Experts and Doctors on protein subunits in Salt Lake City, Utah, United States


Locale: Salt Lake City, Utah, United States
Topic: protein subunits

Top Publications

  1. Hone A, Scadden M, Gajewiak J, Christensen S, Lindstrom J, McIntosh J. ?-Conotoxin PeIA[S9H,V10A,E14N] potently and selectively blocks ?6?2?3 versus ?6?4 nicotinic acetylcholine receptors. Mol Pharmacol. 2012;82:972-82 pubmed publisher
    ..096 ± 0.001 min(-1) and a k(on) of 0.23 ± 0.019 min(-1) M(-9). The synthesis of PeIA[S9H,V10A,E14N] demonstrates that ligands can be developed to discriminate between ?6?2 and ?6?4 nAChRs. ..
  2. Simeone T, Otto J, Wilcox K, White H. Felbamate is a subunit selective modulator of recombinant gamma-aminobutyric acid type A receptors expressed in Xenopus oocytes. Eur J Pharmacol. 2006;552:31-5 pubmed
    ..Regional distributions of GABA(A) receptor subunits suggest that felbamate may differentially modulate distinct inhibitory circuits, a possibility that may have relevance to felbamate efficacy in refractory epilepsies. ..
  3. Zhang H, Limphong P, Pieper J, Liu Q, Rodesch C, Christians E, et al. Glutathione-dependent reductive stress triggers mitochondrial oxidation and cytotoxicity. FASEB J. 2012;26:1442-51 pubmed publisher
    ..Taken together, our findings reveal a novel mechanism by which GSH-dependent reductive stress triggers mitochondrial oxidation and cytotoxicity. ..
  4. Vargas J, Takahashi D, Thomson K, Wilcox K. The expression of kainate receptor subunits in hippocampal astrocytes after experimentally induced status epilepticus. J Neuropathol Exp Neurol. 2013;72:919-32 pubmed publisher
    ..These results demonstrate that astrocytes begin to express KARs after seizure activity and suggest that their expression may contribute to the pathophysiology of epilepsy. ..
  5. Li X, Scuderi A, Letsou A, Virshup D. B56-associated protein phosphatase 2A is required for survival and protects from apoptosis in Drosophila melanogaster. Mol Cell Biol. 2002;22:3674-84 pubmed
  6. Singh N, Pappas C, Dahle E, Claes L, Pruess T, De Jonghe P, et al. A role of SCN9A in human epilepsies, as a cause of febrile seizures and as a potential modifier of Dravet syndrome. PLoS Genet. 2009;5:e1000649 pubmed publisher
    ..This study provides evidence for a role of SCN9A in human epilepsies, both as a cause of FS and as a partner with SCN1A mutations. ..
  7. Weiss R, Baker T, Cannon D, von Niederhausern A, Dunn D, Matsunami N, et al. A candidate gene approach identifies the CHRNA5-A3-B4 region as a risk factor for age-dependent nicotine addiction. PLoS Genet. 2008;4:e1000125 pubmed publisher
    ..The identification of an age-dependent susceptibility haplotype reinforces the importance of preventing early exposure to tobacco through public health policies. ..
  8. Ellison M, Feng Z, Park A, Zhang X, Olivera B, McIntosh J, et al. Alpha-RgIA, a novel conotoxin that blocks the alpha9alpha10 nAChR: structure and identification of key receptor-binding residues. J Mol Biol. 2008;377:1216-27 pubmed publisher
    ..Our findings contribute to a better understanding of the molecular basis for antagonism of the alpha9alpha10 nAChR subtype, which is a target for the development of analgesics for the treatment of chronic neuropathic pain. ..
  9. Mellem J, Brockie P, Zheng Y, Madsen D, Maricq A. Decoding of polymodal sensory stimuli by postsynaptic glutamate receptors in C. elegans. Neuron. 2002;36:933-44 pubmed
    ..Furthermore, analysis of the EGL-3 proprotein convertase provides additional evidence that polymodal signaling in C. elegans occurs via the differential activation of postsynaptic glutamate receptor subtypes. ..

More Information


  1. Magdaleno S, Northcutt G, Curran T, Kurschner C. mPPP1R16B is a novel mouse protein phosphatase 1 targeting subunit whose mRNA is located in cell bodies and dendrites of neurons in four distinct regions of the brain. Brain Res Gene Expr Patterns. 2002;1:143-9 pubmed
    ..In situ hybridization revealed that Ppp1r16b message is found in both the cell bodies and the dendrites in Purkinje cells of the cerebellum and granule neurons of the dentate gyrus. ..
  2. Hone A, Ruiz M, Scadden M, Christensen S, Gajewiak J, Azam L, et al. Positional scanning mutagenesis of ?-conotoxin PeIA identifies critical residues that confer potency and selectivity for ?6/?3?2?3 and ?3?2 nicotinic acetylcholine receptors. J Biol Chem. 2013;288:25428-39 pubmed publisher
    ..The results of these studies provide molecular insights into designing ligands that selectively target ?6?2* nAChRs. ..
  3. Hao H, Khalimonchuk O, Schraders M, Dephoure N, Bayley J, Kunst H, et al. SDH5, a gene required for flavination of succinate dehydrogenase, is mutated in paraganglioma. Science. 2009;325:1139-42 pubmed publisher
    ..Thus, a mitochondrial proteomics analysis in yeast has led to the discovery of a human tumor susceptibility gene. ..
  4. Paul K, Blair D. Organization of FliN subunits in the flagellar motor of Escherichia coli. J Bacteriol. 2006;188:2502-11 pubmed
    ..Electron microscopic reconstructions show a bulge at the bottom of the C-ring whose size and shape are a close match for the hypothesized FliN tetramer. ..
  5. Chen J, Mitcheson J, Lin M, Sanguinetti M. Functional roles of charged residues in the putative voltage sensor of the HCN2 pacemaker channel. J Biol Chem. 2000;275:36465-71 pubmed
  6. Kish Trier E, Hill C. Structural biology of the proteasome. Annu Rev Biophys. 2013;42:29-49 pubmed publisher
    ..Other recent structural advances inform us about mechanisms of assembly and the role of conformational changes in the functional cycle. ..
  7. Yang W, Li C, Ward D, Kaplan J, Mansour S. Defective organellar membrane protein trafficking in Ap3b1-deficient cells. J Cell Sci. 2000;113 ( Pt 22):4077-86 pubmed
    ..In particular, the Lamp proteins were clustered on the cell surface. These findings strengthen the evidence for an alternate pathway via the plasma membrane for cargo normally transported to organelles by AP-3. ..
  8. González Bardeci N, Caramelo J, Blumenthal D, Rinaldi J, Rossi S, Moreno S. The PKA regulatory subunit from yeast forms a homotetramer: Low-resolution structure of the N-terminal oligomerization domain. J Struct Biol. 2016;193:141-54 pubmed publisher
    ..These findings indicate that the oligomerization states of PKA R subunits is more diverse than previously thought, and suggest that this might allow some forms of PKA to interact with a wide range of intracellular partners. ..
  9. Bestwick M, Jeong M, Khalimonchuk O, Kim H, Winge D. Analysis of Leigh syndrome mutations in the yeast SURF1 homolog reveals a new member of the cytochrome oxidase assembly factor family. Mol Cell Biol. 2010;30:4480-91 pubmed publisher
    ..Cyc1 is known to be important at an ill-defined step in the assembly and/or stability of CcO. This new link to Coa4 may begin to further elucidate the role of Cyc1 in CcO biogenesis. ..
  10. Buczek P, Orr R, Pyper S, Shum M, Kimmel E, Ota I, et al. Binding linkage in a telomere DNA-protein complex at the ends of Oxytricha nova chromosomes. J Mol Biol. 2005;350:938-52 pubmed
  11. Lowder B, Duyvesteyn M, Blair D. FliG subunit arrangement in the flagellar rotor probed by targeted cross-linking. J Bacteriol. 2005;187:5640-7 pubmed
    ..On the basis of the cross-linking results and the data available from mutational and electron microscopic studies, we propose a model for the organization of FliG subunits in the flagellum. ..
  12. Aldiri I, Vetter M. Characterization of the expression pattern of the PRC2 core subunit Suz12 during embryonic development of Xenopus laevis. Dev Dyn. 2009;238:3185-92 pubmed publisher
  13. Studdert C, Parkinson J. Crosslinking snapshots of bacterial chemoreceptor squads. Proc Natl Acad Sci U S A. 2004;101:2117-22 pubmed
    ..Crosslinking in receptor trimer contact mutants correlated with their signaling behaviors, suggesting that trimers of dimers are both structural and functional precursors of chemoreceptor signaling teams in bacteria. ..
  14. Parnell K, Bass B. Functional redundancy of yeast proteins Reh1 and Rei1 in cytoplasmic 60S subunit maturation. Mol Cell Biol. 2009;29:4014-23 pubmed publisher
    ..We observe that strains lacking both Reh1 and Rei1 accumulate salt-labile 60S subunits, suggesting that Reh1/Rei1 is necessary for the cytoplasmic 60S subunit to adopt its mature, stable form. ..
  15. Hone A, Meyer E, McIntyre M, McIntosh J. Nicotinic acetylcholine receptors in dorsal root ganglion neurons include the ?6?4* subtype. FASEB J. 2012;26:917-26 pubmed publisher
    ..We conclude that ?6?4* nAChRs are among the subtypes expressed by DRG, and to our knowledge, this is the first demonstration of ?6?4* in neurons outside the CNS. ..
  16. Beg A, Ernstrom G, Nix P, Davis M, Jorgensen E. Protons act as a transmitter for muscle contraction in C. elegans. Cell. 2008;132:149-60 pubmed publisher
    ..8. The identification of the mechanisms for release and reception of proton signals establishes a highly unusual mechanism for intercellular communication. ..
  17. VanDemark A, Xin H, McCullough L, Rawlins R, Bentley S, Heroux A, et al. Structural and functional analysis of the Spt16p N-terminal domain reveals overlapping roles of yFACT subunits. J Biol Chem. 2008;283:5058-68 pubmed
    ..The docking domain of H2A is identified as an important participant in maintaining stability during yFACT-mediated nucleosome reorganization, suggesting new models for the mechanism of this activity. ..
  18. Zhang H, Huang W, Zhang H, Zhu X, Craft C, Baehr W, et al. Light-dependent redistribution of visual arrestins and transducin subunits in mice with defective phototransduction. Mol Vis. 2003;9:231-7 pubmed
    ..The light-dependent translocations of Arr and transducin subunits in opposite directions appear to be based on independent mechanisms. ..
  19. Ellison M, Haberlandt C, Gómez Casati M, Watkins M, Elgoyhen A, McIntosh J, et al. Alpha-RgIA: a novel conotoxin that specifically and potently blocks the alpha9alpha10 nAChR. Biochemistry. 2006;45:1511-7 pubmed
  20. Kojima S, Blair D. Solubilization and purification of the MotA/MotB complex of Escherichia coli. Biochemistry. 2004;43:26-34 pubmed
    ..35)S-radiolabeling showed that MotA and MotB are present in a 2:1 ratio in the complex. Purified MotA/MotB complexes should enable in vitro study of the proton-induced conformational change and other aspects of stator function. ..
  21. Gonciarz M, Whitby F, Eckert D, Kieffer C, Heroux A, Sundquist W, et al. Biochemical and structural studies of yeast Vps4 oligomerization. J Mol Biol. 2008;384:878-95 pubmed publisher
    ..Our mutational analyses demonstrate that pore loop 2 residues Arg241 and Arg251 are required for efficient HIV-1 budding, thereby supporting a role for this "arginine collar" in Vps4 function. ..
  22. Gahring L, Rogers S. Nicotinic receptor subunit alpha5 modifies assembly, up-regulation, and response to pro-inflammatory cytokines. J Biol Chem. 2010;285:26049-57 pubmed publisher
    ..These findings suggest that alpha5 is an influential modulator of alpha4+beta2 nicotinic acetylcholine receptor assembly and stabilizes their expression in response to fluctuations in external conditions. ..
  23. Shestakova A, Hanono A, Drosner S, Curtiss M, Davies B, Katzmann D, et al. Assembly of the AAA ATPase Vps4 on ESCRT-III. Mol Biol Cell. 2010;21:1059-71 pubmed publisher
    ..Vta1 in turn is predicted to cause a rearrangement of the Vps4 interactions that initiates the assembly of the active Vps4 oligomer. ..
  24. Backhaus S, Zakrzewicz A, Richter K, Damm J, Wilker S, Fuchs Moll G, et al. Surfactant inhibits ATP-induced release of interleukin-1β via nicotinic acetylcholine receptors. J Lipid Res. 2017;58:1055-1066 pubmed publisher
    ..In conclusion, the surfactant constituent, DPPC, efficiently inhibits ATP-induced inflammasome activation and maturation of IL-1β in human monocytes by a mechanism involving nAChRs. ..
  25. Dowell C, Olivera B, Garrett J, Staheli S, Watkins M, Kuryatov A, et al. Alpha-conotoxin PIA is selective for alpha6 subunit-containing nicotinic acetylcholine receptors. J Neurosci. 2003;23:8445-52 pubmed
    ..Exploiting the unusual intron conservation of conotoxin genes may represent a more general approach for defining conotoxin ligand scaffolds to discriminate among closely related receptor populations. ..
  26. McIntosh J, Plazas P, Watkins M, Gómez Casati M, Olivera B, Elgoyhen A. A novel alpha-conotoxin, PeIA, cloned from Conus pergrandis, discriminates between rat alpha9alpha10 and alpha7 nicotinic cholinergic receptors. J Biol Chem. 2005;280:30107-12 pubmed
    ..Alpha-conotoxin PeIA represents a novel probe to differentiate responses mediated either through alpha9alpha10 or alpha7 nAChRs in those tissues where both receptors are expressed. ..
  27. Formosa T, Eriksson P, Wittmeyer J, Ginn J, Yu Y, Stillman D. Spt16-Pob3 and the HMG protein Nhp6 combine to form the nucleosome-binding factor SPN. EMBO J. 2001;20:3506-17 pubmed
    ..These complexes have altered electrophoretic mobility and a distinct pattern of enhanced sensitivity to DNase I. These results suggest that Spt16-Pob3 and Nhp6 cooperate to function as a novel nucleosome reorganizing factor. ..
  28. Zhou Z, Callaway K, Weber D, Jensen P. Cutting edge: HLA-DM functions through a mechanism that does not require specific conserved hydrogen bonds in class II MHC-peptide complexes. J Immunol. 2009;183:4187-91 pubmed publisher
    ..Our results support the conclusion that no individual component of the conserved hydrogen bond network plays an essential role in the DM catalytic mechanism. ..
  29. Gordon M, Holt D, Panigrahi A, Wilhelm B, Erdjument Bromage H, Tempst P, et al. Genome-wide dynamics of SAPHIRE, an essential complex for gene activation and chromatin boundaries. Mol Cell Biol. 2007;27:4058-69 pubmed
    ..SAPHIRE localization is dynamic, as activated genes rapidly acquire SAPHIRE. Furthermore, saphire mutants dramatically shift a heterochromatin-euchromatin boundary in Chr1, suggesting a novel role in boundary regulation. ..
  30. Bajorek M, Schubert H, McCullough J, Langelier C, Eckert D, Stubblefield W, et al. Structural basis for ESCRT-III protein autoinhibition. Nat Struct Mol Biol. 2009;16:754-62 pubmed publisher
  31. McIntosh J, Absalom N, Chebib M, Elgoyhen A, Vincler M. Alpha9 nicotinic acetylcholine receptors and the treatment of pain. Biochem Pharmacol. 2009;78:693-702 pubmed publisher
    ..1 binding to or action on cloned GABA-B receptors expressed in HEK cells or Xenopus oocytes. We review the background, findings and implications of use of compounds that act on alpha9* nAChRs.(1). ..
  32. Twede V, Teichert R, Walker C, Gruszczyński P, Kaźmierkiewicz R, Bulaj G, et al. Conantokin-Br from Conus brettinghami and selectivity determinants for the NR2D subunit of the NMDA receptor. Biochemistry. 2009;48:4063-73 pubmed publisher
  33. Biswas D, Takahata S, Stillman D. Different genetic functions for the Rpd3(L) and Rpd3(S) complexes suggest competition between NuA4 and Rpd3(S). Mol Cell Biol. 2008;28:4445-58 pubmed publisher
    ..Our results suggest that the Rpd3(L) and Rpd3(S) complexes have distinct functions in vivo and that the relative amounts of the two forms alter the effectiveness of other chromatin-altering complexes, such as FACT and NuA4. ..
  34. Morita E, Sandrin V, Alam S, Eckert D, Gygi S, Sundquist W. Identification of human MVB12 proteins as ESCRT-I subunits that function in HIV budding. Cell Host Microbe. 2007;2:41-53 pubmed
    ..Taken together, these studies define the composition of human ESCRT-I complexes and indicate that the MVB12 subunits play a unique role in regulating ESCRT-mediated virus budding. ..
  35. VanDemark A, Kasten M, Ferris E, Heroux A, Hill C, Cairns B. Autoregulation of the rsc4 tandem bromodomain by gcn5 acetylation. Mol Cell. 2007;27:817-28 pubmed
    ..Additional regulatory mechanisms are indicated as H3S10 phosphorylation inhibits Rsc4 binding to H3K14ac peptides. ..
  36. Khalimonchuk O, Bird A, Winge D. Evidence for a pro-oxidant intermediate in the assembly of cytochrome oxidase. J Biol Chem. 2007;282:17442-9 pubmed
    ..Studies on the hydrogen peroxide sensitivity in CcO assembly mutants reveal new aspects of the CcO assembly process. ..
  37. Sarkar M, Paul K, Blair D. Subunit organization and reversal-associated movements in the flagellar switch of Escherichia coli. J Biol Chem. 2010;285:675-84 pubmed publisher
    ..These results are discussed in the framework of a structurally grounded hypothesis for the switching mechanism. ..
  38. Braun T, Al Mawsawi L, Kojima S, Blair D. Arrangement of core membrane segments in the MotA/MotB proton-channel complex of Escherichia coli. Biochemistry. 2004;43:35-45 pubmed
  39. Pierrel F, Khalimonchuk O, Cobine P, Bestwick M, Winge D. Coa2 is an assembly factor for yeast cytochrome c oxidase biogenesis that facilitates the maturation of Cox1. Mol Cell Biol. 2008;28:4927-39 pubmed publisher
    ..The matrix-localized Coa2 protein may aid in stabilizing an early Cox1 intermediate containing the nuclear subunits Cox5a and Cox6. ..
  40. Seebohm G, Pusch M, Chen J, Sanguinetti M. Pharmacological activation of normal and arrhythmia-associated mutant KCNQ1 potassium channels. Circ Res. 2003;93:941-7 pubmed
    ..Most KCNQ1 mutant channels responded to R-L3 similarly to wild-type channels, but one mutant channel (G306R) was insensitive to R-L3 possibly because it disrupted a key component of the drug-binding site. ..