Genomes and Genes
Experts and Doctors on tertiary protein structure in Durham, North Carolina, United States
Locale: Durham, North Carolina, United States
Topic: tertiary protein structure
Publications104 found, 100 shown here
- Ward R, Schweizer L, Lamb R, Fehon R. The protein 4.1, ezrin, radixin, moesin (FERM) domain of Drosophila Coracle, a cytoplasmic component of the septate junction, provides functions essential for embryonic development and imaginal cell proliferation. Genetics. 2001;159:219-28 pubmed..This result suggests the presence of a second functional domain within Coracle, a notion supported by molecular characterization of a series of coracle alleles. ..
- Shaffer P, Gewirth D. Structural basis of VDR-DNA interactions on direct repeat response elements. EMBO J. 2002;21:2242-52 pubmed..Selective structure-based mutations in the asymmetric homodimeric interface result in a VDR DBD protein that is defective in homodimerization but now forms heterodimers with the 9-cis retinoic acid receptor (RXR) DBD. ..
- Weidman J, Maloney K, Jirtle R. Comparative phylogenetic analysis reveals multiple non-imprinted isoforms of opossum Dlk1. Mamm Genome. 2006;17:157-67 pubmed..These findings clearly demonstrate that imprinted genes did not all evolve before the divergence of marsupials and eutherians. ..
- Murphy S, Huang Z, Wen Y, Spillman M, Whitaker R, Simel L, et al. Frequent IGF2/H19 domain epigenetic alterations and elevated IGF2 expression in epithelial ovarian cancer. Mol Cancer Res. 2006;4:283-92 pubmed..These data indicate that the epigenetic changes observed in these cancers at the imprint center may contribute to IGF2 overexpression in a novel mechanistic manner. ..
- Gaillard S, Dwyer M, McDonnell D. Definition of the molecular basis for estrogen receptor-related receptor-alpha-cofactor interactions. Mol Endocrinol. 2007;21:62-76 pubmed
- Nichols J, Xiang S, Schindelin H, Rajagopalan K. Mutational analysis of Escherichia coli MoeA: two functional activities map to the active site cleft. Biochemistry. 2007;46:78-86 pubmed..On the basis of these results, two different functional areas were assigned to regions at or near the MoeA active site cleft. ..
- Heymann J, Weaver K, Mietzner T, Crumbliss A. Sulfate as a synergistic anion facilitating iron binding by the bacterial transferrin FbpA: the origins and effects of anion promiscuity. J Am Chem Soc. 2007;129:9704-12 pubmed..This structural and thermodynamic analysis of anion binding in FeFbpA-X provides additional insight into anion promiscuity and importance. ..
- Bogerd H, Tallmadge R, Oaks J, Carpenter S, Cullen B. Equine infectious anemia virus resists the antiretroviral activity of equine APOBEC3 proteins through a packaging-independent mechanism. J Virol. 2008;82:11889-901 pubmed publisher..It therefore appears that EIAV has evolved a novel mechanism to specifically neutralize the biological activities of the cognate eA3F1 and eA3F2 proteins at a step subsequent to virion incorporation...
- Kozubowski L, Saito K, Johnson J, Howell A, Zyla T, Lew D. Symmetry-breaking polarization driven by a Cdc42p GEF-PAK complex. Curr Biol. 2008;18:1719-26 pubmed publisher..Our findings provide mechanistic insight into an evolutionarily conserved pattern-forming positive-feedback pathway. ..
- Cuneo M, Beese L, Hellinga H. Structural analysis of semi-specific oligosaccharide recognition by a cellulose-binding protein of thermotoga maritima reveals adaptations for functional diversification of the oligopeptide periplasmic binding protein fold. J Biol Chem. 2009;284:33217-23 pubmed publisher..Semi-specific recognition, in which a molecular class rather than individual species is selected, provides an efficient solution for the uptake of complex mixtures...
- Nimjee S, Oney S, Volovyk Z, Bompiani K, Long S, Hoffman M, et al. Synergistic effect of aptamers that inhibit exosites 1 and 2 on thrombin. RNA. 2009;15:2105-11 pubmed publisher..This combination of exosite 1 and exosite 2 inhibitors may provide a particularly effective antithrombotic approach. ..
- Gallione C, Aylsworth A, Beis J, Berk T, Bernhardt B, Clark R, et al. Overlapping spectra of SMAD4 mutations in juvenile polyposis (JP) and JP-HHT syndrome. Am J Med Genet A. 2010;152A:333-9 pubmed publisher..In conclusion, a patient who tests positive for any SMAD4 mutation must be considered at risk for the combined syndrome of JP-HHT and monitored accordingly. ..
- Bloor J, Kiehart D. zipper Nonmuscle myosin-II functions downstream of PS2 integrin in Drosophila myogenesis and is necessary for myofibril formation. Dev Biol. 2001;239:215-28 pubmed publisher..We suggest that nonmuscle myosin-II functions at the muscle termini and the Z-line as an actin crosslinker and acts to maintain the structural integrity of the sarcomere...
- Mohler P, Davis J, Davis L, Hoffman J, Michaely P, Bennett V. Inositol 1,4,5-trisphosphate receptor localization and stability in neonatal cardiomyocytes requires interaction with ankyrin-B. J Biol Chem. 2004;279:12980-7 pubmed..These new results provide the first physiological evidence of a molecular partner required for early post-translational stability of InsP(3)R. ..
- Ray B, Lee N, How T, Blobe G. ALK5 phosphorylation of the endoglin cytoplasmic domain regulates Smad1/5/8 signaling and endothelial cell migration. Carcinogenesis. 2010;31:435-41 pubmed publisher..Taken together, these results support endoglin phosphorylation by ALK5 as an important mechanism for regulating TGF-beta superfamily signaling and migration in endothelial cells. ..
- Golden B, Ramakrishnan V, White S. Ribosomal protein L6: structural evidence of gene duplication from a primitive RNA binding protein. EMBO J. 1993;12:4901-8 pubmed..The domain structure is homologous with several other ribosomal proteins and to a large family of eukaryotic RNA binding proteins...
- Breazeale S, Ribeiro A, McClerren A, Raetz C. A formyltransferase required for polymyxin resistance in Escherichia coli and the modification of lipid A with 4-Amino-4-deoxy-L-arabinose. Identification and function oF UDP-4-deoxy-4-formamido-L-arabinose. J Biol Chem. 2005;280:14154-67 pubmed..Because the L-Ara4N unit attached to lipid A is not derivatized with a formyl group, we postulate the existence of a deformylase, acting later in the pathway. ..
- Reynolds C, Kalb S, Cotter R, Raetz C. A phosphoethanolamine transferase specific for the outer 3-deoxy-D-manno-octulosonic acid residue of Escherichia coli lipopolysaccharide. Identification of the eptB gene and Ca2+ hypersensitivity of an eptB deletion mutant. J Biol Chem. 2005;280:21202-11 pubmed..Based on its sequence similarity to EptA(PmrC), we propose that the active site of EptB faces the periplasmic surface of the inner membrane. ..
- Hsu C, Kikuchi K, Kondo M. Activation of mitogen-activated protein kinase kinase (MEK)/extracellular signal regulated kinase (ERK) signaling pathway is involved in myeloid lineage commitment. Blood. 2007;110:1420-8 pubmed..These results demonstrate that activation of MEK/ERK plays an important role in GM lineage commitment. ..
- Kizhatil K, Wu Y, Sen A, Bennett V. A new activity of doublecortin in recognition of the phospho-FIGQY tyrosine in the cytoplasmic domain of neurofascin. J Neurosci. 2002;22:7948-58 pubmed..The finding that doublecortin associates with FIGQY-phosphorylated neurofascin provides the first connection of doublecortin with the plasma membrane and could be important for a function of doublecortin in directing neuronal migration. ..
- Il ichev Y, Perry J, Ruker F, Dockal M, Simon J. Interaction of ochratoxin A with human serum albumin. Binding sites localized by competitive interactions with the native protein and its recombinant fragments. Chem Biol Interact. 2002;141:275-93 pubmed..9 x 10(5) M(-1), was smaller than the largest constant for HSA by a factor of approximately 7. The binding constant for OTA with D3, 1.1 x 10(5) M(-1), was very close to that of the secondary binding site for HSA. ..
- Radin J, Grass S, Meng G, Cotter S, Waksman G, St Geme J. Structural basis for the differential binding affinities of the HsfBD1 and HsfBD2 domains in the Haemophilus influenzae Hsf adhesin. J Bacteriol. 2009;191:5068-75 pubmed publisher..This work demonstrates the critical role of a single amino acid in the core of the binding pocket in determining the relative affinities of the HsfBD1 and HsfBD2 binding domains. ..
- Pai K, Bussiere D, Wang F, White S, Bastia D. Structure of the replication terminus-terminator protein complex as probed by affinity cleavage. Proc Natl Acad Sci U S A. 1996;93:10647-52 pubmed..Using these contacts as distance constraints, with the crystal structure of RTP, we have constructed a model of the DNA-protein complex. The biological implications of the model have been discussed. ..
- Shenoy S, Lefkowitz R. Trafficking patterns of beta-arrestin and G protein-coupled receptors determined by the kinetics of beta-arrestin deubiquitination. J Biol Chem. 2003;278:14498-506 pubmed..Thus the ubiquitination status of beta-arrestin determines the stability of the receptor-beta-arrestin complex as well as the trafficking pattern of beta-arrestin. ..
- Cho K, Yi H, Desai R, Hand A, Haas A, Ferreira P. RANBP2 is an allosteric activator of the conventional kinesin-1 motor protein, KIF5B, in a minimal cell-free system. EMBO Rep. 2009;10:480-6 pubmed publisher..Hence, RANBP2 is the first native and positive allosteric activator known to jump-start and boost directly the activity of a kinesin. ..
- Romac J, Graff D, Keene J. The U1 small nuclear ribonucleoprotein (snRNP) 70K protein is transported independently of U1 snRNP particles via a nuclear localization signal in the RNA-binding domain. Mol Cell Biol. 1994;14:4662-70 pubmed
- Chen W, Kirkbride K, How T, Nelson C, Mo J, Frederick J, et al. Beta-arrestin 2 mediates endocytosis of type III TGF-beta receptor and down-regulation of its signaling. Science. 2003;301:1394-7 pubmed..Thus, the regulatory actions of beta-arrestins are broader than previously appreciated, extending to the TGF-beta receptor family as well. ..
- Rhoads D, Brunner Neuenschwander B, Levings C, Siedow J. Cross-linking and disulfide bond formation of introduced cysteine residues suggest a modified model for the tertiary structure of URF13 in the pore-forming oligomers. Arch Biochem Biophys. 1998;354:158-64 pubmed..Sequential cross-linking of URF13-R81C oligomers with bismaleimidohexane (Cys-Cys cross-linker) and N,N'-dicyclohexylcarbodiimide (Lys-Asp/Glu cross-linker) suggests that URF13 oligomers consist of an even number of monomers. ..
- Li X, McDonnell D. The transcription factor B-Myb is maintained in an inhibited state in target cells through its interaction with the nuclear corepressors N-CoR and SMRT. Mol Cell Biol. 2002;22:3663-73 pubmed..Cumulatively, these findings suggest that the nuclear corepressors N-CoR and SMRT serve a previously unappreciated role as regulators of B-Myb transcriptional activity. ..
- Peng W, Quinn Allen M, Kim S, Alexander K, Kane W. Trp2063 and Trp2064 in the factor Va C2 domain are required for high-affinity binding to phospholipid membranes but not for assembly of the prothrombinase complex. Biochemistry. 2004;43:4385-93 pubmed..It remains to be determined whether occupancy of this PS binding site in factor Va is also required for high-affinity binding to factor Xa. ..
- Gitler D, Xu Y, Kao H, Lin D, Lim S, Feng J, et al. Molecular determinants of synapsin targeting to presynaptic terminals. J Neurosci. 2004;24:3711-20 pubmed..Domain D inhibited targeting, but this inhibition was overcome by domain E. Thus, multiple intermolecular and intramolecular interactions are required for synapsins to target to presynaptic terminals. ..
- Yang J, Bogerd H, Wang P, Page D, Cullen B. Two closely related human nuclear export factors utilize entirely distinct export pathways. Mol Cell. 2001;8:397-406 pubmed..Thus, while the related TAP and NXF3 proteins are both capable of mediating nuclear RNA export, they do so via unrelated export pathways...
- Peng W, Quinn Allen M, Kane W. Mutation of hydrophobic residues in the factor Va C1 and C2 domains blocks membrane-dependent prothrombin activation. J Thromb Haemost. 2005;3:351-4 pubmed
- Edwards K, Chang X, Kiehart D. Essential light chain of Drosophila nonmuscle myosin II. J Muscle Res Cell Motil. 1995;16:491-8 pubmed..The gene encoding Drosophila nonmuscle essential light chain (Mlc-c) localizes to cytological position 5A6 and we discuss prospects for genetic analysis in this region. ..
- Morris D, Phatnani H, Greenleaf A. Phospho-carboxyl-terminal domain binding and the role of a prolyl isomerase in pre-mRNA 3'-End formation. J Biol Chem. 1999;274:31583-7 pubmed..This process may be linked to a variety of pre-mRNA maturation events that use the phosphorylated CTD, including the coupled processes of pre-mRNA 3'-end formation and transcription termination. ..
- Miller W, McDonald P, Cai S, Field M, Davis R, Lefkowitz R. Identification of a motif in the carboxyl terminus of beta -arrestin2 responsible for activation of JNK3. J Biol Chem. 2001;276:27770-7 pubmed
- Raetz C, Whitfield C. Lipopolysaccharide endotoxins. Annu Rev Biochem. 2002;71:635-700 pubmed..The expanding knowledge of TLR4 specificity and its downstream signaling pathways should provide new opportunities for blocking inflammation associated with infection...
- Naga Prasad S, Laporte S, Chamberlain D, Caron M, Barak L, Rockman H. Phosphoinositide 3-kinase regulates beta2-adrenergic receptor endocytosis by AP-2 recruitment to the receptor/beta-arrestin complex. J Cell Biol. 2002;158:563-75 pubmed
- Marchuk D, Srinivasan S, Squire T, Zawistowski J. Vascular morphogenesis: tales of two syndromes. Hum Mol Genet. 2003;12 Spec No 1:R97-112 pubmed..Emerging but intriguing tales are being told by the genes (and their protein products) mutated in these disorders. ..
- Mattiske D, Kume T, Hogan B. The mouse forkhead gene Foxc1 is required for primordial germ cell migration and antral follicle development. Dev Biol. 2006;290:447-58 pubmed..We conclude that alongside its previously known roles in kidney, cardiovascular and eye development, Foxc1 has essential functions during at least two stages of gonad development-germ cell migration and folliculogenesis. ..
- Lane K, Beese L. Thematic review series: lipid posttranslational modifications. Structural biology of protein farnesyltransferase and geranylgeranyltransferase type I. J Lipid Res. 2006;47:681-99 pubmed..Such complexes may assist in the design of inhibitors that could lead to treatments for cancer, viral infection, and a number of deadly parasitic diseases. ..
- Pai M, Tzeng S, Kovacs J, Keaton M, Li S, Yao T, et al. Solution structure of the Ubp-M BUZ domain, a highly specific protein module that recognizes the C-terminal tail of free ubiquitin. J Mol Biol. 2007;370:290-302 pubmed..The unique ubiquitin-recognition mode of the BUZ domain family suggests that they may function as "sensors" of free ubiquitin in cells to achieve regulatory roles in many aspects of ubiquitin-dependent processes. ..
- Bartling C, Raetz C. Steady-state kinetics and mechanism of LpxD, the N-acyltransferase of lipid A biosynthesis. Biochemistry. 2008;47:5290-302 pubmed publisher..Mutagenesis implicates E. coli H239 but excludes H276 as the catalytic base, and neither residue is likely to stabilize the oxyanion intermediate. ..
- Lee N, Haney J, Sogani J, Blobe G. Casein kinase 2beta as a novel enhancer of activin-like receptor-1 signaling. FASEB J. 2009;23:3712-21 pubmed publisher..Similarly, ALK-1 and CK2beta antagonized endothelial tubule formation in Matrigel. These studies support CK2beta as an important regulator of ALK-1 signaling and ALK-1-mediated functions in endothelial cells. ..
- Lee D, Ohn T, Chiang Y, Quigley G, Yao G, Liu Y, et al. PUF3 acceleration of deadenylation in vivo can operate independently of CCR4 activity, possibly involving effects on the PAB1-mRNP structure. J Mol Biol. 2010;399:562-75 pubmed publisher..PUF3 appears, therefore, both to act independently of CCR4 activity, possibly through effects on PAB1-mRNP structure, and to be capable of retaining the CCR4-NOT complex. ..
- Guo H, Gao C, Mi Z, Zhang J, Kuo P. Characterization of the PC4 binding domain and its interactions with HNF4alpha. J Biochem. 2007;141:635-40 pubmed..Our results indicate that the presence of the IR state enhances PC4-HNF4 binding to upregulate transcription of target hepatocyte genes, such as iNOS. ..
- Mulugu S, Bai W, Fridy P, Bastidas R, Otto J, Dollins D, et al. A conserved family of enzymes that phosphorylate inositol hexakisphosphate. Science. 2007;316:106-9 pubmed..Alterations in kinase activity led to defects in cell growth, morphology, and interactions with ARP complex members. The functionality of Asp1 and Vip1 may provide cells with increased signaling capacity through metabolism of IP6. ..
- Marion S, Fralish G, Laporte S, Caron M, Barak L. N-terminal tyrosine modulation of the endocytic adaptor function of the beta-arrestins. J Biol Chem. 2007;282:18937-44 pubmed..Additionally, these naturally occurring variations in beta-arrestins may also differentially regulate the composition of the signaling complexes organized on the receptor. ..
- Crutchley J, King K, Keaton M, Szkotnicki L, Orlando D, Zyla T, et al. Molecular dissection of the checkpoint kinase Hsl1p. Mol Biol Cell. 2009;20:1926-36 pubmed publisher..We suggest that Hsl1p responds to alterations in septin organization, which themselves occur in response to the local geometry of the cell cortex. ..
- Hu L, Chen W, Premont R, Cong M, Lefkowitz R. G protein-coupled receptor kinase 5 regulates beta 1-adrenergic receptor association with PSD-95. J Biol Chem. 2002;277:1607-13 pubmed..Regulation of protein association through receptor phosphorylation may be a general mechanism used by G protein-coupled receptors that associate via PDZ domain-mediated protein/protein interactions. ..
- Taylor J, Reid T, Terry K, Casey P, Beese L. Structure of mammalian protein geranylgeranyltransferase type-I. EMBO J. 2003;22:5963-74 pubmed..Understanding these key features of specificity is expected to contribute to optimization of anti-cancer and anti-parasite drugs. ..
- Persaud Sawin D, McNamara J, Rylova S, VanDongen A, Boustany R. A galactosylceramide binding domain is involved in trafficking of CLN3 from Golgi to rafts via recycling endosomes. Pediatr Res. 2004;56:449-63 pubmed..These studies identify a novel CLN3 domain that may dictate localization and function of CLN3. ..
- Nishiki T, Augustine G. Dual roles of the C2B domain of synaptotagmin I in synchronizing Ca2+-dependent neurotransmitter release. J Neurosci. 2004;24:8542-50 pubmed
- Zhao X, Sternsdorf T, Bolger T, Evans R, Yao T. Regulation of MEF2 by histone deacetylase 4- and SIRT1 deacetylase-mediated lysine modifications. Mol Cell Biol. 2005;25:8456-64 pubmed..Our studies reveal a novel regulation of MEF2 transcriptional activity by two distinct classes of deacetylases that affect MEF2 sumoylation and acetylation. ..
- Schmalzigaug R, Garron M, Roseman J, Xing Y, Davidson C, Arold S, et al. GIT1 utilizes a focal adhesion targeting-homology domain to bind paxillin. Cell Signal. 2007;19:1733-44 pubmed..Thus, despite apparent structural similarity in their FAT domains, GIT1 and FAK binding to paxillin is differentially regulated. ..
- Torres G, Yao W, Mohn A, Quan H, Kim K, Levey A, et al. Functional interaction between monoamine plasma membrane transporters and the synaptic PDZ domain-containing protein PICK1. Neuron. 2001;30:121-34 pubmed..These findings indicate a role for PDZ-mediated protein interactions in the localization, expression, and function of monoamine transporters. ..
- Osawa M, Erickson H. FtsZ from divergent foreign bacteria can function for cell division in Escherichia coli. J Bacteriol. 2006;188:7132-40 pubmed..The C-terminal peptide of FtsZ, which binds FtsA, provides the link between FtsZ assembly and peptidoglycan remodeling...
- Boczonadi V, McInroy L, Määttä A. Cytolinker cross-talk: periplakin N-terminus interacts with plectin to regulate keratin organisation and epithelial migration. Exp Cell Res. 2007;313:3579-91 pubmed..Finally, ablation of either periplakin or plectin, or both proteins simultaneously, impaired migration of MCF-7 epithelial sheets. Thus, we have identified a novel functional co-localisation between two plakin cytolinker proteins. ..
- Soderling S. Grab your partner with both hands: cytoskeletal remodeling by Arp2/3 signaling. Sci Signal. 2009;2:pe5 pubmed publisher..Emerging data on an important regulator of actin dynamics, the actin-related protein 2 and 3 (Arp2/3) complex, suggest a model of how this may occur. ..
- Waters J, O Neal W, White K, Wakeford C, Lansdon E, Harris J, et al. Mutations in the thumb-connection and RNase H domain of HIV type-1 reverse transcriptase of antiretroviral treatment-experienced patients. Antivir Ther. 2009;14:231-9 pubmed
- Misra U, Kaczowka S, Pizzo S. Inhibition of NF-kappaB1 and NF-kappaB2 activation in prostate cancer cells treated with antibody against the carboxyl terminal domain of GRP78: effect of p53 upregulation. Biochem Biophys Res Commun. 2010;392:538-42 pubmed publisher..These studies demonstrate that antibody directed against the carboxyl terminal domain of GRP78 inhibits the pro-proliferative NF-kappaB signaling cascade in cancer cells. ..
- Sato S, Miller A, Howard M, Tedder T. Regulation of B lymphocyte development and activation by the CD19/CD21/CD81/Leu 13 complex requires the cytoplasmic domain of CD19. J Immunol. 1997;159:3278-87 pubmed..Moreover, expression of the CD19 cytoplasmic domain is required for optimal signaling through the B cell Ag receptor complex. ..
- Ohashi T, Erickson H. The disulfide bonding pattern in ficolin multimers. J Biol Chem. 2004;279:6534-9 pubmed..We present a model in which symmetric Cys24-Cys24 disulfide bonds between trimers are the basis for multimerization. The model may also be relevant to collectin multimers. ..
- Rosser M, Trotta B, Marshall M, Berwin B, Nicchitta C. Adenosine nucleotides and the regulation of GRP94-client protein interactions. Biochemistry. 2004;43:8835-45 pubmed..On the basis of these data, we propose that structural maturation of the client protein substrate, rather than ATP binding or hydrolysis, serves as the primary signal for dissociation of GRP94-client protein complexes. ..
- Deming P, Schafer Z, Tashker J, Potts M, Deshmukh M, Kornbluth S. Bcr-Abl-mediated protection from apoptosis downstream of mitochondrial cytochrome c release. Mol Cell Biol. 2004;24:10289-99 pubmed..These data provide a novel locus of Bcr-Abl antiapoptotic action and suggest a distinct mechanism of apoptosomal inhibition. ..
- Arcasoy M, Karayal A. Erythropoietin hypersensitivity in primary familial and congenital polycythemia: role of tyrosines Y285 and Y344 in erythropoietin receptor cytoplasmic domain. Biochim Biophys Acta. 2005;1740:17-28 pubmed..These findings suggest that both tyrosine residues Y285 and Y344 in the cytoplasmic domain of EPOR-ME may contribute to increased Epo sensitivity that is characteristic of PFCP phenotype. ..
- Natalizio B, Robson Dixon N, Garcia Blanco M. The Carboxyl-terminal Domain of RNA Polymerase II Is Not Sufficient to Enhance the Efficiency of Pre-mRNA Capping or Splicing in the Context of a Different Polymerase. J Biol Chem. 2009;284:8692-702 pubmed publisher..We propose that the efficient coupling of transcription to pre-mRNA processing requires not only the phosphorylated CTD but also other RNAP II specific subunits or associated factors. ..
- Bomar M, D Souza S, Bienko M, Dikic I, Walker G, Zhou P. Unconventional ubiquitin recognition by the ubiquitin-binding motif within the Y family DNA polymerases iota and Rev1. Mol Cell. 2010;37:408-17 pubmed publisher..Point mutations disrupting the UBM-ubiquitin interaction also impair the accumulation of pol iota in replication foci and Rev1-mediated DNA damage tolerance in vivo. ..
- Halsell S, Chu B, Kiehart D. Genetic analysis demonstrates a direct link between rho signaling and nonmuscle myosin function during Drosophila morphogenesis. Genetics. 2000;155:1253-65 pubmed..Our studies provide direct genetic proof of the biological relevance of regulation of myosin by Rho signal transduction in an intact metazoan. ..
- Wuebbens M, Liu M, Rajagopalan K, Schindelin H. Insights into molybdenum cofactor deficiency provided by the crystal structure of the molybdenum cofactor biosynthesis protein MoaC. Structure. 2000;8:709-18 pubmed..This residue is located near the N-terminal end of helix alpha4 at an interface between two monomers. The MoaC structure provides a framework for the analysis of additional dysfunctional mutations in the corresponding human gene. ..
- Cook J, Chasse D, Nevins J. The regulated association of Cdt1 with minichromosome maintenance proteins and Cdc6 in mammalian cells. J Biol Chem. 2004;279:9625-33 pubmed
- Svenson I, Kloos M, Gaskell P, Nance M, Garbern J, Hisanaga S, et al. Intragenic modifiers of hereditary spastic paraplegia due to spastin gene mutations. Neurogenetics. 2004;5:157-64 pubmed..Our identification of S44L and P45Q as modifiers of the HSP phenotype suggests a role for spastin phosphorylation by Cdks in the neurodegeneration of the most-common form of HSP. ..
- Schäfer A, Bogerd H, Cullen B. Specific packaging of APOBEC3G into HIV-1 virions is mediated by the nucleocapsid domain of the gag polyprotein precursor. Virology. 2004;328:163-8 pubmed..Surprisingly, RNA was also found to be essential for formation of the nucleocapsid--APOBEC3G complex in vitro, thus raising the possibility that RNA may form a bridge between these two proteins...
- Williams A, Raetz C. Structural basis for the acyl chain selectivity and mechanism of UDP-N-acetylglucosamine acyltransferase. Proc Natl Acad Sci U S A. 2007;104:13543-50 pubmed..0-A structure of LpxA with bound UDP-GlcNAc. Our structures show how LpxA selects for 14-carbon R-3-hydroxyacyl chains and reveal two modes of UDP binding. ..
- Wharton R, Sonoda J, Lee T, Patterson M, Murata Y. The Pumilio RNA-binding domain is also a translational regulator. Mol Cell. 1998;1:863-72 pubmed..We also show that Nos and Pum can repress cap-independent translation from an internal ribosome entry site (IRES) in vivo, suggesting that they act downstream of the initial steps of normal, cap-dependent translation. ..
- Coburn G, Wiegand H, Kang Y, Ho D, Georgiadis M, Cullen B. Using viral species specificity to define a critical protein/RNA interaction surface. Genes Dev. 2001;15:1194-205 pubmed..These data emphasize the potential importance of cross-species genetic complementation in the identification and characterization of cellular factors that are critical for different aspects of viral replication...
- Breazeale S, Ribeiro A, Raetz C. Oxidative decarboxylation of UDP-glucuronic acid in extracts of polymyxin-resistant Escherichia coli. Origin of lipid a species modified with 4-amino-4-deoxy-L-arabinose. J Biol Chem. 2002;277:2886-96 pubmed..ArnA resembles UDP-galactose epimerase, dTDP-glucose-4,6-dehydratase, and UDP-xylose synthase in oxidizing the C-4" position of its substrate, but differs in that it releases the NADH product. ..
- Martik D, Baitinger C, Modrich P. Differential specificities and simultaneous occupancy of human MutSalpha nucleotide binding sites. J Biol Chem. 2004;279:28402-10 pubmed..These findings imply that MutSalpha has two nucleotide binding sites with differential specificities for ADP and ATP and suggest that the ADP.MutSalpha.ATP ternary complex has an important role in mismatch repair. ..
- Dill A, Thomas S, Hu J, Steber C, Sun T. The Arabidopsis F-box protein SLEEPY1 targets gibberellin signaling repressors for gibberellin-induced degradation. Plant Cell. 2004;16:1392-405 pubmed..This effect of sly1-d appears to be caused by an enhanced interaction between sly1-d and the DELLA proteins. ..
- Freibaum B, Counter C. hSnm1B is a novel telomere-associated protein. J Biol Chem. 2006;281:15033-6 pubmed..Furthermore, the C terminus of hSnm1B was shown to interact with the TRF homology domain of TRF2 indicating that hSnm1B is likely recruited to the telomere via interaction with the double-stranded telomere-binding protein TRF2. ..
- Stemmle L, Fields T, Casey P. The regulator of G protein signaling domain of axin selectively interacts with Galpha12 but not Galpha13. Mol Pharmacol. 2006;70:1461-8 pubmed..These findings establish that the RGS domain of axin is able to directly interact with the alpha subunit of heterotrimeric G protein G12 and provide a unique tool to interdict Galpha12-mediated signaling processes. ..
- Kizhatil K, Davis J, Davis L, Hoffman J, Hogan B, Bennett V. Ankyrin-G is a molecular partner of E-cadherin in epithelial cells and early embryos. J Biol Chem. 2007;282:26552-61 pubmed..Biol. Chem. 282, 2029-2037 ). Together with the current findings, these data suggest a ankyrin/spectrin-based mechanism for coordinating membrane assembly with extracellular interactions of E-cadherin at sites of cell-cell contact. ..
- Kadyrov F, Holmes S, Arana M, Lukianova O, O Donnell M, Kunkel T, et al. Saccharomyces cerevisiae MutLalpha is a mismatch repair endonuclease. J Biol Chem. 2007;282:37181-90 pubmed..yMutSalpha.heteroduplex ternary complex. The loaded form of yPCNA may play an important effector role in directing yMutLalpha incision to the discontinuous strand of a nicked heteroduplex. ..
- Zaas D, Swan Z, Brown B, Li G, Randell S, Degan S, et al. Counteracting signaling activities in lipid rafts associated with the invasion of lung epithelial cells by Pseudomonas aeruginosa. J Biol Chem. 2009;284:9955-64 pubmed publisher..The success of Pseudomonas in co-opting lipid raft-mediated endocytosis to invade lung epithelial cells may depend on the relative strengths of these counteracting signaling activities. ..
- Wu J, Phatnani H, Hsieh T, Greenleaf A. The phosphoCTD-interacting domain of Topoisomerase I. Biochem Biophys Res Commun. 2010;397:117-9 pubmed publisher..The long-known in vivo association of Topo I with active genes presumably can be attributed, wholly or in part, to the TRI domain-mediated binding of Topo I to the phosphoCTD of transcribing RNAPII. ..
- Liang Y, Tedder T. Identification of a CD20-, FcepsilonRIbeta-, and HTm4-related gene family: sixteen new MS4A family members expressed in human and mouse. Genomics. 2001;72:119-27 pubmed..1 along with CD20, FcepsilonRIbeta, and HTm4. Thus, like CD20 and FcepsilonRIbeta, the other MS4A family members are likely to be components of oligomeric cell surface complexes that serve diverse signal transduction functions. ..
- Mohler P, Gramolini A, Bennett V. The ankyrin-B C-terminal domain determines activity of ankyrin-B/G chimeras in rescue of abnormal inositol 1,4,5-trisphosphate and ryanodine receptor distribution in ankyrin-B (-/-) neonatal cardiomyocytes. J Biol Chem. 2002;277:10599-607 pubmed..C-terminal domains are the most divergent between ankyrin isoforms and are candidates to encode the signal(s) that enable ankyrins to selectively target proteins to diverse cellular sites. ..
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