysis using the Rhizobium meliloti nitrogen fixation gene fixN as a probe revealed the presence of a homologous DNA region in the phytopathogenic bacterium Agrobacterium tumefaciens...
  11. pmc Cloning, sequencing, and characterization of the cgmB gene of Sinorhizobium meliloti involved in cyclic beta-glucan biosynthesis
    P Wang
    Department of Food Science, The Pennsylvania State University, University Park, Pennsylvania, USA
    J Bacteriol 181:4576-83. 1999
    Periplasmic cyclic beta-glucans of Rhizobium species provide important functions during plant infection and hypo-osmotic adaptation...
  12. doi Agrobacterium-mediated transformation of embryogenic cultures and plant regeneration in Vitis rotundifolia Michx. (muscadine grape)
    S A Dhekney
    Mid Florida Research and Education Center, University of Florida IFAS, 2725 Binion Road, Apopka, FL 32703 8504, USA
    Plant Cell Rep 27:865-72. 2008
    ..Transgenic plants exhibited uniform GFP expression in cells of all plant tissues and organs including leaves, stems, roots, inflorescences and the embryo and endosperm of developing berries...
  13. pmc Cloning and characterization of a tetracycline resistance determinant present in Agrobacterium tumefaciens C58
    Z Q Luo
    Departments of Crop Sciences, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    J Bacteriol 181:618-26. 1999
    ..While the repressive effect of the TetR protein from strain C58 (TetRC58) on the tetA gene from strain RP4 (tetARP4) was not relieved by tetracycline, repression of tetAC58 by TetRRP4 was lifted by this antibiotic...
  14. ncbi Colonization and nitrogenase activity of Triticum aestivum (cv. Baccross and Mahdavi) to the dual inoculation with Azospirillum brasilense and Rhizobium meliloti plus 2,4-D
    Askary Mehry
    Department of Biology, University of Arak, Arak, Iran
    Pak J Biol Sci 11:1541-50. 2008
    ..was studied with application of two Azospirillum brasilense strains (native and Sp7) co-inoculated with two Rhizobium meliloti strains (native and DSMZ 30135)...
  15. pmc Genes essential for nod factor production and nodulation are located on a symbiotic amplicon (AMPRtrCFN299pc60) in Rhizobium tropici
    P Mavingui
    Centro de Investigacion Sobre Fijacion de Nitrogeno, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico
    J Bacteriol 180:2866-74. 1998
    Amplifiable DNA regions (amplicons) have been identified in the genome of Rhizobium etli. Here we report the isolation and molecular characterization of a symbiotic amplicon of Rhizobium tropici...
  16. ncbi Regulation of the TCA cycle and the general amino acid permease by overflow metabolism in Rhizobium leguminosarum
    D L Walshaw
    School of Animal and Microbial Sciences, University of Reading, Whiteknights, UK
    Microbiology 143:2209-21. 1997
    Mutants of Rhizobium leguminosarum were selected that were altered in the uptake activity of the general amino acid permease (Aap)...
  17. pmc Identification of strains of Alcaligenes and Agrobacterium by a polyphasic approach
    D Clermont
    Collection de l Institut Pasteur, 75724 Paris Cedex 15, France
    J Clin Microbiol 39:3104-9. 2001
    ..xylosoxidans subspecies and that these two methods can be used as alternative methods to characterize unidentified strains rapidly when discriminant biochemical characters are missing...
  18. ncbi Complete amino acid sequence and characterization of the reaction mechanism of a glucosamine-induced novel alcohol dehydrogenase from Agrobacterium radiobacter (tumefaciens)
    Ryoko Iwamoto
    Department of Chemistry, Nara Women s University, Kitauoya Nishimachi, Nara, 630 8506, Japan
    Arch Biochem Biophys 398:203-12. 2002
    ..0. (3) The rate-determining step of the dehydrogenase for ethanol is deprotonation of the enzyme-NAD-Zn-OHCH(2)CH(3) complex to enzyme-NAD-Zn-O(-)CH(2)CH(3) complex and that for glucosaminitol is H(2)O addition to enzyme-Zn-NADH complex...
  19. pmc Second site mutations specifically suppress the Fix- phenotype of Rhizobium meliloti ndvF mutations on alfalfa: identification of a conditional ndvF-dependent mucoid colony phenotype
    I J Oresnik
    Department of Biology, McMaster University, Hamilton, Ontario, Canada
    Genetics 136:1233-43. 1994
    b>Rhizobium meliloti mutants carrying ndvF insertion or deletion mutations induce nodules on alfalfa which contain very few infected cells and fail to fix N2 (Fix-)...
  20. doi Broad-range antagonistic rhizobacteria Pseudomonas fluorescens and Serratia plymuthica suppress Agrobacterium crown gall tumours on tomato plants
    N Dandurishvili
    Otto Warburg Center for Agricultural Biotechnology, The Robert H Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
    J Appl Microbiol 110:341-52. 2011
    ..To examine the biocontrol activity of broad-range antagonists Serratia plymuthica IC1270, Pseudomonas fluorescens Q8r1-96 and P. fluorescens B-4117 against tumourigenic strains of Agrobacterium tumefaciens and A. vitis...
  21. ncbi A new nos gene downstream from nosDFY is essential for dissimilatory reduction of nitrous oxide by Rhizobium (Sinorhizobium) meliloti
    Y K Chan
    Eastern Cereal and Oilseed Research Centre, Agriculture and Agri Food Canada, Central Experimental Farm, Ottawa, Ontario, Canada
    Microbiology 143:2817-24. 1997
    b>Rhizobium (Sinorhizobium) meliloti strains capable of dissimilatory nitrous oxide reduction (Nos+) carry a nosRZDFY gene cluster on a 10.1 kb EcoRI fragment of the nod megaplasmid near the fixGHIS genes...
  22. ncbi Role of cellulose fibrils and exopolysaccharides of Rhizobium leguminosarum in attachment to and infection of Vicia sativa root hairs
    M C Laus
    Institute of Biology Leiden, Leiden University, Wassenaarseweg 64, 2333AL Leiden, The Netherlands
    Mol Plant Microbe Interact 18:533-8. 2005
    Infection and subsequent nodulation of legume host plants by the root nodule symbiote Rhizobium leguminosarum usually require attachment of the bacteria to root-hair tips...
  23. ncbi Phylogenetic analysis of the genera Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium on the basis of 16S rRNA gene and internally transcribed spacer region sequences
    Soon Wo Kwon
    Korean Agricultural Culture Collection KACC, Genetic Resources Division, National Institute of Agricultural Biotechnology, Rural Development Administration RDA, Suwon 441 707, Korea
    Int J Syst Evol Microbiol 55:263-70. 2005
    ..relationships between these Korean isolates and reference strains of the genera Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium were analysed using their 16S rRNA gene and internally transcribed spacer (ITS) region ..
  24. doi First genomic analysis of the broad-host-range Rhizobium sp. LPU83 strain, a member of the low-genetic diversity Oregon-like Rhizobium sp. group
    Gonzalo Torres Tejerizo
    IBBM Instituto de Biotecnología y Biología Molecular, CCT CONICET La Plata, Departamento de Ciencias Biologicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115, 1900 La Plata, Argentina
    J Biotechnol 155:3-10. 2011
    ..Ensifer (Sinorhizobium) medicae and with less characterized types of rhizobia, such as the Oregon-like strains, Rhizobium sp. Or191 initially isolated in the USA, and the Rhizobium sp. LPU83 strain, from Argentina...
  25. doi Rhizobium fabae sp. nov., a bacterium that nodulates Vicia faba
    Chang Fu Tian
    Key Laboratory of Agro Microbial Resource and Application, Ministry of Agriculture College of Biological Sciences, China Agricultural University, Beijing, PR China
    Int J Syst Evol Microbiol 58:2871-5. 2008
    ..According to phylogenetic analyses of 16S rRNA gene, atpD and recA sequences, these strains belong to the genus Rhizobium, with Rhizobium etli and Rhizobium leguminosarum as the closest related species...
  26. pmc Rhizobium leguminosarum hupE encodes a nickel transporter required for hydrogenase activity
    Belen Brito
    Departamento de Biotecnologia, Escuela Tecnica Superior de Ingenieros Agronomos, Universidad Politecnica de Madrid, Madrid, Spain
    J Bacteriol 192:925-35. 2010
    Synthesis of the hydrogen uptake (Hup) system in Rhizobium leguminosarum bv. viciae requires the function of an 18-gene cluster (hupSLCDEFGHIJK-hypABFCDEX)...
  27. pmc The Agrobacterium rhizogenes GALLS gene encodes two secreted proteins required for genetic transformation of plants
    Larry D Hodges
    Department of Microbiology, Oregon State University, Corvallis, OR 97331, USA
    J Bacteriol 191:355-64. 2009
    ..In plant cells, the GALLS proteins interacted with themselves, VirD2, and each other. VirD2 interacted with GALLS-FL and localized inside the nucleus, where its predicted helicase activity may pull T strands into the nucleus...
  28. ncbi Evaluation of bean (Phaseolus vulgaris) seeds inoculation with Rhizobium phaseoli and plant growth promoting rhizobacteria on yield and yield components
    M Yadegari
    Islamic Azad University of Shahrekord Branch, Shahrekord, Iran
    Pak J Biol Sci 11:1935-9. 2008
    To study the effect of co-inoculation with plant growth-promoting rhizobacteria (PGPR) and Rhizobium, on yield and yield components of common bean (Phaseolus vulgaris L...
  29. pmc An alternative succinate (2-oxoglutarate) transport system in Rhizobium tropici is induced in nodules of Phaseolus vulgaris
    Silvia Batista
    Unidad de Microbiología Molecular, Instituto de Investigaciones Biologicas Clemente Estable, Av Italia 3318, Montevideo 11600, Uruguay
    J Bacteriol 191:5057-67. 2009
    ..A previously described dctA mutant of Rhizobium tropici CIAT899, strain GA1 (dctA), however, was unexpectedly still able to grow on succinate as a sole carbon ..
  30. ncbi Use of nodulation pattern, stress tolerance, nodC gene amplification, RAPD-PCR and RFLP-16S rDNA analysis to discriminate genotypes of Rhizobium leguminosarum biovar viciae
    Giancarlo Moschetti
    Dipartimento di Scienza degli Alimenti, Sezione di Microbiologia Agraria, Alimnentare ed Ambientale e di Igiene, Universita degli Studi di Napoli Federico II, I 80055 Portici, Italy
    Syst Appl Microbiol 28:619-31. 2005
    Twenty-seven new Rhizobium isolates were obtained from root nodules of wild and crop legumes belonging to the genera Vicia, Lathyrus and Pisum from different agroecological areas in central and southern Italy...
  31. doi High-efficiency Agrobacterium-mediated transformation of citrus via sonication and vacuum infiltration
    Maria Luiza Peixoto de Oliveira
    Plant Biology Department, Plant Tissue Culture Laboratory BIOAGRO, Federal University of Vicosa, Vicosa, MG, Brazil
    Plant Cell Rep 28:387-95. 2009
    ..The transformation frequencies achieved in this study (8.4% for 'Pineapple' sweet orange and 11.2% for 'Swingle' citrumelo) are the highest ones reported for both cultivars...
  32. ncbi [Characterization of root nodule and rhizobium of a Leguminosae ephemeral plant--Trigonella arcuata C. A. Mey in Xinjiang]
    Tianyu You
    Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China
    Wei Sheng Wu Xue Bao 48:917-23. 2008
    We studied root nodule proliferation, nodule microstructure, genetic cluster and stress resistance of the rhizobium of Trigonella arcuata.
  33. ncbi Identification and structure of the Rhizobium galegae common nodulation genes: evidence for horizontal gene transfer
    L Suominen
    Department of Applied Chemistry and Microbiology, Institute of Biotechnology, University of Helsinki, Biocenter 1, FIN 00014 Helsinki, Finland
    Mol Biol Evol 18:907-16. 2001
    ..Here, we present the organization and sequence of the common nod genes from Rhizobium galegae, a symbiotic member of the RHIZOBIACEAE: This species has an intriguing phylogenetic position, being ..
  34. ncbi Proposal of Pseudorhodobacter ferrugineus gen nov, comb nov, for a non-photosynthetic marine bacterium, Agrobacterium ferrugineum, related to the genus Rhodobacter
    Yoshihito Uchino
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    J Gen Appl Microbiol 48:309-19. 2002
    ..ferrugineus possesses an incomplete photosynthetic apparatus...
  35. doi Regulation of flagellar, motility and chemotaxis genes in Rhizobium leguminosarum by the VisN/R-Rem cascade
    Dinah D Tambalo
    Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada
    Microbiology 156:1673-85. 2010
    ..the regulatory roles of VisN, VisR and Rem in the expression of flagellar, motility and chemotaxis genes in Rhizobium leguminosarum biovar viciae strains VF39SM and 3841...
  36. ncbi Classification of Austrian rhizobia and the Mexican isolate FL27 obtained from Phaseolus vulgaris L. as Rhizobium gallicum
    A Sessitsch
    Soil Science Unit, FAO IAEA Agriculture and Biotechnology Laboratory, Agency s Laboratories, Seibersdorf, Austria
    Int J Syst Bacteriol 47:1097-101. 1997
    ..Analysis of the 16S rRNA genes revealed sequences almost identical to that of the Rhizobium gallicum type strain, R602sp, with a maximum of two nucleotide substitutions...
  37. ncbi The Rhizobium leguminosarum prsDE genes are required for secretion of several proteins, some of which influence nodulation, symbiotic nitrogen fixation and exopolysaccharide modification
    C Finnie
    Department of Genetics, John Innes Centre, Colney, Norwich, UK
    Mol Microbiol 25:135-46. 1997
    NodO is a secreted protein from Rhizobium leguminosarum bv. viciae with a role in signalling during legume nodulation. A Tn5-induced mutant was identified that was defective in NodO secretion...
  38. pmc Regulation of gene expression in response to oxygen in Rhizobium etli: role of FnrN in fixNOQP expression and in symbiotic nitrogen fixation
    O Lopez
    Departamento de Microbiologia Molecular, Instituto de Biotecnologia, UNAM, Cuernavaca, Morelos, 62250, Mexico
    J Bacteriol 183:6999-7006. 2001
    Previously, we reported finding duplicated fixNOQP operons in Rhizobium etli CFN42. One of these duplicated operons is located in the symbiotic plasmid (fixNOQPd), while the other is located in a cryptic plasmid (fixNOQPf)...
  39. ncbi Identification and characterization of plant genes involved in Agrobacterium-mediated plant transformation by virus-induced gene silencing
    Ajith Anand
    Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK 73402, USA
    Mol Plant Microbe Interact 20:41-52. 2007
    ..tumefaciens is simple, rapid, and robust and complements other currently used approaches...
  40. ncbi Transformation of ipecac (Cephaelis ipecacuanha) with Agrobacterium rhizogenes
    Kayo Yoshimatsu
    Tsukuba Medicinal Plant Research Station, National Institute of Health Sciences, Tsukuba, Ibaraki, Japan
    Planta Med 69:1018-23. 2003
    ..The roots cultured in the dark grew well in Gamborg B5 (B5) liquid medium containing 0.5 mg/L IBA and yielded 112 mg/L of cephaeline and 14 mg/L emetine after 8 weeks of culture...
  41. pmc Altered lipid A structures and polymyxin hypersensitivity of Rhizobium etli mutants lacking the LpxE and LpxF phosphatases
    Brian O Ingram
    Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    Biochim Biophys Acta 1801:593-604. 2010
    The lipid A of Rhizobium etli, a nitrogen-fixing plant endosymbiont, displays significant structural differences when compared to that of Escherichia coli. An especially striking feature of R...
  42. pmc Rhodobacter sphaeroides rdxA, a homolog of Rhizobium meliloti fixG, encodes a membrane protein which may bind cytoplasmic [4Fe-4S] clusters
    E L Neidle
    Department of Microbiology and Molecular Genetics, University of Texas Health Science Center, Houston 77225
    J Bacteriol 174:6444-54. 1992
    ..chromosomal gene, rdxA, which encodes a 52-kDa protein, was found to be homologous to fixG, the first gene of a Rhizobium meliloti nitrogen fixation operon on the pSym plasmid (D. Kahn, M. David, O. Domergue, M.-L. Daveran, J. Ghai, P...
  43. ncbi High efficiency transgene segregation in co-transformed maize plants using an Agrobacterium tumefaciens 2 T-DNA binary system
    Michael Miller
    Pioneer Hi Bred International Inc, Johnston, Iowa 50131, USA
    Transgenic Res 11:381-96. 2002
    ..A high efficiency system to segregate transgenes in co-transformed maize plants has now been demonstrated...
  44. ncbi The use of the phosphomannose isomerase gene as alternative selectable marker for Agrobacterium-mediated transformation of flax (Linum usitatissimum)
    Frédéric Lamblin
    Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, Antenne Scientifique Universitaire de Chartres, Chartres, France
    Plant Cell Rep 26:765-72. 2007
    ..These results indicate that the PMI/mannose selection system can be successfully used for the recovery of flax transgenic plants under safe conditions for human health and the environment...
  45. pmc Expression cloning and biochemical characterization of a Rhizobium leguminosarum lipid A 1-phosphatase
    Mark J Karbarz
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 278:39269-79. 2003
    Lipid A of Rhizobium leguminosarum, a nitrogen-fixing plant endosymbiont, displays several significant structural differences when compared with Escherichia coli. An especially striking feature of R...
  46. ncbi The cin quorum sensing locus of Rhizobium etli CNPAF512 affects growth and symbiotic nitrogen fixation
    Ruth Daniels
    Centre of Microbial and Plant Genetics, Katholicke Universitat Leuven, Kasteelpark Arenberg 20, B 3001 Heverlee, Belgium
    J Biol Chem 277:462-8. 2002
    b>Rhizobium etli CNPAF512 produces an autoinducer that inhibits growth of Rhizobium leguminosarum bv. viciae 248 and activates the Agrobacterium tumefaciens tra reporter system. Production of this compound in R...
  47. ncbi Host-specific regulation of symbiotic nitrogen fixation in Rhizobium leguminosarum biovar trifolii
    Simon H Miller
    Department of Microbiology and Immunology, University of Otago, PO Box 56, Dunedin, New Zealand
    Microbiology 153:3184-95. 2007
    Strains of Rhizobium leguminosarum bv...
  48. pmc Identification and sequence analysis of the Rhizobium meliloti dctA gene encoding the C4-dicarboxylate carrier
    T Engelke
    Lehrstuhl fur Genetik, Fakultat fur Biologie, Universitat Bielefeld, Federal Republic of Germany
    J Bacteriol 171:5551-60. 1989
    Transposon Tn5-induced C4-dicarboxylate transport mutants of Rhizobium meliloti 2011 which could be complemented by cosmid pRmSC121 were subdivided into two classes...
  49. pmc Characterization of a {gamma}-aminobutyric acid transport system of Rhizobium leguminosarum bv. viciae 3841
    J P White
    University of Reading, United Kingdom
    J Bacteriol 191:1547-55. 2009
    Spontaneous mutants of Rhizobium leguminosarum bv. viciae 3841 were isolated that grow faster than the wild type on gamma-aminobutyric acid (GABA) as the sole carbon and nitrogen source...
  50. ncbi Evolution of nitrogen fixation in spatially structured populations of Rhizobium
    J D Bever
    Department of Biology, Indiana University, Bloomington, IN 47405, USA
    Heredity (Edinb) 85:366-72. 2000
    ..plant exudates and nitrogen-fixing populations are therefore vulnerable to invasion by nonfixing, saprophytic Rhizobium. The access of nonfixing Rhizobium to the plant exudates associated with nodules depends upon the spatial ..
  51. ncbi A gene cluster encoding malonyl-CoA decarboxylase (MatA), malonyl-CoA synthetase (MatB) and a putative dicarboxylate carrier protein (MatC) in Rhizobium trifolii--cloning, sequencing, and expression of the enzymes in Escherichia coli
    J H An
    Department of Biochemistry, College of Science, Bioproducts Research Center, Yonsei University, Seoul, Korea
    Eur J Biochem 257:395-402. 1998
    ..of three consecutive genes, matABC, was isolated using a probe prepared from amino acid sequence information of Rhizobium trifolii malonyl-CoA synthetase, and was subsequently sequenced...
  52. ncbi Cloning and overexpression of glycosyltransferases that generate the lipopolysaccharide core of Rhizobium leguminosarum
    J L Kadrmas
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 273:26432-40. 1998
    The lipopolysaccharide (LPS) core of the Gram-negative bacterium Rhizobium leguminosarum is more amenable to enzymatic study than that of Escherichia coli because much of it is synthesized from readily available sugar nucleotides...
  53. doi Rhizobium multihospitium sp. nov., isolated from multiple legume species native of Xinjiang, China
    Tian Xu Han
    Key Laboratory of Agro Microbial Resource and Application, Ministry of Agriculture College of Biological Sciences, China Agricultural University, Beijing 100094, PR China
    Int J Syst Evol Microbiol 58:1693-9. 2008
    ..These strains were classified as belonging to the genus Rhizobium based on amplified 16S rDNA restriction analysis (ARDRA)...
  54. pmc Establishing molecular tools for genetic manipulation of the pleuromutilin-producing fungus Clitopilus passeckerianus
    Sreedhar Kilaru
    School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, United Kingdom
    Appl Environ Microbiol 75:7196-204. 2009
    ..passeckerianus. The molecular tools developed in this study should facilitate studies aimed at gene isolation or characterization in this pharmaceutically important species...
  55. pmc The CcrM DNA methyltransferase is widespread in the alpha subdivision of proteobacteria, and its essential functions are conserved in Rhizobium meliloti and Caulobacter crescentus
    R Wright
    Department of Developmental Biology, Stanford University, California 94305 5427, USA
    J Bacteriol 179:5869-77. 1997
    ..We have amplified and sequenced a 258-bp region of the cerM gene from several of these bacteria, including Rhizobium meliloti, Brucella abortus, Agrobacterium tumefaciens, and Rhodobacter capsulatus...
  56. ncbi Antibacterial activity of Cuminum cyminum L. and Carum carvi L. essential oils
    Nicola S Iacobellis
    Dipartimento di Biologia, Difesa e Biotecnologie Agro Forestali, Universita degli Studi della Basilicata, Viale dell Ateneo Lucano 10, 85100 Potenza, Italy
    J Agric Food Chem 53:57-61. 2005
    ..In general, a lower activity was observed against bacteria belonging to the genus Pseudomonas. These results suggest the potential use of the above essential oils for the control of bacterial diseases...
  57. doi Synthesis and biological evaluation of novel biotin-iminoalditol conjugates
    Gerit Pototschnig
    Glycogroup, Institut fur Organische Chemie, Technische Universitat Graz, Stremayrgasse 16, A 8010 Graz, Austria
    Bioorg Med Chem Lett 20:4077-9. 2010
    ..with K(i) values in the range of the respective parent compounds. Such molecular probes have potential for activity-based protein profiling taking advantage of the biotin-(strept)avidin interaction...
  58. pmc Auxin and nitric oxide control indeterminate nodule formation
    Youry Pii
    Dipartimento Scientifico Tecnologico, University of Verona, Verona, Italy
    BMC Plant Biol 7:21. 2007
    ..Both plants and rhizobia synthesise auxin. We have addressed the effects of bacterial auxin (IAA) on nodulation by using Sinorhizobium meliloti and Rhizobium leguminosarum bacteria genetically engineered for increased auxin synthesis.
  59. doi Oral immunogenicity of porcine reproductive and respiratory syndrome virus antigen expressed in transgenic banana
    Hui Ting Chan
    Department of Horticulture and Landscape Architecture, National Taiwan University, Taiwan, Republic of China
    Plant Biotechnol J 11:315-24. 2013
    ....
  60. ncbi Toxic effects of arsenic on Sinorhizobium-Medicago sativa symbiotic interaction
    Eloisa Pajuelo
    Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain
    Environ Pollut 154:203-11. 2008
    Recently, the Rhizobium-legume symbiotic interaction has been proposed as an interesting tool in bioremediation. However, little is known about the effect of most common contaminants on this process...
  61. ncbi Identification of organelles in bacteria similar to acidocalcisomes of unicellular eukaryotes
    Manfredo Seufferheld
    Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana Champaign, Urbana, Illinois 61802, USA
    J Biol Chem 278:29971-8. 2003
    ..The results suggest that acidocalcisomes arose before the prokaryotic and eukaryotic lineages diverged...
  62. ncbi Allelic relationships of pea nodulation mutants
    K Novak
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    J Hered 94:191-3. 2003
    ..were evaluated for the symbiotic phenotype under controlled growth conditions against the nodule bacterium Rhizobium leguminosarum bv. viciae strain 248...
  63. pmc Binding site determinants for the LysR-type transcriptional regulator PcaQ in the legume endosymbiont Sinorhizobium meliloti
    Allyson M MacLean
    Center for Environmental Genomics, Department of Biology, McMaster University, Hamilton, Ontario, Canada
    J Bacteriol 190:1237-46. 2008
    ..this work describes the first reported purification of this regulator, as well as characterization of its binding site, which is conserved in Agrobacterium tumefaciens, Rhizobium leguminosarum, Rhizobium etli, and Mesorhizobium loti.
  64. doi pBINPLUS/ARS: an improved plant transformation vector based on pBINPLUS
    William Belknap
    USDA Agricultural Research Service, Albany, CA 94710 1105, USA
    Biotechniques 44:753-6. 2008
    ..pBINPLUS/ARS has been used to introduce transgenes into potato and other crop species and is available to all researchers in academic, government, and industrial laboratories for proof-of-principle and commercial applications...
  65. doi Genome engineering of Agrobacterium tumefaciens using the lambda Red recombination system
    Shengbiao Hu
    College of Life Science, Hunan Normal University, Hunan Provincial Key Laboratory of Microbial Molecular Biology, State Key Laboratory Breeding Base of Microbial Molecular Biology, Changsha, People s Republic of China
    Appl Microbiol Biotechnol 98:2165-72. 2014
    ..These results extend recombineering technology to a new host and indicate a fast and convenient way to engineer the A. tumefaciens genome for functional genomics and strain improvements...
  66. doi Morphology and general characteristics of bacteriophages infectious to Robinia pseudoacacia mesorhizobia
    Anna Turska-Szewczuk
    Department of Genetics and Microbiology, Maria Curie Skłodowska University, 19 Akademicka St, 20 033 Lublin, Poland
    Curr Microbiol 61:315-21. 2010
    ..in respect of their virulence, as they only lysed Mesorhizobium strains, but did not affect strains of Rhizobium or Bradyrhizobium...
  67. doi Genetic diversity of root-nodulating bacteria isolated from pea (Pisum sativum) in subtropical regions of China
    Chengyun Yang
    State Key Laboratory of Agro Microbiology, Huazhong Agricultural University, Wuhan, 430070, China
    Sci China C Life Sci 51:854-62. 2008
    ..5%) to Rhizobium leguminosarum USDA 2370. Group II consisting of WzP3 and WzP15 was closely related to Rhizobium etli CFN42...
  68. ncbi Role of rhizobial biosynthetic pathways of amino acids, nucleotide bases and vitamins in symbiosis
    Gursham S Randhawa
    Department of Biosciences and Biotechnology, Indian Institute of Technology, Roorkee 247 667, India
    Indian J Exp Biol 40:755-64. 2002
    ..Some of these amino acids are synthesized by rhizobium, whereas the remaining are supplied by the host plant. The supply from plant appears to be plant-type specific...
  69. pmc GS52 ecto-apyrase plays a critical role during soybean nodulation
    Manjula Govindarajulu
    Donald Danforth Plant Science Center, St Louis, Missouri 63132, USA
    Plant Physiol 149:994-1004. 2009
    ..These results clearly suggest that GS52 ecto-apyrase catalytic activity is critical for the early B. japonicum infection process, initiation of nodule primordium development, and subsequent nodule organogenesis in soybean...
  70. pmc OryR is a LuxR-family protein involved in interkingdom signaling between pathogenic Xanthomonas oryzae pv. oryzae and rice
    Sara Ferluga
    Bacteriology Group, International Center for Genetic Engineering and Biotechnology, Padriciano 99, 34012 Trieste, Italy
    J Bacteriol 191:890-7. 2009
    ..Therefore, OryR has unique features and is an important regulator involved in interkingdom communication between the host and the pathogen...
  71. doi MALDI-TOF characterization of hGH1 produced by hairy root cultures of Brassica oleracea var. italica grown in an airlift with mesh bioreactor
    Edgar García López
    Biotecnología y Bioingeniería, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, Avenida Instituto Politecnico Nacional 2508, Colonia San Pedro Zacatenco, Mexico Distrito Federal, CP, 07360
    Biotechnol Prog 30:161-71. 2014
    ..These results demonstrate that hairy root cultures of broccoli have potential as an alternative expression system for production of hGH1, and might also be useful for production of other therapeutic proteins...
  72. ncbi Production of herbicide-resistant transgenic Panax ginseng through the introduction of the phosphinothricin acetyl transferase gene and successful soil transfer
    Y E Choi
    Korea Ginseng Institute, Chung Ang University, Ansung, 456 756 Kyunggi do, South Korea
    Plant Cell Rep 21:563-8. 2003
    ..Transgenic plants growing in soil were observed to be strongly resistant to Basta application...
  73. ncbi Efficient transformation of Medicago truncatula cv. Jemalong using the hypervirulent Agrobacterium tumefaciens strain AGL1
    M Chabaud
    Laboratoire des Interactions Plantes Microorganismes, INRA CNRS, UMR215, BP 27, 31326 Castanet Tolosan Cedex, France
    Plant Cell Rep 22:46-51. 2003
    ..Transgene expression in planta was analysed and found to be conserved in the T(1) descendents...
  74. pmc Probing the catalytic roles of Arg548 and Gln552 in the carboxyl transferase domain of the Rhizobium etli pyruvate carboxylase by site-directed mutagenesis
    Saowapa Duangpan
    Molecular Metabolism Research Group, Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
    Biochemistry 49:3296-304. 2010
    The roles of Arg548 and Gln552 residues in the active site of the carboxyl transferase domain of Rhizobium etli pyruvate carboxylase were investigated using site-directed mutagenesis...
  75. doi Tsw gene-based resistance is triggered by a functional RNA silencing suppressor protein of the Tomato spotted wilt virus
    Dryas de Ronde
    Laboratory of Virology, Department of Plant Sciences, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
    Mol Plant Pathol 14:405-15. 2013
    ..The role of NSs as RNA silencing suppressor and Avr determinant is discussed in the light of a putative interplay between RNAi and the natural Tsw resistance gene...
  76. pmc Manipulation of auxin transport in plant roots during Rhizobium symbiosis and nematode parasitism
    Wim Grunewald
    Department Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, B 9000 Gent, Belgium
    Plant Cell 21:2553-62. 2009
    ..In this review, we focus on recent findings in auxin transport mechanisms during plant development and on how plant symbionts and parasites have evolved to manipulate these mechanisms for their own purposes...
  77. pmc The non-specific lipid transfer protein N5 of Medicago truncatula is implicated in epidermal stages of rhizobium-host interaction
    Youry Pii
    Department of Biotechnology, University of Verona, Strada Le Grazie 15, Verona, 37134, Italy
    BMC Plant Biol 12:233. 2012
    ....
  78. ncbi Isoflavonoid accumulation in soybean hairy roots upon treatment with Fusarium solani
    Vera V Lozovaya
    Department of Crop Sciences, University of Illinois, 1201 W Gregory Drive, Urbana, IL 61801, USA
    Plant Physiol Biochem 42:671-9. 2004
    ..The data obtained indicate that the ability of soybean roots to rapidly produce sufficient amounts of glyceollin in response to FSG infection might be important in providing partial resistance to this fungus...
  79. doi The grape microvine - a model system for rapid forward and reverse genetics of grapevines
    Jamila Chaïb
    CSIRO Plant Industry, Adelaide Laboratory, Glen Osmond, SA 5064, Australia
    Plant J 62:1083-92. 2010
    ..The microvine provides a unique model system for rapid genetic studies of grapevine by changing the perennial long life cycle of the plant to one with features and advantages similar to an annual plant...
  80. ncbi Over-expression of SOB5 suggests the involvement of a novel plant protein in cytokinin-mediated development
    Jingyu Zhang
    Department of Biology, Washington University, Campus Box 1137, One Brookings Drive, St Louis, MO 63130, USA
    Plant J 46:834-48. 2006
    ..Taken together, our data suggest a role for the novel gene SOB5 in cytokinin-mediated plant development...
  81. doi Horizontal gene transfer from genus agrobacterium to the plant linaria in nature
    Tatiana V Matveeva
    Department of Genetics, St Petersburg State University, St Petersburg, Russia
    Mol Plant Microbe Interact 25:1542-51. 2012
    ..Attempts to demonstrate expression of the T-DNA genes were negative. Our study indicates that the frequency of gene transfer and fixation in the germline from Agrobacterium to plant hosts is rare in the natural environment...
  82. ncbi [Identification of the regulation elements in heat-inducible Lehsp23.8 promoter]
    Shuying Yi
    Department of Biochemistry, Taishan Medical College, Tai an 271000, China
    Sheng Wu Gong Cheng Xue Bao 25:826-31. 2009
    ..It also indicates that the sequences between -255 bp and -565 bp serve as enhancers, while the sequences between -565 bp and -871 bp can repress the heat-induced activity of the Lehsp23.8 promoter...
  83. doi Occurrence of polyamines in root nodules of Phaseolus vulgaris in symbiosis with Rhizobium tropici in response to salt stress
    Miguel Lopez-Gomez
    Departamento de Fisiologia Vegetal, Facultad de Ciencias, Universidad de Granada, Campus de Fuentenueva s n, 18071 Granada, Spain Electronic address
    Phytochemistry 107:32-41. 2014
    ..the involvement of PAs in the response to salt stress in root nodules of Phaseolus vulgaris in symbiosis with Rhizobium tropici...
  84. ncbi Agrobacterium-mediated transformation of yellow lupin to generate callus tissue producing HBV surface antigen in a long-term culture
    Tomasz Pniewski
    Institute of Plant Genetics, Polish Academy of Sciences, Poznan, Poland
    J Appl Genet 47:309-18. 2006
    ..Lupin tissue lines were long-term cultured on selection media maintaining the growth rate and high expression level of the native form of S-HBs, up to 6 microg per g of fresh tissue...
  85. doi Overexpression of osmotin gene confers tolerance to salt and drought stresses in transgenic tomato (Solanum lycopersicum L.)
    D Goel
    National Research Center on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, 110 012, India
    Protoplasma 245:133-41. 2010
    ..The present investigation clearly shows that overexpression of osmotin gene enhances salt and drought stress tolerance in transgenic tomato plants...
  86. doi Structural analysis of the O-polysaccharide from the lipopolysaccharide of Azospirillum brasilense S17
    Yuliya P Fedonenko
    Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Prospekt Entuziastov 13, 410049 Saratov, Russia
    Carbohydr Res 343:810-6. 2008
    ..degradation of the lipopolysaccharide isolated by the phenol/water extraction from the asymbiotic diazotrophic rhizobacterium Azospirillum brasilense S17...
  87. doi Light intensity and temperature affect systemic spread of silencing signal in transient agroinfiltration studies
    Basavaprabhu L Patil
    Donald Danforth Plant Science Center, 975 N Warson Rd, St Louis, MO, 63132, USA National Research Centre on Plant Biotechnology, IARI, Pusa Campus, New Delhi, 110012, India
    Mol Plant Pathol 16:484-94. 2015
    ....
  88. doi Production of acylated homoserine lactone by gram-positive bacteria isolated from marine water
    Pramal Biswa
    Bioengineering and Drug Design Lab, Department of Biotechnology, Indian Institute of Technology IIT Madras, Chennai, Tamil Nadu, India
    FEMS Microbiol Lett 343:34-41. 2013
    ..qPCR analysis suggests an increment in exgR mRNA on addition of AHL, further proving the role of ExgR as a QS regulator...
  89. ncbi Agrobacterium type IV secretion is a two-step process in which export substrates associate with the virulence protein VirJ in the periplasm
    Mario Pantoja
    Deparetment of Microbiology, University of Washington, Seattle, WA 98195, USA
    Mol Microbiol 45:1325-35. 2002
    ..Our analyses of protein-protein interactions in a variety of mutant backgrounds indicate that substrates are probably secreted independently of one another...
  90. doi Rhizobium etli taxonomy revised with novel genomic data and analyses
    Martha G López-Guerrero
    Centro de Ciencias Genomicas, UNAM, Cuernavaca, Morelos, Mexico
    Syst Appl Microbiol 35:353-8. 2012
    ..analyses with concatenated conserved genomic fragments accounting for over half of each genome showed that Rhizobium strains CIAT 652, Ch24-10 (newly reported genome) and CNPAF 512 constituted a well-supported group independent ..
  91. doi Colutea arborescens is nodulated by diverse rhizobia in Eastern Morocco
    Mohammed Ourarhi
    Laboratoire de Biologie des Plantes et des Microorganismes, Faculty of Sciences, Mohamed Premier University, Oujda, Morocco
    Arch Microbiol 193:115-24. 2011
    ..A large diversity was observed among these rhizobia, and they were classified as different species of the genera Rhizobium, Sinorhizobium and Mesorhizobium...
  92. pmc The effect of the Agrobacterium tumefaciens attR mutation on attachment and root colonization differs between legumes and other dicots
    A G Matthysse
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    Appl Environ Microbiol 67:1070-5. 2001
    ..The second system is AttR independent, is not inhibited by the acetylated capsular polysaccharide, and allows the bacteria to bind to the roots of legumes...
  93. ncbi Phylogenetic diversity of rhizobial strains nodulating Robinia pseudoacacia L
    A Ulrich
    ZALF Centre for Agricultural Landscape and Land Use Research, Institute of Primary Production and Microbial Ecology, Eberswalder Str 84, D 15374 Müncheberg, Germany
    Microbiology 146:2997-3005. 2000
    ..Based on RFLP analysis and sequencing, the strains were shown to belong to the genera Mesorhizobium (76%) and Rhizobium (24%)...
  94. pmc Isolation and functional gene analyses of aromatic-hydrocarbon-degrading bacteria from a polychlorinated-dioxin-dechlorinating process
    Shinichi Kaiya
    Department of Environmental and Life Sciences, Toyohashi University of Technology, Toyohashi 441 8580, Japan
    Microbes Environ 27:127-35. 2012
    ..of the phyla Actinobacteria, Firmicutes, and Proteobacteria, among which those of the genera Paenibacillus and Rhizobium were most abundant...
  95. doi Genetic transformation using maize immature zygotic embryos
    Bronwyn Frame
    Plant Science Institute, Department of Agronomy, Center for Plant Transformation, Iowa State University, Ames, IA, USA
    Methods Mol Biol 710:327-41. 2011
    ..Two different media regimes are used for successful culture and transformation of two distinct genotypes...
  96. doi The Structural Features of Thousands of T-DNA Insertion Sites Are Consistent with a Double-Strand Break Repair-Based Insertion Mechanism
    Nils Kleinboelting
    Center for Biotechnology and Department of Biology, Bielefeld University, Universitaetsstrasse 25, 33615 Bielefeld, Germany
    Mol Plant 8:1651-64. 2015
    ..In addition, we describe unexpected integration events, such as large deletions and inversions at the integration site that are relevant for correct interpretation of results from T-DNA insertion mutants in reverse genetics experiments...
  97. doi Highly efficient Agrobacterium-mediated transformation of banana cv. Rasthali (AAB) via sonication and vacuum infiltration
    Kondeti Subramanyam
    Department of Biochemistry, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh 515055, India
    Plant Cell Rep 30:425-36. 2011
    ....
  98. pmc Linear chromosome-generating system of Agrobacterium tumefaciens C58: protelomerase generates and protects hairpin ends
    Wai Mun Huang
    Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, Utah 84112, USA
    J Biol Chem 287:25551-63. 2012
    ..The biochemical analyses of the protelomerase reactions further revealed that the tip of the hairpin telomere may be unusually polymorphically capable of accommodating any nucleotide...
  99. pmc A Genetic Screen for Pathogenicity Genes in the Hemibiotrophic Fungus Colletotrichum higginsianum Identifies the Plasma Membrane Proton Pump Pma2 Required for Host Penetration
    MARTIN KORN
    Department of Biology, Division of Biochemistry, Friedrich Alexander University Erlangen Nuremberg, Staudtstrasse 5, 91058 Erlangen, Germany
    PLoS ONE 10:e0125960. 2015
    ..We propose that expression of a specific proton pump early during infection may be common to many appressoria forming fungal pathogens as we found ChPMA2 orthologs in several plant pathogenic fungi...
  100. doi Characterization of a chalcone synthase (CHS) flower-specific promoter from Lilium orential 'Sorbonne'
    Yali Liu
    College of Forestry, Northwest A and F University, Yangling, 712100, Shaanxi, People s Republic of China
    Plant Cell Rep 30:2187-94. 2011
    ..This evidence combined with prediction of cis-acting elements in the PLoCHS promoter suggests that the TACPyAT box located in this promoter plays a key role in the regulation of organ-specific expression...
  101. pmc Differential and independent roles of a sigma(32) homolog (RpoH) and an HrcA repressor in the heat shock response of Agrobacterium tumefaciens
    K Nakahigashi
    HSP Research Institute, Kyoto Research Park, Shimogyo ku, Kyoto 600 8813, Japan
    J Bacteriol 181:7509-15. 1999
    ..We conclude that RpoH plays a major and global role in the induction of most heat shock proteins, whereas HrcA plays a restricted role in repressing groE expression under nonstress conditions...

Research Grants76

  1. OXYGEN REGULATION OF SYMBIOTIC GENES IN RHIZOBIUM
    Gary Ditta; Fiscal Year: 1993
    The long term goal of our research is to try and understand how the bacterium Rhizobium meliloti establishes a nitrogen-fixing symbiosis with its legume host alfalfa...
  2. TAXIS OF RHIZOBIA TO LOCALIZED SITES ON LEGUME ROOTS
    KOSTIA BERGMAN; Fiscal Year: 1990
    ..This assay is made specific by the use of two mutants of Rhizobium meliloti strain SU47 that no longer detect any pure substance tested (primarily sugar and amino acids) but still ..
  3. Arrested Development of Arabidopsis
    F Pickett; Fiscal Year: 2003
    ..These projects will support our long-term goal to understand the informational context required for meristem initiation and function, and to understand the regulatory interplay occurring between the meristem and developing leaves. ..
  4. MOLECULAR GENETICS OF RHIZOBIUM NODULATION PLASMIDS
    Graham C Walker; Fiscal Year: 2013
    ..The project has offered novel insights into the biosynthesis of B12, a critically important vitamin for human health, and will continue to explore the role of vitamin B12 in biology. ..
  5. Genetics and biosynthesis of an O antigen essential for symbiosis
    KENNETH D NOEL; Fiscal Year: 2010
    ..There are two well-differentiated mechanisms of O-antigen synthesis. The LPS of Rhizobium etli CE3 is one of the better characterized examples (structurally and biologically) in which the O-antigen ..
  6. REGULATION OF NODULATION GENES IN RHIZOBIUM MELILOTI
    Sharon Long; Fiscal Year: 2002
    Our proposal concerns the regulation of gene expression in a prokaryote, Rhizobium meliloti, that infects and establishes symbiosis with eukaryotic plant hosts such as alfalfa (Medicago sativa)...
  7. TRIFOLIIN A & ITS RECEPTORS ON RHIZOBIUM TRIFOLII
    Frank Dazzo; Fiscal Year: 1990
    b>Rhizobium trifolii is a gram-negative bacterium which specifically infects clover root hairs and forms a root nodule symbiosis which fixes atmospheric nitrogen into ammonia...
  8. HOST RECOGNITION AND INFECTION OF SOYBEAN BY RHIZOBIUM
    Gary Stacey; Fiscal Year: 1990
    ..The outer membrane of B. japonicum appears to undergo modification early in the infection cycle. All of the above cell surface changes may be critical to the determination of host specificity...
  9. RHIZOBIUM EXOPOLYSACCHARIDES AND NODULATION
    JOHN LEIGH; Fiscal Year: 1992
    An integrated genetic and biochemical approach will be used to study Rhizobium meliloti extracellular polysaccharides, and the role of these polysaccharides in the formation of nitrogen fixing root nodules on alfalfa...
  10. TRANSLOCATION OF DNA ACROSS THE AGROBACTERIUM ENVELOPE
    Peter J Christie; Fiscal Year: 2013
    ..These systems represent novel targets for therapeutics directed to blocking antibiotic resistance spread and bacterial disease progression. ..
  11. Regulatory Circuits Controlling Development of Dormant Microbial Cysts
    CARL EUGENE BAUER; Fiscal Year: 2013
    ..and has implicated cGMP production in many other important bacterial species such as Azospirillum brasilense, Rhizobium leguminosarum, Mesorhizobium loti, Sinorhizobium medicae, Sinorhizobium meliloti, Sinorhizobium fredii, Delftia ..
  12. GORDON CONFERENCE ON MICROBIAL ADHESION MECHANISMS
    Roy Curtiss; Fiscal Year: 1993
    ..to the molecular signaling of events that occur in plant-microbe interactions leading to nitrogen fixation by Rhizobium species on leguminous plants...
  13. Control of bacterial morphology and differentiation
    Yves V Brun; Fiscal Year: 2013
    ..Insights gained from these studies can be used to design strategies to inhibit growth, prevent key morphological changes, or alter important protein localization pathways in pathogens, thereby improving our ability to control them. ..
  14. Interconnectivity between genome packagaing and other viral functions
    A L Rao; Fiscal Year: 2010
    ....
  15. DnaK Protein Inhibitor Antimicrobials
    Michael Sturgess; Fiscal Year: 2003
    ..abstract_text> ..
  16. Molecular Mechanisms that Regulate Phase Transition in Dimorphic Fungi
    Gregory M Gauthier; Fiscal Year: 2010
    ..The research proposed is relevant to public health because it will provide insight into the mechanisms by which fungi cause disease and may lead to the identification of new antifungal drug targets. ..
  17. Ecological dynamics of facultative bacterial pathogens
    JAMES DAVID BEVER; Fiscal Year: 2013
    ..These results then form the basis for evaluating the importance of horizontal gene transfer of virulence genes and for scaling up to infection dynamics and disease incidence. ..
  18. MOLECULAR ANALYSIS OF FLOWERING GENES OF PHARBITIS NIL
    Anath Das; Fiscal Year: 1991
    ..thaliana genes by similar procedures. Induction of flowering is a critical process in angiosperm lifecycle. Understanding how photoperiod control this process will greatly broaden our knowledge on developmental processes in plants...
  19. Developmental Asymmetry in Agrobacterium tumefaciens
    JASON EUGENE HEINDL; Fiscal Year: 2013
    ..tumefaciens will be identified using a combination of transposon mutagenesis and a novel high-throughput microscopy screen. ..
  20. Characterization of the helical localization pattern of the T4SS in Agrobacterium
    Julieta Aguilar; Fiscal Year: 2010
    ..This observation leads us to propose that the cytoskeleton of Agrobacteria has a role In the localization of the T4SS. In specific aim 2 will investigate the potential role of the cytoskeleton on T4SS assembly and function. ..
  21. MOLECULAR ANALYSIS OF A POTENTIAL TRANSPORT OPERON IN AA
    Karen Novak; Fiscal Year: 2001
    ..Identification of genes involved in the secretion of macromolecules from Aa may lead to the development of strategies aimed at diminishing the virulence potential of this pathogenic microorganism. ..
  22. Genetic analysis of Cryptococcus neoformans virulence
    Joseph Heitman; Fiscal Year: 2009
    ..These studies will enable a genome-wide definition of the gene set contributing to virulence of this common human fungal pathogen. ..
  23. A molecular toolbox for hypothesis testing in the dermatophytes
    Theodore C White; Fiscal Year: 2010
    ..This proposal will develop a set of molecular tools that can be used in combination with the soon-to-be-completed genome sequences to address important questions about dermatophyte prevention, treatment and diagnosis. ..
  24. REGULATION OF SYNTHESIS OF A HORMONE INDUCED PROTEIN
    GLENN GALAU; Fiscal Year: 1991
    ..These are predicted to be deficient in Lea function and as such should help elucidate both the regulation and function of these proteins...
  25. Modulation of two-component signaling in alphaproteobacteria
    Joel Griffitts; Fiscal Year: 2009
    ..Since this signaling system is also present in several animal pathogens, this project promises to yield broad molecular-level insights into host-microbe interactions. ..
  26. CARBOHYDRATE BINDING PROTEIN OF B. JAPONICUM
    SIUCHEONG HO; Fiscal Year: 1993
    ..This specificity correlated with the behavior of intact Rhizobium in four carbohydrate-specific binding assays: (a) adsorption to Lac-Sepharose beads; (b) heterotypic binding to ..
  27. TAXOL PRODUCTION VIA IN VITRO CULTURE TECHNIQUE
    CHING YEH HU; Fiscal Year: 1992
    ....
  28. Intracellular screens discovery of natural products*(RMI)
    Jo Handelsman; Fiscal Year: 2007
    ..This research will lead to universal expression systems that will be useful for metagenomic analysis and other techniques that rely on heterologous gene expression. ..
  29. FLOW CYTOMETER FOR RESEARCH IN THE BIOLOGICAL SCIENCES
    Patricia Jones; Fiscal Year: 1999
    ..The fourth project will use GFP reporter constructs to identify developmentally-controlled promoters in Rhizobium bacteria solely expressed in symbiotic root nodules, perhaps induced by host signal molecules...
  30. tRNA mediated assembly in an icosahedral RNA virus
    A L Rao; Fiscal Year: 2006
    ..Collectively, the proposed studies will advance our understanding of the assembly mechanisms of icosahedral viruses that are known to cause economically important diseases in humans, animals, insects and plants. ..
  31. REGULATION OF GLYCOLYTIC GENES IN ARABIDOPSIS
    Ming Che Shih; Fiscal Year: 1993
    ....
  32. EXPRESSION OF SIVmac239 GP120 IN TRANSGENIC MAIZE
    JOSEPH JILKA; Fiscal Year: 2001
    ..This application will serve as a model for the exploration of the use of this methodology to develop HIV vaccines, potentially against a number of important HIV epitopes, for the control of HIV infection. ..
  33. Identification of Brucella Type IV Effectors
    RENEE TSOLIS; Fiscal Year: 2006
    ..It is our expectation that the results of this work will identify B. abortus Type IV effector proteins that will form the basis for a future R01 application to characterize their function. ..
  34. T-DNA INTEGRATION DURING CROWN GALL TUMORIGENESIS
    LLOYD REAM; Fiscal Year: 1991
    ..Thus, A. tumefaciens may serve as a model for rickettsial pathogenesis as well as human tumorigenesis...
  35. CIS & TRANS-ACTING COMPONENTS OF T-DNA PROMOTER FUNCTION
    WILLIAM GURLEY; Fiscal Year: 1992
    ..Although T-DNA genes may be bacterial in evolutionary origin, and certainly by lineage, they have achieved a close mimicry of plant genes from a functional standpoint and in their interactions with the trans-acting factors...
  36. Approach to control caries through apple-derived vaccine
    Hilary Koprowski; Fiscal Year: 2006
    ..The proposed research will initiate a novel approach to develop a plant fruit-based vaccine delivery system for a pharmaceutical vaccine against Dental caries and other infectious diseases. ..
  37. GENETICS OF HOST-SPECIFIC INFECTION BY RHIZOBIA
    Gary Stacey; Fiscal Year: 1990
    ..This proposal seeks to address this question using the soybean- rhizobium infection process as a model...
  38. Passive immunotherapy using plant-derived broadly HIV-1 neutralizing MAbs to prev
    Yvonne Rosenberg; Fiscal Year: 2009
    ..In this proposal, these MAbs will be produced in tobacco plants and their ability to neutralize HIV in vitro and to protect monkeys in vivo will be compared to currently used and expensive CHO-derived MAbs. ..
  39. A Plant-Derived, Multi-Component Tuberculosis Vaccine
    AMANDA WALMSLEY; Fiscal Year: 2004
    ..abstract_text> ..
  40. MECHANISM OF PLASMID DNA TRANSFER IN BACTERIA
    Donald Guiney; Fiscal Year: 1993
    ....
  41. Early Host Response Elicitation by Agrobacterium
    Marion Brodhagen; Fiscal Year: 2004
    ..abstract_text> ..
  42. REGULATION OF NODULATION GENES IN RHIZOBIUM MELILOTI
    Sharon Long; Fiscal Year: 1992
    ..We study the nutritionally mutualistic interaction or Rhizobium meliloti with a host legume, alfalfa...
  43. Effectors and Suppressors of RNA Silencing
    Lisa Johansen; Fiscal Year: 2004
    ..Introducing a combination of components and effectors of the silencing pathway will allow for a more detailed understanding of the initiation, maintenance, and signaling stages of RNA silencing. ..
  44. INTERACTIONS BETWEEN AGROBACTERIUM AND HOST PLANTS
    STEPHEN WINANS; Fiscal Year: 1993
    ..By analyzing their predicted amino acid sequence and by characterizing stains with mutations in each of these genes we will gain insights about their role in the maintenance of this parasitic association...
  45. TAXOL AND TAXANES FROM PLANT TISSUE CULTURE
    Ching Jer Chang; Fiscal Year: 1993
    ....
  46. CHEMICAL & GENETIC APPROACHES TO DEFINE CELL ACTIVATION
    David Lynn; Fiscal Year: 2005
    ..In addition, this specific signaling system regulates the only know natural vector for inter-Kingdom gene transfer and understanding this mechanism has already and will continue to widened the range of its use in biotechnology. ..
  47. GENOMICS APPROACH TO A PATHOGEN OF ANIMALS AND PLANTS
    BRADLEY GOODNER; Fiscal Year: 2000
    ..tumefaciens genome in order to search new genes involved in virulence or interaction, and 4) to functionally characterize a few of these new genes. Both biochemical and genetic approaches will be used to test gene functions. ..
  48. A DeltaVision Restoration Microscope forCNR Imaging
    Steven Ruzin; Fiscal Year: 2002
    ..abstract_text> ..
  49. RHIZOBIAL LIPOPOLYSACCHARIDES ESSENTIAL FOR INFECTION
    Russell W Carlson; Fiscal Year: 2010
    b>Rhizobium-legume symbioses involve processes that are fundamental to all bacterial-host interactions. These include (a.) prokaryotic-eukaryotic recognition, (b.) interaction with the host defense mechanism, and (c...
  50. Siderophore transport mechanism via membrane receptors
    Ranjan Chakraborty; Fiscal Year: 2004
    ..For the mutant proteins showing normal binding and defective transport, methods of purification and crystallization will be standardized. ..
  51. Transcriptional Profiles of Escherichia coli Biofilms
    Robert McLean; Fiscal Year: 2002
    ..Overall, this project will explain several fundamental aspects of E. coli biofilm growth and suggest possible targets for future antibiotic research. ..
  52. MOLECULAR BASIS OF CROWN GALL TUMORIGENESIS
    Eugene Nester; Fiscal Year: 2005
    ..The studies proposed in this application should open up new vistas and provide an increased understanding of the many levels of interaction of Agrobacterium with its variety of eukaryotic hosts. ..
  53. Ehrilchiacidal Mechanisms In Leukocytes
    Yasuko Rikihisa; Fiscal Year: 2009
    ..The results may point to a potential target for new treatment and prevention of HME and HGE. ..
  54. MOLECULAR PATHOGENESIS OF BLASTOMYCOSIS
    Bruce Klein; Fiscal Year: 2003
    ..Results from this work will yield important new information about a putative virulence factor, how it works, and how it's expression is regulated by the fungus and modulated by the host during infection. ..
  55. Gene Expression Levels Across Diverse Genomes
    Samuel Karlin; Fiscal Year: 2006
    ..A third major objective of our research will be to extend our codon usage methods for predicting gene expression levels to eukaryotic genomes, including yeast, D. melanogaster, C. elegans, and human. [unreadable] [unreadable]..
  56. Evolution of Estrogen Receptor-Ligand Interactions
    Jennifer Fox; Fiscal Year: 2006
    ..Such an experimental system will allow the rigorous testing of hypotheses about ER-ligand structure function relationships and the dynamics of receptor-ligand coevolution. ..
  57. Use of an Organophosphorus (OP) Hydrolase for Acute OP Poisoning
    Steven Bird; Fiscal Year: 2008
    ..Our central hypothesis is that OpdA is safe and improves survival after poisoning with parathion. ..
  58. The Transcriptome of Pathogenic Aspergillus Fumigatus
    William Nierman; Fiscal Year: 2003
    ..abstract_text> ..
  59. Comparison of Human Ehrlichiosis Agent Genomes
    Yasuko Rikihisa; Fiscal Year: 2010
    ..chaffeensis virulence determinants and their pathogenic mechanisms. The results may point to potential chemotherapy, chemopreventive and/or vaccine candidates for treatment and prevention of human ehrlichiosis. ..
  60. Regulation of Burkholderia mallei virulence genes
    William Nierman; Fiscal Year: 2005
    ..It will provide critical information on virulence control and virulence mechanisms of B. mallei and will be crucial to the development of effective therapeutic interventions and vaccine strategies. ..
  61. MOLECULAR SEQUENCE DATA
    Samuel Karlin; Fiscal Year: 2001
    ..by spacings between repeats, and by properties of repeat families (intergenic, coding, direct, inverted, mixed). ..
  62. Novel accelerated in vitro breeding for phytoremediation
    MIHALY CZAKO; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  63. INTERACTIONS BETWEEN AGROBACTERIUM AND HOST PLANTS
    Stephen C Winans; Fiscal Year: 2010
    ..Direct targets of YenR and YenS will be identified and assessed for their roles in the biology and ecology of Y. enterocolitica and Y. pestis. ..
  64. GENE EXPRESSION BY HUMAN GRANULOCYTIC EHRLICHIOSIS AG
    Yasuko Rikihisa; Fiscal Year: 2004
    ..These efforts are expected to reveal an important role for the p44-multigene family in strategies of adaptive surface variation and avoidance of host immune responses. ..
  65. CELL-CELL SIGNALING IN MICROBE-HOST INTERACTIONS
    Stephen Farrand; Fiscal Year: 2007
    ..In the third goal we propose to explore the evolutionary diversity of the Ti plasmid quorum-sensing systems by isolating and characterizing plasmids that induce transfer genes in response to novel plant signals. ..
  66. Microarray Expression Analysis of Burkholderia Mallei
    William Nierman; Fiscal Year: 2003
    ..Function and virulence validation of the identified virulence genes will be explored by construction and phenotype analysis of targeted knockout strains. ..
  67. fMRI Assessment of Organophosphate Neurotoxicity
    Steven Bird; Fiscal Year: 2008
    ..Bird with the facilities, equipment and expertise necessary to achieve the goals set forth in this proposal. ..
  68. ROLES OF EHRLICHIAL OUTER MEMBRANE PROTEIN IN PARASITISM
    Yasuko Rikihisa; Fiscal Year: 2001
    ..Aim 5 addresses hypothesis 3 by examining the effects of immunization with the ehrlichial OMP in a mouse model of human ehrlichiosis. ..
  69. Host-Pathogen Interactions in Invasive Aspergillosis
    WILLIAM CHARLES NIERMAN; Fiscal Year: 2010
    ..necessary to maintain protective immunity against the invading fungus. This hithertn inaccessible ... in order to >.E =.m m'- N'3 ..' 'C3 _.n '-' a.. m._ o'< m- ..