Experts and Doctors on endodeoxyribonucleases in Galveston, Texas, United States


Locale: Galveston, Texas, United States
Topic: endodeoxyribonucleases

Top Publications

  1. Habraken Y, Sung P, Prakash S, Prakash L. Transcription factor TFIIH and DNA endonuclease Rad2 constitute yeast nucleotide excision repair factor 3: implications for nucleotide excision repair and Cockayne syndrome. Proc Natl Acad Sci U S A. 1996;93:10718-22 pubmed
    ..Our findings are important for understanding the manner of assembly of the NER machinery and they have implications for Cockayne syndrome. ..
  2. Ribar B, Izumi T, Mitra S. The major role of human AP-endonuclease homolog Apn2 in repair of abasic sites in Schizosaccharomyces pombe. Nucleic Acids Res. 2004;32:115-26 pubmed
    ..We confirmed the presence of APE activity in recombinant Apn2 and in crude cell extracts. Thus S.pombe is distinct from S.cerevisiae, and is similar to mammalian cells in having Apn2 as the major APE. ..
  3. Piersen C, Prince M, Augustine M, Dodson M, Lloyd R. Purification and cloning of Micrococcus luteus ultraviolet endonuclease, an N-glycosylase/abasic lyase that proceeds via an imino enzyme-DNA intermediate. J Biol Chem. 1995;270:23475-84 pubmed
    ..These last findings confirm that the catalytic mechanism of M. luteus UV endonuclease, like those of other glycosylase/AP lyases, involves an imino intermediate...
  4. Zou Y, Walker R, Bassett H, Geacintov N, Van Houten B. Formation of DNA repair intermediates and incision by the ATP-dependent UvrB-UvrC endonuclease. J Biol Chem. 1997;272:4820-7 pubmed
    ..Incision of this Y structure occurs even without DNA damage. Thus the UvrBC complex is a structure-specific, ATP-dependent endonuclease. ..
  5. Zou Y, Van Houten B. Strand opening by the UvrA(2)B complex allows dynamic recognition of DNA damage. EMBO J. 1999;18:4889-901 pubmed
    ..These data support the hypothesis that the E.coli and human NER systems recognize and process DNA damage in a highly conserved manner. ..
  6. Torres Ramos C, Johnson R, Prakash L, Prakash S. Evidence for the involvement of nucleotide excision repair in the removal of abasic sites in yeast. Mol Cell Biol. 2000;20:3522-8 pubmed
    ..A deficiency in the repair of AP sites may contribute to the internal cancers and progressive neurodegeneration that occur in XP patients. ..
  7. Hazra T, Izumi T, Venkataraman R, Kow Y, Dizdaroglu M, Mitra S. Characterization of a novel 8-oxoguanine-DNA glycosylase activity in Escherichia coli and identification of the enzyme as endonuclease VIII. J Biol Chem. 2000;275:27762-7 pubmed
    ..The presence of OGG2 type enzyme in both E. coli and eukaryotes, which is at least as efficient in excising G* from a G*.A (or G) pair as from a G*.C pair, supports the possibility of G* repair in the nascent DNA strand. ..
  8. Lee S, Yu S, Prakash L, Prakash S. Requirement of yeast RAD2, a homolog of human XPG gene, for efficient RNA polymerase II transcription. implications for Cockayne syndrome. Cell. 2002;109:823-34 pubmed
    ..From these and other observations presented here, we suggest that transcriptional defects are the underlying cause of CS. ..
  9. Yu S, Lee S, Johnson R, Prakash L, Prakash S. The stalling of transcription at abasic sites is highly mutagenic. Mol Cell Biol. 2003;23:382-8 pubmed
    ..From the various observations presented here, we infer that the stalling of transcription at AP sites is highly mutagenic. ..

More Information


  1. Guzder S, Torres Ramos C, Johnson R, Haracska L, Prakash L, Prakash S. Requirement of yeast Rad1-Rad10 nuclease for the removal of 3'-blocked termini from DNA strand breaks induced by reactive oxygen species. Genes Dev. 2004;18:2283-91 pubmed
  2. Ma H, Wang J, Abdel Rahman S, Boor P, Khan M. Induction of base excision repair enzymes NTH1 and APE1 in rat spleen following aniline exposure. Toxicol Appl Pharmacol. 2013;267:276-83 pubmed publisher
    ..These findings thus identify a novel mechanism through which NTH1 and APE1 may regulate the repair of oxidative DNA damage in aniline-induced splenic toxicity. ..
  3. Ikeda S, Biswas T, Roy R, Izumi T, Boldogh I, Kurosky A, et al. Purification and characterization of human NTH1, a homolog of Escherichia coli endonuclease III. Direct identification of Lys-212 as the active nucleophilic residue. J Biol Chem. 1998;273:21585-93 pubmed
    ..Immunological studies showed that HeLa cells contain a single hNTH species of the predicted size, localized in both the nucleus and the cytoplasm. ..