Genomes and Genes
Experts and Doctors on flow cytometry in Nashville, Tennessee, United States
Locale: Nashville, Tennessee, United States
Topic: flow cytometry
- Hata A, Lybrand T, Breyer R. Identification of determinants of ligand binding affinity and selectivity in the prostaglandin D2 receptor CRTH2. J Biol Chem. 2005;280:32442-51 pubmed..These results provided the first details of the ligand binding pocket of an eicosanoid-binding chemoattractant receptor. ..
- Rollins Smith L, Flajnik M, Blair P, Davis A, Green W. Involvement of thyroid hormones in the expression of MHC class I antigens during ontogeny in Xenopus. Dev Immunol. 1997;5:133-44 pubmed..Differences in the pattern of expression in different sub-populations of cells suggest a complex pattern of regulation of expression of class I antigens during ontogeny. ..
- Fox R, WATSON J, Von Stetina S, McDermott J, Brodigan T, Fukushige T, et al. The embryonic muscle transcriptome of Caenorhabditis elegans. Genome Biol. 2007;8:R188 pubmed..elegans could reveal evolutionarily conserved models of muscle gene function, with ready application to human muscle pathologies. ..
- Thornburg N, Shepherd B, Crowe J. Transforming growth factor beta is a major regulator of human neonatal immune responses following respiratory syncytial virus infection. J Virol. 2010;84:12895-902 pubmed publisher..These data suggest that TGF-? acts as a major regulator of RSV DC-T-cell responses, which could contribute to immunopathology during infancy. ..
- Forrest J, Campbell J, Schelling P, Stehle T, Dermody T. Structure-function analysis of reovirus binding to junctional adhesion molecule 1. Implications for the mechanism of reovirus attachment. J Biol Chem. 2003;278:48434-44 pubmed..These results suggest that reovirus attachment disrupts hJAM1 dimers and highlight similarities between the attachment strategies of reovirus and adenovirus. ..
- Anderson E, Gutierrez D, Kennedy A, Hasty A. Weight cycling increases T-cell accumulation in adipose tissue and impairs systemic glucose tolerance. Diabetes. 2013;62:3180-8 pubmed publisher..These studies indicate that an exaggerated adaptive immune response in AT may contribute to metabolic dysfunction during weight cycling. ..
- Koumangoye R, Sakwe A, Goodwin J, Patel T, Ochieng J. Detachment of breast tumor cells induces rapid secretion of exosomes which subsequently mediate cellular adhesion and spreading. PLoS ONE. 2011;6:e24234 pubmed publisher..Taken together, these data suggest that cellular detachment is accompanied by significant release of exosomes while cellular adhesion and spreading are enhanced by rapid uptake and disposition of exosomes on the cell surface. ..
- McClintock Treep S, Briggs R, Shults K, Flye Blakemore L, Mosse C, Jagasia M, et al. Quantitative assessment of myeloid nuclear differentiation antigen distinguishes myelodysplastic syndrome from normal bone marrow. Am J Clin Pathol. 2011;135:380-5 pubmed publisher..21), but correlation with patient International Prognostic Scoring System scores suggested a trend (P = .07). Flow cytometric assessment of MNDA in myeloid precursors in bone marrow may be useful for the diagnosis of MDS. ..
- Novitskiy S, Csiki I, Huang Y, Johnson D, Harth E, Carbone D, et al. Anti-vascular endothelial growth factor treatment in combination with chemotherapy delays hematopoietic recovery due to decreased proliferation of bone marrow hematopoietic progenitor cells. J Thorac Oncol. 2010;5:1410-5 pubmed publisher..Signaling through both VEGFR1 and VEGFR2 is required for normal reconstitution of the hematopoietic compartment after cytotoxic chemotherapy. ..
- Rock M, Yoder S, Talbot T, Edwards K, Crowe J. Adverse events after smallpox immunizations are associated with alterations in systemic cytokine levels. J Infect Dis. 2004;189:1401-10 pubmed..We also found that particular adverse events correlated with systemic cytokine patterns, which suggests a role for these molecules in the pathogenesis of adverse events. ..
- Hicar M, Chen X, Kalams S, Sojar H, Landucci G, Forthal D, et al. Low frequency of broadly neutralizing HIV antibodies during chronic infection even in quaternary epitope targeting antibodies containing large numbers of somatic mutations. Mol Immunol. 2016;70:94-103 pubmed publisher..The analysis suggests that large numbers of somatic mutations occur in Ab genes encoding HIV Abs in chronically infected individuals in a non-directed, stochastic, manner. ..
- Kim J, Kim J, Saha D, Cao Q, Shyr Y, Choy H. Potential radiation-sensitizing effect of semisynthetic epothilone B in human lung cancer cells. Radiother Oncol. 2003;68:305-13 pubmed..These results demonstrated that BMS-247550 could enhance the effects of radiation in human lung cancer cells both in vitro and in vivo and that a G2/M block and increased apoptosis might be possible explanations for the enhancement. ..
- Ma L, Gao X, Liu L, Xiang Z, Blackwell T, Williams P, et al. CpG oligodeoxynucleotide triggers the liver inflammatory reaction and abrogates spontaneous tolerance. Liver Transpl. 2009;15:915-23 pubmed publisher..These data demonstrate that CpG ODN initiates an inflammatory reaction and abrogates spontaneous tolerance in the liver transplantation mouse model. Liver Transpl 15:915-923, 2009. (c) 2009 AASLD. ..
- Jayagopal A, Halfpenny K, Perez J, Wright D. Hairpin DNA-functionalized gold colloids for the imaging of mRNA in live cells. J Am Chem Soc. 2010;132:9789-96 pubmed publisher..hAuNP are a promising approach for the real-time analysis of mRNA transport and processing in live cells for elucidation of biological processes and disease pathogenesis. ..
- Amara S, Zheng M, Tiriveedhi V. Oleanolic Acid Inhibits High Salt-Induced Exaggeration of Warburg-like Metabolism in Breast Cancer Cells. Cell Biochem Biophys. 2016;74:427-34 pubmed publisher..Taken together, our data indicate that OA efficiently reverses the enhanced Warburg-like metabolism induced by high salt-mediated osmotic stress along with potential application of OA in anti-cancer therapy. ..
- Wiese C, Fleming N, Buehler D, Southard Smith E. A Uchl1-Histone2BmCherry:GFP-gpi BAC transgene for imaging neuronal progenitors. Genesis. 2013;51:852-61 pubmed publisher..The Uchl1-H2BmCherry:GFP-gpi transgene will be a powerful tool for static and live imaging, as well as isolation of viable neural progenitors to investigate processes of autonomic neurogenesis. ..
- Thomas E, Nakamura M, Wienke D, Isacke C, Pozzi A, Liang P. Endo180 binds to the C-terminal region of type I collagen. J Biol Chem. 2005;280:22596-605 pubmed..The interaction between Endo180 and the C-terminal region of type I collagen appears to play an important role in cell-matrix adhesion. ..
- Lee K, Gudapati P, Dragovic S, Spencer C, Joyce S, Killeen N, et al. Mammalian target of rapamycin protein complex 2 regulates differentiation of Th1 and Th2 cell subsets via distinct signaling pathways. Immunity. 2010;32:743-53 pubmed publisher..Collectively, the data uncover vital mTOR-PKC and mTOR-Akt connections in T cell differentiation and reveal distinct pathways by which mTORC2 regulates development of Th1 and Th2 cell subsets. ..
- Chen Y, Sternberg P, Cai J. Characterization of a Bcl-XL-interacting protein FKBP8 and its splice variant in human RPE cells. Invest Ophthalmol Vis Sci. 2008;49:1721-7 pubmed publisher..The transcriptional activity of NFAT was not affected by FKBP8. FKBP8 and its novel splice variant are Bcl-XL-interacting proteins and regulate the apoptotic signaling pathways in the RPE. ..
- Rezaei K, Chen Y, Cai J, Sternberg P. Modulation of Nrf2-dependent antioxidant functions in the RPE by Zip2, a zinc transporter protein. Invest Ophthalmol Vis Sci. 2008;49:1665-70 pubmed publisher..RPE cells can actively uptake zinc through the transporter Zip2, and the increased intracellular zinc upregulates the Nrf2-dependent antioxidant function. ..
- Cox R, Livesay S, Johnson M, Ohi M, Williams J. The human metapneumovirus fusion protein mediates entry via an interaction with RGD-binding integrins. J Virol. 2012;86:12148-60 pubmed publisher..These results suggest a stepwise mechanism of HMPV entry mediated by the F protein through its interactions with cellular receptors, including RGD-binding integrins...
- Yang J, Kantrow S, Sai J, Hawkins O, Boothby M, Ayers G, et al. INK4a/ARF [corrected] inactivation with activation of the NF-?B/IL-6 pathway is sufficient to drive the development and growth of angiosarcoma. Cancer Res. 2012;72:4682-95 pubmed publisher..Our findings offer clinical implications for targeting the NF-kB/IL-6/STAT3 pathway as a rational strategy to treat angiosarcoma. ..
- Fox R, Von Stetina S, Barlow S, Shaffer C, Olszewski K, Moore J, et al. A gene expression fingerprint of C. elegans embryonic motor neurons. BMC Genomics. 2005;6:42 pubmed..These methods can now be applied to generate a gene expression map of the C. elegans nervous system. ..
- Martin M, Fingleton B, Lynch C, Wells S, McIntyre J, Piston D, et al. Establishment and quantitative imaging of a 3D lung organotypic model of mammary tumor outgrowth. Clin Exp Metastasis. 2008;25:877-85 pubmed publisher..In summary, we have developed a novel in vitro model of breast tumor cells in a common metastatic site that can be used to study tumor/host interactions in an important microenvironment. ..
- Wahlstrom J, Fowler M, Nicholson W, Kovacs W. A novel mutation in the preprovasopressin gene identified in a kindred with autosomal dominant neurohypophyseal diabetes insipidus. J Clin Endocrinol Metab. 2004;89:1963-8 pubmed
- Alfaro M, Vincent A, Saraswati S, Thorne C, Hong C, Lee E, et al. sFRP2 suppression of bone morphogenic protein (BMP) and Wnt signaling mediates mesenchymal stem cell (MSC) self-renewal promoting engraftment and myocardial repair. J Biol Chem. 2010;285:35645-53 pubmed publisher..This work provides important new insight into the mechanisms by which sFRP2 increases MSC self-renewal leading to superior tissue engraftment and enhanced wound healing. ..
- Wilson C, Elizer S, Marshall A, Stocks B, Moore D. Regulation of B lymphocyte responses to Toll-like receptor ligand binding during diabetes prevention in non-obese diabetic (NOD) mice. J Diabetes. 2016;8:120-31 pubmed publisher..These activated B cells mediated TLR3-induced diabetes protection. Immunotherapies must account for both B cell location and activation, and these properties may differ in autoimmune and healthy settings. ..
- Taylor H, Khatua A, Popik W. The innate immune factor apolipoprotein L1 restricts HIV-1 infection. J Virol. 2014;88:592-603 pubmed publisher..Deciphering the precise mechanism of APOL1-mediated HIV-1 restriction may facilitate the design of unique therapeutics to target HIV-1 replication. ..
- Peng D, Belkhiri A, Hu T, Chaturvedi R, Asim M, Wilson K, et al. Glutathione peroxidase 7 protects against oxidative DNA damage in oesophageal cells. Gut. 2012;61:1250-60 pubmed publisher..01). The dysfunction of GPX7 in oesophageal cells increases the levels of ROS and oxidative DNA damage, which are common risk factors for Barrett's oesophagus and OAC. ..
- Ryzhov S, Novitskiy S, Goldstein A, Biktasova A, Blackburn M, Biaggioni I, et al. Adenosinergic regulation of the expansion and immunosuppressive activity of CD11b+Gr1+ cells. J Immunol. 2011;187:6120-9 pubmed publisher..We propose that generation of adenosine by CD73 expressed at high levels on granulocytic MDSCs may promote their expansion and facilitate their immunosuppressive activity. ..
- Muraoka R, Lenferink A, Law B, Hamilton E, Brantley D, Roebuck L, et al. ErbB2/Neu-induced, cyclin D1-dependent transformation is accelerated in p27-haploinsufficient mammary epithelial cells but impaired in p27-null cells. Mol Cell Biol. 2002;22:2204-19 pubmed..These results suggest that p27(+/-) mammary epithelium may be more susceptible to oncogene-induced tumorigenesis, whereas p27-null glands, due to severely impaired cyclin D1/Cdk4 function, are more resistant to transformation. ..
- Li M, Page McCaw P, Chen W. FGF1 Mediates Overnutrition-Induced Compensatory Î²-Cell Differentiation. Diabetes. 2016;65:96-109 pubmed publisher..Thus, the recently discovered antidiabetes function of FGF1 may act partially through increasing Î²-cell differentiation. ..
- Dar A, Zaika A, Piazuelo M, Correa P, Koyama T, Belkhiri A, et al. Frequent overexpression of Aurora Kinase A in upper gastrointestinal adenocarcinomas correlates with potent antiapoptotic functions. Cancer. 2008;112:1688-98 pubmed publisher..Study results indicated that AURKA provides potent antiapoptotic properties to gastrointestinal cells by regulating levels of p53 through the AKT/HDM2 axis. ..
- Taylor H, Linde M, Khatua A, Popik W, Hildreth J. Sterol regulatory element-binding protein 2 couples HIV-1 transcription to cholesterol homeostasis and T cell activation. J Virol. 2011;85:7699-709 pubmed publisher..Collectively, our results demonstrate for the first time that HIV-1 transcription in T cells is linked to cholesterol homeostasis through control of TFII-I expression by SREBP2. ..
- Zaynagetdinov R, Sherrill T, Polosukhin V, Han W, Ausborn J, McLoed A, et al. A critical role for macrophages in promotion of urethane-induced lung carcinogenesis. J Immunol. 2011;187:5703-11 pubmed publisher..Together, these studies elucidate an important role for macrophages in lung tumor promotion and indicate that these cells have distinct roles during different stages of lung carcinogenesis...
- Lin Q, Liu Y, Moore D, Elizer S, Veach R, Hawiger J, et al. Cutting edge: the "death" adaptor CRADD/RAIDD targets BCL10 and suppresses agonist-induced cytokine expression in T lymphocytes. J Immunol. 2012;188:2493-7 pubmed publisher..These results define a novel role for CRADD as a negative regulator of the CARMA1 signalosome and suppressor of Th1- and Th17-mediated inflammatory responses. ..
- Olivares Villagómez D, Algood H, Singh K, Parekh V, Ryan K, Piazuelo M, et al. Intestinal epithelial cells modulate CD4 T cell responses via the thymus leukemia antigen. J Immunol. 2011;187:4051-60 pubmed publisher..Overall, our results provide evidence for an important interaction between IEC and CD4(+) T cells via TL, which modulates mucosal immune responses. ..
- Williams J, Weitkamp J, Blum D, Lafleur B, Crowe J. The human neonatal B cell response to respiratory syncytial virus uses a biased antibody variable gene repertoire that lacks somatic mutations. Mol Immunol. 2009;47:407-14 pubmed publisher..These biased features of the RSV-specific repertoire likely contribute to the poor functional Ab response in very young infants. ..
- Zhao J, Hedera P. Hereditary spastic paraplegia-causing mutations in atlastin-1 interfere with BMPRII trafficking. Mol Cell Neurosci. 2013;52:87-96 pubmed publisher..This, together with previously demonstrated inhibition of atlastin-1 of BMP pathway, further supports the role of this signaling cascade in axonal maintenance and axonal degeneration, which is seen in various types of HSP. ..
- Deskins D, Bastakoty D, Saraswati S, Shinar A, Holt G, Young P. Human mesenchymal stromal cells: identifying assays to predict potency for therapeutic selection. Stem Cells Transl Med. 2013;2:151-8 pubmed publisher..These findings suggest that reliable and reproducible in vitro assays may be used to measure the functional potential of MSCs for therapeutic use. ..
- Roh M, Franco O, Hayward S, Van Der Meer R, Abdulkadir S. A role for polyploidy in the tumorigenicity of Pim-1-expressing human prostate and mammary epithelial cells. PLoS ONE. 2008;3:e2572 pubmed publisher..Remarkably, only the sorted polyploid cells were tumorigenic in vitro and in vivo. Our results support the notion that polyploidy can promote chromosomal instability and the initiation of tumorigenesis in human epithelial cells. ..
- Dar A, Belkhiri A, el Rifai W. The aurora kinase A regulates GSK-3beta in gastric cancer cells. Oncogene. 2009;28:866-75 pubmed publisher..We conclude that the AURKA/GSK-3beta interaction is important in regulating beta-catenin, underscoring a novel oncogenic potential for AURKA in gastric tumorigenesis. ..
- Lewis N, Asim M, Barry D, Singh K, de Sablet T, Boucher J, et al. Arginase II restricts host defense to Helicobacter pylori by attenuating inducible nitric oxide synthase translation in macrophages. J Immunol. 2010;184:2572-82 pubmed publisher..These studies implicate Arg2 in the immune evasion of H. pylori by causing intracellular depletion of l-arginine and thus reduction of NO-dependent bactericidal activity. ..
- Fu C, Cawthon B, Clinkscales W, Bruce A, Winzenburger P, Ess K. GABAergic interneuron development and function is modulated by the Tsc1 gene. Cereb Cortex. 2012;22:2111-9 pubmed publisher..These findings support an important role for the Tsc1 gene during GABAergic interneuron development, function, and possibly migration. ..
- Potter L, Choi E, Hipkens S, Wright C, Magnuson M. A recombinase-mediated cassette exchange-derived cyan fluorescent protein reporter allele for Pdx1. Genesis. 2012;50:384-92 pubmed publisher..The Pdx1(CFP) allele will enable the isolation of specific foregut endoderm and pancreatic cell populations, both alone and in combination with other FP reporter alleles. ..
- Horvath D, Radin J, Cho S, Washington M, Algood H. The interleukin-17 receptor B subunit is essential for the Th2 response to Helicobacter pylori, but not for control of bacterial burden. PLoS ONE. 2013;8:e60363 pubmed publisher..These data indicate that signaling through the IL-17 receptor B subunit is not necessary for control of Helicobacter pylori in our model. ..
- Moore M, Newcomb D, Parekh V, Van Kaer L, Collins R, Zhou W, et al. STAT1 negatively regulates lung basophil IL-4 expression induced by respiratory syncytial virus infection. J Immunol. 2009;183:2016-26 pubmed publisher..Basophils were the primary source of IL-4 in the lung in RSV infection, and STAT1 was a negative regulator of virus-induced basophil IL-4 expression. ..
- Parker M, Roberts R, Enriquez M, Zhao X, Takahashi T, Pat Cerretti D, et al. Reverse endocytosis of transmembrane ephrin-B ligands via a clathrin-mediated pathway. Biochem Biophys Res Commun. 2004;323:17-23 pubmed..These results suggest that ephrin-B1 internalization is an active receptor-mediated process that utilizes the clathrin-mediated endocytic pathway. ..
- Huang Y, Chen X, Dikov M, Novitskiy S, Mosse C, Yang L, et al. Distinct roles of VEGFR-1 and VEGFR-2 in the aberrant hematopoiesis associated with elevated levels of VEGF. Blood. 2007;110:624-31 pubmed..Our findings suggest that selective blockade of VEGFR-2 rather than of both receptors may optimally overcome the adverse hematologic consequences of elevated VEGF levels found in malignancy. ..
- Song C, Aiken C. Analysis of human cell heterokaryons demonstrates that target cell restriction of cyclosporine-resistant human immunodeficiency virus type 1 mutants is genetically dominant. J Virol. 2007;81:11946-56 pubmed..Collectively, these results support the existence of a cell-specific human cellular factor capable of restricting HIV-1 at an early post-entry step by a CypA-dependent mechanism. ..
- Toki S, Goleniewska K, Huckabee M, Zhou W, Newcomb D, Fitzgerald G, et al. PGI? signaling inhibits antigen uptake and increases migration of immature dendritic cells. J Leukoc Biol. 2013;94:77-88 pubmed publisher..Our results suggest PGI2-IP signaling increases immature DC migration to the draining LNs and may represent a novel mechanism by which this eicosanoid inhibits immune responses. ..
- Lee K, Heffington L, Jellusova J, Nam K, Raybuck A, Cho S, et al. Requirement for Rictor in homeostasis and function of mature B lymphoid cells. Blood. 2013;122:2369-79 pubmed publisher..Collectively, the findings provide evidence that mTOR signaling affects survival and proliferation of mature B lymphocytes, and establish Rictor as an important signal relay in B-cell homeostasis, fate, and functions. ..
- Vigerust D, Egan B, Shepherd V. HIV-1 Nef mediates post-translational down-regulation and redistribution of the mannose receptor. J Leukoc Biol. 2005;77:522-34 pubmed..These data suggest that the MR is a potential intracellular target of Nef and that this regulation may represent a mechanism to further cripple the host innate immune system. ..
- Volanakis E, Boothby M, Sherr C. Epigenetic regulation of the Ink4a-Arf (Cdkn2a) tumor suppressor locus in the initiation and progression of Notch1-driven T cell acute lymphoblastic leukemia. Exp Hematol. 2013;41:377-86 pubmed publisher..Epigenetic remodeling during tumor progression licenses Arf as a tumor suppressor and in turn provides the selective pressure for Ink4a-Arf deletion in clonal T-ALLs that emerge. ..
- Stathopoulos G, Sherrill T, Karabela S, Goleniewska K, Kalomenidis I, Roussos C, et al. Host-derived interleukin-5 promotes adenocarcinoma-induced malignant pleural effusion. Am J Respir Crit Care Med. 2010;182:1273-81 pubmed publisher..Because eosinophils can be found in malignant pleural effusions (MPE) from mice and humans, we asked whether IL-5 is involved in the pathogenesis of MPE...
- Nicholas K, Greenplate A, Flaherty D, Matlock B, Juan J, Smith R, et al. Multiparameter analysis of stimulated human peripheral blood mononuclear cells: A comparison of mass and fluorescence cytometry. Cytometry A. 2016;89:271-80 pubmed publisher..These results suggest combinations of unsupervised analysis programs and extended multiparameter cytometry will be indispensable tools for detecting perturbations in protein expression in both health and disease. ..
- Wu L, Parekh V, Gabriel C, Bracy D, Marks Shulman P, TAMBOLI R, et al. Activation of invariant natural killer T cells by lipid excess promotes tissue inflammation, insulin resistance, and hepatic steatosis in obese mice. Proc Natl Acad Sci U S A. 2012;109:E1143-52 pubmed publisher..These findings place iNKT cells into the complex network linking lipid excess to inflammation in obesity and suggest new therapeutic avenues for obesity-associated metabolic disorders. ..
- Stein S, Thomas E, Herzog B, Westfall M, Rocheleau J, Jackson R, et al. NDRG1 is necessary for p53-dependent apoptosis. J Biol Chem. 2004;279:48930-40 pubmed..This report further supports the notion that p53 controls a network of genes that are required for its apoptotic function. ..
- Newcomb D, Boswell M, Zhou W, Huckabee M, Goleniewska K, Sevin C, et al. Human TH17 cells express a functional IL-13 receptor and IL-13 attenuates IL-17A production. J Allergy Clin Immunol. 2011;127:1006-13.e1-4 pubmed publisher..Although IL-13 is an attractive therapeutic target for decreasing symptoms associated with asthma, these results suggest that therapies inhibiting IL-13 production could have adverse side effects by increasing IL-17A production. ..
- Yu S, Lau C, Barham W, Onishko H, Nelson C, Li H, et al. Macrophage-specific RNA interference targeting via "click", mannosylated polymeric micelles. Mol Pharm. 2013;10:975-87 pubmed publisher..This work also establishes a generalizable platform that could be applied for "click" functionalization with other targeting ligands to direct siRNA delivery. ..
- Tian C, Luskin G, Dischert K, Higginbotham J, Shepherd B, Crowe J. Evidence for preferential Ig gene usage and differential TdT and exonuclease activities in human naïve and memory B cells. Mol Immunol. 2007;44:2173-83 pubmed..These data suggest distinct functional mechanisms underlying selection of this unique subset of un-class-switched memory B cells. ..
- Wei J, O BRIEN D, Vilgelm A, Piazuelo M, Correa P, Washington M, et al. Interaction of Helicobacter pylori with gastric epithelial cells is mediated by the p53 protein family. Gastroenterology. 2008;134:1412-23 pubmed publisher..Our findings implicate p73 in H pylori-induced apoptosis and more generally suggest that the p53 family may play a role in the epithelial cell response to H pylori infection. ..