Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae proteins in Philadelphia, Pennsylvania, United States
Locale: Philadelphia, Pennsylvania, United States
Topic: saccharomyces cerevisiae proteins
Publications205 found, 100 shown here
- Candau R, Zhou J, Allis C, Berger S. Histone acetyltransferase activity and interaction with ADA2 are critical for GCN5 function in vivo. EMBO J. 1997;16:555-65 pubmed..These results suggest that both functions of GCN5, HAT activity and interaction with ADA2, are necessary for targeting and acetylation of nucleosomal histones. ..
- Tang L, Li J, Katz D, Feng J. Determining the DNA bending angle induced by non-specific high mobility group-1 (HMG-1) proteins: a novel method. Biochemistry. 2000;39:3052-60 pubmed..We believe that this experimental design provides an effective vehicle to determine the DNA bending induced by nonspecific HMG-1 proteins. ..
- Petruk S, Sedkov Y, Smith S, Tillib S, Kraevski V, Nakamura T, et al. Trithorax and dCBP acting in a complex to maintain expression of a homeotic gene. Science. 2001;294:1331-4 pubmed..Thus Trx, dCBP, and Sbf1 are closely linked, physically and functionally, in the maintenance of Hox gene expression. ..
- He B, Xi F, Zhang X, Zhang J, Guo W. Exo70 interacts with phospholipids and mediates the targeting of the exocyst to the plasma membrane. EMBO J. 2007;26:4053-65 pubmed..We propose that Exo70 mediates the assembly of the exocyst complex at the plasma membrane, which is a crucial step in the tethering of post-Golgi secretory vesicles for exocytosis. ..
- Li L, Xu K, Bonini N. Suppression of polyglutamine toxicity by the yeast Sup35 prion domain in Drosophila. J Biol Chem. 2007;282:37694-701 pubmed..These results suggest a trans effect of prion domains on pathogenic poly(Q) disease proteins in a multicellular environment and raise the possibility that Drosophila may allow studies of prion mechanisms. ..
- Singh L, Kruger W. Functional rescue of mutant human cystathionine beta-synthase by manipulation of Hsp26 and Hsp70 levels in Saccharomyces cerevisiae. J Biol Chem. 2009;284:4238-45 pubmed publisher..Based on these results we propose a novel model in which the ratio of Hsp70 and Hsp26 determines whether misfolded proteins will either be refolded or degraded...
- Desantis M, Leung E, Sweeny E, Jackrel M, Cushman Nick M, Neuhaus Follini A, et al. Operational plasticity enables hsp104 to disaggregate diverse amyloid and nonamyloid clients. Cell. 2012;151:778-793 pubmed publisher..Thus, operational plasticity enables Hsp104 to robustly dissolve amyloid and nonamyloid clients, which impose distinct mechanical demands. ..
- Shao D, Creasy C, Bergman L. A cysteine residue in helixII of the bHLH domain is essential for homodimerization of the yeast transcription factor Pho4p. Nucleic Acids Res. 1998;26:710-4 pubmed..Experiments support the role of an intermolecular disulfide bond in stabilization of homodimerization, which is critical for DNA binding. ..
- Huang E, Zhang J, Miska E, Guenther M, Kouzarides T, Lazar M. Nuclear receptor corepressors partner with class II histone deacetylases in a Sin3-independent repression pathway. Genes Dev. 2000;14:45-54 pubmed..This is the first example of a single corepressor utilizing distinct domains to engage multiple HDAC complexes. The alternative HDAC complexes may mediate specific repression pathways in normal as well as leukemic cells. ..
- Lo W, Trievel R, Rojas J, Duggan L, Hsu J, Allis C, et al. Phosphorylation of serine 10 in histone H3 is functionally linked in vitro and in vivo to Gcn5-mediated acetylation at lysine 14. Mol Cell. 2000;5:917-26 pubmed..These observations suggest that transcriptional regulation occurs by multiple mechanistically linked covalent modifications of histones. ..
- Gubitz A, Mourelatos Z, Abel L, Rappsilber J, Mann M, Dreyfuss G. Gemin5, a novel WD repeat protein component of the SMN complex that binds Sm proteins. J Biol Chem. 2002;277:5631-6 pubmed..The presence of 13 WD repeat domains in the amino-terminal half of Gemin5 and a coiled-coil motif near its carboxyl terminus indicate that it may form a large heteromeric complex and engage in multiple interactions. ..
- Zhao F, McCarrick Walmsley R, Akerblad P, Sigvardsson M, Kadesch T. Inhibition of p300/CBP by early B-cell factor. Mol Cell Biol. 2003;23:3837-46 pubmed..Our data reveal an intriguing inhibitory property of EBF-a property shared only by E1A, Twist, Pu.1, and the Hox family of homeodomain proteins-and suggest that E47 and EBF play distinct roles during lambda5 promoter activation. ..
- Germann M, Swain E, Bergman L, Nickels J. Characterizing the sphingolipid signaling pathway that remediates defects associated with loss of the yeast amphiphysin-like orthologs, Rvs161p and Rvs167p. J Biol Chem. 2005;280:4270-8 pubmed..Based on our results, we suggest that sphingolipid-dependent suppression of rvs defects may not work entirely through regulating changes in actin organization. ..
- He B, Xi F, Zhang J, TerBush D, Zhang X, Guo W. Exo70p mediates the secretion of specific exocytic vesicles at early stages of the cell cycle for polarized cell growth. J Cell Biol. 2007;176:771-7 pubmed..Our study suggests that Exo70p functions primarily at early stages of the cell cycle in Bgl2p vesicle secretion, which is critical for polarized cell growth. ..
- Tong F, Billheimer J, Shechtman C, Liu Y, Crooke R, Graham M, et al. Decreased expression of ARV1 results in cholesterol retention in the endoplasmic reticulum and abnormal bile acid metabolism. J Biol Chem. 2010;285:33632-41 pubmed publisher..These studies indicate a critical role of mammalian Arv1p in sterol movement from the ER and in the ensuing regulation of hepatic cholesterol and bile acid metabolism. ..
- Reardon B, Winters R, Gordon D, Winter E. A peptide motif that recognizes A.T tracts in DNA. Proc Natl Acad Sci U S A. 1993;90:11327-31 pubmed..Amino acid similarities with other DNA binding proteins suggest that the DAT1 protein pentad represents a specialized example of a widespread motif used by proteins to recognize A.T base pairs. ..
- Gruda M, Zabolotny J, Xiao J, Davidson I, Alwine J. Transcriptional activation by simian virus 40 large T antigen: interactions with multiple components of the transcription complex. Mol Cell Biol. 1993;13:961-9 pubmed..TEF-1 bound neither region T1 nor a region between amino acids 168 and 373 (region T2); however, it bound efficiently to the combined region (T5) containing amino acids 5 to 383.(ABSTRACT TRUNCATED AT 250 WORDS) ..
- Sakamuro D, Elliott K, Wechsler Reya R, Prendergast G. BIN1 is a novel MYC-interacting protein with features of a tumour suppressor. Nat Genet. 1996;14:69-77 pubmed..Deficits were functionally significant because ectopic expression of BIN1 inhibited the growth of tumour cells lacking endogenous message. We conclude that BIN1 is an MYC-interacting protein with features of a tumour suppressor. ..
- Wang L, Mizzen C, Ying C, Candau R, Barlev N, Brownell J, et al. Histone acetyltransferase activity is conserved between yeast and human GCN5 and is required for complementation of growth and transcriptional activation. Mol Cell Biol. 1997;17:519-27 pubmed..These findings directly link enzymatic histone acetylation and transcriptional activation and show evolutionary conservation of this potentially crucial pathway in gene regulation. ..
- Luo J, Vallen E, Dravis C, Tcheperegine S, Drees B, Bi E. Identification and functional analysis of the essential and regulatory light chains of the only type II myosin Myo1p in Saccharomyces cerevisiae. J Cell Biol. 2004;165:843-55 pubmed..We also identify Mlc2p as the regulatory light chain for Myo1p and demonstrate its role in Myo1p ring disassembly, a function likely conserved among eukaryotes. ..
- Krasley E, Cooper K, Mallory M, Dunbrack R, Strich R. Regulation of the oxidative stress response through Slt2p-dependent destruction of cyclin C in Saccharomyces cerevisiae. Genetics. 2006;172:1477-86 pubmed..These findings demonstrate a new role for the Slt2p MAP kinase cascade in protecting the cell from programmed cell death through cyclin C destruction. ..
- Lee J, Mogen J, Chavez A, Johnson F. Sgs1 RecQ helicase inhibits survival of Saccharomyces cerevisiae cells lacking telomerase and homologous recombination. J Biol Chem. 2008;283:29847-58 pubmed publisher..This provides a novel mechanism by which RecQ helicases may help maintain genome integrity and thus prevent age-related diseases and cancer. ..
- Song D, Lee F. Mouse knock-out of IOP1 protein reveals its essential role in mammalian cytosolic iron-sulfur protein biogenesis. J Biol Chem. 2011;286:15797-805 pubmed publisher..The findings demonstrate an essential role for IOP1 in this pathway. ..
- Kurischko C, Kuravi V, Herbert C, Luca F. Nucleocytoplasmic shuttling of Ssd1 defines the destiny of its bound mRNAs. Mol Microbiol. 2011;81:831-49 pubmed publisher..Collectively these data support the model that Ssd1 functions analogously to hnRNPs, which bind mRNA co-transcriptionally, are exported to the cytoplasm and target mRNAs to sites of localized translation and P-bodies. ..
- Deakyne J, Huang F, Negri J, Tolliday N, Cocklin S, Mazin A. Analysis of the activities of RAD54, a SWI2/SNF2 protein, using a specific small-molecule inhibitor. J Biol Chem. 2013;288:31567-80 pubmed publisher..Thus, the use of SN enabled us to identify important differences in the effect of the RAD54 ATPase and DNA translocation on two major activities of RAD54, BM of Holliday junctions and stimulation of DNA pairing...
- Katz D, Lazar M. Dominant negative activity of an endogenous thyroid hormone receptor variant (alpha 2) is due to competition for binding sites on target genes. J Biol Chem. 1993;268:20904-10 pubmed..Furthermore, a TR alpha 2 mutant which binds more avidly to TREs was a more effective inhibitor of T3 action than wild type TR alpha 2. Together these data indicate that TR alpha 2 inhibits T3 action by competing for binding to TREs. ..
- Creasy C, Madden S, Bergman L. Molecular analysis of the PHO81 gene of Saccharomyces cerevisiae. Nucleic Acids Res. 1993;21:1975-82 pubmed..This suggests that PHO81 may function by interacting with PHO80 or that these molecules compete for the same target. ..
- Timblin B, Tatchell K, Bergman L. Deletion of the gene encoding the cyclin-dependent protein kinase Pho85 alters glycogen metabolism in Saccharomyces cerevisiae. Genetics. 1996;143:57-66 pubmed
- Knight S, Sepuri N, Pain D, Dancis A. Mt-Hsp70 homolog, Ssc2p, required for maturation of yeast frataxin and mitochondrial iron homeostasis. J Biol Chem. 1998;273:18389-93 pubmed..This function for a mitochondrial Hsp70 chaperone is likely to be conserved, implying that a human homolog of Ssc2p may be involved in iron homeostasis and in neurodegenerative disease. ..
- Cooper K, Mallory M, Strich R. Oxidative stress-induced destruction of the yeast C-type cyclin Ume3p requires phosphatidylinositol-specific phospholipase C and the 26S proteasome. Mol Cell Biol. 1999;19:3338-48 pubmed..Taken together, these findings support a model in which Plc1p mediates an oxidative-stress signal from the plasma membrane that triggers Ume3p destruction through a Doa4p-dependent mechanism. ..
- Tomayko M, Punt J, Bolcavage J, Levy S, Allman D, Cancro M. Expression of the Bcl-2 family member A1 is developmentally regulated in T cells. Int Immunol. 1999;11:1753-61 pubmed..Together, these data show that A1 expression is developmentally regulated in T lymphocytes and is responsive to TCR signaling, suggesting that A1 may play a role in maintaining the viability of DP thymocytes. ..
- Belotserkovskaya R, Sterner D, Deng M, Sayre M, Lieberman P, Berger S. Inhibition of TATA-binding protein function by SAGA subunits Spt3 and Spt8 at Gcn4-activated promoters. Mol Cell Biol. 2000;20:634-47 pubmed..These results suggest that the composition of SAGA may be dynamic in vivo and may be regulated through dissociable inhibitory subunits. ..
- Hartman T, Stead K, Koshland D, Guacci V. Pds5p is an essential chromosomal protein required for both sister chromatid cohesion and condensation in Saccharomyces cerevisiae. J Cell Biol. 2000;151:613-26 pubmed..D. Koshland, and A. Strunnikov. 1997. Cell. 91:47-57). Therefore, the link between cohesion and condensation is a general property of yeast chromosomes. ..
- Lindgren A, Bungard D, Pierce M, Xie J, Vershon A, Winter E. The pachytene checkpoint in Saccharomyces cerevisiae requires the Sum1 transcriptional repressor. EMBO J. 2000;19:6489-97 pubmed
- Deng Z, Chen C, Zerby D, Delecluse H, Lieberman P. Identification of acidic and aromatic residues in the Zta activation domain essential for Epstein-Barr virus reactivation. J Virol. 2001;75:10334-47 pubmed
- Toby G, Gherraby W, Coleman T, Golemis E. A novel RING finger protein, human enhancer of invasion 10, alters mitotic progression through regulation of cyclin B levels. Mol Cell Biol. 2003;23:2109-22 pubmed..These and other points lead to a model in which HEI10 defines a divergent class of E3 ubiquitin ligase, functioning in progression through G(2)/M. ..
- Teyssier E, Hirokawa G, Tretiakova A, Jameson B, Kaji A, Kaji H. Temperature-sensitive mutation in yeast mitochondrial ribosome recycling factor (RRF). Nucleic Acids Res. 2003;31:4218-26 pubmed..We propose that Rrf1p is essential for mitochondrial protein synthesis and acts as a RRF in mitochondria...
- Knight J, Daly T, Bergman L. Regulation by phosphorylation of Pho81p, a cyclin-dependent kinase inhibitor in Saccharomyces cerevisiae. Curr Genet. 2004;46:10-9 pubmed..These findings support the observation that the mutant fails to inhibit kinase activity in low phosphate. These studies provide insight into the mechanism of regulation of CDK inhibitor activity. ..
- Burd C, Strochlic T, Setty S. Arf-like GTPases: not so Arf-like after all. Trends Cell Biol. 2004;14:687-94 pubmed..Other Arl proteins are localized to the cytosol, nucleus, cytoskeleton and mitochondria, which indicates that Arl proteins have diverse roles that are distinct from the known functions of traditional Arf GTPases. ..
- Schindler K, Winter E. Phosphorylation of Ime2 regulates meiotic progression in Saccharomyces cerevisiae. J Biol Chem. 2006;281:18307-16 pubmed..These data suggest that Ime2p plays a role in controlling the exit from meiosis I and demonstrate that a phospho-modification pathway regulates Ime2p during the different phases of meiotic development. ..
- Huang J, Song D, Flores A, Zhao Q, Mooney S, Shaw L, et al. IOP1, a novel hydrogenase-like protein that modulates hypoxia-inducible factor-1alpha activity. Biochem J. 2007;401:341-52 pubmed..The results collectively provide evidence that IOP1 is a component of the protein network that regulates HIF-1alpha in mammalian cells. ..
- Zhang X, Orlando K, He B, Xi F, Zhang J, Zajac A, et al. Membrane association and functional regulation of Sec3 by phospholipids and Cdc42. J Cell Biol. 2008;180:145-58 pubmed publisher..We propose that the interactions of Sec3 with phospholipids and Cdc42 play important roles in exocytosis and polarized cell growth. ..
- Cooper K, Mallory M, Guacci V, Lowe K, Strich R. Pds1p is required for meiotic recombination and prophase I progression in Saccharomyces cerevisiae. Genetics. 2009;181:65-79 pubmed publisher..This role is meiosis specific as Mcd1p destruction is not altered in vegetative pds1Delta cultures. These results define a previously undescribed role for Pds1p in cohesin maintenance, recombination, and meiotic progression. ..
- Baek K, Knödler A, Lee S, Zhang X, Orlando K, Zhang J, et al. Structure-function study of the N-terminal domain of exocyst subunit Sec3. J Biol Chem. 2010;285:10424-33 pubmed publisher..Through its ability to bind the small GTPase Cdc42 and phospholipids, the PH domain of Sec3 functions as a coincidence detector at the plasma membrane. ..
- Veríssimo A, Sanders J, Daldal F, Sanders C. Engineering a prokaryotic apocytochrome c as an efficient substrate for Saccharomyces cerevisiae cytochrome c heme lyase. Biochem Biophys Res Commun. 2012;424:130-5 pubmed publisher..This finding led us to propose a consensus sequence located at the amino-terminus of apocytochromes c, and critical for substrate recognition and heme ligation by CCHL. ..
- Jackrel M, Desantis M, Martinez B, Castellano L, Stewart R, Caldwell K, et al. Potentiated Hsp104 variants antagonize diverse proteotoxic misfolding events. Cell. 2014;156:170-82 pubmed publisher..Our work establishes that disease-associated aggregates and amyloid are tractable targets and that enhanced disaggregases can restore proteostasis and mitigate neurodegeneration. ..
- Candau R, Moore P, Wang L, Barlev N, Ying C, Rosen C, et al. Identification of human proteins functionally conserved with the yeast putative adaptors ADA2 and GCN5. Mol Cell Biol. 1996;16:593-602 pubmed..These data indicated that the human proteins correspond to functional homologs of the yeast adaptors, suggesting that these cofactors play a key role in transcriptional activation. ..
- Sells M, Barratt J, Caviston J, Ottilie S, Leberer E, Chernoff J. Characterization of Pak2p, a pleckstrin homology domain-containing, p21-activated protein kinase from fission yeast. J Biol Chem. 1998;273:18490-8 pubmed..pombe encodes at least two pak genes with distinct functions and suggest that the membrane localization of Pak2p, directed by its interactions with membrane lipids and Cdc42p, is critical to its biological activity. ..
- Bulman A, Hubl S, Nelson H. The DNA-binding domain of yeast heat shock transcription factor independently regulates both the N- and C-terminal activation domains. J Biol Chem. 2001;276:40254-62 pubmed..In addition, the DNA-binding domain may mediate key interactions with ancillary factors and/or other intramolecular regulatory regions in order to modulate the complex regulation of HSF's transcriptional activity. ..
- Kim V, Kataoka N, Dreyfuss G. Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex. Science. 2001;293:1832-6 pubmed..The splicing-dependent binding of hUpf3 to mRNAs before export, as part of the complex that assembles near exon-exon junctions, allows it to serve as a link between splicing and NMD in the cytoplasm. ..
- Cooper K, Strich R. Saccharomyces cerevisiae C-type cyclin Ume3p/Srb11p is required for efficient induction and execution of meiotic development. Eukaryot Cell. 2002;1:66-74 pubmed..In conclusion, these findings indicate that Srb11p is required for both entry into and execution of the meiotic program, thus describing multiple roles for a C-type cyclin in the regulation of a developmental pathway. ..
- Cotteret S, Jaffer Z, Beeser A, Chernoff J. p21-Activated kinase 5 (Pak5) localizes to mitochondria and inhibits apoptosis by phosphorylating BAD. Mol Cell Biol. 2003;23:5526-39 pubmed..These features make Pak5 unique among the Pak family and suggest that it plays an important role in apoptosis through BAD phosphorylation. ..
- Mallory M, Strich R. Ume1p represses meiotic gene transcription in Saccharomyces cerevisiae through interaction with the histone deacetylase Rpd3p. J Biol Chem. 2003;278:44727-34 pubmed..In addition, these results suggest that Rpd3-Ume1p-Sin3p comprises an interdependent complex required for mediating transcriptional repression. ..
- Zhang X, Wang P, Gangar A, Zhang J, Brennwald P, TerBush D, et al. Lethal giant larvae proteins interact with the exocyst complex and are involved in polarized exocytosis. J Cell Biol. 2005;170:273-83 pubmed..We propose that, although Lgl is broadly distributed in the cells, its localized interaction with the exocyst and kinetic activation are important for the establishment and reenforcement of cell polarity. ..
- Westhoff C, Wylie D. Transport characteristics of mammalian Rh and Rh glycoproteins expressed in heterologous systems. Transfus Clin Biol. 2006;13:132-8 pubmed..The relationship of Rh and Rh-glycoproteins to the Amt/Mep ammonium transporters is substantiated with functional transport data and structural modeling. ..
- Vermitsky J, Earhart K, Smith W, Homayouni R, Edlind T, Rogers P. Pdr1 regulates multidrug resistance in Candida glabrata: gene disruption and genome-wide expression studies. Mol Microbiol. 2006;61:704-22 pubmed..C. glabrata provides a promising model for studying the genetic basis of multidrug resistance and its impact on virulence. ..
- Krishnamoorthy T, Chen X, Govin J, Cheung W, Dorsey J, Schindler K, et al. Phosphorylation of histone H4 Ser1 regulates sporulation in yeast and is conserved in fly and mouse spermatogenesis. Genes Dev. 2006;20:2580-92 pubmed..Thus, H4 S1ph may be an evolutionarily ancient histone modification to mark the genome for gamete-associated packaging. ..
- Tan G, Magurno J, Cooper K. Ama1p-activated anaphase-promoting complex regulates the destruction of Cdc20p during meiosis II. Mol Biol Cell. 2011;22:315-26 pubmed publisher..These findings provide a mechanism to temporally down-regulate APC/C(Cdc20) activity as the cells complete meiosis II and form spores. ..
- Yoon H, Zhang Y, Pain J, Lyver E, Lesuisse E, Pain D, et al. Rim2, a pyrimidine nucleotide exchanger, is needed for iron utilization in mitochondria. Biochem J. 2011;440:137-46 pubmed publisher..The results indicate that Rim2 is a pyrimidine exchanger with an additional unique function in promoting mitochondrial iron utilization. ..
- Yoon H, Golla R, Lesuisse E, Pain J, Donald J, Lyver E, et al. Mutation in the Fe-S scaffold protein Isu bypasses frataxin deletion. Biochem J. 2012;441:473-80 pubmed publisher..The mechanism by which the mutant Isu bypasses the absence of frataxin remains to be determined, but could be related to direct effects on Fe-S cluster assembly and/or indirect effects on mitochondrial iron availability. ..
- Silver H, Nissley J, Reed S, Hou Y, Johnson E. A role for SUMO in nucleotide excision repair. DNA Repair (Amst). 2011;10:1243-51 pubmed publisher..Collectively, these results suggest that SIZ-dependent sumoylation may modulate the activity of multiple proteins to promote efficient NER. ..
- Oh Y, Chang K, Orlean P, Wloka C, Deshaies R, Bi E. Mitotic exit kinase Dbf2 directly phosphorylates chitin synthase Chs2 to regulate cytokinesis in budding yeast. Mol Biol Cell. 2012;23:2445-56 pubmed publisher..Our study establishes a mechanism for direct cell cycle control of ECM remodeling during cytokinesis. ..
- Whinston E, Omerza G, Singh A, Tio C, Winter E. Activation of the Smk1 mitogen-activated protein kinase by developmentally regulated autophosphorylation. Mol Cell Biol. 2013;33:688-700 pubmed publisher..They demonstrate that intramolecular autophosphorylation of MAPKs can be regulated and suggest new mechanisms for coupling MAPK outputs to developmental programs. ..
- Wloka C, Vallen E, Thé L, Fang X, Oh Y, Bi E. Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast. J Cell Biol. 2013;200:271-86 pubmed publisher..displayed Myo1-dependent immobility during cytokinesis, suggesting that Myo1 plays a scaffolding role in the assembly of a cytokinesis machine. ..
- Creasy C, Chernoff J. Cloning and characterization of a member of the MST subfamily of Ste20-like kinases. Gene. 1995;167:303-6 pubmed..The identification of MST2 suggests that there may be subfamilies of Ste20-like protein kinases and that MST1 and MST2 may define one of these subfamilies. ..
- Fishel R, Wilson T. MutS homologs in mammalian cells. Curr Opin Genet Dev. 1997;7:105-13 pubmed..The redundant functions of MSH3 and MSH6 explain the greater prevalence of hmsh2 mutations in HNPCC families. ..
- Luca F, Mody M, Kurischko C, Roof D, Giddings T, Winey M. Saccharomyces cerevisiae Mob1p is required for cytokinesis and mitotic exit. Mol Cell Biol. 2001;21:6972-83 pubmed..These results suggest that the MEN functions during the mitosis-to-G(1) transition to control cyclin-CDK inactivation and cytokinesis. ..
- Bieganowski P, Garrison P, Hodawadekar S, Faye G, Barnes L, Brenner C. Adenosine monophosphoramidase activity of Hint and Hnt1 supports function of Kin28, Ccl1, and Tfb3. J Biol Chem. 2002;277:10852-60 pubmed publisher..Functional complementation of all Hnt1 phenotypes was provided by rabbit Hint, which is only 22% identical to yeast Hnt1 but has very similar adenosine monophosphoramidase activity...
- Caviston J, Longtine M, Pringle J, Bi E. The role of Cdc42p GTPase-activating proteins in assembly of the septin ring in yeast. Mol Biol Cell. 2003;14:4051-66 pubmed
- Bothos J, Summers M, Venere M, Scolnick D, Halazonetis T. The Chfr mitotic checkpoint protein functions with Ubc13-Mms2 to form Lys63-linked polyubiquitin chains. Oncogene. 2003;22:7101-7 pubmed..We propose that Chfr may have a role in signaling the presence of mitotic stress induced by microtubule poisons. ..
- Huang K, Geunes Boyer S, Wu S, Dutra A, Favor J, Stambolian D. Organization and annotation of the Xcat critical region: elimination of seven positional candidate genes. Genomics. 2004;83:893-901 pubmed..The expression of Rai2 was quantitatively identical in wild-type and Xcat mutant eyes. These results indicate that the Xcat mutation is within a novel, undiscovered gene. ..
- Li K, Zhao K, Ossareh Nazari B, Da G, Dargemont C, Marmorstein R. Structural basis for interaction between the Ubp3 deubiquitinating enzyme and its Bre5 cofactor. J Biol Chem. 2005;280:29176-85 pubmed..Together, these studies provide novel insights into protein recognition by NTF2-like domains and provide a molecular scaffold for understanding how Ubp3 function is regulated by Bre5 cofactor binding. ..
- Azam M, Lee J, Abraham V, Chanoux R, Schoenly K, Johnson F. Evidence that the S.cerevisiae Sgs1 protein facilitates recombinational repair of telomeres during senescence. Nucleic Acids Res. 2006;34:506-16 pubmed..These observations further define roles for RecQ helicases and related proteins in telomere maintenance. ..
- Bugreev D, Mazina O, Mazin A. Rad54 protein promotes branch migration of Holliday junctions. Nature. 2006;442:590-3 pubmed..13). Novel DNA branch-migration activity is fully consistent with this late homologous recombination function of Rad54 protein. ..
- Shorter J, Lindquist S. Hsp104, Hsp70 and Hsp40 interplay regulates formation, growth and elimination of Sup35 prions. EMBO J. 2008;27:2712-24 pubmed publisher..This activity is reduced when Ssa1, or enhanced when Ssb1, is incorporated into nascent prions. These findings illuminate several facets of the chaperone interplay that underpins [PSI(+)] inheritance. ..
- Soper J, Kehm V, Burd C, Bankaitis V, Lee V. Aggregation of ?-synuclein in S. cerevisiae is associated with defects in endosomal trafficking and phospholipid biosynthesis. J Mol Neurosci. 2011;43:391-405 pubmed publisher..These data suggest new pathogenic mechanisms for ?-synuclein neurotoxicity. ..
- Mason M, Skordalakes E. Insights into Cdc13 dependent telomere length regulation. Aging (Albany NY). 2010;2:731-4 pubmed
- Edwards C, Dang W, Berger S. Histone H4 lysine 20 of Saccharomyces cerevisiae is monomethylated and functions in subtelomeric silencing. Biochemistry. 2011;50:10473-83 pubmed publisher..Our findings reveal the first negative regulatory histone methylation in budding yeast and indicate that H4 K20me1 is evolutionarily conserved from simple to complex eukaryotes. ..
- Wood C, Hung C, Huoh Y, Mousley C, Stefan C, BANKAITIS V, et al. Local control of phosphatidylinositol 4-phosphate signaling in the Golgi apparatus by Vps74 and Sac1 phosphoinositide phosphatase. Mol Biol Cell. 2012;23:2527-36 pubmed publisher..The results establish additive roles of Vps74-mediated and sphingolipid-dependent sorting of Golgi residents. ..
- Tanaka A, Tanizawa H, Sriswasdi S, Iwasaki O, Chatterjee A, Speicher D, et al. Epigenetic regulation of condensin-mediated genome organization during the cell cycle and upon DNA damage through histone H3 lysine 56 acetylation. Mol Cell. 2012;48:532-46 pubmed publisher..ATR (ATM- and Rad3-related) kinase mediates the DNA damage response of condensin-mediated genome organization. Our study describes a function of H3K56 acetylation that neutralizes condensin-mediated genome organization. ..
- Mason M, Wanat J, Harper S, Schultz D, Speicher D, Johnson F, et al. Cdc13 OB2 dimerization required for productive Stn1 binding and efficient telomere maintenance. Structure. 2013;21:109-120 pubmed publisher..We therefore propose that dimerization of the OB2 domain of Cdc13 is required for proper Cdc13, Stn1, Ten1 (CST) assembly and productive telomere capping. ..
- Cushman Nick M, Bonini N, Shorter J. Hsp104 suppresses polyglutamine-induced degeneration post onset in a drosophila MJD/SCA3 model. PLoS Genet. 2013;9:e1003781 pubmed publisher..Thus, we establish the first disaggregase or chaperone treatment administered after the onset of pathogenic protein-induced degeneration that mitigates disease progression. ..
- Liu C, Stonestrom A, Christian T, Yong J, Takase R, Hou Y, et al. Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction. J Biol Chem. 2016;291:10426-36 pubmed publisher..Together, these findings provide new insights into the cytochrome c-tRNA interaction and apoptotic regulation. ..
- Haas S, Thatikunta P, Steplewski A, Johnson E, Khalili K, Amini S. A 39-kD DNA-binding protein from mouse brain stimulates transcription of myelin basic protein gene in oligodendrocytic cells. J Cell Biol. 1995;130:1171-9 pubmed..Thus, Pur alpha, a sequence-specific DNA-binding protein upon binding to MB1 regulatory region may play a significant role in determining the cell type-specific expression of MBP in brain. ..
- Lin E, Orlofsky A, Berger M, Prystowsky M. Characterization of A1, a novel hemopoietic-specific early-response gene with sequence similarity to bcl-2. J Immunol. 1993;151:1979-88 pubmed..These data demonstrate that A1 is a novel early-response gene whose expression is associated with a variety of stimuli and occurs in several hemopoietic cell types. ..
- Siomi M, Fromont M, Rain J, Wan L, Wang F, Legrain P, et al. Functional conservation of the transportin nuclear import pathway in divergent organisms. Mol Cell Biol. 1998;18:4141-8 pubmed..Together, these findings demonstrate that both the M9 signal and the nuclear import machinery utilized by the transportin pathway are conserved in evolution. ..
- Bocker T, Barusevicius A, Snowden T, Rasio D, Guerrette S, Robbins D, et al. hMSH5: a human MutS homologue that forms a novel heterodimer with hMSH4 and is expressed during spermatogenesis. Cancer Res. 1999;59:816-22 pubmed..hMSH5 interacts specifically with hMSH4, confirming the generality of functional heterodimeric interactions in the eukaryotic MutS homologue, which also includes hMSH2-hMSH3 and hMSH2-hMSH6. ..
- Meyn M, Melloy P, Li J, Holloway S. The destruction box of the cyclin Clb2 binds the anaphase-promoting complex/cyclosome subunit Cdc23. Arch Biochem Biophys. 2002;407:189-95 pubmed..Mutations in the D box sequences of Clb2 inhibited interaction with Cdc23 both in vivo and in vitro. Our results provide the first evidence for a direct interaction between an APC/C substrate (Clb2) and an APC/C subunit (Cdc23). ..
- Deng Z, Chen C, Chamberlin M, Lu F, Blobel G, Speicher D, et al. The CBP bromodomain and nucleosome targeting are required for Zta-directed nucleosome acetylation and transcription activation. Mol Cell Biol. 2003;23:2633-44 pubmed..These results suggest that activator- and bromodomain-dependent targeting to oligonucleosomal chromatin is required for stable promoter-bound complex formation and transcription activity. ..