Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in Nashville, Tennessee, United States
Locale: Nashville, Tennessee, United States
Topic: saccharomyces cerevisiae
Publications129 found, 100 shown here
- Bromberg K, Osheroff N. DNA cleavage and religation by human topoisomerase II alpha at high temperature. Biochemistry. 2001;40:8410-8 pubmed..Finally, similar high-temperature alterations were observed for yeast topoisomerase II and human topoisomerase IIbeta, suggesting that parallel heat-induced transitions may be widespread among type II topoisomerases. ..
- Chatterjee D, Mukherjee S, Das S. Development and characterization of cholinephosphotransferase antibody. Biochem Biophys Res Commun. 2001;285:965-8 pubmed..Further analysis revealed that the antibody inhibited CPT activity in both subcellular fractions in a dose dependent manner, thus confirming the specificity of the antibody against both subcellular CPT. ..
- Suntharalingam M, Alcázar Román A, Wente S. Nuclear export of the yeast mRNA-binding protein Nab2 is linked to a direct interaction with Gfd1 and to Gle1 function. J Biol Chem. 2004;279:35384-91 pubmed..As Nab2 export was blocked in a gle1 mutant at the restrictive temperature, we propose a model wherein Gfd1 serves as a bridging factor between Gle1 and Nab2-bound mRNA during export. ..
- Xiao J, Kim L, Graham T. Dissection of Swa2p/auxilin domain requirements for cochaperoning Hsp70 clathrin-uncoating activity in vivo. Mol Biol Cell. 2006;17:3281-90 pubmed..These data suggest that the TPR and J-domains collaborate in a bipartite interaction with Hsp70 to regulate its activity in clathrin disassembly. ..
- Clifford D, Chen C, Roberts R, Feoktistova A, Wolfe B, Chen J, et al. The role of Cdc14 phosphatases in the control of cell division. Biochem Soc Trans. 2008;36:436-8 pubmed publisher..Here, we review the elucidated roles of Cdc14 phosphatases in cytokinesis and the current outstanding questions regarding their function in this process. ..
- Mullins C, Meyer H, Hartmann E, Green N, Fang H. Structurally related Spc1p and Spc2p of yeast signal peptidase complex are functionally distinct. J Biol Chem. 1996;271:29094-9 pubmed
- Chen C, Ingram M, Rosal P, Graham T. Role for Drs2p, a P-type ATPase and potential aminophospholipid translocase, in yeast late Golgi function. J Cell Biol. 1999;147:1223-36 pubmed..These observations indicate a novel role for a P-type ATPase in late Golgi function and suggest a possible link between membrane asymmetry and clathrin function at the Golgi complex. ..
- Chen X, VanValkenburgh C, Liang H, Fang H, Green N. Signal peptidase and oligosaccharyltransferase interact in a sequential and dependent manner within the endoplasmic reticulum. J Biol Chem. 2001;276:2411-6 pubmed
- Miao M, Ryan K, Wente S. The integral membrane protein Pom34p functionally links nucleoporin subcomplexes. Genetics. 2006;172:1441-57 pubmed..We speculate that multiple integral membrane proteins, either within the nuclear pore domain or in the nuclear envelope, execute coordinated roles in NPC structure and function. ..
- Kanter D, Bruck I, Kaplan D. Mcm subunits can assemble into two different active unwinding complexes. J Biol Chem. 2008;283:31172-82 pubmed publisher..We conclude that the Mcm subunits are surprisingly flexible and dynamic in their ability to interact with one another to form active unwinding complexes. ..
- Muthusamy B, Natarajan P, Zhou X, Graham T. Linking phospholipid flippases to vesicle-mediated protein transport. Biochim Biophys Acta. 2009;1791:612-9 pubmed publisher..Here, we discuss the physiological requirements for yeast P4-ATPases in phospholipid translocase activity, transport vesicle budding and ergosterol metabolism, with an emphasis on Drs2p and its noncatalytic subunit, Cdc50p. ..
- Carmody S, Tran E, Apponi L, Corbett A, Wente S. The mitogen-activated protein kinase Slt2 regulates nuclear retention of non-heat shock mRNAs during heat shock-induced stress. Mol Cell Biol. 2010;30:5168-79 pubmed publisher..These results reveal a novel mechanism by which the MAP kinase Slt2 and Mlp1 control mRNA export factors during heat shock stress. ..
- Ferguson J, Chao W, Lee E, Friedman K. The anaphase promoting complex contributes to the degradation of the S. cerevisiae telomerase recruitment subunit Est1p. PLoS ONE. 2013;8:e55055 pubmed publisher..Together, these studies shed light on the regulation of yeast telomerase assembly and demonstrate a new connection between telomere maintenance and cell cycle regulation pathways. ..
- Klebanow E, Poon D, Zhou S, Weil P. Cloning and characterization of an essential Saccharomyces cerevisiae gene, TAF40, which encodes yTAFII40, an RNA polymerase II-specific TATA-binding protein-associated factor. J Biol Chem. 1997;272:9436-42 pubmed..By these criteria yTAF40 appears to encode a bona fide RNA polymerase II-specific TAF, and thus the protein that it encodes has been termed yTAFII40. ..
- Flick J, Thorner J. An essential function of a phosphoinositide-specific phospholipase C is relieved by inhibition of a cyclin-dependent protein kinase in the yeast Saccharomyces cerevisiae. Genetics. 1998;148:33-47 pubmed..Unlike single mutants, plc1delta pho81delta and plc1delta spl2delta double mutants were unable to grow on synthetic complete medium, but were able to grow on rich medium. ..
- Ohi M, Vander Kooi C, Rosenberg J, Ren L, Hirsch J, Chazin W, et al. Structural and functional analysis of essential pre-mRNA splicing factor Prp19p. Mol Cell Biol. 2005;25:451-60 pubmed..These structural and functional data provide a basis for understanding the role of Prp19p as a key architectural component of the NTC. ..
- Liu K, Hua Z, Nepute J, Graham T. Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway. Mol Biol Cell. 2007;18:487-500 pubmed..These results demonstrate an essential role for endocytosis in retrieving proteins back to the Golgi, and they define critical cargos of the NPFXD/Sla1p system...
- Salichos L, Rokas A. Evaluating ortholog prediction algorithms in a yeast model clade. PLoS ONE. 2011;6:e18755 pubmed publisher..g., molecular phylogenetics), but that all algorithms fail to accurately predict orthologs when paralogy is rampant. ..
- Geng F, Tansey W. Similar temporal and spatial recruitment of native 19S and 20S proteasome subunits to transcriptionally active chromatin. Proc Natl Acad Sci U S A. 2012;109:6060-5 pubmed publisher..We also find that proteasome subunits are enriched at telomeres and at genes transcribed by RNA polymerase III. Our data suggest that the transcriptionally relevant form of the proteasome is the canonical 26S complex. ..
- Zhang Y, Xie Q, Robertson J, Johnson C. pHlash: a new genetically encoded and ratiometric luminescence sensor of intracellular pH. PLoS ONE. 2012;7:e43072 pubmed publisher..This is the first BRET-based sensor of H(+) ions, and it should allow the approximation of pH in cytosolic and organellar compartments in applications where current pH probes are inadequate. ..
- Elsea S, Osheroff N, Nitiss J. Cytotoxicity of quinolones toward eukaryotic cells. Identification of topoisomerase II as the primary cellular target for the quinolone CP-115,953 in yeast. J Biol Chem. 1992;267:13150-3 pubmed..These results strongly suggest that topoisomerase II is the primary physiological target responsible for quinolone cytotoxicity and that CP-115,953 kills cells by converting the type II enzyme into a cellular poison. ..
- Chinkers M, Garbers D, Chang M, Lowe D, Chin H, Goeddel D, et al. A membrane form of guanylate cyclase is an atrial natriuretic peptide receptor. Nature. 1989;338:78-83 pubmed..The ANP receptor/guanylate cyclase represents a new class of mammalian cell-surface receptors which contain an extracellular ligand-binding domain and an intracellular guanylate cyclase catalytic domain. ..
- Klebanow E, Poon D, Zhou S, Weil P. Isolation and characterization of TAF25, an essential yeast gene that encodes an RNA polymerase II-specific TATA-binding protein-associated factor. J Biol Chem. 1996;271:13706-15 pubmed..Hence the protein encoded by TAF25 has been termed yTAFII25. ..
- Mordes D, Glick G, Zhao R, Cortez D. TopBP1 activates ATR through ATRIP and a PIKK regulatory domain. Genes Dev. 2008;22:1478-89 pubmed publisher..Therefore, divergent amino acid sequences within the PRD and a unique protein partner allow each of these PIK kinases to respond to distinct cellular events. ..
- Farley A, Powell D, Weaver C, Jennings J, Link A. Assessing the components of the eIF3 complex and their phosphorylation status. J Proteome Res. 2011;10:1481-94 pubmed publisher..Replacement of these three Nip1 serines with alanines causes a slow growth phenotype. ..
- Parsons M, Weil P. Purification and characterization of Saccharomyces cerevisiae transcription factor TFIIIC. Polypeptide composition defined with polyclonal antibodies. J Biol Chem. 1990;265:5095-103 pubmed..These results suggested that these three polypeptides were tightly associated with one another in solution. ..
- Felts S, Weil P, Chalkley R. Transcription factor requirements for in vitro formation of transcriptionally competent 5S rRNA gene chromatin. Mol Cell Biol. 1990;10:2390-401 pubmed..The patterns of protein-DNA interactions formed on these genes are consistent with these DNAs being assembled into extensive nucleosomal arrays. ..
- Poon D, Bai Y, Campbell A, Bjorklund S, Kim Y, Zhou S, et al. Identification and characterization of a TFIID-like multiprotein complex from Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1995;92:8224-8 pubmed..In addition, by constructing null alleles of the cloned TAF-encoding genes, we show that normal function of the TAF-encoding genes is essential for yeast cell viability. ..
- Graham T, Seeger M, Payne G, MacKay V, Emr S. Clathrin-dependent localization of alpha 1,3 mannosyltransferase to the Golgi complex of Saccharomyces cerevisiae. J Cell Biol. 1994;127:667-78 pubmed..These results indicate that clathrin plays a broader role in the localization of Golgi proteins than anticipated. ..
- Fang H, Panzner S, Mullins C, Hartmann E, Green N. The homologue of mammalian SPC12 is important for efficient signal peptidase activity in Saccharomyces cerevisiae. J Biol Chem. 1996;271:16460-5 pubmed..Through both genetic and biochemical criteria, Spc1p appears, however, to be important for efficient signal peptidase activity. ..
- Chen C, Graham T. An arf1Delta synthetic lethal screen identifies a new clathrin heavy chain conditional allele that perturbs vacuolar protein transport in Saccharomyces cerevisiae. Genetics. 1998;150:577-89 pubmed
- Coolidge C, Patton J. A new double-stranded RNA-binding protein that interacts with PKR. Nucleic Acids Res. 2000;28:1407-17 pubmed..We hypothesize that competing heterodimers between p74-K296R PKR and eIF2alpha-K296R PKR may control cell growth such that stabilization of the p74-K296R PKR heterodimer induces abnormal morphology and cell death. ..
- Hua Z, Graham T. Requirement for neo1p in retrograde transport from the Golgi complex to the endoplasmic reticulum. Mol Biol Cell. 2003;14:4971-83 pubmed..We propose that loss of lipid asymmetry in the cis Golgi perturbs retrograde protein transport to the ER. ..
- Peng R, Hawkins I, Link A, Patton J. The splicing factor PSF is part of a large complex that assembles in the absence of pre-mRNA and contains all five snRNPs. RNA Biol. 2006;3:69-76 pubmed..Sedimentation experiments and identification of individual components by mass spectrometry revealed association with multiple nuclear factors, most of which overlap with spliceosome components. ..
- Seale K, Janetopoulos C, Wikswo J. Micro-mirrors for nanoscale three-dimensional microscopy. ACS Nano. 2009;3:493-7 pubmed publisher..As inexpensive tools for 3D visualization, mirrored pyramidal wells may prove to be invaluable aids in nanotechnology and engineering of nanomaterials. ..
- Wente S, Rout M. The nuclear pore complex and nuclear transport. Cold Spring Harb Perspect Biol. 2010;2:a000562 pubmed publisher..All these requirements demonstrate the complex design of the NPC and the integral role it plays in key cellular processes...
- Bolger T, Wente S. Gle1 is a multifunctional DEAD-box protein regulator that modulates Ded1 in translation initiation. J Biol Chem. 2011;286:39750-9 pubmed publisher..Together, the differential regulation of two distinct DEAD-box proteins by a common factor (Gle1) establishes a new paradigm for controlling gene expression and coupling translation with mRNA export. ..
- Poon D, Campbell A, Bai Y, Weil P. Yeast Taf170 is encoded by MOT1 and exists in a TATA box-binding protein (TBP)-TBP-associated factor complex distinct from transcription factor IID. J Biol Chem. 1994;269:23135-40 pubmed..The significance of this unique TBP-Taf170 complex regarding transcriptional regulation is discussed. ..
- Stein E, Cerretti D, Daniel T. Ligand activation of ELK receptor tyrosine kinase promotes its association with Grb10 and Grb2 in vascular endothelial cells. J Biol Chem. 1996;271:23588-93 pubmed..These findings define ligand-activated interaction between ELK and the SH2 domains of Grb2 and the newly identified Grb10 protein that shares homology with a Caenorhabditis elegans gene product implicated in neural cell migration. ..
- Berry L, Gould K. Fission yeast dim1(+) encodes a functionally conserved polypeptide essential for mitosis. J Cell Biol. 1997;137:1337-54 pubmed..Based on TBZ sensitivity and failed chromosome segregation in dim1-35, we further speculate that Dim1p may play a role in mitotic spindle formation and/or function. ..
- Patterson G, Schroeder S, Bai Y, Weil A, Piston D. Quantitative imaging of TATA-binding protein in living yeast cells. Yeast. 1998;14:813-25 pubmed..7) between mother and daughter cells. Based on this and data from a mutant which underexpresses GFP x TBP, we suggest that intracellular levels of TBP are near rate-limiting for growth and viability. ..
- Banik U, Beechem J, Klebanow E, Schroeder S, Weil P. Fluorescence-based analyses of the effects of full-length recombinant TAF130p on the interaction of TATA box-binding protein with TATA box DNA. J Biol Chem. 2001;276:49100-9 pubmed..Interestingly, we have found that full-length TAF130p is capable of binding multiple molecules of TBP with nanomolar binding affinity. The biological implications of these findings are discussed. ..
- Sanders S, Garbett K, Weil P. Molecular characterization of Saccharomyces cerevisiae TFIID. Mol Cell Biol. 2002;22:6000-13 pubmed..The results of direct biochemical exchange experiments confirmed this hypothesis. Together, our results represent a concise molecular characterization of the general transcription factor TFIID from S. cerevisiae. ..
- Strawn L, Shen T, Shulga N, Goldfarb D, Wente S. Minimal nuclear pore complexes define FG repeat domains essential for transport. Nat Cell Biol. 2004;6:197-206 pubmed..Significantly, symmetric deletions caused mild reductions in Kap95-Kap60-mediated import rates, but virtually abolished Kap104 import. These results suggest the existence of multiple translocation pathways. ..
- Lee Y, Naider F, Becker J. Interacting residues in an activated state of a G protein-coupled receptor. J Biol Chem. 2006;281:2263-72 pubmed..The information gained from this study should contribute to an understanding of the conformational differences between resting and active states in GPCRs. ..
- Osterhage J, Talley J, Friedman K. Proteasome-dependent degradation of Est1p regulates the cell cycle-restricted assembly of telomerase in Saccharomyces cerevisiae. Nat Struct Mol Biol. 2006;13:720-8 pubmed..We conclude that telomerase assembly during G1 phase is regulated by Est1p stability, but assembly is insufficient to activate telomerase at telomeres. ..
- Ryan K, Zhou Y, Wente S. The karyopherin Kap95 regulates nuclear pore complex assembly into intact nuclear envelopes in vivo. Mol Biol Cell. 2007;18:886-98 pubmed..We conclude that Kap95 serves as a key regulator of NPC assembly into intact NEs. Furthermore, both Kap95 and Ran may provide spatial cues necessary for targeting of vesicular intermediates in de novo NPC assembly. ..
- Ball H, Ehrhardt M, Mordes D, Glick G, Chazin W, Cortez D. Function of a conserved checkpoint recruitment domain in ATRIP proteins. Mol Cell Biol. 2007;27:3367-77 pubmed..Our results support the idea of a multistep model for ATR activation that requires separable localization and activation functions of ATRIP. ..
- Bendjennat M, Weil P. The transcriptional repressor activator protein Rap1p is a direct regulator of TATA-binding protein. J Biol Chem. 2008;283:8699-710 pubmed publisher..TBP-Rap1p complex formation inhibits TBP binding to TATA promoter DNA. Alterations in either Rap1p or TBP levels modulate mRNA gene transcription in vivo. We propose that Rap1p represents a heretofore unrecognized regulator of TBP. ..
- Warren E, Vaithiyalingam S, Haworth J, Greer B, Bielinsky A, Chazin W, et al. Structural basis for DNA binding by replication initiator Mcm10. Structure. 2008;16:1892-901 pubmed publisher..In addition, mapping Mcm10 mutations known to disrupt PCNA, polymerase alpha, and DNA interactions onto the crystal structure provides insight into how Mcm10 might coordinate protein and DNA binding within the replisome. ..
- Vander Kooi C, Ren L, Xu P, Ohi M, Gould K, Chazin W. The Prp19 WD40 domain contains a conserved protein interaction region essential for its function. Structure. 2010;18:584-93 pubmed publisher..Experiments in vitro and in vivo demonstrate that two molecules of Cwc2 bind to the Prp19 tetramer. These coupled structural and functional studies provide a model for the functional architecture of Prp19. ..
- Chandrasekharan M, Huang F, Sun Z. Decoding the trans-histone crosstalk: methods to analyze H2B ubiquitination, H3 methylation and their regulatory factors. Methods. 2011;54:304-14 pubmed publisher..A quick and easy method to measure overall protein abundance and changes in protein-protein and protein-DNA interactions on native chromatin is also described. ..
- Talley J, Dezwaan D, Maness L, Freeman B, Friedman K. Stimulation of yeast telomerase activity by the ever shorter telomere 3 (Est3) subunit is dependent on direct interaction with the catalytic protein Est2. J Biol Chem. 2011;286:26431-9 pubmed publisher..Additionally, it contributes functional support to the idea that Est3p is structurally related to the mammalian shelterin protein, TPP1, which also influences telomerase activity through interaction with the Est2p homolog, TERT. ..
- Casey A, Dawson T, Chen J, Friederichs J, Jaspersen S, Wente S. Integrity and function of the Saccharomyces cerevisiae spindle pole body depends on connections between the membrane proteins Ndc1, Rtn1, and Yop1. Genetics. 2012;192:441-55 pubmed publisher..We propose that NPC and SPB biogenesis are altered in cells lacking Rtn1 and Yop1 due to competition between these complexes for Ndc1, an essential common component of both NPCs and SPBs...
- Layer J, Weil P. Direct TFIIA-TFIID protein contacts drive budding yeast ribosomal protein gene transcription. J Biol Chem. 2013;288:23273-94 pubmed publisher..These data suggest a mechanism for how interactions between TFIID, TFIIA, and Rap1 contribute to the high rate of transcription initiation seen on RPGs in vivo. ..
- Sugitani N, Voehler M, Roh M, Topolska Woś A, Chazin W. Analysis of DNA binding by human factor xeroderma pigmentosum complementation group A (XPA) provides insight into its interactions with nucleotide excision repair substrates. J Biol Chem. 2017;292:16847-16857 pubmed publisher..These results suggest that for XPA mutants exhibiting altered DNA-binding properties, a correlation exists between the extent of reduction in DNA-binding affinity and the severity of symptoms in XP patients. ..
- Balasubramanian M, Feoktistova A, McCollum D, Gould K. Fission yeast Sop2p: a novel and evolutionarily conserved protein that interacts with Arp3p and modulates profilin function. EMBO J. 1996;15:6426-37 pubmed..Collectively these data demonstrate the interaction of Sop2p with Arp3p, profilin and actin. ..
- Natarajan P, Wang J, Hua Z, Graham T. Drs2p-coupled aminophospholipid translocase activity in yeast Golgi membranes and relationship to in vivo function. Proc Natl Acad Sci U S A. 2004;101:10614-9 pubmed..Therefore, whereas NBD-PS is a preferred substrate for Drs2p in vitro, endogenous PS is not an obligatory substrate in vivo for the role Drs2p plays in protein transport...
- McClendon A, Osheroff N. The geometry of DNA supercoils modulates topoisomerase-mediated DNA cleavage and enzyme response to anticancer drugs. Biochemistry. 2006;45:3040-50 pubmed
- Au C, Benedetto A, Anderson J, Labrousse A, Erikson K, Ewbank J, et al. SMF-1, SMF-2 and SMF-3 DMT1 orthologues regulate and are regulated differentially by manganese levels in C. elegans. PLoS ONE. 2009;4:e7792 pubmed publisher..In sum, we unraveled a complex interplay of transcriptional and post-translational regulations of 3 DMT1-like transporters in two adjacent tissues, which regulate metal-content in C. elegans. ..
- Ohi M, Gould K. Characterization of interactions among the Cef1p-Prp19p-associated splicing complex. RNA. 2002;8:798-815 pubmed..Further, our data indicate that a mutation in the U-box of Prp19p disrupts these numerous protein interactions causing Cef1p degradation and Ntc instability. ..
- AlcÃ¡zar RomÃ¡n A, Bolger T, Wente S. Control of mRNA export and translation termination by inositol hexakisphosphate requires specific interaction with Gle1. J Biol Chem. 2010;285:16683-92 pubmed publisher..We conclude that Gle1 specifically binds IP(6) and that this interaction is required for the full potentiation of Dbp5 ATPase activity during both mRNA export and translation termination. ..
- Sammons M, Samir P, Link A. Saccharomyces cerevisiae Gis2 interacts with the translation machinery and is orthogonal to myotonic dystrophy type 2 protein ZNF9. Biochem Biophys Res Commun. 2011;406:13-9 pubmed publisher..These data suggest that Gis2 is functionally orthologous to ZNF9 and acts as a cap-independent translation factor...
- Cooper J, Liu L, Woodruff E, Taylor H, Goodwin J, D Aquila R, et al. Filamin A protein interacts with human immunodeficiency virus type 1 Gag protein and contributes to productive particle assembly. J Biol Chem. 2011;286:28498-510 pubmed publisher..These results suggest that filamin A is involved in the distinct step of the Gag trafficking pathway. The discovery of the Gag-filamin A interaction may provide a new therapeutic target for the treatment of HIV infection. ..
- Bruck I, Kanter D, Kaplan D. Enabling association of the GINS protein tetramer with the mini chromosome maintenance (Mcm)2-7 protein complex by phosphorylated Sld2 protein and single-stranded origin DNA. J Biol Chem. 2011;286:36414-26 pubmed publisher..Furthermore, origin ssDNA may stimulate the formation of the CMG complex by alleviating inhibitory interactions between Sld2 with Mcm2-7. ..
- Gall W, Higginbotham M, Chen C, Ingram M, Cyr D, Graham T. The auxilin-like phosphoprotein Swa2p is required for clathrin function in yeast. Curr Biol. 2000;10:1349-58 pubmed..They suggest that Swa2p is the yeast ortholog of auxilin and has a role in disassembling clathrin, not only in uncoating clathrin-coated vesicles but perhaps in preventing unproductive clathrin assembly in vivo. ..
- Burns C, Ohi R, Mehta S, O TOOLE E, Winey M, Clark T, et al. Removal of a single alpha-tubulin gene intron suppresses cell cycle arrest phenotypes of splicing factor mutations in Saccharomyces cerevisiae. Mol Cell Biol. 2002;22:801-15 pubmed
- Ji H, Adkins C, Cartwright B, Friedman K. Yeast Est2p affects telomere length by influencing association of Rap1p with telomeric chromatin. Mol Cell Biol. 2008;28:2380-90 pubmed publisher..Taken together, these results suggest that Est2p can directly or indirectly influence the binding of Rap1p to telomeric DNA, implicating telomerase in roles both upstream and downstream of Rap1p in telomere length homeostasis...
- Baldridge R, Graham T. Two-gate mechanism for phospholipid selection and transport by type IV P-type ATPases. Proc Natl Acad Sci U S A. 2013;110:E358-67 pubmed publisher..This work describes a unique transport mechanism for a P-type ATPase and provides insight into how integral membrane proteins can recognize and transport phospholipid substrate across a lipid bilayer. ..
- Zhou X, Sebastian T, Graham T. Auto-inhibition of Drs2p, a yeast phospholipid flippase, by its carboxyl-terminal tail. J Biol Chem. 2013;288:31807-15 pubmed publisher..This work provides the first direct evidence, in a purified system, that a phospholipid flippase is subject to auto-inhibition by its C-terminal tail, which can be relieved by a phosphoinositide to stimulate flippase activity. ..
- Kirchner J, Sanders S, Klebanow E, Weil P. Molecular genetic dissection of TAF25, an essential yeast gene encoding a subunit shared by TFIID and SAGA multiprotein transcription factors. Mol Cell Biol. 2001;21:6668-80 pubmed..The results of these analyses show that TAF25p is comprised of multiple mutable elements which contribute importantly to RNA polymerase II-mediated mRNA gene transcription. ..
- Gerbasi V, Weaver C, Hill S, Friedman D, Link A. Yeast Asc1p and mammalian RACK1 are functionally orthologous core 40S ribosomal proteins that repress gene expression. Mol Cell Biol. 2004;24:8276-87 pubmed..Our data suggest that one of Asc1p's or RACK1's functions is to repress gene expression. ..
- Luo W, Fang H, Green N. Substrate specificity of inner membrane peptidase in yeast mitochondria. Mol Genet Genomics. 2006;275:431-6 pubmed..These results suggest, surprisingly, G-3-P dehydrogenase has switched from Imp1p in yeast to Imp2p in mammals. ..
- Chen S, Wang J, Muthusamy B, Liu K, Zare S, Andersen R, et al. Roles for the Drs2p-Cdc50p complex in protein transport and phosphatidylserine asymmetry of the yeast plasma membrane. Traffic. 2006;7:1503-17 pubmed
- Singleton C. Identification and characterization of the thiamine transporter gene of Saccharomyces cerevisiae. Gene. 1997;199:111-21 pubmed..The level of transporter mRNA was found to be rapidly and dramatically reduced by the addition of thiamine to the growth medium. ..
- Ryan K, McCaffery J, Wente S. The Ran GTPase cycle is required for yeast nuclear pore complex assembly. J Cell Biol. 2003;160:1041-53 pubmed..We propose a model wherein a Ran-mediated vesicular fusion step is required for NPC assembly into intact nuclear envelopes. ..
- Morrell J, Nichols C, Gould K. The GIN4 family kinase, Cdr2p, acts independently of septins in fission yeast. J Cell Sci. 2004;117:5293-302 pubmed..Our data indicate that Cdr2p functions at two positions within the cell at discrete cell cycle stages to influence the timing of mitotic entry and cytokinesis, respectively...
- Enders J, Marasco C, Kole A, Nguyen B, Sevugarajan S, Seale K, et al. Towards monitoring real-time cellular response using an integrated microfluidics-matrix assisted laser desorption ionisation/nanoelectrospray ionisation-ion mobility-mass spectrometry platform. IET Syst Biol. 2010;4:416-27 pubmed publisher
- DeGregori J, Russ A, von Melchner H, Rayburn H, Priyaranjan P, Jenkins N, et al. A murine homolog of the yeast RNA1 gene is required for postimplantation development. Genes Dev. 1994;8:265-76 pubmed..These similarities suggest that Fug1 may be required for developmental changes in RNA processing or chromatin structure prior to gastrulation. ..
- Spiekerkoetter U, Sun B, Khuchua Z, Bennett M, Strauss A. Molecular and phenotypic heterogeneity in mitochondrial trifunctional protein deficiency due to beta-subunit mutations. Hum Mutat. 2003;21:598-607 pubmed..The degree of reduction in thiolase antigen also correlated with the severity of clinical presentation. Although TFP deficiency is highly heterogeneous, there is genotype-phenotype correlation. ..
- Zhou X, Graham T. Reconstitution of phospholipid translocase activity with purified Drs2p, a type-IV P-type ATPase from budding yeast. Proc Natl Acad Sci U S A. 2009;106:16586-91 pubmed publisher..These data demonstrate for the first time the reconstitution of a flippase activity with a purified P4-ATPase. ..
- Baldridge R, Graham T. Identification of residues defining phospholipid flippase substrate specificity of type IV P-type ATPases. Proc Natl Acad Sci U S A. 2012;109:E290-8 pubmed publisher..Modeling of residues involved in substrate selection suggests a novel P-type ATPase transport pathway at the protein/lipid interface and a potential solution to the giant substrate problem. ..
- Baldridge R, Xu P, Graham T. Type IV P-type ATPases distinguish mono- versus diacyl phosphatidylserine using a cytofacial exit gate in the membrane domain. J Biol Chem. 2013;288:19516-27 pubmed publisher..These studies indicate that Dnf1 poorly recognizes diacylated phospholipid and define key substitutions enabling recognition of endogenous PS. ..
- Wanzek K, Schwindt E, Capra J, Paeschke K. Mms1 binds to G-rich regions in Saccharomyces cerevisiae and influences replication and genome stability. Nucleic Acids Res. 2017;45:7796-7806 pubmed publisher..Based on these data we propose a mechanistic model in which Mms1 binds to specific G-rich/G4 motif located on the lagging strand template for DNA replication and supports Pif1 function, DNA replication and genome integrity. ..
- Burke J, Gould K. Molecular cloning and characterization of the Schizosaccharomyces pombe his3 gene for use as a selectable marker. Mol Gen Genet. 1994;242:169-76 pubmed..pombe ars1 sequence into pUC18 for use in transformation of His3--S. pombe strains. New S. pombe strains in which the his3 gene was deleted have also been constructed. ..
- Fang H, Mullins C, Green N. In addition to SEC11, a newly identified gene, SPC3, is essential for signal peptidase activity in the yeast endoplasmic reticulum. J Biol Chem. 1997;272:13152-8 pubmed..Here we report the cloning of the SPC3 gene encoding the homolog to mammalian signal peptidase subunit SPC22/23. We find that Spc3p is also required for cell growth and signal peptidase activity within the yeast endoplasmic reticulum. ..
- Olsen D, Jordan B, Chen D, Wek R, Cavener D. Isolation of the gene encoding the Drosophila melanogaster homolog of the Saccharomyces cerevisiae GCN2 eIF-2alpha kinase. Genetics. 1998;149:1495-509 pubmed..Drosophila and other higher eukaryotes. Despite these differences, the presence of GCN2 in Drosophila suggests at least partial conservation from yeast to multicellular organisms of the mechanisms responding to amino acid deprivation. ..
- Chen J, Lu L, Ohi M, Creamer K, English C, Partridge J, et al. Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation. J Cell Biol. 2011;195:583-93 pubmed publisher..Our findings establish new roles for Cdk1 and the Nsk1-Dlc1 complex in regulating the k-MT interface and chromosome segregation. ..
- Singh M, Bland C, Weil P. Molecular and genetic characterization of a Taf1p domain essential for yeast TFIID assembly. Mol Cell Biol. 2004;24:4929-42 pubmed..These data are consistent with the hypothesis that Taf1p subserves a critical scaffold function within the TFIID complex. The significance of these data with regard to TFIID structure and function is discussed. ..