Genomes and Genes
Experts and Doctors on drosophila in Durham, North Carolina, United States
Locale: Durham, North Carolina, United States
- Keene J. RNA regulons: coordination of post-transcriptional events. Nat Rev Genet. 2007;8:533-43 pubmed..I close by considering the evolutionary wiring and rewiring of these combinatorial post-transcriptional gene-expression networks. ..
- Peralta X, Toyama Y, Hutson M, Montague R, Venakides S, Kiehart D, et al. Upregulation of forces and morphogenic asymmetries in dorsal closure during Drosophila development. Biophys J. 2007;92:2583-96 pubmed..Asymmetries in these mutants are reversed compared to wild-type, and inhomogeneous stresses may cause asymmetries in wild-type closure. ..
- Megosh H, Cox D, Campbell C, Lin H. The role of PIWI and the miRNA machinery in Drosophila germline determination. Curr Biol. 2006;16:1884-94 pubmed
- Laurie C, Heath E, Jacobson J, Thomson M. Genetic basis of the difference in alcohol dehydrogenase expression between Drosophila melanogaster and Drosophila simulans. Proc Natl Acad Sci U S A. 1990;87:9674-8 pubmed..Therefore, the interspecific difference in Adh expression is due entirely to trans-acting modifiers, in spite of the many sequence differences between the Adh genes of the two species, which include two amino acid substitutions. ..
- Freel C, Richardson D, Thomenius M, Gan E, Horn S, Olson M, et al. Mitochondrial localization of Reaper to promote inhibitors of apoptosis protein degradation conferred by GH3 domain-lipid interactions. J Biol Chem. 2008;283:367-79 pubmed
- Kim I, Wolf M. Serial examination of an inducible and reversible dilated cardiomyopathy in individual adult Drosophila. PLoS ONE. 2009;4:e7132 pubmed publisher..Furthermore, the induction and reversal of cardiac transgene expression can be investigated in adult flies thereby providing insight into the post-developmental effects of transgene expression. ..
- Hsieh T, Capp C. Nucleotide- and stoichiometry-dependent DNA supercoiling by reverse gyrase. J Biol Chem. 2005;280:20467-75 pubmed..The possible mechanistic insights from these findings will be presented here. ..
- Wang Z, Lin H. The division of Drosophila germline stem cells and their precursors requires a specific cyclin. Curr Biol. 2005;15:328-33 pubmed..Our results indicate that stem cells may use specific cell cycle regulators for their division. ..
- Jones W, Bejsovec A. Wingless signaling: an axin to grind. Curr Biol. 2003;13:R479-81 pubmed..New findings in Drosophila reveal a novel mechanism for down-regulating the activity of the Wingless/Wnt pathway. ..
- Bloor J, Kiehart D. Drosophila RhoA regulates the cytoskeleton and cell-cell adhesion in the developing epidermis. Development. 2002;129:3173-83 pubmed..At the leading edge, cells show altered adhesive properties such that they form ectopic contacts with other DRhoA(N19)-expressing cells. ..
- Heil C, Noor M. Zinc finger binding motifs do not explain recombination rate variation within or between species of Drosophila. PLoS ONE. 2012;7:e45055 pubmed publisher..We suggest these findings could be explained by the existence of a different recombination initiation system in Drosophila. ..
- Capel B. The battle of the sexes. Mech Dev. 2000;92:89-103 pubmed
- Bogerd H, Whisnant A, Kennedy E, Flores O, Cullen B. Derivation and characterization of Dicer- and microRNA-deficient human cells. RNA. 2014;20:923-37 pubmed publisher..passenger strands for a subset of human miRNAs. These data demonstrate that the mechanisms underlying the accurate processing of pre-miRNAs are largely, but not entirely, conserved between mammalian and insect cells...
- Liu J, Mercer J, Stam L, Gibson G, Zeng Z, Laurie C. Genetic analysis of a morphological shape difference in the male genitalia of Drosophila simulans and D. mauritiana. Genetics. 1996;142:1129-45 pubmed..Although there is some evidence of partial dominance of mauritiana alleles and for epistasis, the pattern of inheritance is largely additive...
- True J, Mercer J, Laurie C. Differences in crossover frequency and distribution among three sibling species of Drosophila. Genetics. 1996;142:507-23 pubmed..In particular, mauritiana is expected to differ from melanogaster in patterns and amounts of sequence variation and transposon insertions. ..
- Endow S, Komma D. Centrosome and spindle function of the Drosophila Ncd microtubule motor visualized in live embryos using Ncd-GFP fusion proteins. J Cell Sci. 1996;109 ( Pt 10):2429-42 pubmed..This implies that the Ncd motor functions to prevent chromosome loss by maintaining chromosome attachment to the spindle in metaphase, consistent with the Ncd-GFP fibers that across the metaphase plate. ..
- Komma D, Endow S. Enhancement of the ncdD microtubule motor mutant by mutants of alpha Tub67C. J Cell Sci. 1997;110 ( Pt 2):229-37 pubmed..These results imply that a specific alpha-tubulin isoform is required for normal cellular function of a kinesin motor protein...
- Erickson H. Tenascin-C, tenascin-R and tenascin-X: a family of talented proteins in search of functions. Curr Opin Cell Biol. 1993;5:869-76 pubmed..Moreover, two new proteins have been discovered in the last year that are closely related to the original tenascin, providing evidence for a tenascin family. Speculations on functions are ripe for re-evaluation. ..
- Araki M, Yu H, Asano M. A novel motif governs APC-dependent degradation of Drosophila ORC1 in vivo. Genes Dev. 2005;19:2458-65 pubmed
- Song H, Endow S. Binding sites on microtubules of kinesin motors of the same or opposite polarity. Biochemistry. 1996;35:11203-9 pubmed..These differences in binding sites may be related to the opposite polarity of translocation on MTs of the motors. ..
- Wang Z, Lin H. Nanos maintains germline stem cell self-renewal by preventing differentiation. Science. 2004;303:2016-9 pubmed..Thus, Nanos is essential for both establishing and maintaining GSCs by preventing their precocious entry into oogenesis. These functions are likely achieved by repressing the translation of differentiation factors in PGCs and GSCs. ..
- Dahanukar A, Wharton R. The Nanos gradient in Drosophila embryos is generated by translational regulation. Genes Dev. 1996;10:2610-20 pubmed..This coupling suggests a model in which the polarized distribution of nos protein is generated primarily by translational control and that nos mRNA localization is a byproduct of this regulation, at least in part. ..
- Bennett S, Noor M. Molecular evolution of a Drosophila homolog of human BRCA2. Genetica. 2009;137:213-9 pubmed publisher..Overall, we have documented patterns and modes of evolution in a new model system of a gene which is important to human health...
- Lee J, Koga H, Kawaguchi Y, Tang W, Wong E, Gao Y, et al. HDAC6 controls autophagosome maturation essential for ubiquitin-selective quality-control autophagy. EMBO J. 2010;29:969-80 pubmed publisher..Our study identifies HDAC6 and the actin cytoskeleton as critical components that define QC autophagy and uncovers a novel regulation of autophagy at the level of autophagosome-lysosome fusion. ..
- Liu H, Hallen M, Endow S. Altered nucleotide-microtubule coupling and increased mechanical output by a kinesin mutant. PLoS ONE. 2012;7:e47148 pubmed publisher..One possibility, based on our findings, is that movements by the residue and the loop that contains it could bend or distort the central ?-sheet, mediating free energy changes that lead to force production. ..
- Hallen M, Endow S. Anastral spindle assembly: a mathematical model. Biophys J. 2009;97:2191-201 pubmed publisher..These findings are consistent with spindle assembly in live wild-type and ncd mutant Drosophila oocytes. ..
- Zhang C, Rothwell W, Sullivan W, Hsieh T. Discontinuous actin hexagon, a protein essential for cortical furrow formation in Drosophila, is membrane associated and hyperphosphorylated. Mol Biol Cell. 2000;11:1011-22 pubmed..Both DAH localization and phosphorylation are disrupted in another maternal-effect mutant, nuclear-fallout. It is possible that nuclear-fallout collaborates with dah and directs DAH protein localization to the cortical furrows. ..
- Kandul N, Wright K, Kandul E, Noor M. No evidence for learned mating discrimination in male Drosophila pseudoobscura. BMC Evol Biol. 2006;6:54 pubmed..males that had been maintained in isolation. We conclude that learning to avoid heterospecific courtship may not be universal, even within the genus Drosophila, and may possibly be dependent on the natural history of the species. ..
- Sonoda J, Wharton R. Drosophila Brain Tumor is a translational repressor. Genes Dev. 2001;15:762-73 pubmed..The results of these experiments suggest that NHL domain proteins are recruited to various mRNAs by combinatorial protein-protein interactions. ..
- Lamb R, Ward R, Schweizer L, Fehon R. Drosophila coracle, a member of the protein 4.1 superfamily, has essential structural functions in the septate junctions and developmental functions in embryonic and adult epithelial cells. Mol Biol Cell. 1998;9:3505-19 pubmed..1 superfamily members. These studies provide insights into a range of in vivo functions for coracle in developing embryos and adults. ..
- Hutson M, Tokutake Y, Chang M, Bloor J, Venakides S, Kiehart D, et al. Forces for morphogenesis investigated with laser microsurgery and quantitative modeling. Science. 2003;300:145-9 pubmed
- Hallen M, Liang Z, Endow S. Ncd motor binding and transport in the spindle. J Cell Sci. 2008;121:3834-41 pubmed publisher..Minus-end Ncd thus binds tightly to spindles and is transported in early metaphase towards microtubule plus-ends, the opposite direction to that in which the motor moves, to produce force in the spindle later in mitosis. ..
- Wilson Sali T, Hsieh T. Preferential cleavage of plasmid-based R-loops and D-loops by Drosophila topoisomerase IIIbeta. Proc Natl Acad Sci U S A. 2002;99:7974-9 pubmed..These studies suggest that the topo III enzyme behaves as a structure-specific endonuclease in vivo, providing a reversible DNA cleavage activity that is specific for unpaired regions in the DNA. ..
- Araki M, Wharton R, Tang Z, Yu H, Asano M. Degradation of origin recognition complex large subunit by the anaphase-promoting complex in Drosophila. EMBO J. 2003;22:6115-26 pubmed..These observations suggest that in Drosophila, ORC1 regulates origin utilization much as does Cdc6 in budding yeast. ..
- Park S, Asano M. The origin recognition complex is dispensable for endoreplication in Drosophila. Proc Natl Acad Sci U S A. 2008;105:12343-8 pubmed publisher..This study provides insight into the physiologic roles of ORC during metazoan development and proposes that DNA replication initiation is governed differently in mitotic and endocycles. ..
- Casad M, Yu L, Daniels J, Wolf M, Rockman H. Deletion of Siah-interacting protein gene in Drosophila causes cardiomyopathy. Mol Genet Genomics. 2012;287:351-60 pubmed publisher..Alterations in Armadillo signaling during development lead to important changes in the size and function of the adult heart. ..
- Fehon R, Dawson I, Artavanis Tsakonas S. A Drosophila homologue of membrane-skeleton protein 4.1 is associated with septate junctions and is encoded by the coracle gene. Development. 1994;120:545-57 pubmed..These data indicate that D4.1 is associated with the septate junction, and suggest that it may play a role in cell-cell interactions that are essential for normal development. ..
- Brown S, Zhang C, Chen A, Hsieh T. Structure of the Drosophila DNA topoisomerase I gene and expression of messages with different lengths in the 3' untranslated region. Gene. 1998;211:195-203 pubmed..No significant heterogeneity at the 5' end of the top1 transcripts is seen. Sequence searches have revealed a number of regulatory sequences for potential translational control in the 3' untranslated region. ..
- Lin H, Schagat T. Neuroblasts: a model for the asymmetric division of stem cells. Trends Genet. 1997;13:33-9 pubmed..These findings are illuminating in understanding the general mechanism of stem cell division. ..
- Weeks J, Hardin S, Shen J, Lee J, Greenleaf A. Locus-specific variation in phosphorylation state of RNA polymerase II in vivo: correlations with gene activity and transcript processing. Genes Dev. 1993;7:2329-44 pubmed..Finally, using antibodies to hnRNP proteins and splicing components, we have discerned an apparent overall correlation between the presence and processing of nascent transcripts and the presence of Pol IIO. ..
- Kim B, Turski M, Nose Y, Casad M, Rockman H, Thiele D. Cardiac copper deficiency activates a systemic signaling mechanism that communicates with the copper acquisition and storage organs. Cell Metab. 2010;11:353-63 pubmed publisher..These studies identify ATP7A as a candidate for hepatic Cu mobilization in response to peripheral tissue demand, and illuminate a systemic regulation in which the Cu status of the heart is signaled to organs that take up and store Cu. ..
- Walton K, Warner J, Hertzler P, McClay D. Hedgehog signaling patterns mesoderm in the sea urchin. Dev Biol. 2009;331:26-37 pubmed publisher..Together the data support the requirement of Hh signaling in patterning each of the mesoderm subtypes in the sea urchin embryo. ..
- Ward R, Schweizer L, Lamb R, Fehon R. The protein 4.1, ezrin, radixin, moesin (FERM) domain of Drosophila Coracle, a cytoplasmic component of the septate junction, provides functions essential for embryonic development and imaginal cell proliferation. Genetics. 2001;159:219-28 pubmed..This result suggests the presence of a second functional domain within Coracle, a notion supported by molecular characterization of a series of coracle alleles. ..
- Parisi M, Deng W, Wang Z, Lin H. The arrest gene is required for germline cyst formation during Drosophila oogenesis. Genesis. 2001;29:196-209 pubmed..These defects of aret mutants reveal a novel function of aret as the first gene with a defined function in the cystoblast to cyst transition during early oogenesis. ..
- Genova J, Jong S, Camp J, Fehon R. Functional analysis of Cdc42 in actin filament assembly, epithelial morphogenesis, and cell signaling during Drosophila development. Dev Biol. 2000;221:181-94 pubmed..These results confirm the role of Cdc42 in actin filament assembly and provide new insights into its functions in epithelial morphogenesis and regulating intercellular signaling events. ..
- Murata Y, Wharton R. Binding of pumilio to maternal hunchback mRNA is required for posterior patterning in Drosophila embryos. Cell. 1995;80:747-56 pubmed..These and other observations suggest that pum acts by recognizing the NRE and then recruiting nos. Presumably, the resulting complex inhibits some component of the translation machinery. ..
- Kandul N, Noor M. Large introns in relation to alternative splicing and gene evolution: a case study of Drosophila bruno-3. BMC Genet. 2009;10:67 pubmed publisher..Large introns could be a reservoir of genetic diversity, because they have a greater number of mutable sites than short introns. Taken together, gene structure can constrain and/or promote gene evolution...
- Lee M, Hsieh T. Incomplete reversion of double stranded DNA cleavage mediated by Drosophila topoisomerase II: formation of single stranded DNA cleavage complex in the presence of an anti-tumor drug VM26. Nucleic Acids Res. 1992;20:5027-33 pubmed..We discuss the possible significance of this finding to the mechanism of action of VM26 in the topoisomerase II reactions. ..
- Thorne N, Bray S, Amrein H. Function and expression of the Drosophila gr genes in the perception of sweet, bitter and pheromone compounds. Chem Senses. 2005;30 Suppl 1:i270-2 pubmed
- Szakmary A, Cox D, Wang Z, Lin H. Regulatory relationship among piwi, pumilio, and bag-of-marbles in Drosophila germline stem cell self-renewal and differentiation. Curr Biol. 2005;15:171-8 pubmed
- Endow S, Komma D. Spindle dynamics during meiosis in Drosophila oocytes. J Cell Biol. 1997;137:1321-36 pubmed..The genesis of spindle defects in the live mutant oocytes provides new insights into the mechanism of Ncd function in the spindle during the meiotic divisions. ..
- Wilson Sali T, Hsieh T. Generation of double-stranded breaks in hypernegatively supercoiled DNA by Drosophila topoisomerase IIIbeta, a type IA enzyme. J Biol Chem. 2002;277:26865-71 pubmed..These results suggest that topo IIIbeta may function as a reversible endonuclease in vivo by recognizing and cleaving/rejoining DNA structures with single-stranded character. ..
- Fujii S, Krishnan P, Hardin P, Amrein H. Nocturnal male sex drive in Drosophila. Curr Biol. 2007;17:244-51 pubmed
- Hoehn K, McGaugh S, Noor M. Effects of premature termination codon polymorphisms in the Drosophila pseudoobscura subclade. J Mol Evol. 2012;75:141-50 pubmed publisher..Ultimately, these analyzes support the view that the PTC mutations are mostly detrimental, but are nonetheless common enough in genomes that a subset could be effectively neutral...
- Hallen M, Ho J, Yankel C, Endow S. Fluorescence recovery kinetic analysis of gamma-tubulin binding to the mitotic spindle. Biophys J. 2008;95:3048-58 pubmed publisher..gamma-Tubulin may interact transiently with the spindle, nucleating microtubules very rapidly, differing from centrosomes, where gamma-tubulin binds tightly to nucleate microtubules. ..
- Chandra R, Salmon E, Erickson H, Lockhart A, Endow S. Structural and functional domains of the Drosophila ncd microtubule motor protein. J Biol Chem. 1993;268:9005-13 pubmed..Physical properties of the C terminus proteins indicate that the stalk region is important for dimerization and that the ncd protein probably exists and functions as a dimer. ..
- Edwards K, Demsky M, Montague R, Weymouth N, Kiehart D. GFP-moesin illuminates actin cytoskeleton dynamics in living tissue and demonstrates cell shape changes during morphogenesis in Drosophila. Dev Biol. 1997;191:103-17 pubmed
- McGaugh S, Heil C, Manzano Winkler B, Loewe L, Goldstein S, Himmel T, et al. Recombination modulates how selection affects linked sites in Drosophila. PLoS Biol. 2012;10:e1001422 pubmed publisher..Finally, we find evidence for dips in diversity around nonsynonymous substitutions. We infer that at least some of this reduction in diversity resulted from selective sweeps and examine these dips in the context of recombination rate. ..
- Smulders Srinivasan T, Lin H. Screens for piwi suppressors in Drosophila identify dosage-dependent regulators of germline stem cell division. Genetics. 2003;165:1971-91 pubmed..The other two types are deficiencies that cause lethality and female-specific lethality in a piwi2 mutant background, revealing the zygotic function of piwi in somatic development. ..
- Chang S, Hu T, Hsieh T. Analysis of a core domain in Drosophila DNA topoisomerase II. Targeting of an antitumor agent ICRF-159. J Biol Chem. 1998;273:19822-8 pubmed..Furthermore, the salt stability of the closed protein clamp induced by ICRF-159 depends on the presence and closure of the N-terminal ATPase domain. ..
- King F, Lin H. Somatic signaling mediated by fs(1)Yb is essential for germline stem cell maintenance during Drosophila oogenesis. Development. 1999;126:1833-44 pubmed
- Sonoda J, Wharton R. Recruitment of Nanos to hunchback mRNA by Pumilio. Genes Dev. 1999;13:2704-12 pubmed..Taken together, these findings suggest models for how Nos and Pum collaboratively target hb mRNA. More generally, they suggest that Pum-like proteins from other species may also act by recruiting cofactors to regulate translation. ..
- Sloan R, Swanson C, Gavilano L, Smith K, Malek P, Snow Smith M, et al. Characterization of null and hypomorphic alleles of the Drosophila l(2)dtl/cdt2 gene: Larval lethality and male fertility. Fly (Austin). 2012;6:173-83 pubmed publisher..We conclude that "denticleless" is a misnomer, but that l(2)dtl/cdt2 is an essential gene for Drosophila development. ..
- Lee M, Sander M, Hsieh T. Single strand DNA cleavage reaction of duplex DNA by Drosophila topoisomerase II. J Biol Chem. 1989;264:13510-8 pubmed..The implication of this observation on the sequential cleavage of the two strands of the DNA duplex during the normal DNA double strand passage process catalyzed by type II DNA topoisomerases is discussed. ..
- LaJeunesse D, McCartney B, Fehon R. Structural analysis of Drosophila merlin reveals functional domains important for growth control and subcellular localization. J Cell Biol. 1998;141:1589-99 pubmed..These results provide insights into the functions and functional organization of a novel tumor suppressor gene. ..
- Ward R, Lamb R, Fehon R. A conserved functional domain of Drosophila coracle is required for localization at the septate junction and has membrane-organizing activity. J Cell Biol. 1998;140:1463-73 pubmed..1 superfamily members. ..
- Parisi M, Lin H. The Drosophila pumilio gene encodes two functional protein isoforms that play multiple roles in germline development, gonadogenesis, oogenesis and embryogenesis. Genetics. 1999;153:235-50 pubmed..Finally, we present molecular evidence suggesting that the two PUM isoforms share some of their primary structures. ..
- Bloor J, Kiehart D. zipper Nonmuscle myosin-II functions downstream of PS2 integrin in Drosophila myogenesis and is necessary for myofibril formation. Dev Biol. 2001;239:215-28 pubmed publisher..We suggest that nonmuscle myosin-II functions at the muscle termini and the Z-line as an actin crosslinker and acts to maintain the structural integrity of the sarcomere...
- Olson M, Holley C, Yoo S, Huh J, Hay B, Kornbluth S. Reaper is regulated by IAP-mediated ubiquitination. J Biol Chem. 2003;278:4028-34 pubmed..These data demonstrate a novel function for IAPs and suggest that IAPs and Reaper-like proteins mutually control each other's abundance. ..
- True J, Weir B, Laurie C. A genome-wide survey of hybrid incompatibility factors by the introgression of marked segments of Drosophila mauritiana chromosomes into Drosophila simulans. Genetics. 1996;142:819-37 pubmed
- Bennett S, Mercer J, Noor M. Slip-sliding away: serial changes and homoplasy in repeat number in the Drosophila yakuba homolog of human cancer susceptibility gene BRCA2. PLoS ONE. 2010;5:e11006 pubmed publisher..We conclude that the evolutionary history of dmbrca2 in D. yakuba and perhaps in other Drosophila species may be more complicated than can be inferred from examination of the published single genome sequences per species. ..
- Reedy M, Beall C, Fyrberg E. Formation of reverse rigor chevrons by myosin heads. Nature. 1989;339:481-3 pubmed
- Nijhout H. Genes on the wing. Science. 1994;265:44-5 pubmed
- King P, Levine T, Fremeau R, Keene J. Mammalian homologs of Drosophila ELAV localized to a neuronal subset can bind in vitro to the 3' UTR of mRNA encoding the Id transcriptional repressor. J Neurosci. 1994;14:1943-52 pubmed..These findings are interpreted in light of recent studies in which mRNA 3' UTRs were found to be important for the regulation of cell growth and differentiation. ..
- Endow S, Komma D. Assembly and dynamics of an anastral:astral spindle: the meiosis II spindle of Drosophila oocytes. J Cell Sci. 1998;111 ( Pt 17):2487-95 pubmed
- Skantar A, Greenleaf A. Identifying a transcription factor interaction site on RNA polymerase II. Gene Expr. 1995;5:49-69 pubmed..Finally, dTFIIF can be specifically cross-linked to a GST-IIc(A519-G992) fusion protein or to subunit IIc in intact Pol II. ..
- Verrotti A, Wharton R. Nanos interacts with cup in the female germline of Drosophila. Development. 2000;127:5225-32 pubmed..They further suggest that Nos and Pum are likely to collaborate during oogenesis, as they do during embryogenesis. ..
- Sköld H, Komma D, Endow S. Assembly pathway of the anastral Drosophila oocyte meiosis I spindle. J Cell Sci. 2005;118:1745-55 pubmed..This is the first complete account of assembly of an anastral spindle and the specific steps that require Ncd motor activity, revealing new and unexpected features of the process. ..