Genomes and Genes
Experts and Doctors on tertiary protein structure in Salt Lake City, Utah, United States
Locale: Salt Lake City, Utah, United States
Topic: tertiary protein structure
Publications104 found, 100 shown here
- Voth W, Yu Y, Takahata S, Kretschmann K, Lieb J, Parker R, et al. Forkhead proteins control the outcome of transcription factor binding by antiactivation. EMBO J. 2007;26:4324-34 pubmed..Thus Fkh proteins, which function initially to activate SWI5 and ACE2, subsequently function as Swi5-specific antiactivators. ..
- Sbia M, Parnell E, Yu Y, Olsen A, Kretschmann K, Voth W, et al. Regulation of the yeast Ace2 transcription factor during the cell cycle. J Biol Chem. 2008;283:11135-45 pubmed publisher..Our work suggests that the precise timing of Ace2 accumulation in the nucleus involves both a nuclear export sequence and a nuclear localization signal, whose activities are regulated by phosphorylation. ..
- Sheets M, Kyle J, Hanck D. The role of the putative inactivation lid in sodium channel gating current immobilization. J Gen Physiol. 2000;115:609-20 pubmed..Taken together with previous reports, these data also suggest that S4 in domain III contributes to charge immobilization only after binding of the inactivation lid. ..
- Fergestad T, Wu M, Schulze K, Lloyd T, Bellen H, Broadie K. Targeted mutations in the syntaxin H3 domain specifically disrupt SNARE complex function in synaptic transmission. J Neurosci. 2001;21:9142-50 pubmed..These data suggest that the same residues required for syntaxin-mediated calcium channel inhibition are required for the generation of fusion-competent vesicles in a neuronal-specific mechanism acting at synapses. ..
- Brown P, Hill C, Blair D. Crystal structure of the middle and C-terminal domains of the flagellar rotor protein FliG. EMBO J. 2002;21:3225-34 pubmed publisher..We propose a model for the organization of FliG-MC molecules in the flagellum, and suggest that CW/CCW switching might occur by movement of the C-terminal domain relative to other parts of FliG, under the control of FliM...
- Shiflett S, Kaplan J, Ward D. Chediak-Higashi Syndrome: a rare disorder of lysosomes and lysosome related organelles. Pigment Cell Res. 2002;15:251-7 pubmed..The identification of CHS1/Beige has defined a family of genes containing a common BEACH motif. The function of these proteins in vesicular trafficking remains unknown. ..
- Pornillos O, Alam S, Davis D, Sundquist W. Structure of the Tsg101 UEV domain in complex with the PTAP motif of the HIV-1 p6 protein. Nat Struct Biol. 2002;9:812-7 pubmed..The structure reveals the molecular basis of HIV PTAP late domain function and represents an attractive starting point for the design of novel inhibitors of virus budding. ..
- Phillips J, Whitby F, Stadtmueller B, Edwards C, Hill C, Kushner J. Two novel uroporphyrinogen decarboxylase (URO-D) mutations causing hepatoerythropoietic porphyria (HEP). Transl Res. 2007;149:85-91 pubmed..A second pregnancy occurred in this family, and in utero genotyping revealed a fetus heterozygous for the maternal nonsense mutation (URO-D genotype WT/Gln71Stop). A healthy infant was born with no clinical evidence of porphyria. ..
- VanDemark A, Kasten M, Ferris E, Heroux A, Hill C, Cairns B. Autoregulation of the rsc4 tandem bromodomain by gcn5 acetylation. Mol Cell. 2007;27:817-28 pubmed..Additional regulatory mechanisms are indicated as H3S10 phosphorylation inhibits Rsc4 binding to H3K14ac peptides. ..
- Morita E, Sandrin V, Chung H, Morham S, Gygi S, Rodesch C, et al. Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis. EMBO J. 2007;26:4215-27 pubmed..These experiments suggest that the ESCRT pathway may be recruited to facilitate analogous membrane fission events during HIV budding, MVB vesicle formation, and the abscission stage of cytokinesis. ..
- Topham M, Epand R. Mammalian diacylglycerol kinases: molecular interactions and biological functions of selected isoforms. Biochim Biophys Acta. 2009;1790:416-24 pubmed publisher..It is localized in several subcellular organelles, including the nucleus. The current state of our understanding of the properties and functions of these proteins is reviewed. ..
- Lee J, Ray R, Chien C. Cloning and expression of three zebrafish roundabout homologs suggest roles in axon guidance and cell migration. Dev Dyn. 2001;221:216-30 pubmed..They are also expressed in several nonneuronal tissues, including somites and fin buds. The timing and patterns of expression suggest roles for zebrafish robos in axon guidance and cell migration. Wiley-Liss, Inc. ..
- Braun T, Blair D. Targeted disulfide cross-linking of the MotB protein of Escherichia coli: evidence for two H(+) channels in the stator Complex. Biochemistry. 2001;40:13051-9 pubmed..Positions near the middle of the segment were inaccessible to sulhydryl reagents. Positions within 6-8 residues of either end, which includes residues around Asp32, were accessible. ..
- Eide E, Vielhaber E, Hinz W, Virshup D. The circadian regulatory proteins BMAL1 and cryptochromes are substrates of casein kinase Iepsilon. J Biol Chem. 2002;277:17248-54 pubmed..We conclude that CKIepsilon phosphorylates multiple circadian substrates and may exert its effects on circadian rhythm in part by a direct effect on BMAL1-dependent transcription. ..
- Schubert H, Raux E, Brindley A, Leech H, Wilson K, Hill C, et al. The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase. EMBO J. 2002;21:2068-75 pubmed..Analysis of mutant proteins suggests that both catalytic activities share a single active site, and that Asp141 plays an essential role in both dehydrogenase and chelatase processes. ..
- Wu J. C-erbB2 oncoprotein and its soluble ectodomain: a new potential tumor marker for prognosis early detection and monitoring patients undergoing Herceptin treatment. Clin Chim Acta. 2002;322:11-9 pubmed..Activation of p185 appears to be an early event in tumorigenesis for some cancers. It is possible that the ectodomain could be used as an early tumor marker detecting benign disease. ..
- Liu Y, Virshup D, White R, Hsu L. Regulation of BRCA1 phosphorylation by interaction with protein phosphatase 1alpha. Cancer Res. 2002;62:6357-61 pubmed..In addition, BRCA1 inhibits PP1alpha activity. Therefore, BRCA1 is both a substrate and a regulator of PP1alpha. The interaction between BRCA1 and PP1alpha thus may play a role in DNA damage repair and cell cycle progression. ..
- Evans Galea M, Blankman E, Myszka D, Bird A, Eide D, Winge D. Two of the five zinc fingers in the Zap1 transcription factor DNA binding domain dominate site-specific DNA binding. Biochemistry. 2003;42:1053-61 pubmed
- Mayer P, Xiang T, Niemi R, Anderson B. A hydrophobicity scale for the lipid bilayer barrier domain from peptide permeabilities: nonadditivities in residue contributions. Biochemistry. 2003;42:1624-36 pubmed..5 to -2.2 kcal/mol). These nonadditivities may be rationalized by considering the effects of peptide length and N-methylation on membrane-induced intramolecular hydrogen bonding leading to various folded conformations. ..
- Chen C. Recoverin and rhodopsin kinase. Adv Exp Med Biol. 2002;514:101-7 pubmed..This interaction imparts a negative feedback loop at the receptor level and may play an important role in light adaptation and in recovery. ..
- Amat di San Filippo C, Wang Y, Longo N. Functional domains in the carnitine transporter OCTN2, defective in primary carnitine deficiency. J Biol Chem. 2003;278:47776-84 pubmed..These studies indicate that multiple domains of the OCTN2 transporter are required for carnitine transport and identify transmembrane residues important for carnitine recognition. ..
- Fernandez D, Ghanta A, Kauffman G, Sanguinetti M. Physicochemical features of the HERG channel drug binding site. J Biol Chem. 2004;279:10120-7 pubmed..Together, these findings assign specific residues to receptor fields predicted by pharmacophore models of hERG channel blockers and provide a refined molecular understanding of the drug binding site. ..
- Sundquist W, Schubert H, Kelly B, Hill G, Holton J, Hill C. Ubiquitin recognition by the human TSG101 protein. Mol Cell. 2004;13:783-9 pubmed
- Gard D, Becker B, Josh Romney S. MAPping the eukaryotic tree of life: structure, function, and evolution of the MAP215/Dis1 family of microtubule-associated proteins. Int Rev Cytol. 2004;239:179-272 pubmed..The structure and function of MAP215/Dis1 proteins are discussed in relation to the evolution of this ancient family of microtubule-associated proteins. ..
- Zhang H, Richardson D, Roberts D, Utley R, Erdjument Bromage H, Tempst P, et al. The Yaf9 component of the SWR1 and NuA4 complexes is required for proper gene expression, histone H4 acetylation, and Htz1 replacement near telomeres. Mol Cell Biol. 2004;24:9424-36 pubmed..Taken together, these data indicate that Yaf9 may function in NuA4 and SWR1 complexes to help antagonize silencing near telomeres. ..
- Zheng Y, Brockie P, Mellem J, Madsen D, Walker C, Francis M, et al. SOL-1 is an auxiliary subunit that modulates the gating of GLR-1 glutamate receptors in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2006;103:1100-5 pubmed..Our analysis presents the possibility that glutamatergic signaling in other organisms may be similarly modified by SOL-1-like transmembrane proteins. ..
- Soss S, Flynn P. Functional implications for a prototypical K-turn binding protein from structural and dynamical studies of 15.5K. Biochemistry. 2007;46:14979-86 pubmed..Flexibility in fast time scale motions and the observation of limited intermediate and slow motions further characterize the free protein and may suggest local contributions to recognition and binding. ..
- Li L, Bagley D, Ward D, Kaplan J. Yap5 is an iron-responsive transcriptional activator that regulates vacuolar iron storage in yeast. Mol Cell Biol. 2008;28:1326-37 pubmed..These results show that Yap5 is an iron-sensing transcription factor and that iron regulates transcriptional activation. ..
- VanDemark A, Xin H, McCullough L, Rawlins R, Bentley S, Heroux A, et al. Structural and functional analysis of the Spt16p N-terminal domain reveals overlapping roles of yFACT subunits. J Biol Chem. 2008;283:5058-68 pubmed..The docking domain of H2A is identified as an important participant in maintaining stability during yFACT-mediated nucleosome reorganization, suggesting new models for the mechanism of this activity. ..
- Lin H, Burton D, Li L, Warner D, Phillips J, Ward D, et al. Gain-of-function mutations identify amino acids within transmembrane domains of the yeast vacuolar transporter Zrc1 that determine metal specificity. Biochem J. 2009;422:273-83 pubmed publisher..These results suggest that substrate selection involves co-operativity between transmembrane domains. ..
- Peterson C, Stoltzman C, Sighinolfi M, Han K, Ayer D. Glucose controls nuclear accumulation, promoter binding, and transcriptional activity of the MondoA-Mlx heterodimer. Mol Cell Biol. 2010;30:2887-95 pubmed publisher..Our experiments establish the mechanistic circuitry by which cells sense and respond transcriptionally to various intracellular glucose levels. ..
- Yu Y, Blair S, Gillespie D, Jensen R, Myszka D, Badran A, et al. Direct DNA methylation profiling using methyl binding domain proteins. Anal Chem. 2010;82:5012-9 pubmed publisher..Fitting skew normal probability density functions to our data, we estimate an accuracy of 97.5% for our method in identifying methylated CpG loci, which can be improved through optimization of probe design and surface density. ..
- Karren M, Coonrod E, Anderson T, Shaw J. The role of Fis1p-Mdv1p interactions in mitochondrial fission complex assembly. J Cell Biol. 2005;171:291-301 pubmed..These studies suggest that the concave binding surface of the Fis1p TPR-like fold interacts with Mdv1p during mitochondrial fission and that Mdv1p facilitates Dnm1p recruitment into functional fission complexes. ..
- Walker J, Poulter C. Synthesis and evaluation of 1-deoxy-D-xylulose 5-phosphate analogues as chelation-based inhibitors of methylerythritol phosphate synthase. J Org Chem. 2005;70:9955-9 pubmed..The carboxylate (1), methyl ester (3), amide (4), and alcohol (5) analogues were inhibitors with IC50's ranging from 0.25 to 1.0 mM. The hydroxamic acid (2) and amino (6) analogues did not inhibit the enzyme. ..
- Ball J, Dimaano C, Bilak A, Kurchan E, Zundel M, Ullman K. Sequence preference in RNA recognition by the nucleoporin Nup153. J Biol Chem. 2007;282:8734-40 pubmed..This information adds a new dimension to the interfaces that can contribute to recognition in mRNA export cargo selection and fate. ..
- Stuchell Brereton M, Skalicky J, Kieffer C, Karren M, Ghaffarian S, Sundquist W. ESCRT-III recognition by VPS4 ATPases. Nature. 2007;449:740-4 pubmed..Thus, our studies reveal how the VPS4 ATPases recognize their CHMP substrates to facilitate the membrane fission events required for the release of viruses, endosomal vesicles and daughter cells. ..
- Crawford A, Pino J, Beckerle M. Biochemical and molecular characterization of the chicken cysteine-rich protein, a developmentally regulated LIM-domain protein that is associated with the actin cytoskeleton. J Cell Biol. 1994;124:117-27 pubmed..We have performed quantitative Western immunoblot analysis of cCRP, zyxin, and alpha-actinin levels during embryogenesis. By this approach, we have demonstrated that the expression of cCRP is developmentally regulated. ..
- Dimaano C, Ball J, Prunuske A, Ullman K. RNA association defines a functionally conserved domain in the nuclear pore protein Nup153. J Biol Chem. 2001;276:45349-57 pubmed..Our findings identify a functionally conserved domain in Nup153 and suggest a role for RNA binding in Nup153 function at the nuclear pore. ..
- Whitby F, Phillips J, Hill C, McCoubrey W, Maines M. Crystal structure of a biliverdin IXalpha reductase enzyme-cofactor complex. J Mol Biol. 2002;319:1199-210 pubmed..This finding suggests that the dominant role in catalysis may be performed by hydride transfer from the cofactor, a process that may be promoted by proximity of the invariant residues Glu96, Glu123, and Glu126, to the nicotinamide ring. ..
- Fleischer T, Yun U, Ayer D. Identification and characterization of three new components of the mSin3A corepressor complex. Mol Cell Biol. 2003;23:3456-67 pubmed..Finally, all three SAPs bind to the HDAC-interaction domain (HID) of mSin3A, suggesting that the HID functions as the assembly interface for the mSin3A corepressor complex. ..
- Alam S, Langelier C, Whitby F, Koirala S, Robinson H, Hill C, et al. Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain. Nat Struct Mol Biol. 2006;13:1029-30 pubmed..The structure suggests how human ESCRT-II can couple recognition of ubiquitinated cargoes and endosomal phospholipids during MVB protein sorting. ..
- Morgan M, Thorburn J, Thomas L, Maxwell T, Brothman A, Thorburn A. An apoptosis signaling pathway induced by the death domain of FADD selectively kills normal but not cancerous prostate epithelial cells. Cell Death Differ. 2001;8:696-705 pubmed..Therefore, the death domain of FADD has a previously unrecognized role in cell survival that is epithelial-specific and defective in cancer cells. This FADD-dependent signaling pathway may be important in prostate carcinogenesis. ..
- Keller G, Ray E, Brown P, Winge D. Haa1, a protein homologous to the copper-regulated transcription factor Ace1, is a novel transcriptional activator. J Biol Chem. 2001;276:38697-702 pubmed..Overexpression of Haa1 does not compensate for cells lacking a functional Ace1. The lack of metalloregulation of Haa1 despite the strong sequence similarity to the copper regulatory domain of Ace1 is discussed. ..
- Mathews M, Schubert H, Whitby F, Alexander K, Schadick K, Bergonia H, et al. Crystal structure of human uroporphyrinogen III synthase. EMBO J. 2001;20:5832-9 pubmed..Additionally, six other highly conserved and titratable side chains were also mutated. In no case, however, did one of these mutations abolish enzyme activity, suggesting that the mechanism does not require acid/base catalysis. ..
- Renfranz P, Beckerle M. Doing (F/L)PPPPs: EVH1 domains and their proline-rich partners in cell polarity and migration. Curr Opin Cell Biol. 2002;14:88-103 pubmed
- Singer J, Shaw J. Mdm20 protein functions with Nat3 protein to acetylate Tpm1 protein and regulate tropomyosin-actin interactions in budding yeast. Proc Natl Acad Sci U S A. 2003;100:7644-9 pubmed..These combined results strongly suggest that Mdm20p-dependent, N-terminal acetylation of Tpm1p by the NatB complex is required for Tpm1p association with, and stabilization of, actin filaments and cables. ..
- Horikawa M, Kirkman N, Mayo K, Mulders S, Zhou J, Bondy C, et al. The mouse germ-cell-specific leucine-rich repeat protein NALP14: a member of the NACHT nucleoside triphosphatase family. Biol Reprod. 2005;72:879-89 pubmed..The structural attributes, expression pattern, and cellular localization of MATER and RNH2 largely conformed to those reported for NALP14. ..
- Smith R, Young J, Weis J, Weis J. Expression of the mouse fragilis gene products in immune cells and association with receptor signaling complexes. Genes Immun. 2006;7:113-21 pubmed..The mouse functional equivalent to human Leu-13 may not be single gene product, but instead may require the contribution of multiple fragilis proteins. ..
- Azam L, Yoshikami D, McIntosh J. Amino acid residues that confer high selectivity of the alpha6 nicotinic acetylcholine receptor subunit to alpha-conotoxin MII[S4A,E11A,L15A]. J Biol Chem. 2008;283:11625-32 pubmed publisher..Similar effects were seen with other alpha6-selective conotoxins, suggesting the general importance of thesealpha6 residues in conferring selective binding. ..
- Zhou Q, Ames P, Parkinson J. Mutational analyses of HAMP helices suggest a dynamic bundle model of input-output signalling in chemoreceptors. Mol Microbiol. 2009;73:801-14 pubmed publisher..This study shows how transmembrane signalling and HAMP input-output control could occur without the helix rotations central to the gearbox model. ..
- Jacquinet E, Rao N, Rao G, Hoidal J. Cloning, genomic organization, chromosomal assignment and expression of a novel mosaic serine proteinase: epitheliasin. FEBS Lett. 2000;468:93-100 pubmed..Epitheliasin is expressed primarily in the apical surfaces of renal tubular and airway epithelial cells. ..
- Eilers A, Sundwall E, Lin M, Sullivan A, Ayer D. A novel heterodimerization domain, CRM1, and 14-3-3 control subcellular localization of the MondoA-Mlx heterocomplex. Mol Cell Biol. 2002;22:8514-26 pubmed..Second, an extracellular signal(s) must overcome the cytoplasmic localization function imparted by CRM1 and 14-3-3 binding to the N terminus of MondoA. ..
- Luo B, Prescott S, Topham M. Protein kinase C alpha phosphorylates and negatively regulates diacylglycerol kinase zeta. J Biol Chem. 2003;278:39542-7 pubmed..Taken together, these results indicate that PKC alpha phosphorylates DGK zeta in cells, and this phosphorylation inhibits its kinase activity to remove cellular DAG, thereby affecting cell growth. ..
- Macbeth M, Lingam A, Bass B. Evidence for auto-inhibition by the N terminus of hADAR2 and activation by dsRNA binding. RNA. 2004;10:1563-71 pubmed..We propose that the N-terminal domain of hADAR2 contains sequences that cause auto-inhibition of the enzyme. Our results suggest activation requires binding to an RNA substrate long enough to accommodate interactions with both dsRBMs. ..
- dela Cruz R, Jairajpuri M, Bock S. Disruption of a tight cluster surrounding tyrosine 131 in the native conformation of antithrombin III activates it for factor Xa inhibition. J Biol Chem. 2006;281:31668-76 pubmed
- Biswas D, Dutta Biswas R, Stillman D. Chd1 and yFACT act in opposition in regulating transcription. Mol Cell Biol. 2007;27:6279-87 pubmed..Although human Chd1 has been shown to bind to H3-K4-Me, we discuss evidence arguing that yeast Chd1 binds to neither H3-K4-Me nor H3-K36-Me. ..
- Gardner A, Reichert E, Topham M, Stafforini D. Identification of a domain that mediates association of platelet-activating factor acetylhydrolase with high density lipoprotein. J Biol Chem. 2008;283:17099-106 pubmed publisher..These findings contribute to our understanding of the mechanisms responsible for association of human PAF-AH with HDL and may facilitate future studies aimed at precisely determining the function of PAF-AH in each lipoprotein particle. ..
- Pfaendtner J, Voth G. Molecular dynamics simulation and coarse-grained analysis of the Arp2/3 complex. Biophys J. 2008;95:5324-33 pubmed publisher..Finally, nucleotide-dependent coarse-grained models for Arp3 are developed that serve to further highlight the structural differences induced in Arp3 by nucleotide hydrolysis. ..
- Shestakova A, Hanono A, Drosner S, Curtiss M, Davies B, Katzmann D, et al. Assembly of the AAA ATPase Vps4 on ESCRT-III. Mol Biol Cell. 2010;21:1059-71 pubmed publisher..Vta1 in turn is predicted to cause a rearrangement of the Vps4 interactions that initiates the assembly of the active Vps4 oligomer. ..
- Chen J, Mitcheson J, Lin M, Sanguinetti M. Functional roles of charged residues in the putative voltage sensor of the HCN2 pacemaker channel. J Biol Chem. 2000;275:36465-71 pubmed
- McVey Ward D, Radisky D, Scullion M, Tuttle M, Vaughn M, Kaplan J. hVPS41 is expressed in multiple isoforms and can associate with vesicles through a RING-H2 finger motif. Exp Cell Res. 2001;267:126-34 pubmed..Together with information from the homologous yeast system, these results suggest that hVps41 may also be involved in the formation and fusion of transport vesicles from the Golgi. ..
- Fukushima N, Brisch E, Keegan B, Bleazard W, Shaw J. The GTPase effector domain sequence of the Dnm1p GTPase regulates self-assembly and controls a rate-limiting step in mitochondrial fission. Mol Biol Cell. 2001;12:2756-66 pubmed..Here we show that expression of the Dnm1 K705A protein in yeast enhances mitochondrial fission. Our results provide evidence that the GED region of a dynamin-related protein modulates a rate-limiting step in membrane fission. ..
- Bolger G, Peden A, Steele M, MacKenzie C, McEwan D, Wallace D, et al. Attenuation of the activity of the cAMP-specific phosphodiesterase PDE4A5 by interaction with the immunophilin XAP2. J Biol Chem. 2003;278:33351-63 pubmed..We suggest that XAP2 functionally interacts with PDE4A5 in cells. ..
- Kasten M, Szerlong H, Erdjument Bromage H, Tempst P, Werner M, Cairns B. Tandem bromodomains in the chromatin remodeler RSC recognize acetylated histone H3 Lys14. EMBO J. 2004;23:1348-59 pubmed..Taken together, Rsc4 bears essential tandem bromodomains that rely on H3 Lys14 acetylation to assist RSC complex for gene activation. ..
- Baker K, Beckstead R, Mangelsdorf D, Thummel C. Functional interactions between the Moses corepressor and DHR78 nuclear receptor regulate growth in Drosophila. Genes Dev. 2007;21:450-64 pubmed..The tight functional association between DHR78 and Moses provides a new paradigm for understanding the molecular mechanisms by which cofactors modulate nuclear receptor signaling pathways. ..
- Decher N, Renigunta V, Zuzarte M, Soom M, Heinemann S, Timothy K, et al. Impaired interaction between the slide helix and the C-terminus of Kir2.1: a novel mechanism of Andersen syndrome. Cardiovasc Res. 2007;75:748-57 pubmed..This interaction, which may be required for normal gating both in homomeric and heteromeric Kir2 channels, is disturbed by several mutations causing AS. ..
- Szerlong H, Hinata K, Viswanathan R, Erdjument Bromage H, Tempst P, Cairns B. The HSA domain binds nuclear actin-related proteins to regulate chromatin-remodeling ATPases. Nat Struct Mol Biol. 2008;15:469-76 pubmed publisher..Taken together, we define the role of the HSA domain and provide evidence for a regulatory relationship involving the ARP-HSA module and two new functional domains conserved in remodeler ATPases that contain ARPs. ..
- Garrone N, Blazer Yost B, Weiss R, Lalouel J, Rohrwasser A. A human polymorphism affects NEDD4L subcellular targeting by leading to two isoforms that contain or lack a C2 domain. BMC Cell Biol. 2009;10:26 pubmed publisher..Moreover, our observations regarding differential function of the NEDD4L isoforms may impact other aspects of physiology that involve this ubiquitin ligase. ..
- Bendahhou S, Cummins T, Tawil R, Waxman S, Ptacek L. Activation and inactivation of the voltage-gated sodium channel: role of segment S5 revealed by a novel hyperkalaemic periodic paralysis mutation. J Neurosci. 1999;19:4762-71 pubmed
- Hsu L, Doan T, White R. Identification of a gamma-tubulin-binding domain in BRCA1. Cancer Res. 2001;61:7713-8 pubmed..Thus, attenuating the interaction between BRCA1 and gamma-tubulin, and their association with mitotic centrosomes, may induce an increase of aneuploid cell population and contribute to tumorigenesis. ..
- Pope M, Porello S, David S. Escherichia coli apurinic-apyrimidinic endonucleases enhance the turnover of the adenine glycosylase MutY with G:A substrates. J Biol Chem. 2002;277:22605-15 pubmed..These results constitute the first evidence of an interaction between the MutY-product DNA complex and Endo IV or Exo III. Furthermore, they suggest a role for the C-terminal domain of MutY in mediating this interaction. ..
- Arenkiel B, Gaufo G, Capecchi M. Hoxb1 neural crest preferentially form glia of the PNS. Dev Dyn. 2003;227:379-86 pubmed
- Stuchell M, Garrus J, Muller B, Stray K, Ghaffarian S, McKinnon R, et al. The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding. J Biol Chem. 2004;279:36059-71 pubmed..Taken together, these experiments define new components of the human ESCRT-I complex and characterize several TSG101 protein/protein interactions required for HIV-1 budding and infectivity. ..
- Frederick R, McCaffery J, Cunningham K, Okamoto K, Shaw J. Yeast Miro GTPase, Gem1p, regulates mitochondrial morphology via a novel pathway. J Cell Biol. 2004;167:87-98 pubmed..2003. J. Biol. Chem. 278:6495-6502), Gem1p is not required for pheromone-induced yeast cell death. Thus, Gem1p defines a novel mitochondrial morphology pathway which may integrate cell signaling events with mitochondrial dynamics. ..
- Brown P, Mathews M, Joss L, Hill C, Blair D. Crystal structure of the flagellar rotor protein FliN from Thermotoga maritima. J Bacteriol. 2005;187:2890-902 pubmed publisher..On the basis of the present data and available electron microscopic images, we propose a model for the organization of FliN subunits in the C ring...
- Paul K, Nieto V, Carlquist W, Blair D, Harshey R. The c-di-GMP binding protein YcgR controls flagellar motor direction and speed to affect chemotaxis by a "backstop brake" mechanism. Mol Cell. 2010;38:128-39 pubmed publisher..Inhibition of motility and chemotaxis may represent a strategy to prepare for sedentary existence by disfavoring migration away from a substrate on which a biofilm is to be formed. ..
- Bibikov S, Barnes L, Gitin Y, Parkinson J. Domain organization and flavin adenine dinucleotide-binding determinants in the aerotaxis signal transducer Aer of Escherichia coli. Proc Natl Acad Sci U S A. 2000;97:5830-5 pubmed..One or both regions probably also play important roles in transmitting stimulus-induced conformational changes to the C-terminal flagellar signaling domain to trigger aerotactic behavioral responses. ..
- Edler M, Fernandez A, Lassota P, Ireland C, Barrows L. Inhibition of tubulin polymerization by vitilevuamide, a bicyclic marine peptide, at a site distinct from colchicine, the vinca alkaloids, and dolastatin 10. Biochem Pharmacol. 2002;63:707-15 pubmed..GTP binding was also found to be weakly affected by the presence of vitilevuamide. These results suggest the possibility that vitilevuamide inhibits tubulin polymerization via an interaction at a unique site. ..
- Francis A, David S. Escherichia coli MutY and Fpg utilize a processive mechanism for target location. Biochemistry. 2003;42:801-10 pubmed..A- and G.A-containing substrates. This suggests that the C-terminal domain of MutY plays an important role in the mechanism by which the enzyme detects OG.A base pairs in DNA. ..
- Kloeker S, Major M, Calderwood D, Ginsberg M, Jones D, Beckerle M. The Kindler syndrome protein is regulated by transforming growth factor-beta and involved in integrin-mediated adhesion. J Biol Chem. 2004;279:6824-33 pubmed..Additionally, kindlerin is required for normal cell spreading. Taken together, these data suggest a role for kindlerin in mediating cell processes that depend on integrins. ..
- Kondo Okamoto N, Shaw J, Okamoto K. Tetratricopeptide repeat proteins Tom70 and Tom71 mediate yeast mitochondrial morphogenesis. EMBO Rep. 2008;9:63-9 pubmed..Our data indicate an unexpected role for Tom70 in recruitment of soluble proteins to the mitochondrial surface, and indicate that Tom71 has a specialized role in Mfb1-mediated mitochondrial morphogenesis. ..
- Brown S, Sonntag D, Sanguinetti M. A highly conserved alanine in the S6 domain of the hERG1 K+ channel is required for normal gating. Cell Physiol Biochem. 2008;22:601-10 pubmed publisher..Thus, an Ala at position 653 in hERG1 is required for normal voltage dependence of channel gating and a charged residue in this position prevents channel closure. ..
- Perry M, Sachse F, Abbruzzese J, Sanguinetti M. PD-118057 contacts the pore helix of hERG1 channels to attenuate inactivation and enhance K+ conductance. Proc Natl Acad Sci U S A. 2009;106:20075-80 pubmed publisher..We conclude that direct interaction of PD-118057 with the pore helix attenuates fast P-type inactivation and increases open probability of hERG1 channels. ..
- Jette C, Peterson P, Sandoval I, Manos E, Hadley E, Ireland C, et al. The tumor suppressor adenomatous polyposis coli and caudal related homeodomain protein regulate expression of retinol dehydrogenase L. J Biol Chem. 2004;279:34397-405 pubmed..We propose a novel role for APC and CDX2 in controlling retinoic acid biosynthesis and in promoting a retinoid-induced program of colonocyte differentiation. ..
- Dong J, Opresko L, Chrisler W, Orr G, Quesenberry R, Lauffenburger D, et al. The membrane-anchoring domain of epidermal growth factor receptor ligands dictates their ability to operate in juxtacrine mode. Mol Biol Cell. 2005;16:2984-98 pubmed..Our data suggest that the membrane-anchoring domain of ligands selectively controls their ability to participate in juxtacrine signaling and thus, only a subclass of EGFR ligands can act in a juxtacrine mode. ..
- Parker G, Eckert D, Bass B. RDE-4 preferentially binds long dsRNA and its dimerization is necessary for cleavage of dsRNA to siRNA. RNA. 2006;12:807-18 pubmed..Using extracts from wild-type and rde-4 mutant C. elegans, we show that the C-terminal dimerization domain is required for the production of siRNA. Our findings suggest a model for RDE-4 function during the initiation of RNAi. ..
- Kaadige M, Ayer D. The polybasic region that follows the plant homeodomain zinc finger 1 of Pf1 is necessary and sufficient for specific phosphoinositide binding. J Biol Chem. 2006;281:28831-6 pubmed