Genomes and Genes
Experts and Doctors on protein binding in Dallas, Texas, United States
Locale: Dallas, Texas, United States
Topic: protein binding
Publications330 found, 100 shown here
- Ross J, Chen Y, Muller J, Barylko B, Wang L, Banks H, et al. Dimeric endophilin A2 stimulates assembly and GTPase activity of dynamin 2. Biophys J. 2011;100:729-737 pubmed publisher..These findings support the view that endophilin-dynamin interactions play an important role in endocytosis. ..
- Liu Z, Kraus W. Catalytic-Independent Functions of PARP-1 Determine Sox2 Pioneer Activity at Intractable Genomic Loci. Mol Cell. 2017;65:589-603.e9 pubmed publisher..The conditional pioneer activity observed with Sox2 at a subset of binding sites may be a key feature of other pioneer TFs operating at intractable genomic loci. ..
- Biederer T, Sudhof T. Mints as adaptors. Direct binding to neurexins and recruitment of munc18. J Biol Chem. 2000;275:39803-6 pubmed..Our data support a model whereby one of the functions of Mints is to localize the vesicle fusion protein Munc18 to those sites at the plasma membrane that are defined by neurexins, presumably in the vicinity of points of exocytosis. ..
- Ullrich B, Li C, Zhang J, McMahon H, Anderson R, Geppert M, et al. Functional properties of multiple synaptotagmins in brain. Neuron. 1994;13:1281-91 pubmed
- Wang L, Ding L, Jones C, Jones R. Drosophila Enhancer of zeste protein interacts with dSAP18. Gene. 2002;285:119-25 pubmed..These studies provide support for a model in which dSAP18 contributes to the activities of multiple protein complexes, and potentially may mediate interactions between distinct proteins and/or protein complexes. ..
- Kanayama A, Seth R, Sun L, Ea C, Hong M, Shaito A, et al. TAB2 and TAB3 activate the NF-kappaB pathway through binding to polyubiquitin chains. Mol Cell. 2004;15:535-48 pubmed..These results indicate that polyubiquitin binding domains represent a new class of signaling domains that regulate protein kinase activity through a nonproteolytic mechanism. ..
- Haas D, White S, Lutz L, Rasar M, Hammes S. The modulator of nongenomic actions of the estrogen receptor (MNAR) regulates transcription-independent androgen receptor-mediated signaling: evidence that MNAR participates in G protein-regulated meiosis in Xenopus laevis oocytes. Mol Endocrinol. 2005;19:2035-46 pubmed..Androgen binding to AR might then release this inhibition, allowing maturation to occur. Thus, MNAR may augment multiple nongenomic signals, depending upon the context and cell type in which it is expressed. ..
- Kato M, Wynn R, Chuang J, Brautigam C, Custorio M, Chuang D. A synchronized substrate-gating mechanism revealed by cubic-core structure of the bovine branched-chain alpha-ketoacid dehydrogenase complex. EMBO J. 2006;25:5983-94 pubmed..We suggest that this gating mechanism synchronizes the binding of the two substrates to the active-site channel, which serves as a feed-forward switch to coordinate the E2b-catalyzed acyltransfer reaction. ..
- Akella R, Moon T, Goldsmith E. Unique MAP Kinase binding sites. Biochim Biophys Acta. 2008;1784:48-55 pubmed..Crystallographic evidence of these latter two binding sites is presented. ..
- Archer C, Burdine L, Liu B, Ferdous A, Johnston S, Kodadek T. Physical and functional interactions of monoubiquitylated transactivators with the proteasome. J Biol Chem. 2008;283:21789-98 pubmed publisher..A model is proposed in which activator monoubiquitylation serves to limit the lifetime of the activator-ATPase complex interaction and thus the ability of the ATPases to unfold the activator and dissociate the protein-DNA complex. ..
- Chen Z, Singer W, Danesh S, Sternweis P, Sprang S. Recognition of the activated states of Galpha13 by the rgRGS domain of PDZRhoGEF. Structure. 2008;16:1532-43 pubmed publisher..Replacement of key residues in this motif with their counterparts in p115RhoGEF confers GAP activity. ..
- Stastny P, Ring S, Lu C, Arenas J, Han M, Lavingia B. Role of immunoglobulin (Ig)-G and IgM antibodies against donor human leukocyte antigens in organ transplant recipients. Hum Immunol. 2009;70:600-4 pubmed publisher..In addition, IgM antibodies against donor HLA appeared to be associated with decreased survival of kidney and heart allografts. ..
- Jia D, Gomez T, Metlagel Z, Umetani J, Otwinowski Z, Rosen M, et al. WASH and WAVE actin regulators of the Wiskott-Aldrich syndrome protein (WASP) family are controlled by analogous structurally related complexes. Proc Natl Acad Sci U S A. 2010;107:10442-7 pubmed publisher..Strumpellin is mutated in the human disease hereditary spastic paraplegia, and its link to WASH suggests that misregulation of actin dynamics on endosomes may play a role in this disorder. ..
- Wei W, Schwaid A, Wang X, Wang X, Chen S, Chu Q, et al. Ligand Activation of ERRÎ± by Cholesterol Mediates Statin and Bisphosphonate Effects. Cell Metab. 2016;23:479-91 pubmed publisher..These findings reveal a key step in ERRÎ± regulation and explain the actions of two highly prescribed drugs, statins and bisphosphonates. ..
- Kitchens R, Munford R. CD14-dependent internalization of bacterial lipopolysaccharide (LPS) is strongly influenced by LPS aggregation but not by cellular responses to LPS. J Immunol. 1998;160:1920-8 pubmed..In these cells, LPS is thus internalized by a constitutive cellular mechanism(s) with kinetics that depend importantly upon the physical state in which the LPS is presented to the cell. ..
- Huang N, Chelliah Y, Shan Y, Taylor C, Yoo S, PARTCH C, et al. Crystal structure of the heterodimeric CLOCK:BMAL1 transcriptional activator complex. Science. 2012;337:189-94 pubmed publisher..The structure of the CLOCK:BMAL1 complex is a starting point for understanding at an atomic level the mechanism driving the mammalian circadian clock. ..
- Jiang X, Kinch L, Brautigam C, Chen X, Du F, Grishin N, et al. Ubiquitin-induced oligomerization of the RNA sensors RIG-I and MDA5 activates antiviral innate immune response. Immunity. 2012;36:959-73 pubmed publisher..These results suggest a unified mechanism of RIG-I and MDA5 activation and reveal a unique mechanism by which ubiquitin regulates cell signaling and immune response. ..
- Ruiz J, Walker S, Anderson S, Eisenstein R, Bruick R. F-box and leucine-rich repeat protein 5 (FBXL5) is required for maintenance of cellular and systemic iron homeostasis. J Biol Chem. 2013;288:552-60 pubmed publisher..These results confirm the role of FBXL5 in the in vivo maintenance of cellular and systemic iron homeostasis and reveal a privileged role for the intestine in their regulation by virtue of its unique FBXL5 iron sensitivity. ..
- Chen J, Chen Z. Regulation of NF-?B by ubiquitination. Curr Opin Immunol. 2013;25:4-12 pubmed publisher..In this review, we will focus on recent advances in understanding the role of ubiquitination in NF-?B regulation in various pathways. ..
- Tso S, Qi X, Gui W, Chuang J, Morlock L, Wallace A, et al. Structure-based design and mechanisms of allosteric inhibitors for mitochondrial branched-chain ?-ketoacid dehydrogenase kinase. Proc Natl Acad Sci U S A. 2013;110:9728-33 pubmed publisher..The results buttress the concept of targeting mitochondrial BDK as a pharmacological approach to mitigate BCAA accumulation in metabolic diseases and heart failure. ..
- Dessauer C, Tesmer J, Sprang S, Gilman A. Identification of a Gialpha binding site on type V adenylyl cyclase. J Biol Chem. 1998;273:25831-9 pubmed..The pseudosymmetrical structure of adenylyl cyclase permits bidirectional regulation of activity by homologous G protein alpha subunits. ..
- Zhou J, Scholes J, Hsieh J. Characterization of a novel negative regulator (DOC-2/DAB2) of c-Src in normal prostatic epithelium and cancer. J Biol Chem. 2003;278:6936-41 pubmed..It appears that DOC-2/DAB2 can bind to Src homology 3 domain of c-Src and maintain it in an inactive conformation. Thus, this study provides a new mechanism for modulating c-Src in prostatic epithelium and cancer. ..
- Ran S, Huang X, Downes A, Thorpe P. Evaluation of novel antimouse VEGFR2 antibodies as potential antiangiogenic or vascular targeting agents for tumor therapy. Neoplasia. 2003;5:297-307 pubmed publisher..Vascular damage was not observed in normal organs, including kidneys and pancreas. These studies demonstrate that anti-VEGFR2 antibodies have potential for vascular targeting and imaging of tumors in vivo. ..
- Matos M, Mukherjee K, Chen X, Rizo J, Sudhof T. Evidence for SNARE zippering during Ca2+-triggered exocytosis in PC12 cells. Neuropharmacology. 2003;45:777-86 pubmed
- Kinch L, Cheek S, Grishin N. EDD, a novel phosphotransferase domain common to mannose transporter EIIA, dihydroxyacetone kinase, and DegV. Protein Sci. 2005;14:360-7 pubmed
- Widlak P, Kalinowska M, Parseghian M, Lu X, Hansen J, Garrard W. The histone H1 C-terminal domain binds to the apoptotic nuclease, DNA fragmentation factor (DFF40/CAD) and stimulates DNA cleavage. Biochemistry. 2005;44:7871-8 pubmed..We conclude that the interactions identified here between the histone H1 CTD and DFF40/CAD target and activate linker DNA cleavage during the terminal stages of apoptosis. ..
- Xu D, Farmer A, Chook Y. Recognition of nuclear targeting signals by Karyopherin-? proteins. Curr Opin Struct Biol. 2010;20:782-90 pubmed publisher..We review recent findings relating to how these three classes of nuclear targeting signals are recognized by their Karyopherin-? nuclear transport factors. ..
- Groppe J, Wu J, Shore E, Kaplan F. In vitro analyses of the dysregulated R206H ALK2 kinase-FKBP12 interaction associated with heterotopic ossification in FOP. Cells Tissues Organs. 2011;194:291-5 pubmed publisher..In conclusion, substitution with histidine leads to partial loss of inhibition of the mutant type I receptor through diminished binding of FKBP12, which may act as a gradient reader in morphogenetic contexts...
- Raingo J, Khvotchev M, Liu P, Darios F, Li Y, Ramirez D, et al. VAMP4 directs synaptic vesicles to a pool that selectively maintains asynchronous neurotransmission. Nat Neurosci. 2012;15:738-45 pubmed publisher..These results provide molecular insight into how synapses diversify their release properties by taking advantage of distinct synaptic vesicle-associated SNAREs...
- He X, Kuo Y, Rosche T, Zhang X. Structural basis for autoinhibition of the guanine nucleotide exchange factor FARP2. Structure. 2013;21:355-64 pubmed publisher..This conformation is stabilized by multiple interactions among the domains and two well-structured inter-domain linkers. Our cell-based activity assays confirm the suppression of the FARP2 GEF activity by these auto-inhibitory elements. ..
- Mitoma H, Hanabuchi S, Kim T, Bao M, Zhang Z, Sugimoto N, et al. The DHX33 RNA helicase senses cytosolic RNA and activates the NLRP3 inflammasome. Immunity. 2013;39:123-35 pubmed publisher..DHX33 interacted with NLRP3 and formed the inflammasome complex following stimulation with RNA. We therefore identified DHX33 as a cytosolic RNA sensor that activates the NLRP3 inflammasome. ..
- McMahon H, Sudhof T. Synaptic core complex of synaptobrevin, syntaxin, and SNAP25 forms high affinity alpha-SNAP binding site. J Biol Chem. 1995;270:2213-7 pubmed..Thus, alpha-SNAP probably functions in a late step of the membrane fusion reaction after the formation of the synaptobrevin-syntaxin-SNAP25 core complex. ..
- Okamoto M, Sudhof T. Mints, Munc18-interacting proteins in synaptic vesicle exocytosis. J Biol Chem. 1997;272:31459-64 pubmed..F. J. (1995) Nature 374, 173-177). Our data suggest a model whereby local production of phosphatidylinositol phosphates may trigger the binding of vesicles to the active zone via the Mint.Munc18-1 complex in conjunction with syntaxin 1. ..
- Sakai J, Nohturfft A, Goldstein J, Brown M. Cleavage of sterol regulatory element-binding proteins (SREBPs) at site-1 requires interaction with SREBP cleavage-activating protein. Evidence from in vivo competition studies. J Biol Chem. 1998;273:5785-93 pubmed..We interpret these data to indicate that the SREBP.SCAP complex directs the Site-1 protease to its target in the lumenal domain of SREBP and that disruption of this complex inactivates the Site-1 cleavage reaction...
- Hosaka M, Sudhof T. Synapsin III, a novel synapsin with an unusual regulation by Ca2+. J Biol Chem. 1998;273:13371-4 pubmed..Thus vertebrates express three distinct synapsins that utilize ATP but are specialized for different modes of direct Ca2+ regulation in synaptic function. ..
- Dent J, Smith M, Vassilatis D, Avery L. The genetics of ivermectin resistance in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2000;97:2674-9 pubmed..Our results suggest that the evolution of drug resistance can be slowed by targeting antibiotic drugs to several members of a multigene family. ..
- Gupta M, Geller D, Auchus R. Pitfalls in characterizing P450c17 mutations associated with isolated 17,20-lyase deficiency. J Clin Endocrinol Metab. 2001;86:4416-23 pubmed..Mutations R347H and R358Q are the only two such mutations found in humans proven to cause isolated 17,20-lyase deficiency. ..
- Zhou J, Johnson J, Ghetie V, Ober R, Ward E. Generation of mutated variants of the human form of the MHC class I-related receptor, FcRn, with increased affinity for mouse immunoglobulin G. J Mol Biol. 2003;332:901-13 pubmed..In turn, understanding human FcRn function has significance for the optimization of the serum half-lives of therapeutic and prophylactic antibodies. ..
- Gocke C, Yu H, Kang J. Systematic identification and analysis of mammalian small ubiquitin-like modifier substrates. J Biol Chem. 2005;280:5004-12 pubmed..Therefore, sumoylation appears to regulate the functions of its substrates through multiple, context-dependent mechanisms. ..
- Sinha S, Wetterer M, Sprang S, Schultz J, Linder J. Origin of asymmetry in adenylyl cyclases: structures of Mycobacterium tuberculosis Rv1900c. EMBO J. 2005;24:663-73 pubmed..Such a mechanism of half-of-sites-reactivity suggests that mammalian heterodimeric adenylyl cyclases may have evolved from gene duplication of a primitive prokaryote-type cyclase, followed by loss of function in one active site. ..
- Maximov A, Shin O, Liu X, Sudhof T. Synaptotagmin-12, a synaptic vesicle phosphoprotein that modulates spontaneous neurotransmitter release. J Cell Biol. 2007;176:113-24 pubmed..Our data suggest that spontaneous synaptic-vesicle exocytosis is selectively modulated by a Ca(2+)-independent synaptotagmin isoform, synaptotagmin-12, which is controlled by cAMP-dependent phosphorylation. ..
- Potthoff M, Wu H, Arnold M, Shelton J, Backs J, McAnally J, et al. Histone deacetylase degradation and MEF2 activation promote the formation of slow-twitch myofibers. J Clin Invest. 2007;117:2459-67 pubmed..These findings provide what we believe are new insights into the molecular basis of skeletal muscle function and have important implications for possible therapeutic interventions into muscular diseases. ..
- Nishi Y, Rogers E, Robertson S, Lin R. Polo kinases regulate C. elegans embryonic polarity via binding to DYRK2-primed MEX-5 and MEX-6. Development. 2008;135:687-97 pubmed publisher..Our results provide a mechanism by which MEX-5 and MEX-6 function is temporally regulated during the crucial oocyte-to-embryo transition. ..
- Phillips M, Gujjar R, Malmquist N, White J, El Mazouni F, Baldwin J, et al. Triazolopyrimidine-based dihydroorotate dehydrogenase inhibitors with potent and selective activity against the malaria parasite Plasmodium falciparum. J Med Chem. 2008;51:3649-53 pubmed publisher..This study has identified the first nanomolar PfDHODH inhibitor with potent antimalarial activity in whole cells (EC50 = 79 nM). ..
- Zhao Z, Michaely P. The epidermal growth factor homology domain of the LDL receptor drives lipoprotein release through an allosteric mechanism involving H190, H562, and H586. J Biol Chem. 2008;283:26528-37 pubmed publisher..We propose that H190, H562, and H586 are part of an allosteric mechanism that drives lipoprotein release. ..
- Gattineni J, Baum M. Regulation of phosphate transport by fibroblast growth factor 23 (FGF23): implications for disorders of phosphate metabolism. Pediatr Nephrol. 2010;25:591-601 pubmed publisher..Here, we also discuss new potentially clinically important data pointing to the receptor(s) that mediate the binding and action of FGF23 and Klotho. ..
- Tang W, Li Y, Osimiri L, Zhang C. Osteoblast-specific transcription factor Osterix (Osx) is an upstream regulator of Satb2 during bone formation. J Biol Chem. 2011;286:32995-3002 pubmed publisher..Taken together, our findings indicate that Osx is an upstream regulator of Satb2 during bone formation. This reveals a new additional link of the transcriptional regulation mechanism that Osx controls bone formation. ..
- Straud S, Lee I, Song B, Avery L, You Y. The jaw of the worm: GTPase-activating protein EAT-17 regulates grinder formation in Caenorhabditis elegans. Genetics. 2013;195:115-25 pubmed publisher..2. Based on the conserved function of Rab6 in vesicular transport, we propose that EAT-17 regulates the turnover rate of RAB-6.2 activity in cargo trafficking for grinder formation. ..
- Shi G, Xie P, Qu Z, Zhang Z, Dong Z, An Y, et al. Distinct Roles of HDAC3 in the Core Circadian Negative Feedback Loop Are Critical for Clock Function. Cell Rep. 2016;14:823-834 pubmed publisher..Therefore, these two opposing but temporally separated roles of HDAC3 in the negative feedback loop provide a mechanism for robust circadian gene expression. ..
- Wadman I, Li J, Bash R, Forster A, Osada H, Rabbitts T, et al. Specific in vivo association between the bHLH and LIM proteins implicated in human T cell leukemia. EMBO J. 1994;13:4831-9 pubmed..Thus, the activated alleles of these genes may promote leukemia cooperatively, perhaps as a result of bHLH-LIM interactions between their protein products. ..
- Zhao M, Li X, Chen Z. CC2D1A, a DM14 and C2 domain protein, activates NF-kappaB through the canonical pathway. J Biol Chem. 2010;285:24372-80 pubmed publisher..In addition, the deubiquitination enzyme Cylindromatosis (CYLD) negatively regulates the activity of CC2D1A. These results suggest that CC2D1A activates NF-kappaB through the canonical IKK pathway. ..
- Kaur K, Ruben L. Protein translation elongation factor-1 alpha from Trypanosoma brucei binds calmodulin. J Biol Chem. 1994;269:23045-50 pubmed..Biotinylated CaM bound weakly to trypanosome and rabbit EF-1 alpha in a gel overlay assay. This report demonstrates the direct interaction between CaM and the translation elongation factor EF-1 alpha...
- Hsu H, Wadman I, Baer R. Formation of in vivo complexes between the TAL1 and E2A polypeptides of leukemic T cells. Proc Natl Acad Sci U S A. 1994;91:3181-5 pubmed..Thus, the malignant properties of TAL1 may be due to obligate interaction with the E2A polypeptides. ..
- Huang W, Feaver W, Tomkinson A, Friedberg E. The N-degron protein degradation strategy for investigating the function of essential genes: requirement for replication protein A and proliferating cell nuclear antigen proteins for nucleotide excision repair in yeast extracts. Mutat Res. 1998;408:183-94 pubmed..We also demonstrate a physical interaction between RPA1 protein and subunits of the RNA polymerase II basal transcription factor IIH (TFIIH). ..
- Yang Q, Kong Y, Rothermel B, Garry D, Bassel Duby R, Williams R. The winged-helix/forkhead protein myocyte nuclear factor beta (MNF-beta) forms a co-repressor complex with mammalian sin3B. Biochem J. 2000;345 Pt 2:335-43 pubmed..Transcriptional repression by MNF-beta-mSin3 complexes may contribute to the co-ordination of cellular proliferation and terminal differentiation of myogenic precursor cells. ..
- Wang D, Chang P, Wang Z, Sutherland L, Richardson J, Small E, et al. Activation of cardiac gene expression by myocardin, a transcriptional cofactor for serum response factor. Cell. 2001;105:851-62 pubmed..Myocardin is the founding member of a class of muscle transcription factors and provides a mechanism whereby SRF can convey myogenic activity to cardiac muscle genes. ..
- Cao X, Sudhof T. A transcriptionally [correction of transcriptively] active complex of APP with Fe65 and histone acetyltransferase Tip60. Science. 2001;293:115-20 pubmed..This complex potently stimulates transcription via heterologous Gal4- or LexA-DNA binding domains, suggesting that release of the cytoplasmic tail of APP by gamma-cleavage may function in gene expression. ..
- Jeong C, Yang S, Xie Y, Zhang L, Johnston S, Kodadek T. Evidence that Gal11 protein is a target of the Gal4 activation domain in the mediator. Biochemistry. 2001;40:9421-7 pubmed
- Charite J, McFadden D, Merlo G, Levi G, Clouthier D, Yanagisawa M, et al. Role of Dlx6 in regulation of an endothelin-1-dependent, dHAND branchial arch enhancer. Genes Dev. 2001;15:3039-49 pubmed..These results suggest that Dlx6 acts as an intermediary between ET-1 signaling and dHAND transcription during craniofacial morphogenesis. ..
- Yang T, Espenshade P, Wright M, Yabe D, Gong Y, Aebersold R, et al. Crucial step in cholesterol homeostasis: sterols promote binding of SCAP to INSIG-1, a membrane protein that facilitates retention of SREBPs in ER. Cell. 2002;110:489-500 pubmed..Mutant SCAP(Y298C) fails to bind INSIG-1 and is resistant to sterol-mediated inhibition of ER exit. By facilitating sterol-dependent ER retention of SCAP, INSIG-1 plays a central role in cholesterol homeostasis. ..
- Gallagher E, Xu S, Moomaw C, Slaughter C, Cobb M. Binding of JNK/SAPK to MEKK1 is regulated by phosphorylation. J Biol Chem. 2002;277:45785-92 pubmed..Phosphorylation of this site inhibits binding of JNK/SAPK to MEKK1. Thus, we propose a mechanism by which the MEKK1-dependent JNK/SAPK pathway is negatively regulated by PAK through phosphorylation of serine 67. ..
- Dulubova I, Yamaguchi T, Arac D, Li H, Huryeva I, Min S, et al. Convergence and divergence in the mechanism of SNARE binding by Sec1/Munc18-like proteins. Proc Natl Acad Sci U S A. 2003;100:32-7 pubmed
- Hiesberger T, Shao X, Gourley E, Reimann A, Pontoglio M, Igarashi P. Role of the hepatocyte nuclear factor-1beta (HNF-1beta) C-terminal domain in Pkhd1 (ARPKD) gene transcription and renal cystogenesis. J Biol Chem. 2005;280:10578-86 pubmed..Expression of HNF-1alpha in proximal tubules may protect against cystogenesis. ..
- Yang M, Gocke C, Luo X, Borek D, Tomchick D, Machius M, et al. Structural basis for CoREST-dependent demethylation of nucleosomes by the human LSD1 histone demethylase. Mol Cell. 2006;23:377-87 pubmed..This spatially separated, multivalent nucleosome binding mode may apply to other chromatin-modifying enzymes that generally contain multiple nucleosome binding modules. ..
- Sato K, Yang X, Yudate T, Chung J, Wu J, Luby Phelps K, et al. Dectin-2 is a pattern recognition receptor for fungi that couples with the Fc receptor gamma chain to induce innate immune responses. J Biol Chem. 2006;281:38854-66 pubmed..We conclude that dectin-2 is a PRR for fungi that employs signaling through FcRgamma to induce innate immune responses. ..
- Dulubova I, Khvotchev M, Liu S, Huryeva I, Sudhof T, Rizo J. Munc18-1 binds directly to the neuronal SNARE complex. Proc Natl Acad Sci U S A. 2007;104:2697-702 pubmed
- Wang J, Zhang D, Lei Y, Xu F, Cohen J, Hobbs H, et al. Purification and reconstitution of sterol transfer by native mouse ABCG5 and ABCG8. Biochemistry. 2008;47:5194-204 pubmed publisher..Both G5 and G8 have short but highly conserved cytoplasmic tails. The functional roles of these C-terminal regions were examined using an in vivo functional assay...
- Regmi S, Rothberg K, Hubbard J, Ruben L. The RACK1 signal anchor protein from Trypanosoma brucei associates with eukaryotic elongation factor 1A: a role for translational control in cytokinesis. Mol Microbiol. 2008;70:724-45 pubmed publisher..The synergy between anisomycin and TbRACK1 RNAi suggests that continued translation is required for complete ingression of the cleavage furrow...
- Deford Watts L, Dougall D, Belkaya S, Johnson B, Eitson J, Roybal K, et al. The CD3 zeta subunit contains a phosphoinositide-binding motif that is required for the stable accumulation of TCR-CD3 complex at the immunological synapse. J Immunol. 2011;186:6839-47 pubmed publisher..Such findings demonstrate a novel functional role for CD3 ? BRS-phosphoinositide interactions in supporting T cell activation. ..
- Kadamur G, Ross E. Intrinsic Pleckstrin Homology (PH) Domain Motion in Phospholipase C-? Exposes a G?? Protein Binding Site. J Biol Chem. 2016;291:11394-406 pubmed publisher..Therefore, intrinsic movement of the PH domain in PLC-? modulates G?? access to its binding site. ..
- Zhang Y, Lee K, Kinch L, Clark L, Grishin N, Rosenbaum D, et al. Direct Demonstration That Loop1 of Scap Binds to Loop7: A CRUCIAL EVENT IN CHOLESTEROL HOMEOSTASIS. J Biol Chem. 2016;291:12888-96 pubmed publisher..Point mutations that disrupt the Loop1-Loop7 interaction prevented secretion of the Loop1-Loop7 fusion protein. These data provide direct documentation of intramolecular Loop1-Loop7 binding, a central event in cholesterol homeostasis. ..
- Herz J, Goldstein J, Strickland D, Ho Y, Brown M. 39-kDa protein modulates binding of ligands to low density lipoprotein receptor-related protein/alpha 2-macroglobulin receptor. J Biol Chem. 1991;266:21232-8 pubmed..A potential in vivo role of the 39-kDa protein is to modulate the uptake of apoE-enriched lipoproteins and activated alpha 2-macroglobulin in hepatic and extrahepatic tissues. ..
- Ichtchenko K, Nguyen T, Sudhof T. Structures, alternative splicing, and neurexin binding of multiple neuroligins. J Biol Chem. 1996;271:2676-82 pubmed..Thus, neuroligins constitute a multigene family of brain-specific proteins with distinct isoforms that may have overlapping functions in mediating recognition processes between neurons. ..
- Russell S, Sathyanarayana U, Johnston S. Isolation and characterization of SUG2. A novel ATPase family component of the yeast 26 S proteasome. J Biol Chem. 1996;271:32810-7 pubmed..With its highly conserved mammalian homologs, human p42 and ground squirrel CADp44, SUG2 defines a new class of proteasomal CAD proteins. ..
- Jones C, Ng J, Peterson A, Morgan K, Simon J, Jones R. The Drosophila esc and E(z) proteins are direct partners in polycomb group-mediated repression. Mol Cell Biol. 1998;18:2825-34 pubmed..In light of these earlier observations and the molecular data presented here, we discuss how esc-E(z) protein complexes may contribute to transcriptional silencing by the Pc-G. ..
- Shepard J, Reick M, Olson S, Graveley B. Characterization of U2AF(6), a splicing factor related to U2AF(35). Mol Cell Biol. 2002;22:221-30 pubmed..Based on its sequence and functional similarity to U2AF(35), U2AF(26) may play a role in regulating alternative splicing. ..
- Fernandez Chacon R, Shin O, Königstorfer A, Matos M, Meyer A, Garcia J, et al. Structure/function analysis of Ca2+ binding to the C2A domain of synaptotagmin 1. J Neurosci. 2002;22:8438-46 pubmed..Our data also demonstrate that subtle changes in the biochemical properties of synaptotagmin 1 can result in significant alterations in synaptic responses. ..
- Sever N, Yang T, Brown M, Goldstein J, DeBose Boyd R. Accelerated degradation of HMG CoA reductase mediated by binding of insig-1 to its sterol-sensing domain. Mol Cell. 2003;11:25-33 pubmed..Insig-1 appears to play an essential role in the sterol-mediated trafficking of two proteins with sterol-sensing domains, HMG CoA reductase and SCAP. ..
- Singh S, Yang K, Karthikeyan S, Huynh T, Zhang X, Phillips M, et al. The thrH gene product of Pseudomonas aeruginosa is a dual activity enzyme with a novel phosphoserine:homoserine phosphotransferase activity. J Biol Chem. 2004;279:13166-73 pubmed
- Zhang Q, Zhang L, Wang B, Ou C, Chien C, Jiang J. A hedgehog-induced BTB protein modulates hedgehog signaling by degrading Ci/Gli transcription factor. Dev Cell. 2006;10:719-29 pubmed..We provide evidence that HIB prevents aberrant Hh signaling posterior to the morphogenic furrow, which is essential for normal eye development. ..
- Janowski B, Huffman K, Schwartz J, Ram R, Nordsell R, Shames D, et al. Involvement of AGO1 and AGO2 in mammalian transcriptional silencing. Nat Struct Mol Biol. 2006;13:787-92 pubmed..Our data indicate key linkages and important mechanistic distinctions between transcriptional and post-transcriptional silencing pathways in mammalian cells. ..
- Seth A, Otomo C, Rosen M. Autoinhibition regulates cellular localization and actin assembly activity of the diaphanous-related formins FRLalpha and mDia1. J Cell Biol. 2006;174:701-13 pubmed..These results suggest that mutual autoinhibition of biochemical activity and cellular localization may be a general regulatory principle for DRFs and demonstrate a novel role for formins in immune function. ..
- Szebenyi G, Hall B, Yu R, Hashim A, KRAMER H. Hook2 localizes to the centrosome, binds directly to centriolin/CEP110 and contributes to centrosomal function. Traffic. 2007;8:32-46 pubmed..Thus, we propose that hook2 contributes to the establishment and maintenance of centrosomal structure and function. ..
- Wang P, Weng J, Lee S, Anderson R. The N terminus controls sterol binding while the C terminus regulates the scaffolding function of OSBP. J Biol Chem. 2008;283:8034-45 pubmed publisher..On the basis of this information we propose a new model for how OSBP is able to sense both membrane cholesterol and oxidized sterols and link this information to the ERK1/2 signaling pathway. ..
- Tuckerman J, Gonzalez G, Sousa E, Wan X, Saito J, Alam M, et al. An oxygen-sensing diguanylate cyclase and phosphodiesterase couple for c-di-GMP control. Biochemistry. 2009;48:9764-74 pubmed publisher..Finally, we find that DosP and DosC associate into a functional complex. We conclude that the dosCP operon encodes two oxygen sensors that cooperate in the controlled production and removal of c-di-GMP. ..
- Li J, Kato M, Chuang D. Pivotal role of the C-terminal DW-motif in mediating inhibition of pyruvate dehydrogenase kinase 2 by dichloroacetate. J Biol Chem. 2009;284:34458-67 pubmed publisher..These results implicate the DW-motif anchoring site as a drug target for the inhibition of aberrant PDK activity in cancer and diabetes. ..
- Schwartz J, Corey D. Practical considerations for analyzing antigene RNAs (agRNAs): RNA immunoprecipitation of argonaute protein. Methods Mol Biol. 2011;764:301-15 pubmed publisher..This technique can be used to detect interactions of argonaute proteins in the cell nucleus with their targets, lowly expressed noncoding RNA transcripts. ..