Experts and Doctors on dicarboxylic acid transporters in Galveston, Texas, United States
Locale: Galveston, Texas, United States
Topic: dicarboxylic acid transporters
Zhang F, Pajor A. Topology of the Na(+)/dicarboxylate cotransporter: the N-terminus and hydrophilic loop 4 are located intracellularly. Biochim Biophys Acta. 2001;1511:80-9 pubmed
..The results of this study show that both the N-terminus and hydrophilic loop 4 of NaDC-1 are located intracellularly, which supports the current model of NaDC-1 structure. ..
Yao X, Pajor A. Arginine-349 and aspartate-373 of the Na(+)/dicarboxylate cotransporter are conformationally sensitive residues. Biochemistry. 2002;41:1083-90 pubmed
..We conclude that the accessibility of Arg-349 and Asp-373 is likely to change with the conformational states of the transport cycle. ..
Oshiro N, King S, Pajor A. Transmembrane helices 3 and 4 are involved in substrate recognition by the Na+/dicarboxylate cotransporter, NaDC1. Biochemistry. 2006;45:2302-10 pubmed
..We conclude that TM 3 and 4 contain amino acid residues that are important determinants of substrate specificity and catalytic efficiency in NaDC1. ..
Weerachayaphorn J, Pajor A. Identification of transport pathways for citric acid cycle intermediates in the human colon carcinoma cell line, Caco-2. Biochim Biophys Acta. 2008;1778:1051-9 pubmed publisher
..Since many of these pathways are not found in human intestine, this model may be best suited for studying Na+-dependent transport of succinate by NaDC1. ..
Pajor A, Sun N. Molecular cloning, chromosomal organization, and functional characterization of a sodium-dicarboxylate cotransporter from mouse kidney. Am J Physiol Renal Physiol. 2000;279:F482-90 pubmed
..However, its function more closely resembles the rabbit and human orthologs rather than the rat NaDC-1, with which it shares higher sequence similarity...
Pajor A, Randolph K. Conformationally sensitive residues in extracellular loop 5 of the Na+/dicarboxylate co-transporter. J Biol Chem. 2005;280:18728-35 pubmed
..Conformational changes in the protein affect the accessibility of the residues in extracellular loop 5 and provide further evidence of large-scale changes in the structure of NaDC-1 during the transport cycle. ..
Joshi A, Pajor A. Role of conserved prolines in the structure and function of the Na+/dicarboxylate cotransporter 1, NaDC1. Biochemistry. 2006;45:4231-9 pubmed
..The prolines in TM 10, at positions 523 and 524, may not be directly involved in the transporter function but may be necessary for maintaining structure. ..
Oshiro N, Pajor A. Ala-504 is a determinant of substrate binding affinity in the mouse Na(+)/dicarboxylate cotransporter. Biochim Biophys Acta. 2006;1758:781-8 pubmed
..We conclude that multiple amino acids from TM 8, 9 and 10 contribute to the transport of adipate in NaDC1. Furthermore, Ala-504 in TM 10 is an important determinant of K(0.5) for both adipate and succinate. ..
Weerachayaphorn J, Pajor A. Threonine-509 is a determinant of apparent affinity for both substrate and cations in the human Na+/dicarboxylate cotransporter. Biochemistry. 2008;47:1087-93 pubmed
..Furthermore, the results suggest that the cation and substrate binding sites are located in close proximity to one another in NaDC1. ..