Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in Salt Lake City, Utah, United States
Locale: Salt Lake City, Utah, United States
Topic: saccharomyces cerevisiae
Publications150 found, 100 shown here
- Voth W, Jiang Y, Stillman D. New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Yeast. 2003;20:985-93 pubmed..Marker swaps can also be used to change the selectable marker on plasmids, eliminating the need for subcloning. ..
- Rutherford J, Ojeda L, Balk J, Muhlenhoff U, Lill R, Winge D. Activation of the iron regulon by the yeast Aft1/Aft2 transcription factors depends on mitochondrial but not cytosolic iron-sulfur protein biogenesis. J Biol Chem. 2005;280:10135-40 pubmed..Although Aft1 and Aft2 sense a signal emanating from the Fe-S cluster biogenesis pathway, there is no indication that the proteins are inhibited by direct binding of an Fe-S cluster. ..
- Davis Kaplan S, Compton M, Flannery A, Ward D, Kaplan J, Stevens T, et al. PKR1 encodes an assembly factor for the yeast V-type ATPase. J Biol Chem. 2006;281:32025-35 pubmed..Our data indicate that Pkr1p functions together with the other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector. ..
- Khalimonchuk O, Bird A, Winge D. Evidence for a pro-oxidant intermediate in the assembly of cytochrome oxidase. J Biol Chem. 2007;282:17442-9 pubmed..Studies on the hydrogen peroxide sensitivity in CcO assembly mutants reveal new aspects of the CcO assembly process. ..
- Rigby K, Cobine P, Khalimonchuk O, Winge D. Mapping the functional interaction of Sco1 and Cox2 in cytochrome oxidase biogenesis. J Biol Chem. 2008;283:15015-22 pubmed publisher..In addition, the mutants failed to suppress the hydrogen peroxide sensitivity of sco1Delta cells. These studies implicate different surfaces on Sco1 for interaction or function with Cox17 and Cox2. ..
- Steffen J, Kang I, Portereiko M, Lloyd A, Drews G. AGL61 interacts with AGL80 and is required for central cell development in Arabidopsis. Plant Physiol. 2008;148:259-68 pubmed publisher..Together, these data suggest that AGL61 functions as a transcription factor and controls the expression of downstream genes during central cell development. ..
- Khalimonchuk O, Bestwick M, Meunier B, Watts T, Winge D. Formation of the redox cofactor centers during Cox1 maturation in yeast cytochrome oxidase. Mol Cell Biol. 2010;30:1004-17 pubmed publisher..Thus, formation of the heterobimetallic Cu(B):heme a(3) site likely occurs in the Shy1-containing Cox1 complex. ..
- Close D, Johnson S, Sdano M, McDonald S, Robinson H, Formosa T, et al. Crystal structures of the S. cerevisiae Spt6 core and C-terminal tandem SH2 domain. J Mol Biol. 2011;408:697-713 pubmed publisher..Overall, these findings provide a structural foundation for understanding how Spt6 encodes several distinct functions within a single polypeptide chain. ..
- Nguyen T, Lewandowska A, Choi J, Markgraf D, Junker M, Bilgin M, et al. Gem1 and ERMES do not directly affect phosphatidylserine transport from ER to mitochondria or mitochondrial inheritance. Traffic. 2012;13:880-90 pubmed publisher..Finally, we show that ERMES complexes are long-lived, and do not depend on the presence of Gem1. Our findings suggest that the ERMES complex may have primarily a structural role in maintaining mitochondrial morphology. ..
- Bricker D, Taylor E, Schell J, Orsak T, Boutron A, Chen Y, et al. A mitochondrial pyruvate carrier required for pyruvate uptake in yeast, Drosophila, and humans. Science. 2012;337:96-100 pubmed publisher..These data demonstrate that Mpc1 and Mpc2 form an essential part of the mitochondrial pyruvate carrier. ..
- Keener J, Babst M. Quality control and substrate-dependent downregulation of the nutrient transporter Fur4. Traffic. 2013;14:412-27 pubmed publisher..We propose that the LID-degron system is a conserved, chaperone-independent mechanism responsible for conformation-induced downregulation of many cell-surface transporters under physiological and pathological conditions. ..
- Lewandowska A, Macfarlane J, Shaw J. Mitochondrial association, protein phosphorylation, and degradation regulate the availability of the active Rab GTPase Ypt11 for mitochondrial inheritance. Mol Biol Cell. 2013;24:1185-95 pubmed publisher..We present a model that synthesizes these new features of Ypt11 function and regulation in mitochondrial inheritance. ..
- Cui T, Conte A, Fox J, Zara V, Winge D. Modulation of the respiratory supercomplexes in yeast: enhanced formation of cytochrome oxidase increases the stability and abundance of respiratory supercomplexes. J Biol Chem. 2014;289:6133-41 pubmed publisher..This study presents the first evidence showing that supercomplexes can be stabilized by the stimulated formation of CcO. ..
- Wang H, Reynolds Hager L, Stillman D. Genetic interactions between SIN3 mutations and the Saccharomyces cerevisiae transcriptional activators encoded by MCM1, STE12, and SWI1. Mol Gen Genet. 1994;245:675-85 pubmed..Thus, it is possible that the decreased transcription of STE6 in sin3 mutants is due to the combined effect of the diminished activities of Mcm1p and Ste12p...
- Jensen L, Posewitz M, Srinivasan C, Winge D. Mapping of the DNA binding domain of the copper-responsive transcription factor Mac1 from Saccharomyces cerevisiae. J Biol Chem. 1998;273:23805-11 pubmed..The elements appear to function synergistically. Increasing the number of elements yields more than additive enhancements in CTR1 expression. ..
- Li L, Kaplan J, Ward D. The glucose sensor Snf1 and the transcription factors Msn2 and Msn4 regulate transcription of the vacuolar iron importer gene CCC1 and iron resistance in yeast. J Biol Chem. 2017;292:15577-15586 pubmed publisher..In conclusion, we show that yeast have developed multiple transcriptional mechanisms to regulate Ccc1 expression and to protect against high cytosolic iron toxicity. ..
- Jiang Y, Dohrmann P, Stillman D. Genetic and physical interactions between yeast RGR1 and SIN4 in chromatin organization and transcriptional regulation. Genetics. 1995;140:47-54 pubmed..These results indicate that Sin4p and Rgr1p act together in vivo to organize chromatin structure and thus regulate transcription. ..
- Hoffman L, Rechsteiner M. Molecular cloning and expression of subunit 9 of the 26S proteasome. FEBS Lett. 1997;404:179-84 pubmed..In SDS-PAGE samples of the two complexes, the S9-specific antibodies bound a single 46 kDa subunit. Thus, a cDNA encoding a novel 26S protease subunit has been isolated, sequenced, and expressed. ..
- Kasten M, Stillman D. Identification of the Saccharomyces cerevisiae genes STB1-STB5 encoding Sin3p binding proteins. Mol Gen Genet. 1997;256:376-86 pubmed..We have shown previously that Sin3p is part of a large multiprotein complex, and we show here that Stb1p and Stb2p are present in this complex. ..
- Hermann G, Shaw J. Mitochondrial dynamics in yeast. Annu Rev Cell Dev Biol. 1998;14:265-303 pubmed
- Voth W, Richards J, Shaw J, Stillman D. Yeast vectors for integration at the HO locus. Nucleic Acids Res. 2001;29:E59-9 pubmed..We also describe several new bacterial polylinker vectors derived from pUC21 (ampicillin resistance) and pUK21 (kanamycin resistance). ..
- McIntyre T, Pontsler A, Silva A, St Hilaire A, Xu Y, Hinshaw J, et al. Identification of an intracellular receptor for lysophosphatidic acid (LPA): LPA is a transcellular PPARgamma agonist. Proc Natl Acad Sci U S A. 2003;100:131-6 pubmed..Moreover, LPA produced by stimulated plasma platelets activates PPARgamma in nucleated cells. ..
- Fleischer T, Yun U, Ayer D. Identification and characterization of three new components of the mSin3A corepressor complex. Mol Cell Biol. 2003;23:3456-67 pubmed..Finally, all three SAPs bind to the HDAC-interaction domain (HID) of mSin3A, suggesting that the HID functions as the assembly interface for the mSin3A corepressor complex. ..
- Szerlong H, Saha A, Cairns B. The nuclear actin-related proteins Arp7 and Arp9: a dimeric module that cooperates with architectural proteins for chromatin remodeling. EMBO J. 2003;22:3175-87 pubmed..We propose that Arp7/9 dimers function with DNA bending proteins to facilitate proper chromatin architecture and complex- complex interactions. ..
- Forster A, Whitby F, Hill C. The pore of activated 20S proteasomes has an ordered 7-fold symmetric conformation. EMBO J. 2003;22:4356-64 pubmed..Evolutionary considerations suggest that other activators might induce the same open proteasome conformation as seen with the 11S activator. ..
- Frederick R, McCaffery J, Cunningham K, Okamoto K, Shaw J. Yeast Miro GTPase, Gem1p, regulates mitochondrial morphology via a novel pathway. J Cell Biol. 2004;167:87-98 pubmed..2003. J. Biol. Chem. 278:6495-6502), Gem1p is not required for pheromone-induced yeast cell death. Thus, Gem1p defines a novel mitochondrial morphology pathway which may integrate cell signaling events with mitochondrial dynamics. ..
- Zhang H, Richardson D, Roberts D, Utley R, Erdjument Bromage H, Tempst P, et al. The Yaf9 component of the SWR1 and NuA4 complexes is required for proper gene expression, histone H4 acetylation, and Htz1 replacement near telomeres. Mol Cell Biol. 2004;24:9424-36 pubmed..Taken together, these data indicate that Yaf9 may function in NuA4 and SWR1 complexes to help antagonize silencing near telomeres. ..
- Kumanovics A, Poruk K, Osborn K, Ward D, Kaplan J. YKE4 (YIL023C) encodes a bidirectional zinc transporter in the endoplasmic reticulum of Saccharomyces cerevisiae. J Biol Chem. 2006;281:22566-74 pubmed
- Pierrel F, Khalimonchuk O, Cobine P, Bestwick M, Winge D. Coa2 is an assembly factor for yeast cytochrome c oxidase biogenesis that facilitates the maturation of Cox1. Mol Cell Biol. 2008;28:4927-39 pubmed publisher..The matrix-localized Coa2 protein may aid in stabilizing an early Cox1 intermediate containing the nuclear subunits Cox5a and Cox6. ..
- Smith P, Fox J, Winge D. Biogenesis of the cytochrome bc(1) complex and role of assembly factors. Biochim Biophys Acta. 2012;1817:276-86 pubmed publisher..This article is part of a Special Issue entitled: Biogenesis/Assembly of Respiratory Enzyme Complexes. ..
- Cardon C, Beck T, Hall M, Rutter J. PAS kinase promotes cell survival and growth through activation of Rho1. Sci Signal. 2012;5:ra9 pubmed publisher..This complex may integrate metabolic and signaling responses required for cell growth and survival in suboptimal conditions. ..
- Stadtmueller B, Kish Trier E, Ferrell K, Petersen C, Robinson H, Myszka D, et al. Structure of a proteasome Pba1-Pba2 complex: implications for proteasome assembly, activation, and biological function. J Biol Chem. 2012;287:37371-82 pubmed publisher..These findings extend understanding of proteasome interactions with HbYX motifs and suggest multiple roles for Pba1-Pba2 interactions throughout proteasome assembly and function. ..
- Voth W, Yu Y, Takahata S, Kretschmann K, Lieb J, Parker R, et al. Forkhead proteins control the outcome of transcription factor binding by antiactivation. EMBO J. 2007;26:4324-34 pubmed..Thus Fkh proteins, which function initially to activate SWI5 and ACE2, subsequently function as Swi5-specific antiactivators. ..
- Anderson M, Muehlbacher M, Street I, Proffitt J, Poulter C. Isopentenyl diphosphate:dimethylallyl diphosphate isomerase. An improved purification of the enzyme and isolation of the gene from Saccharomyces cerevisiae. J Biol Chem. 1989;264:19169-75 pubmed..This is the first reported isolation of the gene for IPP isomerase. ..
- Fish K, Cegielska A, Getman M, Landes G, Virshup D. Isolation and characterization of human casein kinase I epsilon (CKI), a novel member of the CKI gene family. J Biol Chem. 1995;270:14875-83 pubmed..Human CKI epsilon is a novel CKI isoform with properties that overlap those of previously described CKI isoforms. ..
- Stillman D, Dorland S, Yu Y. Epistasis analysis of suppressor mutations that allow HO expression in the absence of the yeast SW15 transcriptional activator. Genetics. 1994;136:781-8 pubmed..Finally, we show that complete suppression of the swi5 defect in HO expression by sin5 requires the wild-type ACE2 gene. This suggests that one function of SIN5 is to prevent ACE2, a SWI5 homolog, from activating HO expression. ..
- Li L, Kaplan J. Characterization of yeast methyl sterol oxidase (ERG25) and identification of a human homologue. J Biol Chem. 1996;271:16927-33 pubmed..These results suggest that the enzyme is regulated not by iron but by an end product of the ergosterol pathway. ..
- Li L, Kaplan J. Characterization of two homologous yeast genes that encode mitochondrial iron transporters. J Biol Chem. 1997;272:28485-93 pubmed..These results suggest that the mitochondria may act as a reservoir for iron that can be mobilized and used for cytosolic purposes. ..
- Li L, Kaplan J. Defects in the yeast high affinity iron transport system result in increased metal sensitivity because of the increased expression of transporters with a broad transition metal specificity. J Biol Chem. 1998;273:22181-7 pubmed..These results suggest that vacuolar function may not be required for transition metal sequestration. ..
- Ivanov I, Gesteland R, Matsufuji S, Atkins J. Programmed frameshifting in the synthesis of mammalian antizyme is +1 in mammals, predominantly +1 in fission yeast, but -2 in budding yeast. RNA. 1998;4:1230-8 pubmed..cerevisiae. S. pombe provides a good model system for genetic studies on the mechanism of at least this type of programmed mammalian frameshifting. ..
- Wittmeyer J, Joss L, Formosa T. Spt16 and Pob3 of Saccharomyces cerevisiae form an essential, abundant heterodimer that is nuclear, chromatin-associated, and copurifies with DNA polymerase alpha. Biochemistry. 1999;38:8961-71 pubmed..Some of the Spt16-Pob3 complex was found to copurify with the yeast DNA polymerase alpha/primase complex, further supporting a connection between Spt16-Pob3 and DNA replication. ..
- Bleazard W, McCaffery J, King E, Bale S, Mozdy A, Tieu Q, et al. The dynamin-related GTPase Dnm1 regulates mitochondrial fission in yeast. Nat Cell Biol. 1999;1:298-304 pubmed..These findings indicate that Dnm1 regulates mitochondrial fission, assembling on the cytoplasmic face of mitochondrial tubules at sites at which division will occur. ..
- Singer J, Hermann G, Shaw J. Suppressors of mdm20 in yeast identify new alleles of ACT1 and TPM1 predicted to enhance actin-tropomyosin interactions. Genetics. 2000;156:523-34 pubmed..The characteristics of the mutant ACT1 and TPM1 alleles and their potential effects on protein structure and binding are discussed. ..
- Chen O, Kaplan J. YFH1-mediated iron homeostasis is independent of mitochondrial respiration. FEBS Lett. 2001;509:131-4 pubmed..We conclude that the effect of Yfh1p on mitochondrial iron metabolism is independent of respiratory activity. ..
- Bolger G, Peden A, Steele M, MacKenzie C, McEwan D, Wallace D, et al. Attenuation of the activity of the cAMP-specific phosphodiesterase PDE4A5 by interaction with the immunophilin XAP2. J Biol Chem. 2003;278:33351-63 pubmed..We suggest that XAP2 functionally interacts with PDE4A5 in cells. ..
- Li L, Vulpe C, Kaplan J. Functional studies of hephaestin in yeast: evidence for multicopper oxidase activity in the endocytic pathway. Biochem J. 2003;375:793-8 pubmed
- Horng Y, Cobine P, Maxfield A, Carr H, Winge D. Specific copper transfer from the Cox17 metallochaperone to both Sco1 and Cox11 in the assembly of yeast cytochrome C oxidase. J Biol Chem. 2004;279:35334-40 pubmed..Metallation of these domains was strictly dependent on the co-expression of Cox17. Thus, Cox17 represents a novel copper chaperone that delivers copper to two proteins. ..
- Zhang H, Roberts D, Cairns B. Genome-wide dynamics of Htz1, a histone H2A variant that poises repressed/basal promoters for activation through histone loss. Cell. 2005;123:219-31 pubmed..We suggest that Htz1-bearing nucleosomes are deposited at repressed/basal promoters but facilitate activation through their susceptibility to loss, thereby helping to expose promoter DNA. ..
- Kondo Okamoto N, Ohkuni K, Kitagawa K, McCaffery J, Shaw J, Okamoto K. The novel F-box protein Mfb1p regulates mitochondrial connectivity and exhibits asymmetric localization in yeast. Mol Biol Cell. 2006;17:3756-67 pubmed..We propose that Mfb1p operates in a novel pathway regulating mitochondrial tubular connectivity. ..
- McCullock S, Kinard T, McCullough L, Formosa T. blm3-1 is an allele of UBP3, a ubiquitin protease that appears to act during transcription of damaged DNA. J Mol Biol. 2006;363:660-72 pubmed
- Curtiss M, Jones C, Babst M. Efficient cargo sorting by ESCRT-I and the subsequent release of ESCRT-I from multivesicular bodies requires the subunit Mvb12. Mol Biol Cell. 2007;18:636-45 pubmed..Together, these observations suggest that Mvb12 may function in regulating the interactions of ESCRT-I with cargo and other proteins of the ESCRT machinery to efficiently coordinate cargo sorting and release of ESCRT-I from the MVB. ..
- Kondo Okamoto N, Shaw J, Okamoto K. Tetratricopeptide repeat proteins Tom70 and Tom71 mediate yeast mitochondrial morphogenesis. EMBO Rep. 2008;9:63-9 pubmed..Our data indicate an unexpected role for Tom70 in recruitment of soluble proteins to the mitochondrial surface, and indicate that Tom71 has a specialized role in Mfb1-mediated mitochondrial morphogenesis. ..
- Biswas D, Takahata S, Xin H, Dutta Biswas R, Yu Y, Formosa T, et al. A role for Chd1 and Set2 in negatively regulating DNA replication in Saccharomyces cerevisiae. Genetics. 2008;178:649-59 pubmed publisher..The pob3 defect in S-phase progression is suppressed by set2 or chd1 mutations, suggesting that Set2 and Chd1 have specific roles in negatively regulating DNA replication. ..
- Lin H, Kumanovics A, Nelson J, Warner D, Ward D, Kaplan J. A single amino acid change in the yeast vacuolar metal transporters ZRC1 and COT1 alters their substrate specificity. J Biol Chem. 2008;283:33865-73 pubmed publisher..These mutations are within the second hydrophobic domain of the transporters and show the essential nature of this domain in the specificity of metal transport. ..
- Shestakova A, Hanono A, Drosner S, Curtiss M, Davies B, Katzmann D, et al. Assembly of the AAA ATPase Vps4 on ESCRT-III. Mol Biol Cell. 2010;21:1059-71 pubmed publisher..Vta1 in turn is predicted to cause a rearrangement of the Vps4 interactions that initiates the assembly of the active Vps4 oligomer. ..
- Bui H, Karren M, Bhar D, Shaw J. A novel motif in the yeast mitochondrial dynamin Dnm1 is essential for adaptor binding and membrane recruitment. J Cell Biol. 2012;199:613-22 pubmed publisher..Based on the variability of Insert B sequences and adaptor proteins, we propose that Insert B domains and mitochondrial adaptors have coevolved to meet the unique requirements for mitochondrial fission of different organisms. ..
- Shestakova A, Curtiss M, Davies B, Katzmann D, Babst M. The linker region plays a regulatory role in assembly and activity of the Vps4 AAA ATPase. J Biol Chem. 2013;288:26810-9 pubmed publisher..This structure would allow the Vps4 complex to engage ESCRT-III subunits with both the pore and the MIT domain simultaneously, which might be essential for the ATP-driven disassembly of ESCRT-III. ..
- Biswas D, Takahata S, Stillman D. Different genetic functions for the Rpd3(L) and Rpd3(S) complexes suggest competition between NuA4 and Rpd3(S). Mol Cell Biol. 2008;28:4445-58 pubmed publisher..Our results suggest that the Rpd3(L) and Rpd3(S) complexes have distinct functions in vivo and that the relative amounts of the two forms alter the effectiveness of other chromatin-altering complexes, such as FACT and NuA4. ..
- Ferrell K, Deveraux Q, van Nocker S, Rechsteiner M. Molecular cloning and expression of a multiubiquitin chain binding subunit of the human 26S protease. FEBS Lett. 1996;381:143-8 pubmed..Moreover, expression of the isolated cDNA in E. coli results in a recombinant protein with an apparent molecular mass and multiubiquitin binding properties that match those of human S5a obtained from the purified 26S enzyme. ..
- Formosa T, Nittis T. Suppressors of the temperature sensitivity of DNA polymerase alpha mutations in Saccharomyces cerevisiae. Mol Gen Genet. 1998;257:461-8 pubmed..We also describe a transposon-mediated strategy that should be generally effective for rapid characterization of multicopy suppressors. ..
- Keller G, Gross C, Kelleher M, Winge D. Functional independence of the two cysteine-rich activation domains in the yeast Mac1 transcription factor. J Biol Chem. 2000;275:29193-9 pubmed..Thus, the two Cys-rich motifs appear to function independently. The C1 motif appears to be a functional copper-regulatory domain. ..
- Heaton D, Nittis T, Srinivasan C, Winge D. Mutational analysis of the mitochondrial copper metallochaperone Cox17. J Biol Chem. 2000;275:37582-7 pubmed..Thus, only three cysteinyl residues are important for the ligation of three Cu(I) ions. A novel mode of Cu(I) binding is predicted. ..
- Keller G, Ray E, Brown P, Winge D. Haa1, a protein homologous to the copper-regulated transcription factor Ace1, is a novel transcriptional activator. J Biol Chem. 2001;276:38697-702 pubmed..Overexpression of Haa1 does not compensate for cells lacking a functional Ace1. The lack of metalloregulation of Haa1 despite the strong sequence similarity to the copper regulatory domain of Ace1 is discussed. ..
- McBride H, Sil A, Measday V, Yu Y, Moffat J, Maxon M, et al. The protein kinase Pho85 is required for asymmetric accumulation of the Ash1 protein in Saccharomyces cerevisiae. Mol Microbiol. 2001;42:345-53 pubmed..We observe that Ash1 protein is phosphorylated by Pho85 in vitro and that Ash1 stability increases in a pho85 mutant. These data suggest that phosphorylation of Ash1 by Pho85 governs stability of Ash1 protein. ..
- Formosa T, Ruone S, Adams M, Olsen A, Eriksson P, Yu Y, et al. Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure. Genetics. 2002;162:1557-71 pubmed..Mutations that impair the reassembly activity cause chromatin to accumulate in an abnormally disrupted state, imposing a requirement for a nucleosome reassembly function that we propose is provided by Hir/Hpc proteins. ..
- Decher N, Bundis F, Vajna R, Steinmeyer K. KCNE2 modulates current amplitudes and activation kinetics of HCN4: influence of KCNE family members on HCN4 currents. Pflugers Arch. 2003;446:633-40 pubmed..KCNE2, by modulating I(f) or I(h) currents, might thus contribute to the electrophysiological diversity of known pacemaking currents in the heart and brain. ..
- Eriksson P, Thomas L, Thorburn A, Stillman D. pRS yeast vectors with a LYS2 marker. Biotechniques. 2004;36:212-3 pubmed
- Phillips J, Whitby F, Warby C, Labbe P, Yang C, Pflugrath J, et al. Crystal structure of the oxygen-dependant coproporphyrinogen oxidase (Hem13p) of Saccharomyces cerevisiae. J Biol Chem. 2004;279:38960-8 pubmed..The structure therefore suggests residues that likely play critical roles in catalysis and explains the deleterious effect of many of the mutations associated with the disease hereditary coproporphyria. ..
- Keller G, Bird A, Winge D. Independent metalloregulation of Ace1 and Mac1 in Saccharomyces cerevisiae. Eukaryot Cell. 2005;4:1863-71 pubmed..Likewise, high expression of a copper-binding, non-DNA-binding Mac1 mutant is without effect on the copper activation of Ace1. Thus, metalloregulation of Ace1 and Mac1 occurs independently. ..
- Langelier C, von Schwedler U, Fisher R, De Domenico I, White P, Hill C, et al. Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release. J Virol. 2006;80:9465-80 pubmed..These observations indicate that there are probably multiple pathways for protein sorting/MVB vesicle formation in human cells and that HIV-1 does not utilize an ESCRT-II-dependent pathway to leave the cell. ..
- Li L, Bagley D, Ward D, Kaplan J. Yap5 is an iron-responsive transcriptional activator that regulates vacuolar iron storage in yeast. Mol Cell Biol. 2008;28:1326-37 pubmed..These results show that Yap5 is an iron-sensing transcription factor and that iron regulates transcriptional activation. ..
- Sbia M, Parnell E, Yu Y, Olsen A, Kretschmann K, Voth W, et al. Regulation of the yeast Ace2 transcription factor during the cell cycle. J Biol Chem. 2008;283:11135-45 pubmed publisher..Our work suggests that the precise timing of Ace2 accumulation in the nucleus involves both a nuclear export sequence and a nuclear localization signal, whose activities are regulated by phosphorylation. ..
- Gonciarz M, Whitby F, Eckert D, Kieffer C, Heroux A, Sundquist W, et al. Biochemical and structural studies of yeast Vps4 oligomerization. J Mol Biol. 2008;384:878-95 pubmed publisher..Our mutational analyses demonstrate that pore loop 2 residues Arg241 and Arg251 are required for efficient HIV-1 budding, thereby supporting a role for this "arginine collar" in Vps4 function. ..
- Parnell K, Bass B. Functional redundancy of yeast proteins Reh1 and Rei1 in cytoplasmic 60S subunit maturation. Mol Cell Biol. 2009;29:4014-23 pubmed publisher..We observe that strains lacking both Reh1 and Rei1 accumulate salt-labile 60S subunits, suggesting that Reh1/Rei1 is necessary for the cytoplasmic 60S subunit to adopt its mature, stable form. ..
- Cardon C, Rutter J. PAS kinase: integrating nutrient sensing with nutrient partitioning. Semin Cell Dev Biol. 2012;23:626-30 pubmed publisher..We propose that, like other kinases possessing these properties-AMPK and TOR, PAS kinase might be target for therapy of diabetes, obesity and cancer. ..
- Kemble D, Whitby F, Robinson H, McCullough L, Formosa T, Hill C. Structure of the Spt16 middle domain reveals functional features of the histone chaperone FACT. J Biol Chem. 2013;288:10188-94 pubmed publisher..Our structure provides a framework for interpreting a large body of genetic data regarding the physiological functions of FACT, including the identification of potential interaction surfaces for binding histones or other proteins. ..
- Heo J, Nielson J, Dephoure N, Gygi S, Rutter J. Intramolecular interactions control Vms1 translocation to damaged mitochondria. Mol Biol Cell. 2013;24:1263-73 pubmed publisher..These studies define cellular and biochemical mechanisms by which Vms1 locali-zation to mitochondria is controlled to enable an efficient protein quality control system. ..
- Bui H, Shaw J. Dynamin assembly strategies and adaptor proteins in mitochondrial fission. Curr Biol. 2013;23:R891-9 pubmed publisher..Here, we review the structure and assembly properties of the yeast and mammalian mitochondrial dynamins and discuss what is known about the activities of their adaptor proteins. ..
- Babcock M, de Silva D, Oaks R, Davis Kaplan S, Jiralerspong S, Montermini L, et al. Regulation of mitochondrial iron accumulation by Yfh1p, a putative homolog of frataxin. Science. 1997;276:1709-12 pubmed..Human frataxin also was shown to be a mitochondrial protein. Characterizing the mechanism by which YFH1 regulates iron homeostasis in yeast may help to define the pathologic process leading to cell damage in Friedreich's ataxia. ..
- Formosa T, Nittis T. Dna2 mutants reveal interactions with Dna polymerase alpha and Ctf4, a Pol alpha accessory factor, and show that full Dna2 helicase activity is not essential for growth. Genetics. 1999;151:1459-70 pubmed..Dna2 therefore appears to act in repair or lagging strand synthesis together with Pol alpha and Ctf4, in a role that is optimal with, but does not require, full helicase activity. ..
- Angus Hill M, Dutnall R, Tafrov S, Sternglanz R, Ramakrishnan V. Crystal structure of the histone acetyltransferase Hpa2: A tetrameric member of the Gcn5-related N-acetyltransferase superfamily. J Mol Biol. 1999;294:1311-25 pubmed..We discuss implications of the Hpa2 structure for the catalytic mechanism of the GNAT enzymes and the opportunity for multiple histone tail modification created by the tetrameric Hpa2 structure. ..
- Ardon O, Bussey H, Philpott C, Ward D, Davis Kaplan S, Verroneau S, et al. Identification of a Candida albicans ferrichrome transporter and its characterization by expression in Saccharomyces cerevisiae. J Biol Chem. 2001;276:43049-55 pubmed
- Karren M, Coonrod E, Anderson T, Shaw J. The role of Fis1p-Mdv1p interactions in mitochondrial fission complex assembly. J Cell Biol. 2005;171:291-301 pubmed..These studies suggest that the concave binding surface of the Fis1p TPR-like fold interacts with Mdv1p during mitochondrial fission and that Mdv1p facilitates Dnm1p recruitment into functional fission complexes. ..
- Biswas D, Yu Y, Mitra D, Stillman D. Genetic interactions between Nhp6 and Gcn5 with Mot1 and the Ccr4-Not complex that regulate binding of TATA-binding protein in Saccharomyces cerevisiae. Genetics. 2006;172:837-49 pubmed..Chromatin immunoprecipitation experiments show decreased binding of TBP to promoters in mot1 mutants and a further decrease when combined with either nhp6ab or gcn5 mutations. ..
- McDonald S, Close D, Xin H, Formosa T, Hill C. Structure and biological importance of the Spn1-Spt6 interaction, and its regulatory role in nucleosome binding. Mol Cell. 2010;40:725-35 pubmed publisher..These data thereby reveal the structural and biochemical bases of molecular interactions that function in the maintenance of chromatin structure. ..
- McCright B, Virshup D. Identification of a new family of protein phosphatase 2A regulatory subunits. J Biol Chem. 1995;270:26123-8 pubmed..The identification of this novel phosphatase regulator gene family will facilitate future studies on the control of protein dephosphorylation and the role of PP2A in cellular function. ..
- Martins L, Jensen L, Simon J, Keller G, Winge D, Simons J. Metalloregulation of FRE1 and FRE2 homologs in Saccharomyces cerevisiae. J Biol Chem. 1998;273:23716-21 pubmed..From the three Mac1-responsive elements in FRE7, a new consensus sequence for Mac1 binding can be established as TTTGC(T/G)C(A/G). ..
- Biswas D, Imbalzano A, Eriksson P, Yu Y, Stillman D. Role for Nhp6, Gcn5, and the Swi/Snf complex in stimulating formation of the TATA-binding protein-TFIIA-DNA complex. Mol Cell Biol. 2004;24:8312-21 pubmed..Consistent with the idea that Nhp6, Gcn5, and Swi/Snf have overlapping functions in vivo, nhp6a nhp6b gcn5 mutants had a severe growth defect, and mutations in both nhp6a nhp6b swi2 and gcn5 swi2 strains were lethal. ..
- Khalimonchuk O, Rigby K, Bestwick M, Pierrel F, Cobine P, Winge D. Pet191 is a cytochrome c oxidase assembly factor in Saccharomyces cerevisiae. Eukaryot Cell. 2008;7:1427-31 pubmed publisher..The import of Pet191 differs from that of other twin-Cx(9)C motif class of proteins in being independent of the Mia40 pathway. ..
- Wenzel D, Klevit R. Following Ariadne's thread: a new perspective on RBR ubiquitin ligases. BMC Biol. 2012;10:24 pubmed publisher..Re-evaluation of RBR ligases as RING/HECT E3s provokes new questions and challenges the field. ..
- Wittmeyer J, Formosa T. The Saccharomyces cerevisiae DNA polymerase alpha catalytic subunit interacts with Cdc68/Spt16 and with Pob3, a protein similar to an HMG1-like protein. Mol Cell Biol. 1997;17:4178-90 pubmed..Based on these results and because Cdc68 has been implicated as a regulator of chromatin structure, we postulate that polymerase alpha may interact with these proteins to gain access to its template or to origins of replication in vivo. ..