Genomes and Genes
Experts and Doctors on drosophila melanogaster in Providence, Rhode Island, United States
Locale: Providence, Rhode Island, United States
Topic: drosophila melanogaster
- Bearer E. Distribution of Xrel in the early Xenopus embryo: a cytoplasmic and nuclear gradient. Eur J Cell Biol. 1994;63:255-68 pubmed..The presence of this putative transcription factor in the nuclei of these cells prior to mid-blastula transition suggests that Xrel-1 may be involved in programming animal cells to respond to vegetal-inducing factors. ..
- Soruco M, Chery J, Bishop E, Siggers T, Tolstorukov M, Leydon A, et al. The CLAMP protein links the MSL complex to the X chromosome during Drosophila dosage compensation. Genes Dev. 2013;27:1551-6 pubmed publisher..The discovery of CLAMP identifies a key factor required for the chromosome-specific targeting of dosage compensation, providing new insights into how subnuclear domains of coordinate gene regulation are formed within metazoan genomes. ..
- Fridell Y, Hoh M, Kréneisz O, Hosier S, Chang C, Scantling D, et al. Increased uncoupling protein (UCP) activity in Drosophila insulin-producing neurons attenuates insulin signaling and extends lifespan. Aging (Albany NY). 2009;1:699-713 pubmed..In summary, we have demonstrated a role for UCP in adult Drosophila IPCs in influencing systemic insulin signaling and longevity by a mechanism that may involve K(ATP) channels...
- Rand D, Kann L. Mutation and selection at silent and replacement sites in the evolution of animal mitochondrial DNA. Genetica. 1998;102-103:393-407 pubmed..An important challenge is to tease apart the impact of mutation and selection on levels of polymorphism versus divergence in a genome that does not generally recombine. ..
- Bai H, Kang P, Hernandez A, Tatar M. Activin signaling targeted by insulin/dFOXO regulates aging and muscle proteostasis in Drosophila. PLoS Genet. 2013;9:e1003941 pubmed publisher..Reduced insulin secretion from the brain may subsequently reinforce longevity assurance through decreased systemic insulin/IGF signaling. ..
- Jepson J, Savva Y, Jay K, Reenan R. Visualizing adenosine-to-inosine RNA editing in the Drosophila nervous system. Nat Methods. 2011;9:189-94 pubmed publisher..Our data suggest variable RNA editing as a credible molecular mechanism for mediating individual-to-individual variation in neuronal physiology and behavior. ..
- Whitaker R, Faulkner S, Miyokawa R, Burhenn L, Henriksen M, Wood J, et al. Increased expression of Drosophila Sir2 extends life span in a dose-dependent manner. Aging (Albany NY). 2013;5:682-91 pubmed..Our results help to resolve the apparently conflicting reports by demonstrating that the effects of increased dSir2 expression on life span in Drosophila are dependent upon dSir2 dosage. ..
- Flatt T, Min K, D Alterio C, Villa Cuesta E, Cumbers J, Lehmann R, et al. Drosophila germ-line modulation of insulin signaling and lifespan. Proc Natl Acad Sci U S A. 2008;105:6368-73 pubmed publisher..These results suggest that signals from the gonad regulate lifespan and modulate insulin sensitivity in the fly and that the gonadal regulation of aging is evolutionarily conserved. ..
- Shirangi T, Taylor B, McKeown M. A double-switch system regulates male courtship behavior in male and female Drosophila melanogaster. Nat Genet. 2006;38:1435-9 pubmed..Thus, we show that fru and dsx together act as a 'switch' system regulating behavior in the context of other developmental genes, such as retn. ..
- Zerofsky M, Harel E, Silverman N, Tatar M. Aging of the innate immune response in Drosophila melanogaster. Aging Cell. 2005;4:103-8 pubmed..Consequently, maximum reproduction will occur when the immune response is tightly controlled in young females, even if this increases infection risk at later ages. ..
- Bangi E, Wharton K. Dpp and Gbb exhibit different effective ranges in the establishment of the BMP activity gradient critical for Drosophila wing patterning. Dev Biol. 2006;295:178-93 pubmed..The existence of related ligands with different functional ranges may represent a conserved mechanism used in different species to generate robust long range activity gradients. ..
- Staber C, Gell S, Jepson J, Reenan R. Perturbing A-to-I RNA editing using genetics and homologous recombination. Methods Mol Biol. 2011;718:41-73 pubmed publisher..These new approaches promise to significantly improve our understanding of how mRNA modification contributes to insect physiology and ethology. ..
- Bergland A, Genissel A, Nuzhdin S, Tatar M. Quantitative trait loci affecting phenotypic plasticity and the allometric relationship of ovariole number and thorax length in Drosophila melanogaster. Genetics. 2008;180:567-82 pubmed publisher..The results are discussed in the context of multivariate trait evolution...
- Zimmering S, Cruces M, Pimentel E, Arceo C, Carrasco G, Olvera O. On the recovery of single spots with the flr phenotype in the wing spot test in Drosophila. Mutat Res. 1997;379:77-82 pubmed..An argument is presented questioning the use of treated mwh +/+ TM3 individuals as an assay of mutations/deletions at the mwh+ locus. ..
- Savva Y, Jepson J, Chang Y, Whitaker R, Jones B, St Laurent G, et al. RNA editing regulates transposon-mediated heterochromatic gene silencing. Nat Commun. 2013;4:2745 pubmed publisher..Lastly, systemic differences in RNA editing activity generates interindividual variation in silencing state within a population. Our data reveal a global role for RNA editing in regulating gene expression. ..
- Jiang N, Du G, Tobias E, Wood J, Whitaker R, Neretti N, et al. Dietary and genetic effects on age-related loss of gene silencing reveal epigenetic plasticity of chromatin repression during aging. Aging (Albany NY). 2013;5:813-24 pubmed
- Tatar M, Yin C. Slow aging during insect reproductive diapause: why butterflies, grasshoppers and flies are like worms. Exp Gerontol. 2001;36:723-38 pubmed..elegans daf-2. We propose neuroendocrine control of reproductive diapause in D. melanogaster that includes phenotypic plasticity for rates of senescence. ..
- Jepson J, Savva Y, Yokose C, Sugden A, Sahin A, Reenan R. Engineered alterations in RNA editing modulate complex behavior in Drosophila: regulatory diversity of adenosine deaminase acting on RNA (ADAR) targets. J Biol Chem. 2011;286:8325-37 pubmed publisher..Our data demonstrate that network-wide temporal and spatial regulation of ADAR activity can tune the complex system of RNA-editing sites and modulate multiple ethologically relevant behavioral modalities. ..
- Wang P, Neretti N, Whitaker R, Hosier S, Chang C, Lu D, et al. Long-lived Indy and calorie restriction interact to extend life span. Proc Natl Acad Sci U S A. 2009;106:9262-7 pubmed publisher..We conclude that Indy and CR interact to affect longevity and that a decrease in Indy may induce a CR-like status that confers life span extension. ..
- Garbuzov A, Tatar M. Hormonal regulation of Drosophila microRNA let-7 and miR-125 that target innate immunity. Fly (Austin). 2010;4:306-11 pubmed..We conclude that 20-HE facilitates the initial expression of innate immunity while it simultaneously induces negative regulation via microRNA control of antimicrobial peptide translation. ..
- Rand D, Weinreich D, Lerman D, Folk D, Gilchrist G. Three selections are better than one: clinal variation of thermal QTL from independent selection experiments in Drosophila. Evolution. 2010;64:2921-34 pubmed publisher..Together, these studies show that independent selection experiments can uncover the same target of selection and that evolution in the laboratory can recapitulate putatively adaptive clinal variation in nature. ..
- Flatt T, Kawecki T. Pleiotropic effects of methoprene-tolerant (Met), a gene involved in juvenile hormone metabolism, on life history traits in Drosophila melanogaster. Genetica. 2004;122:141-60 pubmed..The allelic effects of Met support genetic models where pleiotropy at genes associated with hormone regulation can contribute to the evolution of life history traits. ..
- Savva Y, Jepson J, Sahin A, Sugden A, Dorsky J, Alpert L, et al. Auto-regulatory RNA editing fine-tunes mRNA re-coding and complex behaviour in Drosophila. Nat Commun. 2012;3:790 pubmed publisher..Our results reveal the in vivo relevance of auto-regulatory control over post-transcriptional mRNA re-coding events in fine-tuning brain function and organismal behaviour. ..
- Flatt T, Tu M, Tatar M. Hormonal pleiotropy and the juvenile hormone regulation of Drosophila development and life history. Bioessays. 2005;27:999-1010 pubmed..In particular, we illustrate the role of JH as a key mediator of life history trade-offs. ..
- Ditch L, Shirangi T, Pitman J, Latham K, Finley K, Edeen P, et al. Drosophila retained/dead ringer is necessary for neuronal pathfinding, female receptivity and repression of fruitless independent male courtship behaviors. Development. 2005;132:155-64 pubmed..We posit that some of these retn-expressing cells function to repress a male behavioral pathway activated by fruM. ..
- Hwangbo D, Gershman B, Gersham B, Tu M, Palmer M, Tatar M. Drosophila dFOXO controls lifespan and regulates insulin signalling in brain and fat body. Nature. 2004;429:562-6 pubmed..These findings suggest that autonomous and non-autonomous roles of insulin signalling combine to control ageing. ..
- Kaye E, Kurbidaeva A, Wolle D, Aoki T, Schedl P, Larschan E. Drosophila Dosage Compensation Loci Associate with a Boundary-Forming Insulator Complex. Mol Cell Biol. 2017;37: pubmed publisher..Moreover, reducing the levels of two LBC components compromises MSL recruitment. Finally, we show that several of the CES that are physically linked to each other in vivo are LBC interactors. ..
- Montooth K, Meiklejohn C, Abt D, Rand D. Mitochondrial-nuclear epistasis affects fitness within species but does not contribute to fixed incompatibilities between species of Drosophila. Evolution. 2010;64:3364-79 pubmed publisher..We propose that a low mitochondrial substitution rate, resulting from a low mutation rate and/or efficient purifying selection, precludes the accumulation of mitochondrial-nuclear incompatibilities among these Drosophila species. ..
- Rand D, Dorfsman M, Kann L. Neutral and non-neutral evolution of Drosophila mitochondrial DNA. Genetics. 1994;138:741-56 pubmed..The data reveal how different rates of mtDNA evolution between species and different histories of neutral and adaptive evolution within species can compromise historical inferences in population and evolutionary biology. ..
- Antosh M, Fox D, Helfand S, Cooper L, Neretti N. New comparative genomics approach reveals a conserved health span signature across species. Aging (Albany NY). 2011;3:576-83 pubmed..Our approach can thus be used to explore and better define the relationships between highly complex biological phenomena, in this case those that affect the health and longevity. ..
- Le V, Wharton K. Hyperactive BMP signaling induced by ALK2(R206H) requires type II receptor function in a Drosophila model for classic fibrodysplasia ossificans progressiva. Dev Dyn. 2012;241:200-14 pubmed publisher..The mechanism(s) regulating the expressivity of hyperactive ALK2(R206H) signaling throughout a patient's life is not well understood...
- Bergland A, Chae H, Kim Y, Tatar M. Fine-scale mapping of natural variation in fly fecundity identifies neuronal domain of expression and function of an aquaporin. PLoS Genet. 2012;8:e1002631 pubmed publisher..Taken together these data suggest that natural variation in Drip expression in the corazonin producing neurons contributes to standing variation in fitness by altering the concentration of two neurohormones. ..
- Ballard S, Jarolimova J, Wharton K. Gbb/BMP signaling is required to maintain energy homeostasis in Drosophila. Dev Biol. 2010;337:375-85 pubmed publisher..Overall, our results implicate Gbb/BMP signaling as a new pathway critical for positive regulation of nutrient storage and energy homeostasis during development. ..
- Gershman B, Puig O, Hang L, Peitzsch R, Tatar M, Garofalo R. High-resolution dynamics of the transcriptional response to nutrition in Drosophila: a key role for dFOXO. Physiol Genomics. 2007;29:24-34 pubmed
- Rieder L, Koreski K, Boltz K, Kuzu G, Urban J, Bowman S, et al. Histone locus regulation by the Drosophila dosage compensation adaptor protein CLAMP. Genes Dev. 2017;31:1494-1508 pubmed publisher..Therefore, CLAMP binding to GA repeat motifs promotes the formation of two distinct domains of coordinated gene activation located at different places in the genome. ..
- Silbermann R, Tatar M. Reproductive costs of heat shock protein in transgenic Drosophila melanogaster. Evolution. 2000;54:2038-45 pubmed..The contervailing effects of hsp70 upon fecundity and subsequent age-specific mortality exemplify antagonistic pleiotropy, and this trade-off could contribute to the evolution of Drosophila senescence. ..
- Flatt T, Heyland A, Rus F, Porpiglia E, Sherlock C, Yamamoto R, et al. Hormonal regulation of the humoral innate immune response in Drosophila melanogaster. J Exp Biol. 2008;211:2712-24 pubmed publisher..Our results suggest that 20E and JH play major roles in the regulation of gene expression in response to immune challenge. ..
- Bauer J, Chang C, Morris S, Hozier S, Andersen S, Waitzman J, et al. Expression of dominant-negative Dmp53 in the adult fly brain inhibits insulin signaling. Proc Natl Acad Sci U S A. 2007;104:13355-60 pubmed
- Tatar M. The plate half-full: status of research on the mechanisms of dietary restriction in Drosophila melanogaster. Exp Gerontol. 2011;46:363-8 pubmed publisher..Strong analyses of genes required for DR should be a priority in future research with Drosophila and this may be made most robust by considering the effect of mutants in the context of the geometric framework. ..
- Yamamoto R, Bai H, Dolezal A, Amdam G, Tatar M. Juvenile hormone regulation of Drosophila aging. BMC Biol. 2013;11:85 pubmed publisher..We identify transcripts that change in response to juvenile hormone independent of reproductive state and suggest these represent somatically expressed genes that could modulate how juvenile hormone controls persistence and longevity. ..
- Montooth K, Siebenthall K, Clark A. Membrane lipid physiology and toxin catabolism underlie ethanol and acetic acid tolerance in Drosophila melanogaster. J Exp Biol. 2006;209:3837-50 pubmed
- Tu M, Tatar M. Juvenile diet restriction and the aging and reproduction of adult Drosophila melanogaster. Aging Cell. 2003;2:327-33 pubmed..Although nutritional conditions of the larvae can affect the subsequent body size and fecundity of adults, these are not sufficient to slow aging. ..
- Koveal D, Schuh Nuhfer N, Ritt D, Page R, Morrison D, Peti W. A CC-SAM, for coiled coil-sterile ? motif, domain targets the scaffold KSR-1 to specific sites in the plasma membrane. Sci Signal. 2012;5:ra94 pubmed publisher..Thus, in addition to the atypical C1 domain, the CC-SAM domain is required to target KSR-1 to the plasma membrane. ..
- Zimmering S, Osgood C, Mason J. Aneuploidy in Drosophila, I. Genetic test systems in the female Drosophila melanogaster for the rapid detection of chemically induced chromosome gain and chromosome loss. Mutat Res. 1990;234:319-26 pubmed..Consequently, detection of compounds with weak effects requiring large sample sizes may be made in a fraction of the time associated with more traditional schemes for aneuploidy detection in Drosophila. ..
- Pitman J, Tsai C, Edeen P, Finley K, Evans R, McKeown M. DSF nuclear receptor acts as a repressor in culture and in vivo. Dev Biol. 2002;245:315-28 pubmed..These results suggest DSF can repress transcription in vivo, that repression is largely responsible for its ectopic expression phenotypes, and that repression may be a key component of normal DSF function. ..
- Tu M, Epstein D, Tatar M. The demography of slow aging in male and female Drosophila mutant for the insulin-receptor substrate homologue chico. Aging Cell. 2002;1:75-80 pubmed..Remarkably, in similarly sized cohorts of male and female ch1/ch1 and of male ch1/+ mortality deceleration is absent. Mortality deceleration is a phenotype of chico. ..
- Rieder L, Staber C, Hoopengardner B, Reenan R. Tertiary structural elements determine the extent and specificity of messenger RNA editing. Nat Commun. 2013;4:2232 pubmed publisher..Our results demonstrate that complex RNA tertiary structures, which may be difficult to predict computationally, form in vivo and can regulate RNA-editing events. ..
- Bai H, Kang P, Tatar M. Drosophila insulin-like peptide-6 (dilp6) expression from fat body extends lifespan and represses secretion of Drosophila insulin-like peptide-2 from the brain. Aging Cell. 2012;11:978-85 pubmed publisher..dilp6 thus appears to bridge dFOXO, adipose tissue and brain endocrine function to regulate Drosophila longevity...
- Neretti N, Wang P, Brodsky A, Nyguyen H, White K, Rogina B, et al. Long-lived Indy induces reduced mitochondrial reactive oxygen species production and oxidative damage. Proc Natl Acad Sci U S A. 2009;106:2277-82 pubmed publisher..Thus, one potential mechanism by which Indy mutants extend life span could be through an alteration in mitochondrial physiology leading to an increased efficiency in the ATP/ROS ratio. ..
- Tatar M. Can we develop genetically tractable models to assess healthspan (rather than life span) in animal models?. J Gerontol A Biol Sci Med Sci. 2009;64:161-3 pubmed publisher..It was the consensus of the working session that making healthspan an operational metric would be an innovation needed for the genetic power of model systems to address this aspect of human aging. ..