Experts and Doctors on murine hepatitis virus in Philadelphia, Pennsylvania, United States


Locale: Philadelphia, Pennsylvania, United States
Topic: murine hepatitis virus

Top Publications

  1. Phillips J, Chua M, Seo S, Weiss S. Multiple regions of the murine coronavirus spike glycoprotein influence neurovirulence. J Neurovirol. 2001;7:421-31 pubmed
    ..From these studies, it appears that the interactions of multiple regions of the MHV spike, including the HVR, act in concert to allow for efficient infection of and virulence in the murine central nervous system. ..
  2. Matthews A, Lavi E, Weiss S, Paterson Y. Neither B cells nor T cells are required for CNS demyelination in mice persistently infected with MHV-A59. J Neurovirol. 2002;8:257-64 pubmed
    ..In infected RAG1(-/-) mice, moderately sized, typical demyelinating lesions were identified. Therefore, demyelination can occur in the absence of B and T cells...
  3. Das Sarma J, Scheen E, Seo S, Koval M, Weiss S. Enhanced green fluorescent protein expression may be used to monitor murine coronavirus spread in vitro and in the mouse central nervous system. J Neurovirol. 2002;8:381-91 pubmed
    ..Thus, EGFP-expressing viruses will be useful in the studies of murine coronavirus pathogenesis in mice...
  4. Schwartz T, Fu L, Lavi E. Differential induction of apoptosis in demyelinating and nondemyelinating infection by mouse hepatitis virus. J Neurovirol. 2002;8:392-9 pubmed
    ..The presence of TUNEL staining in oligodendrocytes suggests that apoptosis may play an important role in MHV-induced demyelination...
  5. Navas Martin S, Weiss S. SARS: lessons learned from other coronaviruses. Viral Immunol. 2003;16:461-74 pubmed
    ..As a result of the SARS epidemic, coronaviruses can now be considered as emerging pathogens. Future research on SARS needs to be based on all the knowledge that coronavirologists have generated over more than 30 years of research. ..
  6. Kazi L, Lissenberg A, Watson R, de Groot R, Weiss S. Expression of hemagglutinin esterase protein from recombinant mouse hepatitis virus enhances neurovirulence. J Virol. 2005;79:15064-73 pubmed
  7. MacNamara K, Bender S, Chua M, Watson R, Weiss S. Priming of CD8+ T cells during central nervous system infection with a murine coronavirus is strain dependent. J Virol. 2008;82:6150-60 pubmed publisher
    ..These findings suggest that the failure of RJHM to prime a robust CD8(+) T-cell response during CNS infection is likely due to its failure to replicate in the CLN. ..
  8. Mazaleuskaya L, Veltrop R, Ikpeze N, MARTIN GARCIA J, Navas Martin S. Protective role of Toll-like Receptor 3-induced type I interferon in murine coronavirus infection of macrophages. Viruses. 2012;4:901-23 pubmed publisher
    ..We demonstrate that activation of TLR3 with the synthetic ligand poly I:C mediates antiviral immunity that diminishes (MHV-A59) or suppresses (MHV-JHM, MHV-3) virus production in macrophages. ..
  9. Bender S, Phillips J, Scott E, Weiss S. Murine coronavirus receptors are differentially expressed in the central nervous system and play virus strain-dependent roles in neuronal spread. J Virol. 2010;84:11030-44 pubmed publisher
    ..Therefore, neither CEACAM2 nor PSG16 is likely to be an MHV receptor on neurons, and the mechanism for CEACAM1a-independent neuronal spread of JHM.SD remains unknown. ..

More Information


  1. Bender S, Weiss S. Pathogenesis of murine coronavirus in the central nervous system. J Neuroimmune Pharmacol. 2010;5:336-54 pubmed publisher
  2. Cowley T, Long S, Weiss S. The murine coronavirus nucleocapsid gene is a determinant of virulence. J Virol. 2010;84:1752-63 pubmed publisher
  3. Roth Cross J, Bender S, Weiss S. Murine coronavirus mouse hepatitis virus is recognized by MDA5 and induces type I interferon in brain macrophages/microglia. J Virol. 2008;82:9829-38 pubmed publisher
    ..These findings suggest that secretion of IFN-beta by macrophages and microglia plays a role in protecting the host from MHV infection of the central nervous system. ..
  4. Navas S, Weiss S. Murine coronavirus-induced hepatitis: JHM genetic background eliminates A59 spike-determined hepatotropism. J Virol. 2003;77:4972-8 pubmed
    ..Thus, the JHM genetic background plays a dominant role over the spike in the determination of hepatotropism. ..
  5. Roth Cross J, Martinez Sobrido L, Scott E, Garcia Sastre A, Weiss S. Inhibition of the alpha/beta interferon response by mouse hepatitis virus at multiple levels. J Virol. 2007;81:7189-99 pubmed
    ..These findings show that MHV employs unique strategies to circumvent the IFN-alpha/beta response at multiple steps...
  6. Zhao L, Birdwell L, Wu A, Elliott R, Rose K, Phillips J, et al. Cell-type-specific activation of the oligoadenylate synthetase-RNase L pathway by a murine coronavirus. J Virol. 2013;87:8408-18 pubmed publisher
    ..Thus, RNase L activation during MHV infection is cell type specific and correlates with relatively high levels of expression of OAS genes, which are necessary but not sufficient for induction of an effective RNase L antiviral response. ..
  7. Das Sarma J, Fu L, Hingley S, Lai M, Lavi E. Sequence analysis of the S gene of recombinant MHV-2/A59 coronaviruses reveals three candidate mutations associated with demyelination and hepatitis. J Neurovirol. 2001;7:432-6 pubmed
    ..One or more of the sites of these mutations in the MHV-A59 genome are likely to contribute to demyelination and hepatitis. ..
  8. Zhao L, Jha B, Wu A, Elliott R, Ziebuhr J, Gorbalenya A, et al. Antagonism of the interferon-induced OAS-RNase L pathway by murine coronavirus ns2 protein is required for virus replication and liver pathology. Cell Host Microbe. 2012;11:607-16 pubmed publisher
    ..Thus, RNase L is a critical cellular factor for protection against viral infection of the liver and the resulting hepatitis. ..
  9. Phillips J, Kuo I, Richardson C, Weiss S. A novel full-length isoform of murine pregnancy-specific glycoprotein 16 (psg16) is expressed in the brain but does not mediate murine coronavirus (MHV) entry. J Neurovirol. 2012;18:138-43 pubmed publisher
  10. Weiss S, Leibowitz J. Coronavirus pathogenesis. Adv Virus Res. 2011;81:85-164 pubmed publisher
  11. Roth Cross J, Stokes H, Chang G, Chua M, Thiel V, Weiss S, et al. Organ-specific attenuation of murine hepatitis virus strain A59 by replacement of catalytic residues in the putative viral cyclic phosphodiesterase ns2. J Virol. 2009;83:3743-53 pubmed publisher
    ..This novel phenotype suggests a strategy to investigate the function of the MHV-A59 ns2 protein involving the search for organ-specific proteins or RNAs that react differentially to wild-type and mutant ns2 proteins. ..
  12. Zhao L, Rose K, Elliott R, Van Rooijen N, Weiss S. Cell-type-specific type I interferon antagonism influences organ tropism of murine coronavirus. J Virol. 2011;85:10058-68 pubmed publisher
    ..Our results further support the notion that viral tissue tropism is determined in part by postentry events, including the early type I interferon response. ..
  13. Shindler K, Chatterjee D, Biswas K, Goyal A, Dutt M, Nassrallah M, et al. Macrophage-mediated optic neuritis induced by retrograde axonal transport of spike gene recombinant mouse hepatitis virus. J Neuropathol Exp Neurol. 2011;70:470-80 pubmed publisher
  14. Cowley T, Weiss S. Murine coronavirus neuropathogenesis: determinants of virulence. J Neurovirol. 2010;16:427-34 pubmed publisher
    ..SD. Further data suggest that neither the internal (I) protein nor nonstructural proteins ns4, and ns2 are significant contributors to neurovirulence. ..
  15. Phillips J, Weiss S. Pathogenesis of neurotropic murine coronavirus is multifactorial. Trends Pharmacol Sci. 2011;32:2-7 pubmed publisher
    ..We propose that the extreme neurovirulence of JHM.SD is multifactorial and might include as yet unidentified neuron-specific spread mechanisms. ..
  16. Zhang R, Jha B, Ogden K, Dong B, Zhao L, Elliott R, et al. Homologous 2',5'-phosphodiesterases from disparate RNA viruses antagonize antiviral innate immunity. Proc Natl Acad Sci U S A. 2013;110:13114-9 pubmed publisher
    ..Thus, VP3-CTD is a 2',5'-PDE able to functionally substitute for ns2 in MHV infection. Remarkably, therefore, two disparate RNA viruses encode proteins with homologous 2',5'-PDEs that antagonize activation of innate immunity. ..
  17. Navas Martin S, Hingley S, Weiss S. Murine coronavirus evolution in vivo: functional compensation of a detrimental amino acid substitution in the receptor binding domain of the spike glycoprotein. J Virol. 2005;79:7629-40 pubmed publisher
  18. Shindler K, Kenyon L, Dutt M, Hingley S, Das Sarma J. Experimental optic neuritis induced by a demyelinating strain of mouse hepatitis virus. J Virol. 2008;82:8882-6 pubmed publisher
    ..In contrast, MHV-2, a nondemyelinating MHV strain, does not induce ON. Results reveal a reproducible virus-induced ON model important for the evaluation of novel therapies...
  19. Leparc Goffart I, Hingley S, Chua M, Jiang X, Lavi E, Weiss S. Altered pathogenesis of a mutant of the murine coronavirus MHV-A59 is associated with a Q159L amino acid substitution in the spike protein. Virology. 1997;239:1-10 pubmed publisher
  20. Scott E, Branigan P, Del Vecchio A, Weiss S. Chemokine expression during mouse-hepatitis-virus-induced encephalitis: contributions of the spike and background genes. J Neurovirol. 2008;14:5-16 pubmed publisher
    ..Chemokine mRNA patterns were in general agreement. Thus, chemokine patterns correspond with the cellular infiltrate, and the spike protein influences levels of macrophage, but not T-cell, chemokines...
  21. Matthews A, Weiss S, Paterson Y. Murine hepatitis virus--a model for virus-induced CNS demyelination. J Neurovirol. 2002;8:76-85 pubmed
    ..Here we review this field in the context of the immune response to MHV in the liver and the CNS focusing on studies that have been published in the past 5 years...
  22. Phillips J, Chua M, Rall G, Weiss S. Murine coronavirus spike glycoprotein mediates degree of viral spread, inflammation, and virus-induced immunopathology in the central nervous system. Virology. 2002;301:109-20 pubmed
    ..Despite this robust and viral-specific immune response to S(4)R infection, infection of RAG1-/- mice suggested that immune-mediated pathology also contributes to the high neurovirulence of S(4)R...
  23. Das Sarma J, Iacono K, Gard L, Marek R, Kenyon L, Koval M, et al. Demyelinating and nondemyelinating strains of mouse hepatitis virus differ in their neural cell tropism. J Virol. 2008;82:5519-26 pubmed publisher
  24. Qiu Z, Hingley S, Simmons G, Yu C, Das Sarma J, Bates P, et al. Endosomal proteolysis by cathepsins is necessary for murine coronavirus mouse hepatitis virus type 2 spike-mediated entry. J Virol. 2006;80:5768-76 pubmed