Genomes and Genes
Experts and Doctors on nuclear proteins in Chapel Hill, North Carolina, United States
Locale: Chapel Hill, North Carolina, United States
Topic: nuclear proteins
Publications185 found, 100 shown here
- Coleman K, Grant G, Haggerty R, Brantley K, Shibata E, Workman B, et al. Sequential replication-coupled destruction at G1/S ensures genome stability. Genes Dev. 2015;29:1734-46 pubmed publisher..We therefore propose that sequential degradation ensures orderly S-phase progression to avoid replication stress and genome instability. ..
- Yeh E, Yang C, Chin E, Maddox P, Salmon E, Lew D, et al. Dynamic positioning of mitotic spindles in yeast: role of microtubule motors and cortical determinants. Mol Biol Cell. 2000;11:3949-61 pubmed..Cytoplasmic dynein is attenuated by these spindle polarity determinants and kinesin until anaphase onset, when dynein directs spindle elongation to distal points in the mother and bud. ..
- Bassi G, de Oliveira D, White M, Weeks K. Recruitment of intron-encoded and co-opted proteins in splicing of the bI3 group I intron RNA. Proc Natl Acad Sci U S A. 2002;99:128-33 pubmed..Thus, the bI3 ribonucleoprotein is the product of a process in which a once-catalytically active RNA now obligatorily requires two facilitating protein cofactors, both of which are compromised in their original functions. ..
- Mahajan K, Nick McElhinny S, Mitchell B, Ramsden D. Association of DNA polymerase mu (pol mu) with Ku and ligase IV: role for pol mu in end-joining double-strand break repair. Mol Cell Biol. 2002;22:5194-202 pubmed..Our data thus support an important role for pol mu in the end-joining pathway for repair of double-strand breaks. ..
- Wong A, Ghosh N, McKinnon K, Reed W, Piskurich J, Wright K, et al. Regulation and specificity of MHC2TA promoter usage in human primary T lymphocytes and cell line. J Immunol. 2002;169:3112-9 pubmed..These results show that MHC2TA PIII is induced in activated T lymphocytes, and that the induced binding of ARE-2 is a crucial step in this process. ..
- DeLuca J, Moree B, Hickey J, Kilmartin J, Salmon E. hNuf2 inhibition blocks stable kinetochore-microtubule attachment and induces mitotic cell death in HeLa cells. J Cell Biol. 2002;159:549-55 pubmed
- Alekseev O, Richardson R, TSURUTA J, O Rand M. Depletion of the histone chaperone tNASP inhibits proliferation and induces apoptosis in prostate cancer PC-3 cells. Reprod Biol Endocrinol. 2011;9:50 pubmed publisher..This study has demonstrated that tNASP is critical for the survival of prostate cancer cells and suggests that targeting tNASP expression can lead to a new approach for prostate cancer treatment. ..
- Compton S, Choi J, Cesare A, Ozgur S, Griffith J. Xrcc3 and Nbs1 are required for the production of extrachromosomal telomeric circles in human alternative lengthening of telomere cells. Cancer Res. 2007;67:1513-9 pubmed..We show that Xrcc3 and Nbs1 are indeed required for the production of t circles in human ALT. However, these cells continue to proliferate in the absence of t circles, suggesting that they are not required for the survival of ALT cells. ..
- Koseoglu M, Graves L, Marzluff W. Phosphorylation of threonine 61 by cyclin a/Cdk1 triggers degradation of stem-loop binding protein at the end of S phase. Mol Cell Biol. 2008;28:4469-79 pubmed publisher..We conclude that the increase in cyclin A/Cdk1 activity at the end of S phase triggers degradation of SLBP at S/G(2). ..
- Passman J, Dong X, Wu S, Maguire C, Hogan K, Bautch V, et al. A sonic hedgehog signaling domain in the arterial adventitia supports resident Sca1+ smooth muscle progenitor cells. Proc Natl Acad Sci U S A. 2008;105:9349-54 pubmed publisher..We conclude that a restricted domain of Shh signaling is localized to the arterial adventitia and may play important roles in maintenance of resident vascular SMC progenitor cells in the artery wall. ..
- Lee Y, O Rand M. Ultrastructural localization of a nuclear autoantigenic sperm protein in spermatogenic cells and spermatozoa. Anat Rec. 1993;236:442-8 pubmed..It is likely that NASP would be carried into the ovum with the sperm nucleus at fertilization. ..
- Hardman R, Afshari C, Barrett J. Involvement of mammalian MLH1 in the apoptotic response to peroxide-induced oxidative stress. Cancer Res. 2001;61:1392-7 pubmed..Together, our data indicate that cells lacking MLH1 may possess a selective growth advantage under oxidatively stressed conditions via the disregulation of apoptosis, possibly involving the mitochondria. ..
- Dean K, von Ahsen O, Gorlich D, Fried H. Signal recognition particle protein 19 is imported into the nucleus by importin 8 (RanBP8) and transportin. J Cell Sci. 2001;114:3479-85 pubmed..Our results show that at least one mammalian SRP protein is specifically imported into the nucleus, by members of the importin beta family of transport receptors, and the findings add additional evidence for nuclear assembly of SRP. ..
- He B, Gampe R, Kole A, Hnat A, Stanley T, An G, et al. Structural basis for androgen receptor interdomain and coactivator interactions suggests a transition in nuclear receptor activation function dominance. Mol Cell. 2004;16:425-38 pubmed
- Ning S, Huye L, Pagano J. Regulation of the transcriptional activity of the IRF7 promoter by a pathway independent of interferon signaling. J Biol Chem. 2005;280:12262-70 pubmed..A regulatory network between type I IFNs and IRF7 is proposed. The distinct pathways may reflect special roles for an efficient antiviral response at different stages of virus infection. ..
- Jang K, Shackelford J, Seo S, Pagano J. Up-regulation of beta-catenin by a viral oncogene correlates with inhibition of the seven in absentia homolog 1 in B lymphoma cells. Proc Natl Acad Sci U S A. 2005;102:18431-6 pubmed publisher..Thus, we demonstrate the significance of the endogenous Siah-1-dependent ubiquitin/proteasome pathway for beta-catenin degradation in malignant human cells and its regulation by a viral oncogene...
- Bergstralh D, Conti B, Moore C, Brickey W, Taxman D, Ting J. Global functional analysis of nucleophosmin in Taxol response, cancer, chromatin regulation, and ribosomal DNA transcription. Exp Cell Res. 2007;313:65-76 pubmed..These results demonstrate multi-faceted functions of NPM that can affect cancer cells. ..
- Larocque J, Jaklevic B, Su T, Sekelsky J. Drosophila ATR in double-strand break repair. Genetics. 2007;175:1023-33 pubmed..However, this does not account for all of the defects we observed. We propose a novel role for MEI-41 in DSB repair, independent of the Chk1/Chk2-mediated checkpoint response. ..
- Bultman S, Herschkowitz J, Godfrey V, Gebuhr T, Yaniv M, Perou C, et al. Characterization of mammary tumors from Brg1 heterozygous mice. Oncogene. 2008;27:460-8 pubmed..We were also unable to detect a genetic interaction between the Brg1+/- and Rb+/- tumor phenotypes. These latter findings do not support a BRG1-RB interaction in vivo. ..
- Choi J, Lindsey Boltz L, Sancar A. Reconstitution of a human ATR-mediated checkpoint response to damaged DNA. Proc Natl Acad Sci U S A. 2007;104:13301-6 pubmed..This in vitro system provides a useful platform for mechanistic studies of the human DNA damage checkpoint response. ..
- Choi J, Lindsey Boltz L, Sancar A. Cooperative activation of the ATR checkpoint kinase by TopBP1 and damaged DNA. Nucleic Acids Res. 2009;37:1501-9 pubmed publisher..Taken together, these findings suggest a cooperative activation mechanism for the ATR checkpoint kinase by TopBP1 and damaged DNA. ..
- McDonough H, Charles P, Hilliard E, Qian S, Min J, Portbury A, et al. Stress-dependent Daxx-CHIP interaction suppresses the p53 apoptotic program. J Biol Chem. 2009;284:20649-59 pubmed publisher..By abrogating p53-dependent apoptotic pathways and by ubiquitination competitive with Daxx sumoylation, CHIP integrates the proteotoxic stress response of the cell with cell cycle pathways that influence cell survival. ..
- Herold J, Wigle T, Norris J, Lam R, Korboukh V, Gao C, et al. Small-molecule ligands of methyl-lysine binding proteins. J Med Chem. 2011;54:2504-11 pubmed publisher..The first cocrystal structure of a small molecule bound to L3MBTL1 was determined and provides new insights into binding requirements for further ligand design. ..
- Varma D, Chandrasekaran S, Sundin L, Reidy K, Wan X, Chasse D, et al. Recruitment of the human Cdt1 replication licensing protein by the loop domain of Hec1 is required for stable kinetochore-microtubule attachment. Nat Cell Biol. 2012;14:593-603 pubmed publisher..These results support the conclusion that Cdt1 binding to Hec1 is essential for an extended Ndc80 configuration and stable kMT attachment. ..
- Crews S. Control of cell lineage-specific development and transcription by bHLH-PAS proteins. Genes Dev. 1998;12:607-20 pubmed
- Erkmann J, Wagner E, Dong J, Zhang Y, Kutay U, Marzluff W. Nuclear import of the stem-loop binding protein and localization during the cell cycle. Mol Biol Cell. 2005;16:2960-71 pubmed..In contrast, we were unable to identify an in vivo role for Transportin-SR2 in SLBP nuclear localization. Thus, only the Impalpha/Impbeta pathway contributes to SLBP nuclear import in HeLa cells. ..
- Radford S, McMahan S, Blanton H, Sekelsky J. Heteroduplex DNA in meiotic recombination in Drosophila mei-9 mutants. Genetics. 2007;176:63-72 pubmed
- Pearson C, Maddox P, Salmon E, Bloom K. Budding yeast chromosome structure and dynamics during mitosis. J Cell Biol. 2001;152:1255-66 pubmed..These results indicate that the elastic properties of DNA play an as of yet undiscovered role in the poleward movement of chromosome arms. ..
- Reisman D, Strobeck M, Betz B, Sciariotta J, Funkhouser W, Murchardt C, et al. Concomitant down-regulation of BRM and BRG1 in human tumor cell lines: differential effects on RB-mediated growth arrest vs CD44 expression. Oncogene. 2002;21:1196-207 pubmed
- Horikawa T, Yang J, Kondo S, Yoshizaki T, Joab I, Furukawa M, et al. Twist and epithelial-mesenchymal transition are induced by the EBV oncoprotein latent membrane protein 1 and are associated with metastatic nasopharyngeal carcinoma. Cancer Res. 2007;67:1970-8 pubmed..These results suggest that induction of Twist by a human viral oncoprotein LMP1 directly contributes to the metastatic nature of NPC. ..
- Yamane K, Tateishi K, Klose R, Fang J, Fabrizio L, Erdjument Bromage H, et al. PLU-1 is an H3K4 demethylase involved in transcriptional repression and breast cancer cell proliferation. Mol Cell. 2007;25:801-12 pubmed..Furthermore, we reveal that PLU-1-mediated H3K4 demethylase activity plays an important role in the proliferative capacity of breast cancer cells through repression of tumor suppressor genes, including BRCA1. ..
- Lockman K, Taylor J, Mack C. The histone demethylase, Jmjd1a, interacts with the myocardin factors to regulate SMC differentiation marker gene expression. Circ Res. 2007;101:e115-23 pubmed
- Sullivan K, Steiniger M, Marzluff W. A core complex of CPSF73, CPSF100, and Symplekin may form two different cleavage factors for processing of poly(A) and histone mRNAs. Mol Cell. 2009;34:322-32 pubmed publisher..These results suggest that a common core cleavage factor is required for processing of histone and polyadenylated pre-mRNAs. ..
- Anderson M, Haase J, Yeh E, Bloom K. Function and assembly of DNA looping, clustering, and microtubule attachment complexes within a eukaryotic kinetochore. Mol Biol Cell. 2009;20:4131-9 pubmed publisher..The linkage of microtubule-binding to centromere DNA-looping complexes positions the pericentric chromatin loops and stabilizes the dynamic properties of individual kinetochore complexes in mitosis. ..
- Chandler R, Brennan J, Schisler J, Serber D, Patterson C, Magnuson T. ARID1a-DNA interactions are required for promoter occupancy by SWI/SNF. Mol Cell Biol. 2013;33:265-80 pubmed publisher..These findings support the model wherein cooperative interactions among intrinsic subunit-chromatin interaction domains and sequence-specific transcription factors drive SWI/SNF recruitment. ..
- Lee J, Gaddameedhi S, Ozturk N, Ye R, Sancar A. DNA damage-specific control of cell death by cryptochrome in p53-mutant ras-transformed cells. Cancer Res. 2013;73:785-91 pubmed publisher..Our findings establish distinct roles for cryptochromes in intrinsic apoptosis induced by UV mimetic and radiomimetic agents. ..
- Kuwahara Y, Wei D, Durand J, Weissman B. SNF5 reexpression in malignant rhabdoid tumors regulates transcription of target genes by recruitment of SWI/SNF complexes and RNAPII to the transcription start site of their promoters. Mol Cancer Res. 2013;11:251-60 pubmed publisher..Furthermore, because we observe growth inhibition after NOXA expression in MRT cells, the NOXA pathway may provide a novel target with clinical relevancy for treatment of this aggressive disease. ..
- Gullapalli A, Garrett T, Paing M, Griffin C, Yang Y, Trejo J. A role for sorting nexin 2 in epidermal growth factor receptor down-regulation: evidence for distinct functions of sorting nexin 1 and 2 in protein trafficking. Mol Biol Cell. 2004;15:2143-55 pubmed..These studies are the first to demonstrate a function for SNX2 in protein trafficking. ..
- Wennerberg K, Forget M, Ellerbroek S, Arthur W, Burridge K, Settleman J, et al. Rnd proteins function as RhoA antagonists by activating p190 RhoGAP. Curr Biol. 2003;13:1106-15 pubmed..Our results identify p190 RhoGAPs as effectors of Rnd proteins and demonstrate a novel mechanism by which Rnd proteins function as antagonists of RhoA. ..
- Callis T, Cao D, Wang D. Bone morphogenetic protein signaling modulates myocardin transactivation of cardiac genes. Circ Res. 2005;97:992-1000 pubmed..Furthermore, myocardin protein levels were dramatically increased by BMP-2 treatment in cardiomyocytes. These findings suggest myocardin participates in a BMP signaling-dependent cardiac gene transcriptional program. ..
- Johnson M, Villanueva M, Haynes C, Seipel A, Buhler L, Wightman R. Catecholamine exocytosis is diminished in R6/2 Huntington's disease model mice. J Neurochem. 2007;103:2102-10 pubmed..Thus, catecholamine release from tissues isolated from R6/2 mice is diminished because of impaired vesicle loading. ..
- Dronamraju R, Strahl B. A feed forward circuit comprising Spt6, Ctk1 and PAF regulates Pol II CTD phosphorylation and transcription elongation. Nucleic Acids Res. 2014;42:870-81 pubmed publisher..In sum, these results elucidate a new control mechanism for the regulation of RNAPII CTD phosphorylation during transcription elongation that is likely to be highly conserved. ..
- Samson T, Will C, Knoblauch A, Sharek L, von der Mark K, Burridge K, et al. Def-6, a guanine nucleotide exchange factor for Rac1, interacts with the skeletal muscle integrin chain alpha7A and influences myoblast differentiation. J Biol Chem. 2007;282:15730-42 pubmed..Thus, our data suggest a role for Def-6 in the fine regulation of Rac1 during myogenesis with the integrin alpha7A chain guiding this regulation in a spatio-temporal manner. ..
- Yang Y, Gao Y, Mutter Rottmayer L, Zlatanou A, Durando M, Ding W, et al. DNA repair factor RAD18 and DNA polymerase PolÎº confer tolerance of oncogenic DNA replication stress. J Cell Biol. 2017;216:3097-3115 pubmed publisher..Collectively, our results show that the RAD18-PolÎº signaling axis allows tolerance of CDK2-mediated oncogenic stress and may allow neoplastic cells to breach tumorigenic barriers. ..
- Li G, Harton J, Zhu X, Ting J. Downregulation of CIITA function by protein kinase a (PKA)-mediated phosphorylation: mechanism of prostaglandin E, cyclic AMP, and PKA inhibition of class II major histocompatibility complex expression in monocytic lines. Mol Cell Biol. 2001;21:4626-35 pubmed..This is the first demonstration that the posttranslational modification of CIITA mediates inhibition of class II MHC transcription. ..
- Heffernan T, Simpson D, Frank A, Heinloth A, Paules R, Cordeiro Stone M, et al. An ATR- and Chk1-dependent S checkpoint inhibits replicon initiation following UVC-induced DNA damage. Mol Cell Biol. 2002;22:8552-61 pubmed..Taken together, these data suggest that the UVC-induced S checkpoint response of inhibition of replicon initiation is mediated by ATR signaling through Chk-1 and is independent of ATM, Nbs1, and Mre11. ..
- Zika E, Fauquier L, Vandel L, Ting J. Interplay among coactivator-associated arginine methyltransferase 1, CBP, and CIITA in IFN-gamma-inducible MHC-II gene expression. Proc Natl Acad Sci U S A. 2005;102:16321-6 pubmed..These results suggest functional and temporal relationships among CIITA, CARM1, and CBP for IFN-gamma induction of MHC-II. ..
- Shih H, Hales K, Pringle J, Peifer M. Identification of septin-interacting proteins and characterization of the Smt3/SUMO-conjugation system in Drosophila. J Cell Sci. 2002;115:1259-71 pubmed
- Lachiewicz A, Wilkinson T, Groben P, Ollila D, Thomas N. Muir-Torre syndrome. Am J Clin Dermatol. 2007;8:315-9 pubmed
- Yang N, Eckhart A, Xin X, Faber J. CRE-like response element regulates expression of rat alpha 2D-adrenergic receptor gene in vascular smooth muscle. Am J Physiol. 1997;273:H85-95 pubmed..In addition, promoter activity was increased 300% by forskolin or 8-bromoadenosine 3',5'-cyclic monophosphate, indicating that the CRE-like element may regulate alpha 2D-AR expression in vascular tissue...
- Tian Q, Nagpal P, Reed J. Regulation of Arabidopsis SHY2/IAA3 protein turnover. Plant J. 2003;36:643-51 pubmed..The chemical juglone (5-hydroxy-1,4-naphthoquinone) inhibited the interaction, suggesting that peptidyl-prolyl isomerization may mediate auxin-induced SHY2/IAA3 protein turnover. ..
- Erkmann J, Sanchez R, Treichel N, Marzluff W, Kutay U. Nuclear export of metazoan replication-dependent histone mRNAs is dependent on RNA length and is mediated by TAP. RNA. 2005;11:45-58 pubmed..Consistent with this observation, depletion of TAP from Drosophila cells by RNAi resulted in the restriction of mature histone mRNAs to the nucleus. ..
- Civelek M, Wu Y, Pan C, Raulerson C, Ko A, He A, et al. Genetic Regulation of Adipose Gene Expression and Cardio-Metabolic Traits. Am J Hum Genet. 2017;100:428-443 pubmed publisher..These results provide candidate genes for further investigation of their potential roles in adipose biology and in regulating cardio-metabolic traits. ..
- Hinson J, Medlin M, Taylor J, Mack C. Regulation of myocardin factor protein stability by the LIM-only protein FHL2. Am J Physiol Heart Circ Physiol. 2008;295:H1067-H1075 pubmed publisher..Taken together, these data indicate that myocardin factors are regulated by proteasome-mediated degradation and that FHL2 regulates SRF-dependent transcription by multiple mechanisms, including stabilization of myocardin and MRTF-A. ..
- Ting J, Trowsdale J. Genetic control of MHC class II expression. Cell. 2002;109 Suppl:S21-33 pubmed..The most important of these is CIITA, a master controller that orchestrates expression but does not bind directly to the promoter. The transcriptosome complex formed at class II promoters is a model for induction of gene expression. ..
- Bultman S, Gebuhr T, Magnuson T. A Brg1 mutation that uncouples ATPase activity from chromatin remodeling reveals an essential role for SWI/SNF-related complexes in beta-globin expression and erythroid development. Genes Dev. 2005;19:2849-61 pubmed..Not only does this mutation establish a role for Brg1 during organogenesis, it also demonstrates that ATPase activity can be uncoupled from chromatin remodeling. ..
- Haase J, Stephens A, Verdaasdonk J, Yeh E, Bloom K. Bub1 kinase and Sgo1 modulate pericentric chromatin in response to altered microtubule dynamics. Curr Biol. 2012;22:471-81 pubmed publisher..The geometric alteration of all 16 kinetochores and pericentric chromatin reflect global changes in the pericentromeric region and provide mechanisms for mechanically amplifying damage at a single kinetochore microtubule. ..
- Wen K, Dittmer D, Damania B. Disruption of LANA in rhesus rhadinovirus generates a highly lytic recombinant virus. J Virol. 2009;83:9786-802 pubmed publisher..Finally, we also demonstrate that the RRVDeltaLANA/GFP virus fails to establish latency in B cells, as measured by the loss of GFP-positive cells and intracellular viral genomes. ..
- Nguyen A, Taranova O, He J, Zhang Y. DOT1L, the H3K79 methyltransferase, is required for MLL-AF9-mediated leukemogenesis. Blood. 2011;117:6912-22 pubmed publisher..Thus, DOT1L may serve as a potential therapeutic target for the treatment of leukemia caused by MLL translocations. ..
- Whitfield M, Zheng L, Baldwin A, Ohta T, Hurt M, Marzluff W. Stem-loop binding protein, the protein that binds the 3' end of histone mRNA, is cell cycle regulated by both translational and posttranslational mechanisms. Mol Cell Biol. 2000;20:4188-98 pubmed..Regulation of SLBP may account for the posttranscriptional component of the cell cycle regulation of histone mRNA. ..
- Pearson C, Maddox P, Zarzar T, Salmon E, Bloom K. Yeast kinetochores do not stabilize Stu2p-dependent spindle microtubule dynamics. Mol Biol Cell. 2003;14:4181-95 pubmed..These results indicate that Stu2p and Ndc10p differentially function in controlling kinetochore microtubule dynamics necessary for centromere movements. ..
- Myster D, Duronio R. To differentiate or not to differentiate?. Curr Biol. 2000;10:R302-4 pubmed..Equally important is regulated withdrawal from the cell cycle to allow cells to differentiate. Recent evidence supports a direct link between transcriptional regulation of the cell cycle machinery and cell differentiation. ..
- Sullivan E, Santiago C, Parker E, Dominski Z, Yang X, Lanzotti D, et al. Drosophila stem loop binding protein coordinates accumulation of mature histone mRNA with cell cycle progression. Genes Dev. 2001;15:173-87 pubmed..Moreover, dSLBP-dependent processing plays an important role in coupling histone mRNA production with the cell cycle. ..
- Roberts M, Tang S, Stallmann A, Dangl J, Bonardi V. Genetic requirements for signaling from an autoactive plant NB-LRR intracellular innate immune receptor. PLoS Genet. 2013;9:e1003465 pubmed publisher..These data allows us to propose a genetic model that provides insight into an SA-dependent feedback regulation loop, which, surprisingly, includes ADR1-L2. ..
- Richardson R, Alekseev O, Alekseev O, O Rand M. Characterization of the NASP promoter in 3T3 fibroblasts and mouse spermatogenic cells. Gene. 2006;371:52-8 pubmed..Supershift assays further confirmed the presence of Sp1 bound to their respective sites suggesting that Sp1 and Ets are the primary activators of the NASP promoter. ..
- DeLuca J, Gall W, Ciferri C, Cimini D, Musacchio A, Salmon E. Kinetochore microtubule dynamics and attachment stability are regulated by Hec1. Cell. 2006;127:969-82 pubmed..These findings reveal a key role for the Hec1 N terminus in controlling dynamic behavior of kinetochore microtubules. ..
- Subramanian D, Griffith J. Modulation of p53 binding to Holliday junctions and 3-cytosine bulges by phosphorylation events. Biochemistry. 2005;44:2536-44 pubmed..In all cases, phosphorylation of serine 392 occurs; however, reactivation also involves other residues. Together, the results show that p53 DNA binding activity is strongly regulated by the phosphorylation state of the protein. ..
- Zhang H, Wang L, Kao S, Whitehead I, Hart M, Liu B, et al. Functional interaction between the cytoplasmic leucine-zipper domain of HIV-1 gp41 and p115-RhoGEF. Curr Biol. 1999;9:1271-4 pubmed..These findings suggest that an important function of gp41C is to modulate the activity of p115-RhoGEF and they thus reveal a new potential anti-HIV-1 target. ..
- Kizer K, Xiao T, Strahl B. Accelerated nuclei preparation and methods for analysis of histone modifications in yeast. Methods. 2006;40:296-302 pubmed..These new methods are ideal for the analysis of histone modifications and could be applied to the analysis and improved detection of other nuclear proteins. ..
- Laribee R, Krogan N, Xiao T, Shibata Y, Hughes T, Greenblatt J, et al. BUR kinase selectively regulates H3 K4 trimethylation and H2B ubiquitylation through recruitment of the PAF elongation complex. Curr Biol. 2005;15:1487-93 pubmed..Our data reveal a novel function for the BUR kinase in transcriptional regulation through the selective control of histone modifications. ..
- Haque M, Elmore K, Tripathy A, Koc H, Koc E, Spremulli L. Properties of the C-terminal tail of human mitochondrial inner membrane protein Oxa1L and its interactions with mammalian mitochondrial ribosomes. J Biol Chem. 2010;285:28353-62 pubmed publisher..Oxa1L-CTT does not cross-link to proteins decorating the conventional exit tunnel of the bacterial large ribosomal subunit (L22, L23, L24, and L29). ..
- Ozkan Dagliyan I, Chiou Y, Ye R, Hassan B, Ozturk N, Sancar A. Formation of Arabidopsis Cryptochrome 2 photobodies in mammalian nuclei: application as an optogenetic DNA damage checkpoint switch. J Biol Chem. 2013;288:23244-51 pubmed publisher..Finally, we demonstrate that by fusing AtCRY2 to the TopBP1 DNA damage checkpoint protein, light-induced AtCRY2 PBs can be used to activate DNA damage signaling pathway in the absence of DNA damage. ..
- Rosser M, Washburn E, Muchowski P, Patterson C, Cyr D. Chaperone functions of the E3 ubiquitin ligase CHIP. J Biol Chem. 2007;282:22267-77 pubmed..The ability of CHIP to recognize nonnative protein structure may aid in selection of slow folding or misfolded polypeptides for ubiquitination. ..
- Hall J, Lee H, Bunker B, Dorn E, Rogers G, Duronio R, et al. Cdt1 and Cdc6 are destabilized by rereplication-induced DNA damage. J Biol Chem. 2008;283:25356-63 pubmed publisher..We conclude that rereplication-associated DNA damage triggers Cdt1 and Cdc6 ubiquitination and destruction, and propose that this pathway represents an evolutionarily conserved mechanism that minimizes the extent of rereplication. ..
- Yin H, Hu M, Liang X, Ajmo J, Li X, Bataller R, et al. Deletion of SIRT1 from hepatocytes in mice disrupts lipin-1 signaling and aggravates alcoholic fatty liver. Gastroenterology. 2014;146:801-11 pubmed publisher..We compared mice with liver-specific deletion of Sirt1 (Sirt1LKO) mice with their LOX littermates (controls)...
- Blanco I, Kuchenbaecker K, Cuadras D, Wang X, Barrowdale D, de Garibay G, et al. Assessing associations between the AURKA-HMMR-TPX2-TUBG1 functional module and breast cancer risk in BRCA1/2 mutation carriers. PLoS ONE. 2015;10:e0120020 pubmed publisher..Together, the results of this study support the hypothesis of a causative link between altered function of AURKA-HMMR-TPX2-TUBG1 and breast carcinogenesis in BRCA1/2 mutation carriers. ..
- Zhang Y, Xiong Y. Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53. Mol Cell. 1999;3:579-91 pubmed..Our results suggest an ARF-regulated MDM2-dependent p53 stabilization and link the human tumor-associated mutations in ARF with a functional alteration. ..
- Batova I, Richardson R, Widgren E, O Rand M. Analysis of the autoimmune epitopes on human testicular NASP using recombinant and synthetic peptides. Clin Exp Immunol. 2000;121:201-9 pubmed
- Roberts S, Ramsden D. Loading of the nonhomologous end joining factor, Ku, on protein-occluded DNA ends. J Biol Chem. 2007;282:10605-13 pubmed..We suggest a model where Ku utilizes an unusual characteristic of its three-dimensional structure to recognize certain protein-occluded ends without the extensive remodeling of chromatin structure required by other DNA repair pathways. ..
- Rosson G, Bartlett C, Reed W, Weissman B. BRG1 loss in MiaPaCa2 cells induces an altered cellular morphology and disruption in the organization of the actin cytoskeleton. J Cell Physiol. 2005;205:286-94 pubmed..Our results implicate a role for the SWI/SNF complex in the regulation of cellular differentiation. ..
- Carl P, Temple B, Cohen P. Most nuclear systemic autoantigens are extremely disordered proteins: implications for the etiology of systemic autoimmunity. Arthritis Res Ther. 2005;7:R1360-74 pubmed..Finally, the model helps to rationalize the relative rarity of clinically significant autoimmunity despite the prevalence of low specificity/low avidity autoantibodies in normal individuals. ..
- Jacob A, Jordan B, Weinberg R. Organization of amyloid-beta protein precursor intracellular domain-associated protein-1 in the rat brain. J Comp Neurol. 2010;518:3221-36 pubmed publisher..Together with previous work, these data suggest that AIDA-1 serves as a direct signaling link between synapses and the nucleus in adult rat brain. ..
- Kemp M, Lindsey Boltz L, Sancar A. The DNA damage response kinases DNA-dependent protein kinase (DNA-PK) and ataxia telangiectasia mutated (ATM) Are stimulated by bulky adduct-containing DNA. J Biol Chem. 2011;286:19237-46 pubmed publisher..Because the DNA damage response kinase ATM and Rad3-related (ATR) is also stimulated by bulky DNA adducts, we conclude that a common biochemical mechanism exists for activation of DNA-PK, ATM, and ATR by bulky adduct-containing DNA...
- Yamada K, Olsen J, Patel M, Rao K, Walsh C. Functional correction of fanconi anemia group C hematopoietic cells by the use of a novel lentiviral vector. Mol Ther. 2001;3:485-90 pubmed..This is the first description of lentiviral correction of FA cells and suggests that lentiviral vectors may be useful for FA gene transfer. ..
- Unsal Kaçmaz K, Chastain P, Qu P, Minoo P, Cordeiro Stone M, Sancar A, et al. The human Tim/Tipin complex coordinates an Intra-S checkpoint response to UV that slows replication fork displacement. Mol Cell Biol. 2007;27:3131-42 pubmed
- Roman Perez E, Casbas Hernandez P, Pirone J, Rein J, Carey L, Lubet R, et al. Gene expression in extratumoral microenvironment predicts clinical outcome in breast cancer patients. Breast Cancer Res. 2012;14:R51 pubmed..These results document the presence of two distinct subtypes of microenvironment, with Active versus Inactive cancer-adjacent extratumoral microenvironment influencing the aggressiveness and outcome of ER-positive human breast cancers. ..
- Zhu X, Ting J. A 36-amino-acid region of CIITA is an effective inhibitor of CBP: novel mechanism of gamma interferon-mediated suppression of collagen alpha(2)(I) and other promoters. Mol Cell Biol. 2001;21:7078-88 pubmed..Suppression is restored when CIITA is introduced. Other targets of CIITA-mediated promoter suppression include interleukin 4, thymidine kinase, and cyclin D1. ..