Agrobacterium tumefaciens str. C58


Alias: Agrobacterium tumefaciens (strain C58 / ATCC 33970), Agrobacterium tumefaciens (strain C58), Agrobacterium tumefaciens str. C58 (Cereon), Agrobacterium tumefaciens str. C58 (Dupont), Agrobacterium tumefaciens str. C58 (U. Washington), Rhizobium radiobacter str. C58 (Cereon), Rhizobium radiobacter str. C58 (Dupont), Rhizobium radiobacter str. C58 (U. Washington)

Top Publications

  1. Matthysse A, Yarnall H, Boles S, McMahan S. A region of the Agrobacterium tumefaciens chromosome containing genes required for virulence and attachment to host cells. Biochim Biophys Acta. 2000;1490:208-12 pubmed
    ..The presence of such a large number of genes required for attachment to host cells suggests that the ability of A. tumefaciens to bind to plant cells may play an important role in the life of these bacteria. ..
  2. Li L, Jia Y, Hou Q, Charles T, Nester E, Pan S. A global pH sensor: Agrobacterium sensor protein ChvG regulates acid-inducible genes on its two chromosomes and Ti plasmid. Proc Natl Acad Sci U S A. 2002;99:12369-74 pubmed
    ..Sensing acidity appears to enable Agrobacterium to coordinate its coping with the environment of wounded plants to cause tumors. ..
  3. Shirasu K, Kado C. Membrane location of the Ti plasmid VirB proteins involved in the biosynthesis of a pilin-like conjugative structure on Agrobacterium tumefaciens. FEMS Microbiol Lett. 1993;111:287-94 pubmed
  4. Charles T, Nester E. A chromosomally encoded two-component sensory transduction system is required for virulence of Agrobacterium tumefaciens. J Bacteriol. 1993;175:6614-25 pubmed
    ..Site-specific insertion mutations in either chvG or chvI abolished tumor formation ability, as well as the ability to grow on complex media. Neither the genes which are regulated nor the inducing signal is known yet for this system. ..
  5. Li P, Everhart D, Farrand S. Genetic and sequence analysis of the pTiC58 trb locus, encoding a mating-pair formation system related to members of the type IV secretion family. J Bacteriol. 1998;180:6164-72 pubmed
    ..The products of several trb genes of the Ti plasmid are similar to those of other loci that encode DNA transfer or protein secretion systems, all of which are members of the type IV secretion family...
  6. Cascales E, Christie P. Definition of a bacterial type IV secretion pathway for a DNA substrate. Science. 2004;304:1170-3 pubmed
    ..Our results define the translocation pathway for a DNA substrate through a bacterial conjugation machine, specifying the contributions of each subunit of the secretory apparatus to substrate passage. ..
  7. Atmakuri K, Cascales E, Christie P. Energetic components VirD4, VirB11 and VirB4 mediate early DNA transfer reactions required for bacterial type IV secretion. Mol Microbiol. 2004;54:1199-211 pubmed
    ..Our findings define genetic requirements for DNA substrate binding and the early transfer reactions of a bacterial type IV translocation pathway. ..
  8. McCullen C, Binns A. Agrobacterium tumefaciens and plant cell interactions and activities required for interkingdom macromolecular transfer. Annu Rev Cell Dev Biol. 2006;22:101-27 pubmed
  9. Barbosa R, Benedetti C. BigR, a transcriptional repressor from plant-associated bacteria, regulates an operon implicated in biofilm growth. J Bacteriol. 2007;189:6185-94 pubmed publisher
    ..tumefaciens bigR mutant showed constitutive expression of operon genes and increased biofilm formation on glass surfaces and tobacco roots, indicating that the operon may play a role in cell adherence or biofilm development...

More Information

Publications155 found, 100 shown here

  1. Tomlinson A, Ramey Hartung B, Day T, Merritt P, Fuqua C. Agrobacterium tumefaciens ExoR represses succinoglycan biosynthesis and is required for biofilm formation and motility. Microbiology. 2010;156:2670-81 pubmed publisher
    ..meliloti. Mutations that suppress the exoR motility defect suggest a branched regulatory pathway controlling succinoglycan synthesis, motility and biofilm formation. ..
  2. Vi T, Nguyen T, Pham T, Nguyen H, Nguyen T, Tu Q, et al. Overexpression of the ZmDEF1 gene increases the resistance to weevil larvae in transgenic maize seeds. Mol Biol Rep. 2019;: pubmed publisher
    ..The recombinant ZmDEF1 protein inhibited the alpha-amylase activity of the maize weevil larvae, and its ability to inhibit alpha-amylase is 54.52-63.09% greater than the ZmDEF1 protein extracted from non-transgenic plants. ..
  3. Deeken R, Saupe S, Klinkenberg J, Riedel M, Leide J, Hedrich R, et al. The Nonspecific Lipid Transfer Protein AtLtpI-4 Is Involved in Suberin Formation of Arabidopsis thaliana Crown Galls. Plant Physiol. 2016;172:1911-1927 pubmed
    ..In crown galls, which endogenously express AtLtpI-4, it is involved in suberin formation. ..
  4. Wakarchuk W, Greenberg N, Kilburn D, Miller R, Warren R. Structure and transcription analysis of the gene encoding a cellobiase from Agrobacterium sp. strain ATCC 21400. J Bacteriol. 1988;170:301-7 pubmed
    ..A region of deduced protein sequence was homologous to a region from two other beta-glucosidase sequences. This region of homology contained a putative active site by analogy with the active site of hen egg white lysozyme. ..
  5. Marincs F, White D. Nopaline causes a conformational change in the NocR regulatory protein-nocR promoter complex of Agrobacterium tumefaciens Ti plasmid pTiT37. Mol Gen Genet. 1993;241:65-72 pubmed
    ..These results suggest that nopaline-induced alterations in the NocR protein-nocR promoter complex might control gene expression in the noc operon. ..
  6. Cho K, Fuqua C, Martin B, Winans S. Identification of Agrobacterium tumefaciens genes that direct the complete catabolism of octopine. J Bacteriol. 1996;178:1872-80 pubmed
    ..Insertions of the third group was localized to a gene whose product is required for degradation of proline. We therefore have identified all steps required for the catabolism of octopine to glutamate. ..
  7. Malgieri G, Russo L, Esposito S, Baglivo I, Zaccaro L, Pedone E, et al. The prokaryotic Cys2His2 zinc-finger adopts a novel fold as revealed by the NMR structure of Agrobacterium tumefaciens Ros DNA-binding domain. Proc Natl Acad Sci U S A. 2007;104:17341-6 pubmed publisher
    ..Mapping of the amino acids necessary for the DNA binding onto Ros87 structure reveals the protein surface involved in the DNA recognition mechanism of this new zinc-binding protein domain...
  8. Shen Y, Liu R, Wang H. Oxalate decarboxylase from Agrobacterium tumefaciens C58 is translocated by a twin arginine translocation system. J Microbiol Biotechnol. 2008;18:1245-51 pubmed
    ..tumefaciens C58. Further site-directed mutagenesis experiment demonstrated that the A. tumefaciens C58 OXDC is most likely translocated by a twin-arginine translocation (TAT) system. ..
  9. Haudecoeur E, Planamente S, Cirou A, Tanni res M, Shelp B, Mor ra S, et al. Proline antagonizes GABA-induced quenching of quorum-sensing in Agrobacterium tumefaciens. Proc Natl Acad Sci U S A. 2009;106:14587-92 pubmed publisher
  10. Segal G, Ron E. Heat shock transcription of the groESL operon of Agrobacterium tumefaciens may involve a hairpin-loop structure. J Bacteriol. 1993;175:3083-8 pubmed
    ..This conserved region is thought to form a hairpin-loop structure and may play a role in gene regulation during heat shock. ..
  11. Hennessy F, Boshoff A, Blatch G. Rational mutagenesis of a 40 kDa heat shock protein from Agrobacterium tumefaciens identifies amino acid residues critical to its in vivo function. Int J Biochem Cell Biol. 2005;37:177-91 pubmed publisher
  12. Su S, Stephens B, Alexandre G, Farrand S. Lon protease of the alpha-proteobacterium Agrobacterium tumefaciens is required for normal growth, cellular morphology and full virulence. Microbiology. 2006;152:1197-207 pubmed
    ..The lon mutant is highly attenuated for virulence, suggesting that Lon may be required for the proper expression, assembly or function of the VirB/D4-mediated T-DNA transfer system. ..
  13. Torres L, Salerno G. A metabolic pathway leading to mannosylfructose biosynthesis in Agrobacterium tumefaciens uncovers a family of mannosyltransferases. Proc Natl Acad Sci U S A. 2007;104:14318-23 pubmed
    ..Importantly, a protein phylogeny analysis indicated that mannosylfructose-phosphate synthase defines a unique group of mannosyltransferases. ..
  14. Ornelas A, Korczynska M, Ragumani S, Kumaran D, Narindoshvili T, Shoichet B, et al. Functional annotation and three-dimensional structure of an incorrectly annotated dihydroorotase from cog3964 in the amidohydrolase superfamily. Biochemistry. 2013;52:228-38 pubmed publisher
    ..3 × 10(4) M(-1) s(-1)), and ultimately acetyl-(R)-mandelate (k(cat)/K(m) = 2.8 × 10(5) M(-1) s(-1)). ..
  15. Chowdhury E, Higuchi K, Nagata S, Misono H. A novel NADP(+)-dependent serine dehydrogenase from Agrobacterium tumefaciens. Biosci Biotechnol Biochem. 1997;61:152-7 pubmed
    ..The Michaelis constants for L-serine, D-serine, and NADP+ were 42, 44, and 0.029 mM, respectively. ..
  16. Nakahigashi K, Ron E, Yanagi H, Yura T. Differential and independent roles of a sigma(32) homolog (RpoH) and an HrcA repressor in the heat shock response of Agrobacterium tumefaciens. J Bacteriol. 1999;181:7509-15 pubmed
    ..We conclude that RpoH plays a major and global role in the induction of most heat shock proteins, whereas HrcA plays a restricted role in repressing groE expression under nonstress conditions...
  17. Peng W, Nester E. Characterization of a putative RND-type efflux system in Agrobacterium tumefaciens. Gene. 2001;270:245-52 pubmed
    ..The possible role of the putative efflux pump coded by the ame genes is discussed...
  18. Ochiai A, Hashimoto W, Murata K. A biosystem for alginate metabolism in Agrobacterium tumefaciens strain C58: molecular identification of Atu3025 as an exotype family PL-15 alginate lyase. Res Microbiol. 2006;157:642-9 pubmed
    ..3 and 30 degrees C through a beta-elimination reaction, indicating that Atu3025 is an exotype alginate lyase potentially involved in the assimilation of LMW alginate in strain C58. ..
  19. Ochiai A, Yamasaki M, Mikami B, Hashimoto W, Murata K. Crystallization and preliminary X-ray analysis of an exotype alginate lyase Atu3025 from Agrobacterium tumefaciens strain C58, a member of polysaccharide lyase family 15. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006;62:486-8 pubmed
    ..Preliminary X-ray analysis showed that the Atu3025 crystal belonged to space group P2(1) and diffracted to 2.8 angstroms resolution, with unit-cell parameters a = 107.7, b = 108.3, c = 149.5 angstroms, beta = 91.5 degrees. ..
  20. Boshoff A, Stephens L, Blatch G. The Agrobacterium tumefaciens DnaK: ATPase cycle, oligomeric state and chaperone properties. Int J Biochem Cell Biol. 2008;40:804-12 pubmed
    ..Our research implies that the C-terminal alpha-helical subdomain is involved in higher order associations, while the substrate binding domain is possibly involved in dimerisation. ..
  21. Drolet M, Sasarman A. Cloning and nucleotide sequence of the hemA gene of Agrobacterium radiobacter. Mol Gen Genet. 1991;226:250-6 pubmed
    ..Primer extension experiments enabled us to identify two promoters for the hemA gene of A. radiobacter. One of these promoters shows some similarity to the first promoter of the hemA gene of R. meliloti. ..
  22. Segal G, Ron E. The groESL operon of Agrobacterium tumefaciens: evidence for heat shock-dependent mRNA cleavage. J Bacteriol. 1995;177:750-7 pubmed
    ..These findings indicate that the cleavage event is part of the heat shock regulation of the groESL operon in A. tumefaciens. ..
  23. Aly K, Baron C. The VirB5 protein localizes to the T-pilus tips in Agrobacterium tumefaciens. Microbiology. 2007;153:3766-75 pubmed publisher
    ..The data presented here suggest that VirB5 localizes at the T-pilus tip, and provide novel insights into its role during the type IV secretion process...
  24. von Lintig J, Zanker H, Schroder J. Positive regulators of opine-inducible promoters in the nopaline and octopine catabolism regions of Ti plasmids. Mol Plant Microbe Interact. 1991;4:370-8 pubmed
    ..These proteins are the only Ti plasmid functions necessary for the activation of the opine-induced promoters. We propose the names nocR and occR for the regulator genes in the noc and in the occ regions. ..
  25. Cooley M, D Souza M, Kado C. The virC and virD operons of the Agrobacterium Ti plasmid are regulated by the ros chromosomal gene: analysis of the cloned ros gene. J Bacteriol. 1991;173:2608-16 pubmed
    ..This suggests possible binding competition between Ros and VirG at the virC and virD promoters. That the Ros protein binds DNA is suggested by the presence of a 'zinc finger' consensus sequence in the protein. ..
  26. Hirooka T, Rogowsky P, Kado C. Characterization of the virE locus of Agrobacterium tumefaciens plasmid pTiC58. J Bacteriol. 1987;169:1529-36 pubmed
    ..tumefaciens and one which operates in Escherichia coli. virE is transcribed from left to right toward the T region. In A. tumefaciens, the expression of virE was induced by acetosyringone and required the presence of pTiC58...
  27. Zanker H, Lurz G, Langridge U, Langridge P, Kreusch D, Schroder J. Octopine and nopaline oxidases from Ti plasmids of Agrobacterium tumefaciens: molecular analysis, relationship, and functional characterization. J Bacteriol. 1994;176:4511-7 pubmed
    ..These experiments also showed that the formation of functional enzyme required close proximity of the subunit genes on the same plasmid and that even a reversal of the gene order (A-B instead of B-A) led to reduced activity. ..
  28. Ceci P, Ilari A, Falvo E, Chiancone E. The Dps protein of Agrobacterium tumefaciens does not bind to DNA but protects it toward oxidative cleavage: x-ray crystal structure, iron binding, and hydroxyl-radical scavenging properties. J Biol Chem. 2003;278:20319-26 pubmed publisher
    ..tumefaciens Dps may be envisaged to act in concert with catalase A to counteract the toxic effect of H2O2, the major component of the plant defense system when challenged by the bacterium...
  29. Elias Y, Huang R. Biochemical and structural studies of A-to-I editing by tRNA:A34 deaminases at the wobble position of transfer RNA. Biochemistry. 2005;44:12057-65 pubmed
    ..Furthermore, the structural study of prokaryotic tadA presented here also sheds light on tRNA substrate recognition and the possible evolutionary origin of the eukaryotic ADAT2-ADAT3 heterodimer. ..
  30. Ngok Ngam P, Ruangkiattikul N, Mahavihakanont A, Virgem S, Sukchawalit R, Mongkolsuk S. Roles of Agrobacterium tumefaciens RirA in iron regulation, oxidative stress response, and virulence. J Bacteriol. 2009;191:2083-90 pubmed publisher
    ..These data demonstrated that RirA is important for A. tumefaciens during plant-pathogen interactions...
  31. Tsai Y, Wang M, Gao C, Kl sener S, Baron C, Narberhaus F, et al. Small heat-shock protein HspL is induced by VirB protein(s) and promotes VirB/D4-mediated DNA transfer in Agrobacterium tumefaciens. Microbiology. 2009;155:3270-80 pubmed publisher
    ..In conclusion, increased HspL abundance is triggered in response to certain VirB protein(s) and plays a role in optimal VirB protein accumulation, VirB/D4-mediated DNA transfer and tumorigenesis...
  32. Dokpikul T, Chaoprasid P, Saninjuk K, Sirirakphaisarn S, Johnrod J, Nookabkaew S, et al. Regulation of the Cobalt/Nickel Efflux Operon dmeRF in Agrobacterium tumefaciens and a Link between the Iron-Sensing Regulator RirA and Cobalt/Nickel Resistance. Appl Environ Microbiol. 2016;82:4732-4742 pubmed publisher
    ..Cobalt and nickel cause DmeR to dissociate from the dmeRF promoter, which leads to expression of the metal efflux gene dmeF This work also revealed a connection between iron homeostasis and cobalt/nickel resistance in A. tumefaciens. ..
  33. Howell M, Aliashkevich A, Salisbury A, Cava F, Bowman G, Brown P. Absence of the Polar Organizing Protein PopZ Results in Reduced and Asymmetric Cell Division in Agrobacterium tumefaciens. J Bacteriol. 2017;199: pubmed publisher
    ..Finally, since many closely related plant and animal pathogens exhibit polar growth, discoveries in A. tumefaciens may be broadly applicable for devising antimicrobial strategies. ..
  34. Turk S, Nester E, Hooykaas P. The virA promoter is a host-range determinant in Agrobacterium tumefaciens. Mol Microbiol. 1993;7:719-24 pubmed
    ..These results indicate that the domains, acting as receptors for sugars and phenolic signals, must be conserved between the LHR and WHR VirA receptor proteins. ..
  35. Wright E, Deakin W, Shaw C. A chemotaxis cluster from Agrobacterium tumefaciens. Gene. 1998;220:83-9 pubmed
    ..Although the putative operon does not contain a cheW homologue, heterologous probing and PCR using consensus primers indicates that cheW maps elsewhere in the Agrobacterium genome. ..
  36. Luo Z, Farrand S. The Agrobacterium tumefaciens rnd homolog is required for TraR-mediated quorum-dependent activation of Ti plasmid tra gene expression. J Bacteriol. 2001;183:3919-30 pubmed
    ..Our data suggest that the defect in tra gene induction in the mutants results from lowered levels of TraR. In turn, production of sufficient amounts of TraR apparently is sensitive to a cellular function requiring RNase D. ..
  37. Chai Y, Winans S. RepB protein of an Agrobacterium tumefaciens Ti plasmid binds to two adjacent sites between repA and repB for plasmid partitioning and autorepression. Mol Microbiol. 2005;58:1114-29 pubmed publisher
    ..We propose that RepA and RepB form complexes that bind both sites, possibly causing a loop that is important for repression of the repABC operon. Binding at one or both sites may also be required for accurate plasmid partitioning...
  38. Wise A, Fang F, Lin Y, He F, Lynn D, Binns A. The receiver domain of hybrid histidine kinase VirA: an enhancing factor for vir gene expression in Agrobacterium tumefaciens. J Bacteriol. 2010;192:1534-42 pubmed publisher
    ..These phenotypes were corrected if the virG copy number was increased. In addition, we show that the VirA receiver domain can interact with the VirG DNA-binding domain...
  39. Pan Y, Wang Y, Fuqua C, Chen L. In vivo analysis of DNA binding and ligand interaction of BlcR, an IclR-type repressor from Agrobacterium tumefaciens. Microbiology. 2013;159:814-22 pubmed publisher
    ..Together, these in vivo results expand the biochemical findings and provide new mechanistic insights into BlcR-DNA interactions. ..
  40. Hagiya M, Close T, Tait R, Kado C. Identification of pTiC58 plasmid-encoded proteins for virulence in Agrobacterium tumefaciens. Proc Natl Acad Sci U S A. 1985;82:2669-73 pubmed
    ..The protein products of these genes are likely to function in some aspect of the host-range determination of A. tumefaciens. ..
  41. Shimoda N, Toyoda Yamamoto A, Aoki S, Machida Y. Genetic evidence for an interaction between the VirA sensor protein and the ChvE sugar-binding protein of Agrobacterium. J Biol Chem. 1993;268:26552-8 pubmed
    ..We also discuss the molecular architecture of the operon to which the chvE gene belongs. ..
  42. Lohrke S, Nechaev S, Yang H, Severinov K, Jin S. Transcriptional activation of Agrobacterium tumefaciens virulence gene promoters in Escherichia coli requires the A. tumefaciens RpoA gene, encoding the alpha subunit of RNA polymerase. J Bacteriol. 1999;181:4533-9 pubmed
    ..coli is unable to effectively interact with the VirG protein. This work provides the basis for future studies designed to examine vir gene expression as well as the T-DNA transfer process in E. coli. ..
  43. Michielse C, Ram A, Hooykaas P, van den Hondel C. Agrobacterium-mediated transformation of Aspergillus awamori in the absence of full-length VirD2, VirC2, or VirE2 leads to insertion of aberrant T-DNA structures. J Bacteriol. 2004;186:2038-45 pubmed
    ..Multicopy and truncated T-DNA structures were detected in the majority of the transformants obtained with the virC2 mutant, indicating that VirC2 plays a role in correct T-DNA processing and is required for single-copy T-DNA integration...
  44. Rao K, Bonanno J, Burley S, Swaminathan S. Crystal structure of glycerophosphodiester phosphodiesterase from Agrobacterium tumefaciens by SAD with a large asymmetric unit. Proteins. 2006;65:514-8 pubmed
  45. Eiamphungporn W, Charoenlap N, Vattanaviboon P, Mongkolsuk S. Agrobacterium tumefaciens soxR is involved in superoxide stress protection and also directly regulates superoxide-inducible expression of itself and a target gene. J Bacteriol. 2006;188:8669-73 pubmed publisher
    ..Taken together, the physiological role of soxR and the mechanism by which it regulates expression of target genes make the A. tumefaciens SoxR system different from other bacterial systems...
  46. Vattanaviboon P, Tanboon W, Mongkolsuk S. Physiological and expression analyses of Agrobacterium tumefaciens trxA, encoding thioredoxin. J Bacteriol. 2007;189:6477-81 pubmed publisher
    ..The trxA mutant showed a reduction in the aerobic growth rate and plating efficiency and was cytochrome c oxidase negative. Atypically, the mutant has decreased resistance to menadione but an increased H2O2 resistance phenotype...
  47. Zhao J, Binns A. Characterization of the mmsAB-araD1 (gguABC) genes of Agrobacterium tumefaciens. J Bacteriol. 2011;193:6586-96 pubmed publisher
  48. Bondage D, Lin J, Ma L, Kuo C, Lai E. VgrG C terminus confers the type VI effector transport specificity and is required for binding with PAAR and adaptor-effector complex. Proc Natl Acad Sci U S A. 2016;113:E3931-40 pubmed publisher
  49. Doty S, Chang M, Nester E. The chromosomal virulence gene, chvE, of Agrobacterium tumefaciens is regulated by a LysR family member. J Bacteriol. 1993;175:7880-6 pubmed
    ..chvE::lacZ expression was induced by L-arabinose, D-galactose, and D-fucose when gbpR was present. In the absence of inducer, GbpR repressed chvE::lacZ expression. In addition, GbpR negatively regulated its own expression...
  50. Vergunst A, van Lier M, den Dulk Ras A, St ve T, Ouwehand A, Hooykaas P. Positive charge is an important feature of the C-terminal transport signal of the VirB/D4-translocated proteins of Agrobacterium. Proc Natl Acad Sci U S A. 2005;102:832-7 pubmed publisher
    ..tumefaciens VirB/D4 system is hydrophilic and has a net positive charge with a consensus motif of R-X(7)-R-X-R-X-R-X-X(n)>...
  51. Shin D, Oganesyan N, Jancarik J, Yokota H, Kim R, Kim S. Crystal structure of a nicotinate phosphoribosyltransferase from Thermoplasma acidophilum. J Biol Chem. 2005;280:18326-35 pubmed publisher
    ..The structures of TaNAPRTase also provide valuable insight for other sequence subfamilies such as pre-B cell colony-enhancing factor, known to have nicotinamide phosphoribosyltransferase activity...
  52. Estruch J, Schell J, Spena A. The protein encoded by the rolB plant oncogene hydrolyses indole glucosides. EMBO J. 1991;10:3125-8 pubmed
    ..The combined action of these two genes therefore is expected to modulate the intracellular concentration of two of the main growth factors active in plants...
  53. Shirasu K, Morel P, Kado C. Characterization of the virB operon of an Agrobacterium tumefaciens Ti plasmid: nucleotide sequence and protein analysis. Mol Microbiol. 1990;4:1153-63 pubmed
    ..Specific differences in amino acid sequence encoded by VirB ORFs of nopaline and octopine Ti plasmid and a functional role of the gene products are discussed...
  54. Finan T, Gough C, Truchet G. Similarity between the Rhizobium meliloti fliP gene and pathogenicity-associated genes from animal and plant pathogens. Gene. 1995;152:65-7 pubmed
    ..It is likely that all of these proteins share a common functional role in the secretion of specific proteins from bacterial cells...
  55. Piper K, Beck von Bodman S, Farrand S. Conjugation factor of Agrobacterium tumefaciens regulates Ti plasmid transfer by autoinduction. Nature. 1993;362:448-50 pubmed publisher
  56. Baron C, Thorstenson Y, Zambryski P. The lipoprotein VirB7 interacts with VirB9 in the membranes of Agrobacterium tumefaciens. J Bacteriol. 1997;179:1211-8 pubmed
    ..tumefaciens verified that VirB7 is a lipoprotein associated with VirB9. VirB9 and VirB7 showed equal distribution between inner and outer membranes, in accord with their proposed association with the transmembrane VirB complex...
  57. Seufferheld M, Vieira M, Ruiz F, Rodrigues C, Moreno S, Docampo R. Identification of organelles in bacteria similar to acidocalcisomes of unicellular eukaryotes. J Biol Chem. 2003;278:29971-8 pubmed publisher
    ..The results suggest that acidocalcisomes arose before the prokaryotic and eukaryotic lineages diverged...
  58. Atmakuri K, Ding Z, Christie P. VirE2, a type IV secretion substrate, interacts with the VirD4 transfer protein at cell poles of Agrobacterium tumefaciens. Mol Microbiol. 2003;49:1699-713 pubmed
    ..Together, our findings establish for the first time that a protein substrate of a type IV secretion system is recruited to a member of the coupling protein superfamily...
  59. Gelvin S. AGROBACTERIUM AND PLANT GENES INVOLVED IN T-DNA TRANSFER AND INTEGRATION. Annu Rev Plant Physiol Plant Mol Biol. 2000;51:223-256 pubmed publisher
    ..In this article, I review what is currently known about the functions of virulence and plant proteins in several aspects of the Agrobacterium transformation process...
  60. Kim K, Kim H, Oh D, Cha S, Rhee S. Crystal structure of D-psicose 3-epimerase from Agrobacterium tumefaciens and its complex with true substrate D-fructose: a pivotal role of metal in catalysis, an active site for the non-phosphorylated substrate, and its conformational changes. J Mol Biol. 2006;361:920-31 pubmed publisher
  61. Dong A, Xu X, Edwards A, Chang C, Chruszcz M, Cuff M, et al. In situ proteolysis for protein crystallization and structure determination. Nat Methods. 2007;4:1019-21 pubmed publisher
    ..This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain...
  62. Saenkham P, Vattanaviboon P, Mongkolsuk S. Mutation in sco affects cytochrome c assembly and alters oxidative stress resistance in Agrobacterium tumefaciens. FEMS Microbiol Lett. 2009;293:122-9 pubmed publisher
    ..To summarize, we find that A. tumefaciens sco is required for the delivery of copper into active cytochrome c oxidase and in maintaining optimal resistance levels to oxidative stress...
  63. Tanboon W, Chuchue T, Vattanaviboon P, Mongkolsuk S. Inactivation of thioredoxin-like gene alters oxidative stress resistance and reduces cytochrome c oxidase activity in Agrobacterium tumefaciens. FEMS Microbiol Lett. 2009;295:110-6 pubmed publisher
    ..The presented data indicate that TlpA not only is involved in the proper assembly of cytochrome c but is also implicated in the bacterial oxidative stress response...
  64. Nawapan S, Charoenlap N, Charoenwuttitam A, Saenkham P, Mongkolsuk S, Vattanaviboon P. Functional and expression analyses of the cop operon, required for copper resistance in Agrobacterium tumefaciens. J Bacteriol. 2009;191:5159-68 pubmed publisher
    ..Altogether, the results demonstrate a crucial role for the copARZ operon as a component of the copper resistance machinery in A. tumefaciens...
  65. Costa E, Cho H, Winans S. Identification of amino acid residues of the pheromone-binding domain of the transcription factor TraR that are required for positive control. Mol Microbiol. 2009;73:341-51 pubmed publisher
  66. Andberg M, Maaheimo H, Boer H, Penttil M, Koivula A, Richard P. Characterization of a novel Agrobacterium tumefaciens galactarolactone cycloisomerase enzyme for direct conversion of D-galactarolactone to 3-deoxy-2-keto-L-threo-hexarate. J Biol Chem. 2012;287:17662-71 pubmed publisher
  67. Kuldau G, de Vos G, Owen J, McCaffrey G, Zambryski P. The virB operon of Agrobacterium tumefaciens pTiC58 encodes 11 open reading frames. Mol Gen Genet. 1990;221:256-66 pubmed
    ..A comparison of the two published octopine virB sequences with the nopaline sequence presented here is made...
  68. Goldberg S, Flick J, Rogers S. Nucleotide sequence of the tmr locus of Agrobacterium tumefaciens pTi T37 T-DNA. Nucleic Acids Res. 1984;12:4665-77 pubmed
    ..The 3' nontranslated regions show less than 50% homology as expected for the 3' regions of divergent related genes. The possible significance of areas of conserved sequences, particularly in the 5' regulatory regions, is discussed...
  69. Segal G, Ron E. The dnaKJ operon of Agrobacterium tumefaciens: transcriptional analysis and evidence for a new heat shock promoter. J Bacteriol. 1995;177:5952-8 pubmed
    ..This promoter may constitute the heat shock promoter of these alpha-purple proteobacteria...
  70. Kalogeraki V, Winans S. The octopine-type Ti plasmid pTiA6 of Agrobacterium tumefaciens contains a gene homologous to the chromosomal virulence gene acvB. J Bacteriol. 1995;177:892-7 pubmed
    ..Finally, mutations in these genes cannot be complemented extracellularly...
  71. Stasinopoulos S, Fisher P, Stone B, Stanisich V. Detection of two loci involved in (1-->3)-beta-glucan (curdlan) biosynthesis by Agrobacterium sp. ATCC31749, and comparative sequence analysis of the putative curdlan synthase gene. Glycobiology. 1999;9:31-41 pubmed
    ..Whatever the determinants of the linkage specificity of these beta-glucan synthases might be, these results raise the possibility that (1-->3)-beta-glucans and (1-->4)-beta-glucans are formed by related catalytic polypeptides...
  72. Ishii Y, Narimatsu Y, Iwasaki Y, Arai N, Kino K, Kirimura K. Reversible and nonoxidative gamma-resorcylic acid decarboxylase: characterization and gene cloning of a novel enzyme catalyzing carboxylation of resorcinol, 1,3-dihydroxybenzene, from Rhizobium radiobacter. Biochem Biophys Res Commun. 2004;324:611-20 pubmed publisher
    ..A site-directed mutagenesis study revealed the two histidine residues at positions of 164 and 218 in Rdc to be essential for the catalytic activities of decarboxylation of gamma-RA and carboxylation of RE...
  73. Lherbet C, Pojer F, Richard S, Noel J, Poulter C. Absence of substrate channeling between active sites in the Agrobacterium tumefaciens IspDF and IspE enzymes of the methyl erythritol phosphate pathway. Biochemistry. 2006;45:3548-53 pubmed publisher
    ..The time dependencies indicate that the enzyme-generated intermediates are not transferred from the IspD active site in IspDF to the active site of IspE or from the active site in IspE to the active site of the IspF module of IspDF...
  74. Harighi B. Genetic evidence for CheB- and CheR-dependent chemotaxis system in A. tumefaciens toward acetosyringone. Microbiol Res. 2009;164:634-41 pubmed publisher
    ..The chemosensory behaviour of mutants studied on swarm plates and in blind-well chemotaxis assay toward acetosyringone. Under the conditions tested, both CheR and CheB were essential for normal chemotaxis...
  75. Wu C, Lin J, Shaw G, Lai E. Acid-induced type VI secretion system is regulated by ExoR-ChvG/ChvI signaling cascade in Agrobacterium tumefaciens. PLoS Pathog. 2012;8:e1002938 pubmed publisher
    ..We present the first evidence of T6SS activation by an ExoR-ChvG/ChvI cascade and propose that acidity triggers ExoR degradation, thereby derepressing ChvG/ChvI to activate T6SS in A. tumefaciens...
  76. Zanker H, von Lintig J, Schr der J. Opine transport genes in the octopine (occ) and nopaline (noc) catabolic regions in Ti plasmids of Agrobacterium tumefaciens. J Bacteriol. 1992;174:841-9 pubmed
    ..The corresponding studies with 3H-labelled nopaline and the noc region cassettes indicated that the uptake of nopaline requires the putative transport genes and additional functions from the left part of the noc region...
  77. Christie P, Ward J, Gordon M, Nester E. A gene required for transfer of T-DNA to plants encodes an ATPase with autophosphorylating activity. Proc Natl Acad Sci U S A. 1989;86:9677-81 pubmed
    ..These findings suggest that phosphorylation may serve to activate a component(s) of the A. tumefaciens T-DNA transport apparatus and may also represent a general activation mechanism of other bacterial DNA transport systems...
  78. Sans N, Schr der G, Schr der J. The Noc region of Ti plasmid C58 codes for arginase and ornithine cyclodeaminase. Eur J Biochem. 1987;167:81-7 pubmed
    ..These three enzyme activities together constitute a catabolic pathway leading from nopaline through arginine and ornithine to proline...
  79. Bevan M, Barnes W, Chilton M. Structure and transcription of the nopaline synthase gene region of T-DNA. Nucleic Acids Res. 1983;11:369-85 pubmed
  80. Hwang I, Cook D, Farrand S. A new regulatory element modulates homoserine lactone-mediated autoinduction of Ti plasmid conjugal transfer. J Bacteriol. 1995;177:449-58 pubmed
  81. Tiburtius A, de Luca N, Hussain H, Johnston A. Expression of the exoY gene, required for exopolysaccharide synthesis in Agrobacterium, is activated by the regulatory ros gene. Microbiology. 1996;142 ( Pt 9):2621-9 pubmed
    ..Some of these mutations were dominant, causing merodiploids to be non-mucoid. exoY is linked to two genes, one encoding an omega-aminotransferase and the other encoding an aldehyde dehydrogenase...
  82. Liu Z, Jacobs M, Schaff D, McCullen C, Binns A. ChvD, a chromosomally encoded ATP-binding cassette transporter-homologous protein involved in regulation of virulence gene expression in Agrobacterium tumefaciens. J Bacteriol. 2001;183:3310-7 pubmed
    ..Constitutive expression of virG in the chvD mutant strain restored virulence, supporting the hypothesis that ChvD controls virulence through effects on virG expression...
  83. Bailey S, Ward D, Middleton R, Grossmann J, Zambryski P. Agrobacterium tumefaciens VirB8 structure reveals potential protein-protein interaction sites. Proc Natl Acad Sci U S A. 2006;103:2582-7 pubmed publisher
    ..The evolutionary conservation level for each residue was obtained from a data set of 84 VirB8 homologs and projected onto the protein structure to indicate conserved surface patches that likely contact other T4SS proteins...
  84. Bonnard G, Vincent F, Otten L. Sequence of Agrobacterium tumefaciens biotype III auxin genes. Plant Mol Biol. 1991;16:733-8 pubmed
  85. Endoh H, Hirayama T, Aoyama T, Oka A. Characterization of the virA gene of the agropine-type plasmid pRiA4 of Agrobacterium rhizogenes. FEBS Lett. 1990;271:28-32 pubmed
    ..These fused gene products became soluble or insoluble depending on the length of their lacZ moieties...
  86. Akiyoshi D, Regier D, Jen G, Gordon M. Cloning and nucleotide sequence of the tzs gene from Agrobacterium tumefaciens strain T37. Nucleic Acids Res. 1985;13:2773-88 pubmed
    ..A single new labelled protein of 27,200 daltons was detected in Escherichia coli maxicells expressing the cloned tzs gene. Significant sequence homology was observed between the tzs and the published tmr sequence from pTiT37...
  87. Ward J, Akiyoshi D, Regier D, Datta A, Gordon M, Nester E. Characterization of the virB operon from an Agrobacterium tumefaciens Ti plasmid. J Biol Chem. 1988;263:5804-14 pubmed
    ..We propose that the VirB proteins are involved in the formation of a transmembrane structure which mediates the passage of the transferred T-DNA molecule through the bacterial and plant cell membranes...
  88. Kemner J, Liang X, Nester E. The Agrobacterium tumefaciens virulence gene chvE is part of a putative ABC-type sugar transport operon. J Bacteriol. 1997;179:2452-8 pubmed
    ..tumefaciens on Kalanchoe diagremontiana; growth on 1 mM galactose, glucose, xylose, ribose, arabinose, fucose, or sucrose; or chemotaxis toward glucose, galactose, xylose, or arabinose...
  89. Kalogeraki V, Winans S. Wound-released chemical signals may elicit multiple responses from an Agrobacterium tumefaciens strain containing an octopine-type Ti plasmid. J Bacteriol. 1998;180:5660-7 pubmed
    ..Disruptions of virH, virK, virL, or virM did not affect tumorigenesis of Kalanchöe diagramontiana leaves or carrot disks, suggesting that they may play an entirely different role during pathogenesis...
  90. Ilari A, Stefanini S, Chiancone E, Tsernoglou D. The dodecameric ferritin from Listeria innocua contains a novel intersubunit iron-binding site. Nat Struct Biol. 2000;7:38-43 pubmed publisher
    ..The L. innocua ferritin site, however, is the first described so far that has ligands belonging to two different subunits and is not contained within a four-helix bundle...
  91. De Costa D, Suzuki K, Yoshida K. Structural and functional analysis of a putative gene cluster for palatinose transport on the linear chromosome of Agrobacterium tumefaciens MAFF301001. J Bacteriol. 2003;185:2369-73 pubmed
    ..Growth on trehalose and maltose was not influenced by the mutations...
  92. Matthysse A, Jaeckel P, Jeter C. attG and attC mutations of Agrobacterium tumefaciens are dominant negative mutations that block attachment and virulence. Can J Microbiol. 2008;54:241-7 pubmed publisher
    ..Thus attC and attG mutations are dominant negative mutations. The mechanism by which these mutations block attachment and virulence is unknown...