Genomes and Genes
Experts and Doctors on protein binding in Philadelphia, Pennsylvania, United States
Locale: Philadelphia, Pennsylvania, United States
Topic: protein binding
Publications435 found, 100 shown here
- Lee S, Kerff F, Chereau D, Ferron F, Klug A, Dominguez R. Structural basis for the actin-binding function of missing-in-metastasis. Structure. 2007;15:145-55 pubmed..The WH2 of MIM is longer than the prototypical WH2, interacting with all four subdomains of actin. We characterize a similar WH2 at the C terminus of IRSp53 and propose that in these two proteins WH2 performs a scaffolding function. ..
- Laberge M, Yonetani T. Molecular dynamics simulations of hemoglobin A in different states and bound to DPG: effector-linked perturbation of tertiary conformations and HbA concerted dynamics. Biophys J. 2008;94:2737-51 pubmed..The changes are not apparent when comparing the available x-ray crystal structures in the presence and absence of effector, but are striking when comparing the respective dynamic tertiary conformations of the R and T tetramers. ..
- Loftin L, Kienzle M, Yi Y, Collman R. R5X4 HIV-1 coreceptor use in primary target cells: implications for coreceptor entry blocking strategies. J Transl Med. 2011;9 Suppl 1:S3 pubmed publisher..These are important questions to consider given the development of new CCR5 blocking therapies and the prognosis associated with CXCR4 use. ..
- Ji X, Kong J, Liebhaber S. An RNA-protein complex links enhanced nuclear 3' processing with cytoplasmic mRNA stabilization. EMBO J. 2011;30:2622-33 pubmed publisher..The demonstrated linkage of nuclear processing with cytoplasmic stabilization via a common RNA-protein complex establishes a basis for integration of sequential controls critical to robust and sustained expression of a target mRNA. ..
- Pimkina J, Murphy M. Interaction of the ARF tumor suppressor with cytosolic HSP70 contributes to its autophagy function. Cancer Biol Ther. 2011;12:503-9 pubmed publisher..High levels of ARF are characteristic of tumor cells with enhanced MAPK signaling and advanced stage; therefore, these data support the premise that PES may show preferential cytotoxicity to advanced stage cancers. ..
- Doyle G, Wang M, Chou A, Oleynick J, Arnold S, Buono R, et al. In vitro and ex vivo analysis of CHRNA3 and CHRNA5 haplotype expression. PLoS ONE. 2011;6:e23373 pubmed publisher..We conclude that CHRNA5 promoter variants may affect relative risk for NA in some heterozygous individuals. ..
- McGurk L, Bonini N. Protein interacting with C kinase (PICK1) is a suppressor of spinocerebellar ataxia 3-associated neurodegeneration in Drosophila. Hum Mol Genet. 2012;21:76-84 pubmed publisher..These findings indicate that interaction proteins may define a rich source of modifier pathways to target in disease situations. ..
- Rajiv C, Jackson S, Cocklin S, Eisenmesser E, Davis T. The spliceosomal proteins PPIH and PRPF4 exhibit bi-partite binding. Biochem J. 2017;474:3689-3704 pubmed publisher..A discussion of how this bipartite interaction between PPIH and PRPF4 may modulate spliceosomal function is included. ..
- Lutz C, Cooke C, O Connor J, Kobayashi R, Alwine J. The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor. RNA. 1998;4:1493-9 pubmed..This suggests that SF-A and/or the SF-A complex(es) perform an important function in both processing reactions and possibly in last exon definition. ..
- Ramu Y, Klem A, Lu Z. Short variable sequence acquired in evolution enables selective inhibition of various inward-rectifier K+ channels. Biochemistry. 2004;43:10701-9 pubmed..Finally we show that, in the heteromeric GIRK1/4 channels, the GIRK4 and not GIRK1 subunit confers the high affinity for TPN. ..
- Wang G, Covarrubias M. Voltage-dependent gating rearrangements in the intracellular T1-T1 interface of a K+ channel. J Gen Physiol. 2006;127:391-400 pubmed..Oxidative stress in excitable tissues may perturb this interface to modulate Kv4 channel function. ..
- Xu M, Hu T, Smith M, Poethig R. Epigenetic Regulation of Vegetative Phase Change in Arabidopsis. Plant Cell. 2016;28:28-41 pubmed publisher..These results suggest a molecular mechanism for the initiation and maintenance of vegetative phase change in plants. ..
- Schmaier A, Kuo A, Lundberg D, Murray S, Cines D. The expression of high molecular weight kininogen on human umbilical vein endothelial cells. J Biol Chem. 1988;263:16327-33 pubmed..By synthesizing and localizing HMWK to the cell surface, endothelial cells may contribute to the activation of plasma's contact serine zymogens and regulation of tissue cysteine proteases. ..
- Estojak J, Brent R, Golemis E. Correlation of two-hybrid affinity data with in vitro measurements. Mol Cell Biol. 1995;15:5820-9 pubmed..Finally, some interactions were subject to directionality, and their apparent strength depended on the reporter used. Taken together, our results provide a cautionary framework for interpreting affinities from two-hybrid experiments. ..
- Yu F, White S, Zhao Q, Lee F. HIF-1alpha binding to VHL is regulated by stimulus-sensitive proline hydroxylation. Proc Natl Acad Sci U S A. 2001;98:9630-5 pubmed..The data furthermore show that this proline hydroxylation is the primary regulator of VHL binding. ..
- Bulman A, Hubl S, Nelson H. The DNA-binding domain of yeast heat shock transcription factor independently regulates both the N- and C-terminal activation domains. J Biol Chem. 2001;276:40254-62 pubmed..In addition, the DNA-binding domain may mediate key interactions with ancillary factors and/or other intramolecular regulatory regions in order to modulate the complex regulation of HSF's transcriptional activity. ..
- Hakimi M, Bochar D, Schmiesing J, Dong Y, Barak O, Speicher D, et al. A chromatin remodelling complex that loads cohesin onto human chromosomes. Nature. 2002;418:994-8 pubmed..Finally, we present evidence pointing to a role for the ATPase activity of SNF2h in the loading of hRAD21 on chromatin. ..
- Lipman R, Chen J, Evilia C, Vitseva O, Hou Y. Association of an aminoacyl-tRNA synthetase with a putative metabolic protein in archaea. Biochemistry. 2003;42:7487-96 pubmed..The association of Mj1338 with ProRS, and with other components of the protein synthesis machinery, thus suggests the possibility of a closer link between metabolism and decoding in archaea than in eukarya or bacteria...
- Tu Z, Lee F. Subdomain VIII is a specificity-determining region in MEKK1. J Biol Chem. 2003;278:48498-505 pubmed..Collectively these results provide evidence that subdomain VIII of MEKK1 is involved not only in binding to, but also in discrimination of, protein substrates. ..
- Xu Y, Lu Z. Characterization of inward-rectifier K+ channel inhibition by antiarrhythmic piperazine. Biochemistry. 2004;43:15577-83 pubmed..We also examine the channel selectivity of piperazine and its molecular determinants. ..
- Jin S, Zhuo Y, Guo W, Field J. p21-activated Kinase 1 (Pak1)-dependent phosphorylation of Raf-1 regulates its mitochondrial localization, phosphorylation of BAD, and Bcl-2 association. J Biol Chem. 2005;280:24698-705 pubmed..These signals are specific for Pak1, because Src-activated Raf-1 only stimulates the MAP kinase cascade. Thus, our data identify the molecular connections of a Pak1-Raf-1-BAD pathway that is involved in cell survival signaling. ..
- Macaluso M, Montanari M, Marshall C, Gambone A, Tosi G, Giordano A, et al. Cytoplasmic and nuclear interaction between Rb family proteins and PAI-2: a physiological crosstalk in human corneal and conjunctival epithelial cells. Cell Death Differ. 2006;13:1515-22 pubmed
- Acharya P, Tran T, Polli J, Ayrton A, Ellens H, Bentz J. P-Glycoprotein (P-gp) expressed in a confluent monolayer of hMDR1-MDCKII cells has more than one efflux pathway with cooperative binding sites. Biochemistry. 2006;45:15505-19 pubmed..e., the inhibitor became effective. These data and their analysis show that the binding of these substrates must be cooperative, either positive or negative. ..
- Huang P, Xu W, Yoon S, Chen C, Chong P, Unterwald E, et al. Agonist treatment did not affect association of mu opioid receptors with lipid rafts and cholesterol reduction had opposite effects on the receptor-mediated signaling in rat brain and CHO cells. Brain Res. 2007;1184:46-56 pubmed..However, MCD treatment had opposite impacts on MOR signaling in the two tissues: it attenuated MOR-mediated [(35)S]GTPgammaS binding in CPu but enhanced it in CHO-HA-rMOR. ..
- Li C, Andrake M, Dunbrack R, Enders G. A bifunctional regulatory element in human somatic Wee1 mediates cyclin A/Cdk2 binding and Crm1-dependent nuclear export. Mol Cell Biol. 2010;30:116-30 pubmed publisher..These studies identify a novel bifunctional regulatory element in Wee1 that mediates cyclin A/Cdk2 association and nuclear export. ..
- Pyrpassopoulos S, Shuman H, Ostap E. Single-molecule adhesion forces and attachment lifetimes of myosin-I phosphoinositide interactions. Biophys J. 2010;99:3916-22 pubmed publisher..However, attachment durations are short-lived, suggesting that phosphoinositides alone do not provide the mechanical stability required to anchor myo1c to membranes during multiple ATPase cycles. ..
- Xiao Y, Isaacs S. Enzyme-linked immunosorbent assay (ELISA) and blocking with bovine serum albumin (BSA)--not all BSAs are alike. J Immunol Methods. 2012;384:148-51 pubmed publisher..This work draws attention to the fact that not all BSA preparations are alike. It also highlights the need to perform critical controls to ensure that ELISA reactants do not inappropriately bind to the blocking agent. ..
- Lu J, Jha H, Verma S, Sun Z, Banerjee S, Dzeng R, et al. Kaposi's sarcoma-associated herpesvirus-encoded LANA contributes to viral latent replication by activating phosphorylation of survivin. J Virol. 2014;88:4204-17 pubmed publisher..This leads to an increase in viral copy number in KSHV-infected B cells. These studies support a role for LANA in regulating KSHV replication through posttranslation modification in KSHV-infected B cells. ..
- Rao J, Madasu Y, Dominguez R. Mechanism of actin filament pointed-end capping by tropomodulin. Science. 2014;345:463-7 pubmed publisher..We found that Tmod achieves high-affinity binding through several discrete low-affinity interactions, which suggests a mechanism for controlled subunit exchange at the pointed end...
- Gallia G, Safak M, Khalili K. Interaction of the single-stranded DNA-binding protein Puralpha with the human polyomavirus JC virus early protein T-antigen. J Biol Chem. 1998;273:32662-9 pubmed..The implication of these findings with respect to the cellular role of Puralpha is discussed...
- Xu W, Li J, Chen C, Huang P, Weinstein H, Javitch J, et al. Comparison of the amino acid residues in the sixth transmembrane domains accessible in the binding-site crevices of mu, delta, and kappa opioid receptors. Biochemistry. 2001;40:8018-29 pubmed
- Hartman H, Hu X, Tyler K, Dalal C, Lazar M. Mechanisms regulating adipocyte expression of resistin. J Biol Chem. 2002;277:19754-61 pubmed..TZD reduces resistin gene expression at least in part by reducing histone acetylation associated with the binding of C/EBPalpha in mature adipocytes. ..
- Yang J, Chennathukuzhi V, Miki K, O Brien D, Hecht N. Mouse testis brain RNA-binding protein/translin selectively binds to the messenger RNA of the fibrous sheath protein glyceraldehyde 3-phosphate dehydrogenase-S and suppresses its translation in vitro. Biol Reprod. 2003;68:853-9 pubmed..These data suggest that TB-RBP is involved in the posttranscriptional regulation of Gapds gene expression during spermiogenesis. Moreover, the Gapds mRNA is the first mRNA shown to have a functional TB-RBP binding site in its 5' UTR. ..
- Awasthi S, Alwine J. Association of polyadenylation cleavage factor I with U1 snRNP. RNA. 2003;9:1400-9 pubmed..Because mAb 2.73 has been used in a number of previous studies related to the U1 snRNP and the U1 70K protein, the precipitation of CF Im must be considered in evaluating past and future data based on the use of mAb 2.73. ..
- Ishino T, Pasut G, Scibek J, Chaiken I. Kinetic interaction analysis of human interleukin 5 receptor alpha mutants reveals a unique binding topology and charge distribution for cytokine recognition. J Biol Chem. 2004;279:9547-56 pubmed..These results suggest that the IL5 x IL5Ralpha system adopts a unique binding topology, in which the cytokine is recognized by a D2D3 tandem domain combined with a D1 domain, to form an extended cytokine recognition interface. ..
- Xu Y, Liu Y, Yu Q. Angiopoietin-3 is tethered on the cell surface via heparan sulfate proteoglycans. J Biol Chem. 2004;279:41179-88 pubmed
- Payne A, Hanakawa Y, Amagai M, Stanley J. Desmosomes and disease: pemphigus and bullous impetigo. Curr Opin Cell Biol. 2004;16:536-43 pubmed..These studies offer new insight into the potential mechanisms of acantholysis in pemphigus and staphylococcal-associated blistering disease, with implications for the role of desmogleins in desmosomal structure and function...
- Shcherbik N, Kee Y, Lyon N, Huibregtse J, Haines D. A single PXY motif located within the carboxyl terminus of Spt23p and Mga2p mediates a physical and functional interaction with ubiquitin ligase Rsp5p. J Biol Chem. 2004;279:53892-8 pubmed..They also suggest that the LPKY motif of Mga2p is required for Rsp5p binding and ubiquitination, and Rsp5p regulates Mga2p function by a mechanism that is independent of providing the partial degradation signal. ..
- Nasrallah I, Minarcik J, Golden J. A polyalanine tract expansion in Arx forms intranuclear inclusions and results in increased cell death. J Cell Biol. 2004;167:411-6 pubmed..Our data suggest expansions in one of the ARX polyA tracts results in nuclear protein aggregation and an increase in cell death; likely underlying the pathogenesis of the associated infantile spasms and mental retardation. ..
- He Z, Russell J. Effect of zeta-globin substitution on the O2-transport properties of Hb S in vitro and in vivo. Biochem Biophys Res Commun. 2004;325:1376-82 pubmed..The potential structural bases for the antisickling properties of Hb zeta(2)beta(2)(S) are discussed in the context of these new observations. ..
- Saiz L, Klein M. The transmembrane domain of the acetylcholine receptor: insights from simulations on synthetic peptide models. Biophys J. 2005;88:959-70 pubmed..The arginine amino acids form intermolecular salt-bridges with the C-terminus, which contribute as well to the bundle stabilization. ..
- Li K, Zhao K, Ossareh Nazari B, Da G, Dargemont C, Marmorstein R. Structural basis for interaction between the Ubp3 deubiquitinating enzyme and its Bre5 cofactor. J Biol Chem. 2005;280:29176-85 pubmed..Together, these studies provide novel insights into protein recognition by NTF2-like domains and provide a molecular scaffold for understanding how Ubp3 function is regulated by Bre5 cofactor binding. ..
- Wan L, Battle D, Yong J, Gubitz A, Kolb S, Wang J, et al. The survival of motor neurons protein determines the capacity for snRNP assembly: biochemical deficiency in spinal muscular atrophy. Mol Cell Biol. 2005;25:5543-51 pubmed..Thus, SMN determines the capacity for snRNP biogenesis, and our findings provide evidence for a measurable deficiency in a biochemical activity in cells from patients with spinal muscular atrophy. ..
- Hollingworth S, Zeiger U, Baylor S. Comparison of the myoplasmic calcium transient elicited by an action potential in intact fibres of mdx and normal mice. J Physiol. 2008;586:5063-75 pubmed publisher..The changes in flux are probably due to a change in the gating of the SR Ca2+-release channels and/or in their single channel flux. The link between these changes and the absence of dystrophin remains to be elucidated. ..
- Taylor J, Bock C, Takusagawa F, Markham G. Discovery of novel types of inhibitors of S-adenosylmethionine synthesis by virtual screening. J Med Chem. 2009;52:5967-73 pubmed publisher..These results validate in silico screening as a robust approach to the discovery of inhibitors of this chemotherapeutically relevant enzyme. ..
- Xu H, Dhanasekaran D, Lee C, Reddy E. Regulation of neurite outgrowth by interactions between the scaffolding protein, JNK-associated leucine zipper protein, and neuronal growth-associated protein superior cervical ganglia clone 10. J Biol Chem. 2010;285:3548-53 pubmed publisher..Taken together, our results suggest that JLP negatively regulates NGF-induced neurite outgrowth through a sequestering mechanism that results in an attenuation of NGF-induced SCG10 phosphorylation. ..
- Meng H, Tian L, Zhou J, Li Z, Jiao X, Li W, et al. PACSIN 2 represses cellular migration through direct association with cyclin D1 but not its alternate splice form cyclin D1b. Cell Cycle. 2011;10:73-81 pubmed..The cyclin D1-associated protein, PACSIN 2, regulates cell spreading and migration, which are dependent on cyclin D1 expression. ..
- Tuszynski G, Rothman V, Zheng X, Gutu M, Zhang X, Chang F. G-protein coupled receptor-associated sorting protein 1 (GASP-1), a potential biomarker in breast cancer. Exp Mol Pathol. 2011;91:608-13 pubmed publisher..These studies identify GASP-1 as a potential new serum and tumor biomarker for breast cancer and suggest that GASP-1 may be a novel target for the development of breast cancer therapeutics. ..
- Kortagere S, Lill M, Kerrigan J. Role of computational methods in pharmaceutical sciences. Methods Mol Biol. 2012;929:21-48 pubmed..These in silico methods are complementary and can be easily integrated into the traditional in vitro and in vivo methods to test pharmacological hypothesis. ..
- Pan X, Papasani M, Hao Y, Calamito M, Wei F, Quinn Iii W, et al. YY1 controls Ig? repertoire and B-cell development, and localizes with condensin on the Ig? locus. EMBO J. 2013;32:1168-82 pubmed publisher..Knock-down of a condensin subunit protein or YY1 reduced rearrangement of Ig? V? genes suggesting a direct role for YY1-condensin complexes in Ig? locus structure and rearrangement. ..
- Gumireddy K, Li A, Yan J, Setoyama T, Johannes G, Orom U, et al. Identification of a long non-coding RNA-associated RNP complex regulating metastasis at the translational step. EMBO J. 2013;32:2672-84 pubmed publisher..Translational suppression by treRNA is dependent on the 3'UTR of the E-cadherin mRNA. Taken together, our study indicates a novel mechanism of gene regulation by lncRNAs in cancer progression. ..
- Paszek M, DuFort C, Rossier O, Bainer R, Mouw J, Godula K, et al. The cancer glycocalyx mechanically primes integrin-mediated growth and survival. Nature. 2014;511:319-25 pubmed publisher..Thus, a bulky glycocalyx is a feature of tumour cells that could foster metastasis by mechanically enhancing cell-surface receptor function. ..
- Tutton S, Azzam G, STONG N, Vladimirova O, Wiedmer A, Monteith J, et al. Subtelomeric p53 binding prevents accumulation of DNA damage at human telomeres. EMBO J. 2016;35:193-207 pubmed publisher..We propose that p53-associated chromatin modifications enhance local DNA repair or protection to provide a previously unrecognized tumor suppressor function of p53. ..
- Lieberman P, Ozer J, Gürsel D. Requirement for transcription factor IIA (TFIIA)-TFIID recruitment by an activator depends on promoter structure and template competition. Mol Cell Biol. 1997;17:6624-32 pubmed..These results indicate that D-A complex recruitment is one of at least two activation pathways utilized by Zta and is the essential pathway for a subset of promoters and conditions which limit TFIID binding to the TATA element. ..
- Reed C, Fu Z, Wu J, Xue Y, Harrison R, Chen M, et al. Crystal structure of TNF-alpha mutant R31D with greater affinity for receptor R1 compared with R2. Protein Eng. 1997;10:1101-7 pubmed..Analysis of the structure and function of receptor-discriminating mutants of TNF will help understand the biological role of TNF and facilitate its use as an antitumor agent. ..
- Lavulo L, Sossong T, Brigham Burke M, Doyle M, Cox J, Christianson D, et al. Subunit-subunit interactions in trimeric arginase. Generation of active monomers by mutation of a single amino acid. J Biol Chem. 2001;276:14242-8 pubmed..At the high protein concentrations utilized in the crystallizations, this variant exists as a trimer, but weakened salt link interactions are observed for Lys-308. ..
- Slater S, Seiz J, Stagliano B, Cook A, Milano S, Ho C, et al. Low- and high-affinity phorbol ester and diglyceride interactions with protein kinase C: 1-O-alkyl-2-acyl-sn-glycerol enhances phorbol ester- and diacylglycerol-induced activity but alone does not induce activity. Biochemistry. 2001;40:6085-92 pubmed..The results reveal HAG to be a member of a new class of "nonactivating" compounds that modulate PKC activity by interacting with the low-affinity phorbol ester binding site...
- Zhou Y, Wang J, Wen H, Kucherovsky O, Levitan I. Modulation of Drosophila slowpoke calcium-dependent potassium channel activity by bound protein kinase a catalytic subunit. J Neurosci. 2002;22:3855-63 pubmed..These results are consistent with the hypothesis that both binding of active PKAc to dSlo and phosphorylation of dSlo or some other protein are necessary for channel modulation. ..
- Sax J, El Deiry W. Identification and characterization of the cytoplasmic protein TRAF4 as a p53-regulated proapoptotic gene. J Biol Chem. 2003;278:36435-44 pubmed..These data suggest a correlation that the orphan adaptor protein TRAF4 may play a role in p53-mediated proapoptotic signaling in the response to cellular stress. ..
- Pixley R, Lin Y, Isordia Salas I, Colman R. Fine mapping of the sequences in domain 5 of high molecular weight kininogen (HK) interacting with heparin and zinc. J Thromb Haemost. 2003;1:1791-8 pubmed..Further, a group of histidine residues in sequence range of H475-H485 contributes to Zn++-dependent binding of heparin to HK-D5. ..
- Zajac A, Sun X, Zhang J, Guo W. Cyclical regulation of the exocyst and cell polarity determinants for polarized cell growth. Mol Biol Cell. 2005;16:1500-12 pubmed..We propose that a cyclical regulatory network contributes to the establishment and maintenance of polarized cell growth in yeast. ..
- Battle D, Lau C, Wan L, Deng H, Lotti F, Dreyfuss G. The Gemin5 protein of the SMN complex identifies snRNAs. Mol Cell. 2006;23:273-9 pubmed..Gemin5 therefore functions as the factor that allows the SMN complex to distinguish snRNAs from other cellular RNAs for snRNP biogenesis. ..
- Wei F, Scholer H, Atchison M. Sumoylation of Oct4 enhances its stability, DNA binding, and transactivation. J Biol Chem. 2007;282:21551-60 pubmed..Therefore, sumoylation of Oct4 results in increased stability, DNA binding, and transactivation and provides an important mechanism to regulate Oct4 activity. ..
- Mellert H, Sykes S, Murphy M, McMahon S. The ARF/oncogene pathway activates p53 acetylation within the DNA binding domain. Cell Cycle. 2007;6:1304-6 pubmed..We demonstrate here that both the DNA damage response pathway and the p19ARF/oncogene stress pathway induce the acetylation of p53 at lysine 120. ..
- Gimenez M, Dilks K, Pohlschröder M. Haloferax volcanii twin-arginine translocation substates include secreted soluble, C-terminally anchored and lipoproteins. Mol Microbiol. 2007;66:1597-606 pubmed..Together, this work not only represents the first experimental demonstration of a lipoprotein Tat substrate, but also indicates the presence of an unidentified lipoprotein biogenesis pathway in archaea. ..
- Ricklin D, Ricklin Lichtsteiner S, Markiewski M, Geisbrecht B, Lambris J. Cutting edge: members of the Staphylococcus aureus extracellular fibrinogen-binding protein family inhibit the interaction of C3d with complement receptor 2. J Immunol. 2008;181:7463-7 pubmed..Our study therefore suggests that S. aureus may disrupt both the innate and adaptive immune responses with a single protein module. ..
- Stengel K, Thangavel C, Solomon D, Angus S, Zheng Y, Knudsen E. Retinoblastoma/p107/p130 pocket proteins: protein dynamics and interactions with target gene promoters. J Biol Chem. 2009;284:19265-71 pubmed publisher..Thus, RB is associated with promoters in a manner similar to p107/p130 and that association is modulated by phosphorylation during cell cycle progression. ..
- Lu F, Wikramasinghe P, Norseen J, Tsai K, Wang P, Showe L, et al. Genome-wide analysis of host-chromosome binding sites for Epstein-Barr Virus Nuclear Antigen 1 (EBNA1). Virol J. 2010;7:262 pubmed publisher..We conclude that EBNA1 can interact with a large number of cellular genes and chromosomal loci in latently infected cells, but that these sites are likely to represent a complex ensemble of direct and indirect EBNA1 binding sites. ..
- Ishino T, Economou N, McFadden K, Zaks Zilberman M, Jost M, Baxter S, et al. A protein engineering approach differentiates the functional importance of carbohydrate moieties of interleukin-5 receptor ?. Biochemistry. 2011;50:7546-56 pubmed publisher..The results suggest that unique carbohydrate groups in multiglycosylated receptors can be utilized asymmetrically for function. ..
- Lu F, Tsai K, Chen H, Wikramasinghe P, Davuluri R, Showe L, et al. Identification of host-chromosome binding sites and candidate gene targets for Kaposi's sarcoma-associated herpesvirus LANA. J Virol. 2012;86:5752-62 pubmed publisher..Our data provide a potential mechanism through which LANA may regulate several host cell pathways by direct binding to gene regulatory elements. ..
- Balaburski G, Leu J, Beeharry N, Hayik S, Andrake M, Zhang G, et al. A modified HSP70 inhibitor shows broad activity as an anticancer agent. Mol Cancer Res. 2013;11:219-29 pubmed publisher..PES-Cl is thus a promising new anticancer compound with several notable mechanisms of action. ..
- Fredericks W, McGarvey T, Wang H, Zheng Y, Fredericks N, Yin H, et al. The TERE1 protein interacts with mitochondrial TBL2: regulation of trans-membrane potential, ROS/RNS and SXR target genes. J Cell Biochem. 2013;114:2170-87 pubmed publisher..A TERE1-TBL2 complex likely functions in oxidative/nitrosative stress, lipid metabolism, and SXR signaling pathways in its role as a tumor suppressor. ..
- Bailis W, Yashiro Ohtani Y, Fang T, Hatton R, Weaver C, Artis D, et al. Notch simultaneously orchestrates multiple helper T cell programs independently of cytokine signals. Immunity. 2013;39:148-59 pubmed publisher..Our data provide a paradigm for Notch in hematopoiesis, with Notch simultaneously orchestrating multiple lineage programs, rather than restricting alternate outcomes. ..
- Bradford H, Krishnaswamy S. The Fragment 1 Region of Prothrombin Facilitates the Favored Binding of Fragment 12 to Zymogen and Enforces Zymogen-like Character in the Proteinase. J Biol Chem. 2016;291:11114-23 pubmed publisher..Thus, the presence of K1 in covalent linkage with K2 in the propiece governs the ability of K2 to bind the (pro)catalytic domain in favor of zymogen, thereby enforcing zymogen-like character in the proteinase. ..
- Tsygankov A, Mahajan S, Fincke J, Bolen J. Specific association of tyrosine-phosphorylated c-Cbl with Fyn tyrosine kinase in T cells. J Biol Chem. 1996;271:27130-7 pubmed..The fraction of c-Cbl capable of coprecipitating with Fyn is increased by TcR/CD3 ligation. This increase is likely due to the involvement of Fyn SH2 in the interactions between Fyn and tyrosine-phosphorylated c-Cbl. ..
- Mourelatos Z, Gonatas J, Cinato E, Gonatas N. Cloning and sequence analysis of the human MG160, a fibroblast growth factor and E-selectin binding membrane sialoglycoprotein of the Golgi apparatus. DNA Cell Biol. 1996;15:1121-8 pubmed..These transcripts may represent alternatively spliced mRNAs of the protein or mRNAs encoding closely related proteins of the Golgi apparatus and/or plasma membranes. ..
- Boyd M, Vlatkovic N, Haines D. A novel cellular protein (MTBP) binds to MDM2 and induces a G1 arrest that is suppressed by MDM2. J Biol Chem. 2000;275:31883-90 pubmed..MTBP can induce G(1) arrest, which in turn can be blocked by MDM2. Our results suggest the existence of another growth control pathway that may be regulated, at least in part, by MDM2. ..
- Salicioni A, Xi M, Vanderveer L, Balsara B, Testa J, Dunbrack R, et al. Identification and structural analysis of human RBM8A and RBM8B: two highly conserved RNA-binding motif proteins that interact with OVCA1, a candidate tumor suppressor. Genomics. 2000;69:54-62 pubmed..The conservation of sequence and structure through such an evolutionarily divergent group of organisms suggests an important function for the RBM8 family of proteins. ..
- Parent J, Labrecque P, Driss Rochdi M, Benovic J. Role of the differentially spliced carboxyl terminus in thromboxane A2 receptor trafficking: identification of a distinct motif for tonic internalization. J Biol Chem. 2001;276:7079-85 pubmed..Our data demonstrate the presence of distinct signals for tonic and agonist-induced internalization of TPbeta and represent the first report of a YX(3)phi motif involved in tonic internalization of a cell surface receptor. ..
- Chen C, Graham J, Yan H. Evidence for a replication function of FFA-1, the Xenopus orthologue of Werner syndrome protein. J Cell Biol. 2001;152:985-96 pubmed..However, in the presence of the dominant negative mutant proteins, the stimulation is prevented. These results provide the first direct biochemical evidence of an important role for FFA-1 in DNA replication. ..
- Sterner D, Wang X, Bloom M, Simon G, Berger S. The SANT domain of Ada2 is required for normal acetylation of histones by the yeast SAGA complex. J Biol Chem. 2002;277:8178-86 pubmed..These results suggest that histone and nucleosomal substrate recognition by SAGA involves multiple conserved domains and proteins, beyond those that have been previously identified. ..
- Rassa J, Meyers J, Zhang Y, Kudaravalli R, Ross S. Murine retroviruses activate B cells via interaction with toll-like receptor 4. Proc Natl Acad Sci U S A. 2002;99:2281-6 pubmed..These results identify retroviral proteins that interact with a mammalian toll receptor and show that direct activation by such viruses may initiate in vivo infection pathways...
- Kim Y, Barak L, Caron M, Benovic J. Regulation of arrestin-3 phosphorylation by casein kinase II. J Biol Chem. 2002;277:16837-46 pubmed..Differences between the regulatory roles of arrestin-2 and -3 phosphorylation may contribute to the different cellular functions of these proteins in G protein-coupled receptor signaling and regulation. ..
- Zhu F, King S, Smith E, Levy D, Yuan Y. A Kaposi's sarcoma-associated herpesviral protein inhibits virus-mediated induction of type I interferon by blocking IRF-7 phosphorylation and nuclear accumulation. Proc Natl Acad Sci U S A. 2002;99:5573-8 pubmed..Inhibition of interferon gene expression through a viral protein blocking the activation and nuclear translocation of a crucial transcription factor is a novel mechanism for viral immune evasion. ..
- Nakamura T, Mori T, Tada S, Krajewski W, Rozovskaia T, Wassell R, et al. ALL-1 is a histone methyltransferase that assembles a supercomplex of proteins involved in transcriptional regulation. Mol Cell. 2002;10:1119-28 pubmed..In parallel, H3-K4 is methylated, and histones H3 and H4 are acetylated at this promoter. ..
- Mongiat M, Fu J, Oldershaw R, Greenhalgh R, Gown A, Iozzo R. Perlecan protein core interacts with extracellular matrix protein 1 (ECM1), a glycoprotein involved in bone formation and angiogenesis. J Biol Chem. 2003;278:17491-9 pubmed..Thus, a physiological interaction could also occur in vivo during development and in pathological events, including tissue remodeling and tumor progression. ..
- Argyris E, Pomerantz R. HIV-1 Vif versus APOBEC3G: newly appreciated warriors in the ancient battle between virus and host. Trends Microbiol. 2004;12:145-8 pubmed
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