Genomes and Genes
Experts and Doctors on protein binding in Durham, North Carolina, United States
Locale: Durham, North Carolina, United States
Topic: protein binding
Publications185 found, 100 shown here
- Li X, Bennett V. Identification of the spectrin subunit and domains required for formation of spectrin/adducin/actin complexes. J Biol Chem. 1996;271:15695-702 pubmed
- Naga Prasad S, Barak L, Rapacciuolo A, Caron M, Rockman H. Agonist-dependent recruitment of phosphoinositide 3-kinase to the membrane by beta-adrenergic receptor kinase 1. A role in receptor sequestration. J Biol Chem. 2001;276:18953-9 pubmed
- Crawford J, Su Z, Varlamova O, Bresnick A, Kiehart D. Role of myosin-II phosphorylation in V12Cdc42-mediated disruption of Drosophila cellularization. Eur J Cell Biol. 2001;80:240-4 pubmed..These findings suggest that increased levels of RLC phosphorylation do not contribute to disruption of the actomyosin hexagonal array. ..
- Breazeale S, Ribeiro A, Raetz C. Oxidative decarboxylation of UDP-glucuronic acid in extracts of polymyxin-resistant Escherichia coli. Origin of lipid a species modified with 4-amino-4-deoxy-L-arabinose. J Biol Chem. 2002;277:2886-96 pubmed..ArnA resembles UDP-galactose epimerase, dTDP-glucose-4,6-dehydratase, and UDP-xylose synthase in oxidizing the C-4" position of its substrate, but differs in that it releases the NADH product. ..
- Kreider M, Levin E, Seidler F, Slotkin T. Gestational dexamethasone treatment elicits sex-dependent alterations in locomotor activity, reward-based memory and hippocampal cholinergic function in adolescent and adult rats. Neuropsychopharmacology. 2005;30:1617-23 pubmed
- Hallen M, Ho J, Yankel C, Endow S. Fluorescence recovery kinetic analysis of gamma-tubulin binding to the mitotic spindle. Biophys J. 2008;95:3048-58 pubmed publisher..gamma-Tubulin may interact transiently with the spindle, nucleating microtubules very rapidly, differing from centrosomes, where gamma-tubulin binds tightly to nucleate microtubules. ..
- Dobrikova E, Shveygert M, Walters R, Gromeier M. Herpes simplex virus proteins ICP27 and UL47 associate with polyadenylate-binding protein and control its subcellular distribution. J Virol. 2010;84:270-9 pubmed publisher..This indicates that redistribution of PABPC1 may be involved in co- and posttranscriptional regulation of mRNA processing and/or nuclear export by HSV-1 gene regulatory proteins...
- Mina J, Mosely J, Ali H, Denny P, Steel P. Exploring Leishmania major inositol phosphorylceramide synthase (LmjIPCS): insights into the ceramide binding domain. Org Biomol Chem. 2011;9:1823-30 pubmed publisher..Whilst the N-acyl chain also contributed to affinity, analogues lacking the amide linkage functioned as competitive inhibitors in both enzyme and cell-based assays. A model that accounts for this observation is proposed. ..
- Dobrikov M, Dobrikova E, Gromeier M. Dynamic regulation of the translation initiation helicase complex by mitogenic signal transduction to eukaryotic translation initiation factor 4G. Mol Cell Biol. 2013;33:937-46 pubmed publisher..Our results provide a mechanistic link between intracellular signal transduction and dynamic initiation complex formation coordinated by flexible eIF4G structure. ..
- Patil H, Cho K, Lee J, Yang Y, Orry A, Ferreira P. Kinesin-1 and mitochondrial motility control by discrimination of structurally equivalent but distinct subdomains in Ran-GTP-binding domains of Ran-binding protein 2. Open Biol. 2013;3:120183 pubmed publisher..These findings open new venues towards the pharmacological harnessing of cooperative and competitive mechanisms regulating kinesins, RanBP2 or mitochondrial motility in disparate human disorders. ..
- Gonzalez Gronow M, Fiedler J, Farias Gomez C, Wang F, Ray R, Ferrell P, et al. Myelin basic protein stimulates plasminogen activation via tissue plasminogen activator following binding to independent l-lysine-containing domains. Biochem Biophys Res Commun. 2017;490:855-860 pubmed publisher..In this context, MBP mimics fibrin and because MBP is a plasmin substrate, our results suggest direct participation of the Pg activation system on MBP physiology. ..
- Chervenak M, Toone E. Calorimetric analysis of the binding of lectins with overlapping carbohydrate-binding ligand specificities. Biochemistry. 1995;34:5685-95 pubmed..Our studies clearly demonstrate that, despite their common affinity for the mannose trisaccharide, the three lectins bind oligosaccharides very differently. ..
- Bussiere D, Bastia D, White S. Crystal structure of the replication terminator protein from B. subtilis at 2.6 A. Cell. 1995;80:651-60 pubmed..Other features of the structure are consistent with the polar contrahelicase mechanism of the protein. A model of the interaction between RTP and its cognate DNA is presented. ..
- Song H, Golovkin M, Reddy A, Endow S. In vitro motility of AtKCBP, a calmodulin-binding kinesin protein of Arabidopsis. Proc Natl Acad Sci U S A. 1997;94:322-7 pubmed
- Tedder T, Inaoki M, Sato S. The CD19-CD21 complex regulates signal transduction thresholds governing humoral immunity and autoimmunity. Immunity. 1997;6:107-18 pubmed
- Lin F, Krueger K, Kendall H, Daaka Y, Fredericks Z, Pitcher J, et al. Clathrin-mediated endocytosis of the beta-adrenergic receptor is regulated by phosphorylation/dephosphorylation of beta-arrestin1. J Biol Chem. 1997;272:31051-7 pubmed..Thus, as with the classical endocytic adaptor protein complex AP2, beta-arrestin1 functions as a clathrin adaptor in receptor endocytosis which is regulated by dephosphorylation at the plasma membrane. ..
- Hall R, Premont R, Chow C, Blitzer J, Pitcher J, Claing A, et al. The beta2-adrenergic receptor interacts with the Na+/H+-exchanger regulatory factor to control Na+/H+ exchange. Nature. 1998;392:626-30 pubmed..Our findings indicate that agonist-dependent beta2-adrenergic receptor binding of NHERF plays a role in beta2-adrenergic receptor-mediated regulation of Na+/H+ exchange. ..
- LaJeunesse D, McCartney B, Fehon R. A systematic screen for dominant second-site modifiers of Merlin/NF2 phenotypes reveals an interaction with blistered/DSRF and scribbler. Genetics. 2001;158:667-79 pubmed..These results suggest that Merlin, blistered, and scribbler function together in a common pathway to regulate Drosophila wing epithelial development. ..
- Blackwell L, Bjornson K, Allen D, Modrich P. Distinct MutS DNA-binding modes that are differentially modulated by ATP binding and hydrolysis. J Biol Chem. 2001;276:34339-47 pubmed..Conversely, the highest mismatch specificity is observed in the absence of nucleotide or in the presence of ADP. ADP has only a limited effect on heteroduplex affinity but reduces MutS affinity for homoduplex DNA. ..
- Wiegand H, Coburn G, Zeng Y, Kang Y, Bogerd H, Cullen B. Formation of Tap/NXT1 heterodimers activates Tap-dependent nuclear mRNA export by enhancing recruitment to nuclear pore complexes. Mol Cell Biol. 2002;22:245-56 pubmed..These data suggest that NXT1 may act as a molecular switch that regulates the ability of Tap to mediate nuclear mRNA export by controlling the interaction of Tap with components of the nuclear pore. ..
- Bloor J, Kiehart D. zipper Nonmuscle myosin-II functions downstream of PS2 integrin in Drosophila myogenesis and is necessary for myofibril formation. Dev Biol. 2001;239:215-28 pubmed publisher..We suggest that nonmuscle myosin-II functions at the muscle termini and the Z-line as an actin crosslinker and acts to maintain the structural integrity of the sarcomere...
- Shaffer P, Gewirth D. Structural basis of VDR-DNA interactions on direct repeat response elements. EMBO J. 2002;21:2242-52 pubmed..Selective structure-based mutations in the asymmetric homodimeric interface result in a VDR DBD protein that is defective in homodimerization but now forms heterodimers with the 9-cis retinoic acid receptor (RXR) DBD. ..
- Gonzalez Gronow M, Gawdi G, Pizzo S. Tissue factor is the receptor for plasminogen type 1 on 1-LN human prostate cancer cells. Blood. 2002;99:4562-7 pubmed..The current studies suggest that Pg oligosaccharide chains regulate the binding of Pg 1 and Pg 2 to separate receptors on the cell surface. ..
- Painter G, Almond M, Mao S, Liotta D. Biochemical and mechanistic basis for the activity of nucleoside analogue inhibitors of HIV reverse transcriptase. Curr Top Med Chem. 2004;4:1035-44 pubmed..The results provide insight into the molecular forces and structural features that make these molecules effective inhibitors. ..
- Premont R, Perry S, Schmalzigaug R, Roseman J, Xing Y, Claing A. The GIT/PIX complex: an oligomeric assembly of GIT family ARF GTPase-activating proteins and PIX family Rac1/Cdc42 guanine nucleotide exchange factors. Cell Signal. 2004;16:1001-11 pubmed..Thus, GIT and PIX proteins are tightly associated as a multimeric nexus capable of linking together important signaling molecules, including PAKs. ..
- Shenoy S, Lefkowitz R. Receptor-specific ubiquitination of beta-arrestin directs assembly and targeting of seven-transmembrane receptor signalosomes. J Biol Chem. 2005;280:15315-24 pubmed
- Zhang X, Yang F, Shim J, Kirk K, Anderson D, Chen X. Identification of arsenic-binding proteins in human breast cancer cells. Cancer Lett. 2007;255:95-106 pubmed..Functional consequence of such binding may depend on whether arsenic binding causes conformational changes or blocks active sites of target proteins. ..
- Zou J, Hallen M, Yankel C, Endow S. A microtubule-destabilizing kinesin motor regulates spindle length and anchoring in oocytes. J Cell Biol. 2008;180:459-66 pubmed publisher..By altering microtubule dynamics, KLP10A could promote spindle reorientation upon oocyte activation. ..
- Zhang J, Guo H, Mi Z, Gao C, Bhattacharya S, Li J, et al. EF1A1-actin interactions alter mRNA stability to determine differential osteopontin expression in HepG2 and Hep3B cells. Exp Cell Res. 2009;315:304-12 pubmed publisher..The converse findings were noted in HepG2 cells. Overall, our results suggest that EF1A1 regulation of OPN mRNA stability is actin dependent. EF1A1 has not been previously identified as a regulatory factor in OPN expression in cancer. ..
- Tilley D, Kim I, Patel P, Violin J, Rockman H. beta-Arrestin mediates beta1-adrenergic receptor-epidermal growth factor receptor interaction and downstream signaling. J Biol Chem. 2009;284:20375-86 pubmed publisher..These data reveal a new signaling paradigm in which beta-arrestin is required for the maintenance of a beta1AR-EGFR interaction that can direct cytosolic targeting of ERK in response to catecholamine stimulation. ..
- Hamburger J, Hoertz A, Lee A, Senturia R, McCafferty D, Loll P. A crystal structure of a dimer of the antibiotic ramoplanin illustrates membrane positioning and a potential Lipid II docking interface. Proc Natl Acad Sci U S A. 2009;106:13759-64 pubmed publisher..The structure also suggests a mechanism by which ramoplanin A2 recognizes its Lipid II ligand. ..
- Yang Y, Calakos N. Munc13-1 is required for presynaptic long-term potentiation. J Neurosci. 2011;31:12053-7 pubmed publisher..These results advance our understanding of the molecular mechanisms of presynaptic plasticity and suggest that modulation of vesicle priming may provide the cellular substrate for expression of LTP at mossy fiber synapses. ..
- Alam S, Liao H, Dennison S, Jaeger F, Parks R, Anasti K, et al. Differential reactivity of germ line allelic variants of a broadly neutralizing HIV-1 antibody to a gp41 fusion intermediate conformation. J Virol. 2011;85:11725-31 pubmed publisher..Thus, these data demonstrate a genetically determined trait that may affect host responses to HIV-1 envelope epitopes recognized by broadly neutralizing antibodies and has implications for unmutated ancestor-based immunogen design. ..
- He M, Tseng W, Bennett V. A single divergent exon inhibits ankyrin-B association with the plasma membrane. J Biol Chem. 2013;288:14769-79 pubmed publisher..These results provide a simple evolutionary explanation for how ankyrin-B and ankyrin-G have acquired striking differences in their plasma membrane association while maintaining overall high levels of sequence similarity...
- Colbert M, Rubin W, Linney E, LaMantia A. Retinoid signaling and the generation of regional and cellular diversity in the embryonic mouse spinal cord. Dev Dyn. 1995;204:1-12 pubmed
- Wharton R, Sonoda J, Lee T, Patterson M, Murata Y. The Pumilio RNA-binding domain is also a translational regulator. Mol Cell. 1998;1:863-72 pubmed..We also show that Nos and Pum can repress cap-independent translation from an internal ribosome entry site (IRES) in vivo, suggesting that they act downstream of the initial steps of normal, cap-dependent translation. ..
- Herold A, Truant R, Wiegand H, Cullen B. Determination of the functional domain organization of the importin alpha nuclear import factor. J Cell Biol. 1998;143:309-18 pubmed..Importantly, the SV-40 T NLS proved able to specifically inhibit the interaction of Imp alpha with CAS in vitro, thus explaining why the SV-40 T NLS is unable to also function as a nuclear export signal. ..
- Tang Y, Hu L, Miller W, Ringstad N, Hall R, Pitcher J, et al. Identification of the endophilins (SH3p4/p8/p13) as novel binding partners for the beta1-adrenergic receptor. Proc Natl Acad Sci U S A. 1999;96:12559-64 pubmed
- Richard E, Arredondo Vega F, Santisteban I, Kelly S, Patel D, Hershfield M. The binding site of human adenosine deaminase for CD26/Dipeptidyl peptidase IV: the Arg142Gln mutation impairs binding to cd26 but does not cause immune deficiency. J Exp Med. 2000;192:1223-36 pubmed..Conversely, ADA with the E217K active site mutation, the only ADA expressed by a severely immunodeficient patient, showed normal CD26 binding. These findings argue that ADA binding to CD26 is not essential for immune function in humans. ..
- Blobe G, Liu X, Fang S, How T, Lodish H. A novel mechanism for regulating transforming growth factor beta (TGF-beta) signaling. Functional modulation of type III TGF-beta receptor expression through interaction with the PDZ domain protein, GIPC. J Biol Chem. 2001;276:39608-17 pubmed..These results further support an essential, non-redundant role for the type III receptor in TGF-beta signaling. ..
- Harashima T, Heitman J. The Galpha protein Gpa2 controls yeast differentiation by interacting with kelch repeat proteins that mimic Gbeta subunits. Mol Cell. 2002;10:163-73 pubmed..Our studies demonstrate that Gpa2 signals in conjunction with Gbeta structural mimics and that homologous G protein subunits or effectors may be conserved in multicellular eukaryotes. ..
- Martin N, Lefkowitz R, Shenoy S. Regulation of V2 vasopressin receptor degradation by agonist-promoted ubiquitination. J Biol Chem. 2003;278:45954-9 pubmed..However, receptor stimulation leads to rapid beta-arrestin2-dependent ubiquitination of the receptor and increased degradation. ..
- Cook J, Chasse D, Nevins J. The regulated association of Cdt1 with minichromosome maintenance proteins and Cdc6 in mammalian cells. J Biol Chem. 2004;279:9625-33 pubmed
- Jones W, Bejsovec A. RacGap50C negatively regulates wingless pathway activity during Drosophila embryonic development. Genetics. 2005;169:2075-86 pubmed..These phenotypes suggest that the RacGap50C gene product participates in the negative regulation of Wg pathway activity. ..
- Casaletto J, Nutt L, Wu Q, Moore J, Etkin L, Jackson P, et al. Inhibition of the anaphase-promoting complex by the Xnf7 ubiquitin ligase. J Cell Biol. 2005;169:61-71 pubmed..Furthermore, Xnf7 was required for spindle assembly checkpoint function in egg extracts. These data suggest that Xnf7 is an APC inhibitor able to link spindle status to the APC through direct association with APC core components. ..
- Chen Y, Erickson H. Rapid in vitro assembly dynamics and subunit turnover of FtsZ demonstrated by fluorescence resonance energy transfer. J Biol Chem. 2005;280:22549-54 pubmed..The mechanism may be related to the dynamic instability of microtubules. ..
- Levesque M, Vernoux T, Busch W, Cui H, Wang J, Blilou I, et al. Whole-genome analysis of the SHORT-ROOT developmental pathway in Arabidopsis. PLoS Biol. 2006;4:e143 pubmed..Taken together, our results not only identify the first nodes in the SHR pathway and a new function for SHR in the development of the vascular tissue but also reveal the global architecture of this developmental pathway. ..
- Meloni A, Fralish G, Kelly P, Salahpour A, Chen J, Wechsler Reya R, et al. Smoothened signal transduction is promoted by G protein-coupled receptor kinase 2. Mol Cell Biol. 2006;26:7550-60 pubmed..Together, these data suggest that GRK2 plays a positive role in Smoothened signaling, at least in part, through the promotion of an association between beta-arrestin 2 and Smoothened. ..
- Cui H, Levesque M, Vernoux T, Jung J, Paquette A, Gallagher K, et al. An evolutionarily conserved mechanism delimiting SHR movement defines a single layer of endodermis in plants. Science. 2007;316:421-5 pubmed..Our studies with SHR and SCR homologs from rice suggest that this mechanism is evolutionarily conserved, providing a plausible explanation why nearly all plants have a single layer of endodermis. ..
- Xiao K, McClatchy D, Shukla A, Zhao Y, Chen M, Shenoy S, et al. Functional specialization of beta-arrestin interactions revealed by proteomic analysis. Proc Natl Acad Sci U S A. 2007;104:12011-6 pubmed..This study provides a comprehensive analysis of proteins that bind beta-arrestin isoforms and underscores their potentially broad regulatory roles in mammalian cellular physiology. ..
- Barrientos K, Kendellen M, Freibaum B, Armbruster B, Etheridge K, Counter C. Distinct functions of POT1 at telomeres. Mol Cell Biol. 2008;28:5251-64 pubmed publisher..Mechanistically, binding of POT1 to telomeric ssDNA and association with TPP1 inhibit the localization of RPA, which can function as a DNA damage sensor, to telomeres. ..
- Slotkin T, Lassiter T, Ryde I, Wrench N, Levin E, Seidler F. Consumption of a high-fat diet in adulthood ameliorates the effects of neonatal parathion exposure on acetylcholine systems in rat brain regions. Environ Health Perspect. 2009;117:916-22 pubmed publisher..Further, cryptic neurodevelopmental deficits evoked by environmental exposures may thus engender a later preference for a high-fat diet to maintain normal ACh function, ultimately contributing to obesity. ..
- Gordân R, Hartemink A, Bulyk M. Distinguishing direct versus indirect transcription factor-DNA interactions. Genome Res. 2009;19:2090-100 pubmed publisher..Our method is not restricted to yeast or to ChIP-chip data, but can be applied in any system for which both in vivo binding data and in vitro DNA binding motifs are available. ..
- Evron T, Philipp M, Lu J, Meloni A, Burkhalter M, Chen W, et al. Growth Arrest Specific 8 (Gas8) and G protein-coupled receptor kinase 2 (GRK2) cooperate in the control of Smoothened signaling. J Biol Chem. 2011;286:27676-86 pubmed publisher..This is in contrast to cells stably expressing both GRK2 and Smo, in which cilia are significantly elongated. These results identify Gas8 as a positive regulator of Hh signaling that cooperates with GRK2 to control Smo signaling. ..
- Liu H, Hallen M, Endow S. Altered nucleotide-microtubule coupling and increased mechanical output by a kinesin mutant. PLoS ONE. 2012;7:e47148 pubmed publisher..One possibility, based on our findings, is that movements by the residue and the loop that contains it could bend or distort the central ?-sheet, mediating free energy changes that lead to force production. ..
- Leung J, Nevins J. E2F6 associates with BRG1 in transcriptional regulation. PLoS ONE. 2012;7:e47967 pubmed publisher..Collectively, our studies suggest that E2F6 may recruit BRG1 in transcriptional regulation of genes important for G1/S phase transition of the cell cycle. ..
- Maddala R, Walters M, Brophy P, Bennett V, Rao P. Ankyrin-B directs membrane tethering of periaxin and is required for maintenance of lens fiber cell hexagonal shape and mechanics. Am J Physiol Cell Physiol. 2016;310:C115-26 pubmed publisher..Taken together, these observations reveal that AnkB is required for Prx membrane anchoring and for maintenance of lens fiber cell hexagonal geometry, membrane skeleton organization, and biomechanics. ..
- Monroe R, Ostrowski J, Hryniewicz M, Kredich N. In vitro interactions of CysB protein with the cysK and cysJIH promoter regions of Salmonella typhimurium. J Bacteriol. 1990;172:6919-29 pubmed..CBS-J is analogous in position to CBS-K1 and is probably also required for activity of the cysJIH promoter. CBS-K2 has no known function but may help sequester CysB protein at the cysK promoter. ..
- Fargin A, Raymond J, Lohse M, Kobilka B, Caron M, Lefkowitz R. The genomic clone G-21 which resembles a beta-adrenergic receptor sequence encodes the 5-HT1A receptor. Nature. 1988;335:358-60 pubmed..We now report that the protein product of the genomic clone, G21, transiently expressed in monkey kidney cells has all the typical ligand-binding characteristics of the 5-hydroxytryptamine (5-HT1A) receptor. ..
- Telen M, Udani M, Washington M, Levesque M, Lloyd E, Rao N. A blood group-related polymorphism of CD44 abolishes a hyaluronan-binding consensus sequence without preventing hyaluronan binding. J Biol Chem. 1996;271:7147-53 pubmed..Competitive inhibition studies using human anti-Inb also showed that some mAbs that inhibit HA binding to CD44 may do so by interacting with a domain separate from, but affecting the structure of, the Inb epitope. ..
- Bogerd H, Echarri A, Ross T, Cullen B. Inhibition of human immunodeficiency virus Rev and human T-cell leukemia virus Rex function, but not Mason-Pfizer monkey virus constitutive transport element activity, by a mutant human nucleoporin targeted to Crm1. J Virol. 1998;72:8627-35 pubmed..In addition, these data identify a novel and highly potent inhibitor of leucine-rich NES-dependent nuclear export...
- Fujimoto M, Poe J, Jansen P, Sato S, Tedder T. CD19 amplifies B lymphocyte signal transduction by regulating Src-family protein tyrosine kinase activation. J Immunol. 1999;162:7088-94 pubmed..The absence of this CD19/Src-family kinase amplification loop may account for the hyporesponsive phenotype of CD19-deficient B cells. ..
- Oakley R, Laporte S, Holt J, Barak L, Caron M. Molecular determinants underlying the formation of stable intracellular G protein-coupled receptor-beta-arrestin complexes after receptor endocytosis*. J Biol Chem. 2001;276:19452-60 pubmed
- Yang J, Bogerd H, Wang P, Page D, Cullen B. Two closely related human nuclear export factors utilize entirely distinct export pathways. Mol Cell. 2001;8:397-406 pubmed..Thus, while the related TAP and NXF3 proteins are both capable of mediating nuclear RNA export, they do so via unrelated export pathways...
- Zawistowski J, Serebriiskii I, Lee M, Golemis E, Marchuk D. KRIT1 association with the integrin-binding protein ICAP-1: a new direction in the elucidation of cerebral cavernous malformations (CCM1) pathogenesis. Hum Mol Genet. 2002;11:389-96 pubmed..Furthermore, these data suggest that KRIT1 might affect cell adhesion processes via integrin signaling in CCM1 pathogenesis. ..
- Freedman N, Kim L, Murray J, Exum S, Brian L, Wu J, et al. Phosphorylation of the platelet-derived growth factor receptor-beta and epidermal growth factor receptor by G protein-coupled receptor kinase-2. Mechanisms for selectivity of desensitization. J Biol Chem. 2002;277:48261-9 pubmed..Specificity of GRK2 for receptor protein-tyrosine kinases, expressed at physiologic levels, may be determined by the ability of these receptors to activate heterotrimeric G proteins, among other factors. ..
- Hu L, Chen W, Martin N, Whalen E, Premont R, Lefkowitz R. GIPC interacts with the beta1-adrenergic receptor and regulates beta1-adrenergic receptor-mediated ERK activation. J Biol Chem. 2003;278:26295-301 pubmed..These data suggest that GIPC can regulate beta1-adrenergic receptor-stimulated, Gi-mediated, ERK activation while having no effect on receptor internalization or Gs-mediated cAMP signaling. ..
- Mathew S, Arandjelovic S, Beyer W, Gonias S, Pizzo S. Characterization of the interaction between alpha2-macroglobulin and fibroblast growth factor-2: the role of hydrophobic interactions. Biochem J. 2003;374:123-9 pubmed..Our results demonstrate that the interactions of FGF-2 with alpha2M* and HSPGs are biochemically distinct, suggesting that different FGF-2 sequences are involved. ..
- Alcorn J, Wright J. Degradation of pulmonary surfactant protein D by Pseudomonas aeruginosa elastase abrogates innate immune function. J Biol Chem. 2004;279:30871-9 pubmed..These data show that degradation of SP-D occurs in the BAL environment and that degradation eliminates many normal immune functions of SP-D. ..
- Allende D, Simon S, McIntosh T. Melittin-induced bilayer leakage depends on lipid material properties: evidence for toroidal pores. Biophys J. 2005;88:1828-37 pubmed..All of these results demonstrate the importance of bilayer material properties on melittin-induced leakage and indicate that the melittin-induced pores are defects in the bilayer lined in part by lipid molecules. ..
- Irazoqui J, Howell A, Theesfeld C, Lew D. Opposing roles for actin in Cdc42p polarization. Mol Biol Cell. 2005;16:1296-304 pubmed..In addition, we report that once a bud has formed, polarized Cdc42p becomes more resistant to dispersal, revealing an unexpected difference between unbudded and budded cells in the organization of the polarization site. ..
- Beaulieu J, Sotnikova T, Marion S, Lefkowitz R, Gainetdinov R, Caron M. An Akt/beta-arrestin 2/PP2A signaling complex mediates dopaminergic neurotransmission and behavior. Cell. 2005;122:261-73 pubmed
- Hallstrom T, Nevins J. Jab1 is a specificity factor for E2F1-induced apoptosis. Genes Dev. 2006;20:613-23 pubmed..Cells depleted of Jab1 are deficient for both E2F1-induced apoptosis and induction of p53 accumulation. We suggest that Jab1 is an essential cofactor for the apoptotic function of E2F1. ..
- Williams A, Immormino R, Gewirth D, Raetz C. Structure of UDP-N-acetylglucosamine acyltransferase with a bound antibacterial pentadecapeptide. Proc Natl Acad Sci U S A. 2006;103:10877-82 pubmed..The compact beta-turn structure of peptide 920 bound to LpxA may open previously uncharacterized approaches to the rational design of LpxA inhibitors with antibiotic activity. ..
- Hoffman M, Colina C, McDonald A, Arepally G, Pedersen L, Monroe D. Tissue factor around dermal vessels has bound factor VII in the absence of injury. J Thromb Haemost. 2007;5:1403-8 pubmed..The presence of endogenously bound FVIIa prevents the subsequent binding of the monospecific antibody or exogenous FVIIai to perivascular TF. ..
- Lee N, Blobe G. The interaction of endoglin with beta-arrestin2 regulates transforming growth factor-beta-mediated ERK activation and migration in endothelial cells. J Biol Chem. 2007;282:21507-17 pubmed..These studies define a novel function for endoglin, and further expand the roles mediated by the ubiquitous scaffolding protein beta-arrestin2. ..
- Bolger T, Zhao X, Cohen T, Tsai C, Yao T. The neurodegenerative disease protein ataxin-1 antagonizes the neuronal survival function of myocyte enhancer factor-2. J Biol Chem. 2007;282:29186-92 pubmed..Our results identify the MEF2-HDAC4 complex as a target for ataxin-1 transcriptional repression activity and suggest a novel pathogenic mechanism whereby ataxin-1 sequesters and inhibits the neuronal survival factor MEF2. ..
- Noma T, Lemaire A, Naga Prasad S, Barki Harrington L, Tilley D, Chen J, et al. Beta-arrestin-mediated beta1-adrenergic receptor transactivation of the EGFR confers cardioprotection. J Clin Invest. 2007;117:2445-58 pubmed
- Gonzalez Gronow M, Kaczowka S, Payne S, Wang F, Gawdi G, Pizzo S. Plasminogen structural domains exhibit different functions when associated with cell surface GRP78 or the voltage-dependent anion channel. J Biol Chem. 2007;282:32811-20 pubmed..We show that kringle 5 inhibits 1-LN cell proliferation and promotes caspase-7 activity by a mechanism that requires binding to cell surface voltage-dependent anion channel and is inhibited by human hexokinase I. ..
- Abdi K, Bennett V. Adducin promotes micrometer-scale organization of beta2-spectrin in lateral membranes of bronchial epithelial cells. Mol Biol Cell. 2008;19:536-45 pubmed..We conclude that adducin acting through spectrin provides a novel mechanism to regulate global properties of the lateral membrane of bronchial epithelial cells. ..
- Shim J, Bertalovitz A, Kendall D. Identification of essential cannabinoid-binding domains: structural insights into early dynamic events in receptor activation. J Biol Chem. 2011;286:33422-35 pubmed publisher..The functional data for the mutant receptors demonstrated reductions in potency for G protein activation similar to the reductions seen in ligand binding affinity for HU210. ..
- Choi M, Cohen T, Barrientos T, Wang B, Li M, Simmons B, et al. A direct HDAC4-MAP kinase crosstalk activates muscle atrophy program. Mol Cell. 2012;47:122-32 pubmed publisher..Our findings establish an HDAC4-MAPK-AP1 signaling axis essential for neurogenic muscle atrophy and uncover a direct crosstalk between acetylation- and phosphorylation-dependent signaling cascades. ..
- ..We speculate that the N-terminal region of Snm1B forms a complex containing PSF2 and Mus81, while the C-terminal region is important for PSF2-mediated chromatin association...
- Sheffield N, Thurman R, Song L, Safi A, Stamatoyannopoulos J, Lenhard B, et al. Patterns of regulatory activity across diverse human cell types predict tissue identity, transcription factor binding, and long-range interactions. Genome Res. 2013;23:777-88 pubmed publisher..Finally, we introduce a web resource to enable researchers to use these results to explore these regulatory patterns and better understand how expression is modulated within and across human cell types. ..
- Smiley R, Zhuang Z, Benkovic S, Hammes G. Single-molecule investigation of the T4 bacteriophage DNA polymerase holoenzyme: multiple pathways of holoenzyme formation. Biochemistry. 2006;45:7990-7 pubmed publisher..In all cases, MgATP is required. The possible physiological significance of the various assembly pathways is discussed in the context of replication initiation and lagging strand synthesis during various stages of T4 phage replication...
- Mohler P, Rivolta I, Napolitano C, LeMaillet G, Lambert S, Priori S, et al. Nav1.5 E1053K mutation causing Brugada syndrome blocks binding to ankyrin-G and expression of Nav1.5 on the surface of cardiomyocytes. Proc Natl Acad Sci U S A. 2004;101:17533-8 pubmed..Together with previous work in neurons, these results in cardiomyocytes suggest that ankyrin-G participates in a common pathway for localization of voltage-gated Na(v) channels at sites of function in multiple excitable cell types. ..
- Jones M, Kondo M, Zhuang Y. A tamoxifen inducible knock-in allele for investigation of E2A function. BMC Dev Biol. 2009;9:51 pubmed publisher..These features make our E2AER ex vivo culture system attractive to study both immediate and gradual downstream E2A-mediated events. ..
- Hong L, Simon J. Insights into the thermodynamics of copper association with amyloid-?, ?-synuclein and prion proteins. Metallomics. 2011;3:262-6 pubmed publisher..This current review reconciles the disparate models presented in the literature. ..
- Urban J, Merten C. Retroviral display in gene therapy, protein engineering, and vaccine development. ACS Chem Biol. 2011;6:61-74 pubmed publisher..This review critically summarizes the basic concepts and applications of retroviral display and analyses its benefits in comparison to other display techniques. ..
- Koonpaew S, Janssen E, Zhu M, Zhang W. The importance of three membrane-distal tyrosines in the adaptor protein NTAL/LAB. J Biol Chem. 2004;279:11229-35 pubmed..Mutation of these three tyrosines abolished Grb2 binding and LAB function. Our data suggested that these tyrosines are the most important tyrosines for LAB function. ..