Genomes and Genes
Experts and Doctors on cell line in Chapel Hill, North Carolina, United States
Locale: Chapel Hill, North Carolina, United States
Topic: cell line
Publications323 found, 100 shown here
- Koller B, Smithies O. Inactivating the beta 2-microglobulin locus in mouse embryonic stem cells by homologous recombination. Proc Natl Acad Sci U S A. 1989;86:8932-5 pubmed..Breeding of offspring from such animals will allow investigation of the effects of homozygous loss of beta 2-microglobulin. ..
- Howell B, Hoffman D, Fang G, Murray A, Salmon E. Visualization of Mad2 dynamics at kinetochores, along spindle fibers, and at spindle poles in living cells. J Cell Biol. 2000;150:1233-50 pubmed..Finally, in normal cells, the half-life of Mad2 turnover at poles, 23 s, was similar to kinetochores. Thus, kinetochore-derived sites along spindle fibers and at spindle poles may also catalyze Mad2 inhibitory complex formation. ..
- Shackelford J, Pagano J. Tumor viruses and cell signaling pathways: deubiquitination versus ubiquitination. Mol Cell Biol. 2004;24:5089-93 pubmed
- Nalbant P, Hodgson L, Kraynov V, Toutchkine A, Hahn K. Activation of endogenous Cdc42 visualized in living cells. Science. 2004;305:1615-9 pubmed
- Stalheim L, Ding Y, Gullapalli A, Paing M, Wolfe B, Morris D, et al. Multiple independent functions of arrestins in the regulation of protease-activated receptor-2 signaling and trafficking. Mol Pharmacol. 2005;67:78-87 pubmed..These findings further suggest that the diverse functions of arrestins in regulating PAR2 signaling and trafficking are controlled by multiple independent interactions involving both the intracellular loops and the C-tail. ..
- Wang Y, Xu L, Duan H, Pasek D, Eu J, Meissner G. Knocking down type 2 but not type 1 calsequestrin reduces calcium sequestration and release in C2C12 skeletal muscle myotubes. J Biol Chem. 2006;281:15572-81 pubmed..Taken together, our data suggest that knocking down CSQ2, but not CSQ1, leads to reduced Ca2+ storage and release in C2C12 myotubes. ..
- Higgins M, Loiselle D, Haystead T, Graves L. Human cytidine triphosphate synthetase 1 interacting proteins. Nucleosides Nucleotides Nucleic Acids. 2008;27:850-7 pubmed publisher..Thus, these studies provide novel information on the potential interacting proteins that may regulate CTPS1 function or intracellular localization. ..
- Kang T, Lindsey Boltz L, Reardon J, Sancar A. Circadian control of XPA and excision repair of cisplatin-DNA damage by cryptochrome and HERC2 ubiquitin ligase. Proc Natl Acad Sci U S A. 2010;107:4890-5 pubmed publisher..These findings may be used as a guide for timing of cisplatin chemotherapy. ..
- Ryman K, Klimstra W, Nguyen K, Biron C, Johnston R. Alpha/beta interferon protects adult mice from fatal Sindbis virus infection and is an important determinant of cell and tissue tropism. J Virol. 2000;74:3366-78 pubmed..Ablation of the IFN-alpha/beta system alters the apparent cell and tissue tropism of the virus and renders macrophage-DC-lineage cells permissive to infection. ..
- Hardman R, Afshari C, Barrett J. Involvement of mammalian MLH1 in the apoptotic response to peroxide-induced oxidative stress. Cancer Res. 2001;61:1392-7 pubmed..Together, our data indicate that cells lacking MLH1 may possess a selective growth advantage under oxidatively stressed conditions via the disregulation of apoptosis, possibly involving the mitochondria. ..
- Lambert J, Lambert Q, Reuther G, Malliri A, Siderovski D, Sondek J, et al. Tiam1 mediates Ras activation of Rac by a PI(3)K-independent mechanism. Nat Cell Biol. 2002;4:621-5 pubmed..Furthermore, activated Ras and Tiam1 cooperate to cause synergistic formation of Rac-GTP in a PI(3)K-independent manner. Thus, Tiam1 can function as an effector that directly mediates Ras activation of Rac. ..
- Loomis R, Holmes D, Elms A, Solski P, Der C, Su L. Citron kinase, a RhoA effector, enhances HIV-1 virion production by modulating exocytosis. Traffic. 2006;7:1643-53 pubmed..Finally, citron-K promotes exocytosis of microvesicles or exosomes that co-purify with HIV-1 virions. We conclude that citron-K enhances HIV-1 virion production by stimulating the endosomal compartments and exocytosis. ..
- Lee C, Hickey M, Sanford C, McGuire C, Cowey C, Simon M, et al. VHL Type 2B gene mutation moderates HIF dosage in vitro and in vivo. Oncogene. 2009;28:1694-705 pubmed publisher..Our experiments support a model in which the representative Type 2B R167Q mutant pVhl produces a unique profile of HIF dysregulation, thereby promoting tissue-specific effects on cell growth, development and tumor predisposition. ..
- Furukawa M, Zhang Y, McCarville J, Ohta T, Xiong Y. The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1. Mol Cell Biol. 2000;20:8185-97 pubmed..This pathway may be commonly utilized for the assembly of other cullin ligases. ..
- Curtis K, Yount B, Baric R. Heterologous gene expression from transmissible gastroenteritis virus replicon particles. J Virol. 2002;76:1422-34 pubmed..The development of infectious TGEV replicon particles should assist studies of TGEV replication and assembly as well as facilitate the production of novel swine candidate vaccines. ..
- Baric R, Yount B, Lindesmith L, Harrington P, Greene S, Tseng F, et al. Expression and self-assembly of norwalk virus capsid protein from venezuelan equine encephalitis virus replicons. J Virol. 2002;76:3023-30 pubmed..Mutations located in the N-terminal and P1 domains of the NV capsid protein ablated capsid self-assembly in mammalian cells. ..
- Shannon K, Canman J, Salmon E. Mad2 and BubR1 function in a single checkpoint pathway that responds to a loss of tension. Mol Biol Cell. 2002;13:3706-19 pubmed..We conclude that reduced tension at kinetochores with a full complement of kinetochore microtubules induces a checkpoint dependent metaphase delay associated with elevated amounts of kinetochore 3F3/2, Bub1, and BubR1 labeling. ..
- DeMali K, Barlow C, Burridge K. Recruitment of the Arp2/3 complex to vinculin: coupling membrane protrusion to matrix adhesion. J Cell Biol. 2002;159:881-91 pubmed..The recruitment of the Arp2/3 complex to vinculin may be one mechanism through which actin polymerization and membrane protrusion are coupled to integrin-mediated adhesion. ..
- Mahajan K, Mitchell B. Role of human Pso4 in mammalian DNA repair and association with terminal deoxynucleotidyl transferase. Proc Natl Acad Sci U S A. 2003;100:10746-51 pubmed..Loss of hPso4 expression induced by siRNA results in accumulation of DSBs, apoptosis, and decreased cell survival after DNA damage. We conclude that hPso4 plays a major and previously undefined role in mammalian DNA DSB repair. ..
- Gardner O, Dewar B, Earp H, Samet J, Graves L. Dependence of peroxisome proliferator-activated receptor ligand-induced mitogen-activated protein kinase signaling on epidermal growth factor receptor transactivation. J Biol Chem. 2003;278:46261-9 pubmed..In summary, PPARalpha and gamma ligands activate two distinct signaling cascades in GN4 cells leading to MAPK activation. ..
- Paul D, Harmon A, Winston C, Patel Y. Calpain facilitates GLUT4 vesicle translocation during insulin-stimulated glucose uptake in adipocytes. Biochem J. 2003;376:625-32 pubmed..These studies identify calpain as a novel factor involved in GLUT4 vesicle trafficking and suggest a link between calpain activity and the development of type 2 diabetes. ..
- Kakoki M, Tsai Y, Kim H, Hatada S, Ciavatta D, Takahashi N, et al. Altering the expression in mice of genes by modifying their 3' regions. Dev Cell. 2004;6:597-606 pubmed..In mice having two functionally different native genes with modified 3'UTRs, the desired expression was obtained. ..
- Hu P, Berkowitz P, Madden V, Rubenstein D. Stabilization of plakoglobin and enhanced keratinocyte cell-cell adhesion by intracellular O-glycosylation. J Biol Chem. 2006;281:12786-91 pubmed..These observations suggest that O-glycosylation functions to regulate the post-translational stability of plakoglobin and keratinocyte cell-cell adhesion. ..
- Qian S, McDonough H, Boellmann F, Cyr D, Patterson C. CHIP-mediated stress recovery by sequential ubiquitination of substrates and Hsp70. Nature. 2006;440:551-5 pubmed
- Guo J, Padilla R, Ambrose W, De Kok I, Cooper L. The effect of hydrofluoric acid treatment of TiO2 grit blasted titanium implants on adherent osteoblast gene expression in vitro and in vivo. Biomaterials. 2007;28:5418-25 pubmed..In vivo assessments of implant adherent cell phenotypes provide further insight into the mechanisms affecting alloplast-tissue interactions...
- Koh J, Hogue J, Wang Y, DiSalvo M, Allbritton N, Shi Y, et al. Single-cell functional analysis of parathyroid adenomas reveals distinct classes of calcium sensing behaviour in primary hyperparathyroidism. J Cell Mol Med. 2016;20:351-9 pubmed publisher..Recognition of quantitative differences in calcium sensing could have important implications for the clinical management of PHPT. ..
- Sorscher D, Barrett J, Cordeiro Stone M. Timing of replication of differentially transcribed genes in Syrian hamster embryo fibroblasts. Mol Carcinog. 1993;8:28-33 pubmed..These results suggest that downregulation of transcription is not always accompanied by a concomitant change in time of gene replication from early to late S phase. ..
- MacDonald G, Johnston R. Role of dendritic cell targeting in Venezuelan equine encephalitis virus pathogenesis. J Virol. 2000;74:914-22 pubmed..A second-site suppressor mutation that restores viral spread to lymphoid tissues and partially restore virulence likewise restored the ability of VEE to infect dendritic cells in vivo...
- Herold C, Qi A, Harden T, Nicholas R. Agonist versus antagonist action of ATP at the P2Y4 receptor is determined by the second extracellular loop. J Biol Chem. 2004;279:11456-64 pubmed
- Morrison T, Mauser A, Klingelhutz A, Kenney S. Epstein-Barr virus immediate-early protein BZLF1 inhibits tumor necrosis factor alpha-induced signaling and apoptosis by downregulating tumor necrosis factor receptor 1. J Virol. 2004;78:544-9 pubmed..Thus, EBV has developed a novel mechanism for evading TNF-alpha antiviral effects during lytic reactivation or primary infection. ..
- Fluet M, Whitmore A, Moshkoff D, Fu K, Tang Y, Collier M, et al. Effects of rapid antigen degradation and VEE glycoprotein specificity on immune responses induced by a VEE replicon vaccine. Virology. 2008;370:22-32 pubmed
- Carter R, Fricks I, Barrett M, Burianek L, Zhou Y, Ko H, et al. Quantification of Gi-mediated inhibition of adenylyl cyclase activity reveals that UDP is a potent agonist of the human P2Y14 receptor. Mol Pharmacol. 2009;76:1341-8 pubmed publisher..We conclude that UDP is an important cognate agonist of the human P2Y14 receptor. ..
- Jeffers L, Madden V, Webster Cyriaque J. BK virus has tropism for human salivary gland cells in vitro: implications for transmission. Virology. 2009;394:183-93 pubmed publisher..These data collectively suggest the potential for BKV oral route of transmission and oral pathogenesis. ..
- Qian K, Kuo R, Chen C, Huang L, Morris Natschke S, Lee K. Anti-AIDS agents 81. Design, synthesis, and structure-activity relationship study of betulinic acid and moronic acid derivatives as potent HIV maturation inhibitors. J Med Chem. 2010;53:3133-41 pubmed publisher..0006 microM), which displayed slightly better activity than 2 as a HIV-1 maturation inhibitor. ..
- Bentz G, Liu R, Hahn A, Shackelford J, Pagano J. Epstein-Barr virus BRLF1 inhibits transcription of IRF3 and IRF7 and suppresses induction of interferon-beta. Virology. 2010;402:121-8 pubmed publisher..These data suggest a new role for BRLF1 with the ability to evade host innate immune responses...
- Wu W, Doreswamy V, Diaz Sanchez D, Samet J, Kesic M, Dailey L, et al. GSTM1 modulation of IL-8 expression in human bronchial epithelial cells exposed to ozone. Free Radic Biol Med. 2011;51:522-9 pubmed publisher..Taken together, GSTM1 deficiency enhances ozone-induced IL-8 production, which is mediated by generated ROS and subsequent NF-ÎºB activation in human bronchial epithelial cells. ..
- Wang J, Williams J, Davis B, Jacobson K, Doerschuk C, Ting J, et al. Monocytic microparticles activate endothelial cells in an IL-1?-dependent manner. Blood. 2011;118:2366-74 pubmed publisher..Therefore, monocytic MPs contain IL-1? and may amplify inflammation by enhancing the activation of the endothelium. ..
- West J, Wicks M, Gregory S, Chugh P, Jacobs S, Zhang Z, et al. An important role for mitochondrial antiviral signaling protein in the Kaposi's sarcoma-associated herpesvirus life cycle. J Virol. 2014;88:5778-87 pubmed publisher..We show that RIG-I and its adaptor protein, MAVS, can sense KSHV infection and that these proteins can suppress KSHV replication following primary infection and/or viral reactivation. ..
- Sakai K, Busby W, Clarke J, Clemmons D. Tissue transglutaminase facilitates the polymerization of insulin-like growth factor-binding protein-1 (IGFBP-1) and leads to loss of IGFBP-1's ability to inhibit insulin-like growth factor-I-stimulated protein synthesis. J Biol Chem. 2001;276:8740-5 pubmed..Polymerization is an important post-translational modification of IGFBP-1 that regulates cellular responses to IGF-I. ..
- Ling Y, Maile L, Lieskovska J, Badley Clarke J, Clemmons D. Role of SHPS-1 in the regulation of insulin-like growth factor I-stimulated Shc and mitogen-activated protein kinase activation in vascular smooth muscle cells. Mol Biol Cell. 2005;16:3353-64 pubmed..We conclude that SHPS-1 functions as an anchor protein that recruits both Shc and SHP-2 and that their recruitment is necessary for IGF-I-dependent Shc phosphorylation, which is required for an optimal mitogenic response in SMCs. ..
- Michaylira C, Simmons J, Ramocki N, Scull B, McNaughton K, Fuller C, et al. Suppressor of cytokine signaling-2 limits intestinal growth and enterotrophic actions of IGF-I in vivo. Am J Physiol Gastrointest Liver Physiol. 2006;291:G472-81 pubmed..We conclude that SOCS2 normally limits basal and IGF-I- and EGF-induced intestinal growth in vivo and has novel inhibitory effects on the IGF-IR tyrosine kinase pathway in intestinal epithelial cells. ..
- Barnes A, Lilley B, Pan Y, Plummer L, Powell A, Raines A, et al. LKB1 and SAD kinases define a pathway required for the polarization of cortical neurons. Cell. 2007;129:549-63 pubmed..Thus, we provide evidence in vivo and in vitro for a multikinase pathway that links extracellular signals to the intracellular machinery required for axon specification. ..
- Kaku M, Mochida Y, Atsawasuwan P, Parisuthiman D, Yamauchi M. Post-translational modifications of collagen upon BMP-induced osteoblast differentiation. Biochem Biophys Res Commun. 2007;359:463-8 pubmed..The results indicate that LHs and LOX/LOXLs are differentially responsive to BMP-induced osteoblast differentiation that may eventually lead to the specific collagen cross-linking pattern seen in bone. ..
- White L, Wang J, Davis N, Johnston R. Role of alpha/beta interferon in Venezuelan equine encephalitis virus pathogenesis: effect of an attenuating mutation in the 5' untranslated region. J Virol. 2001;75:3706-18 pubmed
- Lee D, Pearsall R, Das S, Dey S, Godfrey V, Threadgill D. Epiregulin is not essential for development of intestinal tumors but is required for protection from intestinal damage. Mol Cell Biol. 2004;24:8907-16 pubmed..However, Ereg null mice are highly susceptible to cancer-predisposing intestinal damage caused by oral administration of dextran sulfate sodium. ..
- Yan W, Chen W, Huang L. Mechanism of adjuvant activity of cationic liposome: phosphorylation of a MAP kinase, ERK and induction of chemokines. Mol Immunol. 2007;44:3672-81 pubmed..Our data elucidated one important mechanism of adjuvant activity of cationic liposome and could facilitate rational design of synthetic lipid based adjuvants. ..
- Lee J, Sancar A. Regulation of apoptosis by the circadian clock through NF-kappaB signaling. Proc Natl Acad Sci U S A. 2011;108:12036-41 pubmed publisher..These findings provide a mechanistic foundation for the delayed onset of tumorigenesis in clock-disrupted p53 mutant mice and suggest unique therapeutic strategies for treating cancers associated with p53 mutation. ..
- Fotheringham J, Coalson N, Raab Traub N. Epstein-Barr virus latent membrane protein-2A induces ITAM/Syk- and Akt-dependent epithelial migration through ?v-integrin membrane translocation. J Virol. 2012;86:10308-20 pubmed publisher..The specific effects of LMP2A on an integrin with a diverse repertoire of ligand specificities could promote migration of different cell types and be initiated by multiple chemoattractants. ..
- Joseph D, Adamson M, Kozak C. Genetic mapping of the gene for androgen-binding protein/sex hormone-binding globulin to mouse chromosome 11. Cytogenet Cell Genet. 1991;56:122-4 pubmed..The recent finding that ABP-SHBG is found throughout the rat brain raises the possibility that one of these mutations may be due to a defect in Shbg. ..
- Batra R, Olsen J, Hoganson D, Caterson B, Boucher R. Retroviral gene transfer is inhibited by chondroitin sulfate proteoglycans/glycosaminoglycans in malignant pleural effusions. J Biol Chem. 1997;272:11736-43 pubmed..These results suggest that drainage of the malignant pleural effusion, and perhaps enzymatic pretreatment of the pleural cavity, will be necessary for efficient retroviral vector mediated gene delivery to pleural metastases. ..
- Kim Y, Reed W, Wu W, Bromberg P, Graves L, Samet J. Zn2+-induced IL-8 expression involves AP-1, JNK, and ERK activities in human airway epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2006;290:L1028-35 pubmed..These results suggested that Zn(2+)-induced inhibition of phosphatase activity is an initiating event in MAPK and AP-1 activation that leads to enhanced IL-8 expression by human airway epithelial cells. ..
- Jensen N, Rodriguiz R, Caron M, Wetsel W, Rothman R, Roth B. N-desalkylquetiapine, a potent norepinephrine reuptake inhibitor and partial 5-HT1A agonist, as a putative mediator of quetiapine's antidepressant activity. Neuropsychopharmacology. 2008;33:2303-12 pubmed..Possible contributions of this metabolite to the side effects of quetiapine are discussed. ..
- Nagaoka H, Mochida Y, Atsawasuwan P, Kaku M, Kondoh T, Yamauchi M. 1,25(OH)2D3 regulates collagen quality in an osteoblastic cell culture system. Biochem Biophys Res Commun. 2008;377:674-678 pubmed publisher..These results demonstrate that 1,25(OH)(2)D(3) directly regulates collagen cross-linking in this culture system likely by upregulating gene expression of specific LH and LOX enzymes. ..
- Yan F, Bikbulatov R, Mocanu V, Dicheva N, Parker C, Wetsel W, et al. Structure-based design, synthesis, and biochemical and pharmacological characterization of novel salvinorin A analogues as active state probes of the kappa-opioid receptor. Biochemistry. 2009;48:6898-908 pubmed publisher..These studies provide direct evidence of the presence of a free cysteine in the agonist-bound state of the KOR and provide novel insights into the mechanism by which salvinorin A binds to and activates the KOR. ..
- Jacobs D, Weigert R, Grode K, Donaldson J, Cheney R. Myosin Vc is a molecular motor that functions in secretory granule trafficking. Mol Biol Cell. 2009;20:4471-88 pubmed publisher..These results provide the first live-cell imaging of Myo5c and indicate that Myo5c functions in secretory granule trafficking. ..
- Short D, Trotter K, Reczek D, Kreda S, Bretscher A, Boucher R, et al. An apical PDZ protein anchors the cystic fibrosis transmembrane conductance regulator to the cytoskeleton. J Biol Chem. 1998;273:19797-801 pubmed
- Laakko T, Juliano R. Adhesion regulation of stromal cell-derived factor-1 activation of ERK in lymphocytes by phosphatases. J Biol Chem. 2003;278:31621-8 pubmed..Therefore, these data suggest that adhesion influences SDF-1 activation of ERK by regulating the activity of ERK phosphatases. This identifies a novel locus of adhesion regulation of the ERK cascade. ..
- Griffin C, Trejo J, Magnuson T. Genetic evidence for a mammalian retromer complex containing sorting nexins 1 and 2. Proc Natl Acad Sci U S A. 2005;102:15173-7 pubmed..Moreover, we find that mammalian retromer complexes containing SNX1 and SNX2 have an essential role in embryonic development that is independent of cation-independent mannose 6-phosphate receptor trafficking. ..
- Medlin M, Staus D, Dubash A, Taylor J, Mack C. Sphingosine 1-phosphate receptor 2 signals through leukemia-associated RhoGEF (LARG), to promote smooth muscle cell differentiation. Arterioscler Thromb Vasc Biol. 2010;30:1779-86 pubmed publisher..These data indicate that S1PR2-dependent activation of RhoA in SMC is mediated by LARG and that this signaling mechanism promotes the differentiated SMC phenotype. ..
- Ito S, Shen L, Dai Q, Wu S, Collins L, Swenberg J, et al. Tet proteins can convert 5-methylcytosine to 5-formylcytosine and 5-carboxylcytosine. Science. 2011;333:1300-3 pubmed publisher..Thus, we identify two previously unknown cytosine derivatives in genomic DNA as the products of Tet proteins. Our study raises the possibility that DNA demethylation may occur through Tet-catalyzed oxidation followed by decarboxylation. ..
- Cordeiro Stone M, McNulty J, Sproul C, Chastain P, Gibbs Flournoy E, Zhou Y, et al. Effective intra-S checkpoint responses to UVC in primary human melanocytes and melanoma cell lines. Pigment Cell Melanoma Res. 2016;29:68-80 pubmed publisher..The results indicate that bypass of oncogene-induced senescence during melanoma development was not associated with inactivation of the intra-S checkpoint response to UVC-induced DNA replication stress. ..
- Buhusi M, Midkiff B, Gates A, Richter M, Schachner M, Maness P. Close homolog of L1 is an enhancer of integrin-mediated cell migration. J Biol Chem. 2003;278:25024-31 pubmed..These results support a role for CHL1 in integrin-dependent cell migration that may be physiologically important in regulating cell migration in nerve regeneration and cortical development. ..
- Qian L, Xu Z, Zhang W, Wilson B, Hong J, Flood P. Sinomenine, a natural dextrorotatory morphinan analog, is anti-inflammatory and neuroprotective through inhibition of microglial NADPH oxidase. J Neuroinflammation. 2007;4:23 pubmed..These findings suggest a novel therapy to treat inflammation-mediated neurodegenerative diseases. ..
- Nishimoto J, Nanba E, Inui K, Okada S, Suzuki K. GM1-gangliosidosis (genetic beta-galactosidase deficiency): identification of four mutations in different clinical phenotypes among Japanese patients. Am J Hum Genet. 1991;49:566-74 pubmed..The prediction that these mutations are responsible for the clinical disease was confirmed by no expression of the catalytic activity of the mutant proteins in the COS-I cell expression system.(ABSTRACT TRUNCATED AT 250 WORDS)..
- Satoh M, Reeves W. Induction of lupus-associated autoantibodies in BALB/c mice by intraperitoneal injection of pristane. J Exp Med. 1994;180:2341-6 pubmed
- Partch C, Shields K, Thompson C, Selby C, Sancar A. Posttranslational regulation of the mammalian circadian clock by cryptochrome and protein phosphatase 5. Proc Natl Acad Sci U S A. 2006;103:10467-10472 pubmed publisher..Collectively, these findings indicate that PP5, CKIepsilon, and cryptochrome dynamically regulate the mammalian circadian clock. ..
- Rigby R, Simmons J, Greenhalgh C, Alexander W, Lund P. Suppressor of cytokine signaling 3 (SOCS3) limits damage-induced crypt hyper-proliferation and inflammation-associated tumorigenesis in the colon. Oncogene. 2007;26:4833-41 pubmed..We conclude that cytokine induction of SOCS3 normally provides an intrinsic mechanism to limit injury-induced crypt hyperproliferation and inflammation-associated colon cancer by regulating both STAT3 and NF-kappaB pathways. ..
- Check J, Byrd C, Menio J, Rippe R, Hines I, Wheeler M. Src kinase participates in LPS-induced activation of NADPH oxidase. Mol Immunol. 2010;47:756-62 pubmed publisher..These signaling events fill critical gaps in our understanding of LPS-induced free radical production as well as may potentially responsible for the mechanism of innate immune tolerance or desensitization caused by steroids or ethanol. ..
- Benton M, Rager J, Smeester L, Fry R. Comparative genomic analyses identify common molecular pathways modulated upon exposure to low doses of arsenic and cadmium. BMC Genomics. 2011;12:173 pubmed publisher..These results highlight that even at low levels of exposure both metals can dramatically influence the expression of important cellular pathways. ..
- Bentz G, Whitehurst C, Pagano J. Epstein-Barr virus latent membrane protein 1 (LMP1) C-terminal-activating region 3 contributes to LMP1-mediated cellular migration via its interaction with Ubc9. J Virol. 2011;85:10144-53 pubmed publisher..We propose that LMP1, by interaction with Ubc9, modulates sumoylation processes, which regulate signal transduction pathways that affect phenotypic changes associated with oncogenesis. ..
- Endo K, Shackelford J, Aga M, Yoshizaki T, Pagano J. Upregulation of special AT-rich-binding protein 1 by Epstein-Barr virus latent membrane protein 1 in human nasopharyngeal cells and nasopharyngeal cancer. J Gen Virol. 2013;94:507-13 pubmed publisher..Taken together, our results suggest that SATB1 functions as a pro-metastatic effector of LMP1 signalling in EBV-positive NPC. ..
- Ashburner B, Westerheide S, Baldwin A. The p65 (RelA) subunit of NF-kappaB interacts with the histone deacetylase (HDAC) corepressors HDAC1 and HDAC2 to negatively regulate gene expression. Mol Cell Biol. 2001;21:7065-77 pubmed..Moreover, it suggests that the association of NF-kappaB with the HDAC1 and HDAC2 corepressor proteins functions to repress expression of NF-kappaB-regulated genes as well as to control the induced level of expression of these genes. ..
- Mochida Y, Parisuthiman D, Kaku M, Hanai J, Sukhatme V, Yamauchi M. Nephrocan, a novel member of the small leucine-rich repeat protein family, is an inhibitor of transforming growth factor-beta signaling. J Biol Chem. 2006;281:36044-51 pubmed..Taken together, NPN is a novel member of the SLRP family that may play important roles in kidney development and pathophysiology by functioning as an endogenous inhibitor of TGF-beta signaling. ..
- Dan H, Adli M, Baldwin A. Regulation of mammalian target of rapamycin activity in PTEN-inactive prostate cancer cells by I kappa B kinase alpha. Cancer Res. 2007;67:6263-9 pubmed..The results indicate a novel role for IKK alpha in controlling mTOR function in cancer cells with constitutive Akt activity. ..
- Lu D, Garcia Contreras L, Xu D, Kurtz S, Liu J, Braunstein M, et al. Poly (lactide-co-glycolide) microspheres in respirable sizes enhance an in vitro T cell response to recombinant Mycobacterium tuberculosis antigen 85B. Pharm Res. 2007;24:1834-43 pubmed..These results are a foundation for further investigation into the potential use of PLGA particles for delivery of vaccines to prevent M. tuberculosis infection. ..
- Wu C, Asokan S, Berginski M, Haynes E, Sharpless N, Griffith J, et al. Arp2/3 is critical for lamellipodia and response to extracellular matrix cues but is dispensable for chemotaxis. Cell. 2012;148:973-87 pubmed publisher..Consistent with this finding, cells depleted of Arp2/3 fail to globally align focal adhesions, suggesting that one principle function of lamellipodia is to organize cell-matrix adhesions in a spatially coherent manner...
- Mendonça D, Mendonça G, Cooper L. Mammalian two-hybrid assays for studies of interaction of p300 with transcription factors. Methods Mol Biol. 2013;977:323-38 pubmed publisher..Here we describe the methods to study the interaction of p300 with other transcription factors, specifically between p300 and two transcription factors related to hypoxia and inflammation, HIF-1? and NF-?B-p65, respectively. ..
- Li G, Harton J, Zhu X, Ting J. Downregulation of CIITA function by protein kinase a (PKA)-mediated phosphorylation: mechanism of prostaglandin E, cyclic AMP, and PKA inhibition of class II major histocompatibility complex expression in monocytic lines. Mol Cell Biol. 2001;21:4626-35 pubmed..This is the first demonstration that the posttranslational modification of CIITA mediates inhibition of class II MHC transcription. ..
- Casper K, Jones K, McCarthy K. Characterization of astrocyte-specific conditional knockouts. Genesis. 2007;45:292-9 pubmed..Additionally, our system is shown to successfully recombine a floxed Connexin43 locus, although recombination is not as extensive as seen in crosses with reporter lines. ..
- Frieman M, Ratia K, Johnston R, Mesecar A, Baric R. Severe acute respiratory syndrome coronavirus papain-like protease ubiquitin-like domain and catalytic domain regulate antagonism of IRF3 and NF-kappaB signaling. J Virol. 2009;83:6689-705 pubmed publisher..The potential mechanism of PLP antagonism and its role in pathogenesis are discussed. ..
- Gherghe C, Leonard C, Gorelick R, Weeks K. Secondary structure of the mature ex virio Moloney murine leukemia virus genomic RNA dimerization domain. J Virol. 2010;84:898-906 pubmed publisher..These experiments support well-defined structural models for an authentic dimerization domain and also emphasize that many features of mature genomic RNA dimers can be reproduced in vitro using properly designed, simplified RNAs...
- Currie J, Stewman S, Schimizzi G, Slep K, Ma A, Rogers S. The microtubule lattice and plus-end association of Drosophila Mini spindles is spatially regulated to fine-tune microtubule dynamics. Mol Biol Cell. 2011;22:4343-61 pubmed publisher..These novel microtubule contact sites are necessary for the interplay between the conserved TOG domains and inter-TOG MT binding that underlies the ability of Msps to promote MT dynamic instability. ..
- Martin T, Mitin N, Cox A, Yeh J, Der C. Phosphorylation by protein kinase C? regulates RalB small GTPase protein activation, subcellular localization, and effector utilization. J Biol Chem. 2012;287:14827-36 pubmed publisher..In summary, our data suggest that phosphorylation by PKC? is critical for RalB-mediated vesicle trafficking and exocytosis. ..