Genomes and Genes
Experts and Doctors on caenorhabditis elegans in Dallas, Texas, United States
Locale: Dallas, Texas, United States
Topic: caenorhabditis elegans
- You Y, Kim J, Cobb M, Avery L. Starvation activates MAP kinase through the muscarinic acetylcholine pathway in Caenorhabditis elegans pharynx. Cell Metab. 2006;3:237-45 pubmed..We suggest that, during starvation, the muscarinic pathway to MAPK is activated to change the pharyngeal muscle physiology to enhance ingestion of food when food becomes available. ..
- Karakuzu O, Wang D, Cameron S. MIG-32 and SPAT-3A are PRC1 homologs that control neuronal migration in Caenorhabditis elegans. Development. 2009;136:943-53 pubmed publisher..However, unlike the mes mutants, mig-32 and spat-3 mutants are fertile, suggesting that PRC1 function is not absolutely required in the germline for essential functions of PRC2. ..
- Steger K, Shtonda B, Thacker C, Snutch T, Avery L. The C. elegans T-type calcium channel CCA-1 boosts neuromuscular transmission. J Exp Biol. 2005;208:2191-203 pubmed..We also show that the pharyngeal muscle employs alternative strategies for initiating action potentials in certain cases of compromised MC motor neuron function. ..
- Liu Q, Rand T, Kalidas S, Du F, Kim H, Smith D, et al. R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway. Science. 2003;301:1921-5 pubmed..These results indicate that R2D2 bridges the initiation and effector steps of the Drosophila RNAi pathway by facilitating siRNA passage from Dicer to RISC. ..
- Kramer H. The ups and downs of life in an epithelium. J Cell Biol. 2000;151:F15-8 pubmed
- Peng S, Crider B, Tsai S, Xie X, Stone D. Identification of a 14-kDa subunit associated with the catalytic sector of clathrin-coated vesicle H+-ATPase. J Biol Chem. 1996;271:3324-7 pubmed..In addition, direct sequencing of this 14-kDa component of the coated vacuolar proton pump confirmed its identity as a subunit F homologue. ..
- Lee R, Sawin E, Chalfie M, Horvitz H, Avery L. EAT-4, a homolog of a mammalian sodium-dependent inorganic phosphate cotransporter, is necessary for glutamatergic neurotransmission in caenorhabditis elegans. J Neurosci. 1999;19:159-67 pubmed..Our data suggest that phosphate ions imported into glutamatergic neurons through transporters such as EAT-4 and BNPI are required specifically for glutamatergic neurotransmission. ..
- Kosarek J, Woodruff R, Rivera Begeman A, Guo C, D Souza S, Koonin E, et al. Comparative analysis of in vivo interactions between Rev1 protein and other Y-family DNA polymerases in animals and yeasts. DNA Repair (Amst). 2008;7:439-51 pubmed publisher..The results of this study suggest that special consideration should be exercised when making mechanistic extrapolations regarding translesion DNA synthesis from one eukaryotic system to another. ..
- Shtonda B, Avery L. CCA-1, EGL-19 and EXP-2 currents shape action potentials in the Caenorhabditis elegans pharynx. J Exp Biol. 2005;208:2177-90 pubmed..Our results suggest that EXP-2 is a potassium channel with unusual properties that uses a hyperpolarization threshold to activate a regenerative hyperpolarizing current. ..
- zkan E, Chia P, Wang R, Goriatcheva N, Borek D, Otwinowski Z, et al. Extracellular architecture of the SYG-1/SYG-2 adhesion complex instructs synaptogenesis. Cell. 2014;156:482-94 pubmed publisher..These results suggest that SYG extracellular complexes do not simply act as "molecular velcro" and that their distinct structural features are important in instructing synaptogenesis. PAPERFLICK:..
- Park S, Bin N, Yu B, Wong R, Sitarska E, Sugita K, et al. UNC-18 and Tomosyn Antagonistically Control Synaptic Vesicle Priming Downstream of UNC-13 in Caenorhabditis elegans. J Neurosci. 2017;37:8797-8815 pubmed publisher..Thus, our study provides novel mechanistic insights into how Munc18/UNC-18 primes synaptic vesicle release and how this protein interacts functionally with Munc13/UNC-13 and Tomosyn/TOM-1. ..
- Jia K, Levine B. Autophagy is required for dietary restriction-mediated life span extension in C. elegans. Autophagy. 2007;3:597-9 pubmed..elegans life span. Since autophagy and longevity control are highly conserved from C. elegans to mammals, a similar role for autophagy in dietary restriction-mediated life span extension may also exist in mammals. ..
- Zubovych I, Straud S, Roth M. Mitochondrial dysfunction confers resistance to multiple drugs in Caenorhabditis elegans. Mol Biol Cell. 2010;21:956-69 pubmed publisher..elegans. Using genetics, we show that this drug resistance requires pkc-1, the C. elegans ortholog of human PKCepsilon. ..
- Chia P, Chen B, Li P, Rosen M, Shen K. Local F-actin network links synapse formation and axon branching. Cell. 2014;156:208-20 pubmed publisher..Together, these data suggest that synaptic adhesion molecules, which serve as a necessary component for both synaptogenesis and axonal branch formation, directly regulate subcellular actin cytoskeletal organization. ..
- Straud S, Lee I, Song B, Avery L, You Y. The jaw of the worm: GTPase-activating protein EAT-17 regulates grinder formation in Caenorhabditis elegans. Genetics. 2013;195:115-25 pubmed publisher..2. Based on the conserved function of Rab6 in vesicular transport, we propose that EAT-17 regulates the turnover rate of RAB-6.2 activity in cargo trafficking for grinder formation. ..
- Davis M, Somerville D, Lee R, Lockery S, Avery L, Fambrough D. Mutations in the Caenorhabditis elegans Na,K-ATPase alpha-subunit gene, eat-6, disrupt excitable cell function. J Neurosci. 1995;15:8408-18 pubmed..To explain these abnormalities, we propose that a reduction of Na,K-ATPase activity in eat-6 mutants leads to a reduction of the ion concentration gradients that power membrane potential changes. ..
- Peters J, McKay R, McKay J, Graff J. Casein kinase I transduces Wnt signals. Nature. 1999;401:345-50 pubmed..In addition, CKI bound to and increased the phosphorylation of dishevelled, a known component of the Wnt pathway. These data indicate that CKI may be a conserved component of the Wnt pathway. ..
- Jia K, Hart A, Levine B. Autophagy genes protect against disease caused by polyglutamine expansion proteins in Caenorhabditis elegans. Autophagy. 2007;3:21-5 pubmed..These data provide in vivo genetic evidence that autophagy genes suppress the accumulation of polyQ aggregates and protect cells from disease caused by polyQ toxicity. ..
- Dent J, Smith M, Vassilatis D, Avery L. The genetics of ivermectin resistance in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2000;97:2674-9 pubmed..Our results suggest that the evolution of drug resistance can be slowed by targeting antibiotic drugs to several members of a multigene family. ..
- Shin O, Han W, Wang Y, Sudhof T. Evolutionarily conserved multiple C2 domain proteins with two transmembrane regions (MCTPs) and unusual Ca2+ binding properties. J Biol Chem. 2005;280:1641-51 pubmed
- Berman K, Hutchison M, Avery L, Cobb M. kin-18, a C. elegans protein kinase involved in feeding. Gene. 2001;279:137-47 pubmed..We have also identified a C. elegans gene that encodes a protein kinase similar to mammalian MAPK/ERK Kinase (MEK) 4 whose promoter is active in the pharynx. It is phosphorylated by TAO1 in vitro and physically interacts with TAO1. ..
- Song B, Avery L. Serotonin activates overall feeding by activating two separate neural pathways in Caenorhabditis elegans. J Neurosci. 2012;32:1920-31 pubmed publisher..2004), we propose a model that explains how the two feeding motions are separately regulated yet coupled. The feeding organ may have evolved this way to support efficient feeding. ..
- Keane J, Avery L. Mechanosensory inputs influence Caenorhabditis elegans pharyngeal activity via ivermectin sensitivity genes. Genetics. 2003;164:153-62 pubmed
- Li C, Tucker P. DNA-binding properties and secondary structural model of the hepatocyte nuclear factor 3/fork head domain. Proc Natl Acad Sci U S A. 1993;90:11583-7 pubmed..An aromatic kink and a third amphipathic helix comprise the center of the domain. At the C terminus, two variable-length loops flank a putative 7-amino acid helix followed by a short basic region. ..
- Han S, Bahmanyar S, Zhang P, Grishin N, Oegema K, Crooke R, et al. Nuclear envelope phosphatase 1-regulatory subunit 1 (formerly TMEM188) is the metazoan Spo7p ortholog and functions in the lipin activation pathway. J Biol Chem. 2012;287:3123-37 pubmed publisher..The nuclear fraction of lipin-1b is increased when CTDNEP1 and NEP1-R1 are co-expressed. Therefore, NEP1-R1 is functionally conserved from yeast to humans and functions in the lipin activation pathway. ..
- Fernandez Chacon R, Sudhof T. Novel SCAMPs lacking NPF repeats: ubiquitous and synaptic vesicle-specific forms implicate SCAMPs in multiple membrane-trafficking functions. J Neurosci. 2000;20:7941-50 pubmed
- Wilkie T. G proteins, chemosensory perception, and the C. elegans genome project: An attractive story. Bioessays. 1999;21:713-7 pubmed..The orthologous genes are widely expressed, whereas 14 of the divergent Galpha genes are almost exclusively expressed in sensory neurons where they may regulate perception and chemotaxis. ..
- McKay R, McKay J, Avery L, Graff J. C elegans: a model for exploring the genetics of fat storage. Dev Cell. 2003;4:131-42 pubmed..A third encodes lpd-3, whose homolog is also required for fat storage in a mammalian model. These data suggest that C. elegans is a genetically tractable model to study the mechanisms that underlie the biology of fat-storing tissues. ..
- Kang C, You Y, Avery L. Dual roles of autophagy in the survival of Caenorhabditis elegans during starvation. Genes Dev. 2007;21:2161-71 pubmed..Taken together, our results demonstrate that autophagy can have either prosurvival or prodeath functions in an organism, depending on its level of activation. ..
- Rogers E, Bishop J, Waddle J, Schumacher J, Lin R. The aurora kinase AIR-2 functions in the release of chromosome cohesion in Caenorhabditis elegans meiosis. J Cell Biol. 2002;157:219-29 pubmed..We propose that AIR-2 promotes the release of chromosome cohesion via phosphorylation of REC-8 at specific chromosomal locations and that CeGLC-7alpha/beta, directly or indirectly, antagonize AIR-2 activity. ..
- Robertson S, Shetty P, Lin R. Identification of lineage-specific zygotic transcripts in early Caenorhabditis elegans embryos. Dev Biol. 2004;276:493-507 pubmed..These results demonstrate the successful combination of single-staged embryo cDNAs, genetic mutants, and whole transcriptome microarray analysis to identify stage- and lineage-specific transcripts in early C. elegans embryos. ..
- Hata Y, Slaughter C, Sudhof T. Synaptic vesicle fusion complex contains unc-18 homologue bound to syntaxin. Nature. 1993;366:347-51 pubmed..Our data suggest that Munc-18 is a previously unidentified essential component of the synaptic vesicle fusion protein complex. ..
- Avery L. Caenorhabditis elegans behavioral genetics: where are the knobs?. BMC Biol. 2010;8:69 pubmed publisher..I suggest a set of criteria by which some genes important in the evolution of behavior might be recognized, and identify neuropeptide signaling pathways as candidates. ..
- McKay J, Raizen D, Gottschalk A, Schafer W, Avery L. eat-2 and eat-18 are required for nicotinic neurotransmission in the Caenorhabditis elegans pharynx. Genetics. 2004;166:161-9 pubmed..In eat-18 mutants, the gross localization of EAT-2 at the MC synapse is normal, suggesting that it is not required for trafficking. These data indicate that eat-18 could be a novel component of the pharyngeal nicotinic receptor. ..
- Steger K, Avery L. The GAR-3 muscarinic receptor cooperates with calcium signals to regulate muscle contraction in the Caenorhabditis elegans pharynx. Genetics. 2004;167:633-43 pubmed..Because the effects of GAR-3 signaling on membrane depolarization and muscle contraction can be separated, we conclude that GAR-3 regulates multiple calcium-dependent processes in the C. elegans pharyngeal muscle. ..
- Yang X, Huang S, Lo M, Mizumoto K, Sawa H, Xu W, et al. Distinct and mutually inhibitory binding by two divergent ?-catenins coordinates TCF levels and activity in C. elegans. Development. 2011;138:4255-65 pubmed publisher..These studies could provide novel insights into cancers arising from aberrant Wnt activation. ..
- Das R, Melo J, Thondamal M, Morton E, Cornwell A, Crick B, et al. The homeodomain-interacting protein kinase HPK-1 preserves protein homeostasis and longevity through master regulatory control of the HSF-1 chaperone network and TORC1-restricted autophagy in Caenorhabditis elegans. PLoS Genet. 2017;13:e1007038 pubmed publisher..HPK-1 therefore provides a promising intervention point for pharmacological agents targeting the protein homeostasis system as a means of preserving robust longevity. ..
- Guven Ozkan T, Nishi Y, Robertson S, Lin R. Global transcriptional repression in C. elegans germline precursors by regulated sequestration of TAF-4. Cell. 2008;135:149-60 pubmed publisher..We propose that phosphorylation by MBK-2 serves as a developmental switch, converting OMA-1/2 from oocyte to embryo regulators. ..
- Avery L. The genetics of feeding in Caenorhabditis elegans. Genetics. 1993;133:897-917 pubmed..eat-6 and eat-12 mutants failed to relax their pharyngeal muscles properly. These pharyngeal motion defects are most easily explained as resulting from abnormal electrical excitability of the pharyngeal muscle membrane. ..
- Janz R, Hofmann K, Sudhof T. SVOP, an evolutionarily conserved synaptic vesicle protein, suggests novel transport functions of synaptic vesicles. J Neurosci. 1998;18:9269-81 pubmed
- Huang S, Shetty P, Robertson S, Lin R. Binary cell fate specification during C. elegans embryogenesis driven by reiterated reciprocal asymmetry of TCF POP-1 and its coactivator beta-catenin SYS-1. Development. 2007;134:2685-95 pubmed..We propose that two genetic pathways, one increasing SYS-1 and the other decreasing POP-1 levels, robustly elevate the SYS-1-to-POP-1 ratio in the posterior cell, thereby driving A-P differential cell fates. ..
- Nishi Y, Lin R. DYRK2 and GSK-3 phosphorylate and promote the timely degradation of OMA-1, a key regulator of the oocyte-to-embryo transition in C. elegans. Dev Biol. 2005;288:139-49 pubmed..elegans. ..
- Hua X, Nohturfft A, Goldstein J, Brown M. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein. Cell. 1996;87:415-26 pubmed..SCAP appears to be a central regulator of cholesterol metabolism in animal cells...
- Oldenbroek M, Robertson S, Guven Ozkan T, Spike C, Greenstein D, Lin R. Regulation of maternal Wnt mRNA translation in C. elegans embryos. Development. 2013;140:4614-23 pubmed publisher..We propose that the 3'UTR of maternal mRNAs contains a combinatorial code that determines the topography of associated RBPs, integrating positive and negative translational inputs. ..
- MacQueen A, Baggett J, Perumov N, Bauer R, Januszewski T, Schriefer L, et al. ACT-5 is an essential Caenorhabditis elegans actin required for intestinal microvilli formation. Mol Biol Cell. 2005;16:3247-59 pubmed
- Lin R. A gain-of-function mutation in oma-1, a C. elegans gene required for oocyte maturation, results in delayed degradation of maternal proteins and embryonic lethality. Dev Biol. 2003;258:226-39 pubmed..These observations suggest that oma-1, in addition to its role in oocyte maturation, contributes to early embryonic development by regulating the temporal degradation of maternal proteins in early C. elegans embryos. ..
- Igarashi P. Overview: nonmammalian organisms for studies of kidney development and disease. J Am Soc Nephrol. 2005;16:296-8 pubmed
- Nishi Y, Rogers E, Robertson S, Lin R. Polo kinases regulate C. elegans embryonic polarity via binding to DYRK2-primed MEX-5 and MEX-6. Development. 2008;135:687-97 pubmed publisher..Our results provide a mechanism by which MEX-5 and MEX-6 function is temporally regulated during the crucial oocyte-to-embryo transition. ..
- Guo Y, Chen S, Shetty P, Zheng G, Lin R, Li W. Imaging dynamic cell-cell junctional coupling in vivo using Trojan-LAMP. Nat Methods. 2008;5:835-41 pubmed publisher..As dextran-CANPE-HCC is chemically and metabolically stable, Labeled worms showed very bright signal upon photoactivation after hatching, which allowed us to examine cell coupling in living worms noninvasively. ..
- Liu H, Strauss T, Potts M, Cameron S. Direct regulation of egl-1 and of programmed cell death by the Hox protein MAB-5 and by CEH-20, a C. elegans homolog of Pbx1. Development. 2006;133:641-50 pubmed..Direct regulation of programmed cell death may be an evolutionarily ancient and conserved function of Hox genes. ..
- Berman K, McKay J, Avery L, Cobb M. Isolation and characterization of pmk-(1-3): three p38 homologs in Caenorhabditis elegans. Mol Cell Biol Res Commun. 2001;4:337-44 pubmed..PMK-1 and PMK-2 phosphorylated activating transcription factor-2 (ATF-2), indicating an activity similar to mammalian p38. When transfected into mammalian cells, these kinases, like p38, are stimulated by osmotic stresses. ..
- McKay R, McKay J, Suh J, Avery L, Graff J. Tripeptidyl peptidase II promotes fat formation in a conserved fashion. EMBO Rep. 2007;8:1183-9 pubmed..These findings indicate that TPPII has central and peripheral roles in regulating metabolism and that TPPII actions in fat-storing tissues might be an ancient function carried out in a protease-independent manner. ..
- Yu S, Avery L, Baude E, Garbers D. Guanylyl cyclase expression in specific sensory neurons: a new family of chemosensory receptors. Proc Natl Acad Sci U S A. 1997;94:3384-7 pubmed..Thus, the guanylyl cyclases represent an unexpectedly large and new family of sensory neuron receptors that may complement the 7-transmembrane family of odorant/pheromone receptors. ..
- Eberhart C, Wasserman S. The pelota locus encodes a protein required for meiotic cell division: an analysis of G2/M arrest in Drosophila spermatogenesis. Development. 1995;121:3477-86 pubmed..pelota is also required for patterning in the eye and mitotic divisions in the ovary. We have cloned the pelota locus and show it encodes a 44 x 10(3) M(r) protein with yeast, plant, worm and human homologs. ..
- Lo M, Gay F, Odom R, Shi Y, Lin R. Phosphorylation by the beta-catenin/MAPK complex promotes 14-3-3-mediated nuclear export of TCF/POP-1 in signal-responsive cells in C. elegans. Cell. 2004;117:95-106 pubmed..Our results suggest a model whereby Wnt/MAPK signaling downregulates POP-1 levels in responsive cells, in part by increasing nuclear LIT-1 levels, thereby increasing POP-1 phosphorylation and PAR-5-mediated nuclear export. ..
- Motola D, Cummins C, Rottiers V, Sharma K, Li T, Li Y, et al. Identification of ligands for DAF-12 that govern dauer formation and reproduction in C. elegans. Cell. 2006;124:1209-23 pubmed
- Jia K, Thomas C, Akbar M, Sun Q, Adams Huet B, Gilpin C, et al. Autophagy genes protect against Salmonella typhimurium infection and mediate insulin signaling-regulated pathogen resistance. Proc Natl Acad Sci U S A. 2009;106:14564-9 pubmed publisher..Thus, autophagy genes play an essential role in host defense in vivo against an intracellular bacterial pathogen and mediate pathogen resistance in long-lived mutant nematodes. ..
- You Y, Kim J, Raizen D, Avery L. Insulin, cGMP, and TGF-beta signals regulate food intake and quiescence in C. elegans: a model for satiety. Cell Metab. 2008;7:249-57 pubmed publisher..The EGL-4 cGMP-dependent protein kinase functions downstream of insulin and TGF-beta in sensory neurons including ASI to control quiescence in response to food intake. ..
- Shetty P, Lo M, Robertson S, Lin R. C. elegans TCF protein, POP-1, converts from repressor to activator as a result of Wnt-induced lowering of nuclear levels. Dev Biol. 2005;285:584-92 pubmed..We propose that the balance between TCF/LEF and coactivator(s), achieved by elevating coactivator levels (the canonical pathway) and/or reducing TCF/LEF levels (worm endoderm), determines Wnt signal strength. ..