Genomes and Genes
Experts and Doctors on dna binding proteins in Chapel Hill, North Carolina, United States
Locale: Chapel Hill, North Carolina, United States
Topic: dna binding proteins
- Perkins E, Nair A, Cowley D, van Dyke T, Chang Y, Ramsden D. Sensing of intermediates in V(D)J recombination by ATM. Genes Dev. 2002;16:159-64 pubmed..We suggest routine surveillance of intermediates in V(D)J recombination by ATM helps suppress potentially oncogenic translocations when repair fails. ..
- Cole K, Harmon A, Harp J, Patel Y. Rb regulates C/EBPbeta-DNA-binding activity during 3T3-L1 adipogenesis. Am J Physiol Cell Physiol. 2004;286:C349-54 pubmed..We conclude that C/EBPbeta-DNA-binding activity is regulated by its interaction with hypophosphorylated Rb, thereby linking the progression of the cell cycle to the initiation of differentiation during 3T3-L1 adipogenesis. ..
- Cuevas B, Uhlik M, Garrington T, Johnson G. MEKK1 regulates the AP-1 dimer repertoire via control of JunB transcription and Fra-2 protein stability. Oncogene. 2005;24:801-9 pubmed..Controlling the repertoire of a transcription factor complex is a newly defined function for an MAPK kinase kinase. ..
- Kim J, Jobin C. The flavonoid luteolin prevents lipopolysaccharide-induced NF-kappaB signalling and gene expression by blocking IkappaB kinase activity in intestinal epithelial cells and bone-marrow derived dendritic cells. Immunology. 2005;115:375-87 pubmed..Modulation of innate immunity by natural plant products may represent an attractive strategy to prevent intestinal inflammation associated with dysregulated innate immune responses. ..
- Fiordalisi J, Johnson R, Weinbaum C, Sakabe K, Chen Z, Casey P, et al. High affinity for farnesyltransferase and alternative prenylation contribute individually to K-Ras4B resistance to farnesyltransferase inhibitors. J Biol Chem. 2003;278:41718-27 pubmed..Our results imply that relevant FTI targets will lack both polybasic and potentially geranylgeranylated methionine-CAAX motifs. ..
- Hu J, Zacharek S, He Y, Lee H, Shumway S, Duronio R, et al. WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase. Genes Dev. 2008;22:866-71 pubmed publisher..These results indicate that FBW5-DDB1-CUL4-ROC1 is an E3 ubiquitin ligase regulating TSC2 protein stability and TSC complex turnover. ..
- Waters J, Chen R, Murray A, Gorbsky G, Salmon E, Nicklas R. Mad2 binding by phosphorylated kinetochores links error detection and checkpoint action in mitosis. Curr Biol. 1999;9:649-52 pubmed..Thus, we have identified a probable molecular link between attachment-sensitive kinetochore phosphorylation and the inhibition of anaphase. The complete pathway for error control in mitosis can now be outlined. ..
- Griffith J, Lindsey Boltz L, Sancar A. Structures of the human Rad17-replication factor C and checkpoint Rad 9-1-1 complexes visualized by glycerol spray/low voltage microscopy. J Biol Chem. 2002;277:15233-6 pubmed..Therefore, our results demonstrate structural similarity between the checkpoint Rad complexes and the PCNA and RFC replication factors and thus provide further support for models proposing analogous functions for these complexes. ..
- Cao R, Wang H, He J, Erdjument Bromage H, Tempst P, Zhang Y. Role of hPHF1 in H3K27 methylation and Hox gene silencing. Mol Cell Biol. 2008;28:1862-72 pubmed..Therefore, our results reveal hPHF1 as a component of a novel EED-EZH2 complex and demonstrate its important role in H3K27 methylation and Hox gene silencing. ..
- Jobin C, Sartor R. The I kappa B/NF-kappa B system: a key determinant of mucosalinflammation and protection. Am J Physiol Cell Physiol. 2000;278:C451-62 pubmed..The key role of NF-kappa B in regulating expression of a number of proinflammatory genes makes this protein an attractive target for selective therapeutic intervention...
- Choi J, Lindsey Boltz L, Sancar A. Cooperative activation of the ATR checkpoint kinase by TopBP1 and damaged DNA. Nucleic Acids Res. 2009;37:1501-9 pubmed publisher..Taken together, these findings suggest a cooperative activation mechanism for the ATR checkpoint kinase by TopBP1 and damaged DNA. ..
- Reardon J, Sancar A. Molecular anatomy of the human excision nuclease assembled at sites of DNA damage. Mol Cell Biol. 2002;22:5938-45 pubmed..Based on these results, we propose that RPA70 makes the initial contact with psoralen-damaged DNA but that within preincision complexes, it is RPA32 and XPD that are in close contact with the lesion. ..
- Satoh M, Shaheen V, Kao P, Okano T, Shaw M, Yoshida H, et al. Autoantibodies define a family of proteins with conserved double-stranded RNA-binding domains as well as DNA binding activity. J Biol Chem. 1999;274:34598-604 pubmed..These autoantibodies will be useful probes of the function of dsRNA-binding proteins. Their interaction with dsRNA, an immunological adjuvant, also could promote autoimmunity. ..
- Bergstralh D, Sekelsky J. Interstrand crosslink repair: can XPF-ERCC1 be let off the hook?. Trends Genet. 2008;24:70-6 pubmed publisher..However, recent data has implicated XPF-ERCC1 in homologous recombination. We suggest that this role, rather than its function in nucleotide excision repair, defines its importance to ICL repair. ..
- Wang J, Mager J, Schnedier E, Magnuson T. The mouse PcG gene eed is required for Hox gene repression and extraembryonic development. Mamm Genome. 2002;13:493-503 pubmed..However, genetic crosses with a Mash2 null allele suggested that Eed was not required to maintain Mash2 imprinting, but could be required in a lineage specific fashion to suppress Mash2 expression. ..
- Tsukada S, Westwick J, Ikejima K, Sato N, Rippe R. SMAD and p38 MAPK signaling pathways independently regulate alpha1(I) collagen gene expression in unstimulated and transforming growth factor-beta-stimulated hepatic stellate cells. J Biol Chem. 2005;280:10055-64 pubmed..In conclusion, both p38 MAPK and Smad signaling independently and additively regulate alpha1(I) collagen gene expression by transcriptional activation, whereas p38 MAPK and not Smad signaling increased alpha1(I) collagen mRNA stability. ..
- Li C, Samulski R. Serotype-specific replicating AAV helper constructs increase recombinant AAV type 2 vector production. Virology. 2005;335:10-21 pubmed..This strategy supports development of AAV packaging systems that retain normal virus replication capability without helper virus encapsidation...
- Choi J, Sancar A, Lindsey Boltz L. The human ATR-mediated DNA damage checkpoint in a reconstituted system. Methods. 2009;48:3-7 pubmed publisher..This system provides a powerful tool to gain insight into the molecular mechanism of the ATR pathway. Here we describe preparation of the checkpoint components and our specific kinase assay in more detail. ..
- Cade R, Errede B. MOT2 encodes a negative regulator of gene expression that affects basal expression of pheromone-responsive genes in Saccharomyces cerevisiae. Mol Cell Biol. 1994;14:3139-49 pubmed..Because mot2 deletion mutants also have a conditional cell lysis phenotype, we expect that Mot2p regulatory effects may be more global than for mating-specific gene expression. ..
- Lee A, Wigle T, Singleton S. A complementary pair of rapid molecular screening assays for RecA activities. Anal Biochem. 2007;367:247-58 pubmed
- Park C, Sancar A. Formation of a ternary complex by human XPA, ERCC1, and ERCC4(XPF) excision repair proteins. Proc Natl Acad Sci U S A. 1994;91:5017-21 pubmed..We conclude that the XPA damage recognition protein makes a ternary complex with the ERCC1/ERCC4(XPF) heterodimer with a potential nuclease function. ..
- Li Y, Sancar A. Active site of Escherichia coli DNA photolyase: mutations at Trp277 alter the selectivity of the enzyme without affecting the quantum yield of photorepair. Biochemistry. 1990;29:5698-706 pubmed
- Schultz T, Quatrano R. Characterization and expression of a rice RAD23 gene. Plant Mol Biol. 1997;34:557-62 pubmed..A single transcript (1.5 kb) of osRAD23 was detected in total RNA from rice embryonic tissue, while three transcripts (1.8, 1.5 and 1.0 kb) were observed in total RNA from vegetative tissues of rice. ..
- Thrower D, Stemple J, Yeh E, Bloom K. Nuclear oscillations and nuclear filament formation accompany single-strand annealing repair of a dicentric chromosome in Saccharomyces cerevisiae. J Cell Sci. 2003;116:561-9 pubmed..Our findings indicate that microtubule-dependent processes facilitate recombination...
- Wu S, Zhang Y. Active DNA demethylation: many roads lead to Rome. Nat Rev Mol Cell Biol. 2010;11:607-20 pubmed publisher..Insight into how DNA methylation is dynamically regulated will broaden our understanding of epigenetic regulation and have great implications in somatic cell reprogramming and regenerative medicine. ..
- Selby C, Sancar A. Structure and function of transcription-repair coupling factor. II. Catalytic properties. J Biol Chem. 1995;270:4890-5 pubmed..In vitro, strand-specific repair is not dependent on the MutL and MutS proteins which have recently been implicated in preferential repair in vivo. ..
- Yancey Wrona J, Wood E, George J, Smith K, Matson S. Escherichia coli Rep protein and helicase IV. Distributive single-stranded DNA-dependent ATPases that catalyze a limited unwinding reaction in vitro. Eur J Biochem. 1992;207:479-85 pubmed..Taken together these data suggest that, in vitro, and in the absence of additional proteins, neither Rep protein nor helicase IV catalyzes a processive unwinding reaction. ..
- Cogswell P, Kashatus D, Keifer J, Guttridge D, Reuther J, Bristow C, et al. NF-kappa B and I kappa B alpha are found in the mitochondria. Evidence for regulation of mitochondrial gene expression by NF-kappa B. J Biol Chem. 2003;278:2963-8 pubmed..These data indicate that the NF-kappa B regulatory pathway exists in mitochondria and that NF-kappa B can negatively regulate mitochondrial mRNA expression. ..
- Sacho E, Kadyrov F, Modrich P, Kunkel T, Erie D. Direct visualization of asymmetric adenine-nucleotide-induced conformational changes in MutL alpha. Mol Cell. 2008;29:112-21 pubmed publisher..These data reveal an ATPase cycle in which sequential nucleotide binding, hydrolysis, and release modulate the conformational states of MutL alpha. ..
- Subramanian D, Griffith J. Interactions between p53, hMSH2-hMSH6 and HMG I(Y) on Holliday junctions and bulged bases. Nucleic Acids Res. 2002;30:2427-34 pubmed..Together, these results demonstrate that p53 DNA binding activity is highly influenced by the presence of other proteins, some having a dominant effect while others have a negative effect. ..
- Gates J, Mahaffey J, Rogers S, Emerson M, Rogers E, Sottile S, et al. Enabled plays key roles in embryonic epithelial morphogenesis in Drosophila. Development. 2007;134:2027-39 pubmed..We also explored filopodial regulation in cultured Drosophila cells and embryos. These data provide new insights into developmental and mechanistic roles of this important actin regulator. ..
- Cayirlioglu P, Bonnette P, Dickson M, Duronio R. Drosophila E2f2 promotes the conversion from genomic DNA replication to gene amplification in ovarian follicle cells. Development. 2001;128:5085-98 pubmed..These data indicate that E2f2 functions to inhibit widespread genomic DNA synthesis in late stage follicle cells, and may do so by repressing the expression of specific components of the replication machinery. ..
- Mohlke K, Boehnke M. The role of HNF4A variants in the risk of type 2 diabetes. Curr Diab Rep. 2005;5:149-56 pubmed..Rare loss-of-function mutations in HNF4A cause maturity-onset diabetes of the young and now common noncoding variants have been found to be associated with T2DM. ..
- Reardon J, Sancar A. Purification and characterization of Escherichia coli and human nucleotide excision repair enzyme systems. Methods Enzymol. 2006;408:189-213 pubmed..This chapter outlines methods for expression and purification of these essential repair factors and provides protocols for performing each of the in vitro repair assays with either the E. coli or the human excision nuclease. ..
- Zhou C, Gehrig P, Whang Y, Boggess J. Rapamycin inhibits telomerase activity by decreasing the hTERT mRNA level in endometrial cancer cells. Mol Cancer Ther. 2003;2:789-95 pubmed..This work suggests that rapamycin is a potentially useful targeted therapy for endometrial cancer and that loss of telomerase activity may be a good surrogate biomarker for assessing antitumor activity of rapamycin. ..
- Yang G, Zhang Y, Buchold G, Jetten A, O Brien D. Analysis of germ cell nuclear factor transcripts and protein expression during spermatogenesis. Biol Reprod. 2003;68:1620-30 pubmed..This expression profile suggests that GCNF plays a role in transcriptional regulation during meiosis and the early haploid phase of spermatogenesis. ..
- Mack C, Hinson J. Regulation of smooth muscle differentiation by the myocardin family of serum response factor co-factors. J Thromb Haemost. 2005;3:1976-84 pubmed
- Roberts S, Ramsden D. Loading of the nonhomologous end joining factor, Ku, on protein-occluded DNA ends. J Biol Chem. 2007;282:10605-13 pubmed..We suggest a model where Ku utilizes an unusual characteristic of its three-dimensional structure to recognize certain protein-occluded ends without the extensive remodeling of chromatin structure required by other DNA repair pathways. ..
- Zhang H, Wang L, Kao S, Whitehead I, Hart M, Liu B, et al. Functional interaction between the cytoplasmic leucine-zipper domain of HIV-1 gp41 and p115-RhoGEF. Curr Biol. 1999;9:1271-4 pubmed..These findings suggest that an important function of gp41C is to modulate the activity of p115-RhoGEF and they thus reveal a new potential anti-HIV-1 target. ..
- Hummer B, Bartlett C, Henry E, Weissman B. Expression of Smad4 in the FaDu cell line partially restores TGF-beta growth inhibition but is not sufficient to regulate fibronectin expression or suppress tumorigenicity. J Cell Physiol. 2003;194:289-302 pubmed..They also demonstrate the advantages of providing a physiological extracellular environment, when assessing TGFbeta responsiveness. ..
- Thresher R, Makhov A, Hall S, Kolodner R, Griffith J. Electron microscopic visualization of RecT protein and its complexes with DNA. J Mol Biol. 1995;254:364-71 pubmed
- Jiang L, Pearson J, Crews S. Diverse modes of Drosophila tracheal fusion cell transcriptional regulation. Mech Dev. 2010;127:265-80 pubmed publisher..These results begin to decode the regulatory circuitry that guides transcriptional activation of genes required for fusion cell morphogenesis. ..
- Ritchie K, Mallory J, Petes T. Interactions of TLC1 (which encodes the RNA subunit of telomerase), TEL1, and MEC1 in regulating telomere length in the yeast Saccharomyces cerevisiae. Mol Cell Biol. 1999;19:6065-75 pubmed..This result argues that the role of Tel1p in telomere maintenance is not solely a direct activation of telomerase. ..
- Hara R, Selby C, Liu M, Price D, Sancar A. Human transcription release factor 2 dissociates RNA polymerases I and II stalled at a cyclobutane thymine dimer. J Biol Chem. 1999;274:24779-86 pubmed
- Mo J, Xia Y, Ning Z, Wade T, Mumford J. Elevated ERCC1 gene expression in blood cells associated with exposure to arsenic from drinking water in Inner Mongolia. Anticancer Res. 2009;29:3253-9 pubmed..275, p<0.0001). The results showed that mRNA levels of ERCC1 expression were significantly associated with arsenic concentrations in drinking water, implicating the DNA repair response was induced by arsenic exposure. ..
- Barros S, Offenbacher S. Epigenetics: connecting environment and genotype to phenotype and disease. J Dent Res. 2009;88:400-8 pubmed publisher..The challenge in oral biology will be to understand the mechanisms that modify MVPs in oral tissues and to identify those epigenetic patterns that modify disease pathogenesis or responses to therapy. ..
- Dominski Z. Nucleases of the metallo-beta-lactamase family and their role in DNA and RNA metabolism. Crit Rev Biochem Mol Biol. 2007;42:67-93 pubmed..This article reviews the cellular roles of nucleases of the metallo-beta-lactamase family and the recent advances in studying these proteins. ..
- Wang Y, Dohlman H. Pheromone-regulated sumoylation of transcription factors that mediate the invasive to mating developmental switch in yeast. J Biol Chem. 2006;281:1964-9 pubmed..In the absence of sumoylation Tec1 is more rapidly degraded. We propose that pheromone-regulated sumoylation of Ste12 and Tec1 promotes a developmental switch from the invasive to the mating differentiation program. ..
- Laribee R, Krogan N, Xiao T, Shibata Y, Hughes T, Greenblatt J, et al. BUR kinase selectively regulates H3 K4 trimethylation and H2B ubiquitylation through recruitment of the PAF elongation complex. Curr Biol. 2005;15:1487-93 pubmed..Our data reveal a novel function for the BUR kinase in transcriptional regulation through the selective control of histone modifications. ..
- To J, Haberer G, Ferreira F, Deruère J, Mason M, Schaller G, et al. Type-A Arabidopsis response regulators are partially redundant negative regulators of cytokinin signaling. Plant Cell. 2004;16:658-71 pubmed..Further, morphological phenotypes of some arr mutants suggest complex regulatory interactions and gene-specific functions among family members. ..
- Baldwin A. The NF-kappa B and I kappa B proteins: new discoveries and insights. Annu Rev Immunol. 1996;14:649-83 pubmed
- Stevens T, Rogers E, Koontz L, Fox D, Homem C, Nowotarski S, et al. Using Bcr-Abl to examine mechanisms by which abl kinase regulates morphogenesis in Drosophila. Mol Biol Cell. 2008;19:378-93 pubmed..These data provide new insight into how regulated Abl activity helps direct normal development and into possible biological functions of Bcr-Abl. ..
- Kearney H, Kirkpatrick D, Gerton J, Petes T. Meiotic recombination involving heterozygous large insertions in Saccharomyces cerevisiae: formation and repair of large, unpaired DNA loops. Genetics. 2001;158:1457-76 pubmed..Although meiotic recombination events in yeast are initiated by double-strand DNA breaks (DSBs), we showed that DSBs occurring within heterozygous insertions do not stimulate interhomologue recombination. ..
- Graham V, Khudyakov J, Ellis P, Pevny L. SOX2 functions to maintain neural progenitor identity. Neuron. 2003;39:749-65 pubmed..Taken together, these data indicate that SOXB1 signaling is both necessary and sufficient to maintain panneural properties of neural progenitor cells. ..
- Yancey Wrona J, Matson S. Bound Lac repressor protein differentially inhibits the unwinding reactions catalyzed by DNA helicases. Nucleic Acids Res. 1992;20:6713-21 pubmed..In addition, we have shown that Rep protein displaces the bound repressor protein during the course of an unwinding reaction. ..
- Stansel R, Subramanian D, Griffith J. p53 binds telomeric single strand overhangs and t-loop junctions in vitro. J Biol Chem. 2002;277:11625-8 pubmed..These results suggest that p53 has an active role in telomere maintenance and structure through association with the t-loop junction. ..
- Meier B, Clejan I, Liu Y, Lowden M, Gartner A, Hodgkin J, et al. trt-1 is the Caenorhabditis elegans catalytic subunit of telomerase. PLoS Genet. 2006;2:e18 pubmed..elegans. These findings illustrate effects of telomere dysfunction in C. elegans mutants lacking the catalytic subunit of telomerase, trt-1. ..
- Zhang L, Pagano J. IRF-7, a new interferon regulatory factor associated with Epstein-Barr virus latency. Mol Cell Biol. 1997;17:5748-57 pubmed..The identification of a functional viral ISRE and association of IRF-7 with type III latency may be relevant to the mechanism of regulation of Qp. ..
- Kang T, Sancar A. Circadian regulation of DNA excision repair: implications for chrono-chemotherapy. Cell Cycle. 2009;8:1665-7 pubmed..Here we review the significance of the connection linking the circadian clock with nucleotide excision repair and discuss potential implications for chemotherapy. ..
- Dietrich R, Richberg M, Schmidt R, Dean C, Dangl J. A novel zinc finger protein is encoded by the Arabidopsis LSD1 gene and functions as a negative regulator of plant cell death. Cell. 1997;88:685-94 pubmed..We propose that LSD1 regulates transcription, via either repression of a prodeath pathway or activation of an antideath pathway, in response to signals emanating from cells undergoing pathogen-induced hypersensitive cell death. ..
- Adamson A, Kenney S. Epstein-barr virus immediate-early protein BZLF1 is SUMO-1 modified and disrupts promyelocytic leukemia bodies. J Virol. 2001;75:2388-99 pubmed..These results suggest that disruption of PML bodies is important for efficient lytic replication of EBV. Furthermore, Z may potentially alter the function of a variety of cellular proteins by inhibiting SUMO-1 modification...
- Beach D, Bloom K. ASH1 mRNA localization in three acts. Mol Biol Cell. 2001;12:2567-77 pubmed
- Chastain P, Bowers J, Lee D, Bell S, Griffith J. Mapping subunit location on the Saccharomyces cerevisiae origin recognition complex free and bound to DNA using a novel nanoscale biopointer. J Biol Chem. 2004;279:36354-62 pubmed..This versatile, new approach to mapping protein structure has potential for many applications. ..
- Wu M, Tian Q, Reed J. Arabidopsis microRNA167 controls patterns of ARF6 and ARF8 expression, and regulates both female and male reproduction. Development. 2006;133:4211-8 pubmed..The essential patterning function of miR167 contrasts with cases from animals in which miRNAs reinforce or maintain transcriptionally established gene expression patterns. ..
- Unsal Kaçmaz K, Makhov A, Griffith J, Sancar A. Preferential binding of ATR protein to UV-damaged DNA. Proc Natl Acad Sci U S A. 2002;99:6673-8 pubmed..In addition, damaged DNA stimulates the kinase activity of ATR to a significantly higher level than undamaged DNA. Our data suggest that ATR may function as an initial sensor in the DNA damage checkpoint response. ..
- Cheshire J, Baldwin A. Synergistic activation of NF-kappaB by tumor necrosis factor alpha and gamma interferon via enhanced I kappaB alpha degradation and de novo I kappaBbeta degradation. Mol Cell Biol. 1997;17:6746-54 pubmed
- Ellis P, Fagan B, Magness S, Hutton S, Taranova O, Hayashi S, et al. SOX2, a persistent marker for multipotential neural stem cells derived from embryonic stem cells, the embryo or the adult. Dev Neurosci. 2004;26:148-65 pubmed..These studies demonstrate that SOX2 may meet the requirements of a universal neural stem cell marker and provides a means to identify cells which fulfill the basic criteria of a stem cell: self-renewal and multipotent differentiation. ..
- Wang H, Yang Y, Schofield M, Du C, Fridman Y, Lee S, et al. DNA bending and unbending by MutS govern mismatch recognition and specificity. Proc Natl Acad Sci U S A. 2003;100:14822-7 pubmed..Our results provide a structural explanation for the long-standing question of how MutS achieves mismatch repair specificity. ..
- Oğrünç M, Sancar A. Identification and characterization of human MUS81-MMS4 structure-specific endonuclease. J Biol Chem. 2003;278:21715-20 pubmed..Using immunoaffinity purification we show that hMUS81 or hMMS4 alone have no detectable nuclease activity, but that the hMUS81.hMMS4 complex is a structure-specific nuclease that is capable of resolving fork structures. ..
- Wagner E, Ospina J, Hu Y, Dundr M, Matera A, Marzluff W. Conserved zinc fingers mediate multiple functions of ZFP100, a U7snRNP associated protein. RNA. 2006;12:1206-18 pubmed..Comparisons with other mammalian ZFP100 orthologs show that the central Zn fingers sufficient for in vivo activity are most highly conserved, whereas the number and sequence of the Zn fingers in the N- and C-terminal domains vary. ..
- Reardon J, Sancar A. Repair of DNA-polypeptide crosslinks by human excision nuclease. Proc Natl Acad Sci U S A. 2006;103:4056-61 pubmed..Our data suggest that, if coupled with proteolytic degradation of the crosslinked protein, the human excision nuclease may be the major enzyme system for eliminating protein-DNA crosslinks from the genome. ..
- Lockman K, Hinson J, Medlin M, Morris D, Taylor J, Mack C. Sphingosine 1-phosphate stimulates smooth muscle cell differentiation and proliferation by activating separate serum response factor co-factors. J Biol Chem. 2004;279:42422-30 pubmed..Taken together these results demonstrate that S1P activates multiple signaling pathways in SMC and regulates proliferation by ERK-dependent activation of Elk-1 and differentiation by RhoA-dependent activation of MRTF-A. ..
- Thrower D, Bloom K. Dicentric chromosome stretching during anaphase reveals roles of Sir2/Ku in chromatin compaction in budding yeast. Mol Biol Cell. 2001;12:2800-12 pubmed..The association of Sir2 with the affected region suggests a direct role in this process, which may include the formation of heterochromatic DNA. ..
- Liao M, Zhang X, Hill R, Gao J, Qumsiyeh M, Nichols W, et al. No requirement for V(D)J recombination in p53-deficient thymic lymphoma. Mol Cell Biol. 1998;18:3495-501 pubmed
- Wagner E, Marzluff W. ZFP100, a component of the active U7 snRNP limiting for histone pre-mRNA processing, is required for entry into S phase. Mol Cell Biol. 2006;26:6702-12 pubmed
- Tolbert D, Lu X, Yin C, Tantama M, van Dyke T. p19(ARF) is dispensable for oncogenic stress-induced p53-mediated apoptosis and tumor suppression in vivo. Mol Cell Biol. 2002;22:370-7 pubmed..These results underscore the complexity of p53 tumor suppression and further indicate the existence of distinct cell-specific pathways that respond to similar stimuli. ..
- Larocque J, Dougherty D, Hussain S, Sekelsky J. Reducing DNA polymerase alpha in the absence of Drosophila ATR leads to P53-dependent apoptosis and developmental defects. Genetics. 2007;176:1441-51 pubmed..This work demonstrates a critical contribution of the checkpoint function of MEI-41 in responding to endogenous damage. ..
- Aviv D, Rusterucci C, Holt B, Dietrich R, Parker J, Dangl J. Runaway cell death, but not basal disease resistance, in lsd1 is SA- and NIM1/NPR1-dependent. Plant J. 2002;29:381-91 pubmed..Continued escalation of signaling in the absence of LSD1 leads to runaway cell death. We propose that LSD1 is a key negative regulator of this signal potentiation. ..
- Liu J, Furukawa M, Matsumoto T, Xiong Y. NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Mol Cell. 2002;10:1511-8 pubmed..We suggest that by restricting SKP1-CUL1 interaction, CAND1 regulated the assembly of productive SCF ubiquitin ligases, allowing a common CUL1-ROC core to be utilized by a large number of SKP1-F box-substrate subcomplexes. ..
- Reuther G, Lambert Q, Booden M, Wennerberg K, Becknell B, Marcucci G, et al. Leukemia-associated Rho guanine nucleotide exchange factor, a Dbl family protein found mutated in leukemia, causes transformation by activation of RhoA. J Biol Chem. 2001;276:27145-51 pubmed..These data demonstrate that LARG is the first functional Dbl protein mutated in cancer and indicate LARG-mediated activation of RhoA may play a role in the development of human leukemias. ..
- Patel T, Kramer I, Kucera J, Niederkofler V, Jessell T, Arber S, et al. Peripheral NT3 signaling is required for ETS protein expression and central patterning of proprioceptive sensory afferents. Neuron. 2003;38:403-16 pubmed..Finally, addition of NT3 to DRG explant cultures resulted in induction of ER81 protein. Our data indicate that NT3 mediates the formation of proprioceptive afferent-motor neuron connections via regulation of ER81. ..
- Mendonca V, Matson S. Genetic analysis of delta helD and delta uvrD mutations in combination with other genes in the RecF recombination pathway in Escherichia coli: suppression of a ruvB mutation by a uvrD deletion. Genetics. 1995;141:443-52 pubmed..These data are discussed in the context of two "RecF" homologous recombination pathways operating in a recBCsbcB(C) strain background. ..
- Reardon J, Ge H, Gibbs E, Sancar A, Hurwitz J, Pan Z. Isolation and characterization of two human transcription factor IIH (TFIIH)-related complexes: ERCC2/CAK and TFIIH. Proc Natl Acad Sci U S A. 1996;93:6482-7 pubmed..These data suggest that TFIIH* and ERCC2/CAK interact to form the TFIIH holoenzyme capable of efficiently assembling the pol II transcription initiation complex and directly participating in excision repair reactions. ..
- Spychala J, Kitajewski J. Wnt and beta-catenin signaling target the expression of ecto-5'-nucleotidase and increase extracellular adenosine generation. Exp Cell Res. 2004;296:99-108 pubmed..Our study demonstrates a link between the Wnt signaling and the regulation of two enzymes that control the metabolism of adenosine. ..
- Cao R, Zhang Y. SUZ12 is required for both the histone methyltransferase activity and the silencing function of the EED-EZH2 complex. Mol Cell. 2004;15:57-67 pubmed..Thus, our study establishes a critical role of SUZ12 in H3-lysine 27 methylation and Hox gene silencing. ..
- Yeh E, Bloom K. Hitching a ride. EMBO Rep. 2006;7:985-7 pubmed
- Unsal Kaçmaz K, Mullen T, Kaufmann W, Sancar A. Coupling of human circadian and cell cycles by the timeless protein. Mol Cell Biol. 2005;25:3109-16 pubmed
- Grevengoed E, Fox D, Gates J, Peifer M. Balancing different types of actin polymerization at distinct sites: roles for Abelson kinase and Enabled. J Cell Biol. 2003;163:1267-79 pubmed..We also examined other actin regulators. Loss of Abl leads to changes in the localization of the Arp2/3 complex and the formin Diaphanous, and mutations in diaphanous or capping protein beta enhance abl phenotypes. ..
- Donaldson T, Duronio R. Cancer cell biology: Myc wins the competition. Curr Biol. 2004;14:R425-7 pubmed
- Bessho T. Nucleotide excision repair 3' endonuclease XPG stimulates the activity of base excision repairenzyme thymine glycol DNA glycosylase. Nucleic Acids Res. 1999;27:979-83 pubmed..These results provide in vitro evidence for a second function of XPG protein in DNA repair and a mechanistic basis for its stimulatory activity on HsNTH protein. ..