Genomes and Genes
Experts and Doctors on kinetics in Philadelphia, Pennsylvania, United States
Locale: Philadelphia, Pennsylvania, United States
Publications275 found, 100 shown here
- Gunther E, Belka G, Wertheim G, Wang J, Hartman J, Boxer R, et al. A novel doxycycline-inducible system for the transgenic analysis of mammary gland biology. FASEB J. 2002;16:283-92 pubmed..This system offers new opportunities for the transgenic analysis of mammary gland biology in vivo. ..
- Speirs K, Caamano J, Goldschmidt M, Hunter C, Scott P. NF-kappa B2 is required for optimal CD40-induced IL-12 production but dispensable for Th1 cell Differentiation. J Immunol. 2002;168:4406-13 pubmed..major is the result of an IL-12 deficiency, and we provide evidence for a specific impairment in CD40-induced IL-12 production by macrophages lacking this transcription factor. ..
- Heredia V, Penning T. Dissection of the physiological interconversion of 5alpha-DHT and 3alpha-diol by rat 3alpha-HSD via transient kinetics shows that the chemical step is rate-determining: effect of mutating cofactor and substrate-binding pocket residues on catalysis. Biochemistry. 2004;43:12028-37 pubmed..Thus, residues in the cofactor and steroid-binding site can alter the rate-determining step in the NADP(+)-dependent oxidation of 3alpha-diol to make chemistry rate-limiting overall. ..
- Li J, Gran B, Zhang G, Rostami A, Kamoun M. IL-27 subunits and its receptor (WSX-1) mRNAs are markedly up-regulated in inflammatory cells in the CNS during experimental autoimmune encephalomyelitis. J Neurol Sci. 2005;232:3-9 pubmed..Thus, IL-27 produced by infiltrating cells in the CNS may regulate in a paracrine manner the Th1 response in EAE. ..
- Hung S, Zhang W, Pixley R, Jameson B, Huang Y, Colman R, et al. New insights from the structure-function analysis of the catalytic region of human platelet phosphodiesterase 3A: a role for the unique 44-amino acid insert. J Biol Chem. 2006;281:29236-44 pubmed..This change would allow the substrate to bind to Tyr(807) and other amino acids in the insert to interact with residues important for catalysis in the active site cleft. ..
- Casta L, Buguliskis J, Matsumoto Y, Taraschi T. Expression and biochemical characterization of the Plasmodium falciparum DNA repair enzyme, flap endonuclease-1 (PfFEN-1). Mol Biochem Parasitol. 2008;157:1-12 pubmed..Plasmodium FEN-1s have enzymatic activities similar to other species but contain extended C-termini and a more internally located PCNA-binding site. ..
- Taujale S, Zhang H. Impact of interactions between metal oxides to oxidative reactivity of manganese dioxide. Environ Sci Technol. 2012;46:2764-71 pubmed publisher..These mechanisms can also explain the observed adsorption behavior of triclosan by the binary oxide mixtures and single oxides. ..
- Palani S, Sarkar C. Transient noise amplification and gene expression synchronization in a bistable mammalian cell-fate switch. Cell Rep. 2012;1:215-24 pubmed publisher..Finally, we show that this transient noise amplification and gene expression synchronization induced by ligand can directly influence cell survival and differentiation kinetics within the population. ..
- Jerng H, Shahidullah M, Covarrubias M. Inactivation gating of Kv4 potassium channels: molecular interactions involving the inner vestibule of the pore. J Gen Physiol. 1999;113:641-60 pubmed..We propose that components of the pore's internal vestibule control both closing and inactivation in Kv4 K+ channels. ..
- Wali A, Strayer D. Comparative effects of virulent and avirulent poxviruses on cell cycle progression. Exp Mol Pathol. 1999;66:31-8 pubmed..Thus alterations in cell cycle transit during virus infection may reflect active direction in which virus induces changes in cell cycle regulators. Such changes may be important in the differences in virulence between MV and SFV...
- Caloca M, Garcia Bermejo M, Blumberg P, Lewin N, Kremmer E, Mischak H, et al. beta2-chimaerin is a novel target for diacylglycerol: binding properties and changes in subcellular localization mediated by ligand binding to its C1 domain. Proc Natl Acad Sci U S A. 1999;96:11854-9 pubmed..Our results demonstrate that beta2-chimaerin is a high affinity receptor for DAG through binding to its C1 domain and supports the emerging concept that multiple pathways transduce signaling through DAG and the phorbol esters. ..
- Bruening W, Prowse A, Schultz D, Holgado Madruga M, Wong A, Godwin A. Expression of OVCA1, a candidate tumor suppressor, is reduced in tumors and inhibits growth of ovarian cancer cells. Cancer Res. 1999;59:4973-83 pubmed..These results suggest that slight alterations in the level of OVCA1, such as would occur after reduction of chromosome 17p13.13 to hemizygosity, may result in cell cycle deregulation and promote tumorigenesis. ..
- Gudmundsdottir H, Turka L. A closer look at homeostatic proliferation of CD4+ T cells: costimulatory requirements and role in memory formation. J Immunol. 2001;167:3699-707 pubmed..This mechanism of T cell regeneration is possibly active during lymphopenia due to viral infections, such as HIV, transplantation, or cancer therapy, and may explain selected autoimmune diseases. ..
- Baird T, Walsh P. Activated platelets but not endothelial cells participate in the initiation of the consolidation phase of blood coagulation. J Biol Chem. 2002;277:28498-503 pubmed..These results support the concept that activated platelets, but not endothelial cells, expose a procoagulant surface for binding and activating factor XI, thereby initiating the consolidation phase of coagulation. ..
- Li C, Fox C, Master S, Bindokas V, Chodosh L, Thompson C. Bcl-X(L) affects Ca(2+) homeostasis by altering expression of inositol 1,4,5-trisphosphate receptors. Proc Natl Acad Sci U S A. 2002;99:9830-5 pubmed..These results suggest that even under non-apoptotic conditions, expression of Bcl-2-family proteins influences a signaling network that links changes in mitochondrial metabolism to alterations in nuclear gene expression. ..
- Kaji A, Kiel M, Hirokawa G, Muto A, Inokuchi Y, Kaji H. The fourth step of protein synthesis: disassembly of the posttermination complex is catalyzed by elongation factor G and ribosome recycling factor, a near-perfect mimic of tRNA. Cold Spring Harb Symp Quant Biol. 2001;66:515-29 pubmed
- Heredia V, Cooper W, Kruger R, Jin Y, Penning T. Alanine scanning mutagenesis of the testosterone binding site of rat 3 alpha-hydroxysteroid dehydrogenase demonstrates contact residues influence the rate-determining step. Biochemistry. 2004;43:5832-41 pubmed..These findings support the concept of enzyme catalysis in which the correct positioning of reactants is essential; otherwise, k(cat) will be limited by the chemical event. ..
- Spassova M, Avissar M, Furman A, Crumling M, Saunders J, Parsons T. Evidence that rapid vesicle replenishment of the synaptic ribbon mediates recovery from short-term adaptation at the hair cell afferent synapse. J Assoc Res Otolaryngol. 2004;5:376-90 pubmed..This apparent rapid reloading of the synaptic ribbon with vesicles underscores important functional differences between synaptic ribbons in the auditory and visual systems. ..
- Hsu Y, Ferl G, Ng C. GPU-accelerated nonparametric kinetic analysis of DCE-MRI data from glioblastoma patients treated with bevacizumab. Magn Reson Imaging. 2013;31:618-23 pubmed publisher..This finding indicates that the GPU-based method can significantly shorten the computational times required to assess tumor physiology from DCE-MRI data in preclinical and clinical development of antivascular therapies. ..
- Sharma H, Mutharasan R. Half antibody fragments improve biosensor sensitivity without loss of selectivity. Anal Chem. 2013;85:2472-7 pubmed publisher..Examining the half antibody fragments for detection of the pathogen in the presence of the nonpathogenic wild strain showed that the antibody fragments retained their specific antigen binding capability without loss of selectivity. ..
- Freed D, Bessman N, Kiyatkin A, Salazar Cavazos E, Byrne P, Moore J, et al. EGFR Ligands Differentially Stabilize Receptor Dimers to Specify Signaling Kinetics. Cell. 2017;171:683-695.e18 pubmed publisher..Our results also suggest parallels between partial and/or biased agonism in RTKs and G-protein-coupled receptors, as well as new therapeutic opportunities for correcting RTK signaling output. ..
- Dimaculangan D, Chawla A, Boak A, Kagan H, Lazar M. Retinoic acid prevents downregulation of ras recision gene/lysyl oxidase early in adipocyte differentiation. Differentiation. 1994;58:47-52 pubmed..Thus, differential display has been successfully used to identify rrg/lysyl oxidase as an early marker for adipose conversion that is responsive to RA. ..
- Smyth E, Nestor P, Fitzgerald G. Agonist-dependent phosphorylation of an epitope-tagged human prostacyclin receptor. J Biol Chem. 1996;271:33698-704 pubmed..These results indicate that the human prostacyclin receptor may be regulated by agonist-dependent phosphorylation. This appears to be mediated, in part, by activation of PKC but not by PKA. ..
- Jaffe E, Martins J, Li J, Kervinen J, Dunbrack R. The molecular mechanism of lead inhibition of human porphobilinogen synthase. J Biol Chem. 2001;276:1531-7 pubmed..The data support a novel model where a high affinity lead site is a hybrid of the ZnA and ZnB sites. We propose that the lone electron pair of Pb(II) precludes Pb(II) to function in PBGS catalysis. ..
- Gochenauer G, Robinson M. Dibutyryl-cAMP (dbcAMP) up-regulates astrocytic chloride-dependent L-[3H]glutamate transport and expression of both system xc(-) subunits. J Neurochem. 2001;78:276-86 pubmed..Because this activity is thought to be important for the synthesis of glutathione and protection from oxidant injury, understanding the regulation of system xc(-) may provide alternate approaches to limit this form of injury. ..
- Mazurek A, Berardini M, Fishel R. Activation of human MutS homologs by 8-oxo-guanine DNA damage. J Biol Chem. 2002;277:8260-6 pubmed..These results suggest that only hMSH2-hMSH6 is activated by recognition of 8-oxo-G lesions. Our data are consistent with the notion that post-replication MMR only participates in the repair of mismatched 8-oxo-G lesions. ..
- Tao R, Huang K. Reducing blood viscosity with magnetic fields. Phys Rev E Stat Nonlin Soft Matter Phys. 2011;84:011905 pubmed..In addition, such viscosity reduction does not affect the red blood cells' normal function. This technology has much potential for physical therapy. ..
- Torigoe T, O CONNOR R, Santoli D, Reed J. Interleukin-3 regulates the activity of the LYN protein-tyrosine kinase in myeloid-committed leukemic cell lines. Blood. 1992;80:617-24 pubmed..These observations provide the first example of an IL-3--regulable PTK, and strongly suggest that the p53/56-LYN kinase participates in early IL-3--initiated signalling events, at least in some human leukemic cell lines. ..
- Kovachich G, Aronson C, Brunswick D, Frazer A. Quantitative autoradiography of serotonin uptake sites in rat brain using [3H]cyanoimipramine. Brain Res. 1988;454:78-88 pubmed..The properties of binding of [3H]cyanoimipramine make it an ideal ligand for the quantitative autoradiography of serotonin uptake sites...
- Berlin J, Bassani J, Bers D. Intrinsic cytosolic calcium buffering properties of single rat cardiac myocytes. Biophys J. 1994;67:1775-87 pubmed..The present data indicate that a rapid rise of [Ca2+]i from 0.1 to 1 microM during a contraction requires approximately 50 microM Ca2+ to be added to the cytosol. ..
- Görlach M, Burd C, Dreyfuss G. The mRNA poly(A)-binding protein: localization, abundance, and RNA-binding specificity. Exp Cell Res. 1994;211:400-7 pubmed..This excess and the high concentration of the hPABP, which is three orders of magnitude above its Kd for oligo(rA)25, suggest that the hPABP may bind to additional, lower affinity binding sites in vivo. ..
- Margulis A, Goraczniak R, Duda T, Sharma R, Sitaramayya A. Structural and biochemical identity of retinal rod outer segment membrane guanylate cyclase. Biochem Biophys Res Commun. 1993;194:855-61 pubmed..These results suggest that ROS-GC represents a new subtype of the membrane guanylate cyclase family that is structurally and biochemically distinct from the other retinal cyclases. ..
- Grubmeyer C, Segura E, Dorfman R. Active site lysines in orotate phosphoribosyltransferase. J Biol Chem. 1993;268:20299-304 pubmed
- Loll P, Picot D, Ekabo O, Garavito R. Synthesis and use of iodinated nonsteroidal antiinflammatory drug analogs as crystallographic probes of the prostaglandin H2 synthase cyclooxygenase active site. Biochemistry. 1996;35:7330-40 pubmed..No evidence is seen for significant protein conformational differences between the iodoindomethacin and iodosuprofen of flurbiprofen complex structures. ..
- Hisatome I, Morisaki T, Kamma H, Sugama T, Morisaki H, Ohtahara A, et al. Control of AMP deaminase 1 binding to myosin heavy chain. Am J Physiol. 1998;275:C870-81 pubmed..These findings are discussed in the context of the various roles proposed for AMPD in energy production in the myocyte. ..
- Grubmeyer C, Teng H. Mechanism of Salmonella typhimurium histidinol dehydrogenase: kinetic isotope effects and pH profiles. Biochemistry. 1999;38:7355-62 pubmed..Thus, although the chemistry of the first hydride transfer is fast, both partial reactions participate in the overall rate limitation...
- De La Cruz E, Ostap E, Brundage R, Reddy K, Sweeney H, Safer D. Thymosin-beta(4) changes the conformation and dynamics of actin monomers. Biophys J. 2000;78:2516-27 pubmed..The conformational change may reflect the unique ability of Tbeta(4) to sequester actin monomers and inhibit nucleotide exchange. ..
- Ho D, Badellino K, Baglia F, Sun M, Zhao M, Gailani D, et al. The role of high molecular weight kininogen and prothrombin as cofactors in the binding of factor XI A3 domain to the platelet surface. J Biol Chem. 2000;275:25139-45 pubmed..Thus, the interaction of factor XI with platelets is mediated by the amino acid residues Ser(248), Arg(250), Lys(255), Leu(257), Phe(260), and Gln(263) within the Apple 3 domain. ..
- Charkes N. Physiological significance of the rate constants in compartmental analysis. Nucl Med Commun. 2001;22:331-9 pubmed
- Fertala A, Sieron A, Adachi E, Jimenez S. Collagen II containing a Cys substitution for Arg-alpha1-519: abnormal interactions of the mutated molecules with collagen IX. Biochemistry. 2001;40:14422-8 pubmed
- Yi J, Cheng H, Andrake M, Dunbrack R, Roder H, Skalka A. Mapping the epitope of an inhibitory monoclonal antibody to the C-terminal DNA-binding domain of HIV-1 integrase. J Biol Chem. 2002;277:12164-74 pubmed
- Scibek J, Evergren E, Zahn S, Canziani G, Van Ryk D, Chaiken I. Biosensor analysis of dynamics of interleukin 5 receptor subunit beta(c) interaction with IL5:IL5R(alpha) complexes. Anal Biochem. 2002;307:258-65 pubmed
- Cooper K, Strich R. Saccharomyces cerevisiae C-type cyclin Ume3p/Srb11p is required for efficient induction and execution of meiotic development. Eukaryot Cell. 2002;1:66-74 pubmed..In conclusion, these findings indicate that Srb11p is required for both entry into and execution of the meiotic program, thus describing multiple roles for a C-type cyclin in the regulation of a developmental pathway. ..
- Chen Z, Hopper Borge E, Belinsky M, Shchaveleva I, Kotova E, Kruh G. Characterization of the transport properties of human multidrug resistance protein 7 (MRP7, ABCC10). Mol Pharmacol. 2003;63:351-8 pubmed..The determination that MRP7 has the facility for mediating the transport of conjugates such as E(2)17betaG indicates that it is a lipophilic anion transporter involved in phase III (cellular extrusion) of detoxification. ..
- Kashlan O, Cooperman B. Comprehensive model for allosteric regulation of mammalian ribonucleotide reductase: refinements and consequences. Biochemistry. 2003;42:1696-706 pubmed
- Yang D, Moss E. Temporally regulated expression of Lin-28 in diverse tissues of the developing mouse. Gene Expr Patterns. 2003;3:719-26 pubmed
- Ramu Y, Xu Y, Lu Z. Enzymatic activation of voltage-gated potassium channels. Nature. 2006;442:696-9 pubmed..Here we report the apparent activation of voltage-gated K+ (Kv) channels by a sphingomyelinase. ..
- Culyba M, Hwang Y, Minkah N, Bushman F. DNA binding and cleavage by the fowlpox virus resolvase. J Biol Chem. 2009;284:1190-201 pubmed publisher..These findings provide a tractable poxvirus resolvase usable for the development of small molecule inhibitors...
- Chambers J, Hill P, Aaron J, Han Z, Christianson D, Kuzma N, et al. Cryptophane xenon-129 nuclear magnetic resonance biosensors targeting human carbonic anhydrase. J Am Chem Soc. 2009;131:563-9 pubmed publisher..Thus, xenon biosensors may provide a powerful strategy for diagnosing human diseases characterized by the upregulation of specific CA isozymes and other protein biomarkers...
- Covy J, Giasson B. The G2019S pathogenic mutation disrupts sensitivity of leucine-rich repeat kinase 2 to manganese kinase inhibition. J Neurochem. 2010;115:36-46 pubmed publisher..From these findings, a new model is proposed for a possible function of LRRK2 and the consequence of the G2019S LRRK2 pathogenic mutation. ..
- Monaco Shawver L, Schwartz L, Tuluc F, Guo C, Lai J, Gunnam S, et al. Substance P inhibits natural killer cell cytotoxicity through the neurokinin-1 receptor. J Leukoc Biol. 2011;89:113-25 pubmed publisher..Antagonism of this system may present an opportunity to augment NK cell function therapeutically in selected human diseases. ..
- Leggin B, Sheridan S, Eckenrode B. Rehabilitation after surgical management of the thrower's shoulder. Sports Med Arthrosc Rev. 2012;20:49-55 pubmed publisher..The fourth phase, return to throwing, will be covered in the final section. ..
- Morahan P, Pinto A, Stewart D, Murasko D, Brinton M. Varying role of alpha/beta interferon in the antiviral efficacy of synthetic immunomodulators against Semliki Forest virus infection. Antiviral Res. 1991;15:241-54 pubmed..Taken together, these results indicate that induction of interferon alpha/beta does not appear to be the major common mechanism of antiviral activity among these diverse synthetic immunomodulators...
- Karasawa A, Rochester J, Ma X, Lefer A. Protection of endothelial damage and systemic shock by benidipine, a calcium antagonist, in rats subjected to splanchnic ischemia and reperfusion. Circ Shock. 1991;33:135-41 pubmed
- Ozturk D, Dorfman R, Scapin G, Sacchettini J, Grubmeyer C. Locations and functional roles of conserved lysine residues in Salmonella typhimurium orotate phosphoribosyltransferase. Biochemistry. 1995;34:10755-63 pubmed..Mutation of Lys-100 produced a 5-fold decrease in kcat and a 3-fold increase in the KM for PRPP, consistent with its location within the active site, near the pyrophosphate moiety of PRPP.(ABSTRACT TRUNCATED AT 250 WORDS) ..
- Fernandes Alnemri T, Takahashi A, Armstrong R, Krebs J, Fritz L, Tomaselli K, et al. Mch3, a novel human apoptotic cysteine protease highly related to CPP32. Cancer Res. 1995;55:6045-52 pubmed
- Liu F, Hill D, Chernoff J. Direct binding of the proline-rich region of protein tyrosine phosphatase 1B to the Src homology 3 domain of p130(Cas). J Biol Chem. 1996;271:31290-5 pubmed..These results suggest that the proline-rich domain between amino acids 301 and 315 in PTP1B binds Src homology 3-containing proteins and that p130(Cas) may be a physiological target of this phosphatase in cells. ..
- Lu Y, Li Z, Drozdowicz Y, Hortensteiner S, Martinoia E, Rea P. AtMRP2, an Arabidopsis ATP binding cassette transporter able to transport glutathione S-conjugates and chlorophyll catabolites: functional comparisons with Atmrp1. Plant Cell. 1998;10:267-82 pubmed
- Joseph S, Bokkala S, Boehning D, Zeigler S. Factors determining the composition of inositol trisphosphate receptor hetero-oligomers expressed in COS cells. J Biol Chem. 2000;275:16084-90 pubmed..However, hetero-oligomers were synthesized with a longer lag period, suggesting that there may be kinetic constraints that favor homo-oligomers over hetero-oligomers. ..
- Zhao G, Song H, Chen Z, Mathews F, Jorns M. Monomeric sarcosine oxidase: role of histidine 269 in catalysis. Biochemistry. 2002;41:9751-64 pubmed
- Gonzalez E, Mongiat M, Slater S, Baffa R, Iozzo R. A novel interaction between perlecan protein core and progranulin: potential effects on tumor growth. J Biol Chem. 2003;278:38113-6 pubmed..Thus, progranulin/perlecan interaction could contribute to a fine regulation of tumor angiogenesis and could ultimately affect cancer growth. ..
- Zhang C, Perona J, Hou Y. Amino acid discrimination by a highly differentiated metal center of an aminoacyl-tRNA synthetase. Biochemistry. 2003;42:10931-7 pubmed..Most mutants are less attenuated in tRNA aminoacylation than in adenylate synthesis, suggesting that tRNA binding drives a conformational change to help assemble the active site. ..
- Sinha D, Badellino K, Marcinkiewicz M, Walsh P. Allosteric modification of factor XIa functional activity upon binding to polyanions. Biochemistry. 2004;43:7593-600 pubmed..These results provide compelling evidence that factor XIa binding to the polyanions, dextran sulfate and heparin, results in inhibition of the enzyme by an allosteric mechanism. ..
- Guild G, Connelly P, Ruggiero L, Vranich K, Tilney L. Actin filament bundles in Drosophila wing hairs: hairs and bristles use different strategies for assembly. Mol Biol Cell. 2005;16:3620-31 pubmed..Thus, bristle and hair cells use microvilli and cross-bridges to generate the common raw material of actin filament bundles but employ different strategies to assemble these into vastly different shapes. ..
- Lyubarsky A, Savchenko A, Morocco S, Daniele L, Redmond T, Pugh E. Mole quantity of RPE65 and its productivity in the generation of 11-cis-retinal from retinyl esters in the living mouse eye. Biochemistry. 2005;44:9880-8 pubmed
- Robertson S, Setty S, Sitaram A, Marks M, Lewis R, Chou M. Extracellular signal-regulated kinase regulates clathrin-independent endosomal trafficking. Mol Biol Cell. 2006;17:645-57 pubmed..These studies reveal a previously unappreciated link of Erk signaling to organelle dynamics and endosomal trafficking. ..
- Yamamoto N. Pathogenic significance of alpha-N-acetylgalactosaminidase activity found in the envelope glycoprotein gp160 of human immunodeficiency virus Type 1. AIDS Res Hum Retroviruses. 2006;22:262-71 pubmed..Therefore, Nagalase appears to play dual roles in viral infectivity and immunosuppression. ..
- Bhattacharji A, Kaplan B, Harris T, Qu X, Germann M, Covarrubias M. The concerted contribution of the S4-S5 linker and the S6 segment to the modulation of a Kv channel by 1-alkanols. Mol Pharmacol. 2006;70:1542-54 pubmed..Therefore, we propose that amphiphilic general anesthetic agents such as 1-alkanols may modulate gating of the Shaw2 K(+) channel by an interaction with its activation gate...
- Zhao G, Bruckner R, Jorns M. Identification of the oxygen activation site in monomeric sarcosine oxidase: role of Lys265 in catalysis. Biochemistry. 2008;47:9124-35 pubmed publisher..Significantly, the active sites for sarcosine oxidation and oxygen reduction are located on opposite faces of the flavin ring. ..
- Soslau G, Prest P, Class R, Jost M, Mathews L. Inhibition of gamma-thrombin-induced human platelet aggregation by histone H1subtypes and H1.3 fragments. Platelets. 2009;20:349-56 pubmed publisher..1 fragment further enhanced its inhibitory activity. These studies may help to develop specific drugs to differentially inhibit the platelet thrombin receptors. ..
- Moore D, Nygren P, Jo H, Boesze Battaglia K, Bennett J, Degrado W. Affinity of talin-1 for the ?3-integrin cytosolic domain is modulated by its phospholipid bilayer environment. Proc Natl Acad Sci U S A. 2012;109:793-8 pubmed publisher..Instead, the interactions appear to be tuned in such a way that the equilibrium between inactive and active integrin conformations can be readily regulated. ..
- Kuo Y, Andrews A. Quantitating the specificity and selectivity of Gcn5-mediated acetylation of histone H3. PLoS ONE. 2013;8:e54896 pubmed publisher..These data provide a first look at quantitating the specificity and selectivity of multiple lysines on a single substrate (H3) by Gcn5. ..
- Hu W, Kan Z, Mayne L, Englander S. Cytochrome c folds through foldon-dependent native-like intermediates in an ordered pathway. Proc Natl Acad Sci U S A. 2016;113:3809-14 pubmed publisher..The present results also emphasize the condition-dependent nature of kinetic barriers, which, with less informative experimental methods (fluorescence, etc.), are often confused with variability in intermediates and pathways. ..
- Po P, Delaney E, Gamper H, Szantai Kis D, Speight L, Tu L, et al. Effect of Nascent Peptide Steric Bulk on Elongation Kinetics in the Ribosome Exit Tunnel. J Mol Biol. 2017;429:1873-1888 pubmed publisher..We report here that besides PTC events, translation kinetics depend on steric constraints on nascent peptide side chains and that confined movements of cramped side chains within and through the tunnel fine-tune elongation rates. ..
- Duda T, Goraczniak R, Sitaramayya A, Sharma R. Cloning and expression of an ATP-regulated human retina C-type natriuretic factor receptor guanylate cyclase. Biochemistry. 1993;32:1391-5 pubmed..We now report the molecular cloning and expression of CNP-RGC from human retina and show that ATP is obligatory in CNP signaling also.(ABSTRACT TRUNCATED AT 250 WORDS) ..
- Wu V, Cohen M. Evidence for a ligand receptor system mediating the biologic effects of glycated albumin in glomerular mesangial cells. Biochem Biophys Res Commun. 1995;207:521-8 pubmed
- Kume H, Hall I, Washabau R, Takagi K, Kotlikoff M. Beta-adrenergic agonists regulate KCa channels in airway smooth muscle by cAMP-dependent and -independent mechanisms. J Clin Invest. 1994;93:371-9 pubmed..We conclude that beta-adrenergic stimulation of KCa channel activity and relaxation of tone in airway smooth muscle occurs, in part, by means independent of cyclic AMP formation. ..
- Sell C, Dumenil G, Deveaud C, Miura M, Coppola D, DeAngelis T, et al. Effect of a null mutation of the insulin-like growth factor I receptor gene on growth and transformation of mouse embryo fibroblasts. Mol Cell Biol. 1994;14:3604-12 pubmed..The signaling pathways participating in cell growth and transformation are discussed on the basis of these results. ..
- Lounsbury K, Schlegel B, Poncz M, Brass L, Manning D. Analysis of Gz alpha by site-directed mutagenesis. Sites and specificity of protein kinase C-dependent phosphorylation. J Biol Chem. 1993;268:3494-8 pubmed..Moreover, Gi alpha, Gz alpha, and Gq alpha were not phosphorylated in response to analogues of cAMP or cGMP. Thus, only Gz alpha is phosphorylated in platelets and only in response to activation of protein kinase C. ..
- Mather M, Rottenberg H. Intrinsic uncoupling of cytochrome c oxidase may cause the maternally inherited mitochondrial diseases MELAS and LHON. FEBS Lett. 1998;433:93-7 pubmed..These results indicate that both substitutions (F263L > A212T) cause intrinsic uncoupling, which may be the direct cause of the diseases. These results also suggest that subunit III is involved in proton pumping...
- Taylor J, Markham G. The bifunctional active site of s-adenosylmethionine synthetase. Roles of the active site aspartates. J Biol Chem. 1999;274:32909-14 pubmed..This indicates that the Asp-271 side chain size is a discriminator between the activating ability of Ca(2+) and the smaller Mg(2+). ..
- Li J, Saxena S, Pain D, Dancis A. Adrenodoxin reductase homolog (Arh1p) of yeast mitochondria required for iron homeostasis. J Biol Chem. 2001;276:1503-9 pubmed..Thus, Arh1p is required for control of cellular and mitochondrial iron levels and for the activities of Fe-S cluster proteins. ..
- Jensen L, Whitehead A. IRAK1b, a novel alternative splice variant of interleukin-1 receptor-associated kinase (IRAK), mediates interleukin-1 signaling and has prolonged stability. J Biol Chem. 2001;276:29037-44 pubmed..The presence of an alternative splice variant of IRAK1, which is functionally active and highly stable following IL-1 stimulation, adds further complexity to the control mechanisms that govern IL-1 signaling. ..
- Darrouzet E, Daldal F. Movement of the iron-sulfur subunit beyond the ef loop of cytochrome b is required for multiple turnovers of the bc1 complex but not for single turnover Qo site catalysis. J Biol Chem. 2002;277:3471-6 pubmed
- Westhoff C, Ferreri Jacobia M, Mak D, Foskett J. Identification of the erythrocyte Rh blood group glycoprotein as a mammalian ammonium transporter. J Biol Chem. 2002;277:12499-502 pubmed..14 mm) consistent with the hypothesis that the uptake of MA and ammonium involves a similar H(+)-coupled counter-transport mechanism. ..
- Allman D, Sambandam A, Kim S, Miller J, Pagan A, Well D, et al. Thymopoiesis independent of common lymphoid progenitors. Nat Immunol. 2003;4:168-74 pubmed..We conclude that ETPs can develop via a CLP-independent pathway. ..
- Li J, Sheng S, Perry C, Kleyman T. Asymmetric organization of the pore region of the epithelial sodium channel. J Biol Chem. 2003;278:13867-74 pubmed..Our results, placed in context with previous observations, suggest that pore regions from the three ENaC subunits have an asymmetric organization. ..
- McDonnell M, Li H, Funk C. Characterization of epidermal 12(S) and 12(R) lipoxygenases. Adv Exp Med Biol. 2002;507:147-53 pubmed
- Bates S, Tao J, Notarfrancesco K, DeBolt K, Shuman H, Fisher A. Effect of surfactant protein A on granular pneumocyte surfactant secretion in vitro. Am J Physiol Lung Cell Mol Physiol. 2003;285:L1055-65 pubmed..Finally, there was no evidence of a large PL pool retained on the cell surface through interaction with SP-A. Thus SP-A exposure does not affect these stages in the surfactant secretory pathway of type II cells. ..
- Toso R, Camire R. Removal of B-domain sequences from factor V rather than specific proteolysis underlies the mechanism by which cofactor function is realized. J Biol Chem. 2004;279:21643-50 pubmed