Experts and Doctors on flavoproteins in Chapel Hill, North Carolina, United States

Summary

Locale: Chapel Hill, North Carolina, United States
Topic: flavoproteins

Top Publications

  1. Ozturk N, Lee J, Gaddameedhi S, Sancar A. Loss of cryptochrome reduces cancer risk in p53 mutant mice. Proc Natl Acad Sci U S A. 2009;106:2841-6 pubmed publisher
    ..These results suggest alternative therapeutic approaches in management of cancers associated with a p53 mutation. ..
  2. Worthington E, Kavakli I, Berrocal Tito G, Bondo B, Sancar A. Purification and characterization of three members of the photolyase/cryptochrome family blue-light photoreceptors from Vibrio cholerae. J Biol Chem. 2003;278:39143-54 pubmed publisher
    ..In addition, VcCry1 exhibits RNA binding activity and co-purifies with an RNA of 60-70 nucleotides in length...
  3. Zhao S, Sancar A. Human blue-light photoreceptor hCRY2 specifically interacts with protein serine/threonine phosphatase 5 and modulates its activity. Photochem Photobiol. 1997;66:727-31 pubmed
    ..We found that hCRY2, but not the highly homologous (6-4) photolyase, inhibits the phosphatase activity of PP5. This inhibition may be on the pathway of blue-light signal transduction reaction in humans. ..
  4. Ozturk N, Selby C, Song S, Ye R, Tan C, Kao Y, et al. Comparative photochemistry of animal type 1 and type 4 cryptochromes. Biochemistry. 2009;48:8585-93 pubmed publisher
    ..Finally, we demonstrate that in contrast to animal type 2 CRYs and Arabidopsis CRY1 neither insect type 1 nor type 4 CRYs have autokinase activities. ..
  5. Ozturk N, Song S, Selby C, Sancar A. Animal type 1 cryptochromes. Analysis of the redox state of the flavin cofactor by site-directed mutagenesis. J Biol Chem. 2008;283:3256-63 pubmed
  6. Ozturk N, Selby C, Annayev Y, Zhong D, Sancar A. Reaction mechanism of Drosophila cryptochrome. Proc Natl Acad Sci U S A. 2011;108:516-21 pubmed publisher
    ..These findings lead to a plausible model for circadian photoreception/phototransduction in Drosophila. ..
  7. Wu Y, Frey D, Lungu O, Jaehrig A, Schlichting I, Kuhlman B, et al. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009;461:104-8 pubmed publisher
    ..A PA-Rac crystal structure and modelling revealed LOV-Rac interactions that will facilitate extension of this photoactivation approach to other proteins...
  8. Partch C, Sancar A. Photochemistry and photobiology of cryptochrome blue-light photopigments: the search for a photocycle. Photochem Photobiol. 2005;81:1291-304 pubmed
    ..In particular, the role of the unique C-terminal domain in cryptochrome phototransduction is discussed. ..
  9. Sancar A. Regulation of the mammalian circadian clock by cryptochrome. J Biol Chem. 2004;279:34079-82 pubmed

More Information

Publications25

  1. Ozgur S, Sancar A. Purification and properties of human blue-light photoreceptor cryptochrome 2. Biochemistry. 2003;42:2926-32 pubmed
    ..These findings reveal new properties of this protein already known to function as a circadian photoreceptor and a light-independent negative transcriptional regulator of the clock genes. ..
  2. Selby C, Thompson C, Schmitz T, Van Gelder R, Sancar A. Functional redundancy of cryptochromes and classical photoreceptors for nonvisual ocular photoreception in mice. Proc Natl Acad Sci U S A. 2000;97:14697-702 pubmed
  3. Thresher R, Vitaterna M, Miyamoto Y, Kazantsev A, Hsu D, Petit C, et al. Role of mouse cryptochrome blue-light photoreceptor in circadian photoresponses. Science. 1998;282:1490-4 pubmed
    ..These data are consistent with the hypothesis that CRY2 protein modulates circadian responses in mice and suggest that cryptochromes have a role in circadian photoreception in mammals. ..
  4. Partch C, Clarkson M, Ozgur S, Lee A, Sancar A. Role of structural plasticity in signal transduction by the cryptochrome blue-light photoreceptor. Biochemistry. 2005;44:3795-805 pubmed
    ..Collectively, these findings provide the first biochemical evidence for the proposed conformational rearrangement of cryptochromes upon light exposure. ..
  5. Gauger M, Sancar A. Cryptochrome, circadian cycle, cell cycle checkpoints, and cancer. Cancer Res. 2005;65:6828-34 pubmed
    ..We conclude that the effect of circadian clock disruption on cellular response to DNA damage and cancer predisposition in mice may depend on the mechanism by which the clock is disrupted. ..
  6. Thompson C, Sancar A. Photolyase/cryptochrome blue-light photoreceptors use photon energy to repair DNA and reset the circadian clock. Oncogene. 2002;21:9043-56 pubmed
    ..Cryptochrome, which has a high degree of sequence identity to photolyase, works as the main circadian photoreceptor and as a component of the molecular clock in animals, including mammals, and regulates growth and development in plants. ..
  7. Kang T, Sancar A. Circadian regulation of DNA excision repair: implications for chrono-chemotherapy. Cell Cycle. 2009;8:1665-7 pubmed
    ..Here we review the significance of the connection linking the circadian clock with nucleotide excision repair and discuss potential implications for chemotherapy. ..
  8. Thompson C, Bowes Rickman C, Shaw S, Ebright J, Kelly U, Sancar A, et al. Expression of the blue-light receptor cryptochrome in the human retina. Invest Ophthalmol Vis Sci. 2003;44:4515-21 pubmed
  9. Thompson C, Selby C, Partch C, Plante D, Thresher R, Araujo F, et al. Further evidence for the role of cryptochromes in retinohypothalamic photoreception/phototransduction. Brain Res Mol Brain Res. 2004;122:158-66 pubmed
  10. Thompson C, Selby C, Van Gelder R, Blaner W, Lee J, Quadro L, et al. Effect of vitamin A depletion on nonvisual phototransduction pathways in cryptochromeless mice. J Biol Rhythms. 2004;19:504-17 pubmed
    ..These data demonstrate that both cryptochromes and opsins regulate nonvisual photoresponses. ..
  11. Partch C, Shields K, Thompson C, Selby C, Sancar A. Posttranslational regulation of the mammalian circadian clock by cryptochrome and protein phosphatase 5. Proc Natl Acad Sci U S A. 2006;103:10467-10472 pubmed publisher
    ..Collectively, these findings indicate that PP5, CKIepsilon, and cryptochrome dynamically regulate the mammalian circadian clock. ..
  12. Miyamoto Y, Sancar A. Circadian regulation of cryptochrome genes in the mouse. Brain Res Mol Brain Res. 1999;71:238-43 pubmed
    ..Third, mutation in Cry2 causes a phase delay in Cry1 and mPer1 expression both in the SCN and internal organs relative to wild-type animals. Finally, no obvious periodicity in mCry2 expression was seen in all tissues tested. ..
  13. Miyamoto Y, Sancar A. Vitamin B2-based blue-light photoreceptors in the retinohypothalamic tract as the photoactive pigments for setting the circadian clock in mammals. Proc Natl Acad Sci U S A. 1998;95:6097-102 pubmed
  14. Hsu D, Zhao X, Zhao S, Kazantsev A, Wang R, Todo T, et al. Putative human blue-light photoreceptors hCRY1 and hCRY2 are flavoproteins. Biochemistry. 1996;35:13871-7 pubmed
    ..We conclude that these newly discovered members of the photolyase/photoreceptor family are not photolyases and instead may function as blue-light photoreceptors in humans. ..
  15. Unsal KaƧmaz K, Mullen T, Kaufmann W, Sancar A. Coupling of human circadian and cell cycles by the timeless protein. Mol Cell Biol. 2005;25:3109-16 pubmed
  16. Ozgur S, Sancar A. Analysis of autophosphorylating kinase activities of Arabidopsis and human cryptochromes. Biochemistry. 2006;45:13369-74 pubmed
    ..Finally, we find that the kinase activity of AtCry1 is not significantly affected by light or the redox status of the flavin cofactor. ..