Experts and Doctors on hela cells in Chapel Hill, North Carolina, United States


Locale: Chapel Hill, North Carolina, United States
Topic: hela cells

Top Publications

  1. Cao R, Wang L, Wang H, Xia L, Erdjument Bromage H, Tempst P, et al. Role of histone H3 lysine 27 methylation in Polycomb-group silencing. Science. 2002;298:1039-43 pubmed
    ..Methylation on H3-K27 facilitates binding of Polycomb (PC), a component of the PRC1 complex, to histone H3 amino-terminal tail. Thus, these studies establish a link between histone methylation and PcG-mediated gene silencing. ..
  2. Rabinowitz J, Bowles D, Faust S, Ledford J, Cunningham S, Samulski R. Cross-dressing the virion: the transcapsidation of adeno-associated virus serotypes functionally defines subgroups. J Virol. 2004;78:4421-32 pubmed
    ..Exploitation of this approach in generating custom-designed AAV vectors should be of significant value to the field of gene therapy. ..
  3. Kuan Y, Brewer Jensen P, Searles L. Suppressor of sable, a putative RNA-processing protein, functions at the level of transcription. Mol Cell Biol. 2004;24:3734-46 pubmed
    ..Together, these results reveal a link between Su(s), transcription, and pre-mRNA processing. ..
  4. Hall J, Kow E, Nevis K, Lu C, Luce K, Zhong Q, et al. Cdc6 stability is regulated by the Huwe1 ubiquitin ligase after DNA damage. Mol Biol Cell. 2007;18:3340-50 pubmed
    ..Furthermore, yeast cells lacking the Huwe1 ortholog, Tom1, have a similar defect in Cdc6 degradation. Together, these findings demonstrate an important and conserved role for Huwe1 in regulating Cdc6 abundance after DNA damage. ..
  5. Choi J, Sancar A, Lindsey Boltz L. The human ATR-mediated DNA damage checkpoint in a reconstituted system. Methods. 2009;48:3-7 pubmed publisher
    ..This system provides a powerful tool to gain insight into the molecular mechanism of the ATR pathway. Here we describe preparation of the checkpoint components and our specific kinase assay in more detail. ..
  6. Gonzalez C, Wang L, Damania B. Kaposi's sarcoma-associated herpesvirus encodes a viral deubiquitinase. J Virol. 2009;83:10224-33 pubmed publisher
    ..We found that depletion of Orf64 by siRNA resulted in decreased viral lytic transcription and lytic protein expression. These experiments indicate that Orf64 plays a role in KSHV lytic replication. ..
  7. Horowitz E, Finn M, Asokan A. Tyrosine cross-linking reveals interfacial dynamics in adeno-associated viral capsids during infection. ACS Chem Biol. 2012;7:1059-66 pubmed publisher
    ..These adverse effects caused by tyrosine oxidation support the notion that interfacial dynamics at the AAV capsid two-fold symmetry axis play a role in externalization of VP N-termini during infection...
  8. Varma D, Chandrasekaran S, Sundin L, Reidy K, Wan X, Chasse D, et al. Recruitment of the human Cdt1 replication licensing protein by the loop domain of Hec1 is required for stable kinetochore-microtubule attachment. Nat Cell Biol. 2012;14:593-603 pubmed publisher
    ..These results support the conclusion that Cdt1 binding to Hec1 is essential for an extended Ndc80 configuration and stable kMT attachment. ..
  9. Proctor A, Wang Q, Lawrence D, Allbritton N. Development of a peptidase-resistant substrate for single-cell measurement of protein kinase B activation. Anal Chem. 2012;84:7195-202 pubmed publisher
    ..04 ± 0.08 zmol pg(-1) s(-1), respectively, for single LNCaP cells loaded with 4 ± 8 μM of peptide. Peptidase-resistant kinase substrates should find widespread utility in both lysate-based and single-cell assays of kinase activity. ..

More Information

Publications117 found, 100 shown here

  1. Hirsch M, Li C, Bellon I, Yin C, Chavala S, Pryadkina M, et al. Oversized AAV transductifon is mediated via a DNA-PKcs-independent, Rad51C-dependent repair pathway. Mol Ther. 2013;21:2205-16 pubmed publisher
    ..5?kb expression cassette restored dysferlin levels in a dystrophic model. Collectively, oversized AAV genome transduction requires unique DNA repair pathways and offers an alternative, more efficient strategy for large-gene therapy. ..
  2. Choi J, Kim S, Kim S, Kemp M, Sancar A. An Integrated Approach for Analysis of the DNA Damage Response in Mammalian Cells: NUCLEOTIDE EXCISION REPAIR, DNA DAMAGE CHECKPOINT, AND APOPTOSIS. J Biol Chem. 2015;290:28812-21 pubmed publisher
  3. Yang Y, Fan X, Mao M, Song X, Wu P, Zhang Y, et al. Extensive translation of circular RNAs driven by N6-methyladenosine. Cell Res. 2017;27:626-641 pubmed publisher
    ..Our study expands the coding landscape of human transcriptome, and suggests a role of circRNA-derived proteins in cellular responses to environmental stress. ..
  4. Mills C, Suzuki A, Arceci A, Mo J, Duncan A, Salmon E, et al. Nucleolar and spindle-associated protein 1 (NUSAP1) interacts with a SUMO E3 ligase complex during chromosome segregation. J Biol Chem. 2017;292:17178-17189 pubmed publisher
    ..We speculate that NUSAP1 contributes to accurate chromosome segregation by acting as a co-factor for RanBP2-RanGAP1-UBC9 during cell division. ..
  5. Park C, Bessho T, Matsunaga T, Sancar A. Purification and characterization of the XPF-ERCC1 complex of human DNA repair excision nuclease. J Biol Chem. 1995;270:22657-60 pubmed
    ..This complex also nicks supercoiled DNA weakly, and this nicking activity is stimulated by human replication protein A when the DNA contains UV damage. ..
  6. Park C, Sancar A. Formation of a ternary complex by human XPA, ERCC1, and ERCC4(XPF) excision repair proteins. Proc Natl Acad Sci U S A. 1994;91:5017-21 pubmed
    ..We conclude that the XPA damage recognition protein makes a ternary complex with the ERCC1/ERCC4(XPF) heterodimer with a potential nuclease function. ..
  7. Ho K, Marschke K, Tan J, Power S, Wilson E, French F. A complex response element in intron 1 of the androgen-regulated 20-kDa protein gene displays cell type-dependent androgen receptor specificity. J Biol Chem. 1993;268:27226-35 pubmed
    ..Interestingly, a 131-base pair subfragment of In-1c retained ARE specificity in all three cell lines. ..
  8. Wang H, Cao R, Xia L, Erdjument Bromage H, Borchers C, Tempst P, et al. Purification and functional characterization of a histone H3-lysine 4-specific methyltransferase. Mol Cell. 2001;8:1207-17 pubmed
    ..Thus, our study provides a molecular explanation to the differential effects of H3-K4 and H3-K9 methylation on transcription. ..
  9. Berg J, Cheney R. Myosin-X is an unconventional myosin that undergoes intrafilopodial motility. Nat Cell Biol. 2002;4:246-50 pubmed
    ..The localization and movements of M10 strongly suggest that it functions as a motor for intrafilopodial motility. ..
  10. Arthur W, Ellerbroek S, Der C, Burridge K, Wennerberg K. XPLN, a guanine nucleotide exchange factor for RhoA and RhoB, but not RhoC. J Biol Chem. 2002;277:42964-72 pubmed
    ..In conclusion, here we describe a Rho family GEF that can discriminate between the closely related RhoA, RhoB, and RhoC, possibly giving insight to the divergent functions of these three proteins. ..
  11. Shannon K, Canman J, Salmon E. Mad2 and BubR1 function in a single checkpoint pathway that responds to a loss of tension. Mol Biol Cell. 2002;13:3706-19 pubmed
    ..We conclude that reduced tension at kinetochores with a full complement of kinetochore microtubules induces a checkpoint dependent metaphase delay associated with elevated amounts of kinetochore 3F3/2, Bub1, and BubR1 labeling. ..
  12. DeMali K, Barlow C, Burridge K. Recruitment of the Arp2/3 complex to vinculin: coupling membrane protrusion to matrix adhesion. J Cell Biol. 2002;159:881-91 pubmed
    ..The recruitment of the Arp2/3 complex to vinculin may be one mechanism through which actin polymerization and membrane protrusion are coupled to integrin-mediated adhesion. ..
  13. DeLuca J, Howell B, Canman J, Hickey J, Fang G, Salmon E. Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores. Curr Biol. 2003;13:2103-9 pubmed
    ..Our observations support a model in which Nuf2 and Hec1 function to prevent microtubule-dependent stripping of Mad1 and Mad2 from kinetochores that have not yet formed stable kinetochore-microtubule attachments. ..
  14. O Connor W, Harton J, Zhu X, Linhoff M, Ting J. Cutting edge: CIAS1/cryopyrin/PYPAF1/NALP3/CATERPILLER 1.1 is an inducible inflammatory mediator with NF-kappa B suppressive properties. J Immunol. 2003;171:6329-33 pubmed
    ..These data suggest CIAS1/cryopyrin may act as a key regulator of inflammation, induced to dampen NF-kappaB-dependent proinflammatory signals. ..
  15. Paing M, Temple B, Trejo J. A tyrosine-based sorting signal regulates intracellular trafficking of protease-activated receptor-1: multiple regulatory mechanisms for agonist-induced G protein-coupled receptor internalization. J Biol Chem. 2004;279:21938-47 pubmed
    ..This study is the first to describe a function for a tyrosine-based motif, YXX, in GPCR internalization and reveal novel complexities in the regulation of GPCR trafficking. ..
  16. Alekseev O, Widgren E, Richardson R, O Rand M. Association of NASP with HSP90 in mouse spermatogenic cells: stimulation of ATPase activity and transport of linker histones into nuclei. J Biol Chem. 2005;280:2904-11 pubmed
    ..NASP-H1 is subsequently released from the complex and translocates to the nucleus where the H1 is released for binding to the DNA. ..
  17. DeLuca J, Dong Y, Hergert P, Strauss J, Hickey J, Salmon E, et al. Hec1 and nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites. Mol Biol Cell. 2005;16:519-31 pubmed
    ..Our results indicate that Nuf2 and Hec1 are required for organization of stable microtubule plus-end binding sites in the outer plate that are needed for the sustained poleward forces required for biorientation at kinetochores. ..
  18. Askew E, Gampe R, Stanley T, Faggart J, Wilson E. Modulation of androgen receptor activation function 2 by testosterone and dihydrotestosterone. J Biol Chem. 2007;282:25801-16 pubmed
    ..We conclude that T is a weaker androgen than DHT because of less favorable T-dependent AR FXXLF and coactivator LXXLL motif interactions at AF2. ..
  19. Dan H, Cooper M, Cogswell P, Duncan J, Ting J, Baldwin A. Akt-dependent regulation of NF-{kappa}B is controlled by mTOR and Raptor in association with IKK. Genes Dev. 2008;22:1490-500 pubmed publisher
    ..The results provide insight into the effects of Akt/mTOR-dependent signaling on gene expression and into the therapeutic action of rapamycin. ..
  20. Lu K, Craft S, Nakamura J, Moeller B, Swenberg J. Use of LC-MS/MS and stable isotopes to differentiate hydroxymethyl and methyl DNA adducts from formaldehyde and nitrosodimethylamine. Chem Res Toxicol. 2012;25:664-75 pubmed publisher
    ..The use of highly sensitive LC-MS/MS and isotope labeled compounds for exposure solves these challenges and provides mechanistic insights on the formation and role of these DNA adducts...
  21. Melvin A, Dumberger L, Woss G, Waters M, Allbritton N. Identification of a p53-based portable degron based on the MDM2-p53 binding region. Analyst. 2016;141:570-8 pubmed publisher
    ..This analysis identified the five amino acid sequence, KGSYG, corresponding to residues 105-108 with an added N-terminal lysine, as a portable degron since this sequence demonstrated the most rapid ubiquitination kinetics. ..
  22. Wang H, Wang L, Erdjument Bromage H, Vidal M, Tempst P, Jones R, et al. Role of histone H2A ubiquitination in Polycomb silencing. Nature. 2004;431:873-8 pubmed
    ..Thus, our studies identify the H2A ubiquitin ligase, and link H2A ubiquitination to Polycomb silencing. ..
  23. Bohil A, Robertson B, Cheney R. Myosin-X is a molecular motor that functions in filopodia formation. Proc Natl Acad Sci U S A. 2006;103:12411-6 pubmed
    ..Together, these data demonstrate that Myo10 is a molecular motor that functions in filopodia formation. ..
  24. Kondo S, Seo S, Yoshizaki T, Wakisaka N, Furukawa M, Joab I, et al. EBV latent membrane protein 1 up-regulates hypoxia-inducible factor 1alpha through Siah1-mediated down-regulation of prolyl hydroxylases 1 and 3 in nasopharyngeal epithelial cells. Cancer Res. 2006;66:9870-7 pubmed
    ..Thus, Siah1 is implicated in the regulation of HIF1alpha and is involved in a recently appreciated aspect of EBV-mediated tumorigenesis, namely, the angiogenesis process triggered by LMP1. ..
  25. Wu S, Zhang Y. Cyclin-dependent kinase 1 (CDK1)-mediated phosphorylation of enhancer of zeste 2 (Ezh2) regulates its stability. J Biol Chem. 2011;286:28511-9 pubmed publisher
    ..Collectively, these results establish a novel function for CDK1-mediated Ezh2 phosphorylation and provide a mechanism by which Ezh2 protein levels can be regulated in cells. ..
  26. Tuupanen S, Yan J, Turunen M, Gylfe A, Kaasinen E, Li L, et al. Characterization of the colorectal cancer-associated enhancer MYC-335 at 8q24: the role of rs67491583. Cancer Genet. 2012;205:25-33 pubmed publisher
  27. Yang X, Sabath I, Debski J, Kaus Drobek M, Dadlez M, Marzluff W, et al. A complex containing the CPSF73 endonuclease and other polyadenylation factors associates with U7 snRNP and is recruited to histone pre-mRNA for 3'-end processing. Mol Cell Biol. 2013;33:28-37 pubmed publisher
  28. Nick McElhinny S, Snowden C, McCarville J, Ramsden D. Ku recruits the XRCC4-ligase IV complex to DNA ends. Mol Cell Biol. 2000;20:2996-3003 pubmed
    ..We have therefore defined cell-free conditions that reflect the genetic requirement for ligase IV in cellular end joining and consequently can explain in molecular terms why this factor is required. ..
  29. Wang D, Westerheide S, Hanson J, Baldwin A. Tumor necrosis factor alpha-induced phosphorylation of RelA/p65 on Ser529 is controlled by casein kinase II. J Biol Chem. 2000;275:32592-7 pubmed
    ..These data may explain the ability of CKII to modulate cell growth and demonstrate a mechanism whereby CKII can function in an inducible manner. ..
  30. He B, Gampe R, Hnat A, Faggart J, Minges J, French F, et al. Probing the functional link between androgen receptor coactivator and ligand-binding sites in prostate cancer and androgen insensitivity. J Biol Chem. 2006;281:6648-63 pubmed
    ..The results suggest a bidirectional structural relay between bound ligand and coactivator that establishes AR functional potency in vivo...
  31. Adli M, Baldwin A. IKK-i/IKKepsilon controls constitutive, cancer cell-associated NF-kappaB activity via regulation of Ser-536 p65/RelA phosphorylation. J Biol Chem. 2006;281:26976-84 pubmed
    ..The data indicate a role for IKK-i/IKKepsilon in controlling proliferation of certain cancer cells through regulation of constitutive NF-kappaB activity. ..
  32. Leonard S, Ferry Leeper P, Mailman R. Low affinity binding of the classical D1 antagonist SCH23390 in rodent brain: potential interaction with A2A and D2-like receptors. Brain Res. 2006;1117:25-37 pubmed
    ..Therefore, we suggest that the heteromerization of D(2) and A(2A) receptors reported previously in vitro also may occur in the brain of both rats and mice. ..
  33. DeLuca J, Gall W, Ciferri C, Cimini D, Musacchio A, Salmon E. Kinetochore microtubule dynamics and attachment stability are regulated by Hec1. Cell. 2006;127:969-82 pubmed
    ..These findings reveal a key role for the Hec1 N terminus in controlling dynamic behavior of kinetochore microtubules. ..
  34. Wang Y, Cheong C, Hall T, Wang Z. Engineering splicing factors with designed specificities. Nat Methods. 2009;6:825-30 pubmed publisher
    ..Our approach permitted the creation of artificial factors to target virtually any pre-mRNA, providing a strategy to study splicing regulation and to manipulate disease-associated splicing events. ..
  35. Aghajanian A, Wittchen E, Campbell S, Burridge K. Direct activation of RhoA by reactive oxygen species requires a redox-sensitive motif. PLoS ONE. 2009;4:e8045 pubmed publisher
    ..This mechanism of regulation may be particularly relevant in pathological conditions where ROS are generated and the cellular redox-balance altered, such as in asthma and ischemia-reperfusion injury. ..
  36. Kim K, Lee B, Kim J, Choi J, Kim J, Xiong Y, et al. Linker Histone H1.2 cooperates with Cul4A and PAF1 to drive H4K31 ubiquitylation-mediated transactivation. Cell Rep. 2013;5:1690-703 pubmed publisher
    ..These data define an expanded role for H1 in regulating gene transcription and illustrate its dependence on the elongation competence of RNAPII. ..
  37. Slevin M, Meaux S, Welch J, Bigler R, Miliani de Marval P, Su W, et al. Deep sequencing shows multiple oligouridylations are required for 3' to 5' degradation of histone mRNAs on polyribosomes. Mol Cell. 2014;53:1020-30 pubmed publisher
    ..Knockdown of No-go decay factors also slowed histone mRNA degradation, suggesting a role in removing ribosomes from partially degraded mRNAs. ..
  38. Lindsey Boltz L, Bermudez V, Hurwitz J, Sancar A. Purification and characterization of human DNA damage checkpoint Rad complexes. Proc Natl Acad Sci U S A. 2001;98:11236-41 pubmed
    ..These findings constitute biochemical support for models regarding the roles of checkpoint Rads as damage sensors in the DNA damage checkpoint response of human cells. ..
  39. Wagner E, Marzluff W. ZFP100, a component of the active U7 snRNP limiting for histone pre-mRNA processing, is required for entry into S phase. Mol Cell Biol. 2006;26:6702-12 pubmed
  40. Keedy K, Archin N, Gates A, Espeseth A, Hazuda D, Margolis D. A limited group of class I histone deacetylases acts to repress human immunodeficiency virus type 1 expression. J Virol. 2009;83:4749-56 pubmed publisher
    ..Together, these results suggest that HDAC inhibitors specific for a limited number of class I HDACs may offer a targeted approach to the disruption of persistent HIV-1 infection. ..
  41. Xiao P, Samulski R. Cytoplasmic trafficking, endosomal escape, and perinuclear accumulation of adeno-associated virus type 2 particles are facilitated by microtubule network. J Virol. 2012;86:10462-73 pubmed publisher
  42. Lane K, Yu Y, Lackey P, Chen X, Marzluff W, Cook J. Cell cycle-regulated protein abundance changes in synchronously proliferating HeLa cells include regulation of pre-mRNA splicing proteins. PLoS ONE. 2013;8:e58456 pubmed publisher
    ..Comparison of this dataset to several other proteomic datasets sheds light on global mechanisms of cell cycle phase transitions and underscores the importance of both phosphorylation and ubiquitination in cell cycle changes. ..
  43. Dagliyan O, Shirvanyants D, Karginov A, Ding F, Fee L, Chandrasekaran S, et al. Rational design of a ligand-controlled protein conformational switch. Proc Natl Acad Sci U S A. 2013;110:6800-4 pubmed publisher
    ..The rational creation of uniRapR exemplifies the strength of computational protein design, and offers a powerful means for targeted activation of many pathways to study signaling in living organisms. ..
  44. Su S, Minges J, Grossman G, Blackwelder A, Mohler J, Wilson E. Proto-oncogene activity of melanoma antigen-A11 (MAGE-A11) regulates retinoblastoma-related p107 and E2F1 proteins. J Biol Chem. 2013;288:24809-24 pubmed publisher
  45. Lu Y, Mauger D, Goldstein D, Urban T, Weeks K, Bradrick S. IFNL3 mRNA structure is remodeled by a functional non-coding polymorphism associated with hepatitis C virus clearance. Sci Rep. 2015;5:16037 pubmed publisher
    ..These data illustrate that non-coding genetic variants can have significant functional effects by impacting RNA structure. ..
  46. Hall J, Lee H, Bunker B, Dorn E, Rogers G, Duronio R, et al. Cdt1 and Cdc6 are destabilized by rereplication-induced DNA damage. J Biol Chem. 2008;283:25356-63 pubmed publisher
    ..We conclude that rereplication-associated DNA damage triggers Cdt1 and Cdc6 ubiquitination and destruction, and propose that this pathway represents an evolutionarily conserved mechanism that minimizes the extent of rereplication. ..
  47. Conti B, Davis B, Zhang J, O Connor W, Williams K, Ting J. CATERPILLER 16.2 (CLR16.2), a novel NBD/LRR family member that negatively regulates T cell function. J Biol Chem. 2005;280:18375-85 pubmed
    ..We propose that CLR16.2 serves to attenuate T cell activation via TCR and co-stimulatory molecules, and its reduction during T cell stimulation allows the ensuing cellular activation. ..
  48. Adli M, Merkhofer E, Cogswell P, Baldwin A. IKKalpha and IKKbeta each function to regulate NF-kappaB activation in the TNF-induced/canonical pathway. PLoS ONE. 2010;5:e9428 pubmed publisher
    ..These results demonstrate the importance of IKKalpha in canonical NF-kappaB activation, downstream of cytokine treatment of cells. The experiments suggest that IKKalpha will be a therapeutic target in inflammatory disorders. ..
  49. Shimakami T, Yamane D, Jangra R, Kempf B, Spaniel C, Barton D, et al. Stabilization of hepatitis C virus RNA by an Ago2-miR-122 complex. Proc Natl Acad Sci U S A. 2012;109:941-6 pubmed publisher
    ..miR-122 thus acts in an unconventional fashion to stabilize HCV RNA and slow its decay, expanding the repertoire of mechanisms by which miRNAs modulate gene expression. ..
  50. Antoniak S, Owens A, Baunacke M, Williams J, Lee R, Weithäuser A, et al. PAR-1 contributes to the innate immune response during viral infection. J Clin Invest. 2013;123:1310-22 pubmed publisher
    ..Our results indicate that the tissue factor/thrombin/PAR-1 pathway enhances IFN-? expression and contributes to the innate immune response during single-stranded RNA viral infection. ..
  51. Park S, Ahmad F, Um J, Brown A, Xu X, Kang H, et al. Specific Sirt1 Activator-mediated Improvement in Glucose Homeostasis Requires Sirt1-Independent Activation of AMPK. EBioMedicine. 2017;18:128-138 pubmed publisher
    ..Interestingly, weight loss induced by SRT1720 is not sufficient to improve glucose tolerance. Therefore, contrary to current belief, the metabolic effects produced by SRT1720 require AMPK, which can be activated independently of Sirt1. ..
  52. Chin L, Raynor M, Wei X, Chen H, Li L. Hrs interacts with sorting nexin 1 and regulates degradation of epidermal growth factor receptor. J Biol Chem. 2001;276:7069-78 pubmed
    ..These results suggest that Hrs may regulate lysosomal trafficking through its interaction with SNX1. ..
  53. Roberts S, Ramsden D. Loading of the nonhomologous end joining factor, Ku, on protein-occluded DNA ends. J Biol Chem. 2007;282:10605-13 pubmed
    ..We suggest a model where Ku utilizes an unusual characteristic of its three-dimensional structure to recognize certain protein-occluded ends without the extensive remodeling of chromatin structure required by other DNA repair pathways. ..
  54. Dong B, Valencia C, Liu R. Ca(2+)/calmodulin directly interacts with the pleckstrin homology domain of AKT1. J Biol Chem. 2007;282:25131-40 pubmed
    ..CaM is directly involved in regulating the functions of AKT1, presumably by releasing the activated AKT1 from the plasma membrane and/or prohibiting it from re-association with phosphoinositides on plasma membrane. ..
  55. Varma D, Wan X, Cheerambathur D, Gassmann R, Suzuki A, Lawrimore J, et al. Spindle assembly checkpoint proteins are positioned close to core microtubule attachment sites at kinetochores. J Cell Biol. 2013;202:735-46 pubmed publisher
    ..These results reveal how checkpoint proteins are integrated within the substructure of the kinetochore and will aid in understanding the coordination of microtubule attachment and checkpoint signaling during chromosome segregation. ..
  56. Ning S, Huye L, Pagano J. Regulation of the transcriptional activity of the IRF7 promoter by a pathway independent of interferon signaling. J Biol Chem. 2005;280:12262-70 pubmed
    ..A regulatory network between type I IFNs and IRF7 is proposed. The distinct pathways may reflect special roles for an efficient antiviral response at different stages of virus infection. ..
  57. Thelin W, Chen Y, Gentzsch M, Kreda S, Sallee J, Scarlett C, et al. Direct interaction with filamins modulates the stability and plasma membrane expression of CFTR. J Clin Invest. 2007;117:364-74 pubmed
    ..In addition, we elucidate the molecular defect associated with the S13F mutation. ..
  58. Cakmakci N, Lerner R, Wagner E, Zheng L, Marzluff W. SLIP1, a factor required for activation of histone mRNA translation by the stem-loop binding protein. Mol Cell Biol. 2008;28:1182-94 pubmed
    ..SLIP1 may function by bridging the 3' end of the histone mRNA with the 5' end of the mRNA, similar to the mechanism of translation of polyadenylated mRNAs. ..
  59. Cao R, Wang H, He J, Erdjument Bromage H, Tempst P, Zhang Y. Role of hPHF1 in H3K27 methylation and Hox gene silencing. Mol Cell Biol. 2008;28:1862-72 pubmed
    ..Therefore, our results reveal hPHF1 as a component of a novel EED-EZH2 complex and demonstrate its important role in H3K27 methylation and Hox gene silencing. ..
  60. Koseoglu M, Graves L, Marzluff W. Phosphorylation of threonine 61 by cyclin a/Cdk1 triggers degradation of stem-loop binding protein at the end of S phase. Mol Cell Biol. 2008;28:4469-79 pubmed publisher
    ..We conclude that the increase in cyclin A/Cdk1 activity at the end of S phase triggers degradation of SLBP at S/G(2). ..
  61. Gaulton K, Nammo T, Pasquali L, Simon J, Giresi P, Fogarty M, et al. A map of open chromatin in human pancreatic islets. Nat Genet. 2010;42:255-9 pubmed publisher
    ..These findings illuminate the tissue-specific organization of cis-regulatory elements and show that FAIRE-seq can guide the identification of regulatory variants underlying disease susceptibility. ..
  62. Wheeler T, Chin L, Li Y, Roudabush F, Li L. Regulation of synaptophysin degradation by mammalian homologues of seven in absentia. J Biol Chem. 2002;277:10273-82 pubmed
    ..Our findings indicate that Siah proteins function as E3 ubiquitin-protein ligases to regulate the ubiquitination and degradation of synaptophysin. ..
  63. Yang X, Purdy M, Marzluff W, Dominski Z. Characterization of 3'hExo, a 3' exonuclease specifically interacting with the 3' end of histone mRNA. J Biol Chem. 2006;281:30447-54 pubmed
    ..3'hExo removes 3' overhangs of small interfering RNAs, whereas the double-stranded region is resistant to the enzymatic activity. ..
  64. Nikolaishvili Feinberg N, Jenkins G, Nevis K, Staus D, Scarlett C, Unsal Kaçmaz K, et al. Ubiquitylation of proliferating cell nuclear antigen and recruitment of human DNA polymerase eta. Biochemistry. 2008;47:4141-50 pubmed publisher
    ..These results demonstrate that PCNA ubiquitylation at K164 of PCNA is not required in vitro for pol eta to gain access to replication complexes at forks stalled by T (wedge)T and to catalyze TLS across this dimer. ..
  65. Furukawa M, Zhang Y, McCarville J, Ohta T, Xiong Y. The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1. Mol Cell Biol. 2000;20:8185-97 pubmed
    ..This pathway may be commonly utilized for the assembly of other cullin ligases. ..
  66. Yue W, Shackelford J, Pagano J. cdc2/cyclin B1-dependent phosphorylation of EBNA2 at Ser243 regulates its function in mitosis. J Virol. 2006;80:2045-50 pubmed publisher
    ..Our data implicate EBNA2 Ser243 as a cdc2/cyclin B1 site of phosphorylation important for EBNA2's cotranscriptional function in mitosis...
  67. Choi J, Lindsey Boltz L, Sancar A. Reconstitution of a human ATR-mediated checkpoint response to damaged DNA. Proc Natl Acad Sci U S A. 2007;104:13301-6 pubmed
    ..This in vitro system provides a useful platform for mechanistic studies of the human DNA damage checkpoint response. ..
  68. Valencia C, Cotten S, Duan J, Liu R. Modulation of nucleobindin-1 and nucleobindin-2 by caspases. FEBS Lett. 2008;582:286-90 pubmed
    ..Significantly, the caspase cleavage sites are located exactly at one of the Ca(2+)-binding EF-hand motifs. Our results suggest that the functions of nucleobindins could be modulated by caspase-mediated cleavage in apoptosis. ..
  69. Choi J, Lindsey Boltz L, Kemp M, Mason A, Wold M, Sancar A. Reconstitution of RPA-covered single-stranded DNA-activated ATR-Chk1 signaling. Proc Natl Acad Sci U S A. 2010;107:13660-5 pubmed publisher
    ..We also find that TopBP1 is recruited to RPA-ssDNA in a manner dependent on ATRIP and that the N terminus of TopBP1 is required for efficient recruitment and activation of ATR kinase. ..
  70. Dubash A, Guilluy C, Srougi M, Boulter E, Burridge K, Garcia Mata R. The small GTPase RhoA localizes to the nucleus and is activated by Net1 and DNA damage signals. PLoS ONE. 2011;6:e17380 pubmed publisher
    ..These results demonstrate the existence of a functional Net1/RhoA signaling pathway within the nucleus of the cell and implicate them in the DNA damage response. ..
  71. Rothbart S, Krajewski K, Nady N, Tempel W, Xue S, Badeaux A, et al. Association of UHRF1 with methylated H3K9 directs the maintenance of DNA methylation. Nat Struct Mol Biol. 2012;19:1155-60 pubmed publisher
    ..Collectively, our results define a previously unknown link between H3K9 methylation and the faithful epigenetic inheritance of DNA methylation, establishing a notable mitotic role for UHRF1 in this process. ..
  72. Dobes N, Dhopeshwarkar R, Henley W, Ramsey J, Sims C, Allbritton N. Laser-based directed release of array elements for efficient collection into targeted microwells. Analyst. 2013;138:831-8 pubmed publisher
    ..Cell viability based on single-cell cloning efficiency at 72 h post collection was 77%. ..
  73. Park C, Mu D, Reardon J, Sancar A. The general transcription-repair factor TFIIH is recruited to the excision repair complex by the XPA protein independent of the TFIIE transcription factor. J Biol Chem. 1995;270:4896-902 pubmed
    ..This latter interaction has no apparent role in general excision repair but may be relevant in the transcription-coupled repair reaction. ..
  74. He B, Bai S, Hnat A, Kalman R, Minges J, Patterson C, et al. An androgen receptor NH2-terminal conserved motif interacts with the COOH terminus of the Hsp70-interacting protein (CHIP). J Biol Chem. 2004;279:30643-53 pubmed
  75. Furukawa M, Xiong Y. BTB protein Keap1 targets antioxidant transcription factor Nrf2 for ubiquitination by the Cullin 3-Roc1 ligase. Mol Cell Biol. 2005;25:162-71 pubmed
    ..These results may reconcile previously observed cytoplasmic sequestration of NRF2 by KEAP1 and suggest a possible regulatory step between KEAP1-NRF2 binding and NRF2 degradation. ..
  76. Tsukada Y, Fang J, Erdjument Bromage H, Warren M, Borchers C, Tempst P, et al. Histone demethylation by a family of JmjC domain-containing proteins. Nature. 2006;439:811-6 pubmed
    ..Thus, we identify the JmjC domain as a novel demethylase signature motif and uncover a protein demethylation mechanism that is conserved from yeast to human. ..
  77. Horikawa T, Yang J, Kondo S, Yoshizaki T, Joab I, Furukawa M, et al. Twist and epithelial-mesenchymal transition are induced by the EBV oncoprotein latent membrane protein 1 and are associated with metastatic nasopharyngeal carcinoma. Cancer Res. 2007;67:1970-8 pubmed
    ..These results suggest that induction of Twist by a human viral oncoprotein LMP1 directly contributes to the metastatic nature of NPC. ..
  78. Bishai E, Sidhu G, Li W, Dhillon J, Bohil A, Cheney R, et al. Myosin-X facilitates Shigella-induced membrane protrusions and cell-to-cell spread. Cell Microbiol. 2013;15:353-367 pubmed publisher
    ..We conclude thatMyo10 generates the force to enhance bacterial-induced protrusions by binding its head region to actin filaments and its PH tail domain to the peripheral membrane...
  79. Ashburner B, Westerheide S, Baldwin A. The p65 (RelA) subunit of NF-kappaB interacts with the histone deacetylase (HDAC) corepressors HDAC1 and HDAC2 to negatively regulate gene expression. Mol Cell Biol. 2001;21:7065-77 pubmed
    ..Moreover, it suggests that the association of NF-kappaB with the HDAC1 and HDAC2 corepressor proteins functions to repress expression of NF-kappaB-regulated genes as well as to control the induced level of expression of these genes. ..
  80. Rodriguez O, Cheney R. Human myosin-Vc is a novel class V myosin expressed in epithelial cells. J Cell Sci. 2002;115:991-1004 pubmed
    ..As a class V myosin of epithelial cells, Myo5c is likely to power actin-based membrane trafficking in many physiologically crucial tissues of the human body. ..
  81. Deming P, Flores K, Downes C, Paules R, Kaufmann W. ATR enforces the topoisomerase II-dependent G2 checkpoint through inhibition of Plk1 kinase. J Biol Chem. 2002;277:36832-8 pubmed
    ..Moreover, the results demonstrate an important role for the topoisomerase II-dependent G(2) checkpoint in the preservation of human genomic stability. ..
  82. Dominski Z, Yang X, Kaygun H, Dadlez M, Marzluff W. A 3' exonuclease that specifically interacts with the 3' end of histone mRNA. Mol Cell. 2003;12:295-305 pubmed
    ..These features make 3'hExo a primary candidate for the exonuclease that initiates rapid decay of histone mRNA upon completion and/or inhibition of DNA replication. ..
  83. Solomon I, Hager J, Safi R, McDonnell D, Redinbo M, Ortlund E. Crystal structure of the human LRH-1 DBD-DNA complex reveals Ftz-F1 domain positioning is required for receptor activity. J Mol Biol. 2005;354:1091-102 pubmed
    ..Taken together, these results indicate that subtle structural changes in a nuclear receptor DBD can exert long-range functional effects on the LBD of a receptor, and significantly impact transcriptional regulation. ..
  84. Newman M, Thomson J, Hammond S. Lin-28 interaction with the Let-7 precursor loop mediates regulated microRNA processing. RNA. 2008;14:1539-49 pubmed publisher
    ..Our findings outline a microRNA post-transcriptional regulatory network and establish a novel role for the miRNA precursor loop in the regulated production of mature Let-7. ..
  85. Reardon J, Bessho T, Kung H, Bolton P, Sancar A. In vitro repair of oxidative DNA damage by human nucleotide excision repair system: possible explanation for neurodegeneration in xeroderma pigmentosum patients. Proc Natl Acad Sci U S A. 1997;94:9463-8 pubmed
    ..Our results suggest that XP neurological disease may be caused by defective repair of lesions that are produced in nerve cells by reactive oxygen species generated as by-products of an active oxidative metabolism. ..
  86. Ozgur S, Sancar A. Purification and properties of human blue-light photoreceptor cryptochrome 2. Biochemistry. 2003;42:2926-32 pubmed
    ..These findings reveal new properties of this protein already known to function as a circadian photoreceptor and a light-independent negative transcriptional regulator of the clock genes. ..
  87. Garcia Mata R, Dubash A, Sharek L, Carr H, Frost J, Burridge K. The nuclear RhoA exchange factor Net1 interacts with proteins of the Dlg family, affects their localization, and influences their tumor suppressor activity. Mol Cell Biol. 2007;27:8683-97 pubmed
    ..Together, our results suggest that the interaction between Net1 and Dlg1 may contribute to the mechanism of Net1-mediated transformation. ..
  88. Nevis K, Cordeiro Stone M, Cook J. Origin licensing and p53 status regulate Cdk2 activity during G(1). Cell Cycle. 2009;8:1952-63 pubmed
    ..These results demonstrate dependence on origin licensing for multiple events required for G(1) progression, and suggest a mechanism to prevent premature S phase entry that functions in normal cells but not in p53-deficient cells. ..
  89. Chandrasekaran S, Tan T, Hall J, Cook J. Stress-stimulated mitogen-activated protein kinases control the stability and activity of the Cdt1 DNA replication licensing factor. Mol Cell Biol. 2011;31:4405-16 pubmed publisher
    ..These findings support a model in which MAP kinase activity in G(2) promotes reaccumulation of a low-activity Cdt1 isoform after replication is complete. ..
  90. Tan J, Hall S, Hamil K, Grossman G, Petrusz P, Liao J, et al. Protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1) is a nuclear receptor coregulator expressed in human testis. Mol Endocrinol. 2000;14:14-26 pubmed
    ..In addition, PIAS1 was expressed in spermatogenic cells. The results suggest that PIAS1 functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. ..
  91. Erkmann J, Wagner E, Dong J, Zhang Y, Kutay U, Marzluff W. Nuclear import of the stem-loop binding protein and localization during the cell cycle. Mol Biol Cell. 2005;16:2960-71 pubmed
    ..In contrast, we were unable to identify an in vivo role for Transportin-SR2 in SLBP nuclear localization. Thus, only the Impalpha/Impbeta pathway contributes to SLBP nuclear import in HeLa cells. ..
  92. Kaygun H, Marzluff W. Regulated degradation of replication-dependent histone mRNAs requires both ATR and Upf1. Nat Struct Mol Biol. 2005;12:794-800 pubmed
    ..Here we show that regulated degradation of histone mRNAs requires Upf1, a key regulator of the nonsense-mediated decay pathway, and ATR, a key regulator of the DNA damage checkpoint pathway activated during replication stress...