Experts and Doctors on molecular chaperones in Salt Lake City, Utah, United States


Locale: Salt Lake City, Utah, United States
Topic: molecular chaperones

Top Publications

  1. Fetherolf M, Boyd S, Taylor A, Kim H, Wohlschlegel J, Blackburn N, et al. Copper-zinc superoxide dismutase is activated through a sulfenic acid intermediate at a copper ion entry site. J Biol Chem. 2017;292:12025-12040 pubmed publisher
    ..Sod1 is the predominant disulfide bond-requiring enzyme in the cytoplasm, and this copper-induced mechanism of disulfide bond formation obviates the need for a thiol/disulfide oxidoreductase in that compartment. ..
  2. Kulesus R, Diaz Perez K, Slechta E, Eto D, Mulvey M. Impact of the RNA chaperone Hfq on the fitness and virulence potential of uropathogenic Escherichia coli. Infect Immun. 2008;76:3019-26 pubmed publisher
    ..Cumulatively, our data indicate that Hfq likely affects UPEC virulence-related phenotypes primarily by modulating membrane homeostasis and envelope stress response pathways. ..
  3. Heaton D, Nittis T, Srinivasan C, Winge D. Mutational analysis of the mitochondrial copper metallochaperone Cox17. J Biol Chem. 2000;275:37582-7 pubmed
    ..Thus, only three cysteinyl residues are important for the ligation of three Cu(I) ions. A novel mode of Cu(I) binding is predicted. ..
  4. McCullough J, Sundquist W. Putting a finger in the ring. Nat Struct Mol Biol. 2014;21:1025-7 pubmed publisher
  5. Srinivasan C, Posewitz M, George G, Winge D. Characterization of the copper chaperone Cox17 of Saccharomyces cerevisiae. Biochemistry. 1998;37:7572-7 pubmed
    ..26 A, plus a short Cu-Cu distance of 2.7 A, indicating a binuclear cluster in Cox17. The cuprous-thiolate cluster in Cox17 is substantially more labile than structurally related clusters in metallothioneins. ..
  6. Constantine R, Zhang H, Gerstner C, Frederick J, Baehr W. Uncoordinated (UNC)119: coordinating the trafficking of myristoylated proteins. Vision Res. 2012;75:26-32 pubmed publisher
    ..These interactions establish UNC119 polypeptides as novel lipid-binding chaperones with specificity for a diverse subset of myristoylated proteins. ..
  7. Rigby K, Cobine P, Khalimonchuk O, Winge D. Mapping the functional interaction of Sco1 and Cox2 in cytochrome oxidase biogenesis. J Biol Chem. 2008;283:15015-22 pubmed publisher
    ..In addition, the mutants failed to suppress the hydrogen peroxide sensitivity of sco1Delta cells. These studies implicate different surfaces on Sco1 for interaction or function with Cox17 and Cox2. ..
  8. Cobine P, Pierrel F, Bestwick M, Winge D. Mitochondrial matrix copper complex used in metallation of cytochrome oxidase and superoxide dismutase. J Biol Chem. 2006;281:36552-9 pubmed
    ..These data suggest that attenuation of the matrix CuL complex via heterologous competitors limits available copper for metallation of CcO and Sod1 within the IMS. The ligand also exists in the cytoplasm in an apparent metal-free state. ..
  9. Davis Kaplan S, Compton M, Flannery A, Ward D, Kaplan J, Stevens T, et al. PKR1 encodes an assembly factor for the yeast V-type ATPase. J Biol Chem. 2006;281:32025-35 pubmed
    ..Our data indicate that Pkr1p functions together with the other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector. ..

More Information


  1. Horng Y, Cobine P, Maxfield A, Carr H, Winge D. Specific copper transfer from the Cox17 metallochaperone to both Sco1 and Cox11 in the assembly of yeast cytochrome C oxidase. J Biol Chem. 2004;279:35334-40 pubmed
    ..Metallation of these domains was strictly dependent on the co-expression of Cox17. Thus, Cox17 represents a novel copper chaperone that delivers copper to two proteins. ..
  2. Raina S, Missiakas D, Baird L, Kumar S, Georgopoulos C. Identification and transcriptional analysis of the Escherichia coli htrE operon which is homologous to pap and related pilin operons. J Bacteriol. 1993;175:5009-21 pubmed
  3. Rigby K, Zhang L, Cobine P, George G, Winge D. characterization of the cytochrome c oxidase assembly factor Cox19 of Saccharomyces cerevisiae. J Biol Chem. 2007;282:10233-42 pubmed
    ..Cysteinyl residues important for Cu(I) binding are also shown to be important for the in vivo function of Cox19. Thus, a correlation exists in the ability to bind Cu(I) and in vivo function. ..
  4. Takahata S, Yu Y, Stillman D. FACT and Asf1 regulate nucleosome dynamics and coactivator binding at the HO promoter. Mol Cell. 2009;34:405-15 pubmed publisher
    ..The Swi/Snf, SAGA, and Mediator coactivators bind first to the far upstream promoter region and subsequently to a promoter proximal region, and FACT and Asf1 are both required for this coactivator re-recruitment. ..
  5. Cui T, Smith P, Fox J, Khalimonchuk O, Winge D. Late-stage maturation of the Rieske Fe/S protein: Mzm1 stabilizes Rip1 but does not facilitate its translocation by the AAA ATPase Bcs1. Mol Cell Biol. 2012;32:4400-9 pubmed publisher
    ..However, the Rip1 variants were not stably associated with the supercomplex. The induced supercomplex stabilization by the Rip1 N terminus was independent of Mzm1. ..
  6. Kish Trier E, Hill C. Structural biology of the proteasome. Annu Rev Biophys. 2013;42:29-49 pubmed publisher
    ..Other recent structural advances inform us about mechanisms of assembly and the role of conformational changes in the functional cycle. ..
  7. Kemble D, Whitby F, Robinson H, McCullough L, Formosa T, Hill C. Structure of the Spt16 middle domain reveals functional features of the histone chaperone FACT. J Biol Chem. 2013;288:10188-94 pubmed publisher
    ..Our structure provides a framework for interpreting a large body of genetic data regarding the physiological functions of FACT, including the identification of potential interaction surfaces for binding histones or other proteins. ..
  8. Heaton D, George G, Garrison G, Winge D. The mitochondrial copper metallochaperone Cox17 exists as an oligomeric, polycopper complex. Biochemistry. 2001;40:743-51 pubmed
    ..Thus, the oligomeric state of Cox17 may be important to its physiological function. ..
  9. Aung T, Ozaki M, Lee M, Schlötzer Schrehardt U, Thorleifsson G, Mizoguchi T, et al. Genetic association study of exfoliation syndrome identifies a protective rare variant at LOXL1 and five new susceptibility loci. Nat Genet. 2017;49:993-1004 pubmed publisher
    ..3 (TMEM136), 6p21 (AGPAT1), 3p24 (RBMS3) and 5q23 (near SEMA6A). These findings provide biological insights into the pathology of XFS and highlight a potential role for naturally occurring rare LOXL1 variants in disease biology. ..
  10. Christensen C, Gorsich S, Brown R, Jones L, Brown J, Shaw J, et al. Mitochondrial GFA2 is required for synergid cell death in Arabidopsis. Plant Cell. 2002;14:2215-32 pubmed
    ..GFA2 is targeted to mitochondria in Arabidopsis and partially complements a yeast mdj1 mutant, suggesting that GFA2 is the Arabidopsis ortholog of yeast Mdj1p. These data suggest a role for mitochondria in cell death in plants. ..